Radionuclides and heavy metal contamination in edible fish and shellfish from two Bay of Bengal estuaries: source apportionment and health risk assessment.

Integrated oxidative, mitochondrial, and apoptotic responses to ammonia-hypoxia co-toxicity in Macrobrachium rosenbergii muscle.

Effects of dietary microalgae supplementation at different levels on Macrobrachium rosenbergii insect meal-based diets: Antioxidant capacity, hepatopancreatic morphology, and carotenoid retention

Identification and full genome characterization of a novel CRESS DNA virus associated with giant freshwater prawn, Macrobrachium Rosenbergii.

Searching publicly archived sequence data for emerging aquatic animal pathogens is a powerful but challenging approach for increasing our understanding of newly identified or poorly characterized organisms. However, searching for target sequences within the sequence read archive (SRA) database requires significant time, data storage, and computing power, limiting its accessibility. Utilizing a new database, Logan, we undertook a meta-analysis of SRA data sets to investigate the presence of an emerging virus, Macrobrachium rosenbergii golda virus (MrGV). MrGV was first characterized in M. rosenbergii larvae in 2020, associated with repeated mass mortalities in Bangladesh hatcheries. MrGV has since been detected in two separate reports from the Jiangsu Province of central, coastal China, and during a larval mortality event in India. Here, we discovered that MrGV is present in two additional provinces in southern China, Thailand, and India. We also found molecular evidence to confirm, as previously suspected, the circulation of the virus within Southern Asian populations of M. rosenbergii as far back as 2011, and that, based on relative abundance, MrGV is mostly associated with larvae. Overall, the identification of MrGV sequences in data sets that are largely unpublished within the scientific literature has provided novel insights into the pathogen's biology, including the prevalence of MrGV globally and the life stages of prawns that should be screened to prevent the spread of the virus. This work illustrates how mining public sequencing data, supported by databases like Logan and standardized metadata submissions, can support cost-effective epidemiological studies of pathogens and strengthen One Health approaches to global disease monitoring.IMPORTANCESearching for target sequences within the sequence read archive (SRA) database requires significant time, data storage, and computing power, limiting its accessibility. This study demonstrates how the Logan database, constructed from an SRA-wide genome assembly, can be utilized to rapidly and efficiently find target sequences within the SRA database, expanding the use of these publicly available data sets outside of their original intended purposes. Here, we searched for an emerging virus, Macrobrachium rosenbergii golda virus, in prawns to reveal insights into its geographic distribution, host range, and relative abundance, without the need for additional sampling. We demonstrate how, with careful application of this approach, alongside improvements in metadata quality and accessibility, sequencing data sets can be used to uncover critical insights into pathogen biology. This type of data mining could add otherwise unknown data to epidemiological studies of emerging, re-emerging, and rare pathogens globally, allowing the determination of the spread of agents within and between populations.

Corrigendum to “Impact of black soldier fly (Hermetia illucens) larvae meal on giant freshwater prawn (Macrobrachium rosenbergii): Antioxidant capacity, flesh quality, and myofiber development” [Aquaculture, Volume 612, Part 1, 15 January 2026, 743110

Effects of the fishmeal freshness on growth performance, immune response, hepatopancreatic health and anti-stress capacity of giant freshwater prawn (Macrobrachium rosenbergii)

As integral components of aquatic food webs, crustaceans serve as pivotal conduits for energy transfer and sensitive bioindicators of environmental quality. This study characterizes the toxicological consequences of settleable atmospheric particulate matter (SePM)-a byproduct of industrial iron-ore processing-utilizing the freshwater shrimp Macrobrachium rosenbergii as a model organism. Experimental exposure demonstrated that SePM significantly enhances the aqueous solubility of both conventional (Fe, Al, Zn, Cu, Mn) and emergent (Cr, Ni, Sr, Sn, La, Ce) metals, establishing it as a potent vector for multi-elemental contamination. Exposed individuals bioaccumulated 24 metals, with predominant enrichment of Fe, Ba, Sr, Cu, and Zn; these accumulation profiles were modulated by both external concentration and element-specific uptake kinetics. Notably, ultrastructural characterization revealed significant metal sequestration within the exoskeleton, identifying an incidental pathway for contaminant cycling through discarded carapaces (ecdysis). Biochemical assessments indicated impairment of redox homeostasis, characterized by the exhaustion of enzymatic (CAT, SOD, GPx, GST) and non-enzymatic (GSH) defenses, alongside elevated lipid peroxidation and DNA strand breaks. While bioaccumulation had a dose-dependent progression, oxidative distress was manifest even at the lowest exposure levels, underscoring that the toxicity of SePM as a complex mixture cannot be accurately predicted by the additive impacts of its individual constituents. These findings highlight SePM as a critical, yet largely overlooked, driver of air-to-water cross-contamination. Our results underscore the urgent need to incorporate such atmospheric deposition into environmental monitoring frameworks and to expand regulatory criteria to address emergent metals and potential synergistic effects of metallic mixtures.

Immunometabolic regulation in Macrobrachium rosenbergii during Aeromonas caviae infection

Examination of the role of neuropeptide F in promoting food intake, digestive functioning, growth performance, and expression of immune-related genes in juvenile giant freshwater prawn, Macrobrachium rosenbergii

Comprehensive tissue-specific responses to chronic hypoxia in Macrobrachium rosenbergii: A multi-omics perspective

Integrated multi-omics and biochemical insights into ammonia-induced oxidative stress, metabolic disruption, and apoptosis in Macrobrachium rosenbergii gills.

ABSTRACT Bangladesh’s climate is favourable for giant freshwater prawn (Macrobrachium rosenbergii) farming, but conventional practices contribute to high greenhouse gas (GHG) emissions, threatening aquaculture sustainability. Integrated multi-trophic aquaculture (IMTA) offers a promising approach to mitigate GHG by incorporating species that extract excess organic matter from farms. Molluscs, particularly snails, have been suggested as potential extractive species. This study aimed to identify suitable local snail species for IMTA in the southwestern Khulna district across three salinity zones (low, medium, moderately high) during the monsoon (July–October). Sampling covered three waterbody types: aquaculture, non-aquaculture and open waterbodies. Year-round perceptions-based data were obtained through focus group discussions (FGDs), while direct quadrat sampling provided monsoon-season field observations data, identifying nine and seven mollusc species, respectively. Bellamya bengalensis was most abundant in late monsoon within non-aquaculture waterbodies (39.56 ± 12.74 individuals/m²), whereas Pila globosa peaked in mid-monsoon (10.22 ± 3.41 individuals/m²). Snail abundance was highest in undisturbed, vegetated non-aquaculture waterbodies. Distribution patterns were influenced by salinity and water quality parameters. Both B. bengalensis and P. globosa show potential for IMTA for their availability and potential use in feed, with B. bengalensis particularly suited because of its filter-feeding behaviour, adaptability and rapid reproduction.

In recent years, there has been an increasing demand for high-quality dried aquatic products that possess similar organoleptic and nutritional qualities to their fresh counterparts. The present study investigated the effects of hot air drying (HAD), heat pump drying (HPD), vacuum freeze drying (VFD) and microwave vacuum drying (MVD) on drying efficiency, physicochemical and microstructural properties, and volatile compounds of Macrobrachium rosenbergii meat. MVD achieved the highest drying efficiency, whereas VFD resulted in the highest levels of crude protein and astaxanthin contents. VFD also produced higher lightness but lower hardness compared to the other methods. Low-field nuclear magnetic resonance analysis revealed a leftward shift in the transverse relaxation time values of both free and immobilized water as the drying process continued. Scanning electron microscope analysis revealed dense structures with noticeable muscle fiber contractions for HAD and HPD samples, whereas numerous small voids generated for the VFD sample, but larger pores formed for the MVD sample. Fourier transform infrared spectroscopy indicated that VFD better maintained protein structural intergiry. Gas chromatography-mass spectrometry analysis revealed significant differences in volatile compounds, with the key flavors such as 1-octen-3-ol, decyl aldehyde and 1-nonanal present in all samples. Pyrazine compounds contributed roasted and nutty aromas to the HAD, HPD and MVD samples. Overall, MVD is a promising method for dehydration of M. rosenbergii meat. © 2026 Society of Chemical Industry.

The occurrence of the non-native species Macrobrachium rosenbergii has been recorded in multiple drainage basins and estuarine systems throughout Brazil, including 11 coastal Protected Areas. In the Cananéia estuarine–lagoon complex, participatory monitoring conducted between 2015 and 2025 revealed persistent populations and the presence of ovigerous females, indicating local establishment and reproduction. The species exhibits broad tolerance to salinity gradients and marked trophic plasticity, allowing its integration into native ecological networks. Records are concentrated in the Southeast and Northeast regions and are closely associated with aquaculture areas. The presence of this invasive crustacean in sensitive estuarine ecosystems, such as the Atlantic Forest Biosphere Reserve, represents a serious threat to native biodiversity by intensifying competitive interactions, altering trophic dynamics, and increasing sanitary risks, thereby requiring urgent monitoring and management actions.

The family Flaviviridae has been expanded to include the highly divergent flavi-like viruses into three new families, Flaviviridae, Pestiviridae, and Hepaciviridae, in the order Amarillovirales. Classical flavivirids are small, enveloped viruses with positive-sense ssRNA genomes lacking a 3' poly(A) tail and ~9.0-13.0 kb in length, with a single open reading frame (ORF) encoding structural proteins at the N-terminus and nonstructural proteins at the C-terminus. Members infect a wide range of mammals, birds, and insects, and many are host-specific and pathogenic. Although the RNA-directed RNA polymerase (RdRP) gene sequences of the flavi-like viruses group phylogenetically with those of classical flavivirids, flavi-like viruses often encode larger polyproteins and possess substantially longer genomes of up to ~40 kb, and some have a 3' poly(A) tail. Their host range extends across the whole animal kingdom and angiosperm plants. This review describes the reported flavi-like viruses of aquatic animals, providing a meaningful update on all three new families in Amarillovirales that have been discovered using metagenomics in fish, crustaceans, mollusks, and echinoderms. These amarilloviruses include pathogenic viruses of aquatic animals, such as Cyclopterus lumpus virus (CLuV) detected in moribund lumpfish, and infectious precocity virus (IPV) found in iron prawn syndrome (IPS)-affected farmed giant freshwater prawns.

Dietary inclusion of Allium cepa and Allium sativum extract improves growth performance, haematological and immunological indices, body composition and disease resistance of prawn (Macrobrachium rosenbergii) against Vibrio parahaemolyticus infection.

Acute toxicity assessment and real-time metabolic rate responses of early life stage Macrobrachium rosenbergii to ammonia exposures at different salinities.

Design and field validation of thermal anesthetization system for waterless live transport of giant freshwater prawn (Macrobrachium rosenbergii)

Unraveling the critical stage governing quality degradation of Macrobrachium rosenbergii meat during microwave vacuum drying: A multiscale analysis.