Macrobrachium Nipponense Research Articles (Page 1)

This study conducted a bioinformatic analysis of two Hyaluronidase-like isoforms (Mn-HyaL1 and Mn-HyaL2) in Macrobrachium nipponense and investigated their phylogenetic relationships. The open reading frames of Mn-HyaL1 and Mn-HyaL2 were 1101 bp (encoding 366 amino acids) and 1164 bp (encoding 387 amino acids), respectively. Both isoforms exhibited similar conserved domains, with an amino acid sequence similarity of 60.21%. Quantitative PCR analysis revealed that the expression levels of Mn-HyaL1 and Mn-HyaL2 increased during the mid-to-late phase of each developmental stage, were higher during the reproductive season than in the non-reproductive season, and were more abundant in the hepatopancreas than in other tissues. RNA interference experiments targeting both genes simultaneously demonstrated that knockdown of Mn-HyaL2 significantly accelerated ovarian development in M. nipponense, indicating that Mn-HyaL genes function as negative regulators of ovarian maturation. A comparative analysis of multiple genes revealed the following descending order of potency in promoting ovarian development in M. nipponense: Mn-Cholesterol 7-desaturase > Mn-Cathepsin L1. The order of potency in inhibiting ovarian development in M. nipponense, from strongest to weakest, was determined to be Mn-Gonad-inhibiting hormone > Mn-HyaL2.

Effects of stocking density on the oriental river prawn (Macrobrachium nipponense): A comprehensive evaluation of growth, hematological, physio-immunological, and antioxidant responses

China possesses vast saline-alkaline water resources, necessitating their utilization. Macrobrachium nipponense, an economically important freshwater shrimp with notable salinity tolerance, is a candidate for saline aquaculture. This study determined the 96-h LC50 of salinity for the genetically improved "Taihu No. 3" strain juveniles across a gradient (0–30 parts per thousand) and investigated associated stress responses. Morphological, physiological, and molecular responses were analyzed via antioxidant enzyme activity (glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA)) and immune gene expression in hepatopancreas and gills. Results showed the 96-h LC50 was 11.841 ppt, significantly lower than wild populations, suggesting enhanced energy allocation towards growth over osmoregulation in "Taihu No. 3". Acute and chronic stress significantly elevated GPx, GR, CAT, SOD activities and MDA levels (P < 0.05), indicating their critical role in mitigating oxidative damage and maintaining homeostasis. Salinity ≥ 10 ppt induced structural damage, including hepatopancreatic basement membrane disruption and gill alterations (enlarged interlamellar spaces, epithelial swelling). Chronic exposure significantly upregulated immune genes (CAT, Mn-SOD, Cu/Zn-SOD) in both tissues (P < 0.05), demonstrating their involvement in saline acclimation. These findings define measurable salinity tolerance limits and elucidate key immune response mechanisms in "Taihu No. 3", providing a scientific basis for its cultivation in saline-alkaline aquaculture.

Tryptophan, recognized as the third limiting amino acid, plays a crucial physiological function. The optimal tryptophan requirement for oriental river prawn (Macrobrachium nipponense) was assessed, and its impact on agonistic behavior was evaluated in this study. An 8-week feeding trial was implemented with six different tryptophan levels: 0.07%, 0.17%, 0.29%, 0.39%, 0.52%, and 0.64%. The results showed that the highest specific growth rate (SGR) was observed in prawns fed with 0.29% tryptophan. Furthermore, body protein deposition (BPD) and protein efficiency ratio (PER) were significantly elevated in the 0.39% tryptophan group than those in the 0.07% tryptophan group, while the feed conversion rate (FCR) was lowest in the 0.39% tryptophan group. Based on quadratic regression analysis of SGR, BPD, FCR, and PER, the optimal tryptophan requirement was determined to be 0.35%–0.39% of dry matter, 0.90%–1.01% of crude protein. In the 0.52% tryptophan group, crude protein and lipid contents were improved, while ash content was the lowest. The total protein (TP), cholesterol, and urea nitrogen (UN) contents in the hemolymph were observed to be the highest levels in the 0.64% tryptophan group, although there was a reduction in triglyceride (TG) and glucose (GLU) levels. Muscle tor gene expression was the highest at 0.39% tryptophan, with atf4 and atf3 expressions suppressed. Hepatopancreas tor and cpt1 gene mRNA peaked at 0.29% tryptophan, while acc, fas, and atf4 genes were inhibited. Following the feeding trial, prawns fed diets with 0.07%, 0.29%, and 0.64% tryptophan were randomly selected for the detection of agonistic behaviors. Prawns fed 0.29% and 0.64% tryptophan showed less aggression than those fed 0.07%. Serotonin levels were highest in the 0.64% group, followed by 0.29%, and lowest in 0.07%. The 5-ht1b gene expression was significantly increased in the 0.64% tryptophan group compared to the 0.29% and 0.07% tryptophan groups. Both serotonin levels and 5-ht1b expression showed the same significant difference before and after fighting behavior, with increases observed postfight across all treatments. In conclusion, the optimal dietary tryptophan requirement for the oriental river prawn was estimated to be between 0.35% and 0.39% of dry matter (0.90% and 1.01% of crude protein), which enhanced growth performance and effectively reduced agonistic behavior.

In recent years, the number of decapod crustacean species used in aquaculture has continued to increase, and production volumes are growing rapidly, reaching 12,75 million tonnes in 2022. The seven most popular species (Penaeus vannamei, Procambarus clarkii, Eriocheir sinensis, Penaeus monodon, Macrobrachium rosenbergii, Macrobrachium nipponense, Scylla paramamosain) together account for 95% of the world’s total aquaculture production. The specific biology and life cycles of decapod crustacean species determine the possibility of using intensive and extensive farming biotechnologies. At present, thermophilic species have an advantage, for which it is possible to use intensive biotechnologies or a combination of intensive and extensive biotechnologies. The need to use various resources for aquaculture has led to the presence among the most popular species both those for which intensive farming biotechnologies are mainly used, and those for which extensive approaches are used. The possibility of using marine and saltwater reservoirs is an important resource for modern decapod aquaculture. At the same time, freshwater reservoirs and low mineralization reservoirs are widely used, especially in the final stages of cultivation. The most promising species for decapod aquaculture in Russia are those with the highest production rates, which are the most widely used in global aquaculture. At the same time, due to the peculiarities of Russia’s geography and climate, the ability to use a combination of intensive (the use of ultrasound in the early stages of the life cycle) and extensive (the use of ponds for growing to marketable size) approaches to their cultivation is the most imperative condition. In addition, indigenous species of significant commercial importance are an important resource for Russian aquaculture.

BPA disrupts crustacean reproduction: Tissue-specific accumulation, oxidative stress, and gene dysregulation in Macrobrachium nipponense.

Identification of a ficolin involved in ovary reproductive immunity in Macrobrachium nipponense.

Regulation of AMPs expression by six Tolls in stomach during WSSV infection in Macrobrachium nipponense.

Impacts of dietary sodium alginate as a prebiotic on the oriental river prawn (Macrobrachium nipponense): A comprehensive analysis of growth, physiology, immunity, antioxidant, and metabolism

Using vacant saline-alkali land for aquaculture has the advantages of resource utilization and significant economic and ecological benefits, but there is a need to understand the impact of variable alkalinity on aquacultural species. This study investigated the impact of different alkaline stress conditions (10 and 20mmol/L) on transcription and changes in intestinal microbial communities in the oriental river prawn, Macrobrachium nipponense, over a 96-h period. Under low alkalinity conditions, pathways related to carbohydrate metabolism were activated, including glycolysis/gluconeogenesis, mannose metabolism, ascorbate and aldarate metabolism, carbohydrate binding, chitinase activity, and lysosome. Such conditions also led to an increase in the number of beneficial intestinal bacteria, such as Proteobacteria, Firmicutes, Actinobacteriota, and Acidobacteriota. However, high-alkaline conditions inhibited the fibroblast growth factor receptor signaling pathway, store-operated calcium channel activity, and MAPK signaling pathway, and significantly increased the number of pathogenic intestinal bacteria, such as Citrobacter. These results suggest that low alkalinity would promote the growth of M. nipponense by activating the glycolysis pathway and increasing the number of beneficial bacteria. By contrast, high alkalinity would inhibit their immune performance by affecting key signal transduction pathways and increasing harmful bacteria in the intestinal tract. Such insights provide a theoretical basis for the subsequent adaptive aquaculture of M. nipponense in saline-alkali areas.

Identification and ovarian developmental regulation of ribosomal protein S6 kinase in Macrobrachium nipponense.

The growth-stimulatory, immunomodulatory, antioxidative, and metabolic regulation impacts of dietary chitosan supplementation on the oriental river prawn (Macrobrachium nipponense)

Identification of the changes of immune response, morphology, and gene expressions in hepatopancreas of Macrobrachium nipponense under the treatment of alkalinity exposure.

An evaluation of modulatory impacts of dietary zeaxanthin supplementation on Macrobrachium nipponense: Growth, hematological, immunological, and physio-metabolic responses

Macrobrachium nipponense represents a commercial decapod species that predominantly inhabits freshwater ecosystems or environments with low salinity. However, the species exhibits normal survival and reproductive capacity in natural aquatic habitats with salinity levels up to 10 parts per thousand (ppt). The present study aimed to elucidate the molecular mechanisms underlying salinity acclimation in M. nipponense by investigating alterations in oxidative stress, morphological adaptations, and hepatopancreatic gene expression profiles following exposure to a salinity level of 10 ppt. The present study demonstrates that glutathione peroxidase and Na+/K+-ATPase play critical roles in mitigating oxidative stress induced by elevated salinity in M. nipponense. Furthermore, histological analysis revealed distinct pathological alterations in the hepatopancreas of M. nipponense following 7-day salinity exposure, including basement-membrane disruption, luminal expansion, vacuolization, and a marked reduction in storage cells. Transcriptomic profiling of M. nipponense hepatopancreas suggested coordinated activation of both immune (lysosome and protein processing in endoplasmic reticulum pathways) and energy (pyruvate metabolism, glycolysis/gluconeogenesis, and citrate cycle) metabolic processes during salinity acclimation in M. nipponense. Quantitative real-time PCR validation confirmed the reliability of RNA-seq data. This study provides molecular insights into the salinity adaptation mechanisms in M. nipponense, offering potential applications for improving cultivation practices in brackish water environments.

Complementary DNA (cDNA) cloning and expression analysis of selenium glutathione peroxidase (Se-GPx) and glutathione S-transferases (GST) 1 in red claw crayfish (Cherax quadricarinatus).

Buffer zone is an area surrounding a nature reserve that serves to protect the inner ecosystem from external influences. The East Coast Mangrove Forest Reserve is a nature conservation area located in Jambi Province. Both in the waters of Mendahara Ilir and Nipah Panjang, which are focused on preserving mangrove forest ecosystems. The method used is exploratory description and interviews with fishermen. The results obtained show that there are 11 speciesof shrimp found in the East Coast Mangrove Forest Reserve Area, namely: Galah Shrimp (Macrobrachium rosenbergii), Ketak Shrimp (Harpiosquilla raphidea), Hanging Shrimp (Macrobrachium nipponense), Striped Shrimp (Parapenaeopsis sculptilis), Peci Shrimp (Penaeus merguiensis), Lime Shrimp (Metapenaeus dobsoni), Agogo Shrimp (Penaeus indicus), Uka Shrimp (Metapenaeus brevicornis), Spur Shrimp (Palaemon styliferus), Ube Shrimp (Metapenaeus tenuipes) and Swallow Shrimp (Metapenaeus affinis). From the research that has been done, the data obtained that the speciesof shrimp in Nipah Panjang are more than the speciesof shrimp in Mendahra Ilir Village. One of the factors that cause differences in catches is the type of fishing gear used.

Involvement of nerve cord-expressed SVWC2 in pathogen recognition and defense in Macrobrachium nipponense.

In this study, we identified two β-hexosaminidase A isoforms (Mn-HexA1 and Mn-HexA2) in Macrobrachium nipponense through bioinformatics analysis and characterized their phylogenetic relationships. The open reading frames of Mn-HexA1 and Mn-HexA2 were 1641 bp (encoding 546 amino acids) and 1473 bp (encoding 490 amino acids), respectively. Both isoforms exhibited high conservation, sharing five identical functional domains, with 58.43% amino acid sequence similarity. Quantitative PCR analysis revealed that Mn-HexA1 expression was significantly higher than Mn-HexA2 across all developmental stages and tissues. During embryonic development, Mn-HexA1 showed elevated expression at the ZS, L15, and PL10, while Mn-HexA2 was upregulated only at L15 and PL10. In the breeding season and non-breeding season, Mn-HexA1 and Mn-HexA2 were predominantly expressed in the hepatopancreas at levels significantly higher than in other tissues. Moreover, their expression in most tissues was higher during the breeding season than in the non-breeding season. RNA interference experiments revealed that knockdown of both Mn-HexA isoforms significantly accelerated ovarian development in M. nipponense, with the Mn-HexA1-silenced group exhibiting faster progression than the Mn-HexA2-silenced group. These results demonstrate that Mn-HexA genes function as negative regulators of ovarian maturation, with Mn-HexA1 exerting a stronger inhibitory effect than Mn-HexA2.

The effect of different dietary canthaxanthin levels on water quality parameters, growth performance, biochemical indices, immunological responses, body composition, and metabolic profiles of oriental river prawn (Macrobrachium nipponense)