Lysosomal Acid Β-glucosidase Research Articles

Acid hydrolases in neuronal and glial enriched fractions of rat brain

Fractions enriched in neuronal and glial cells from rat brain were assayed for several lysosomal acid hydrolases, namely, β-galactosidase (β-D-galactoside galactohydrolase, EC 3.2.1.23), β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21), α-galactosidase (α-D-galactoside galactohydrolase, EC 3.2.1.22), α-mannosidase (α-D-mannoside mannohydrolase, EC 3.2.1.24), N-acetyl-β-glucosaminidase (β-2-acetamido-2-deoxy-D-glucoside acetamidodeoxyglucohydrolase, EC 3.2.1.30), N-acetyl-β-galactosaminidase, acid phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.2), β-glucuronidase (β-D-glucuronide glucuronohydrolase, EC 3.2.1.31), arylsulfatase (aryl-sulfate sulfohydrolase, EC 3.1.6.1), glucocerebrosidase and galactocerebrosidase. The data do not show any significant difference in the distribution of these activities between the two cell types.