Lysimeter Study Research Articles

Magnetite-coated biochar as a soil phosphate filter: From laboratory to field lysimeter

Agriculture is one of the largest anthropogenic contributors of phosphorus (P) to surface waters and reducing these P loads is a target set by many countries. One potential mitigation strategy could be to utilize biochar, functionalized by co-precipitating metal ions onto its surface to adsorb phosphates. We evaluated such an approach in a series of laboratory experiments and a three-year field lysimeter study. Leached phosphates were trapped in a 3-cm layer of a commercially available 20% hardwood/80% Norway spruce biochar, coated with magnetite and placed under the soil root zone. Langmuir maximum adsorption potential (Qmax) was 3.38 mg P g−1 biochar, as determined from batch adsorption studies performed at pH 6.5. Further laboratory experiments revealed that adsorption was strongly negatively correlated with pH (R2 = 0.995). In a laboratory column study using high flow rates, no difference was found in phosphate adsorption between coated biochar and the control (no biochar), after application of ammonium phosphate at a rate corresponding to 22 kg P ha−1. However, differences were observed at higher application rates (285 or 570 kg P ha−1). Calculation of a Fe:P molar ratio to evaluate the magnetite treatment method from laboratory (2:1) to lysimeter (248:1) suggested a relatively effective laboratory performance, but overall poor field efficiency. Nevertheless, biochar in the lysimeter study still reduced phosphate leaching from two organic soils, by 62% (P < 0.05) and 35% (NS). This suggests that, even though the method of magnetite-coating biochar is not necessarily resource-efficient, it is effective in removing P from soil leachate.

Nitrate leaching losses are lower from ryegrass/white clover forages containing plantain than from ryegrass/white clover forages under different irrigation

ABSTRACTThe aim of this research was to assess the potential of using perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) forages containing plantain (Plantago lanceolata L.) (PRGWC + Plantain) to mitigate nitrate (NO3−) leaching in grazed grassland. Lysimeter studies were carried out to determine NO3− leaching losses from the PRGWC + Plantain forage, compared with the standard perennial ryegrass and white clover forage (PRGWC) under three different irrigation conditions (flood, rotorainer and pivot). Fresh dairy cow urine (700 kg N ha−1) was applied to these lysimeters in early summer (December) and late summer (February). Nitrogen (N) dynamics and the abundance of ammonia oxidising bacteria (AOB) and archaea (AOA) were also determined in companion mini-plots alongside the lysimeters. Nitrate leaching losses from the PRGWC + Plantain forage were 82% lower than those from the PRGWC forage when urine was applied in December and 74% less when urine was applied in February (P < .05). This was possibly due to the release of biological nitrification inhibitors by plantain which slowed down the nitrification process and this, combined with a lower drainage volume, was largely responsible for the lower NO3− leaching losses in the PRGWC + Plantain forages. The three different irrigation schemes did not significantly affect NO3− leaching losses under the two forage types. These results demonstrate the potential of using PRGWC + Plantain forages as an effective mitigation option for NO3− leaching in grazed grasslands.

Italian ryegrass swards reduce N leaching via greater N uptake and lower drainage over perennial ryegrass cultivars varying in cool season growth rates

ABSTRACTPasture mitigation strategies are needed to reduce the negative effects of nitrate (NO3−) leaching from intensive pastoral livestock production systems. We explored the capability of pasture grasses with different cool season (winter–early spring) growth rates to reduce NO3− leaching and increase N uptake. Following a single urine application of 700 kg N ha−1, NO3− leaching loss and N uptake by Italian ryegrass and four perennial ryegrass cultivars of varying seasonal growth (heading dates from –7 to +20 days), either undersown with/without Italian ryegrass, were quantified in a 12 month lysimeter study. Italian ryegrass cv. Tabu had lower NO3−–N leaching loss and drainage, and greater N uptake and DM yield than perennial ryegrass varieties. Italian ryegrass total N leaching loss (143 kg N ha−1 yr−1) was 46–33% less (P < .05) than early and mid-season maturing Tyson, Arrow and AberDart perennial ryegrass. Italian ryegrass total N uptake (463 kg N ha−1) was 1.2–1.4 times greater than all other grass sward types. Overall, perennial ryegrass pure swards had similar N uptake and N leaching. Late-season maturing One 50 perennial ryegrass showed significantly reduced (P < .05) total N leaching loss. Undersowing Italian ryegrass into established perennial ryegrass swards resulted in lower NO3− leaching and greater N uptake than non-undersown perennial ryegrass swards, though not significant. Results confirm Italian ryegrass is a useful pasture grass option for reducing annual N leaching loss. Cool season perennial ryegrass varieties appear not to influence soil N uptake and leaching loss.

Plant available phosphorus in soil as predictor for the leaching potential: Insights from long-term lysimeter studies

This study aimed to demonstrate the impact of phosphorus (P) mineral fertilization on topsoil P content and P leaching. We evaluated 83 datasets from 25 years from lysimeter experiments involving different cropping systems (winter crop, summer crop and autumn tillage, harvested grass) or unfertilized fallow, four types of soil texture, and three levels of applied mineral P fertilizer. A positive monotonic and significant correlation was indicated between P in the topsoil determined by the double lactate method (PDL) and the yearly flow-weight total (TP) concentrations in leachates with Spearman rank correlations rs (rs > 0.183) and probability (p) < 0.05. The present German recommended rates of P mineral fertilization are proposed insufficient to protect fresh and marine waters from undesired P pollution and eutrophication. A long-term reduction of excess soil P is urgent along with other measures to mitigate high P inputs to surface and ground waters.

Comparison of nitrate leaching from oats and Italian ryegrass catch crops following simulated winter forage grazing: a field lysimeter study

ABSTRACTIntensive winter forage grazing systems have a high risk of environmental impact due to nitrate leaching from urine deposition on cool, wet, fallow soils. We investigated the effect of sowing two catch crops after simulated winter forage grazing on kale to potentially reduce N leaching losses from a winter application of labelled 15N-urine (350 and 700 kg N ha−1) using field lysimeters. The experiment measured the capture of N by the sowing at recommended dates of either oats (Avena sativa) or Italian (It.) ryegrass (Lolium multiflorum) and the resulting 15N balance. Results showed that the sowing of an oats catch crop reduced nitrate leaching by 25% over the critical winter–spring drainage period compared with It. ryegrass. Relatively small amounts of the labelled 1⁵N-urine (3–4%) were captured in the catch crops of urine treatments so the reduced nitrate leaching under the oats crop was attributed to lower drainage over the period.

Cropping System and Type of Pig Manure Affect Nitrate-Nitrogen Leaching in a Sandy Loam Soil.

The application of livestock manure can result in the loss of nitrate-nitrogen (NO-N) and degrade surface and groundwater. A 3-yr lysimeter study was set up to compare the effect of cropping system and nitrogen (N)- and phosphorus (P)-based pig manure application rates on the loss of water and NO-N below the root zone. The experiment was a split-plot design with annual and perennial cropping systems as the main factor. Five nutrient management treatments were the subplots: N-based liquid pig manure application; P-based liquid pig manure application, N-based solid pig manure application, P-based solid pig manure application, and a control without amendment. The results showed that 40 to 60 kg NO-N ha was lost from the annual plots in 2010 and 23 to 60 kg NO-N ha in 2011, whereas a negligible amount of NO-N was lost from the perennial (<1 kg ha) plots in both years. The application of solid pig manure on a P basis followed by urea in subsequent years reduced the risk of NO-N leaching over the course of the rotation, likely due to immobilization of N by the straw in the solid pig manure. Our study showed that a perennial cropping system consisting of a mixture of grasses has the capacity to receive and utilize significant amounts of nutrients with negligible amount of nutrient leakages to the adjacent environment. The inclusion of grasses in a crop rotation and their use to take up excess nutrients are sustainable practices that will benefit the environment.

Ecotoxicity and fate of a silver nanomaterial in an outdoor lysimeter study

Sewage sludge is repeatedly applied as fertilizer on farmland due to its high nutrient content. This may lead to a significant increase of silver nanomaterials (AgNM) in soil over years. Therefore, our aim was to investigate the ecotoxicity and fate of AgNM under environmentally relevant conditions in outdoor lysimeters over 25 months. Two AgNM concentrations (1.7 and 8.0 mg/kg dry matter soil) were applied via sewage sludge into soil. In subsamples of the soil, incubated under laboratory conditions for 180 days, the comparability of outdoor and laboratory results regarding ecotoxicity was determined. The results from our long term lysimeter experiments show no detectable horizontal displacement in combination with very low remobilization to the percolate water. Thus, indicate that the sludge applied AgNM remains nearly immobile in the pathway between soils and leachate. However, Ag uptake to the roots of wheat and canola suggests that the chemical conditions in the rhizosphere induce AgNM remobilization from the incorporated sewage sludge even after two harvesting cycles. At the higher AgNM concentration a steady inhibition of the soil microflora was observed over 25 month in the lysimeter study, while there was no effect at the lower AgNM concentration. The results of the laboratory experiment reflect the findings of the lysimeter study and indicate that a risk assessment for AgNM based on data from laboratory tests is acceptable.

15N recoveries from ruminant urine patches on three forage types

15Nitrogen balances are useful tools to increase our understanding of soil N processes and the efficiency of plants in taking up animal urine-N. This study aimed to investigate the effect of forage type on the fate of autumn-applied urine-N (700 kg N ha−1). In a 17-month lysimeter study the recoveries of 15N in herbage, leachate, nitrous oxide, and soil + roots (at the end of the trial) were determined for three forage types: perennial ryegrass-white clover (RGWC, Lolium perenne L.-Trifolium repens L.), Italian ryegrass (Italian RG, Lolium multiflorum Lam.), and lucerne (Medicago sativa L.) under simulated autumn grazing. We recovered 81.5–85.5% of the 15N applied in the urine. Italian RG had the greatest amount of urine-15N recovered in the herbage (49.5%) and the least recovered in leachate (16.8%) making it the most efficient of the three forages for utilizing urine-N. For RGWC, herbage and leachate recoveries were 40.1% and 23.7%, respectively. Lucerne was the least efficient with recoveries of 15N in herbage and leachate equaling 18.4% and 52.5%, respectively. This study demonstrates that Italian ryegrass could offer benefits in reducing urine-N leaching losses through its ability to take up more urine-N over winter.

Modeling the water and nitrogen transports in a soil–paddy–atmosphere system using HYDRUS-1D and lysimeter experiment

Efficient water and fertilizer use is of paramount importance both in rain-fed and irrigated rice cultivation systems to tread off between the crop water demand during the dry spell and the fertilizer leaching. This lysimeter study on paddy in a lateritic sandy loam soil of the eastern India, to simulate the water and solute transports using the HYDRUS-1D model, reveals that this model could very well simulate the soil depth-specific variations of water pressure heads and nitrogen (N) concentrations with the efficiency of >86 and 89%, respectively. The change in the level of water ponding depth did not have a significant effect on the time to peak and the temporal variability of N concentration in the bottom soil layer. The lysimeter-scale water balance analysis indicated that the average deep percolation loss and crop water use were 35.01 ± 2.03 and 39.74 ± 1.49% of the total water applied during the crop growth period, respectively. Similarly, the amount of N stored in the plant and lost through soil storage, deep percolation, and other losses (mineralization, denitrification, and gaseous N loss to the atmosphere through plant leaves) were 1.60 ± 0.16, 0.17 ± 0.04, 12.00 ± 0.48, and 86.23 ± 0.41% of the total applied nitrogen, respectively. The simulation results reveal that a constant ponding depth of 3 cm could be maintained in paddy fields to reduce the N leaching loss to 7.5 kgN/ha.

An Empirical Calibration for Heat‐Balance Sap‐Flow Sensors in Maize

Core Ideas Sap flow errors with heat‐balance sap‐flow sensors are quantified. An empirical equation is established for correcting heat‐balance sap‐flow measurements in maize. Independent tests proved the usefulness of the calibration equation in maize. Sap flow measurements with heat‐balance sap‐flow (HBSF) sensors are subject to errors due to temperature heterogeneity across the plant stem. Here we develop and evaluate an empirical calibration for HBSF sensors to measure transpiration rates (T) of maize (Zea mays L.). A pot experiment was used to establish an empirical calibration equation relating T determined by a mass balance method and sap flow velocity (V) measured with HBSF sensors. The calibration equation was tested in a field weighing lysimeter study, a pot study from the literature, and an additional dataset where V was measured with HBSF sensors, and T was determined from independent measurements of evapotranspiration and evaporation. In all studies, HBSF sensor measured V overestimated T, and the errors displayed diurnal dynamics: small in the evening and early morning, became larger with increasing T, and reached a maximum when solar irradiance was the largest. A linear calibration equation, T’ = 0.65V + 0.39, was established to convert measured V (g plant−1 h−1) values to corrected transpiration rates T’ (g plant−1 h−1). Using this equation, the largest sap flow error was reduced by 60, 50, and 50% in the lysimeter experiment, pot experiment, and field study, respectively.

Nitrate Leaching from Winter Cereal Cover Crops Using Undisturbed Soil‐Column Lysimeters

Cover crops are important management practices for reducing nitrogen (N) leaching, especially in the Chesapeake Bay watershed, which is under total maximum daily load (TMDL) restraints. Winter cereals are common cool-season crops in the Bay watershed, but studies have not directly compared nitrate-N (NO-N) leaching losses from these species. A 3-yr cover crop lysimeter study was conducted in Beltsville, MD, to directly compare NO-N leaching from a commonly grown cultivar of barley ( L.), rye ( L.), and wheat ( L.), along with a no-cover control, using eight tension-drained undisturbed soil column lysimeters in a completely randomized design with two replicates. The lysimeters were configured to exclude runoff and to estimate NO-N leaching and flow-weighted NO-N concentration (FWNC). The temporal pattern of NO-N leaching showed a consistent highly significant ( < 0.001) effect of lower NO-N leaching with cover crops compared with no cover but showed only small and periodically significant ( < 0.05) effects among the cultivars of barley, rye, and wheat covers. Nitrate-N leaching was more affected by the quantity of establishment-season (mid-October to mid-December) precipitation than by cover crop species. For example, compared with no cover, winter cereal covers reduced NO-N leaching 95% in a dry year and 50% in wet years, with corresponding reductions in FWNC of 92 and 43%, respectively. These results are important for scientists, nutrient managers, and policymakers because they directly compare NO-N leaching from winter cereal covers and expand knowledge for developing management practices for winter cereals that can improve water quality and increase N efficiency in cropping systems.

Contaminants Leaching from Fresh Poultry Waste: A Lysimeter Study on Sandy Soils under Tropical Conditions

A lysimeter study was conducted on sandy soil of Minna, North central Nigeria to assess the migration of nitrates, phosphates, bacteriological parameters and heavy metals contents of poultry manure through Minna soil. This is with a view to examining the environmental effect of indiscriminate land application of excessive poultry manure on shallow groundwater quality. A lysimeter and rainfall simulator assembly was installed on an undisturbed 0.9m diameter, 3m depth soil core after which 50kg of characterized poultry manure was applied at the top. Rainfall of 125mm was simulated and water samples were collected at different depths of the soil core through the lysimeter and were taken to laboratory for analysis. Results showed that nitrate and phosphate in poultry manure were able to leach to a depth of 2.5m of the soil core four months after poultry manure application while turbidity and electrical conductivity reached their maximum value at depth 2.5m after three months. Faecal coliform, total coliform and faecal streptococci were detected at depth 2.5m also two months after the application. Statistical analysis using New Duncan Multiple Range test showed significant variation (p&lt;0.05) of all the parameters tested with depth of sample collection and months after application of poultry manure. Spearman’s correlation coefficient established both positive and negative correlation between the parameters studied in this research. Heavy metals tested, Arsenic, copper, zinc, chromium and manganese were not able to leach beyond 0.5m depth of the soil core throughout the experimental period.

Effect of application of dairy manure, effluent and inorganic fertilizer on nitrogen leaching in clayey fluvo-aquic soil: A lysimeter study

Dairy farm manure and effluent are applied to cropland in China to provide a source of plant nutrients, but there are concerns over its effect on nitrogen (N) leaching loss and groundwater quality. To investigate the effects of land application of dairy manure and effluent on potential N leaching loss, two lysimeter trials were set up in clayey fluvo-aquic soil in a winter wheat-summer maize rotation cropping system on the North China Plain. The solid dairy manure trial included control without N fertilization (CK), inorganic N fertilizer (SNPK), and fresh (RAW) and composted (COM) dairy manure. The liquid dairy effluent trial consisted of control without N fertilization (CF), inorganic N fertilizer (ENPK), and fresh (FDE) and stored (SDE) dairy effluent. The N application rate was 225kgNha−1 for inorganic N fertilizer, dairy manure, and effluent treatments in both seasons. Annual N leaching loss (ANLL) was highest in SNPK (53.02 and 16.21kgNha−1 in 2013/2014 and 2014/2015, respectively), which were 1.65- and 2.04-fold that of COM, and 1.59- and 1.26-fold that of RAW. In the effluent trial (2014/2015), ANLL for ENPK and SDE (16.22 and 16.86kgNha−1, respectively) were significantly higher than CF and FDE (6.3 and 13.21kgNha−1, respectively). NO3− contributed the most (34–92%) to total N leaching loss among all treatments, followed by dissolved organic N (14–57%). COM showed the lowest N leaching loss due to a reduction in NO3− loss. Yield-scaled N leaching in COM (0.35kgNMg−1 silage) was significantly (P<0.05) lower than that in the other fertilization treatments. Therefore, the use of composted dairy manure should be increased and that of inorganic fertilizer decreased to reduce N leaching loss while ensuring high crop yield in the North China Plain.

Tracking effluent discharges in undisturbed stony soil and alluvial gravel aquifer using synthetic DNA tracers

With the intensification of human activities, fresh water resources are increasingly being exposed to contamination from effluent disposal to land. Thus, there is a greater need to identify the sources and pathways of water contamination to enable the development of better mitigation strategies. To track discharges of domestic effluent into soil and groundwater, 10 synthetic double-stranded DNA (dsDNA)33Double-stranded DNA (dsDNA); single-stranded DNA (ssDNA); base-pair (bp); bromide (Br); polymerase chain reaction (PCR); colony-forming units (cfu); relative mass recovery (RB); relative transport speed (RV); relative dispersion (RS); concentration breakthrough curve (BTC). tracers were developed in this study. Laboratory column experiment and field groundwater and soil lysimeter studies were carried out spiking DNA with oxidation-pond domestic effluent. The selected DNA tracers were compared with a non-reactive bromide (Br) tracer with respect to their relative mass recoveries, speeds of travel and dispersions using the method of temporal moments. In intact stony soil and gravel aquifer media, the dsDNA tracers typically showed earlier breakthrough and less dispersion than the Br tracer, and underwent mass reduction. This suggests that the dsDNA tracers were predominantly transported through the network of larger pores or preferential flow paths. Effluent tracking experiments in soil and groundwater demonstrated that the dsDNA tracers were readily detectable in effluent-contaminated soil and groundwater using quantitative polymerase chain reaction. DNA tracer spiked in the effluent at quantities of 36μg was detected in groundwater 37m down-gradient at a concentration 3-orders of magnitude above the detection limit. It is anticipated it could be detected at far greater distances. Our findings suggest that synthetic dsDNA tracers are promising for tracking effluent discharges in soils and groundwater but further studies are needed to investigate DNA-effluent interaction and the impact of subsurface environmental conditions on DNA attenuation. With further validation, synthetic dsDNA tracers, especially when multiple DNA tracers are used concurrently, can be an effective new tool to track effluent discharge in soils and groundwater, providing spatial estimation on the presence or absence of contamination sources and pathways.

Long-term monitoring of sulfonamide leaching from manure amended soil into groundwater

Veterinary antibiotics such as sulfonamides are frequently applied in livestock farming worldwide. Due to poor absorption in the animal gut and/or reversible metabolization sulfonamides are excreted in considerable amounts and can subsequently be detected in liquid manure. As manure is utilized for soil fertilization, sulfonamides can enter the environment via this pathway. Water samples taken below an agriculture field in Lower Saxony revealed the permanent entrance of sulfamethazine into groundwater and concentrations up to 100 ng L−1 were determined. During a long-term lysimeter study, nine sulfonamides were applied to two different soil types by using fortified liquid manure. Divert mobilities were found with sulfamethazine und sulfamethoxazole showing the highest detection frequency in water samples taken below both bedrock and sandy soil. Four years after the last application of fortified manure, sulfonamides were still detectable in the leachate. Based on analyses of manure and fermentation residue samples, a permanent input of sulfonamides to the soil can be excluded. Thus, the positive findings must be caused by the antibiotics once applied. Soils fertilized with manure contaminated with sulfonamides can consequently be a long-time source for the transfer of antibiotics into groundwater.

Ground-penetrating radar (GPR) responses for sub-surface salt contamination and solid waste: modeling and controlled lysimeter studies.

The assessment of polluted areas and municipal solid waste (MSW) sites using non-destructive geophysical methods is timely and much needed in the field of environmental monitoring and management. The objectives of this study are (i) to evaluate the ground-penetrating radar (GPR) wave responses as a result of different electrical conductivity (EC) in groundwater and (ii) to conduct MSW stratification using a controlled lysimeter and modeling approach. A GPR wave simulation was carried out using GprMax2D software, and the field test was done on two lysimeters that were filled with sand (Lysimeter-1) and MSW (Lysimeter-2). A Pulse EKKO-Pro GPR system with 200- and 500-MHz center frequency antennae was used to collect GPR field data. Amplitudes of GPR-reflected waves (sub-surface reflectors and water table) were studied under different EC levels injected to the water table. Modeling results revealed that the signal strength of the reflected wave decreases with increasing EC levels and the disappearance of the subsurface reflection and wave amplitude reaching zero at higher EC levels (when EC >0.28S/m). Further, when the EC level was high, the plume thickness did not have a significant effect on the amplitude of the reflected wave. However, it was also found that reflected signal strength decreases with increasing plume thickness at a given EC level. 2D GPR profile images under wet conditions showed stratification of the waste layers and relative thickness, but it was difficult to resolve the waste layers under dry conditions. These results show that the GPR as a non-destructive method with a relatively larger sample volume can be used to identify highly polluted areas with inorganic contaminants in groundwater and waste stratification. The current methods of MSW dumpsite investigation are tedious, destructive, time consuming, costly, and provide only point-scale measurements. However, further research is needed to verify the results under heterogeneous aquifer conditions and complex dumpsite conditions.

A large-scale lysimeter study of stormwater biofiltration under cold climatic conditions

Stormwater biofiltration was studied at a large-scale lysimeter facility in southern Finland. Biofiltration systems, constructed within eight lysimeters, consisted of an organic topsoil, a sand layer and three gravel layers of varying gravel size underneath. Six of the lysimeter systems were vegetated with Phalaris arundinacea, Salix glauca var. callicarpaea and Lythrum salicaria, and two systems were left unplanted. The effect of vegetation on the functioning of the systems was studied by comparing the vegetated with the non-vegetated systems. The systems were irrigated during three seasons with artificial stormwater containing nitrate, phosphate, zinc, copper and aluminium to study pollutant retention and water infiltration during warm and cold temperatures. Half of the systems also received road salt (NaCl) to study its effect on the functioning of the systems. Phosphate, zinc and copper were well retained (81–98%) during each season in each system. Nitrate retention improved (from 0 to 47%) with time in the vegetated system but negative retention was also observed during some irrigation events, indicating that nitrate also leached from the parent soil. The retention of aluminium in the unsalted biofilters decreased (from ca. 80 to 0%) during the snowmelt season in spring and was highly negative in the salted systems during spring and summer. Salt also slightly diminished (ca. 8%) the retention of Cu. Vegetation only improved the retention of nitrate. However, the non-vegetated systems infiltrated water poorly while the vegetated systems infiltrated large volumes and intensities of water one year after biofilter establishment. Hence, vegetation was necessary for operational infiltration. The study showed positive results for stormwater biofiltration in cold climates. Nonetheless, long-term studies with varying soil types and pollutants are recommended.

Giant reed growth and effects on soil biological fertility in assisted phytoremediation of an industrial polluted soil

Phytoremediation is a cost-effective “green technology” that uses plants to improve the soil properties of polluted sites, preventing the dispersion of pollutants and reducing the mobility of potentially toxic elements (PTEs) through their adsorption and accumulation by roots or precipitation within the root zone. Being highly tolerant to pollutants and other abiotic stresses, giant reed (Arundo donax L.) is a suitable biomass crop for phytoremediation of contaminated soils.We report the results of a two-year open-air lysimeter study aimed at assessing the adaptability of giant reed to grow on industrial substrates polluted by Pb and Zn and at testing commercial humic acids from leonardite as improvers of plant performance. We evaluated giant reed potential for: 1) biomass production for energy or biomaterial recovery; 2) PTE phytoextraction and 3) soil fertility restoration. Chemical fertility was monitored by measuring soil C while soil biological fertility was estimated by quantifying the abundance of bacterial functional genes regulating nitrogen fixation (nifH) and nitrification (amoA).Giant reed above-ground growth on the polluted soils was slightly lower (−16%) than on a non-polluted soil, with a preferential storage of biomass in the rhizome acting as a survival strategy in limiting growing conditions. Humic acids improved plant stress tolerance and production levels. As aerial biomass (shoots) did not accumulate PTEs, the plant in question can be used for bioenergy or biopolymer production. In contrast, below-ground biomass (rhizomes) accumulated PTEs, and can thus be harvested and removed from soil to improve phytoremediation protocols and also used as industrial biofuel. Giant reed growth increased the abundance of N-cycling bacteria and soil C in the rhizospheric soil, as well as reduced soil Pb and Zn EDTA extractable fraction.

Effects of forage type and gibberellic acid on nitrate leaching losses

AbstractNitrogen (N) leaching from soil into water is a significant concern for intensively grazed forage‐based systems because it can cause a decline in water quality and is a risk to human health. Urine patches from grazing animals are the main source of this N. The objective of this study was to quantify the effect that forage type and gibberellic acid (GA) application had on N leaching and herbage N uptake from urine patches on perennial ryegrass–white clover (RGWC), Italian ryegrass and lucerne. A lysimeter study was conducted over 17 months to measure herbage growth, N uptake and N loss to water beneath each of the three forage types with the following treatments: control, urine (700 kg N/ha) and urine with GA (8 g GA active ingredient/ha). Compared with RGWC (205 kg N/ha), N leaching losses were 35.3% lower from Italian ryegrass (133 kg N/ha) and 98.5% higher from lucerne (407 kg N/ha). These differences in leaching loss are likely to be due to winter plant growth and N uptake. During the winter months, Italian ryegrass had higher N uptake, whereas lucerne had lower N uptake, compared with RGWC. The application of GA had no effect on N leaching losses, DM yield or N uptake of forage treated with 700 kg N/ha urine.

Effect of Temperature and Plow Pan on Water Movement in Monolithic Weighable Lysimeter with Paddy Sandy Loam Soil during Winter Season

The monolithic weighing lysimeter is a useful facility that could directly measure water movement via layers, drainage, and evapotranspiration (ET) with precise sensors. We evaluated water movement through layers and water balance using the lysimeter with undisturbed paddy sandy loam soil, Gangseo soil series (mesic family of Anthraquic Eutrudepts classified by Soil Taxonomy) during winter season from Dec. 2014 to Feb. 2015. Daily ET indicated up to 1.5 mm in December and January and 2 mm in February. The abrupt increase of soil water tension at the depth of 0.1 m, when soil temperature at the same depth was below <TEX>$2^{\circ}C$</TEX>, was observed due to temporary frost heaving. The surface evaporation was less than reference ET below -15 kPa of soil water potential at the depth of 0.1 m. The maximum drainage rate was similar to the saturated hydraulic conductivity of a plow pan layer. Both upward and downward water movement, related to ET and drainage, were retarded by a plow pan layer. This study demonstrated that the lysimeter study could well quantify water balance components even under frost heaving during winter season and that a plow pan with low permeability could act as a boundary that affects drainage and evapotranspiration.