Luminance Response Research Articles

Direct measurements of luminal Ca 2+ with endo-lysosomal GFP-aequorin reveal functional IP 3 receptors.

Endo-lysosomes are considered acidic Ca 2+ stores but direct measurements of luminal Ca 2+ within them are limited. Here we report that the Ca 2+ -sensitive luminescent protein aequorin does not reconstitute with its cofactor at highly acidic pH but that a significant fraction of the probe is functional within a mildly acidic compartment when targeted to the endo-lysosomal system. We leveraged this probe (ELGA) to report Ca 2+ dynamics in this compartment. We show that Ca 2+ uptake is ATP-dependent and sensitive to blockers of endoplasmic reticulum Ca 2+ pumps. We find that the Ca 2+ mobilizing messenger IP 3 which typically targets the endoplasmic reticulum evokes robust luminal responses in wild type cells, but not in IP 3 receptor knock-out cells. Responses were comparable to those evoked by activation of the endo-lysosomal ion channel TRPML1. Stimulation with IP 3 -forming agonists also mobilized the store in intact cells. Super-resolution microscopy analysis confirmed the presence of IP 3 receptors within the endo-lysosomal system, both in live and fixed cells. Our data reveal a physiologically-relevant, IP 3 -sensitive store of Ca 2+ within the endo-lysosomal system.

Method for determination of the spectral mismatch correction factors for the luminous responsivity of photometers when measuring the characteristics of LED lamps

This paper describes the challenges associated with the metrology of LED sources. The main problem is the difference between the spectra of LED lamps and the spectrums of reference lamps, which are mainly highly stable and well-reproducible incandescent lamps. The difference in spectra leads to the fact that the luminous responsivity of the calibrated photometer will be different for the reference incandescent lamp and for the LED lamp. The difference is caused by the fact that a relative spectral characteristic of the photometer responsivity is always different from the spectral responsivity of the human eye (V(λ) curve), which determines all luminous quantities. The greater the difference between the relative spectral responsivity of the photometer and V(λ) is, the more the luminous responsivity of the photometer will differ for the reference lamp and for the LED lamp. The paper describes a method developed at the NSC “Institute of Metrology” for experimental determination of mismatch correction factors of luminous responsivity that account for the difference in the LED spectra to solve the problems of metrological support of LED sources. Methods of experimental study of mismatch correction factors for measuring instruments of luminous quantities of LED radiation sources are presented.

Evaluation of anti-vector immune responses to adenovirus-mediated lung gene therapy and modulation by αCD20

Although the last decade has seen tremendous progress in drugs that treat cystic fibrosis (CF) due to mutations that lead to protein misfolding, there are approximately 8%-10% of subjects with mutations that result in no significant CFTR protein expression demonstrating the need for gene editing or gene replacement with inhaled mRNA or vector-based approaches. A limitation for vector-based approaches is the formation of neutralizing humoral responses. Given that αCD20 has been used to manage post-transplant lymphoproliferative disease in CF subjects with lung transplants, we studied the ability of αCD20 to module both T and B cell responses in the lung to one of the most immunogenic vectors, E1-deleted adenovirus serotype 5. We found that αCD20 significantly blocked luminal antibody responses and efficiently permitted re-dosing. αCD20 had more limited impact on the Tcell compartment, but reduced tissue resident memory Tcell responses in bronchoalveolar lavage fluid. Taken together, these pre-clinical studies suggest that αCD20 could be re-purposed for lung gene therapy protocols to permit re-dosing.

Effect of eccentric fixation on the steady-state pattern electroretinogram

PurposeThe steady-state pattern electroretinogram (ssPERG) is used to assess retinal ganglion cell function in a variety of research contexts and diagnostic applications. In certain groups of patients or study participants, stable central fixation of the stimulus is not guaranteed. The present study aimed at assessing the effects of misfixation on the ssPERG response to checkerboard reversal stimuli.MethodsUsing two check sizes (0.8° and 15°), we compared ssPERG responses for several amounts of fixation deviation, ranging from 0° to 19° horizontally and from 0° to 14° diagonally. The stimulus area extended to 15° eccentricity, stimulus reversal rate was 15/s.ResultsUp to around 7° eccentricity, there was no sizable effect of fixation deviation under most conditions. Effects were somewhat larger for nasal than for temporal deviation, in particular for small checks. Diagonal deviation was associated with a response to luminance onset/offset at 7.5 Hz (subharmonic of the reversal rate), most prominently when the interior of a large check was fixated.ConclusionGenerally, moderate inaccuracies of fixation do not have a sizable effect on ssPERG amplitude. However, with large checks, the luminance response has to be considered.

Cone-Driven, Geniculocortical Responses in Canine Models of Outer Retinal Disease.

Canine models of inherited retinal degeneration are used for proof of concept of emerging gene and cell-based therapies that aim to produce functional restoration of cone-mediated vision. We examined functional magnetic resonance imaging (MRI) measures of the postretinal response to cone-directed stimulation in wild-type (WT) dogs, and in three different retinal disease models. Temporal spectral modulation of a uniform field of light around a photopic background was used to target the canine L/M (hereafter "L") and S cones and rods. Stimuli were designed to separately target the postreceptoral luminance (L+S) and chrominance (L-S) pathways, the rods, and all photoreceptors jointly (light flux). These stimuli were presented to WT, and mutant PDE6B-RCD1, RPGR-XLPRA2, and NPHP5-CRD2 dogs during pupillometry and functional MRI (fMRI). Pupil responses in WT dogs to light flux, L+S, and rod-directed stimuli were consistent with responses being driven by cone signals alone. For WT animals, both luminance and chromatic (L-S) stimuli evoked fMRI responses in the lateral geniculate nucleus or visual cortex; RCD1 animals with predominant rod loss had similar responses. Responses to cone-directed stimulation were reduced in XLPRA2 and absent in CRD2. NPHP5 gene augmentation restored the cortical response to luminance stimulation in a CRD2 animal. Cone-directed stimulation during fMRI can be used to measure the integrity of luminance and chrominance responses in the dog visual system. The NPHP5-CRD2 model is appealing for studies of recovered cone function. fMRI assessment of cone-driven cortical response provides a tool to translate cell/gene therapies for vision restoration.

Junction temperature and luminous flux prediction for white LED array based on electrical-photo-thermal modeling

During the operation of an LED array, its thermal and optical performances are always not equal to the superposition of the individual LED's characteristics because of a significant thermal coupling effect between the arrays. Based on this, this paper proposes an electrical–photo-thermal model, with considering both junction temperature and luminous flux, to predict the both the thermal and optical performances of LED arrays operated under different currents, case temperatures, and lighting methods. The junction temperature and luminous flux of a single LED operating under different driving currents and case temperature conditions are firstly collected to establish the luminous flux response surface model of a single chip. Then it is used to predict the luminous flux of an array, whose junction temperature is predicted using both thermal coupling matrix (TCM) and numerical models. Experiments verify the luminous flux of the LED array under different operation conditions and show that the proposed electrical–photo-thermal modeling can be used to predict the thermal and optical parameters of LED arrays with 95 % accuracy. Thus, it is effective for the fast prediction of the junction temperature and luminous flux of large LED systems with array structures, i.e. intelligent automotive lightings and displays.

Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment

Lineage transitions are a central feature of prostate development, tumourigenesis and treatment resistance. While epigenetic changes are well known to drive prostate lineage transitions, it remains unclear how upstream metabolic signalling contributes to the regulation of prostate epithelial identity. To fill this gap, we developed an approach to perform metabolomics on primary prostate epithelial cells. Using this approach, we discovered that the basal and luminal cells of the prostate exhibit distinct metabolomes and nutrient utilization patterns. Furthermore, basal-to-luminal differentiation is accompanied by increased pyruvate oxidation. We establish the mitochondrial pyruvate carrier and subsequent lactate accumulation as regulators of prostate luminal identity. Inhibition of the mitochondrial pyruvate carrier or supplementation with exogenous lactate results in large-scale chromatin remodelling, influencing both lineage-specific transcription factors and response to antiandrogen treatment. These results establish reciprocal regulation of metabolism and prostate epithelial lineage identity.

Biocompatible, Europium-Doped Fluorapatite Nanoparticles as a Wide-Range pH Sensor.

The development of a simple, biocompatible, pH sensor with a wide range of detection, using a single fluorescent probe is highly important in the medical field for the early detection of diseases related to the pH change of tissues and body fluids. For this purpose, europium-doped fluorapatite (FAP: Eu) nanoparticles were synthesized using the coprecipitation method. Doping with the rare earth element europium (Eu) makes the non-luminescent phosphate mineral fluorapatite, luminescent. The luminous response of the sample upon dissolution in hydrochloric acid (HCl), in highly acidic to weakly basic media, makes it a potential pH sensor. A linear variation was observed with an increase in pH, in both the total intensity of emission and the R-value or the asymmetry ratio. The ratiometric pH sensing enabled by the variation in R-value makes the sensor independent of external factors. The structural, optical, and photoluminescent (PL) lifetime analysis suggests a particle size-dependent pH sensing mechanism with the changes in the coordinated water molecules around the Eu3+ ion in the nanoparticle. Given its exceptional biocompatibility and pH-dependent fluorescence intensity for a wide range of pH from 0.83 to 8.97, the probe can be used as a potential candidate for pH sensing of biological fluid.

Evaluation of electroretinography (ERG) parameters as a biomarker for ADHD

BackgroundThe retina is recognized as an accessible part of the brain due to their common embryonic origin. The electroretinogram (ERG) has proven to be a valuable tool for detecting schizophrenia and bipolarity. We therefore investigated its ability to detect ADHD. MethodsThe cone and rod luminance response functions of the ERG were recorded in 26 ADHD subjects (17 women and 9 men) and 25 controls (16 women and 9 men). ResultsNo significant differences were found between the mixed groups, but sexual dysmorphia was observed in the significant results. In males, a significant prolonged cone a-wave latency was observed in the ADHD group. In females, we observed a significant decrease in the cone a- and b-wave amplitudes and a trend for a prolonged cone b-wave latency as well as a higher scotopic mixed rod-cone a-wave in the ADHD group. ConclusionThe data obtained in this study show the potential of the ERG to detect ADHD, warranting further large-scale studies.

Method for characterizing small-spot luminance in medical virtual reality headsets

We present an experimental method that utilizes a conic probe attached to an integrating sphere for characterizing the small-spot luminance of virtual reality (VR) head-mounted displays (HMDs). This allows for the measurement of relative luminance in virtual scenes and the assessment of VR displays for visualizing grayscale medical images. We characterized the relative luminance of the VIVE Pro and Oculus Rift by displaying test patterns with a circular dark spot of varying diameter in the bright field and compared the probe performance with commercially available systems. Compared to a conventional flat-panel display, the results show a significant level of veiling glare in the HMDs using both a customized conic probe with improved optical performance and an advanced imaging photometer. We also found no significant change in the relative luminance response with the probe’s working distance for the HMDs due to their unique optical architecture for near-eye viewing conditions. Our results highlight the issue of high veiling glare in HMDs and the importance of small-spot luminance measurements to adequately evaluate the technical performance of HMDs for emerging medical applications.

Nationwide survey on the current situation of quality control of diagnostic displays in Japan

The Japan Association of Radiological Technologists (JART) and the Japan Medical Imaging and Radiological Systems Industries Association jointly conducted a nationwide survey to reveal the current situation of diagnostic displays in Japan using a questionnaire on the performance and quality control (QC) of diagnostic displays for mammography and common use. The questionnaire for radiological technologists (RTs) was distributed via email to 4519 medical facilities throughout Japan, where RTs affiliated with JART were employed; 613 (13.6%) facilities responded. Diagnostic displays with suitable maximal luminance (500cd/m2 or higher for mammography and 350cd/m2 or higher for common use) and resolution (5 megapixels for mammography) have been widely used. However, while 99% of the facilities recognized the necessity of QC, only approximately 60% implemented it. This situation arose due to several barriers to QC implementation, such as insufficient devices, time, staff, knowledge, and the recognition of QC as a duty. The implementation of QC can lead to the avoidance of incidents or accidents caused by a decrease in luminance, variation in luminance response, and the influence of ambient light. Moreover, the barriers discouraging the implementation of QC are mainly related to a lack of human resources and budgets. Therefore, to popularize the QC of diagnostic displays in all facilities, it is crucial to identify countermeasures to eliminate these barriers and to continue positive actions for popularization.

Immune checkpoint inhibitors in luminal gastrointestinal malignancies: going beyond MSI-H/dMMR, TMBand PD-L1.

In luminal gastrointestinal tumors, immune checkpoint inhibitors (ICIs) targeting PD-1, PD-L1and CTLA-4 have been investigated in multiple settings. The indications for these drugs are primarily dependent on specific biomarkers that imply immunogenicity: overexpression of PD-L1, tumor mutational burden, loss of mismatch repair proteins (dMMR) and/or highmicrosatellite instabilitystatus. Although these markers can be both predictive and prognostic, there is variability in how they are measured and used to guide therapies. Moreover, the use of ICIs can be further refined with a better understanding of the tumor microenvironment and interactions with other available therapies. The purpose of this review is to characterize luminal gastrointestinal tumors'responses to ICIs considering known predictive biomarkers and discuss emerging therapeutic approaches using ICIs.

Electroluminescent strain sensing on carbon fiber reinforced polymer

The present paper demonstrates an inexpensive and less labor-intensive machine vision-based strain and damage visualization technique compared to traditional carbon fiber reinforced polymer material inspection methods. This technique is used to characterize the fracture and failure properties upon progressive axial loading in textile carbon fiber reinforced polymer (CFRP) lamina with non-intrusive smart electroluminescent (EL) integration. The smart EL structure utilizes CFRP's unique conductive and piezoresistive properties to visualize deformation and cracks through brightness change. Two fiber orientations (±45° and 0°/90°) of twill-weave CFRP lamina are fabricated according to a modified ASTM D3039 for linear and fatigue loading in uniaxial mechanical tests to analyze both fiber and matrix-induced EL responses. The EL-strain visualization method is validated with standardized digital image correlation (DIC) utilizing the natural speckling pattern of the EL CFRP lamina. The axial EL-strain results for both static and cyclic loading highly agree with the strain extracted planar conventional DIC strain field on standard and damaged CFRP samples in terms of spatial resolution and sensitivity. This novel strain and damage visualization method aims to be a portable, low-cost, and real-time alternative to non-destructive testing methods commonly used in the composite industry. Instead of periodic checkups, EL architecture allows for real-time strain and damage visualization of composite structure. • Fabrication and tensile testing of strain sensing EL illuminating woven CFRP in [±45°] and [0°/90°] fiber orientations. • Visual damage and strain detection are achieved with EL CFRP structure. • Planar DIC strain fields produced on EL illuminating and conventional airbrushed patterns show strong correspondence. • Variations in piezoresistive and luminance response exhibit correlation with strain.

18‐2: Assessment of Image Quality in Augmented Reality Displays Using a Computational Model of Target Detectability

We characterized augmented reality (AR) displays and developed a computational task‐based model to evaluate their image quality. The model enables fast assessment of image quality and evaluation of tradeoffs between display configurations such as display luminance response, uniformity, resolution, and noise. This work supports the development and optimization of AR displays without extensive measurements or resource‐intensive perception studies.

Abstract PD2-04: Molecular plasticity of luminal breast cancer and response to CDK 4/6 inhibition - The biomarker program of the PENELOPE-B trial investigating post-neoadjuvant palbociclib

Abstract Background: Molecular plasticity of breast cancer is crucial for the development of therapy-resistant disease. In this investigation, we studied changes in molecular signatures between pretherapeutic (pre-Tx) and post-therapeutic (post-NACT) tumor samples from patients included in the PENELOPE-B (NCT01864746) trial. The phase III PENELOPE-B study did not show a significant benefit from palbociclib in women with centrally confirmed HR+, HER2- primary breast cancer without a pathological complete response after taxane-containing neoadjuvant chemotherapy (NACT) and at high-risk of relapse (CPS-EG score ≥3 or 2 and ypN+) (Loibl et al. JCO 2021). However, first translational investigations showed that a small number of patients with a luminal-B tumor subtype, based on absolute intrinsic molecular subtyping (AIMS, Paquet & Hallet, JNCI 2014) subtyping after NACT, had a numerical benefit from post-NACT palbociclib. We have therefore extended the analysis and included a cohort of paired pre-Tx and post-NACT samples. Methods: We investigated gene expression in pre-Tx (n=259) tumor tissue samples using the HTG EdgeSeq Oncology Biomarker Panel including 2549 genes (HTG Molecular Diagnostics Inc.); for the same patients the same panel on post-NACT residual tumor samples were available. The paired samples were selected based on a case-cohort approach. Based on 91 genes of this panel, the AIMS subtype was calculated. In addition, we performed exploratory biomarker analyses to identify genes and gene signatures with prognostic and predictive relevance. After completion of NACT, PENELOPE-B patients were randomized to palbociclib versus placebo in addition to standard endocrine therapy. Results: The prevalence of AIMS subtypes, in particular LumA vs LumB, changed in pre-Tx and post-NACT tumors. In the pre-Tx samples, 115 (44%) and 123 (47%) of tumors had LumA and LumB subtypes, respectively, as expected from a high-risk cohort. However, in the post-NACT samples, LumA tumors were predominant (n=183, 71%) over LumB (n=30; 12%). 78 (30%) and 6 (2%) tumors switched their subtype from LumB to LumA and LumA to LumB, respectively. For further analyses, we compared the groups of low proliferating (LumA and NormL) and high proliferating subtypes (LumB, BasalL and HER2E). In bivariable Cox regression analysis, the grouped pre-Tx and post-NACT AIMS subtypes were independent prognostic factors for iDFS: HR=1.85 (1.16-2.98, p=0.011) for pre-Tx LumB/BasalL/HER2E vs LumA/NormL and HR=2.18 (1.24-3.84, p=0.007) for post-NACT. Similar results were found when adjusted for prognostic clinical factors and for DDFS and OS endpoints although the pre-Tx subtype did not reach significance. These and further Cox models investigating interaction effects show that patients with tumors developing from high (pre-Tx) to low proliferation (post-NACT) had a higher iDFS risk compared to stable low proliferating tumors but a lower iDFS risk compared to stable high proliferating tumors. Neither in the pre-Tx LumB/BasalL/HER2E nor in the pre-Tx LumA/NormL subgroup a benefit from palbociclib was observed. Based on the results of the AIMS subtyping, we extended the exploratory analysis to identify genes that might be involved in the prognostic and predictive effects as well as genes driving the subtype switch. The analysis is ongoing and the relevant genes will be presented at the conference. Conclusions: Our findings show that the switch from high-risk molecular subtypes (in particular LumB) to low-risk subtypes (in particular LumA) is common in neoadjuvant therapy of luminal tumors. The adaptation of luminal high-risk tumors to therapy-induced stress is crucial for the clinical outcome and the results suggest that molecular defined tumor subtypes might not be as stable as originally thought. Citation Format: Carsten Denkert, Frederik Marmé, Miguel Martin, Michael Untch, Hervé Bonnefoi, Sung-Bae Kim, Harry Bear, Agnieszka Witkiewicz, Seock-Ah Im, Angela DeMichele, Laura Van’t Veer, Nicole McCarthy, Thorsten Stiewe, Karen A. Gelmon, José A. García-Sáenz, Catherine M. Kelly, Toralf Reimer, Erik Knudsen, Nicholas Turner, Federico Rojo, Peter A. Fasching, Julia Teply-Szymanski, Yuan Liu, Masakazu Toi, Hope S. Rugo, Michael Gnant, Andreas Makris, Bärbel Felder, Karsten Weber, Sibylle Loibl. Molecular plasticity of luminal breast cancer and response to CDK 4/6 inhibition - The biomarker program of the PENELOPE-B trial investigating post-neoadjuvant palbociclib [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr PD2-04.

Calibration of a modular trap detector system towards a new realisation of the luminous intensity unit

A modular photometric trap detector system has recently been developed at Physikalisch-Technische Bundesanstalt (PTB). All parts of the detector are now completely calibrated. The new planned traceability chain for the realisation of luminous intensity unit can therefore be established for the first time. This contribution shows the results of the individual calibration steps including the associated measurement uncertainties and correlations. A major part of the calibrations along the traceability chain is done at the upgraded measurement setup TULIP (TUnable Lasers In Photometry). The improvements of the TULIP setup are presented and the effects on the measurement uncertainty are shown. The result of the first complete calibration according to the new traceability chain is compared to previous calibration results both in terms of spectral irradiance responsivity and luminous responsivity. The further steps required towards implementing the new traceability chain and the possible implications are discussed.

Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish.

Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer retina and modulate light decrement detection through an ATP/pH-dependent mechanisms and adenosine/dopamine signaling. Here, we decipher how the strategic localization of Panx1b channels in the inner retina and ganglion cell layer modulates visually evoked motor behavior. We describe a panx1b knockout model generated by TALEN technology. The RNA-seq analysis of 6 days post-fertilization larvae is confirmed by real-time PCR and paired with testing of locomotion behaviors by visual motor and optomotor response tests. We show that the loss of Panx1b channels disrupts the retinal response to an abrupt loss of illumination and it decreases the larval ability to follow leftward direction of locomotion in low light conditions. We concluded that the loss of Panx1b channels compromises the final output of luminance as well as motion detection. The Panx1b protein also emerges as a modulator of the circadian clock system. The disruption of the circadian clock system in mutants suggests that Panx1b could participate in non-image forming processes in the inner retina.

1758O Neoadjuvant pembrolizumab in localized/locally advanced solid tumors with mismatch repair deficiency

Pembrolizumab (pembro) significantly improves clinical outcomes in advanced/metastatic microsatellite instability high (MSI-H)/deficient mismatch repair (dMMR) solid tumors. This study evaluates pembro in the neoadjuvant space with the potential for an organ-sparing approach. This is a phase 2 open-label, single center trial of MSI-H/dMMR non-metastatic solid tumors with localized unresectable or high risk resectable (defined as ≥ 20% recurrence) with measurable disease. Treatment is Pembro 200mg every 3 wks for 8 cycles followed by surgical resection with option to continue therapy for 18 cycles followed by observation. First restaging is at 6 wks and includes baseline and 3-week 70-gene ctDNA assessment. To continue on study, patients are required to have PR/CR, SD with tumor shrinkage or SD with decline in ctDNA. The co-primary endpoints are safety and pathological complete response (pCR). Key secondary endpoints are response rate and organ-sparing at one year for patients who declined surgery. Between 10/2019 and 3/2021, 35 pts were enrolled and study has completed enrollment. Tumor types included 27 CRC and 8 non-CRC: endometrial, gastric, meningioma, 2 duodenal, ampullary and 2 pancreatic. Median follow-up was 9 months (range 0.1 - 17). Among 32 evaluable pts, best ORR was 75% (CR 25% , PR 50%), SD 22% and PD 3%. Luminal endoscopic response was seen in 17/19 (89%) pts. Among 8 (23%) pts who underwent surgery, pCR was seen in 4. At present non-operative approach was chosen by 8 (23%) pts with 1-year organ-sparing seen in 2/2 evaluable pts. Treatment-related grade 3/4 immune adverse events were seen in 3 (9%) pts (transaminitis, diarrhea and type 1 diabetes). Baseline ctDNA mutations were present in 20 (57%) pts and at 3 weeks declined in 15 (75%), unchanged in 1, no paired 3 week sample in 1, and increased in 3. Tumor microenvironment immune analysis is ongoing and will be presented. Neoadjuvant pembrolizumab in dMMR/MSI-H cancers is safe and resulted in high rates of radiographic and endoscopic response which has implications for organ-sparing strategies. Non-operative management of dMMR/MSI-H localized solid tumors should be further investigated.

Abstract NG05: Epigenetic mechanisms of endocrine therapy response in breast cancer

Abstract NG05: Epigenetic mechanisms of endocrine therapy response in breast cancer

Effects of pupillary light and darkness reflex on the generation of pro- And anti-saccades.

Saccades are often directed toward a stimulus that provides useful information for observers to navigate the visual world. The quality of visual signals of a stimulus is influenced by global luminance, and the pupil constricts or dilates after a luminance increase or decrease, respectively, to optimize visual signals for further information processing. Although luminance level changes regularly in the real environment, saccades are mostly studied in the luminance-unchanged setup. Whether pupillary responses triggered by global luminance changes modulate saccadic behavior are yet to be explored. Through varying background luminance level in an interleaved pro- and anti-saccade paradigm, we investigated the modulation of pupillary luminance responses on the generation of reflexive and voluntary saccades. Subjects were instructed to either automatically look at the peripheral stimulus (pro-saccade) or to suppress the automatic response and voluntarily look in the opposite direction from the stimulus (anti-saccade). Level of background luminance was increased (light), decreased (dark), or unchanged (control) during the instructed fixation period. Saccade reaction time distributions of correct pro- and anti-saccades in the light and dark conditions were differed significantly from those in the control condition. Moreover, the luminance condition modulated saccade kinematics, showing reduced performances in the light condition than in the control condition, particularly in pro-saccades. Modeling results further suggested that both pupil diameter and pupil size derivative significantly modulated saccade behavior, though effect sizes were small and mainly mediated by intersubject differences. Together, our results demonstrated the influence of pupillary luminance responses on the generation of pro- and anti-saccades.