Low Milk Research Articles

Genetic analysis of phenotypic indicators for heat tolerance in crossbred dairy cattle

Climate change-induced rise in global temperatures has intensified heat stress on dairy cattle and is contributing to the generally observed low milk productivity. Selective breeding aimed at enhancing animals’ ability to withstand rising temperatures while maintaining optimal performance is crucial for ensuring future access to dairy products. However, phenotypic indicators of heat tolerance are yet to be effectively factored into the objectives of most selective breeding programs. This study investigated the response of milk production to changing heat load as an indication of heat tolerance and the influence of calving season on this response in multibreed dairy cattle performing in three agroecological zones Kenya. First-parity 7-day average milk yield (65 261 milk records) of 1 739 cows were analyzed. Based on routinely recorded weather data that were accessible online, the Temperature-Humidity Index (THI) was calculated and used as a measure of heat load. THI measurements used represented averages of the same 7-day periods corresponding to each 7-day average milk record. Random regression models, including reaction norm functions, were fitted to derive two resilience indicators: slope of the reaction norm (Slope) and its absolute value (Absolute), reflecting changes in milk yield in response to the varying heat loads (THI 50 and THI 80). The genetic parameters of these indicators were estimated, and their associations with average test-day milk yield were examined. There were no substantial differences in the pattern of milk yield response to heat load between cows calving in dry and wet seasons. Animals with ≤50% Bos taurus genes were the most thermotolerant at extremely high heat load levels. Animals performing in semi-arid environments exhibited the highest heat tolerance capacity. Heritability estimates for these indicators ranged from 0.06 to 0.33 and were mostly significantly different from zero (P < 0.05). Slope at THI 80 had high (0.64–0.71) negative correlations with average daily milk yield, revealing that high-producing cows are more vulnerable to heat stress and vice versa. A high (0.63–0.74) positive correlation was observed between Absolute and average milk yield at THI 80. This implied that low milk-producing cows have a more stable milk production under heat-stress conditions and vice versa. The study demonstrated that the slope of the reaction norms and its absolute value can effectively measure the resilience of crossbred dairy cattle to varying heat load conditions. The implications of these findings are valuable in improving the heat tolerance of livestock species through genetic selection.

Lactation induction in a transgender woman: case report and recommendations for clinical practice

BackgroundWe present a case of non-puerperal induced lactation in transgender woman. Medical literature on lactation induction for transgender women is scarce, and the majority of literature and protocols on lactation induction is based on research in cisgender women. Healthcare professionals may lack the precise knowledge about lactation induction and may therefore feel insecure when advice is requested. Subsequently, there is a rising demand for guidelines and support.MethodsPatient medical record was consulted and a semi-structured interview was conducted to explore the motive for lactation induction, the experience of lactation induction, and to gather additional information about the timeline and course of events.Case presentationIn this case a 37-year-old transgender woman, who was under the care of the centre of expertise on gender dysphoria in Amsterdam, and in 2020 started lactation induction because she had the wish to breastfeed her future infant. She was in a relationship with a cisgender woman and had been using gender affirming hormone therapy for 13 years. Prior to initiating gender affirming hormone therapy she had cryopreserved her semen. Her partner conceived through Intracytoplasmic Sperm Injection, using our patient’s cryopreserved sperm.To induce lactation, we implemented a hormone-regimen to mimic pregnancy, using estradiol and progesterone, and a galactogogue; domperidone. Our patient started pumping during treatment. Dosage of progesterone and estradiol were significantly decreased approximately one month before childbirth to mimic delivery and pumping was increased. Our patient started lactating and although the production of milk was low, it was sufficient for supplementary feeding and a positive experience for our patient.Two weeks after birth, lactation induction was discontinued due to suckling problems of the infant and low milk production.ConclusionsThis case report underlined that lactation induction protocols commonly used for cisgender women are also effective in transgender women. However, the amount of milk produced may not be sufficient for exclusive nursing. Nevertheless, success of induced lactation may be attributed to its importance for parent-infant bonding, rather than the possibility of exclusive chestfeeding.

The Relation between Milk Lactose Concentration and the Rumination, Feeding, and Locomotion Behavior of Early-Lactation Dairy Cows.

This study hypothesizes that higher in-line milk lactose concentrations are indicative of enhanced dairy cow behaviors-including increased rumination, feeding, and locomotion activities-reflecting superior overall health and well-being. It posits that fluctuations in milk lactose levels have a substantial impact on the physiological and behavioral responses of dairy cows, thereby affecting their milk yields and compositions. Each cow's milk lactose, fat, protein, and fat-to-protein ratio were continuously monitored using the BROLIS HerdLine in-line milk analyzer (Brolis Sensor Technology, Vilnius, Lithuania). The RumiWatch noseband sensor (RWS; ITIN + HOCH GmbH, Fütterungstechnik, Liestal, Switzerland) was employed to measure the biomarkers of the rumination, feeding, and locomotion behavior. The measurements were recorded over 5 days at the same time (during morning milking). A total of 502 cows were examined. During these 5 days, 2510 measurements were taken. Based on the lactose content in their milk, the cows were divided into two categories: the first group consisted of cows with milk lactose levels below 4.70%, while the second group included cows with milk lactose levels of 4.70% or higher. Our study showed that cows with higher milk lactose concentrations (≥4.70%) produced significantly more milk (16.14% increase) but had a lower milk protein concentration (5.05% decrease) compared to cows with lower lactose levels. These cows also exhibited changes in rumination and feeding behaviors, as recorded by the RWS: there was an increase in the mastication and rumination behaviors, evidenced by a 14.09% rise in other chews and a 13.84% increase in rumination chews, along with a 16.70% boost in bolus activity. However, there was a notable 16.18% reduction in their physical activity, as measured by the change in time spent walking.

110 Effects of sire selection on puberty and reproduction of offspring selected as replacement heifers

Abstract Sire selection is important to maintain the desired genetic capability of any cattle herd; therefore, selection of economic production traits affect profitability by increasing pre- and post-weaning gain and carcass quality potential. Using heifers developed from sires selected for growth and milk traits may have long-term impact on sustainability of the cowherd by influencing mature cow size and milk production. Our objective was to determine the impacts of sires selected for high and moderate growth and high and low milk traits on growth and reproductive performance of replacement heifers. Therefore, we selected replacement heifers sired by bulls within the upper 5th and 50th percentiles of the Angus breed for yearling weight (YW) EPD as high growth (HG) moderate growth (MG), the upper 15th percentile of the Angus breed for milk EPD as high milk (HM), and the lower 15th percentile for low milk (LM) in a 2×2 factorial design resulting in 4 treatments: HG/HM, HG/LM, MG/HM, and MG/LM. Heifers (n = 46) from appropriate AI sires that were born and developed at Oklahoma State University – Range Cow Research Center – South Range Unit were selected as replacements. They were raised on a base diet of dry, dormant tall grass prairie and moderate quality grass hay following weaning and 2 kg/d of 16% crude protein concentrate supplement. Body weights and biometrics were recorded at weaning and approximately at 1-yr of age, and blood was collected at 11 and 0 d before synchronization for progesterone analysis to determine pubertal status pre-breeding. Estrus was synchronized using the 14-d CIDR-PG with timed AI method followed by a 45-d natural service breeding period. Pregnancy checks were conducted by serum collection 35-d following AI and via rectal palpation by an experienced veterinarian 112 d after removal of the bulls. Statistical analyses were performed using the mixed procedure of SAS (SAS 9.4, SAS INC., Carey, NC). Heifers from high growth sires were 28 kg heavier at weaning (P &amp;lt; 0.01) and 26 kg heavier as yearlings (P &amp;lt; 0.01) than those sired by moderate growth sires, while sire milk EPD affected neither (P ≥ 0.55). High growth and high milk sired heifers had greater hip heights, frame scores and thus greater (P ≤ 0.05) estimated mature body weight (EMBW). Despite this, sire selection had no impact on percentage of heifers pubertal before breeding, AI pregnancy, or the total pregnancy rates (P &amp;gt; 0.24) Based on these data, selection of sires for HG and HM leads to increased productivity of the offspring but gives rise to larger mature cows, which may pressure grazing resources in limited resource environments impacting the long-term sustainability of the cow-calf enterprise.

Using of Nanoparticles in treating of Hydatid Disease in Domestic Animals

The parasite Echinococcus granulosus is the cause of unilocular hydatid disease, which is a serious health risk to people and domestic animals worldwide. Livestock with hydatid disease suffers substantial financial losses due to the slaughterhouse's disapproval of the diseased animal parts, productivity losses (such as lowered live weight gain, milk yield, reproductive rates, and hide and skin value), and expenses related to caring for both humans and animals. Because of the parasite's complex life cycle and the difficulties associated with traditional treatment techniques, new strategies are needed to handle this crippling illness more successfully. In the treatment of hydatid illness, nanomedicine and nanoparticles have shown great promise, providing new approaches to medication distribution, focused therapy, diagnosis, and control measures. The possible roles and applications of nanomedicine and nanoparticles in treating hydatid illness in domestic animals are reviewed in this article. Owing to their distinct physicochemical characteristics at the nanoscale, nanoparticles enable tailored medication administration, enhancing anthelmintic agent potency while reducing systemic side effects. Therapeutic drugs like praziquantel or albendazole can be encapsulated in these nanoparticles, allowing for improved permeability and retention at the location of the parasite cysts. Additionally, imaging agents and diagnostic instruments at the nanoscale enable. Additionally, nanotechnology offers avenues for developing innovative control measures, including environmental disinfection and targeted delivery of parasiticides. Collaborative efforts between researchers, veterinarians, and experts in nanotechnology are crucial to harnessing the full potential of nanoparticles and nanomedicine in effectively managing the infection in domestic animals.

Mother's Emotional Experiences of Breastfeeding with Primary Low Milk Supply in the First Four Months Postpartum: An Interpretative Phenomenological Analysis.

Background: Primary low milk supply (PLMS) prevents mothers from producing sufficient milk to breastfeed exclusively. However, limited evidence exists regarding women's experiences of breastfeeding with PLMS. Objective: This article aims to investigate the emotional experiences of mothers breastfeeding with PLMS in the first 3 months postpartum. Materials and Methods: The study was conducted in Ireland and used a phenomenological methodology to investigate the lived experiences of breastfeeding mothers with PLMS. Nine first-time breastfeeding mothers with PLMS participated, and data collection took the form of unstructured interviews. Data analysis was completed using Interpretative Phenomenological Analysis. Results: Being in the Whirlwind is one of four superordinate themes identified in this study. This theme relates to participants' internalized experiences of breastfeeding with PLMS in the first 3 months postpartum. During this time, participants struggled to come to terms with having PLMS and became caught up in all-consuming efforts to increase their milk supply. They experienced guilt, sadness, confusion, anger, and anxiety, with many describing the early months postpartum as traumatic. Participants revealed how triple-feeding (a regime of breastfeeding, pumping, and supplementing) negatively affected their mental health and reported that supplementing with infant formula was emotionally upsetting. Conclusions: Our findings reveal that the combination of PLMS and triple feeding can negatively impact a mothers' mental health. A greater understanding among health care professionals of the emotional impact of having PLMS and triple-feeding could enhance the provision of sensitive and person-centered support for those with PLMS. Antenatal breastfeeding education should acknowledge that PLMS is a challenge for a small cohort of women and place greater emphasis on the emotional aspects of breastfeeding challenges.

Breast hypoplasia markers among women who report insufficient milk production: A retrospective online survey.

To estimate the proportions of anatomical breast characteristics suggestive of breast hypoplasia among breastfeeding women self-reporting low milk supply. We also explored breast hypoplasia risk factors. Online survey conducted between October 2021 and January 2022. Five low milk supply Facebook groups. 487 women reporting low milk supply with their first child born ≥ 37 weeks gestation within 5 years of participation in this study, and residing in the USA, Australia or the UK. We present data on the primary outcome ('breast type') for 399 women. Women were excluded if the dyad was separated for more than 24 hours during the hospital stay, or if the mother reported removing milk less than 6 times per day from each breast on most days before being aware of having insufficient milk production. The proportions of proposed breast hypoplasia markers including atypical breast type, widely spaced breasts, breast asymmetry, stretch marks on the breast and lack of pregnancy breast growth. We also estimated the odds of having breast hypoplasia markers in at-risk groups compared to reference groups, adjusting for covariates. Approximately 68% reported at least one atypical breast (270/399; 95% CI: 62.9%, 72.1%). Around 47% reported widely spaced breasts (212/449; 95% CI: 42.7%, 52.7%), 72% a lack of pregnancy breast growth (322/449; 95% CI: 68.3%, 77.4%), and 76% stretch marks on the breast (191/250; 95% CI: 70.7%, 81.3%). Multiple logistic regression analyses identified being overweight during pubertal years as a risk factor for atypical breast type and lack of pregnancy breast growth. Participants in low milk supply Facebook groups reported high rates of breast hypoplasia markers. Being overweight during adolescence was a risk factor for breast hypoplasia markers. These findings should be confirmed in well-conducted large cohort studies to determine the strongest combination of hypoplasia markers in predicting low supply.

Optimizing dietary rumen-degradable starch to rumen-degradable protein ratio improves lactation performance and nitrogen utilization efficiency in mid-lactating Holstein dairy cows.

The dietary rumen-degradable starch (RDS) to rumen-degradable protein (RDP) ratio, denoted as the RDS-to-RDP ratio (SPR), has been proven to enhance in vitro rumen fermentation. However, the effects of dietary SPR in vivo remain largely unexplored. This study was conducted to investigate the effect of dietary SPR on lactation performance, nutrient digestibility, rumen fermentation patterns, blood indicators, and nitrogen (N) partitioning in mid-lactating Holstein cows. Seventy-two Holstein dairy cows were randomly assigned to three groups (24 head/group), balanced for (mean ± standard deviation) days in milk (116 ± 21.5), parity (2.1 ± 0.8), milk production (42 ± 2.1 kg/d), and body weight (705 ± 52.5 kg). The cows were fed diets with low (2.1, control), medium (2.3), or high (2.5) SPR, formulated to be isoenergetic, isonitrogenous, and iso-starch. The study consisted of a one-week adaptation phase followed by an eight-week experimental period. The results indicated that the high SPR group had a lower dry matter intake compared to the other groups (p < 0.05). A quadratic increase in milk yield and feed efficiency was observed with increasing dietary SPR (p < 0.05), peaking in the medium SPR group. The medium SPR group exhibited a lower milk somatic cell count and a higher blood total antioxidant capacity compared to other groups (p < 0.05). With increasing dietary SPR, there was a quadratic improvement (p < 0.05) in the total tract apparent digestibility of crude protein, ether extract, starch, neutral detergent fiber, and acid detergent fiber. Although no treatment effect was observed in rumen pH, the rumen total volatile fatty acids concentration and microbial crude protein synthesis increased quadratically (p < 0.05) as dietary SPR increased. The molar proportion of propionate linearly increased (p = 0.01), while branched-chain volatile fatty acids linearly decreased (p = 0.01) with increasing dietary SPR. The low SPR group (control) exhibited higher concentration of milk urea N, rumen ammonia N, and blood urea N than other groups (p < 0.05). Despite a linear decrease (p < 0.05) in the proportion of urinary N to N intake, increasing dietary SPR led to a quadratic increase (p = 0.01) in N utilization efficiency and a quadratic decrease (p < 0.05) in the proportion of fecal N to N intake. In conclusion, optimizing dietary SPR has the potential to enhance lactation performance and N utilization efficiency. Based on our findings, a medium dietary SPR (with SPR = 2.3) is recommended for mid-lactating Holstein dairy cows. Nevertheless, further research on rumen microbial composition and metabolites is warranted to elucidate the underlying mechanisms of the observed effects.

Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels

Milk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized these cows to unravel molecular pathways governing post-absorptive body N pools focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic urea formation and mammary gland N excretion. While we observed minor adjustments in cellular energy metabolism in different tissues associated with different MU levels, no transcriptional differences in liver ammonia detoxification were detected, despite significant differences in MU between the groups. Differential expression of AQP3 and SLC38A2 in the kidney provides evidence for higher urea concentration in the collecting duct of LMU cows than HMU cows. The mammary gland exhibited the most significant differences, particularly in tricarboxylic acid (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. These findings suggest that selecting for lower MU could lead to altered urinary urea (UU) handling and changes in milk protein synthesis. However, given the genetic variability in N metabolism components, the long-term effectiveness of MU-based selection in reducing N emissions remains uncertain.

Monitoring the Use of Human Milk, the Ideal Food for Very Low-Birth-Weight Infants-A Narrative Review.

Aware of the utmost importance of feeding premature babies-especially those of lower weight-with human milk, as well as the need to monitor this important element of neonatal care, we focused on four aspects in this review. First of all, we reviewed the beneficial effects of feeding premature infants with breast milk in the short and long term. Secondly, we performed a quantitative evaluation of the rates of breastfeeding and feeding with human milk in Very-Low-Birth-Weight infants (VLBWs) during hospitalization in the Neonatal Intensive Care Unit (NICU) and at discharge. Our aim was to take a snapshot of the current status of human milk-feeding care and track its trends over time. Then we analyzed, on the one hand, factors that have been proven to facilitate the use of maternal milk and, on the other hand, the risk factors of not feeding with breast milk. We also considered the spread of human milk banking so as to assess the availability of donated milk for the most vulnerable category of premature babies. Finally, we proposed a protocol designed as a tool for the systematic monitoring of actions that could be planned and implemented in NICUs in order to achieve the goal of feeding even more VLBWs with human milk.

Associations of lameness with milk composition, fatty acid profile, and milk coagulation properties in mid-lactation high-yielding Holstein cows

Lameness, an animal welfare and profitability related problem in dairy industry, was evaluated on 126 multiparous Estonian Holstein cows on a 5-point scale; score three or above indicated lameness. Milk samples of the cows were analysed for basic composition, content of mineral elements, fatty acid (FA) profile and coagulation properties (MCP). Lame cows had lower milk yield and a lower milk protein and milk fat yield compared to non-lame cows. MCP and content of mineral elements were not affected by lameness. The concentrations of most individual milk FA, their groups formed by chain length, degree of saturation or by origin were not affected by the lameness. Lameness was associated with a higher concentration of odd- and branched-chain fatty acids and C18:0. Milk from lame cows is suitable for consumers as lameness did not impair nutritional value nor MCP of the milk.

Use of At-Breast Supplementers to Facilitate Feeding Solely at the Breast for Infants with Retrognathia: Two Case Studies.

Objective: Infants with significant retrognathia often have difficulty forming a latch adequate to establish exclusive breastfeeding. This article describes the use of at-breast supplementers (ABSs) to facilitate extended breastfeeding relationships, even when supplementation is necessary for growth. Methods: Two cases are described where infants with severe retrognathia initially struggled with weight gain necessitating supplementation but were able to ultimately exclusively feed at-breast with the use of ABSs. Results: While the two cases differed in the form of supplemental milk used and duration of ABS use, both dyads breastfed for beyond 2 years. Conclusions: Feeding solely at the breast and subsequent extended breastfeeding may be possible even for infants who require supplementation, including those with anatomical or functional challenges such as retrognathia. The ABS is a relatively simple system that may be beneficial for difficulties such as poor latch and low milk supply. More awareness and education is needed so that clinicians consider supporting dyads with this approach.

Short-chain fatty acids in breast milk and their relationship with the infant gut microbiota.

The short-chain fatty acids (SCFAs) contained in breast milk play a key role in infant growth, affecting metabolism and enhancing intestinal immunity by regulating inflammation. In order to examine the associations between the microbiota and SCFA levels in breast milk, and explore the roles of SCFAs in regulating the infant gut microbiota, we enrolled 50 paired mothers and infants and collected both breast milk and infant fecal samples. Breast milk SCFA contents were determined by UPLC-MS, and whole genome shotgun sequencing was applied to determine the microbial composition of breast milk and infant feces. The SCFA levels in breast milk were grouped into tertiles as high, medium, or low, and the differences of intestinal microbiota and KEGG pathways were compared among groups. The results demonstrated that breast milk butyric acid (C4) is significantly associated with Clostridium leptum richness in breastmilk. Additionally, the specific Bifidobacterium may have an interactive symbiosis with the main species of C4-producing bacteria in human milk. Women with a low breast milk C4 tertile are associated with a high abundance of Salmonella and Salmonella enterica in their infants' feces. KEGG pathway analysis further showed that the content of C4 in breast milk is significantly correlated with the infants' metabolic pathways of lysine and arginine biosynthesis. This study suggests that interactive symbiosis of the microbiota exists in breast milk. Certain breast milk microbes could be beneficial by producing C4 and further influence the abundance of certain gut microbes in infants, playing an important role in early immune and metabolic development.

“I didn’t want to let go of the dream”: Exploring women’s personal stories of how their low milk supply was discovered

ProblemLow milk supply is the most common reason women give for stopping breastfeeding early and yet there is a lack of understanding about these women’s experiences. BackgroundMost women plan to breastfeed but many experience challenges such as low milk production, leading them to seek help and support. AimTo explore women’s personal stories of how their low supply was discovered. MethodsInductive template analysis was used to analyse free-text online survey responses of women from the United States of America, Australia and the United Kingdom. Findings384 women responded to the open-ended survey item between October 2021 and January 2022. We identified three themes: (i) Events and observations: From ‘risk factors’ to ‘failure of breast changes’ to ‘my baby was so unhappy’, (ii) Seeking support and taking action: ‘I tried everything’ and (iii) A rollercoaster of emotion: ‘I didn’t want to let go of the dream’. DiscussionOur findings emphasise women’s need to feel heard and understood and their quest to find answers. The rollercoaster of emotions they experienced largely stemmed from a gap between the expectations and reality of breastfeeding. Some participants described accepting a different feeding journey. ConclusionFindings underscore the need for quality and accessible psychosocial support for women experiencing low milk supply, in addition to the provision of evidence-based advice.

Stress related to wild canid predators near dairy sheep farms associated with increased somatic cell counts in bulk-tank milk

We investigated the association between wild canid predators reported near sheep farms throughout Greece and somatic cell counts in bulk-tank milk as a reflection of milk quality. The study included 325 dairy sheep flocks, where bulk-tank milk somatic cell counts and total bacterial counts were measured and staphylococci were isolated. Farms were divided into three groups: Cohort A (farms with no reports of wild canid predators nearby), B (farms with canid predators (golden jackal and grey wolf) nearby yet with no experience of livestock losses to predation) and C (farms with canid predators nearby and livestock losses to predation). Somatic cell counts in bulk-tank milk of Cohort C farms were significantly higher, + 43% and + 29%, compared to those for Cohorts A and B, respectively: 0.617 × 106 cells mL−1 versus 0.433 × 106 or 0.477 × 106 cells mL−1, respectively. The presence of wild canid predators near sheep farms was associated with lower quality milk potentially indicative of stress consistent with the potential effects of a landscape of fear. Increasing biosecurity measures at livestock farms, e.g., fencing, and presence of livestock guard dogs could minimise predation risk, whilst also improving livestock welfare by reducing predator-associated stress.

A Survey of U.S. and Canadian Physicians’ Clinical Practices Related to Pharmacologic, Herbal, and Nutritional Substances Used to Increase Milk Production

Objectives:Physicians may be asked to prescribe, recommend, or discuss foods, herbs, and medications for low milk supply. It is unclear how factors such as education, experience, and regulations influence clinical practice. The objective was to document physicians’ clinical practice surrounding pharmacologic, nutritional, and herbal products for the management of low milk supply.Methods:Physicians from two Facebook groups completed an anonymous, online survey about their clinical use of pharmaceutical, supplemental, and nutritional substances perceived to increase milk production. The association between practice patterns and lactation medicine expertise or country for different perceived galactagogues was determined.Results:A total of 307 physicians completed the primary portion of the survey. There was no difference in the proportion of physicians in Canada or the United States (U.S.) who were defined as lactation medicine experts. Canadian physicians were more likely to prescribe metformin, levothyroxine, and domperidone for low milk supply compared with U.S.-based physicians. Canadians and experts were more likely than U.S.-based and nonexpert physicians, respectively, to include herbs in their practice. Patient request, personal and clinical experience, and lack of education, research, or access were frequently selected as reasons for or against using a specific substance.Conclusions:The U.S. Food and Drug Administration ban on domperidone affects low milk supply management in North America. Though Canadian physicians prescribed domperidone without limitations, U.S. physicians were less likely to incorporate it into clinical practice. Despite a lack of evidence, physicians in North America frequently used herbs and foods to increase milk production.

Evaluation of Lipin 1 polymorphisms for genetic markers in association with performance traits in Azikheli buffalo

Lipin 1 regulates lipid metabolism in mammary glands. Genetic variation in lipin 1 gene is thought to effect milk production in dairy animals. This study aimed to investigate the association of SNPs in the exon 6 and 8 of the lipin 1 gene with milk yield in Azikheli buffalo from Swat, Pakistan. Azikheli buffaloes raised in a farm were selected based on their milk yield records. Three buffaloes were selected from the high milk yielding group with an average production of 12.96 ± 0.83 liters per day, and three from low yielding group producing 6.12 ± 0.28 liters of milk per day. Genomic DNA was extracted from the blood samples of these buffaloes, which were used to amplify exon 6, 8 the adjacent introns (6, 7 and 8) of the lipin 1 gene. The PCR products was sequenced through sanger sequencing. A total of 45 SNPs were found in the targeted regions of the lipin 1 gene in both high and low yielding buffaloes. Overall, four missense variants (g.62048T>C; Met399Thr and g.62069C>A; Pro406His) were found in the exon 8 of lipin 1 gene in Azikheli buffalo. Milk yield was positive correlated with SNP g.62048T>C; Met399Thr that was more frequent (0.83 vs 0.33) in high milk yielding buffaloes compared to the low yielding group. SNP g.62069C>A; Pro406His was unique to low milk yielding buffaloes suggesting its negative association with milk yield. Moreover, the visualization of 3D structure of the lipin I protein constructed using Phyr2 web server and the Missense 3D analysis identified no structural damages caused by the reported SNPs on the target protein. In conclusion, SNPs g.62048T>C; Met399Thr and g.62069C>A; Pro406His could serve as potential genetic markers for increasing milk productivity in buffaloes.

Narrative Review of Known Plants which Have Potential Benefits as Natural Galactagogues in Indonesia

Human milk is the gold standard for infant nutrition during the first six months of life. According to Indonesia Demographic and Health Survey in 2017, about 45% of mothers in Indonesia provided pre-lacteal feeding within three days postpartum. Low or no milk production and perceived insufficient milk for the infants need were common reasons for pre-lacteal feeding. Galactagogues are believed to help augment human milk production. Therefore, galactagogue consumption is highly recommended, especially natural galactagogue, to help overcome low milk production problem. Most natural galactagogues are used as a local tradition only. Scientific studies regarding the phytochemical compounds and the mechanisms involved are still very limited. This narrative review aimed to describe selected research articles reporting the potency of torbangun, katuk, kelor, and papaya leaves as known plants acting as natural galactagogues in Indonesia. A total of 22 original research articles from Indonesia written in Indonesian or English over a 20 years period were included in this review. Studies were selected if selected natural galactagogues were evaluated at least for the proposed mechanism of action and effects. The data have shown strong evidence about the positive effect of torbangun, katuk, kelor, and papaya leaves as natural galactagogues on increasing human milk production. The five leaves contain different phytoestrogens that have an estrogen effect to stimulate prolactin production and increase milk production.

Edukasi Tanaman Herbal sebagai Booster ASI di Desa Rindu Hati Kecamatan Taba Penanjung, Bengkulu Tengah

One of the failures of exclusive breastfeeding is the mother’s perception that breast milk production is small or insufficient, giving rise to concerns that the baby will not be able to get enough. Another problem that arises if exclusive breastfeeding coverage is inadequate is nutritional problems and this causes stunting. Efforts made to increase mother’s knowledge about increasing breast milk boosters, where these local plants are Moringa leaves and Kepok banana blossoms. The aim of implementing this educational activity is to change the knowledge of educational participants about the use of local plants as breast milk boosters. The activity will be carried out on October 18, 2023 where the increase in knowledge can be seen from pre-test, given education using prepared materials, and continued post-test. This activity was attended by 22 breasfeeding mothers who have children in Rindu Hati Village, Taba Penanjung District, Central Bengkulu Regency. The evalution result during the activity showed that the participants were very enthusiastic and had active discussions. There was an increase in knowledge of breastfeeding mothers after aducation was conducted about the use of local plants as breast milk boosters in Rindu Hati Village. It is hoped that breastfeeding mothers can apply the knowledge gained so that breastfeeding mothers’ breast milk production increases and reduce mothers’ concerns about low breast milk production by utilizing Moringa leaves and Kepok banana blossoms which are easily available around the home.

Effects of Elevating Zinc Supplementation on the Health and Production Parameters of High-Producing Dairy Cows

This study’s objective was to determine the effects of increasing the dietary added zinc (Zn) on the milk production, milk somatic cell count (SCC), and immunoglobulin and antioxidant marker concentrations in the blood of dairy cows. Twelve Holstein cows (67 ± 2.5 days in milk) were assigned randomly to (1) a diet containing Zn–methionine at 76 mg/kg of DM (CTL) or (2) CTL top-dressed with about 21 mg/kg of DM extra Zn–methionine (+Zn) for 70 d. The concentrations of reduced (GSH) and oxidized (GSSG) glutathione, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and immunoglobulins in the blood were measured on d 0, 35, and 70. Compared to CTL, +Zn decreased the dry matter intake (DMI) throughout the trial and the milk yield (MY) during the first phase of feeding (0–35 d). It, however, increased the milk yield during the last phase (36–70 d). The +Zn tended to have lower and greater milk protein yields than CTL during the first and last feeding phases, respectively. The +Zn tended to decrease the SCC and was associated with lower plasma GSH: GSSG and lower serum SOD concentrations relative to CTL. The +Zn did not affect the immunoglobulins, MDA, or CAT. Despite the early DMI and MY reduction, the prolonged Zn–methionine supplementation at about 100 mg/kg of DM improved the milk yield, possibly as a result of the improved udder health of dairy cows.