Low Graphene Oxide Concentration Research Articles

Ascorbic Acid and Graphene Oxide Exposure in the Model Organism Acheta domesticus Can Change the Reproduction Potential.

The use of nanoparticles in the industry carries the risk of their release into the environment. Based on the presumption that the primary graphene oxide (GO) toxicity mechanism is reactive oxygen species production in the cell, the question arises as to whether well-known antioxidants can protect the cell or significantly reduce the effects of GO. This study focused on the possible remedial effect of vitamin C in Acheta domesticus intoxicated with GO for whole lives. The reproduction potential was measured at the level of Vitellogenin (Vg) gene expression, Vg protein expression, hatching success, and share of nutrition in the developing egg. There was no simple relationship between the Vg gene's expression and the Vg protein content. Despite fewer eggs laid in the vitamin C groups, hatching success was high, and egg composition did not differ significantly. The exceptions were GO20 and GO20 + Vit. C groups, with a shift in the lipid content in the egg. Most likely, ascorbic acid impacts the level of Vg gene expression but does not affect the production of Vg protein or the quality of eggs laid. Low GO concentration in food did not cause adverse effects, but the relationship between GO toxicity and its concentration should be investigated more thoroughly.

Eco-Friendly Waterborne Polyurethane Coating Modified with Ethylenediamine-Functionalized Graphene Oxide for Enhanced Anticorrosion Performance.

This study explores the potential of graphene oxide (GO) as an additive in waterborne polyurethane (WPU) resins to create eco-friendly coatings with enhanced anticorrosive properties. Traditionally, WPU's hydrophilic nature has limited its use in corrosion-resistant coatings. We investigate the impact of incorporating various GO concentrations (0.01, 0.1, and 1.3 wt%) and functionalizing GO with ethylenediamine (EDA) on the development of anticorrosive coatings for carbon steel. It was observed, by potentiodynamic polarization analysis in a 3.5% NaCl solution, that the low GO content in the WPU matrix significantly improved anticorrosion properties, with the 0.01 wt% GO-EDA formulation showing exceptional performance, high Ecorr (-117.82 mV), low icorr (3.70 × 10-9 A cm-2), and an inhibition corrosion efficiency (η) of 99.60%. Raman imaging mappings revealed that excessive GO content led to agglomeration, creating pathways for corrosive species. In UV/condensation tests, the 0.01 wt% GO-EDA coating exhibited the most promising results, with minimal corrosion products compared to pristine WPU. The large lateral dimensions of GO sheets and the cross-linking facilitated by EDA enhanced the interfacial properties and dispersion within the WPU matrix, resulting in superior barrier properties and anticorrosion performance. This advancement underscores the potential of GO-based coatings for environmentally friendly corrosion protection.

Graphene Oxide as a Factor Modifying the Properties of Wood

This work carried out research to determine the possibilities of using graphene oxide to provide wood with new functional features. With the saturation parameters used and working liquid with a concentration of 0.004% graphene oxide, the retention of the nanomaterial in wood was 0.25 kg/m3. The presence of graphene oxide increased the crystallinity of the wood to 64% (compared with 57% for unmodified wood). The TG/DTG spectra of wood impregnated with graphene oxide and the control wood indicated that the initial weight loss of the samples observed at a temperature of 100 °C was similar and amounted to less than 4%. A second mass loss was observed in a temperature range of 270 to 380 °C. The mass loss in this temperature range reached 70% and was similar in the test and control samples. Wood modified with graphene oxide showed increased thermal stability in a temperature range of 360 to 660 °C compared with native wood. Given the results obtained, there were no statistically significant differences in the water absorption of modified or control wood. The presence of low concentrations of graphene oxide in the culture medium did not inhibit the growth of the fungus Trichoderma viride; however, a decrease in the growth activity of mycelial hyphae was observed with an increasing concentration of nanomaterial in the medium. It has been reported that graphene oxide, as a stress factor, initiates changes at the cellular level, characterized by the formation of structures called chlamydospores by the body.

Corrosion resistance and tribological behavior of FeCoCrNi@GO/Ni high entropy alloy-based composite coatings prepared by electrodeposition

The preparation of high entropy alloy (HEA) coatings onto a stainless-steel substrate is crucial to pursue improved corrosion and wear resistance but are still challenging. Herein, FeCoCrNi@GO/Ni coatings are innovatively cross-integrated with stainless steel by introducing a nickel transition layer to break through the “bottleneck” of stainless steel. Meanwhile, variation of the coating corrosion resistance in 3.5 wt% NaCl solution and tribological behavior against GCr15 steel balls was also deeply explored. The addition of graphene oxide (GO) delayed the movement of corrosive medium and improved the corrosion resistance. Interestingly, the coatings with low GO content and high GO content show different wear mechanisms respectively, and the lower GO content is conducive to the formation of oxidized lubrication layer, thus protecting the substrate from severe friction and wear. However, the high content of GO coating will cause damage and spalling of oxide layer and GO, and the wear mechanism changes from oxidative wear to the co-wear of oxidative wear and abrasive wear. This work will provide a novel idea for the development of HEA coatings in the future.

Adsorption-solubilization controlled extraction of pyrene from surfactant solutions by graphene oxide

Graphene and its derivatives such as graphene oxide (GO) has attracted increasing attention in water treatment because of its unique physical and chemical properties. Herein, the adsorption performance of GO for a typical polycyclic aromatic hydrocarbon (PAH), pyrene from solutions of either a non-ionic surfactant Tergitol 15-S-7 or an anionic surfactant sodium dodecyl benzene sulphonate (SDBS) is investigated. It demonstrates that the high affinity of GO to pyrene leads to multi-layer adsorption of pyrene, with an adsorption capacity 2 to 3 times of its own weight at a low GO concentration of 3.9 mg/L. Linear two-zone pyrene adsorption isotherms are identified, which should be due to competitive adsorption-solubilization processes of pyrene. Although Tergitol 15-S-7 has a considerably higher solubilization capacity (affinity) for pyrene compared to SDBS, faster adsorption kinetics, larger solid-liquid distribution coefficients, and higher recovery of pyrene from Tergitol 15-S-7 solutions are achieved. The higher affinity of SDBS to GO leads to its competitive adsorption that impairs the adsorption of pyrene by GO. Furthermore, the GO demonstrates excellent adsorption capacity for pyrene even after 5 recycling cycles.

Prediction Model and Mechanism for Drying Shrinkage of High-Strength Lightweight Concrete with Graphene Oxide.

The excellent performance of graphene oxide (GO) in terms of mechanical properties and durability has stimulated its application potential in high-strength lightweight concrete (HSLWC). However, more attention needs to be paid to the long-term drying shrinkage of HSLWC. This work aims to investigate the compressive strength and drying shrinkage behavior of HSLWC incorporating low GO content (0.00-0.05%), focusing on the prediction and mechanism of drying shrinkage. Results indicate the following: (1) GO can acceptably reduce slump and significantly increase specific strength by 18.6%. (2) Drying shrinkage increased by 8.6% with the addition of GO. A modified ACI209 model with a GO content factor was demonstrated to have high accuracy based on the comparison of typical prediction models. (3) GO not only refines the pores but also forms flower-like crystals, which results in the increased drying shrinkage of HSLWC. These findings provide support for the prevention of cracking in HSLWC.

The combination of graphene oxide and preservatives can further improve the preservation of cut flowers.

It is reported that the use of nanomaterials can extend the vase life of fresh-cut flowers. Graphene oxide (GO) is one of these nanomaterials that aid in promoting water absorption and antioxidation during the preservation of fresh-cut flowers. In this investigation, the three mainstream brands of preservatives commercially available on the market ("Chrysal," "Floralife," and "Long Life") in combination with low concentrations of GO (0.15 mg/L) were used to preserve fresh-cut roses. The results showed that the three brands of preservatives had different degrees of freshness retention. Compared to the preservatives used alone, the combination of low concentrations of GO with the preservatives, especially in the L+GO group (with 0.15 mg/L GO added in the preservative solution of "Long life"), further improved the preservation of cut flowers. L+GO group showed less level of antioxidant enzyme activities, lower ROS accumulation and cell death rate, and higher relative fresh weight than the other groups, implying a better antioxidant and water balance abilities. GO attached to the xylem duct of flower stem, and reduced the blockage of xylem vessels by bacteria, which were determined by SEM (scanning electron microscopy) and FTIR (Fourier transform infrared) analysis. XPS (X-ray photoenergy spectra) analysis results proved that GO could enter the interior of flower stem through xylem duct, and when combined with "Long Life," the anti-oxidation protection ability of GO was enhanced, thus delaying ageing, and greatly extending the vase life of fresh-cut flowers. The study provides new insights into cut flower preservation using GO.

GO-Enabled Bacterial Cellulose Membranes by Multistep, In Situ Loading: Effect of Bacterial Strain and Loading Pattern on Nanocomposite Properties.

This paper presents the results of research on the preparation and properties of GO/BC nanocomposite from bacterial cellulose (BC) modified with graphene oxide (GO) using the in situ method. Two bacterial strains were used for the biosynthesis of the BC: Komagataeibacter intermedius LMG 18909 and Komagataeibacter sucrofermentans LMG 18788. A simple biosynthesis method was developed, where GO water dispersion was added to reinforced acetic acid-ethanol (RAE) medium at concentrations of 10 ppm, 25 ppm, and 50 ppm at 24 h and 48 h intervals. As a result, a GO/BC nanocomposite membrane was obtained, characterized by tensile strength greater by 150% as compared with the pure BC (̴ 50 MPa) and lower volume resistivity of ~4 ∙ 109 Ω × cm. Moreover, GO addition increases membrane thickness up to ~10% and affects higher mass production, especially with low GO concentration. All of this may indicate the possibility of using GO/BC membranes in fuel cell applications.

Large-scale synthesis of 2D-silica (SiOx) nanosheets using graphene oxide (GO) as a template material.

Graphene oxide (GO) was used in this study as a template to successfully synthesize silicon oxide (SiOx) based 2D-nanomaterials, adapting the same morphological features as the GO sheets. By performing a controlled condensation reaction using low concentrations of GO (<0.5 wt%), the study shows how to obtain 2D-nanoflakes, consisting of GO-flakes coated with a silica precursor that were ca. 500 nm in lateral diameter and ca. 1.5 nm in thickness. XPS revealed that the silanes had linked covalently with the GO sheets at the expense of the oxygen groups present on the GO surface. The GO template was shown to be fully removable through thermal treatment without affecting the nanoflake morphology of the pure SiOx-material, providing a methodology for large-scale preparation of SiOx-based 2D nanosheets with nearly identical dimensions as the GO template. The formation of SiOx sheets using a GO template was investigated for two different silane precursors, (3-aminopropyl) triethoxysilane (APTES) and tetraethyl orthosilicate (TEOS), showing that both precursors were capable of accurately templating the graphene oxide template. Molecular modeling revealed that the choice of silane affected the number of layers coated on the GO sheets. Furthermore, rheological measurements showed that the relative viscosity was significantly affected by the specific surface area of the synthesized particles. The protocol used showed the ability to synthesize these types of nanoparticles using a common aqueous alcohol solvent, and yield larger amounts (∼1 g) of SiOx-sheets than what has been previously reported.

Carbon sequestration in graphene oxide modified cementitious system

Graphene oxide is emerging as a promising nanomaterial owing to its unique property of being a strong and flexible material. This work examines the potential use of graphene oxide (GO) for CO2 sequestration in cementitious-based materials in addition to mechanical properties. The effect of oxidized graphene surface on the hydration mechanism of cement paste and carbonate mineralization was investigated. Experimental results show that the application of GO at optimum amount offers 40% improvement in compressive strength at an early age. Accelerated carbon curing of GO-cement matrix was performed to evaluate CO2 sequestration through thermogravimetric analysis and results revealed that adding a low concentration of GO (i.e. 0.05%) improved CO2 uptake by 30% in the cementitious system. Further, physical adsorption of CO2 molecules and alteration in C–S–H was confirmed by the FTIR study. This finding suggests that the application of GO, not only improved the mechanical properties but it significantly enhanced the CO2 sequestration in cementitious systems.

Structure and properties of starch/chitosan food packaging film containing ultra-low dosage GO with barrier and antibacterial

Using urea as an intermediate, graphene oxide (GO) was modified with carboxymethyl cellulose (CMC) to obtain a uniformly dispersed GO monolayer dispersion for starch/chitosan composite preparation films. The structure and properties of modified GO and starch/chitosan composite films were characterized by FTIR, XRD, and TG. It was found that the modified GO could form a single layer uniformly and stably dispersed in the starch/chitosan composite film. At low GO content (0.01 wt%), the tensile properties of the films increased by 91.7% to 62.5 MPa compared with the control group, the water vapor permeability (WVP) was low, the water resistance was good, and the UV shielding effect was maintained at 92% under 50% visible light. At the same time, the film changed from overflow antibacterial mode to contact antibacterial mode. The antibacterial ability against Escherichia coli and Staphylococcus aureus increased by 23.8% and 25.6%, respectively, compared with the control group. It has an apparent preservation ability for fresh fruit. These excellent characteristics can be widely used in food packaging and pharmaceutical fields.

Effect of graphene oxide on the uptake, translocation and toxicity of metal mixture to Lepidium sativum L. plants: Mitigation of metal phytotoxicity due to nanosorption

Due to its unique structure and exceptional properties, graphene oxide (GO) is increasingly used in various fields of industry and therefore is inevitably released into the environment, where it interacts with different contaminants. However, the information relating to the ability of GO to affect the toxicity of contaminants is still limited. Therefore, the aim of our study was to synthesize GO, to examine the phytotoxicity of different concentrations of GO and its co-exposure with the metal mixture using garden cress (Lepidium sativum L.) as a test organism and to evaluate the potential of GO to affect toxicity of metals and their uptake by plants. The metal mixture (MIX) containing Ni (II), Zn (II), Cr (III) and Cu (II) was prepared in accordance with the maximum-permissible-concentrations (MPC) accepted for the inland waters in the EU. Additionally, the capacity of GO to adsorb metals was studied in specific conditions of the phytotoxicity test and assessed using adsorption isotherms. Our data indicate that in most cases the tested concentrations of MIX, GO and MIX + GO did not affect seed germination, root growth and biomass of roots and seedlings, however, they were found to alter photosynthesis processes, enhance production of carotenoids and H2O2 as well as to activate lipid peroxidation. Additionally, our study revealed that GO affects the accumulation of tested metals in roots and shoots of the MIX-exposed L. sativum. This is due to the capacity of GO to adsorb metals from the growth medium. Therefore, low concentrations of GO can be used for water decontamination.

Graphene oxide decreases the abundance of nitrogen cycling microbes and slows nitrogen transformation in soils

Graphene oxide (GO) has been widely used in many applications due to its excellent properties. Given the extensive production and use of this nanomaterial, its release into the environment is inevitable. However, little is known about the effects of GO on microbial nitrogen transformation and the related processes after GO enters the soil environment. The present study showed that GO significantly reduced soil microbial biomass and caused a decline in microbial diversity after the soils were subjected to various GO concentrations (10, 100, and 1000 mg kg−1) for 4 months. Among them, the abundances of nitrogen transformation related bacteria such as Firmicutes, Nitrospirota, Proteobacteria, Planctomycetota, and Cyanobacteria were significantly decreased with GO incubation. Among the enzymes that are related to nitrogen transformation, nitrate reductase was the most sensitive even at low concentrations of GO, followed by ammonia monooxygenase and urease, which were reduced by 13–31%, 5–26%, and 9–19% respectively, than those of the control. We found that high concentrations of GO significantly increased the retention of soil urea by 32–59%, and the contents of ammonium and nitrate were 22–28% and 55–69% lower compared to those of the control, respectively. Moreover, the response of most of the indicators in the above process to multilayer GO was more significant than that to single layer GO. Overall, this study provides new insights into the comprehensive understanding of GO's impacts on the soil nitrogen cycle.

Preparation of functional copolymer based composite membranes containing graphene oxide showing improved electrochemical properties and fuel cell performance

The objective of this work is to prepare a functional copolymer of poly(acrylonitrile)-co-poly(2-Acrylamido-2-methyl-1-propanesulfonic acid) (PAN-co-PAMPS) and impregnation of graphene oxide (GO) into the copolymer followed by crosslinking to prepare conetwork composite membranes by simple and cost effective solution casting method and evaluating their structural, morphological, thermal, and mechanical properties. The successful incorporation of different amounts of GO content (0.1–1 wt%) within the polymer matrix was confirmed by FT-IR spectroscopy, X-ray diffraction, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The mechanical properties of the prepared crosslinked composite membranes are found to be greatly enhanced by the addition of GO in the copolymer matrix. The thermogravimetric analysis (TGA) demonstrated considerable improvements in thermal stability for the composite membrane with low GO content. The effect of loading of GO in the copolymer matrix on proton conductivity and fuel cell performance has been studied systematically. The membranes prepared by mixing with 0.5 wt% GO in the copolymer followed by crosslinking exhibited maximum ionic conductivity (Km), lower methanol permeability (PM), and higher relative selectivity. This observed PM value is much lower range from 3.02 × 10−7 to 11.9 × 10−7 cm2/s compared to the Nafion® 117 membrane (22 × 10−7 cm2/s). The fuel cell performance in terms of maximum power density and current density and the durability of the crosslinked composite membranes have also been evaluated here. Low PM, high Km, and high selectivity values show that functional co-polymer/GO crosslinked co-network composite membrane is a promising alternative membrane separator to replace the expensive Nafion® 117 for proton exchange membrane fuel cells (PEMFCs) application.

Enhanced biohydrogen production with low graphene oxide content using thermophilic bioreactors

Modern society envisions hydrogen (H2) fuel to drive the transportation, industrial, and domestic sectors. Here, we explore use of graphene oxide nanoparticles (GO NPs) for greatly enhancing bio-H2 production by a consortium based on Thermoanaerobacterium thermosaccharolyticum spp. Thermophilic batch bioreactors were set up at 60 OC and initial pH of 8.5 to assess the effects of GO NPs supplements on biohydrogen production. Under optimal GO NPs loading of 10 mg/L, the supplemented system yielded ∼ 300% higher H2 yield (6.78 mol H2/mol sucrose) than control. Such an optimized system offered 73% H2 purity and 85% conversion efficiency by promoted the desirable acetate fermentation pathway. Miseq Illumina sequencing tests revealed that the optimal levels of GO NPs did not alter the microbial composition of consortium.

In situ control of graphene oxide dispersions with a small impedance sensor

Carbon-based nanomaterials (CBNs), such as graphene and carbon nanotubes, display advanced physical and chemical properties, which has led to their widespread applications. One of these applications includes the incorporation of CBNs into cementitious materials in the form of aqueous dispersions. The main issue that arises in this context is that currently no established protocol exists as far as characterizing the dispersions. In the present article, an innovative method for quick evaluation and quantification of graphene oxide (GO) dispersions is proposed. The proposed method is electrical impedance spectroscopy (EIS) with an impedance sensor. The novelty lies on the exploitation of a small sensor for on-site (field) direct dielectric measurements with the application of alternating current. Five different concentrations of GO dispersions were studied by applying EIS and for various accumulated ultrasonic energies. The low GO concentration leads to high impedance values due to low formed current network. Two opposing mechanisms were revealed during the accumulation of ultrasonic energy, that are taking place simultaneously: breakage of the agglomerates that facilitates the flow of the electric current due to the formation of a better dispersed network, nevertheless the surface hydrophilic structure of the GO is damaged with the high accumulated ultrasonic energy. The dielectric measurements were exploited to express an appropriate quantitative ‘quality index’ to facilitate with the dispersion control of the nanostructures. An intermediate concentration of GO is suggested (about 0.15 wt% of the binder materials) to be optimal for the specific engineering application, ultrasonicated at approximately 30 to 65 kJ. The investigated methodology is highly novel and displays a high potential to be applied in-field applications where CBNs must be incorporated in building materials.

Effects of Three Carbonaceous Nanomaterials on the Developmental Toxicity, Oxidative Stress, and Metabolic Profile in Zebrafish

As an important part of artificial nanomaterials, carbonaceous nanomaterials (CNMs) are widely applied in a plenty of areas such as energy, manufacturing and pharmaceutical industries. In the present study, the developmental toxicity, induced by three typical CNMs including graphene oxide (GO), carbon nanotube (CNT) and graphene oxide quantum dot (GOQD) was investigated in the typical model animal, zebrafish larva. The induced sub-acute toxicity at the low concentration of GO, CNT and GOQD was investigated in adult zebrafish, either. Moreover, the molecular mechanisms at the level of metabolomics were also explored. The results showed that there was a significant increase in reactive oxygen species (ROS), and mitochondrial membrane damage was caused by GO, CNT and GOQD in zebrafish larva. However, there was no significant developmental toxicity on zebrafish larva. The toxicity order in terms of the ROS increase and mitochondrial membrane damage was GOQD > CNT > GO. The chronic exposure at the typical environment-associated concentration (0.01 mg·L-1) of CNMs can induce gill and kidney cell senescence of adult zebrafish. Meanwhile, it can also inhibit total superoxide dismutase (T-SOD) activity in adult zebrafish in the subacute toxicity test (21 d) at the concentration of 0.01 mg·L-1. The metabolomics research revealed that the toxicity order at the environment-associated concentration acting on adult zebrafish was GOQD > CNT > GO; and it showed that fatty acids and proline turbulence may be responsible for one of the molecular mechanisms of T-SOD inhibition in adult zebrafish. This work can supply rationale to evaluate the potential risk of ecosystems and human health induced by the three typical CNMs.

Graphene Oxide-Induced Protein Conformational Change in Nasopharyngeal Carcinoma Cells: A Joint Research on Cytotoxicity and Photon Therapy.

The objectives of this work aim to investigate the interaction and cytotoxicity between nanometric graphene oxide (GO) and nasopharyngeal carcinoma cells (NPC-BM1), and possible application in photon therapy. GO nanosheets were obtained in the size range of 100–200 nm, with a negative surface charge. This nanometric GO exhibited a limited (<10%) cytotoxicity effect and no significant dimensional change on NPC-BM1 cells in the tested GO concentration range (0.1–10 µg·mL−1). However, the secondary protein structure was modified in the GO-treated NPC-BM1 cells, as determined through synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIRM) mapping. To further study the cellular response of GO-treated NPC-BM1 cancer cells at low GO concentration (0.1 µg·mL−1), photon radiation was applied with increasing doses, ranging from 2 to 8 Gy. The low radiation energy (<5 Gy) did not cause significant cell mortality (5–7%). Increasing the radiation energy to 6–8 Gy accelerated cell apoptosis rate, especially in the GO-treated NPC-BM1 cells (27%). This necrosis may be due to GO-induced conformational changes in protein and DNA/RNA, resulting in cell vulnerability under photon radiation. The findings of the present work demonstrate the potential biological applicability of nanometric GO in different areas, such as targeted drug delivery, cellular imaging, and radiotherapy, etc.

Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells

Background: The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodeg-radability. Thus, the two-dimensional graphene oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture. In this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. Results: It was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. Conclusions: The adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.

Self-assembling graphene oxide/modified amphipathic hydroxyethyl cellulose hybrid stabilized Pickering emulsion polymerization for functional hydrogel

Herein, a self-assembled hybrid from bromoacetic acid (BA) modified amphipathic hydroxyethyl cellulose (MAHEC) and graphene oxide (GO) was prepared and used as Pickering emulsifiers to synthesizing functional porous hydrogel by removing the oil phase after emulsion templating polymerization. The MAHEC grafted with the 1-bromododecane was prepared by the phase-transfer method, and the graphene oxide was prepared by Hummers’ method. The interaction between the GO nanosheets and MAHEC molecular chain was investigated by X-Ray Diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The amphipathy of the GO and MAHEC was measured by the water contact angle, which was 74.0° and 103.0°, respectively. A stable, low cost, and biocompatible Pickering emulsion was fabricated by a low concentration of GO and MAHEC hybrid. Stabilization of Pickering emulsion prepared by the nanohybrid emulsifier GO and MAHEC at different conditions was investigated, such as different electrolyte concentration, pH, and oil/water ratio. The polyacrylamide (PAM) organo-hydrogel was polymerized by the stable Pickering emulsion template. After removing the olive oil, the inner wall of the porous hydrogel was coated by the MAHEC and GO for the pH-sensitive release. The strategy of Pickering emulsion templating polymerization by utilizing functional emulsifiers could be applied for the preparation of various smart materials.