Low Acoustic Intensity Research Articles

Enhancing Image Contrast in Breast USCT Reflection Imaging Using Coherence Factor Beamforming

Abstract Breast cancer is the most common cancer worldwide, and early screening is essential. Existing breast ultrasound is affordable and convenient, but its 2D image quality is poor, and it heavily relies on the doctor’s skills. The novel breast ultrasound computed tomography (USCT) can use a ring array probe to implement hundreds of single transducer emission for full-view focusing synthetic aperture reflection imaging, thereby obtaining high-resolution 3D images. However, single transducer emission results in low acoustic intensity, which further leads to low signal-to-noise ratio and low contrast of reflection images. To improve image contrast, this study incorporated beamforming with coherence factor (CF) into reflection imaging using ring transducers, promising to produce high-contrast USCT reflection images. For evaluation, we developed a USCT prototype with 2048 elements and conducted phantom experiments. We conducted phantom experiments. Results showed that the contrast achieved by the coherence factor method of the breast phantom image is improved from 24.54 dB to 63.18 dB, and the artifacts are significantly suppressed. It can be seen that the CF method could significantly improve the contrast of the breast USCT reflection image.

Effects of different sonication parameters of theta burst transcranial ultrasound stimulation on human motor cortex

BackgroundTheta burst TUS (tbTUS) can induce increased cortical excitability in human, but how different sonication parameters influence the effects are still unknown. ObjectiveTo examine how a range of sonication parameters, including acoustic intensity, pulse repetition frequency, duty cycle and sonication duration, influence the effects of tbTUS on human motor cortical excitability. Methods14 right-handed healthy subjects underwent 8 sessions with different tbTUS parameters in a randomized, cross-over design on separate days. The original tbTUS protocol was studied in one session and one parameter was changed in each of the seven sessions. To examine changes in cortical excitability induced by tbTUS, we measured the motor-evoked potential (MEP) amplitude, resting motor threshold, short-interval intracortical inhibition and intracortical facilitation, as well as short-interval intracortical facilitation before and up to 90 min after tbTUS. ResultsAll conditions increased MEP amplitudes except the condition with low acoustic intensity of 10 W/cm2. Pulse repetition frequency of 5 Hz produced higher MEP amplitudes compared to pulse repetition frequencies of 2 and 10 Hz. In addition, higher duty cycles (5%, 10%, and 15%) and longer sonication durations (40, 80, and 120 s) were associated with longer duration of increased MEP amplitudes. Resting motor threshold remained stable in all conditions. For paired-pulse TMS measures, tbTUS reduced short-interval intracortical inhibition and enhanced short-interval intracortical facilitation, but had no effect on intracortical facilitation. ConclusionsUltrasound bursts repeated at theta (∼5 Hz) frequency is optimal to produce increased cortical excitability with the range of 2–10 Hz. Furthermore, there was a dose-response effect regarding duty cycle and sonication duration in tbTUS for plasticity induction. The aftereffects of tbTUS were associated with a shift of the inhibition/excitation balance toward less inhibition and more excitation in the motor cortex. These findings can be used to determine the optimal tbTUS parameters in neuroscience research and treatment of neurological and psychiatric disorders.

Theoretical research on the application of impedance calculation in the design of high-intensity low-frequency acoustic generator

The impedance matching is essential in producing high-intensity acoustic waves. This paper studies the impedance calculation methods of modulators and double-chamber Helmholtz resonators based on fluid mechanics, aeroacoustics, and electro-acoustic analogy principles. The results: ① The impedance expression of the resonators is derived. ② The impedance variation law of the chamber volume, the stator size, the air supply pressure, the air supply flow, and the working frequency is calculated. ③ The impedance-matching design scheme of the two-chamber Helmholtz low frequency and high acoustic intensity generator is given. Based on the experimental results of the high acoustic intensity generating device that the author has manufactured in the closed space, the correctness of the impedance calculation and matching design scheme in this paper is verified. This method has guiding significance for the design of high-intensity acoustic generating devices in confined space.

Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles.

High-intensity focused ultrasound (HIFU) has been widely used in tumor ablation in clinical settings. Meanwhile, there is great potential to increase the therapeutic efficiency of temporary cavitation due to enhanced thermal effects and combined mechanical effects from nonlinear vibration and collapse of the microbubbles. In this study, dual-frequency (1.1 and 5 MHz) HIFU was used to produce acoustic droplet vaporization (ADV) microbubbles from activatable perfluoropentane-loaded polymer nanoparticles (PFP@Polymer NPs), which increased the therapeutic outcome of the HIFU and helped realize tumor theranostics with ultrasound contrast imaging. Combined with PFP@Polymer NPs, dual-frequency HIFU changed the shape of the damage lesion and reduced the acoustic intensity threshold of thermal damage significantly, from 216.86 to 62.38 W/cm2. It produced a nearly 20 °C temperature increase in half the irradiation time and exhibited a higher tumor inhibition rate (84.5% ± 3.4%) at a low acoustic intensity (1.1 MHz: 23.77 W/cm2; 5 MHz: 0.35 W/cm2) in vitro than the single-frequency HIFU (60.2% ± 11.9%). Moreover, compared with the traditional PFP@BSA NDs, PFP@Polymer NPs showed higher anti-tumor efficacy (81.13% vs. 69.34%; * p < 0.05) and better contrast-enhanced ultrasound (CEUS) imaging ability (gray value of 57.53 vs. 30.67; **** p < 0.0001), probably benefitting from its uniform and stable structure. It showed potential as a highly efficient tumor theranostics approach based on dual-frequency HIFU and activatable PFP@Polymer NPs.

Non-planar holographic lens for mild hyperthermia in a broad area

High intensity focused ultrasound (HIFU) has been used as a non-invasive tool for thermal ablation treatments. Typically, HIFU can ablate a small region (2 × 10 mm2) depending on the frequency and transducer dimensions. Cavitation-induced bubbles or injected microbubbles have been shown to enhance tissue heating during HIFU at lower acoustic intensities. The formed lesion is limited to the focal region, necessitating prolonged time of treatment to expose the entire target. To solve this problem, we designed a non-planar diffraction lens that mounts onto the surface of a 900kHz focused transducer (64 mm diameter and 62 mm focus) to enlarge the focal region. The lens was designed with an iterative angular spectrum approach to achieve a lateral beamwidth of 8 mm while maintaining a similar axial beamwidth. The focal beam size (transverse × axial) increased from 2.5 × 15.8 mm2 to 8 × 20 mm2 which also resulted in a corresponding increase in thermal lesion. We plan to expand this work to matrix phased arrays for application to mild hyperthermia for enhanced drug delivery.

Visual prediction cues can facilitate behavioural and neural speech processing in young and older adults

The ability to process speech evolves over the course of the lifespan. Understanding speech at low acoustic intensity and in the presence of background noise becomes harder, and the ability for older adults to benefit from audiovisual speech also appears to decline. These difficulties can have important consequences on quality of life. Yet, a consensus on the cause of these difficulties is still lacking. The objective of this study was to examine the processing of speech in young and older adults under different modalities (i.e. auditory [A], visual [V], audiovisual [AV]) and in the presence of different visual prediction cues (i.e., no predictive cue (control), temporal predictive cue, phonetic predictive cue, and combined temporal and phonetic predictive cues). We focused on recognition accuracy and four auditory evoked potential (AEP) components: P1–N1–P2 and N2. Thirty-four right-handed French-speaking adults were recruited, including 17 younger adults (28 ± 2 years; 20–42 years) and 17 older adults (67 ± 3.77 years; 60–73 years). Participants completed a forced-choice speech identification task. The main findings of the study are: (1) The faciliatory effect of visual information was reduced, but present, in older compared to younger adults, (2) visual predictive cues facilitated speech recognition in younger and older adults alike, (3) age differences in AEPs were localized to later components (P2 and N2), suggesting that aging predominantly affects higher-order cortical processes related to speech processing rather than lower-level auditory processes. (4) Specifically, AV facilitation on P2 amplitude was lower in older adults, there was a reduced effect of the temporal predictive cue on N2 amplitude for older compared to younger adults, and P2 and N2 latencies were longer for older adults. Finally (5) behavioural performance was associated with P2 amplitude in older adults. Our results indicate that aging affects speech processing at multiple levels, including audiovisual integration (P2) and auditory attentional processes (N2). These findings have important implications for understanding barriers to communication in older ages, as well as for the development of compensation strategies for those with speech processing difficulties.

Neuromodulation Effect of Very Low Intensity Transcranial Ultrasound Stimulation on Multiple Nuclei in Rat Brain.

ObjectiveLow-intensity transcranial ultrasound stimulation (TUS) is a non-invasive neuromodulation technique with high spatial resolution and feasible penetration depth. To date, the mechanisms of TUS modulated neural oscillations are not fully understood. This study designed a very low acoustic intensity (AI) TUS system that produces considerably reduced AI Ultrasound pulses (ISPTA < 0.5 W/cm2) when compared to previous methods used to measure regional neural oscillation patterns under different TUS parameters.MethodsWe recorded the local field potential (LFP) of five brain nuclei under TUS with three groups of simulating parameters. Spectrum estimation, time-frequency analysis (TFA), and relative power analysis methods have been applied to investigate neural oscillation patterns under different stimulation parameters.ResultsUnder PRF, 500 Hz and 1 kHz TUS, high-amplitude LFP activity with the auto-rhythmic pattern appeared in selected nuclei when ISPTA exceeded 12 mW/cm2. With TFA, high-frequency energy (slow gamma and high gamma) was significantly increased during the auto-rhythmic patterns. We observed an initial plateau in nuclei response when ISPTA reached 16.4 mW/cm2 for RPF 500 Hz and 20.8 mW/cm2 for RPF 1 kHz. The number of responding nuclei started decreasing while ISPTA continued increasing. Under 1.5 kHz TUS, no auto-rhythmic patterns have been observed, but slow frequency power was increased during TUS. TUS inhibited most of the frequency band and generated obvious slow waves (theta and delta band) when stimulated at RPF = 1.5 kHz, ISPTA = 8.8 mW/cm2.ConclusionThese results demonstrate that very low intensity Transcranial Ultrasound Stimulation (VLTUS) exerts significant neuromodulator effects under specific parameters in rat models and may be a valid tool to study neuronal physiology.

Safety Review and Perspectives of Transcranial Focused Ultrasound Brain Stimulation.

Ultrasound is an important theragnostic modality in modern medicine. Technical advancement of both acoustic focusing and transcranial delivery have enabled administration of ultrasound waves to localized brain areas with few millimeters of spatial specificity and penetration depth sufficient to reach the thalamus. Transcranial focused ultrasound (tFUS) given at a low acoustic intensity has been shown to increase or suppress the excitability of region-specific brain areas. The neuromodulatory effects can outlast the sonication, suggesting the possibility of inducing neural plasticity needed for neurorehabilitation. Increasing numbers of studies have shown the efficacy and excellent safety profile of the technique, yet comparisons among the safety-related parameters have not been compiled. This review aims to provide safety information and perspectives of tFUS brain stimulation. First, the acoustic parameters most relevant to thermal/mechanical tissue damage are discussed along with regulated parameters for existing ultrasound therapies/diagnostic imaging. Subsequently, the parameters used in studies of large animals, non-human primates, and humans are surveyed and summarized in terms of the acoustic intensity and the mechanical index. The pulse-mode operation and the use of low ultrasound frequency for tFUS-mediated brain stimulation warrant the establishment of new safety guidelines/recommendations for the use of the technique among healthy volunteers, with additional cautionary requirements for its clinical translation.

Gas-Stabilizing Sub-100 nm Mesoporous Silica Nanoparticles for Ultrasound Theranostics.

Recent studies have demonstrated that gas-stabilizing particles can generate cavitating micron-sized bubbles when exposed to ultrasound, offering excellent application potential, including ultrasound imaging, drug delivery, and tumor ablation. However, the majority of the reported gas-stabilizing particles are relatively large (>200 nm), and smaller particles require high acoustic pressures to promote cavitation. Here, this paper reports the preparation of sub-100 nm gas-stabilizing nanoparticles (GSNs) that can initiate cavitation at low acoustic intensities, which can be delivered using a conventional medical ultrasound imaging system. The highly echogenic GSNs (F127-hMSN) were prepared by carefully engineering the surfaces of ∼50 nm mesoporous silica nanoparticles. It was demonstrated that the F127-hMSNs could be continuously imaged with ultrasound in buffer or biological solutions or agarose phantoms for up to 20 min. Also, the F127-hMSN can be stored in phosphate-buffered saline for at least a month with no loss in ultrasound responsiveness. The particles significantly degraded when diluted in simulated body fluids, indicating possible biodegradation of the F127-hMSNs in vivo. Furthermore, at ultrasound imaging conditions, F127-hMSNs did not cause detectable cell death, supporting the potential safety of these particles. Finally, strong cavitation activity generation by the F127-hMSNs under high-intensity focused ultrasound insonation was demonstrated and applied to effectively ablate cancer cells.

Local anesthesia enhanced with increasing high-frequency ultrasound intensity.

The effect of local anesthetics, particularly those which are hydrophilic, such as tetrodotoxin, is impeded by tissue barriers that restrict access to individual nerve cells. Methods of enhancing penetration of tetrodotoxin into nerve include co-administration with chemical permeation enhancers, nanoencapsulation, and insonation with very low acoustic intensity ultrasound and microbubbles. In this study, we examined the effect of acoustic intensity on nerve block by tetrodotoxin and compared it to the effect on nerve block by bupivacaine, a more hydrophobic local anesthetic. Anesthetics were applied in peripheral nerve blockade in adult Sprague-Dawley rats. Insonation with 1-MHz ultrasound at acoustic intensity greater than 0.5W/cm2 improved nerve block effectiveness, increased nerve block reliability, and prolonged both sensory and motor nerve blockade mediated by the hydrophilic ultra-potent local anesthetic, tetrodotoxin. These effects were not enhanced by microbubbles. There was minimal or no tissue injury from ultrasound treatment. Insonation did not enhance nerve block from bupivacaine. Using an in vivo model system of local anesthetic delivery, we studied the effect of acoustic intensity on insonation-mediated drug delivery of local anesthetics to the peripheral nerve. We found that insonation alone (at intensities greater than 0.5W/cm2) enhanced nerve blockade mediated by the hydrophilic ultra-potent local anesthetic, tetrodotoxin. Graphical abstract.

High-intensity focused ultrasound beam path visualization using ultrasound imaging

In High-Intensity Focused Ultrasound (HIFU) treatment, effective localization of HIFU focus is important for developing a safe treatment plan. While Magnetic Resonance Imaging guided HIFU (MRIgHIFU) can visualize the ultrasound path during the treatment for localizing HIFU focus, it is challenging in ultrasound imaging guided HIFU (USIgHIFU). In the present study, a real-time ultrasound beam visualization technique capable of localizing HIFU focus is presented for USIgHIFU. In the proposed method, a short pulse, with the same center frequency of an imaging ultrasound transducer below the regulated acoustic intensity (i.e., Ispta < 720 mW/cm(2)), was transmitted through a HIFU transducer whereupon backscattered signals were received by the imaging transducer. To visualize the HIFU beam path, the backscattered signals underwent dynamic receive focusing and subsequent echo processing. From in vitro experiments with bovine serum albumin gel phantoms, the HIFU beam path was clearly depicted with low acoustic intensity (i.e., Ispta of 94.8 mW/cm(2)) and the HIFU focus was successfully localized before any damages were produced. This result indicates that the proposed ultrasound beam path visualization method can be used for localizing the HIFU focus in real time while minimizing unwanted tissue damage in USIgHIFU treatment.

Imaging Performance for Two Row-Column Arrays.

This study evaluates the volumetric imaging performance of two prototyped 62 + 62 row-column-addressed (RCA) 2-D array transducer probes using three synthetic aperture imaging (SAI) emission sequences and two different beamformers. The probes are fabricated using capacitive micromachined ultrasonic transducer (CMUT) and piezoelectric transducer (PZT) technology. Both have integrated apodization to reduce ghost echoes and are designed with similar acoustical features, i.e., 3-MHz center frequency, λ /2 pitch, and [Formula: see text] active footprint. Raw RF data are obtained using an experimental research ultrasound scanner, SARUS. The SAI sequences are designed for imaging down to 14 cm at a volume rate of 88 Hz. Two beamforming methods, spatial matched filtering and row-column adapted delay-and-sum, are used for beamforming the RF data. The imaging quality is investigated through simulations and phantom measurements. Both probes on average have similar lateral full-width at half-maximum (FWHM) values, but the PZT probe has 20% smaller cystic resolution values and 70% larger contrast-to-noise ratio (CNR) compared to the capacitive micromachined ultrasonic transducer (CMUT) probe. The CMUT probe can penetrate down to 15 cm, and the PZT probe down to 30 cm. The CMUT probe has 17% smaller axial FWHM values. The matched filter focusing shows an improved B-mode image for measurements on a cyst phantom with an improved speckle pattern and better visualization of deeper lying cysts. The results of this study demonstrate the potentials of RCA 2-D arrays against fully addressed 2-D arrays, which are low channel count (e.g., 124 instead of 3844), low acoustic intensity mechanical index (MI ≤ 0.88 and spatial-peak-temporal-average intensity [Formula: see text]), and high penetration depth (down to 30 cm), which makes 3-D imaging at high volume rates possible with equipment in the price range of conventional 2-D imaging.

Effect of seed nuclei combined with acoustic field on fine particles removal

An effective technique for fine particle removal using the combined effect of acoustic field and seed nuclei was presented. The key operation parameters, such as sound pressure level, initial particle number concentration, seed nuclei and particle properties on the agglomeration and capture performance of fine particles were investigated experimentally. The results showed that the separation of fine particle was improved efficiently with the combined external fields. Fine particle removal efficiencies increased by 25% with the combined effect of acoustic field and droplets at a SPL of 153 dB. Removal efficiency increased with the seed nuclei size. The value increased from 20% to 50% as the seed particle size increased from 10 to 30 μm, but removal efficiency showed few changes when seed particle size was larger than 30 μm. Particle removal efficiency correlated well with the initial particle number concentration in the combined effect fields. The removal efficiency with the combined effect of acoustic field and droplets was much higher than those with the combined effect of acoustic field and solid seed particles in high particle loading. Fine particle removal was significantly impacted by the particle wettability, when the combined effect of acoustic field and droplets was used. The removal efficiency of MWI particles was improved by 20% with the combined effect of acoustic field and droplets, comparing to the removal efficiency of BC particles. Fine particle removal efficiency can be highly improved with the combined external fields, especially in low acoustic intensity.

Liquid depth effect on the acoustic generation of hydroxyl radical for large scale sonochemical reactors

Abstract Liquid depth (z) is one of the most critical factors that influence the sonochemical activity, particularly for large-scale sonoreactors. This factor is one of the missing links between lab-scale sonoreactors and industrial-scale sonoreactors. Herein, computer simulations of depth effect (z = 0–8 m) on the acoustic generation of free radicals were conducted at various parameters of frequency (355–1000 kHz), acoustic intensity at the source (In,0 = 1–5 W/cm2) and bulk liquid temperature (10–40 °C). The computations were made with and without taking into account the attenuation of the ultrasound wave to concretize the influence of this event on the overall impact of depth toward the chemical activity of imploding cavities. The production of free radicals at the collapse was found to be diminished with increasing depth up to 8 m. The ultrasound wave attenuation contributed substantially in the overall reductive event, particularly at higher z and frequency and lower intensity and liquid temperature. The overall reductive effect of depth was strongly operating conditions-dependent. It was more pronounced at higher frequency (1000 kHz) and lower acoustic intensity (1 W/cm2) and bulk liquid temperature (10 °C). Depending on cavitation conditions, the attenuation of the sound wave may suppress completely the production of free radicals at small depths. All these findings were interpreted and discussed through comparison with results of previous experimental observations. In conclusion, the present computation study may furnish important data for understanding cavitation process in big-scale sonoreactors, in which attenuation of the sound wave with depth could inhibit significantly the intensity of the sonochemical process.

Removal of fine particles in WFGD system using the simultaneous acoustic agglomeration and supersaturated vapor condensation

The characteristics of fine particle removal from wet flue gas desulfurization (WFGD) using the simultaneous acoustic agglomeration and supersaturated vapor condensation were investigated experimentally. The impacts of supersaturation degree, residence time and acoustic intensity on the fine particle removal efficiency were demonstrated. High moisture and tiny droplets were contained in the flue gas after WFGD, which benefited the use of coupling external fields in WFGD system. The results showed that the removal of fine particles can be significantly improved in the simultaneous acoustic field and supersaturated vapor condensation. High value of sound pressure level (SPL>150dB) was needed to achieve a considerable removal efficiency using acoustic agglomeration only. While low SPL of 130–150dB can be used to remove fine particle efficiently in WFGD system using simultaneous external fields. Removal efficiency higher than 70% was obtained with S=1.15 and SPL=151dB. The residence time of the simultaneous acoustic agglomeration and condensational growth was determined by the time required by acoustic agglomeration. Particle removal efficiency increased with the residence time, but it tended to be constant as the residence time over 3s. Multiple horn numbers with low acoustic intensity benefited the improvement of fine particle removal efficiency and the reduction of energy consumption using the simultaneous external fields in WFGD system.

Nucleation in food colloids.

Nucleation in food colloids has been studied in detail using ultrasound spectroscopy. Our data show that classical nucleation theory (CNT) remains a sound basis from which to understand nucleation in food colloids and analogous model systems using n-alkanes. Various interpretations and modifications of CNT are discussed with regard to their relevance to food colloids. Much of the evidence presented is based on the ultrasound velocity spectrometry measurements which has many advantages for the study of nucleating systems compared to light scattering and NMR due to its sensitivity at low solid contents and its ability to measure true solid contents in the nucleation and early crystal growth stages. Ultrasound attenuation spectroscopy also responds to critical fluctuations in the induction region. We show, however, that a periodic pressure fluctuation such as a quasi-continuous (as opposed to a pulse comprising only a few pressure cycles) ultrasound field can alter the nucleation process, even at very low acoustic intensity. Thus care must be taken when using ultrasound techniques that the measurements do not alter the studied processes. Quasi-continuous ultrasound fields may enhance or suppress nucleation and the criteria to determine such effects are derived. The conclusions of this paper are relevant to colloidal systems in foods, pharmaceuticals, agro-chemicals, cosmetics, and personal products.

Prolonged stimulation with low-intensity ultrasound induces delayed increases in spontaneous hippocampal culture spiking activity.

Ultrasound is a promising neural stimulation modality, but an incomplete understanding of its range and mechanism of effect limits its therapeutic application. We investigated the modulation of spontaneous hippocampal spike activity by ultrasound at a lower acoustic intensity and longer time scale than has been previously attempted, hypothesizing that spiking would change conditionally upon the availability of glutamate receptors. Using a 60-channel multielectrode array (MEA), we measured spontaneous spiking across organotypic rat hippocampal slice cultures (N = 28) for 3 min each before, during, and after stimulation with low-intensity unfocused pulsed or sham ultrasound (spatial-peak pulse average intensity 780 μW/cm2 ) preperfused with artificial cerebrospinal fluid, 300 μM kynurenic acid (KA), or 0.5 μM tetrodotoxin (TTX) at 3 ml/min. Spike rates were normalized and compared across stimulation type and period, subregion, threshold level, and/or perfusion condition using repeated-measures ANOVA and generalized linear mixed models. Normalized 3-min spike counts for large but not midsized, small, or total spikes increased after but not during ultrasound relative to sham stimulation. This result was recapitulated in subregions CA1 and dentate gyrus and replicated in a separate experiment for all spike size groups in slices pretreated with aCSF but not KA or TTX. Increases in normalized 18-sec total, midsized, and large spike counts peaked predominantly 1.5 min following ultrasound stimulation. Our low-intensity ultrasound setup exerted delayed glutamate receptor-dependent, amplitude- and possibly region-specific influences on spontaneous spike rates across the hippocampus, expanding the range of known parameters at which ultrasound may be used for neural activity modulation. © 2016 Wiley Periodicals, Inc.

Design of a low power hybrid HIFU applicator for haemostasis based on acoustic propagation modelling

Purpose: The aim of this study was to design an applicator for haemostasis usage needing lower acoustic intensities (<880 W/cm2) than in previous devices intended for it, which is based on ultrasound propagation FEM modelling using a 2-MHz HIFU transducer. Materials and methods: Acoustic field characterisation and numerical simulations in water were performed with and without the proposed applicator. Parameters such as form factor, ellipsoidal shape ratio, and Euclidean distance were used (among others) to compare simulated data with transducer measurements without applicator. A low density polyethylene cone was manufactured from geometries validated from acoustic field modelling. The hollow cone was filled with 10% polyacrylamide gel as a coupling medium with liver phantom or chicken liver. Focal temperature was measured with a thermocouple embedded in the phantom for 1–20 W driving powers for 120 s. Standing wave ratios (SWR) were used as coupling indexes. Ex vivo experimentation in chicken liver was made at 10–20 W. Results: Simulated acoustic patterns showed good concordance with measurements. Experimental focal distance was 20.72 ± 0.24 mm, while the simulated was 19.79 mm (≈4% error). SWR at low power were: 2.01 with transducer emitting in air, 1.53 at applicator tip, and 1.35 after phantom placement. Average SWR at high power was 1.31. Similarity of percentages for data comparison in focal plane was over 60%. Maximum temperature measured at focus was 88.7 °C with 20 W after 85 s. Conclusions: Temperatures reached at focus suggest that this applicator has good efficiency, which notably reduces the power typically needed for haemostasis effect.

Use of Hydroxyapatite Doping to Enhance Responsiveness of Heat-Inducible Gene Switches to Focused Ultrasound

Recently, we demonstrated that ultrasound-based hyperthermia can activate cells containing a heat-activated and ligand-inducible gene switch in a spatio-temporally controlled manner. These engineered cells can be incorporated into hydrogel scaffolds (e.g., fibrin) for in vivo implantation, where ultrasound can be used to non-invasively pattern transgene expression. Due to their high water content, the acoustic attenuation of fibrin scaffolds is low. Thus, long ultrasound exposures and high acoustic intensities are needed to generate sufficient hyperthermia for gene activation. Here, we demonstrate that the attenuation of fibrin scaffolds and the resulting hyperthermia achievable with ultrasound can be increased significantly by doping the fibrin with hydroxyapatite (HA) nanopowder. The attenuation of a 1% (w/v) fibrin scaffold with 5% (w/v) HA was similar to soft tissue. Transgene activation of cells harboring the gene switch occurred at lower acoustic intensities and shorter exposures when the cells were encapsulated in HA-doped fibrin scaffolds versus undoped scaffolds. Inclusion of HA in the fibrin scaffold did not affect the viability of the encapsulated cells.

Contributions to the intensity field in shallow water waveguides

In a typical waveguide propagation, the acoustic intensity field is made up from contributions by a direct wave, bottom and surface reflected waves, and a number of interface waves. In shallow water environments, the laterally traveling headwave, generated by interaction with a faster propagating bottom layer, may become important. Several numerical techniques are used to calculate intensity fields in shallow water environments including normal modes, parabolic equation, and finite element methodologies. Bottom interactions are modeled with equivalent fluid properties, and the relative influence of the laterally traveling head wave is examined for several bathymetries of interest. Each codes' solution and merits are compared when calculating both propagating (active) and stationary (reactive) low frequency acoustic intensities.