Loch Etive Research Articles

Sediment transport and the freshwater modification of tidal hydraulics approaching a fjordic sill: The Falls of Lora, Loch Etive, western Scotland, UK

AbstractThe Falls of Lora (FoL), Loch Etive, illustrates a highly energetic tidal site across a shallow (mean depth = 8 m) fjordic sill. The FoL hydrodynamics have been investigated using an acoustic Doppler current profiler (ADCP), a numerical flow model, water‐level data and meteorological data. Additionally, four bathymetric timeseries datasets (from February 2014, September 2020, November 2021 and June 2022) provided alternative indication of flow variability by monitoring the geometry of submarine sediment bedforms. A correlation of 0.22 (−0.2 < 97.5% confidence interval < 0.2) was found between catchment‐integrated precipitation and residual water height (with effects of tide and atmospheric pressure removed). Residual water height varied by 0.77 m. Artificially increasing the water height by up to 0.6 m at the model's up‐fjord open boundary to emulate the effects of precipitation increased (decreased) peak depth‐averaged flow speed of the ebb (flood) jet by 0.44 m/s (0.34 m/s) whilst modifying residual flow fields around the FoL. The freshwater‐modified residual flow correlated with the westward displacement of a flow transverse sediment ridge observed in two bathymetric surveys conducted after periods of relatively high precipitation. Additionally, in one of the surveys associated with increased precipitation, wavelength stretching in the sediment dunes was observed uniquely at the western approach to the FoL, dominated by the freshwater‐enhanced ebb flow. Increasing the water levels of the up‐fjord boundary from 0.1 to 0.6 m resulted in an increased mean bedload transport of 2.28 × 10−5 m3/s (370% increase) for these dunes dominated by the ebb current. Whereas Loch Etive has an anomalously high freshwater influence on sill flow compared with other Scottish fjords, it is predicted that freshwater inputs are a significant influence on sill hydrodynamics and nearby sediment transport pathways in other fjordic systems.

Photoperiodism and overwintering in boreal and sub-Arctic Calanus finmarchicus populations

The copepod Calanus finmarchicus, a key species in the North Atlantic, generally spends the non-productive season by descending into deep waters and entering diapause, a physiological state characterized by reduced metabolism and arrested development. In the open ocean, overwintering depths are below 600 m, where temperature and light conditions are favourable to initiate diapause. However, C. finmarchicus has also been reported diapausing in areas with shallow water depth such as fjords, coastal waters and shelf seas. In these environments, the temperature and light conditions are different, and it has been hypothesized that under such conditions C. finmarchicus may remain active throughout winter. Here, we investigated changes in the swimming activity of C. finmarchicus from shallow fjords in the eastern North Atlantic during overwintering in response to ambient photoperiod. We conducted monthly experiments with populations from 2 fjords from different latitudes (sub-Arctic Ramfjord, 69°N and boreal Loch Etive, 56°N), measuring the locomotor activity of individual C. finmarchicus stage CVs exposed to a natural light:dark cycle. At both locations, peaks in activity in response to the light cycle were observed to shift from nocturnal during the early overwintering phase to diurnal during mid and late overwintering phase, with a minimal intensity observed during the mid-overwintering phase. In Ramfjord, activity and rhythmicity were generally lower than in Loch Etive. We conclude that C. finmarchicus remains active throughout its overwintering period when in shallow (<200 m) locations but down-regulates its locomotor activity during the main overwintering phase, which we describe as a winter resting state as distinct from classical diapause.

The chiasmata systems of Scottish Chysolinalatecincta (Demaison, 1896) (Coleoptera, Chrysomelidae).

The meiotic systems of some Scottish individuals of the rare Chrysolinalatecinctassp.intermedia (Franz, 1938) have been analyzed from meiotic cells at diakinesis to study the types of chromosomal bivalents and the number and locations of their chiasmata. The mean number of unichiasmate was about two-thirds and that of bichiasmate bivalents about one-third. Most chiasmata were at distal positions and there were no pairwise statistically significant differences in the mean number of chiasmata and those of unichiasmate and bichiasmate bivalents between the three surveyed geographic sources of these Scottish individuals. However, pairwise significant differences were found in the mean number of proximal + interstitial chiasmata between Loch Etive (Argyllshire) and both Orkney and Shetland Islands individuals. The presumed higher values of genetic recombination due to the proximal + interstitial chiasmata with regard to the prevailing distal ones, might provide a slight selective advantage to the insular individuals against the more extreme climates of both islands compared with the Loch Etive site.

Deposition of artificial radionuclides in sediments of Loch Etive, Scotland

The nuclear fuel reprocessing plants on the Sellafield site (UK) have released low-level effluents into the Irish Sea under authorisation since 1952. This has led to the labelling of nearby offshore sediments with a range of artificial radionuclides. In turn, these sediments act as a long-term secondary source of both soluble and particle-associated radionuclides to coastal areas. These radionuclides are of interest both in assessing possible environmental impacts and as tracers for marine processes. Here we present results from a study of the geochemistry of natural (234, 238U) and artificial (137Cs, 241Am, 238Pu, 239+240Pu, and 236U) radionuclides and their accumulation in sediments from Loch Etive, Scotland. The data are interpreted in the context of the historical radioactive discharges to the Irish Sea and biogeochemical processes in marine sediments. Loch Etive is divided into two basins; a lower, seaward basin where the sedimentation rate (∼0.6 cm/yr) is about twice that of the more isolated upper basin (∼0.3 cm/yr). These accumulation rates are consistent with the broad distribution of 137Cs in the sediment profiles which can be related to the maximum Sellafield discharges of 137Cs in the mid-1970s and suggest that 137Cs was mainly transported in solution to Loch Etive during that period. Enrichments of Mn, Fe, and Mo in sediment and porewater from both Loch Etive basins result from contemporary biogeochemical redox processes. Enrichments of 238U and 234U in the lower basin may be a result of the cycling of natural U. By contrast, the Sellafield-derived artificial isotope 236U does not seem to be affected by the redox-driven reactions in the lower basin. The 238Pu/239,240Pu ratios suggest contributions from both historical Sellafield discharges and global fallout Pu. The uniform sediment distributions of Pu and Am, which do not reflect Sellafield historical discharges, suggest the existence of a homogenous secondary source. This could be the offshore ‘mud patch’ in the vicinity of Sellafield from which the supply of radionuclides reflects time-integrated Sellafield discharges. This source could also account for the continuing supply of Cs to Loch Etive, even after substantial reductions in discharge from the Sellafield site.

Benthic Carbon Mineralization and Nutrient Turnover in a Scottish Sea Loch: An Integrative In Situ Study.

Based on in situ microprofiles, chamber incubations and eddy covariance measurements, we investigated the benthic carbon mineralization and nutrient regeneration in a ~65-m-deep sedimentation basin of Loch Etive, UK. The sediment hosted a considerable amount of infauna that was dominated by the brittle star A. filiformis. The numerous burrows were intensively irrigated enhancing the benthic in situ O2 uptake by ~50 %, and inducing highly variable redox conditions and O2 distribution in the surface sediment as also documented by complementary laboratory-based planar optode measurements. The average benthic O2 exchange as derived by chamber incubations and the eddy covariance approach were similar (14.9 ± 2.5 and 13.1 ± 9.0 mmol m−2 day−1) providing confidence in the two measuring approaches. Moreover, the non-invasive eddy approach revealed a flow-dependent benthic O2 flux that was partly ascribed to enhanced ventilation of infauna burrows during periods of elevated flow rates. The ratio in exchange rates of ΣCO2 and O2 was close to unity, confirming that the O2 uptake was a good proxy for the benthic carbon mineralization in this setting. The infauna activity resulted in highly dynamic redox conditions that presumably facilitated an efficient degradation of both terrestrial and marine-derived organic material. The complex O2 dynamics of the burrow environment also concurrently stimulated nitrification and coupled denitrification rates making the sediment an efficient sink for bioavailable nitrogen. Furthermore, bioturbation mediated a high efflux of dissolved phosphorus and silicate. The study documents a high spatial and temporal variation in benthic solute exchange with important implications for benthic turnover of organic carbon and nutrients. However, more long-term in situ investigations with like approaches are required to fully understand how environmental events and spatio-temporal variations interrelate to the overall biogeochemical functioning of coastal sediments. Electronic supplementary materialThe online version of this article (doi:10.1007/s10498-016-9300-8) contains supplementary material, which is available to authorized users.

Patterns of carbon processing at the seafloor: the role of faunal and microbial communities in moderating carbon flows

Abstract. Marine sediments, particularly those located in estuarine and coastal zones, are key locations for the burial of organic carbon (C). However, organic C delivered to the sediment is subjected to a range of biological C-cycling processes, the rates and relative importance of which vary markedly between sites, and which are thus difficult to predict. In this study, stable isotope tracer experiments were used to quantify the processing of C by microbial and faunal communities in two contrasting Scottish estuarine sites: a subtidal, organic C rich site in Loch Etive with cohesive fine-grained sediment, and an intertidal, organic C poor site on an Ythan estuary sand flat with coarse-grained permeable sediments. In both experiments, sediment cores were recovered and amended with 13C labelled phytodetritus to quantify whole community respiration of the added C and to trace the isotope label into faunal and bacterial biomass. Similar respiration rates were found in Loch Etive and on the Ythan sand flat (0.64 ± 0.04 and 0.63 ± 0.12 mg C m−2h−1, respectively), which we attribute to the experiments being conducted at the same temperature. Faunal uptake of added C over the whole experiment was markedly greater in Loch Etive (204 ± 72 mg C m−2) than on the Ythan sand flat (0.96 ± 0.3 mg C m−2), and this difference was driven by a difference in both faunal biomass and activity. Conversely, bacterial C uptake over the whole experiment in Loch Etive was much lower than that on the Ythan sand flat (1.80 ± 1.66 and 127 ± 89 mg C m−2, respectively). This was not driven by differences in biomass, indicating that the bacterial community in the permeable Ythan sediments was particularly active, being responsible for 48 ± 18 % of total biologically processed C. This type of biological C processing appears to be favoured in permeable sediments. The total amount of biologically processed C was greatest in Loch Etive, largely due to greater faunal C uptake, which was in turn a result of higher faunal biomass. When comparing results from this study with a wide range of previously published isotope tracing experiments, we found a strong correlation between total benthic biomass (fauna plus bacteria) and total biological C processing rates. Therefore, we suggest that the total C-cycling capacity of benthic environments is primarily determined by total biomass.

A geomorphological reconstruction of the deglaciation of Loch Etive during the Loch Lomond Stadial

The Loch Lomond Stadial (LLS) (12.9 – 11.7 ka BP) was the last climatic episode in which Scotland possessed a significant ice mass. Loch and Glen Etive represent one of the best-preserved glacial landsystems in western Scotland, having formed a conduit for ice draining from high on Rannoch Moor. Loch Etive is a glacially over-deepened trough 6 km north of Oban, now a sea loch 30 km long and up to 1.5 km wide, extending from the Firth of Lorn NE towards Rannoch Moor. It is a key site in which to understand the glacial processes that shaped the Western Highlands during the LLS, leading to full glacial retreat by 11.4 ka BP. Based on a comprehensive high-resolution multibeam bathymetric sonar survey, the morphology of the seabed was analysed using ArcGIS to create a series of detailed geomorphological maps. Morphological interpretations, backscatter and seismic reflection data from the loch were combined to reconstruct the deglaciation of Loch Etive. The presence of transverse ridges interpreted as recessional moraines and submarine eskers indicates a dynamic glacial retreat from the maximum seaward extent west of the present-day coastline. At its maximum extent, the glacier formed a proglacial delta generating large volumes of meltwater that flowed over the rock sill of the present-day Falls of Lora. The confluence of glacial ice masses, flowing from the Pass of Brander and tributary corrie glaciers, created a complex glaciofluvial environment in the outer basin of Loch Etive. This is reflected in the formation of a large submarine esker. In the inner loch basin, the presence of recessional moraines at intervals suggests a dynamic glacier, responding to seasonal temperature fluctuations, possibly subsequently retreating northeastwards c. 50 m annually as the LLS ended.

Winter residency and site association in the Critically Endangered North East Atlantic spurdog Squalus acanthias

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 526:113-124 (2015) - DOI: https://doi.org/10.3354/meps11210 Winter residency and site association in the Critically Endangered North East Atlantic spurdog Squalus acanthias J. Thorburn1,*, F. Neat2, D. M. Bailey3, L. R. Noble1, C. S. Jones1 1School of Biological Sciences, College of Life Sciences & Medicine, University of Aberdeen, Aberdeen, AB24 2TZ, UK 2Marine Scotland Science, Aberdeen, AB11 9DB, UK 3Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow, Glasgow, G12 8QQ, UK *Corresponding author: j.thorburn@abdn.ac.uk ABSTRACT: Identification and incorporation of residential behaviour into elasmobranch management plans has the potential to substantially increase their effectiveness by identifying sites where marine protected areas might be used to help conserve species with high migratory potential. There is evidence that the spurdog Squalus acanthias displays site association in some parts of its global distribution, but this has currently not been shown within the North East Atlantic where it is Critically Endangered. Here we investigated the movements of electronically tagged spurdog within Loch Etive, a sea loch on the west coast of Scotland. Archival data storage tags (DSTs) that recorded depth and temperature revealed that 2 mature female spurdog overwintered within the loch, restricting their movements to the upper basin, and remaining either in the loch or the local vicinity for the rest of the year. This finding was supported by evidence for limited movements from conventional mark-recapture data and from an acoustically tagged individual spurdog. Some of the movements between the loch basins appear to be associated with breeding and parturition events. This high level of site association suggests that spatial protection of the loch would aid the conservation of different age and sex classes of spurdog. KEY WORDS: Archival tag · Conservation · Migration · Spatial ecology · Residency · Marine protected areas · Spiny dogfish · Loch Etive Full text in pdf format PreviousNextCite this article as: Thorburn J, Neat F, Bailey DM, Noble LR, Jones CS (2015) Winter residency and site association in the Critically Endangered North East Atlantic spurdog Squalus acanthias. Mar Ecol Prog Ser 526:113-124. https://doi.org/10.3354/meps11210 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 526. Online publication date: April 22, 2015 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2015 Inter-Research.

Dissolved iron(II) ligands in river and estuarine water

We present the first evidence of Fe(II) complexation by natural organic ligands in estuarine waters. Across five diverse river/estuary systems we find evidence of terrestrially derived ligands with binding constants (log KFe(II)L) mainly in the range 6–8. These Fe(II) ligands were stable over short time periods (1–2days), generally equivalent to, or in excess of, ambient freshwater Fe(II) concentrations (which ranged from 12 to 3600nM) and had similar binding constants to ligands that were leached by water from vegetation and detritus (log KFe(II)L 7–8). A class of terrestrially derived ligands may therefore be important in stabilising Fe(II) concentrations in freshwater systems. However, in coastal seawater the impact of these ligands upon Fe(II) speciation is likely to be diminished due to a combination of dilution, loss of humic material during flocculation and increased ionic strength.The temperate and sub-tropical river systems studied included the Beaulieu (England), Itchen (England), Cape Fear (North Carolina, USA), Winyah Bay (South Carolina, USA) and Loch Etive (Scotland). Freshwaters in each system possessed a broad range of dissolved organic carbon (DOC, 200–1300μM), labile dissolved Fe (LDFe, Fe<0.2μm available to ferrozine after reduction with ascorbic acid, 100nM–20μM) and pH (5.5–8.5). In the Itchen estuary, where anthropogenic discharge constitutes >10% of freshwater input, ligand binding constants were elevated (up to log KFe(II)L 11) and the expected decrease in LDFe with increasing salinity along the estuary was not observed (LDFe and DOC both peaked at a salinity of 7) due to effluent inputs.

Using the radium quartet (228Ra, 226Ra, 224Ra, and 223Ra) to estimate water mixing and radium inputs in Loch Etive, Scotland

The radium (Ra) quartet (228Ra, 226Ra, 224Ra, and 223Ra) has been investigated in Loch Etive, a Scottish fjord, to provide new constraints on water mixing rates and on the inputs of Ra from sediments. Maximum water transport rates for the inflowing estuarine layer at 5 m depth, determined from the excess 223Ra (223Raex), indicate that this water travels at no more than 2.4 ± 0.2 cm s−1 net and that it takes 17 ± 2 d for waters to travel from the mouth to the head of the loch if no horizontal mixing is taken into account. Alternatively, neglecting advection, the short‐lived Ra distribution could be explained by horizontal mixing rates of 6.1 × 106 cm2 s−1 (223Raex) or 9.1 × 106 cm2 s−1 (224Raex). Periodic overturning circulation plays an important role in resetting chemical cycles in the isolated deep basin of the inner loch. Sediment in this deep basin provides the major input of 228Ra to the isolated deep water, and the accumulation of 228Ra in deep waters allows an assessment of sedimentary fluxes of 228Ra, a poorly constrained aspect of the 228Ra input to the global ocean. The calculated sedimentary 228Ra flux of 2.1 ± 0.2 (× 109) atoms m−2 yr−1 in the inner deep basin is comparable with previous measurements of sedimentary 228Ra inputs from shelf sediments, supporting existing global 228Ra budgets, which are used to assess global rates of groundwater discharge to the ocean.

Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment: An investigation of possible mediators of diapause

The marine copepod, Calanus finmarchicus, is a crucial component of the pelagic food web in the North Atlantic and peripheral seas where it is a major player in biogeochemical cycles and the productivity of commercially important fisheries. A key stage in its life cycle is the emergence of the pre-adult, copepodite developmental stage five (CV) from a period of overwintering dormancy, known as diapause. As is the case in many insect species, diapause is also likely to be under endocrine control in C. finmarchicus. To investigate the hormonal regulation of diapause behaviour of stage CV C. finmarchicus, the expression of three key genes: ecdysone receptor (EcR), retinoid X receptor (RXR) and an A-type allatostatin (A-type AST), were measured in specimens collected at monthly intervals from Loch Etive, a ca. 150m deep sea loch on the west coast of Scotland, between June 2006 and May 2007. The full length RXR gene was cloned and sequenced from C. finmarchicus, and was found to share 49–53% total identity with equivalent genes encoding proteins from other crustaceans, and >80% identity in the DNA binding domain with other crustaceans, insects and vertebrates. EcR expression was least in December when the animals are expected to be in diapause, but began to increase in January, when the animals were terminating diapause. Concomittant with the rise in EcR in January was low expression of A-type AST and high expression of RXR.

A box model of the seasonal exchange and mixing in Regions of Restricted Exchange: Application to two contrasting Scottish inlets

We present a time-dependent box model of exchange and mixing processes in Regions of Restricted Exchange (RREs). The model is applied to two contrasting Scottish inlets, but can potentially be applied to a wide range of stratified inshore water bodies. The model represents the vertical structure of the inlet using up to three horizontally uniform layers, representing surface, intermediate and deep basin water respectively, and calculates the daily volume, thickness, salinity and temperature of each layer over an annual cycle. The model is forced by observed time series of daily-mean wind stress, river discharge, surface heat flux, and depth-profiles of the external coastal temperature and salinity. Advective and diffusive fluxes between the layers, and between the inlet and adjacent coastal ocean, are calculated from parameterisations of known physical processes in inshore stratified waters, utilising published analytical solutions and empirically-based formulae. The goal is for the model to be generally applicable to RREs to support improved resource management. As such, the number of free parameters within the model has been kept to a minimum.The model predictions of layer temperature and salinity are quantitatively compared to observations from year-long sampling programmes in two contrasting Scottish inlets, Loch Creran and Loch Etive. The model showed good agreement with the observed data, reproducing the broad scale seasonal cycle and other shorter-term fluctuations. RMS errors for temperature were less than 1 °C, and for salinity typically of the order 1. The model results suggest that Loch Creran behaved as a well-mixed box, with weak stratification maintained by strong vertical diffusion, and volume exchange between the inlet and coastal waters dominated by tidal forcing. In contrast, Loch Etive exhibited classical fjordic three-layer hydrography and dynamics, with strong stratification, weak vertical diffusion and a density-driven circulation comparable in strength to tidal exchange. The effects of a deep water renewal event on water properties were successfully reproduced by the model. The potential for wider application of the model to resource management issues and to other types of RRE is discussed.

The Dalradian rocks of the central Grampian Highlands of Scotland

The central Grampian Highlands, as defined here, are bounded to the north-west by the Great Glen Fault, to the south-west by Loch Etive and the Pass of Brander Fault and to the south-east by the main outcrop of the Loch Tay Limestone Formation. The more arbitrary northern boundary runs north-west along the A9 road and westwards to Fort William. The detailed stratigraphy of the Dalradian Supergroup ranges from the uppermost Grampian Group through to the top of the Argyll Group, most notably seen in the two classic areas of Loch Leven–Appin and Schiehallion–Loch Tay; Southern Highland Group strata are preserved only in a small structural inlier south of Glen Lyon.Major F1 and F2 folds are complicated by co-axial northeast-trending F3 and F4 folding, as well as by locally important north- or NW-trending folds. In the Loch Leven area, nappe-like F1 folds verge to the north-west, whereas to the south-east the major recumbent F1/F2 Tay Nappe verges to the south-east. The trace of the upright Loch Awe Syncline lies between the opposing nappes, but in this region a large mass of late-Caledonian granitic rocks obscures their mutual relationship. Three tectonic ‘slides’ are identified that are certainly zones of high strain but which in part could be obscuring stratigraphical variations.The regional metamorphism ranges from greenschist facies on the western seaboard of Argyll to amphibolite facies in most of the remainder of the region. The study of garnets, together with kyanite and staurolite in the Schiehallion area, has enabled a detailed history of the metamorphism and structure to be unravelled.Stratabound mineralization occurs in the Easdale Subgroup, where there is also evidence of changes of sedimentary environment associated with volcanicity and lithospheric stretching. The region is dissected by a series of NE-trending, dominantly left-lateral, faults, subparallel to the Great Glen Fault, whose movement history is illustrated here by that of the Tyndrum Fault.

Composition of wax esters is linked to diapause behavior of Calanus finmarchicus in a sea loch environment

We studied the seasonal lipid dynamics of Calanus finmarchicus from Loch Etive, a semi‐enclosed fjordic environment on the west coast of Scotland, in relation to the diapause behavior of this species. Levels of total lipid and triacylglycerol in individual copepodid stage V (CVs) exhibited clear seasonal trends, with highest levels in June and progressively decreasing through late summer to winter. Wax esters in CVs were more variable, but followed a similar pattern. An index of the level of unsaturation of wax esters, effectively an indicator of the levels of polyunsaturated fatty acids in the wax ester reserves, were highest in midsummer and declined progressively through autumn and winter. Fatty acid analysis of total lipid in the CVs confirmed that the amounts of polyunsaturated fatty acids in the wax esters decreased, while amounts of saturated and monounsaturated fatty acids remained comparatively stable. Polyunsaturated wax esters are selectively catabolized by C. finmarchicus during diapause. Previous studies have linked unsaturation of wax esters in calanoid copepods to the initiation of diapause and buoyancy control, and we hypothesize that selective catabolism of polyunsaturated wax esters is linked to the processes of diapause termination and ascent to surface waters.

Comparison of three different methods for assessing in situ friction velocity: A case study from Loch Etive, Scotland

Three approaches, Eddy Correlation (EC), Turbulent Kinetic Energy (TKE), and Inertial Dissipation (ID) methods, were compared to evaluate their potential for estimation of friction velocity in a Scottish sea loch. As an independent assessment parameter, we used simultaneous O2 recordings of the diffusive boundary layer (DBL) that were compared with theoretical distribution as derived from the respective friction velocity estimates. Friction velocities were calculated based on the three approaches using continuously measured turbulent properties, and values estimated by the TKE method were significantly higher than values of the two other approaches. Time series of calculated friction velocity were subsequently employed as input parameters for nonsteady calculations of the O2 distribution within the DBL. The friction velocity is a controlling factor for the eddy diffusivity distribution immediately above the sediment surface and for the DBL thickness, and friction velocity values of the TKE method derived significantly higher theoretical O2 concentration while the EC and the ID approach provided results that were not significantly different. Overall differences from measured O2 concentration in the DBL were 0.2% for the EC method, 9.8% for the TKE method, and 0.7% for the ID method. The results reveal that the EC method appear to be the best approach for estimating friction velocities though not significantly different from the ID method, whereas the TKE method was unreliable at the ~70 m deep, relatively calm study site. This information is important for future research in similar sounds, fjords, and sea‐lochs.

Quantitative microbial source apportionment as a tool in aiding the identification of microbial risk factors in shellfish harvesting waters: the Loch Etive case study

Special Issue on proceedings of the Scottish Aquaculture: A Sustainable Future? Symposium, 21-22 April 2009, Edinburgh

Scottish Mytilus trossulus mussels retain ancestral mitochondrial DNA: Complete sequences of male and female mtDNA genomes

Mytilus trossulus mussels occur in North America and in the Baltic Sea. Recently genetic markers for the three Mytilus subspecies M. edulis, M. galloprovincialis, and M. trossulus, have been detected at Loch Etive in Scotland suggesting mixed ancestry for this population. Of particular interest is the evidence that M. trossulus occurs at Loch Etive because it had not previously been reported in the British Isles. In the present study, analysis of subspecies-specific diagnostic nuclear DNA markers confirms the presence of a high frequency of mussels with M. trossulus ancestry at Loch Etive. The genetic structure suggests hybridisation at an intermediate stage compared with North American populations, where there is little hybridisation, and Baltic populations where there is extensive introgression. This points strongly against a Baltic origin for Loch Etive M. trossulus. The F and M mitochondrial DNA (mtDNA) genomes of Baltic M. trossulus are similar in sequence to the corresponding genomes in M. edulis and believed to be derived by introgression from that subspecies. Both F and M mtDNA genomes are observed at Loch Etive consistent with the presence of doubly uniparental inheritance. Here we provide the complete sequences of the three M. trossulus mtDNA genomes (one F and two M) from Loch Etive. These genomes are extremely similar to the corresponding genomes from ancestral M. trossulus in America but divergent from the genomes for Baltic M. trossulus. This is the first report of ancestral M. trossulus mtDNA genomes in Europe. The F and M genomes are diverged by 26% in nucleotide sequence, similar to other Mytilus F and M genomes. The gene arrangement in the sequenced genomes is also similar to that in other sequenced Mytilus mtDNA genomes. However the two sequenced M genomes differ by 960 bp which is caused by a duplication in the main noncoding region (CR). This duplication has not so far been observed in North American populations of M. trossulus. The coding regions of the Loch Etive genomes have no features suggesting that they are other than functional genomes and have K a/ K s values in coding regions less than one indicative of purifying selection. Estimates of divergence times were made for both genomes and are consistent with invasion of Loch Etive by M. trossulus towards the end of the last glacial period.

Pollen analysis of Holocene sediments from Loch Etive, a Scottish fjord

Abstract This paper outlines one of the first attempts to analyse the detailed pollen content of the marine sediments from a fjord (sea loch) located on the west coast of Scotland. The site chosen was in the Bonawe Deep, part of the upper basin of Loch Etive, and 2.3 m depth of sediments were sampled. These sediments were examined for their fossil pollen content and a pollen diagram was constructed from the results. The interpretation of the pollen record was supported by seven radiocarbon dates which demonstrated that the sediments accumulated over the last 8–9 ka of the Holocene. The pollen diagram was divided into three pollen assemblage zones. The lowest zone ET1 ( c . 9–7.3 ka BP) was dominated by pollen of Betula , Pinus and Corylus , zone ET2 ( c . 7.3–1.2 ka BP) contained a wide range of woodland pollen taxa (mainly Betula , Pinus , Quercus , Alnus and Corylus ) and the uppermost zone ET3 (1.2–0 ka BP) was distinguished by fewer tree pollen taxa and increased values of open habitat taxa, particularly Calluna . The pollen diagram showed the development of vegetation during the Holocene which correlated well with existing results from studies of peat or freshwater lake sediments sampled in the west of Scotland. The only significant differences were the higher concentrations of Pinus pollen found in the Loch Etive site. These differences can be explained by the large catchment area of the loch which includes high ground, where it is proposed that extensive Pinus forest grew during much of the Holocene. The study demonstrates the practicality of using Scottish fjord sediments for Holocene pollen studies and potentially provides a link between terrestrial vegetation records and marine-based fossil records.

The effects of large and small-scale topography upon internal waves and implications for tidally induced mixing in sill regions

A free surface non-hydrostatic model in a cross-sectional form, namely, two-dimensional, in the vertical is used to examine the role of larger-scale topography, namely, sill width, and smaller scale topography, namely, ripples on the sill upon internal wave generation and mixing in sill regions. The present work is set in the context of earlier work and the wider literature in order to emphasise the problems of simulating mixing in hydrographic models. Highlights from previous calculations and references to the literature for detail, together with new results presented here with smooth and “ripple” topography, are used to show that an idealised cross-sectional model can reproduce the dominant features found in observations at the Loch Etive sill. Calculations show that on both the short and long time scales, the presence of small-scale “ripple” topography influence the mixing and associated Richardson number distribution in the sill region. Subsequent calculations in which the position and form of the small-scale sill topography is varied show for the first time that it is the small-scale topography near the sill crest that is particularly important in enhancing mid-water mixing on the lee side of the sill. Both short-term and longer-term calculations with a reduced sill width and associated time series show that as the sill width is reduced, the non-linear response of the system increases. In addition, Richardson number plots show that the region of critical Richardson number, and hence enhanced mixing, increases with time and a reduction in sill width. Calculations in which buoyancy frequency N varies through the vertical show that buoyancy frequency close to the top of the sill is primarily controlling mixing rather than its mean value. Hence, a Froude number based on sill depth and local N is the critical parameter rather than one based on total depth and mean N.

Gametogenic asynchrony of mussels Mytilus in a mixed-species area: Implications for management

Marine mussels Mytilus edulis, Mytilus galloprovincialis, Mytilus trossulus and their hybrids have recently been reported to occur sympatrically in both wild and farmed populations in Scotland. The presence of M. trossulus has led to significant economic losses at some aquaculture sites. Interest in understanding the reproductive cycles of these mussels in a mixed-species aquaculture area, and the potential to use this information to favour cultivation of the preferred species M. edulis, has motivated a seasonal study of the maturation stages of M. edulis, M. trossulus and M. trossulus × M. edulis hybrids, the most frequent genotypes at one site in Loch Etive, Scotland. To investigate larval abundance of these genotypes, plankton samples were collected simultaneously at the same site over the year and analysed using a real-time PCR assay for the identification of Mytilus species-specific alleles. The main spawning events of M. trossulus and M. trossulus × M. edulis hybrids occurred slightly later, but also more frequently and over a longer period than those of M. edulis. M. trossulus alleles were detected in plankton samples throughout the year, always in larger numbers than M. edulis alleles, except during February. Overall, results suggest that the settlement of M. trossulus and/or M. trossulus × M. edulis hybrids may occur during most of the year. This implies that management of the timing of the deployment of settlement ropes to significantly favour the settlement and production of M. edulis is unlikely to be successful. The real-time PCR methodology has provided valuable information on the temporal patterns of abundance of alleles of each species at the planktotrophic stage of development, and demonstrated the potential to identify and quantify Mytilus larvae in plankton samples.