Listeria Monocytogenes Research Articles

SUBACUTE TOXICITY OF AN AMOXICILLIN-BASED ANTIBACTERIAL PREPARATION IN PIGS UPON REPEATED ADMINISTRATION

The article presents the results of a study of the subacute toxicity of the veterinary drug «Amoksidev 60» in pigs under conditions of repeated administration. «Amoksidev 60» is an antibacterial veterinary drug for systemic use, the active ingredient of which is amoxicillin. Amoxicillin is a semi-synthetic antibiotic from the group of aminopenicillins. It is active against gram-positive (Staphylococcus spp., Streptococcus spp., Actinomyces spp., Clostridium spp., Erysipelothrix rhusiopathiae, Listeria monocytogenes, Bacillus anthracis, Corynebacterium spp.) and some gram-negative microorganisms (Salmonella spp., Pasteurella spp., Haemophilus spp., Actinobacillus spp., Bordetella bronchiseptica, E. coli, Fusobacterium spp., Moraxella spp., Proteus mirabilis). Amoxicillin is not effective against strains that produce beta-lactamase. The mechanism of action of amoxicillin is to disrupt the formation of the cell wall of vegetative forms of bacteria by irreversible blocking of penicillin-binding proteins (PBPs) – enzymes carboxypeptidases and transpeptidases. The drug is used to treat poultry and pigs for diseases of the digestive tract and respiratory organs caused by microorganisms sensitive to amoxicillin. Oral administration of the drug «Amoksidev 60» (based on amoxicillin) to piglets with drinking water at doses of 20,0 and 100,0 mg/kg body weight for 10 days does not cause hemo-, hepato- and nephrotoxic effects on the target animals under the conditions of a subacute toxicological experiment. However, when the drug was administered at a dose of 200,0 mg/kg body weight 10 days after the start of the experiment, a decrease in the level of total hemoglobin by 6,4%, hematocrit by 5,9%, and the number of erythrocytes by 13,4% (p<0,05) was recorded in the blood of pigs. At the same time, an increase in the activity of ALT, AST and urea concentration by 25,7%, 21,6% and 10,2% (p<0,05), respectively, was noted in the blood serum. However, 7 days after the cessation of administration of the drug «Amoksidev 60» with drinking water in a 10-fold dose, hematological and biochemical blood parameters of pigs did not differ from control values. The prospects of further research will be focused on the analysis of the long-term effect of «Amoksidev 60» on the animal body. In particular, it is planned to investigate possible long-term toxic effects with prolonged use of the drug. In addition, an assessment of the cumulative effect during repeated courses of treatment is provided.

From cotton gin byproduct to nano-in-nano structured hybrid composite for effective pathogen control

Cotton gin byproduct (CGB), a high-volume byproduct of cotton processing composed of discarded plant materials, is often underutilized despite its abundance and potential value. In this study, CGB was upcycled into a nano-in-nano structured composite with potent antimicrobial properties, offering a sustainable solution for agricultural waste management and pathogen control. Cellulose nanofibers (CNF) were extracted from CGB and used as an active biotemplate for the in situ synthesis of antimicrobial silver nanoparticles (Ag NPs). CNF inherently facilitated the formation of Ag NPs (17.2 ± 4.7 nm, 6.8 wt%) without the need for external reducing or stabilizing agents, resulting in a hybrid nanocomposite (Ag-CNF). This embedded nanostructure demonstrated stability and potent antimicrobial efficacy, achieving >99.99% reduction of S. Typhimurium and Listeria monocytogenes within 10 min, Pseudomonas aeruginosa within 1 h, and Staphylococcus aureus within 24 h at a 0.5 wt% concentration. These findings suggest the potential of Ag-CNF as an eco-friendly antimicrobial material with applications in disease prevention and sustainable waste utilization.

From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes

Peptidoglycan hydrolases are promising alternatives for combating pathogens due to their specificity and potent bacteriolytic activity. In this study, a novel M23 peptidase from Streptococcus thermophilus NCTC10353, designated StM23, was discovered and characterized. It exhibited antibacterial activity against Listeria monocytogenes and other Gram-positive bacteria with meso-DAP-type peptidoglycan, including Bacillus subtilis and Bacillus cereus. To enhance StM23’s efficacy and specificity, a chimeric enzyme, StM23_CWT, was engineered by fusing its catalytic domain with a cell wall-targeting domain (CWT) from SpM23B, a peptidoglycan hydrolase found in Staphylococcus pettenkoferi. The engineered chimera demonstrated expanded specificity, showing activity against Staphylococcus aureus and Enterococcus faecium. Its ability to disrupt L. monocytogenes cells was visualized by electron microscopy. The enzyme effectively disrupted biofilm structures and decontaminated surfaces like glass, stainless steel, and silicone, showcasing its industrial potential. Safety evaluations using zebrafish, moth larvae, and human cell models confirmed its non-toxic profile, supporting its broad applicability. Based on these findings, StM23_CWT is a novel and potent antimicrobial agent with significant potential to reduce the risk of listeriosis and control persistent pathogens.

The Effect of Muicle–Chitosan Edible Coatings on the Physical, Chemical, and Microbiological Quality of Cazon Fish (Mustelus lunulatus) Fillets Stored in Ice

Fishery products are highly perishable; therefore, effective preservation strategies are essential to maintain their freshness, quality, and shelf life. One promising approach involves the use of edible coatings formulated with natural extracts, such as muicle (Justicia spicigera). This study evaluated the effect of a muicle–chitosan edible coating on the physical, chemical, and microbiological quality of cazon fish (Mustelus lunulatus) fillets stored in ice for 18 days. The muicle extract was obtained by macerating dried leaves for 48 h, and its antibacterial activity was subsequently assessed. A control group (C) and three treatments—muicle extract (ME), chitosan (CH), and a combined muicle–chitosan coating (MECH)—were applied and monitored throughout the storage period. Quality parameters, including pH, colour, water-holding capacity (WHC), texture, total volatile basic nitrogen (TVB-N), and mesophilic microbial counts, were evaluated. The muicle extract exhibited antibacterial activity, with MIC and IC50 values of 3.01 ± 0.73 and 204.56 ± 20.23 µg/mL against Shewanella putrefaciens, and 0.10 ± 0.07 and 118.09 ± 14.51 µg/mL against Listeria monocytogenes, respectively. Treatments of ME, CH, and MECH significantly improved (p < 0.05) the quality of fillets by reducing TVB-N, pH, and microbial load compared to the control. In conclusion, the muicle extract demonstrated antibacterial potential and, either alone or in combination with chitosan, effectively preserved the physical, chemical, and microbiological quality of cazon fillets during ice storage.

A novel duplex qPCR-HRMA technique for simultaneous detection of Listeria monocytogenes and Salmonella typhimurium in meat products.

A novel duplex qPCR-HRMA technique for simultaneous detection of Listeria monocytogenes and Salmonella typhimurium in meat products.

Lactic Acid Bacteria Synergy: Electrostatic Spraying, Dipping, and Formulation Applications of Lactobacillus salivarius L28 and Enterococcus faecium J19 to Enhance Cheese Safety Against Listeria monocytogenes.

Lactic Acid Bacteria Synergy: Electrostatic Spraying, Dipping, and Formulation Applications of Lactobacillus salivarius L28 and Enterococcus faecium J19 to Enhance Cheese Safety Against Listeria monocytogenes.

Application of phage-derived enzymes for enhancing food safety.

Application of phage-derived enzymes for enhancing food safety.

Characterization of hemp (Cannabis sativa L.) raw bast fibers of monoecious versus dioecious cultivars from flowering to senescence: FTIR of lignocelluloses and extract bioactivities.

Characterization of hemp (Cannabis sativa L.) raw bast fibers of monoecious versus dioecious cultivars from flowering to senescence: FTIR of lignocelluloses and extract bioactivities.

Assessing water fluorescence as a rapid predictor for chlorine disinfection of Escherichia coli O157:H7 and Listeria monocytogenes in fresh produce wash water

Assessing water fluorescence as a rapid predictor for chlorine disinfection of Escherichia coli O157:H7 and Listeria monocytogenes in fresh produce wash water

Evaluation of the activity and molecular characterisation of bacteriocins produced by E. faecium and E. faecalis isolated from different hosts against public health-threating pathogens.

Evaluation of the activity and molecular characterisation of bacteriocins produced by E. faecium and E. faecalis isolated from different hosts against public health-threating pathogens.

Xenopus laevis as an infection model for human pathogenic bacteria.

Animal infection models are essential for understanding bacterial pathogenicity and corresponding host immune responses. In this study, we investigated whether juvenile Xenopus laevis could be used as an infection model for human pathogenic bacteria. Xenopus frogs succumbed to intraperitoneal injection containing the human pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Listeria monocytogenes. In contrast, non-pathogenic bacteria Bacillus subtilis and Escherichia coli did not induce mortality in Xenopus frogs. The administration of appropriate antibiotics suppressed mortality caused by S. aureus and P. aeruginosa. Strains lacking the agr locus, cvfA (rny) gene, or hemolysin genes in S. aureus, LIPI-1-deleted mutant of L. monocytogenes, which attenuate virulence within mammals, exhibited reduced virulence in Xenopus frogs compared with their respective wild-type counterparts. Bacterial distribution analysis revealed that S. aureus persisted in the blood, liver, heart, and muscles of Xenopus frogs until death. These results suggested that intraperitoneal injection of human pathogenic bacteria induces sepsis-like symptoms in Xenopus frogs, supporting their use as a valuable animal model for evaluating antimicrobial efficacy and identifying virulence genes in various human pathogenic bacteria.

Green synthesis of silver-modified bacterial cellulose with enhanced antimicrobial activity for advanced biomedical application.

Green synthesis of silver-modified bacterial cellulose with enhanced antimicrobial activity for advanced biomedical application.

How to sample Listeria monocytogenes culture for volatolomics by headspace extraction-gas chromatography-Q Exactive-Orbitrap mass spectrometry?

How to sample Listeria monocytogenes culture for volatolomics by headspace extraction-gas chromatography-Q Exactive-Orbitrap mass spectrometry?

Real-time Detection of Foodborne Pathogens and Biofilm in the Food Processing Environment with Bactiscan, A Macro-scale Fluorescence Device.

Real-time Detection of Foodborne Pathogens and Biofilm in the Food Processing Environment with Bactiscan, A Macro-scale Fluorescence Device.

Genomic diversity and distribution of Listeria monocytogenes strains isolated from imported and national fresh produce in Mexico from 2014 to 2018.

Genomic diversity and distribution of Listeria monocytogenes strains isolated from imported and national fresh produce in Mexico from 2014 to 2018.

Formulation and biological evaluation of sodium alginate-based films blended with watercress oil: A Promising solution for combating foodborne pathogens and potential food packaging applications.

Formulation and biological evaluation of sodium alginate-based films blended with watercress oil: A Promising solution for combating foodborne pathogens and potential food packaging applications.

Comparative analysis of virulence in Listeria monocytogenes: Insights from genomic variations and in vitro cell-based studies.

Comparative analysis of virulence in Listeria monocytogenes: Insights from genomic variations and in vitro cell-based studies.

Identification and characterization of a novel bacteriocin produced by Lactiplantibacillus pentosus and the antibacterial mechanism on Listeria monocytogenes.

Identification and characterization of a novel bacteriocin produced by Lactiplantibacillus pentosus and the antibacterial mechanism on Listeria monocytogenes.

Survival of Salmonella Typhimurium, Listeria monocytogenes, and their surrogates on cantaloupes after triple-washing with peroxyacetic acid

Survival of Salmonella Typhimurium, Listeria monocytogenes, and their surrogates on cantaloupes after triple-washing with peroxyacetic acid

Listeria monocytogenes isolated on selective chromogenic plating media necessitates further characterization to confirm its presence in dairy foods

Listeria monocytogenes isolated on selective chromogenic plating media necessitates further characterization to confirm its presence in dairy foods