Lipid Peroxidation In Rat Liver Research Articles

Inhibitory effect of Solanum anguivi fruit extracts against lead-induced lipid peroxidation in rat liver and kidney

Rapid incidence of liver and kidney disorders resulting in high mortality nowadays is of great concern and there is this very need to salvage the sudden death resulting from the disorders. Lipid peroxidation which is the hallmark of oxidative stress that damage body tissues is prominent as one of the causative factors leading to many chronic and degenerative diseases. This study is sought to investigate the inhibitory effect of aqueous and ethanolic extracts of Solanum Anguivi fruit against lead acetate induced lipid peroxidation in the liver and kidney tissues of albino rats in vitro using thiobarbituric acid reactive substances (TBARS) assay. The results obtained indicate a concentration dependent increase in inhibitory potential of the extracts against lead acetate in both liver and kidney tissues. Highest extract inhibitory potential was (90%) at highest extract concentration (160mg/ml) and this was obtained in the liver tissue. Hence, the results from this study suggest that oxidative stress in liver and kidney tissues could be potentially prevented or managed by dietary intake of Solanum Anguivi fruits.

Evaluation of Antioxidant Potential of Asystasia Chelonoides var.chelonoides Nees.(Acanthaceae)

The ethanolic leaf extract of Asystasia chelonoides var. chelonoides Nees. was evaluated using various in vitro and ex vivo assays. Evaluations were made for DPPH radical scavenging activity, NO radical scavenging activity, Super oxide radical scavenging activity ,Ferric Reducing Antioxidant Potential (FRAP) assay, Total antioxidant activity , Reducing Power activity and Lipid Peroxidation inhibition. Over all findings revealed that A.chelonoides leaf exhibited four extracts namely (AC HEX, AC CHL, AC ETH and AC CRD) and were selected for the study, AC ETH and AC CRD showed potent antioxidant activity by inhibiting DPPH radical in a concentration dependent manner . The nitric oxide radical scavenging activity of all the samples increased as their concentration increased from 20 µg/mL to 320 µg/ mL. AC CRD showed its maximum superoxide radical scavenging of 78.56 % at 320 µg/mL and the EC50 was found to be 93.26 µg/mL. The extracts exhibited a dose dependent reducing power activity within the applied concentrations (20 µg/mL to 100 µg/mL) and AC CRD at 100 µg/mL concentration showed highest activity, which was almost comparable to standard ascorbic acid. In FRAP assay the crude extract of A. chelonoides (AC CRD ) showed 138.14 µg trolox equivalent/g of dry extract respectively. Total antioxidant activity of AC CRD, AC HEX, AC CHL and AC ETH were found to be 98.27, 14.86, 20.16 and 72.56 µg of ascorbic acid equivalent/mg of dry extract respectively. A.chelonoides crude extract and ethanolic fraction showed potent inhibition of FeCl2AA stimulated rat liver lipid peroxidation in concentration dependent manner.

Effect of a parenteral fish-oil-containing lipid emulsion on liver lipid perioxidation and antioxidative defenses in Lewis rats.

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), naturally abundant in fish oil (FO), are known for their anti-inflammatory and potential antioxidant properties. The aim in this article is to evaluate the effect of the infusion of a parenteral FO-containing lipid emulsion on markers of liver lipid peroxidation and oxidative stress in rats undergoing central venous catheterization (CVC). After 5-day acclimatization, adult Lewis rats (n = 42) receiving a 20-g/day AIN-93M oral diet were randomly subdivided into four groups: (1) basal control (BC) (n = 6), without CVC or LE infusion; (2) SHAM (n = 12), with CVC but without LE infusion; (3) soybean oil (SO)/medium-chain triglyceride (MCT) (n = 12), with CVC and receiving LE without FO (4.3 g/kg fat); and (4) SO/MCT/FO (n = 12), with CVC and receiving LE containing 10% FO (4.3 g/kg fat). Animals from the BC group were euthanized immediately after acclimatization. The remaining groups of animals were euthanized after 48 or 72 h of surgical follow-up to assess profiles of liver and plasma fatty acids by gas chromatography, liver gene transcription factor Nrf2, F2-isoprostane lipid peroxidation biomarker, and the antioxidant enzymes glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) by enzyme-linked immunosorbent assay. R program (version 3.2.2) was utilized for data analysis. Compared with the other groups, liver EPA and DHA levels were higher in the SO/MCT/FO group, which also showed the highest liver Nrf2, GPx, SOD, and CAT levels and lower liver F2-isoprostane (P < 0.05). Experimental delivery of FO via EPA and DHA sources in a parenteral LE was associated with a liver antioxidant effect.

Salvia hispanica L. (chia) seed ameliorates liver injury and oxidative stress by modulating NrF2 and NFκB expression in sucrose-rich diet-fed rats.

The aim of this study was to analyze the liver injury and oxidative stress in an experimental model of Metabolic Syndrome (MS) induced by chronic administration of a sucrose-rich diet (SRD) and to evaluate the effects of chia seed as a therapeutic strategy. Male Wistar rats were fed with a reference diet (RD) -6 months- or a SRD -3 months. Then, the latter group was randomly divided into two subgroups. One subgroup continued receiving the SRD for up to 6 months and the other was fed with a SRD where whole chia seed was incorporated as a source of dietary fat for the next 3 months (SRD+CHIA). The results showed that rats fed with a SRD for a long period of time developed dyslipidemia, hyperglycemia, hepatic lipid accumulation, liver injury, hepatic lipid peroxidation and oxidative stress. Hepatic NrF2 expression was significantly decreased. In addition, a significant increase in hepatic NFκB p65 expression and a positive correlation of this with plasma TNFα levels were found. The administration of chia seed for 3 months reversed dyslipidemia, hyperglycemia, lipid accumulation, liver injury, lipid peroxidation and oxidative stress. In the liver tissue, NrF2 expression was normalized and NFκB p65 expression was decreased, the latter was associated with a decrease in plasma TNFα levels. The present study showed new aspects of liver damage, lipid peroxidation and oxidative stress in dyslipidemic insulin resistant rats chronically fed with a sucrose-rich diet. However, we demonstrated new properties and molecular mechanisms associated with the beneficial anti-oxidant effects of chia seed consumption.

In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes

In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PTX was assessed using MCF7, Hela, and HUVEC cell lines. In order to evaluate the in vivo efficacy of niosomal PTX in rats as compared to free PTX, the animals were intraperitoneally administered with 2.5 mg/kg and 5 mg/kg niosomal PTX for two weeks. Results showed that the pH-responsive niosomes had a nanometric size, spherical morphology, 77% EE, and pH-responsive release in pH 5.2 and 7.4. Compared with free PTX, we found markedly lower IC50s when cancer cells were treated for 48 h with niosomal PTX, which also showed high efficacy against human cancers derived from cervix and breast tumors. Moreover, niosomal PTX induced evident morphological changes in these cell lines. In vivo administration of free PTX at the dose of 2.5 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. The situation was different when niosomal PTX was administered to the rats: the 5 mg/kg dosage of niosomal PTX significantly increased serum biochemical parameters, but the group treated with the 2.5 mg/kg dose of niosomal PTX showed fewer toxic effects than the group treated with free PTX at the same dosage. Overall, our results provide proof of concept for encapsulating PTX in niosomal formulation to enhance its therapeutic efficacy.

Synthesis, characterization, toxicity and morphology assessments of newly prepared microemulsion systems for delivery of valproic acid

Valproic acid (VPA), an anti-epileptic drug used to treat seizures, epilepsy, and psychological disorders, has shown promising antitumor potential; however, the side effects are a serious concern. This effort reports the nano-formulation and characterization of oil-in-water F127/lignin microemulsions to enhance the bioavailability of valproic acid (VPA). Microemulsions showed a high encapsulation efficiency and prolonged release. F127/VPA and lignin/VPA microemulsions were tested for their cytotoxic activity via MTT colorimetric assay and LDH leakage assay in cancerous (MCF7 and Hela) and normal (HUVEC) cell lines. High encapsulation efficiency and sustained release were observed for F127/VPA microemulsions. Cell proliferation assays showed that VPA microemulsions induced evident morphological alterations and were more effective than the free drug in inhibiting the growth of cancer cells. In contrast, normal cells seem to be less affected by these formulations. Moreover, F127/VPA microemulsions significantly increased caspase-3 activity in Hela cells (P < 0.05). In vivo administration of free VPA at the dose of 20 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. In addition, the 20 mg/kg of free VPA significantly increased liver lipid peroxidation. The group treated with the 20 mg/kg dose of F127/VPA microemulsions showed increased toxicity compared to the group treated with lignin/VPA microemulsions and free VPA. Molecular simulations revealed that the formation of seven and six-member rings via HB interactions between VPA and F127 influence the stability of the corresponding microemulsion systems and may help to a better action of F127/VPA in comparison to free VPA. Together, our experimental and theoretical findings highlight the opportunity to exploit surfactant microemulsion systems for efficient VPA delivery to target progressive cancer cells and prevent undesirable toxicity leading to tissue injuries.

Antioxidant activities of ethanolic extract of Annona muricata against different pro-oxidant induced lipid peroxidation in rat brain and liver

Antioxidant activities of ethanolic extract of Annona muricata against different pro-oxidant induced lipid peroxidation in rat brain and liver

A fish oil-rich diet leads to lower adiposity and serum triglycerides but increases liver lipid peroxidation in fructose-fed rats

BackgroundConsumption of refined carbohydrates has risen in recent years alongside chronic diseases such as type 2 diabetes mellitus, dyslipidemia, obesity, and non-alcoholic fatty liver disease (NAFLD). Fructose is a monosaccharide made widely available in industrialized products, capable of inducing excessive weight gain and liver steatosis in animal models, while omega-3 fatty acids, present in foods such as fatty fish and fish oil, have shown to inhibit genes related to lipogenesis and decrease cardiovascular risk. Therefore, our objective was to evaluate the impact of a high-fructose diet on weight gain, biochemical and oxidative stress parameters, and liver histology and investigate fish oil’s potential protective role. Thirty male Wistar rats were divided into 3 groups: regular chow diet (CT), regular chow diet plus 20% fructose in drinking water (Fr), and a diet containing 10% fish oil plus 20% fructose in drinking water (FOFr). After 12 weeks, tissues of interest were collected for biochemical and histological analyses.ResultsAlthough fructose consumption did not lead to increased hepatic fat, it caused a significant increase in weight gain, white adipose tissue, and serum triglycerides in the Fr group, while fish oil promoted normalized serum triglycerides and even reduced adiposity in the FOFr group. Additionally, the inclusion of fish oil in the FOFr diet led to increased liver lipid peroxidation in the form of increased hepatic MDA.ConclusionsIt is concluded that fish oil can prevent important metabolic alterations caused by fructose consumption, but its dosage must be taken into account to prevent oxidative stress and potential liver damage.

In vitro and in vivo effect of 3-Para-fluorobenzoyl-propionic acid on rat liver mitochondrial permeability transition pore opening and lipid peroxidation

Abstract The opening of mitochondrial permeability transition (mPT) pore is a well recognized important event in the execution of mitochondrial-mediated apoptosis. Some bioactive compounds induce apoptosis in tumour cells via the induction of mPT pore opening. This study therefore investigated the effect of 3-Para-fluorobenzoyl-propionic acid (3PFBPA), a metabolite of haloperidol on mPT pore, mitochondrial ATPase activity (mATPase), mitochondrial lipid peroxidation (mLPO) and cytochrome c release (CCR). Thirty-two male Wistar rats, were acclimatized for 14 days in clean cages. After 30 days of treatment, they were sacrificed and the liver mitochondria isolated using differential centrifugation. The mPT pore, mATPase, mLPO and CCR were determined by standard methods using a spectrophotometer. The mPT pore opening was induced by 3PFBPA by 1.4, 3.6, 5.6, 6.6 and 7.4 folds, when compared with the control. Also, there was release of cytochrome c and enhancement of mATPase activity by 3PFBPA. The results also show that 3PFBPA reduced lipid peroxidation. However, oral administration of 3PFBPA at 50, 100 and 200 mg/kg did not have any effect on mPT pore opening and mATPase activity when compared with the control but there was inhibition of mLPO. These findings suggested the pharmacological potential of 3PFBPA against the pathological processes related to insufficient apoptosis (based on the in vitro data) and oxidative stress due to its anti-lipidperoxidative effect.

The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study

Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ? 0.73 U/mg) and CAT (147.25 ? 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ? 0.11 U/mg; CAT: 99.37 ? 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ? 0.52 U/mg; CAT: 104.18 ? 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ? 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ? 0.08 nmoL/mg; the fish-based diet group: 1.13 ? 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one?s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants.

Evaluation of days-dependent chloramphenicol dosage on rat liver microsomal lipid peroxidation and catalase activity

Drugs and chemical agents can alter the cellular functions associated with the oxidative metabolism, thereby stimulating ROS production. The objective of the this study was the in vivo study of the inhibitory effect of chloramphenicol on hepatic microsomal enzyme system and the effect on lipid peroxidation using the thiobarbituric acid (TBA) reactive as index of peroxidation damage was investigated. The rats were randomly divided into 3 groups; group 1 serves as the control receiving saline water, group 2 receive chloramphenicol at a dose of 28.6 mg/kg body weight/day for 5 days and group 3 for 7 days. Liver protein content, lipid hydroperoxides and catalase activity were all determined. TBARS concentration increased statistically significantly with time of exposure. After 5 days of treatment, the level of TBARS increased by 103.56% with exogenous oxidant and by 141.54% for without oxidant, as compared to the respective control. On day 7, TBARS level in the liver was approximately 90.75% higher than in the control group with oxidant and 117.03% higher than the control without oxidant. The antibiotic elicited significant increase in rat liver lipid peroxidation compared to control. There were decreases in microsomal protein content of the liver in the drug-treated rats when compared with the control rats. It also showed that chloramphenicol treatment affects cytosolic catalase activities. It decreases catalase levels by 15.75% in 5 days dosage treatment and 17.81% in 7 days dosage treatment respectively. There were significance difference (P&lt;0.05) in the treated and control irrespective of the days of dosage. The catalase level in hepatocyte in 5 days and 7 days group was significance at p&lt;0.01. In conclusion, it was evident that treatment of rats for 5 days and 7 days with the therapeutic doses of Chloramphenicol altered antioxidant system and the intensity of effects was concentration/dosage days dependent, it resulted in membrane lipid peroxidation, protein damage, and inhibition of microsomal catalase due to increased generation of free radicals, reactive oxygen species and reactive nitrogen speciesKeywords: Chloramphenicol, rat liver, lipid peroxidation, catalase activity, antioxidant

Combined effects of celecoxib and cepharanthine on human colorectal cancer cells in vitro

Antioxidant enzyme activity and lipid peroxidation in rat liver exposed to celecoxib and lansoprazole under epinephrine-induced stressBohdan Melekh, Iryna Ilkiv, Andrii Lozynskyi, Alexander Sklyarov

Phytochemical characterization and antioxidant profile of Sechium edule (Jacq) Swartz (Cucurbitaceae) varieties grown in Costa Rica

Context: The Sechium edule fruit is grown and consumed because of their nutritional values in different countries of Latin America. Costa Rica is one of the most important producers and exporters. It is consumed as an antioxidant because its flavonoid content suggests such activity; however, the selection of varieties to crop does not involve the phytochemical profile of them. Aims: To characterize the phytochemical and antioxidant profile of the S. edule varieties grown in Costa Rica. Methods: The in vitro radical scavenging activity of different S. edule extracts were measured using DPPH and ORAC assays, as well as their inhibition of lipid peroxidation in rat liver and their redox potential by cyclic voltammetry. Flavonoids glycosides were isolated by HPLC and their structures were determined by NMR. Results: The S. edule varieties grown in Costa Rica showed a good radical scavenging activity and inhibition of lipid peroxidation; there were significant differences between varieties. In addition, the redox potential determined by electrochemical methods suggested the presence of flavonoids, which was confirmed by the isolation of apigenin 7-O-rutinoside and luteolin 7-O-rutinoside. Conclusions: The antioxidant potential of varieties of S. edule grown in Costa Rica was confirmed, identifying the 845 variety as the best antioxidant profile.

Energy Drink Induced Lipid Peroxidation and Oxidative Damage in Rat Liver and Brain When Used Alone or Combined with Alcohol.

Energy drinks (ED) are containing large doses of metabolic stimulants and its use with ethanol has increased dramatically among young adults. In this study, we examined the effects of ED exposure either alone or in combination with ethanol on oxidative stress parameters including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and lipid peroxidation parameter malondialdehyde (MDA) in rat. Some histopathological findings were also evaluated. ED exposure led to a dose-dependent increase in liver MDA compared to the control indicating oxidative damage. Histopathological findings also revealed that ED alone may generate liver damage. Ethanol exposure increased MDA level and SOD, CAT, and GSH-Px activity in both the brain and the liver. The combination of ethanol and ED produced greater damage which is considered by further increases in SOD and GSH-Px activity in the brain. Similar results for MDA were observed in both the liver and brain as well. Our findings suggest that ED consumption alone or combination with ethanol may represent a significant public health concern.

Antioxidant enzyme activity and lipid peroxidation in rat liver exposed to celecoxib and lansoprazole under epinephrine-induced stress

Antioxidant enzyme activity and lipid peroxidation in rat liver exposed to celecoxib and lansoprazole under epinephrine-induced stress

Assessment of the in vitro anti-lipid peroxidative activity of Costus afer stem extract

Objective: Costus afer is a perennial monocot consumed as a remedy for various ailments. The present study investigates the effect of Costus afer stem extract on ferrous/ascorbate-induced lipid peroxidation in rat brain, liver and kidney homogenates. The inherent antioxidant and free radical scavenging potentials of the extract were also assessed using various in-vitro models. Methods: Homogenates of isolated tissues were prepared and lipid peroxidation was induced using the ferrous/ascorbate system and subsequently incubated with various concentrations of the extract. The ability of the methanolic extract to scavenge hydroxyl radicals, hydrogen peroxide and its ability to reduce ferric ions were investigated. The total antioxidant potential via the phosphomolybdate method was also measured. Results: The results revealed that Costus afer stem extract reduced tissue lipid peroxidation induced by ferrous/ascorbate. The extract also possessed significant antioxidant/free radical scavenging activity via the other in vitro models. In all cases, the effects were concentration-dependent. Conclusion: The methanolic extract of Costus afer stem possessed significant antioxidant potential ascribable to important phytochemicals and could protect cells/tissues from reactive oxygen species-induced damage. Thus the plant has high pharmacological and ethnobotanical prospects.

Inhibition of rat liver mitochondrial membrane permeability transition and lipid peroxidation by seeds extracts of Myristica fragrans Houtt.

Inhibition of rat liver mitochondrial membrane permeability transition and lipid peroxidation by seeds extracts of <i>Myristica fragrans</i> Houtt.

Hematological, biochemical, and toxicopathic effects of subchronic acetamiprid toxicity in Wistar rats.

Acetamiprid is one of the most widely used neonicotinoids. This study investigates toxic effects of repeated oral administration of three doses of acetamiprid (1/20, 1/10, and 1/5 of LD50) during 60days. For this, male Wistar rats were divided into four different groups. Hematological, biochemical, and toxicopathic effects of acetamiprid were evaluated. According to the results, a significant decrease in the body weight gain at the highest dose 1/5 of LD50 of acetamiprid was noticed. An increase in the relative liver weight was also observed at this dose level. The hematological constituents were affected. A significant decrease in RBC, HGB, and HCT in rats treated with higher doses of acetamiprid (1/10 and 1/5 of LD50) was noted. However, a significant increase in WBC and PLT were observed at the same doses. Furthermore, acetamiprid induced liver toxicity measured by the increased activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphates (ALPs), and lactate dehydrogenase (LDH) which may be due to the loss of hepatic membrane architecture and hepatocellular damage. In addition, exposure to acetamiprid resulted in a significant decrease in the levels of superoxide dismutase and catalase activities (p≤0.01) with concomitant increase in lipid peroxidation in rat liver. These findings highlight the subchronic hepatotoxicity of acetamiprid.

In vivo evaluation of graded fresh leave extracts of vernoniaamygdalina and ocimumgratissimum on some oxidative stress indicators in rats

Background: Vernoniaamygdalina and Ocimumgratissimumare widely used plants in the tropics. The leaves of these plants are popularly acclaimed to have medicinal values, with scarce information on the effect of graded extracts in mediating oxidative stress. The interest on these leaves derives from the need to have an insight to the possible effect of thesegraded leaves extracts may have on oxidative stress. Objectives: The objective of the study was to examine the effect of graded extracts of Vernoniaamygdalina and Ocimumgratissimum leaves specifically on free radical inhibition, reducing power and lipid peroxidation in rat liver and kidneys. Methods: The leave samples were collected and air dried, homogenized and sieved to obtain particle sizes of ≤ 0.250mm. Aqueous and methanolic extracts of samples were obtained and used to assay for total alkaloid and phenolic contents, free radical scavenging activity, reducing power and the extent of lipid peroxidationin liver and kidneys were determined, using standard analytical assays. Results: Phenolic contents of both leave extracts were higher in aqueous medium vernoniaamygdalina (0.85 ± 0.07mgGAE/g) and Ocimumgratissimum (1.59 ± 0.13mgGAE/g). The alkaloid contents of the leaves were higher in methanolic extracts, with Ocimumgratissimum highest (0.96 ± 0.04μg/g) and vernoniaamygdalina (0.38 ± 0.01μg/g). The reducing power of these leaves increased with increasing extract concentrations. Ocimumgratissimum had the highest reducing power in both methanolic (3.00 O.D) and aqueous (2.82 O.D) medium in comparison with respective butylatedhydroxytoluene (BHT) (2.49 O.D) and ascorbic acid (2.16 O.D) controls. The ability to scavenge free radicals increased with increasing extract concentrations in both leaves with highest percentages in aqueous extracts. Ocimumgratissimum(95.90%) and vernoniaamygdalina(98.80%) compared to control (100%).The leave extracts of both plants significantly (p<0.05) increased lipid peroxidations in liver and kidneys of rats. Conclusion: Fresh leaves of Ocimumgratissimumand vernoniaamygdalina have shown potentials to be valuable in reducing powers and free radical quenching.

Inhibitory Effect of Phragmanthera Incana (Schum.) Harvested from Cocoa (Theobroma Cacao) and Kolanut (Cola Nitida) Trees on Fe2+ induced Lipid Oxidative Stress in Some Rat Tissues - In Vitro

Evidence in both experimental and clinical studies has shown the participation of oxidative stress in the development and progression of diabetes mellitus. This study therefore, sought to investigate the inhibitory effect of methanolic extract of Phragmanthera incana leaves, a mistletoe species harvested from Cocoa (Theobroma cacao) and Kolanut (Cola nitida) on FeSO4 induced lipid peroxidation in rat pancreas, liver, kidney, heart and brain in vitro. The methanolic extract was prepared with 90% methanol (v/v); subsequently, the antioxidant properties and inhibitory effect of the extract on Fe2+ induced lipid peroxidation in some rat tissues were determined in vitro. Incubation of the different rat tissues homogenate in the presence of Fe caused a significant increase in the malondialdehyde (MDA) contents of the tissues. However, the methanolic extracts of Phragmanthera incana leaves harvested from both Cocoa and Kolanut trees caused a significant decrease in the MDA contents of all the tissues tested in a dose-dependent manner. However, the extract of Phragmanthera incana leaves harvested from kolanut trees had a better inhibitory effect on Fe2+- induced lipid peroxidation in the rat tissues homogenates than that of Phragmanthera incana leaves harvested from cocoa trees. This higher inhibitory effect could be attributed to its significantly higher antioxidant properties as typified by their phenolic content, DPPH radical scavenging ability and reducing power. Therefore, oxidative stress associated with diabetes and its other complications could be potentially managed/prevented by harnessing Phragmanthera incana leaves as cheap nutraceuticals. However, Phragmanthera incana leaves harvested from kolanut trees exhibited better antioxidant properties.