Bean Lines Research Articles

Variability of Nutritional, Antioxidant, and Textural Traits of a Collection of Snap Beans of Different Colors

A set of 54 snap bean lines grown under organic farming was characterized for different traits: pod color, pod cross-section shape, pod section width (PSW), protein content (PC), and sugar content (S). After cooking, the lines were analyzed for firmness and color parameters (CIE-L*, a*, b*). The snap bean lines were grouped based on pod cross-section shape and pod color into eight groups, and significant differences were observed among lines and groups for all the traits investigated. In particular, the yellow pods were harder than the other snap beans and less sweet due to the negative correlation between firmness and sugar content. Fourteen selected lines with contrasting firmness and belonging to different color groups were investigated for their phenolic composition and antioxidant activity (TEAC) before and after domestic cooking. A general decrease was observed only for vanillic acid, quercetin, and apigenin-8-C-glucoside. Quercetin-3-O-rutinoside and kaempferol-3-O-rutinoside were detected in all samples with the highest values in the SBP042 line in both raw and cooked samples. Antioxidant activity decreased with cooking (average of 39%), but purple line SBP053 showed the lowest and no significant loss (3.1%). The results reported in this study could be useful to design specific varieties for different markets and purposes.

Selection of common bean parents and segregating populations targeting fusarium wilt resistance and grain yield

The present work aimed to select populations for the breeding of common bean targeting fusarium wilt resistance and grain yield. Twelve carioca bean lines, which mainly differ in fusarium wilt resistance and grain yield, were crossed in a 6x6 partial diallel scheme. The parents and their 36 F1’s hybrids were evaluated for fusarium wilt severity caused by FOP isolate UFV 01 (FWS), and grain yield (YIELD). 34 F4’s populations, 12 parents and three other lines were also evaluated for grain yield per plant. The data of F1's parents and hybrids were submitted to diallel analysis. Using the grain yield data per plant, the potential of the 34 F4’s populations was predicted by the Jinks and Pooni method (1976). In the diallel analysis, BRSMG Talismã, CVIII 8511, BRS Pérola, VC 25 and VC 13 stood out in terms of the frequency of favorable alleles for FWS. Except for BRSMG Uai and IAC Formoso, these lines presented the most dominant genes associated in Fusarium wilt resistance. For YIELD, there was a predominance of dominant genes determining higher yield. The 20 F4’s populations with the highest potential included the best 12 of the 20 populations, based on diallel analysis for YIELD. Thus, the 12 populations received the addition of four that were highlighted only by the methodology of Jinks and Pooni (1976), and four based on diallel analysis, which totaled 20 populations. The use of information from more advanced inbreeding generations in complementarity with those of diallel is a promising strategy.

Construction of SNP genetic maps based on targeted next-generation sequencing and QTL mapping of vital agronomic traits in faba bean (Vicia faba L.)

Owing to the limitation of a large genome size (~13 Gb), the genetic and gene mapping studies on faba bean (Vicia faba L.) are lagging far behind those for other legumes. In this study, we selected three purified faba bean lines (Yundou 8137, H0003712, and H000572) as parents and constructed two F2 populations. These two F2 populations, namely 167 F2 plants in Pop1 (Yundou 8137×H0003712) and 204 F2 plants in Pop2 (H000572×Yundou 8137), were genotyped using a targeted next-generation sequencing (TNGS) genotyping platform, and two high-density single nucleotide polymorphisms (SNP) genetic linkage maps of faba bean were constructed. The map constructed from Pop1 contained 5 103 SNPs with a length of 1 333.31 cM and an average marker density of 0.26 cM. The map constructed from Pop2 contained 1 904 SNPs with a greater length of 1 610.61 cM. In these two F2 populations, QTL mapping identified 98 QTLs for 14 agronomic traits related to the flowers, pods, plant types and grains. The two maps were then merged into an integrated genetic linkage map containing 6 895 SNPs, with a length of 3 324.48 cM. These results not only lay the foundation for fine mapping and map-based cloning of related genes, but can also accelerate the molecular marker-assisted breeding of faba bean.

A review of Pseudocercospora griseola in common bean ( Phaseolus vulgaris L.) in Africa

Abstract Angular leaf spot (ALS), caused by the fungus Pseudocercospora griseola , is one of the most economically important diseases that limits the productivity of common beans. P. griseola has great variability which explains the large number of existing races and the complexity of genetic resistance. This review was carried out to discuss and document on the diversity of P. griseola in Africa , and the pathogenic variability of pathotypes against different bean varieties and lines. Diversified pathotypes were identified from different common bean-growing countries. About 12 pathotypes were identified from 45 isolates in Uganda. Similarly, about 44 pathotypes were characterized from 100 isolates in Kenya. Pathotypes 61:63 and 63:63 were the most aggressive identified pathotypes in different African countries. Many reports indicated that Andean isolates infect mostly Andean genotypes, while Mesoamerican isolates infect both Andean and Mesoamerican genotypes. The resistances of major commercial varieties and bean lines are breaking down and were found to be susceptible. Hence, multiple resistance breeding should be done for broken commercial varieties and bean lines using the potential identified gene source of landraces U00292, U0074, U351 and U1–9 from Uganda, genotype KKR IL05/Red 40 from Kenya and variety SER125/SER119 from Ethiopia.

Environmental variability in simultaneous selection of common bean for grain quality and mineral concentration traits

The selection of common bean (Phaseolus vulgaris L.) lines based on grain quality and mineral concentration traits will be more efficient if a minimum number of experiments is established. This study was carried out to determine whether grain quality and mineral concentration traits are significantly affected by the genotype × environment interaction; to estimate heritability and genetic gain in individual and combined experiments; and to select superior common bean lines considering a minimum number of experiments. A total of 17 common bean genotypes were evaluated in four experiments. Grain quality was determined through seven traits, and the concentration of six minerals was analyzed by acid digestion. Statistical analyses were completed using data obtained from both individual (I, II, III and IV) and combined (I and II; I, II and III; and I, II, III and IV) experiments. Except for the potassium concentration, all traits showed a significant genotype × environment interaction effect. Heritability and genetic gain estimates of grain quality and mineral concentration traits varied when the data were obtained from one or more experiments. Genetic gain may be inflated because of the data being based on one or two experiments. Four carioca bean (BRS MG Uai, LP 09-33, LEC 01-16 and Pérola) and four black bean (TB 02-19, CHP 04-239-52, TB 03-11 and IAC Netuno) genotypes were selected for their high grain quality and mineral concentration based on four experiments. Data from at least four experiments should be used to select common bean lines superior in grain quality and mineral concentration traits to increase the efficiency of simultaneous selection.

Multivariate analysis applied to the evaluation of genetic variability for the physiological quality trait of common bean seeds

Abstract: Knowledge of the genetic variability and of the variables for evaluating common bean lines for the physiological seed quality trait is important for the selection of promising common bean genotypes for this trait in breeding programs. Through multivariate analysis, the objective of this study was to evaluate the efficiency of physical and physiological tests for studies of genetic variability in seeds of common bean lines. Twenty lines of common bean were evaluated for thousand-seed weight, first count, germination, tetrazolium, electrical conductivity, accelerated aging, field emergence and seedling performance. The experimental data were subjected to analysis of variance, analysis of genetic parameters and multivariate analysis of principal components. Genetic variability was observed among the common bean lines, and two groups of lines with superior physiological performance according to the germination and seed vigor tests were identified. First germination count, germination, seedling length in the field, accelerated aging and emergence speed index tests are the most promising for characterizing the physiological potential of seeds of common bean lines.

Parâmetros genéticos e seleção de linhagens de feijão-carioca resistentes à murcha-de-fusário

Abstract The objective of this work was to estimate genetic parameters and to select lines of carioca commercial bean group with high yield, great 100-seed weight, good grain appearance, and good resistance to fusarium wilt, disease caused by the fungus Fusarium oxysporum f. sp. phaseoli that inhabits the soil. A total of 114 lines coming from two populations were evaluated together with seven control genotypes in the winter crop season in the years/generations of 2015/F5:7 and 2016/F5:8. There is a genetic variability among the carioca common bean (Phaseolus vulgaris) lines for all the traits evaluated, and the estimates of the genetic parameters show a possible successful selection for reaction to fusarium wilt, yield, grain appearance, and 100-seed weight. Twenty-four lines of carioca common bean that were selected combine high resistance to fusarium wilt, yield, 100-seed weight, and good grain appearance. CNFC 19126, CNFC 19205, and CNFC 19131 show a better performance than the control genotypes with the highest level of resistance, so their evaluation in multiple environments is recommended, aiming at new resistant cultivars to Fusarium oxysporum f. sp. phaseoli.

Genetic variability study in advanced mutant lines of cluster bean [Cyamopsis tetragonoloba (L.) Taub.

Advanced cluster bean mutant lines developed by treating seeds of Pusa Navbahar variety with gamma radiation were evaluated in randomised complete block design with two replications to study the genetic variability. The analysis of variance revealed highly significant differences among the mutant lines of cluster bean for all the characters studied. The GCV and PCV values were high (>20 %) for number of vegetable pods per plant, pod yield per plant, pod yield per plot, pod weight, dry pod yield per plant, seed yield per plant and seed yield per plot indicating the existence of broad genetic base, which would be amenable for further crop improvement. High heritability (>60 %) coupled with high genetic advance over mean (>20 %) were observed for characters viz., plant height, number of vegetable pods per plant, number of pods per cluster, pod yield per plant and seed yield per plant which indicated predominance of additive gene action for these traits and hence direct selection would be more effective in improving these traits.

Environmental variability in indirect selection for grain yield in common bean lines

An approach to the number of experiments that should be used in correlation analyses aimed at increasing efficiency in indirect selection for grain yield is unprecedented for common bean (Phaseolus vulgaris L.). We hypothesize that trait correlation estimates vary in response to the growing environment. This study was undertaken to investigate the correlations between plant architecture and yield traits in common bean lines and to determine the minimum number of experiments required by Pearson’s linear correlation analysis to increase efficiency in indirect selection for grain yield. Seventeen common bean genotypes were evaluated for 17 agronomic traits in four experiments. Pearson’s linear correlation analyses were carried out using data from individual experiments and different combinations of growing seasons and years. Ten out of the 17 agronomic traits showed a significant genotype × environment interaction effect, meaning that common bean genotypes exhibited variation for most of the traits evaluated in different growing seasons and years, which resulted in changes in the correlation estimates between these traits. Pearson’s linear correlation estimates obtained between plant architecture and yield traits varied in significance, magnitude, and sign when data from individual experiments and combinations of growing seasons and years were considered. The number of grains per pod is the most promising agronomic trait used in indirect selection for grain yield in common bean lines. Data from at least three experiments should be used in Pearson’s linear correlation analysis to achieve greater efficiency in indirect selection for grain yield in common bean lines.

Ganho genético para caracteres tecnológicos de novas cultivares desenvolvidas pela rede sul brasileira de feijão

ABSTRACT Estimating the genetic gain obtained for technological quality traits over the years makes it possible to analyze whether the changes made meet consumer preferences, which represents an important innovation for common-bean breeding programs. The objectives of this study were to obtain genetic gain estimates for technological grain quality traits of common bean lines and investigate whether the modifications made were favorable in the context of sustainability and food and nutritional security for the coming generations. Twenty-four experiments were conducted between the years 2010 and 2022 and a total of 75 common bean lines were evaluated. Genetic gains of technological grain quality traits were determined by the Vencovsky’s (1988) method adapted for biennia. There was a significant effect of genotype for most traits evaluated, which shows the existence of genetic variability. Mass of 100 grains and water absorption had a negative genetic gain of -2.91% and -0.55% per year, respectively, indicating a reduction in these traits. Genetic gain was zero for L* value (lightness) and cooking time, denoting a plateau for these traits. Most of the common bean lines exhibit adequate L* values for carioca (L* ≥ 53) and black (L* ≤ 22) bean grains; a mass of 100 grains from 20 to 25 g; and fast cooking (≤ 25 min). The new common bean cultivars released for cultivation in the southern region of Brazil have high technological grain quality, being favorable in a context of sustainability and food and nutritional security for the coming generations.

Estratégias de seleção de linhagens de feijão-comum quanto à produtividade e à qualidade comercial de grãos

Abstract The objective of this work was to evaluate common bean lines for yield and commercial grain quality in multi-environments, and to maximize the efficiency of selection using complementary methods. Over three crop years, 79 trials using carioca common bean lines were carried out in the dry, winter, and rainy crop seasons, in the Southern, Southeastern, Midwestern, and Northeastern regions of Brazil. The experimental design was a randomized complete block with three replicates and 17 genotypes. The evaluated traits were: grain yield (GY), grain appearance (GA), sieve yield (SY), and 100-seed weight (100SW). Adaptability and stability analyses were carried out using models that consider the genotype effect as fixed or as random, in a mixed-model perspective. A significant interaction between genotypes and environments was observed for all traits evaluated. The CNFC 15086 line presented the highest GY and 100SW, besides a high adaptability and stability. The most prominent line for GA was CNFC 15038. The CNFC 15097 line is recommended as a cultivar (BRS FC406) because it shows, simultaneously, favorable performances for GY, GA, SY, and 100SW, as well as for adaptability and stability.

Determination of Chemical Fertilizer and Various Organic Fertilizers on Some Agricultural Characteristics of Green Bean (Phaseolus vulgaris L.)

This research was conducted to determine the effects of chemical fertilizers, some organic fertilizers, and control applications on seed yield and some agricultural characteristics of fresh beans in 2021. The trial was carried out in the Faculty of Agriculture of the University of Selçuk more precisely in the research and application station of Prof. Dr. Abdülkadir AKÇİN. In the study, pure bean lines PV2001, Riberia, PV2002, and PV2003 developed by hybridization were used as materials in the experiment. Then the seed yield, some agricultural characteristics, and the protein rate were determined. In this study, it was determined that the effects of chemical fertilizers and some organic fertilizer applications on all other traits examined were found to be statistically significant. As a result of the research, it was determined that the height of the bean plant ranged between 39.81 cm (control) and 51.19 cm (cow manure), the number of pods per plant changed between 14.36 (control) and 27.92 per plant (chemical fertilizer), the number of seeds per plant was 53.76 (control) and 127.52 (chemical fertilizer), the seed yield changed between 116.81 kg da-1 (control) and 239.48 kg da-1 (fertilizer chemical), the weight of 100 g of the seeds ranged between 36.632 g (control) and 40.91 g (sheep manure) and the protein content varies between 26.48 % (cow manure) and 27.49 % (control). The reactions of the bean lines used in the study to chemical fertilizers and some organic fertilizer applications showed differences. As a result, when the results obtained in this one-year study were reviewed, it was revealed that cow and sheep manure could be used in the organic cultivation of beans.

Genetic Variability Studies in F2 Generations of Determinate High Yield Dry Bean Lines for Seed Yield and Yield Components

Dry bean is the third most important pulse crop which is widely adapted and can improve soil fertility in Türkiye. The genetic variability and association studies help in selection which would increase the yield potential of dry bean. The F2 generation and parents (Kınalı, Alberto, Great, Göynük, Özmen) obtained by diallel hybrid method (5 x 5 and 20 combinations were obtained) were sown in the experimental field of Selçuk University Faculty of Agriculture in 2021.In the research, measurement, counting, weighing and analysis of plant height, pod height, number of seeds per plant, number of seeds per pod, seed yield per plant, hundred-seed weight, protein ratio, protein yield in the plant were made. In the F2 generation, the seed yield values in plant of the hybrids are between 23.63 g/plant (Kınalı x Özmen) and 97.45 g/plant (Alberto x Kinalı). Heterosis values of seed yield in plant range from -50.72% (Kınalı x Özmen) to 93.01% (Kınalı x Göynük), heterobeltiosis values varies from -58.92% (Kınalı x Özmen) to 77.26% (Göynuk x Özmen). The protein ratio of the crosses in the F2 generation ranged from 23.05% (Alberto x G Northern 59) to 29.12% (Özmen x Alberto). Heterosis values of protein ratio are between -18.33% (Alberto x G Northern 59) and 7.23% (Özmen x Alberto), heterobeltiosis values are between -22.27% (Alberto x G Northern 59) and 5.82% (Özmen x Alberto). As a result of this research, a sufficient level of genetic variation was determined in the population, considering the agronomic characteristics examined. Determination of suitable parents and hybrids for green bean breeding in terms of sustainability of calcareous soils, agronomic characteristics and inheritance of these parents and hybrids were determined.

Genetic Variability Studies in F2 Generations of Determinate High Yield Fresh Bean Lines for Seed Yield and Yield Components

Fresh bean is the most important pulse crop which are sold fresh, canned, and frozen. The genetic variability and association studies help in selection which would increase the yield potential of fresh bean. The experiment was conducted using randomized block design in 2021 at Konya with 25 genotypes (5 parents and 20 F2 Population). General and special combination abilities, heterosis and heterobeltiosis values, broad and narrow sense heritability, as well as the relationships between the investigated traits, were determined in the parent and F2 hybrids suitable for the diallel analysis method. It was found that there is an additive gene effect in the inheritance of the seed yield trait, and non-additive gene effect in the inheritance of the protein ratio trait. It was determined that the heritability in the narrow sense was low for the seed yield and protein ratio traits. It was also determined that non-additive gene effects were effective in the inheritance of seed yield and protein ratio traits. In F2 generation, the mean heterosis value determined in terms of seed yield was positive and the mean heterobeltiosis value was negative. As a result of this research, a sufficient level of genetic variation was determined in the population, considering the agronomic characteristics examined. Determination of suitable parents and hybrids for green bean breeding in terms of sustainability of calcareous soils, agronomic characteristics and inheritance of these parents and hybrids were determined.

Yield performance and adaptation of advanced large-seeded common bean (Phaseolus vulgaris L.) genotypes in Ethiopia

To ensure the food demand of the rapidly growing population along with changing climate and limiting resources, development and adoption of high-yielding crop cultivars adapting to the adverse effects of climate is crucial for enhancing food production and productivity. The objective of this study was to evaluate the performance and adaptability of 23 advanced bean lines generated by single-seed descent and two commercial varieties (Batu and SAB-736), using additive main effects and multiplicative interaction (AMMI) and genotype plus G x E interaction (GGE) models. Field experiments were conducted in 2019 wet season (from July to October) at five diverse growing environments of Ethiopia. Wide variations were observed across the genotypes and environments for grain yield and other related traits. Mean performance of genotypes for yield ranged from 1.20 to 1.67 t ha −1 across environments, with a grand mean of 1.50 t ha −1 thereby favors genetic enhancement of the trait through selection. Environmental and interaction impacts were greater for all measured traits including yield. Except for number of pod plant −1 and 100 seed weight where interaction effect was more important, the percent variation due to environment was the highest for all traits expression, from 14.76 to 91.09%, to the total variation. Three advanced genotypes showed better yield than SAB-736 (the recent c. variety) and general adaptation across the environments. But, their consistency should be checked across seasons to suggest them for cultivation on these and other similar environments.

Impact of Web Blight on Photosynthetic Performance of an Elite Common Bean Line in the Western Amazon Region of Colombia

Disease stress caused by plant pathogens impacts the functioning of the photosynthetic apparatus, and the symptoms caused by the degree of severity of the disease can generally be observed in different plant parts. The accurate assessment of plant symptoms can be used as a proxy indicator for managing disease incidence, estimating yield loss, and developing genotypes with disease resistance. The objective of this work was to determine the response of the photosynthetic apparatus to the increased disease severity caused by web blight Thanatephorus cucumeris (Frank) Donk on the common bean (Phaseolus vulgaris L.) leaves under acidic soil and the humid tropical conditions of the Colombian Amazon. Differences in chlorophyll fluorescence parameters, including Fv/Fm, Y(II), Y(NPQ), Y(NO), ETR, qP, and qN in leaves with different levels of severity of web blight in an elite line (BFS 10) of common bean were evaluated under field conditions. A significant effect of web blight on the photosynthetic apparatus was found. A reduction of up to 50% of energy use dedicated to the photosynthetic machinery was observed, even at the severity scale score of 2 (5% surface incidence). The results from this study indicate that the use of fluorescence imaging not only allows for the quantifying of the impact of web blight on photosynthetic performance, but also for detecting the incidence of disease earlier, before severe symptoms occur on the leaves.

Combining ability of common bean (Phaseolus vulgaris) genotypes for root traits across diverse environments

AbstractRoot system architecture is important for common bean (Phaseolus vulgaris) adaptability to diverse environments. Beans employ complex adaptive root mechanisms for coping with multiple stresses in production environments. Understanding genetic control of root traits is central to improvement of common bean for adaptation to marginal environments. The objectives of this study were to (i) determine combining ability of root and agronomic traits and (ii) estimate the heritability and genetic correlation of root and agronomic traits in common bean. Four bean lines with superior root traits were crossed with four locally adapted varieties in a North Carolina II mating scheme to generate 16 crosses. The 16 F1s were selfed and advanced to F2 generation. Eight parents and their F2 progenies were evaluated in an alpha‐Lattice design with two replications. General and specific combing ability mean squares were significant (p ≤ .05) for all traits measured. General predictability ratios ranged from .47 to .68 across locations suggesting that both additive and non‐additive gene action modulate root traits and seed yield. Positive and significant (p ≤ .05) phenotypic and genetic correlations revealed significant association between root traits and yield. Moderate to high heritability estimates of between .43 and .67 were realized. Such estimates point to possible deployment of a successful selection programme. Genotype AFR398 displayed significant positive GCA effects among its crosses for both root and agronomic traits hence a potential candidate genotype for inclusion in a bean genetic improvement programme for marginal environments.

Genetic parameters and selection of common bean lines with high nutritional and agronomic quality

AbstractThe aim of common bean (Phaseolus vulgaris) breeding has always been to increase agronomic quality. Recently, the nutritional quality of the grain has been gaining importance. The aim of this study was to estimate genetic parameters for traits related to nutritional and agronomic quality and to select lines for an assessment stage in multiple environments and/or for use as new parents. Initially, 37 lines were evaluated in three environments for nine agronomic and nutritional traits. Of these lines, 15 stood out and were assessed in another five environments. The mixed model (restricted maximum likelihood–best linear unbiased prediction) procedure and multivariate analysis were adopted for processing the phenotypic data. There was genetic variability among the lines assessed for all the traits. The heritability estimates for the agronomic traits ranged from 29% for plant architecture to 94% for reaction to anthracnose. Heritability estimates for the nutritional traits were 77% for grain iron concentration and 79% for zinc concentration. Multivariate analysis, based on all the traits and on all the environments (eight), identified three groups of lines and allowed selection of the lines CNFC 18092, CNFC 18097, CNFC 18114, and BRS Sublime to carry out new crosses, for they showed specific superiority for some traits and the possibility of greater genetic diversity in the populations. The lines CNFC 18091, CNFC 18092, and CNFC 18106 were selected for assessment in experiments in multiple environments because they combine high agronomic and nutritional quality.

Genetic Divergence and Selection of Genitors Aiming to Generate Variability in Common Bean Lines

The objective of this work was to evaluate the genetic divergence between common bean strains from the breeding program of the Agricultural Research Institute of Mozambique (IIAM)-Zonal Centre (CZC) and to identify potential genitors to generate segregating populations with high average and wide genetic variability. Five common bean lines were evaluated in a randomized block design with three repetitions. The quantitative traits evaluated were leaf area index, days to 50% flowering, days to maturity, several pods per plant, 100 seed weight, and yield, while the qualitative traits were growth habit, epicotyl colour, leaf colour intensity, leaf roughness, fresh pod colour, grain colour, grain size, and flower colour. The data obtained were submitted to variance analysis and grouping of means by the Scott-Knott test. The relative importance of characters was evaluated by the Singh. Clustering was performed by the Tocher optimization method and distance projection in the 2D plane, using the generalized Mahalanobis distance as a measure of dissimilarity. The degree of preservation of genetic distances in the 2D plane was assessed by the Cohenetic Correlation Coefficient, distortion, and stress. The LPA54 and Bonus lines were highlighted by direct selection based on the performance per se of their characteristics. Genetic variability was checked for the trait’s days to 50% flowering several of pods per plant, and 100-seed weight and the strains were separated into two groups. The crosses between (G19833, LPA54, Manteiga, and Bonus) with the strain DOR364 there is the potential for obtaining hybrids with higher heterotic effect and segregating populations with high variability. The traits of 100-seed weight, pods per plant, epicotyl colour, and grain size were the most important in the discrimination of the strains

Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.

Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observed on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.