Statistical regression models have been applied in various biotechnical applications to determine the relationship between response and conditional factors as well as to facilitate prediction. This research focuses on sustainable biodegradation process of crude oil using native and recombinant microbial strains to alleviate the significant environmental threat posed by crude oil contamination. This sustainable process is energy and cost-efficient compared to the traditional measures adopted in the extermination of the contaminated soil due to crude oil. The reaction of the wild and recombinant microbial strains to biodegradation rate was detected in the experimental study. These experimental data were used to develop multiple linear regression (MLR) and polynomial regression models (PR). The coefficient of determination, or R2 value, was used to validate the constructed models. The PR model had a high R2 of 0.863, whereas the MLR model had a low value of 0.495. The PR model provided the best match, and it also explains the impact of conditioning factors on the biodegradation rate. To summarize, the recombinant microbial strain yielded a better biodegradation rate with increasing incubation days at lower crude oil contents.
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