Levels Of Non-starch Polysaccharides Research Articles

Dietary starch, non-starch polysaccharides and their interactions affect nutrient digestibility, faecal waste production and characteristics differentially in three salmonids: Rainbow trout, Atlantic salmon and Arctic charr

Waste management has emerged as a critical issue in aquaculture. In this study, we examined the impact of dietary starch and non-starch polysaccharides (NSP) content on nutrient digestibility, faecal waste production, faecal removal efficiency and the faecal characteristics in three salmonid species, namely rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar) and Arctic charr (Salvelinus alpinus). Four diets were formulated according to a 2 × 2 factorial design. The first factor, starch, was tested by including 0% gelatinised wheat flour (low starch) or 20% gelatinised wheat flour (high starch) in a plant-based basal diet. The second factor, NSP, was tested by adding 0% NSP source (low NSP) or 10% NSP source (high NSP). High NSP level was achieved by adding an equal mixture of soya hull (5%) and wheat bran (5%). Diets were tested in triplicates for each species and feeding was done restrictively. Experimental duration was 42 days for rainbow trout and Atlantic salmon and 49 days for Arctic charr. Among the three species investigated, Arctic charr had the lowest digestibility values for most nutrients, whereas rainbow trout and Atlantic salmon values were comparable. High starch level in the diet reduced the macronutrient (protein, fat and ash) digestibility in all three species. High starch and high NSP levels in the diet increased faecal waste production, with the effect being more pronounced for the NSP content of the diet. High dietary starch levels increased the proportion of smaller-sized particles, while high NSP content increased the ability of faecal particles to withstand mechanical stress. The high starch level in the diet lowered faecal removal efficiency but increased by high NSP content. The highest and lowest faecal removal efficiency was recorded for Atlantic salmon and Arctic charr, respectively. The amount of non-removed faeces accumulating in the system was increased by the high starch levels in rainbow trout and Atlantic salmon but remained unaffected by the NSP content of the diet across the species. No interaction effect between starch and NSP content of diets was observed for faecal removal efficiency and non-removed faeces. Among the three species investigated, Arctic charr had the maximum amount of non-removed faeces per kilogram of dry matter feed.

Investigation of metabolic processes of alcohol yeast during the fermentation of buckwheat-corn wort

To expand the range of competitive alcoholic beverages with original organoleptic properties, it is promising to study the use of new types of plant raw materials in grain distillate technologies. The purpose of this work was to establish the influence of the characteristics of the composition of grain wort prepared with the joint use of an alternative raw material source — buck- wheat and corn, which is widely used in alcohol production, on the metabolic processes of the yeast Saccharomyces cerevisiae 985-T and alcoholic fermentation. The objects of the study were buckwheat and corn; enzyme preparations — sources of amylases, xylanases, β-glucanases and proteases; samples of grain wort, mash and mash distillates. The prospects of introducing mixed grain media prepared using non-traditional buckwheat raw materials into the technology of original alcoholic bever- ages are substantiated. It was shown that the use of buckwheat as a source of biocomplete protein, essential amino acids and minerals together with corn containing a high level of starch and a low level of non-starch polysaccharides contributed both to an increase in the yield of alcohol and a change in the quantitative content of secondary metabolites in the composition of volatile impurities. It was found that an increase in the concentration of soluble carbohydrates in the wort and a decrease in its viscosity by 15.5–40.3% occurred due to the inclusion of corn in the composition of the grain mixture, and the enrichment of the wort with nitrogen assimilated by yeast by 19.0–41.8% — as a result adding buckwheat. The optimal ratio of corn and buckwheat in the composition of the grain mixture (7:3) was selected, which made it possible to improve the biochemical and technological parameters of grain must, increase the efficiency of the vital processes of yeast and alcoholic fermentation, ensure an increase in the yield of alcohol by 1.8%, reduce the formation of side metabolites, especially higher alcohols, and change their composition in the direction of increasing the proportion of aldehydes and esters, which can have a positive effect on the organoleptic characteristics of the distillate.

Effects of substituting wheat by rye in diets for young fattening pigs on nutrient digestibility, performance, products of intestinal fermentation, and fecal characteristics.

Climate change and increasing demands to reduce the environmental impact of feed production are major challenges for animal nutritionists. Compared to wheat, which is commonly used in high levels in European piglet diets, rye is more efficient in using limited resources, most importantly, water and phosphorus. As a result, its cultivation has a relatively low carbon footprint. The high amounts of non-starch polysaccharides of rye might lead to an increased intestinal fermentation with potential beneficial effects on gut health. However, the high levels of non-starch polysaccharides in rye, which have a major impact on the physico-chemical conditions of the digesta, might affect digestibility and performance especially in young animals. It was therefore of interest to compare the effects of isoenergetic diets with increasing levels of rye as a replacement for wheat fed to young fattening pigs (bodyweight: 16-40 kg). The control diet contained 69% of wheat, while in the other three experimental diets, the amount of wheat was gradually replaced (by a third in each case) with rye. Thus, the experimental diets contained 23, 46, and 69% of rye. A total of 40 young pigs were housed individually in four dietary treatment groups. During a 4 week trial, effects on performance, digestibility, products of intestinal fermentation, and fecal characteristics were evaluated. There were no negative effects on feed intake and gains, even though the feed conversion ratio increased with the highest dietary rye level (69%). Digestibility rates of organic matter and crude protein did not differ significantly. Without affecting the characteristics of the feces, numerically higher amounts of intestinal fermentation products and higher colonic digesta mass were observed.

SUPPLEMENTATION OF NSPASE IN FULL-FAT SOYBEAN-BASED DIETS IN BROILER CHICKEN

The present study aimed to assess the impact of incorporating commercially available non-starch polysaccharide enzyme (NSPase) in soybean meal (SBM) and full-fat soybean-based diets on broiler bird development, nutritional digestibility, and gut health. Feed costs account for a significant portion, up to 70%, of overall production expenses in poultry businesses. Broiler diets predominantly consist of cereal grains, which contain varying levels of non-starch polysaccharides (NSPs), a fibrous component. The presence of NSPs in broiler diets hampers overall performance by reducing nutrient absorption due to increased digesta viscosity, thereby decreasing feed efficiency. It is estimated that 400-450 kcal/kg of feed remains unmetabolized in broilers fed a standard corn-soy diet. Exogenous enzymes function by making previously indigestible substances accessible for digestion by endogenous enzymes. NSPase enzyme has the potential to enhance digestion by reducing viscosity and releasing energy through the breakdown of undigested feed components.

High dietary non-starch polysaccharides detrimental to nutrient digestibility, digestive enzyme activity, growth performance, and intestinal morphology in largemouth bass, Micropterus salmoides.

An 8-weeks feeding trial was carried out to evaluate the effects of different levels of dietary non-starch polysaccharide on the growth, apparent nutrient digestibility, intestinal development, and morphology of largemouth bass (Micropterus salmoides). Seven isoproteic and isolipidic experimental diets were formulated (crude protein 47.00%, crude lipid 12.50%), containing 0, 3, 6, 9, 12, 15, and 18% non-starch polysaccharides (NSPs) (named Control, NSPs3, NSPs6, NSPs9, NSPs12, NSPs15, and NSPs18), respectively. Dietary inclusion of NSPs below 9% showed no negative impacts on fish growth and feed utilization efficiency, whereas dietary NSPs inclusion level above 9% decreased weight gain rate, specific growth rate, protein efficiency, protein deposition rate, apparent digestibility of dry matter and protein, and were accompanied by a reduction in intestinal protease, Na+/K+-ATPase and alkaline phosphatase activity and an increase in feed intake and feed coefficient. The activity of lipase was significantly decreased when dietary inclusion of 15 and 18% NSPs. Moreover, the lipid deposition rate and the apparent digestibility of lipids were significantly decreased since dietary inclusion of 9% NSPs. Dietary inclusion of NSPs above 12% significantly up-regulated intestinal GLP-2 gene's expression, and was accompanied by significant changes in hindgut morphology, including increases in villus length and width, muscularis thickness and number of goblet cell, as well as a decrease in crypt depth. Additionally, dietary inclusion of NSPs above 3% significantly increased intestinal length index, and the viserosomatic index was significantly increased when dietary NSPs exceeded 15%. The linear regression analysis based on weight gain rate and feed coefficient showed that the appropriate dietary NSPs level of juvenile largemouth bass should not above 5.51%. In conclusion, high dietary NSPs adversely affects digestive enzyme activity and intestinal morphology, which in turn reduced the apparent digestibility of dietary nutrients and growth of juvenile largemouth bass.

In vitro evaluation of efficacy of nonstarch polysaccharides enzymes on wheat by simulating the avian digestive tract.

In this study, the efficacy of different nonstarch polysaccharide (NSP) enzyme sources on wheat ingredients and wheat basal diets in vitro were evaluated by simulating the avian digestive tract. In Exp. 1, pH level was increased from 2.0 to 8.0 by simulating the avian digestive tract. The relative enzyme activities of xylanase A, B, and C and β-glucanase X at pH 3.0-3.5 were higher (P < 0.05) than those at pH 2.0 or 7.0-8.0. The optimal pH levels of 3.5 and 7.0 were screened by simulating the proventriculus and small intestine, respectively to evaluate the efficacy of NSP enzyme on wheat sources. In Exp. 2, wheat 1 contained the highest content of NSP fractions and the lowest digestibility in vitro dry matter (IVDMD) and energy (IVED) in wheat samples. Therefore, wheat 1 was selected for hydrolysis research under different NSP enzyme sources and levels (1,500, 4,500, 13,500, 40,500, 121,500 U xylanase/kg and 250, 500, 1,000, 2,000, 4,000 U β-glucanase/kg) in vitro. The hydrolysis of wheat on the basis of the released reducing sugar content was determined by xylanase sources A > B > C (P < 0.05) and β-glucanase sources of X > Y (P < 0.05). On the basis of the hydrolysis, the optimum dose of xylanase A and β-glucanase X were 40,500 U/kg and 2,000 U/kg, respectively. Subsequently, the completely randomized designs involving 2 NSP enzymes treatments × 2 endogenous digestive enzymes treatments (Exp. 3), as well as 2 wheat basal diets × 2 NSP enzymes treatments (Exp. 4) were used to evaluate the efficacy of NSP enzymes on dietary nutrient digestibility. The addition of NSP enzymes (40,500 U xylanase A/kg and 2,000 U β-glucanase X/kg) increased the IVDMD and IVED of wheat 1 without endogenous enzymes (P < 0.05), while the IVDMD and IVED of wheat 1 with endogenous enzyme were only slightly increased (P > 0.05). The addition of NSP enzymes could increase the IVDMD and IVED of corn-wheat-soybean meal diet (P < 0.05), but had no effect on those of wheat-cottonseed meal rapeseed meal diet (P > 0.05). In conclusion, xylanase and β-glucanase additions could effectively eliminate the adverse effects on wheat and wheat basal diets at the optimal pH levels of 3.5 and 7.0 by simulating the proventriculus and small intestine parts, respectively. The efficacy of NSP enzymes was influenced by the enzyme sources, dietary type, and the interaction of endogenous enzymes.

Loss of starch synthase IIIa changes starch molecular structure and granule morphology in grains of hexaploid bread wheat

Starch synthase III plays a key role in starch biosynthesis and is highly expressed in developing wheat grains. To understand the contribution of SSIII to starch and grain properties, we developed wheat ssIIIa mutants in the elite cultivar Cadenza using in silico TILLING in a mutagenized population. SSIIIa protein was undetectable by immunoblot analysis in triple ssIIIa mutants carrying mutations in each homoeologous copy of ssIIIa (A, B and D). Loss of SSIIIa in triple mutants led to significant changes in starch phenotype including smaller A-type granules and altered granule morphology. Starch chain-length distributions of double and triple mutants indicated greater levels of amylose than sibling controls (33.8% of starch in triple mutants, and 29.3% in double mutants vs. 25.5% in sibling controls) and fewer long amylopectin chains. Wholemeal flour of triple mutants had more resistant starch (6.0% vs. 2.9% in sibling controls) and greater levels of non-starch polysaccharides; the grains appeared shrunken and weighed ~ 11% less than the sibling control which was partially explained by loss in starch content. Interestingly, our study revealed gene dosage effects which could be useful for fine-tuning starch properties in wheat breeding applications while minimizing impact on grain weight and quality.

Effects of dietary non-starch polysaccharides level on the growth, intestinal flora and intestinal health of juvenile largemouth bass Micropterus salmoides

A 56-day feeding trial was conducted to evaluate the effects of dietary non-starch polysaccharide (NSPs) inclusion on the growth performance, intestinal flora and intestinal health of juvenile largemouth bass (6.00 ± 0.01 g) in quadruplicate. Seven isoproteic and isolipidic diets (crude protein 47.0%, crude lipid 12.5%) were formulated to contain 0%, 3%, 6%, 9%, 12%, 15% and 18% NSPs (each 100 g contains 32.26 g cellulose, 19.35 g arabinoxylan, 4.52 g β-glucan, 5.16 g mannan and 38.71 g pectin; named N0, N3, N6, N9, N12, N15 and N18), respectively. Weight gain rate, specific growth rate, protein efficiency ratio, protein and lipid deposition rate in the N12, N15 and N18 groups were significantly lower than in the N0 group. Plasma diamine oxidase activity and lipopolysaccharide and endothelin-1 concentration in the N15 and N18 groups were significantly higher than in the N0 group. Intestinal occludin, caludin-1, caludin-4, zonulaoccludens-1, interleukin-10, transforming growth factor-β, nuclear factor E2-related factor 2, B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-xl and Bcl-2-associated athanogene expression levels and intestinal peroxidase, catalase and superoxide dismutase activities in the N15 and N18 groups were significantly lower than in the N0 group, while interleukin-8, interleukin-1β, tumor necrosis factor-α, kelch-like ECH-associated protein 1, caspase3, Bcl-2-assoxicated x protein and Bcl-2-associated death protein genes expression levels showed opposite trends. Moreover, the Shannon, Simpson, Chao and Ace indices and the abundance of Plesiomonas shigelloides, [Clostridium] dakarense and Romboutsia ilealis in the N18 group were significantly lower than in the N0 group, in contrast to the abundance of Paraclostridium bifermentans, Mycoplasma moatsii. Propionate, butyrate, valerate and total short-chain fatty acids (SCFA) contents in digesta of the N6 group were significantly higher than in the N0 group, while acetate, butyrate, valerate, caproate and total SCFA in digesta of the N18 group were significantly lower than in the N0 group. Results suggest that high NSPs diets increased intestinal permeability and abundance of pathogenic microorganisms, induced intestinal inflammation and intestinal epithelial cell apoptosis, but decreased intestinal flora α-diversity and SCFA contents, and ultimately impaired growth performance and intestinal health of juvenile largemouth bass. Conversely, low-dose NSPs inclusion had no negative effect on growth and improves intestinal health in juvenile largemouth bass, showing potential as prebiotics in aquafeed.

Ileal profile of non-starch polysaccharides and oligosaccharides in response to exogenous enzymes in broiler chickens offered wheat- or maize-based diets under subclinical necrotic enteritis challenge.

The present study evaluated the impacts of fibre-degrading enzymes on the profiles of non-starch polysaccharides (NSP) and oligosaccharides (OS) in the ileum of broiler chickens offered wheat- or maize-based diets under subclinical necrotic enteritis (NE) challenge. A 2 × 2 × 4 factorial arrangement of treatments was used. Factors were the following: NE challenge, no or yes; diet type, wheat- or maize-based; and supplemental enzymes, control (no enzyme), family 10 xylanase (XYN10), family 11 xylanase (XYN11) or β-mannanase (MAN). Birds in the challenged group were inoculated with Eimeria on d 9 and Clostridium perfringens on d 14 and 15. A 3-way interaction (P = 0.047) occurred on overall (d 0 to 16) weight gain. When NE was present, all the supplemental enzymes increased weight gain in birds fed the wheat-based diet; whereas in those fed the maize-based diet supplemental XYN10 and XYN11 decreased weight gain. When NE was absent, birds fed the wheat-based diet supplemented with XYN10 or MAN presented increased weight gain compared to non-supplemented birds, but no improvements with enzyme addition were observed in birds fed the maize-based diet. A 3-way interaction (P = 0.002) was observed on insoluble NSP level in the ileum. When NE was absent, all the supplemental enzymes reduced the ileal level of insoluble NSP, regardless of diet type. In the challenged birds, supplementing XYN10 and MAN reduced insoluble NSP level in the ileum, but only in birds fed the wheat-based diet. Ileal soluble NSP level was reduced by supplemental XYN11 and MAN, but only in birds fed the wheat-based diet, resulting in a 2-way diet type × enzyme interaction (P < 0.001). Ileal OS arabinose (P = 0.030) level was highest in birds offered the wheat-based diet supplemented with XYN11. Collectively, supplementation of NSP-degrading enzymes to the wheat-based diet enhanced bird performance regardless of NE challenge, with XYN11 significantly increasing oligosaccharide release. However, enzyme addition did not improve growth performance in birds fed maize-based diet, with supplemental XYN10 and XYN11 impeding weight gain when NE was present.

In vitro degradation of non-starch polysaccharide residues in the digesta of broilers offered wheat-soy or maize-soy diets by feed enzymes

The present study evaluated the in vitro efficacy of different feed enzymes on degrading non-starch polysaccharide (NSP) residues present in digesta collected from broilers’ gastrointestinal tract. Two dietary treatments, wheat-soy or maize-soy based diets, were assigned to broilers (12 replicates per treatment; 10 birds per replicate pen) from d 0 to 35. At 35 d of age, digesta was collected from the gizzard, jejunum and ileum from three birds per replicate and pooled. The digesta samples were digested in vitro with one of nine enzymes; GH10, GH11 and GH8 xylanase, arabinofuranosidase, a combination of GH10 xylanase and β-glucanase (XYN+BG), β-mannanase, protease, cellulase cocktail and pectate lyase. When using the gizzard digesta as substrate, GH8 xylanase reduced (P&lt;0.005) both insoluble and soluble NSP levels compared to the control, regardless of diet type. Analysis of NSP degradation in jejunal digesta from birds fed the wheat-soy diet revealed that the three xylanases and arabinofuranosidase reduced (P&lt;0.001) insoluble NSP level compared to the control, but the soluble NSP level was not affected by the enzyme treatments. All enzymes, except the cellulase cocktail and pectate lyase, reduced (P=0.005) the insoluble NSP level in the jejunal digesta collected from birds fed the maize-soy diet. Similarly, all enzyme preparations reduced (P&lt;0.001) the insoluble NSP level in ileal digesta obtained from birds fed the maize-soy diet. The GH10 and GH11 xylanase, XYN+BG, cellulase cocktail and pectate lyase reduced (P&lt;0.001) the insoluble NSP level in the ileal digesta from birds fed the wheat-soy diet compared to the control, with XYN+BG exhibiting the greatest reduction. Collectively, the present in vitro study indicated that the in vitro efficacy of enzyme preparations was dictated by the amount and type of NSP remaining undigested in various parts of the gut, which perhaps affect the in vivo efficacy.

The flow of non-starch polysaccharides along the gastrointestinal tract of broiler chickens fed either a wheat- or maize-based diet.

The present study characterised the types and amounts of non-starch polysaccharides (NSP) remaining undigested along the gastrointestinal tract (GIT) of broiler chickens offered a typical wheat- or maize-based diet. One-day old Cobb 500 mixed-sex chicks were assigned to 24 pens, with 10 birds/pen and 12 pens/treatment. Birds were offered the experimental diets in 3 phases (starter, day 0 to 10; grower, day 11 to 24 and finisher, day 25 to 35). Excreta and digesta samples from the crop, gizzard, duodenum, jejunum, ileum and caeca were collected at 12 and 35 days of age, and analysed for the NSP flow. The wheat-based diet contained higher levels of soluble NSP than the maize-based diet, whereas insoluble NSP levels were similar between the 2 diets. Detailed analysis of NSP constituents revealed that arabinoxylans were the primary NSP in the wheat-based diet, mostly in insoluble form. Pectins were the predominant NSP in the maize-based diet, followed by arabinoxylans. Overall, birds offered the wheat-based diet presented higher levels of soluble NSP remaining in all gut sections compared to birds offered the maize-based diet, at both 12 and 35 days of age (P < 0.050). Accumulation of insoluble NSP in the gizzard was noted in birds fed both diets, but was more pronounced in birds offered the maize-based diet compared to the wheat-based diet, at both 12 and 35 days of age (P < 0.001). The present study highlights marked differences in the amounts and types of NSP delivered to the different gut sections when feeding wheat-compared to maize-based diets, particularly in the gizzard and the lower GIT of birds.

Multi-carbohydrase application into energy and amino acid deficient broiler diets: A strategy to enhance performance of broiler chickens.

The effect of Multi-Carbohydrase (MC) supplementation on growth performance, visceral organ weights, blood metabolites, jejunum morphology, nutrient digestibility, and carcass parameters of broiler chickens fed nutrient-deficient corn soybean-meal based diets containing high levels of non-starch polysaccharides from wheat and wheat by-products was investigated. A total of 378 one-day-old Ross 308 broiler chickens were randomly assigned to one of seven dietary treatments to give six replicates per treatment (nine birds per pen). Dietary treatments were as follows: (1) positive control (PC; commercial standard diet); (2) negative control 1 (NC-1; PC-120 kcal/kg metabolizable energy); (3) NC-2 (PC-3% standardized ileal digestibility [SID] amino acids). The remaining four dietary treatments were formulated with the addition of MC (MC; Superzyme-CS™) into two negative controls along with two supplementation levels of MC (i.e., 0.025% and 0.05%, respectively). Improved body weight, average daily gain, and feed conversion ratio (p < 0.05) were observed in broiler chickens fed a reduced energy diet supplemented with MC compared to birds fed NC-1 diet from days 1-35. Additionally, birds fed a reduced energy diet with 0.05% MC showed comparable (p > 0.05) growth performance with birds fed PC for 35-day post-hatch. Furthermore, the addition of MC into reduced amino acid diets improved (p < 0.05) growth performance. Broiler chickens fed MC supplemented nutrient-deficient diets showed a greater (p < 0.05) villus height to crypt depth ratio than birds fed diets without MC on days 21 and 35. Similarly, improved (p < 0.05) nutrient digestibility was observed in birds fed reduced energy diets supplemented with MC compared to birds fed NC-1 on days 21 and 35. Our results suggest that MC supplementation into reduced energy or reduced amino acid diets containing wheat and wheat by-products has the potential to improve growth performance and nutrient digestibility while maintaining healthier gut morphology in broiler chickens from 1 to 35 days of age.

Effect of enzymes (phytase and xylanase), probiotics (B. amyloliquefaciens) and their combination on growth performance and nutrient utilisation in Nile tilapia

The increased use of plant ingredients in aquafeeds over the last decades, as replacement for fish meal, has led to rising levels of undesired non-starch polysaccharides (NSP) and phytate. Both NSP and phytate degrading enzymes and probiotics have been widely studied. They can be used as a tool to deal with increasing levels of NSP and phytate in aquafeeds. However, studies combining both probiotics and enzymes are scarce in fish. The main objective of the present study was to assess the impact on Nile tilapia (Oreochromis niloticus) of enzymes and probiotics, as well as their synergistic effect. Parameters measured were: growth; nutrient digestibility; body composition; and the energy, nitrogen (N), P and Ca balance. Diets were supplemented with, and without, an enzyme mix (phytase at 1000 FTU/kg and xylanase at 6000 U/kg) and with, and without, probiotics (three strains of B. amyloliquefaciens at 60 mg/kg feed), according to a 2 × 2 factorial arrangement. This resulted in a control treatment (CON-CON) without enzymes and probiotics, an enzyme treatment (ENZ-CON), a probiotic treatment (CON-PRO) and a treatment with both enzymes and probiotics (ENZ-PRO). In total, 16 tanks (4 replicates/treatment) were used with 35 fish each (mean initial weight 39 g). Fish were restrictively fed equal amounts of dry matter for 42 days. Both enzymes (P < 0.001) and probiotics (P < 0.05) improved growth (g/d) and FCR when applied individually. The combination of enzymes and probiotics showed an interaction effect (P < 0.05) on growth and FCR. Enzymes improved growth to a greater extent than probiotics, whereas the combination of enzymes and probiotics did not further enhance growth. The CON-CON treatment had the highest FCR (1.33), the CON-PRO treatment a slightly lower FCR (1.27); the lowest FCR (1.11) was found for both treatments with enzymes (ENZ-CON and ENZ-PRO). Enzyme supplementation improved the digestibility of all nutrients (P < 0.01), whereas probiotics enhanced fat digestibility (P < 0.01). Additionally, enzyme supplementation increased retained P (mg/d), retained N (mg/d) and N efficiency (P < 0.001). Probiotic supplementation affected the energy requirements for maintenance (kJ/kg0.8/d; P < 0.05). Dietary supplementation of either enzymes or probiotics had positive effects on the measured parameters, but the combination of enzymes and probiotics did not have a synergistic effect.

Xylanase supplementation in corn-based swine diets: a review with emphasis on potential mechanisms of action.

Corn is a common energy source in pig diets globally; when financially warranted, industrial corn coproducts, such as corn distiller’s dried grains with solubles (DDGS), are also employed. The energy provided by corn stems largely from starch, with some contribution from protein, fat, and non-starch polysaccharides (NSP). When corn DDGS are used in the diet, it will reduce starch within the diet; increase dietary protein, fat, and NSP levels; and alter the source profile of dietary energy. Arabinoxylans (AXs) comprise the majority of NSP in corn and its coproducts. One strategy to mitigate the antinutritive effects of NSP and improve its contribution to energy is by including carbohydrases within the diet. Xylanase is a carbohydrase that targets the β-1,4-glycosidic bonds of AX, releasing a mixture of smaller polysaccharides, oligosaccharides, and pentoses that could potentially be used by the pig. Xylanase is consistently effective in poultry production and moderately consistent in wheat-based swine diets, but its efficacy in corn-based swine diets is quite variable. Xylanase has been shown to improve the digestibility of various components of swine-based diets, but this seldom translates into an improvement in growth performance. Indeed, a review of xylanase literature conducted herein suggests that xylanase improves the digestibility of dietary fiber at least 50% of the time in pigs fed corn-based diets, but only 33% and 26% of the time was there an increase in average daily gain or feed efficiency, respectively. Intriguingly, there has been an abundance of reports proposing xylanase alters intestinal barrier integrity, inflammatory responses, oxidative status, and other health markers in the pig. Notably, xylanase has shown to reduce mortality in both high and low health commercial herds. These inconsistencies in performance metrics, and unexpected health benefits, warrant a greater understanding of the in vivo mechanism(s) of action (MOA) of xylanase. While the MOA of xylanase has been postulated considerably in the literature and widely studied in in vitro settings, in wheat-based diets, and in poultry, there is a dearth of understanding of the in vivo MOA in pigs fed corn-based diets. The purpose of this review is to explore the role of xylanase in corn-based swine diets, discuss responses observed when supplemented in diets containing corn-based fiber, suggest potential MOA of xylanase, and identify critical research gaps.

The resistant starch from sorghum regulates lipid metabolism in menopausal rats via equol.

Equol is a metabolite of daidzein and has a higher biological activity than daidzein. High levels of non-starch polysaccharides can stimulate fermentation in the intestine leading to rapid conversion of daidzein into equol that has great potential to reduce obesity in postmenopausal women. In the present study, female Sprague-Dawley rats were used to establish a menopausal model by oral administration of formestane and to compare the protective effect of resistant starch on lipid metabolism, with or without soybean feed. The resistant starch was found to effectively control body weight and adipose tissue quality, while increasing the high-density lipoprotein cholesterol (HDL-C) concentration and lowering the glycerol, triacylglycerols (TG), total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C) concentrations with soybean feed. Equol inhibited the expression of SREBPC1 gene by inhibiting SHP in the liver via transcription factor FXR, thereby inhibiting the synthesis of triglyceride and fatty acid in the liver. PRACTICAL APPLICATIONS: Intake of a certain amount of resistant starch while eating the soy product can better regulate lipid metabolism in menopausal obese rats compared to consumption of resistant starch alone. Studies have shown that resistant starch converts daidzein to Equol by regulating the structure of the intestinal flora and acts as an estrogen in menopausal rats. This research will further expand the health applications of resistant starch and provide useful information for the food industry.

Fermented wheat bran by xylanase-producing Bacillus cereus boosts the intestinal microflora of broiler chickens

Wheat bran, while a nutritious and economic feed ingredient, contents high levels of non-starch polysaccharides which entraps nutrients and interferes digestion and absorption. To study the influence of fermented wheat bran by xylanase-producing Bacillus cereus on growth performance and intestinal microflora of broiler chickens, a total of 180 broilers (21-day-old, mixed of male and female) were randomly divided into 3 treatments, with 6 replicates in each treatment and 10 broilers in each replicate: 1) control check (CK), corn-soybean meal-based diet; 2) wheat bran group (WB), 5% of the corn were replaced with wheat bran; and 3) fermented wheat bran group (FWB), 5% of the corn were replaced with fermented wheat bran. Growth performance was determined in the period of 21- to 42-day-old. Intestinal digestive enzyme activities and microbiota diversity were analyzed on day 42. No differences were observed on growth performance among treatments (P > 0.05). The activity of amylase in the duodenum of FWB was 1.56 times higher than CK (P < 0.05). The Chao1 index of microbiota in cecum of FWB increased 24.26% compared with CK (P < 0.01). The amount of Bifidobacteriaceae in cecum of WB was 29.1 times and 15.8 times higher than CK and FWB (P < 0.05) respectively. Principal co-ordinates analysis in cecum revealed the dissimilarity microbiota among treatments. In summary, the use of fermented wheat bran to partially replace corn (5%) in diets had no adverse effect on growth performance and triggered beneficial effects such as increasing duodenal amylase activity and intestinal microflora abundance in broiler chickens. These observations support that solid-state fermentation by xylanase-producing Bacillus cereus is feasible approach to pre-treat wheat bran for feedstuff industry.

Viscosity and growth response of broilers fed high fiber diets supplemented with a corn-produced recombinant carbohydrase

SUMMARY Feed ingredients containing high levels of non-starch polysaccharides (NSP), such as wheat and corn distillers’ dried grains with solubles (DDGS), can form viscous digesta in the gastrointestinal tract, limiting broiler performance. A study was conducted to determine efficacy of a corn-produced recombinant carbohydrase (AC1) on dietary and intestinal viscosity and broiler performance with a high NSP diet. Hubbard × Ross 708, a day old, male broiler chicks (n = 960) were fed 1 of 8 dietary treatments for 21 d. Diets included a corn–soybean meal based diet (PC_1) and a diet of similar essential nutrient density, but with a 10% inclusion of both wheat and DDGS (PC_2) and a negative control (NC) with similar ingredients as PC_2, but with ME reduced by 125 kcal/kg. Additional treatments had varying levels of AC1, supplying 50, 100, 200, 400, or 600 U β-Glucanase (β-Glu-U) per kg of feed, mixed into the NC diet. Dietary and digesta (d14) viscosity and weekly bird performance were measured. The inclusion of wheat and DDGS increased d1–7 feed conversion ratio (FCR) and supplementing AC1 at 50–400 β-Glu-U/kg reduced FCR equivalent to PC_1. Intestinal viscosity correlated with d1–21 FCR and inversely correlated with d1–21 live weight gain (LWG). When analyzed categorically, dietary viscosity inversely correlated with d1–21 LWG (P

Effects of Different Papua New Guinea Sweetpotato Varieties on Performance and Level of Enteric Pathogens in Chickens.

Simple SummaryThe smallholder poultry industry in Papua New Guinea (PNG) has grown rapidly in the last decade. The cost of growing meat birds is high, as feed alone makes up to 80% of the total cost of production in PNG. Sweetpotato is currently used in a poultry feed as a cheaper alternative option compared to the more expensive commercially manufactured stockfeed in PNG. The PNG smallholder poultry production system involves many families who rear multiple batches of meat birds every year. These birds are sold mostly at the farm gate, at local provincial markets, or roadside markets. Consumption of contaminated chicken meat has been identified as one of the important food vehicles for food borne illness. This PNG-based study was conducted to understand whether the inclusion of local sweetpotato in poultry feed can influence the shedding of pathogens such as Clostridium perfringens, Salmonella and Campylobacter without causing negative effects on poultry performance. The results of this study releveled that Campylobacter and Salmonella levels in the broilers fed with the local sweetpotato diets can be influenced with inclusion of enzymes in the feed. In the last decade, research has targeted the evaluation of local feed ingredients for use in monogastric diets to alleviate the high cost of production of livestock at smallholder levels in Papua New Guinea (PNG). The PNG smallholder poultry production system involves many families who rear multiple batches of meat birds every year. This study was conducted to evaluate the levels of enteric pathogens in the caeca of broilers fed with sweetpotato diets with varying levels of non-starch polysaccharides (NSP). Selection of a sweetpotato variety for use in broiler diets should be based on the total NSP content. In particular, varieties with low soluble NSPs are economical to use as Apparent Metabolizable Energy (AME) values are within the desired range for poultry and there is minimal need to include enzymes to improve NSP digestibility. The use of varieties with a low total NSP is also advantageous as the numbers of Clostridium perfringens was lower in broilers fed with these sweetpotato varieties. The level of Campylobacter and Salmonella levels were high in the ceca of birds fed with the sweetpotato varieties with high total NSP. These levels can be reduced with the inclusion of enzymes. This information will assist in the efficient use of local varieties of sweetpotato in PNG by small holder poultry farmers for sustainable poultry production and the commercial industry.

Selection of carbohydrate-active probiotics from the gut of carnivorous fish fed plant-based diets

The gastrointestinal microbiota plays a critical role on host health and metabolism. This is particularly important in teleost nutrition, because fish do not possess some of the necessary enzymes to cope with the dietary challenges of aquaculture production. A main difficulty within fish nutrition is its dependence on fish meal, an unsustainable commodity and a source of organic pollutants. The most obvious sustainable alternatives to fish meal are plant feedstuffs, but their nutritive value is limited by the presence of high levels of non-starch polysaccharides (NSP), which are not metabolized by fish. The composition of fish-gut microbial communities have been demonstrated to adapt when the host is fed different ingredients. Thus, we hypothesized that a selective pressure of plant-based diets on fish gut microbiota, could be a beneficial strategy for an enrichment of bacteria with a secretome able to mobilize dietary NSP. By targeting bacterial sporulating isolates with diverse carbohydrase activities from the gut of European sea bass, we have obtained isolates with high probiotic potential. By inferring the adaptive fitness to the fish gut and the amenability to industrial processing, we identified the best two candidates to become industrially valuable probiotics. This potential was confirmed in vivo, since one of the select isolates lead to a better growth and feed utilization efficiency in fish fed probiotic-supplemented plant-based diets, thus contributing for sustainable and more cost-effective aquaculture practices.

PSX-31 Effects of Dietary Non-starch Polysaccharide (NSP) and NSP-Degrading Enzyme Complex (Endo-PowerTM) Supplementation on Growth Performance, Intestinal Environments and Systemic Immune Responses in Growing Pigs.

The objective of this experiment was to investigate the effects of non-starch polysaccharide (NSP) and NSP-degrading enzyme complex (NEC) levels on growth performance and gut environment in growing pigs. NEC is known to improve the utilization of nutrient in feed. Beside of this increased digestibility, NEC is also known to promote gut health by increased villus height. Therefore, we conducted an experiment with diets included NSP-rich feed ingredients (wheat and barley) and NEC (Endo-PowerTM; α-1,6-galactosidase and β-galactomannanase). One hundred eight 8-week-old pigs were randomly allotted into a 2x3 factorial designed experiment with 6 replications. The high- (H-) or low-NSP contained (L-) diet supplemented with 3 dietary levels of NEC, 0% (CON), 0.1% (NEC-0.1), and 0.2% (NEC-0.2), was provided for four weeks. One pig from each pen was sacrificed to collect plasma, jejunum, ileal content and cecal content samples. The data was analyzed using ANOVA procedures of SAS 9.4. The means were compared by Duncan’s multi-range test at P<0.05 level of significance. Body weight, average daily gain, and feed intake in L-NEC-0.1 and H-NEC-0.2 treatments were significantly higher (P<0.05) than other treatments during the whole experimental period. In L-diet treatments, cecal population of coliform was significantly reduced and cecal butyrate concentration was significantly increased by NEC-0.1 supplementation (P<0.05). And in H-diet treatments, plasma IL-4 and IL-6 levels were significantly decreased, and jejunal villus heights and ileal energy digestibility were significantly increased by NEC-0.2 supplementation (P<0.05). These results imply that growth-promoting effects of NEC were maximized in L-NEC-0.1 and H-NEC-0.2 treatments via modulation of gut environments. It seems that NEC’s mechanism in gut appears to be different depending on the dietary NSP levels.