Inorganic Phosphorus Levels Research Articles

Prevalence and severity of sarcopenia in patients on maintenance hemodialysis: a cross-sectional study

BackgroundSarcopenia, the progressive loss of muscle mass and strength, is common among patients with chronic kidney disease, especially those on maintenance hemodialysis. This condition often arises from factors like reduced physical activity and metabolic changes associated with chronic kidney disease. This study aims to assess the prevalence and severity of sarcopenia in patients on maintenance hemodialysis (MHD), focusing on probable sarcopenia and its correlations with physical activity, body mass index, and inorganic phosphorus levels.MethodsThis cross-sectional study involved 220 patients on MHD who visited to West China Hospital of Sichuan University between February and April 2023. The demographic data, body measurements, and laboratory results were retrospectively collected. Sarcopenia was assessed using the 2019 criteria from the Asian Working Group and the European Working Group on Sarcopenia in Older People, and physical activity was measured via the International Physical Activity Questionnaire.ResultsOf the study participants, 141 (64.1%) were diagnosed with probable [110 (50.0%) or confirmed sarcopenia [31 (14.1%)], including 22 (10%) with severe sarcopenia. Multivariable logistic regression analysis revealed that physical activity (International Physical Activity Questionnaire score, OR = 0.998, 95% CI: 0.998–0.999, P < 0.001), body mass index (OR = 0.868, 95% CI: 0.788–0.957, P = 0.004), and inorganic phosphorus levels (OR = 0.513, 95% CI: 0.270–0.975, P = 0.042) independently influenced the likelihood of sarcopenia. Within the sarcopenic group, physical activity and BMI significantly correlated with the condition’s severity.ConclusionThe prevalence of probable or confirmed sarcopenia in patients on MHD is significant. Factors such as physical activity, body mass index, and inorganic phosphorus levels are independently associated the presence and severity of sarcopenia in this population.Trial registrationChinese Clinical Trial Registry (ChiCTR2100051111), registered on 2021–09–13.

The effect of local injections of a vitamin d receptor activator into the parathyroid glands on the level of parathyroid hormone and mineral metabolism in patients with secondary hyperparathyroidism in chronic kidney disease

Aim: to assess the dynamics of laboratory parameters (total calcium, inorganic phosphorus, albumin, and alkaline phosphatase levels) and parathyroid hormone (PTH) concentrations after administrating local injections of vitamin D receptor activators into the parathyroid glands of patients with secondary hyperparathyroidism in chronic kidney disease. The intial PTH concentration ranged from 300 to 600 pg/ml. This range was chosen to explore a more active strategy for managing the disease at its early stages and preventing the induction and progression of cardiovascular complications associated with secondary hyperparathyroidism.Methods: the study included 48 patients diagnosed with end-stage of chronic kidney disease, who were treated in the nephrology and dialysis department. The main group consisted of 34 patients who received two consecutive injections of a vitamin D receptor activator (Paricalcitol) into the most enlarged and technically accessible parathyroid gland under ultrasound guidance. The control group included 14 patients who continued with conservative treatment due to technical infeasibility of performing the injections. Effectiveness was assessed by comparing laboratory parameters before the intervention and six months after the injections in the main group, and among patients continuing standard medical therapy for secondary hyperparathyroidism.Results: the results showed a statistically significant reduction in parathyroid hormone levels after 3 and 6 months of treatment. In the control group, which continued to receive standard drug therapy, PTH and blood phosphate levels continued to rise. No undesirable effects or complications, such as hypocalcemia, bleeding, allergic reactions, and recurrent laryngeal nerve paralysis, were not observed throughout the observation period.Conclusion: this research confirms the efficacy of local injections of vitamin D receptor activators (Paricalcitol) in reducing PTH levels without significant complications or changes in calcium levels. This method could be employed to correct and prevent secondary hyperparathyroidism complications in early stages among patients with end-stage chronic kidney disease, offering a safer and more effective treatment option.

Effect of Bidens tripartita leaf supplementation on the organism of rats fed a hypercaloric diet high in fat and fructose

Herbs play an important role in folk medicine, and scientific research has confirmed the properties of their use as an alternative treatment, including the treatment and mild correction of metabolic disorders during disease. Trifid bur-marigold (Bidens tripartita) is a pharmacopoeial herbal raw material that is widely used in clinical practice as an external remedy for skin lesions and as an internal remedy for digestive and respiratory disorders. In this work, the general effect of dried leaves of B. tripartita on physiological activity and metabolic processes in model animals on a high-calorie diet was determined. For the experiment, three groups of 18 male white laboratory rats were formed and fed a hypercaloric diet (increased fat content and 20% fructose solution instead of water) for 27 days, in addition to 0.4% and 4.0% dried leaves of B. tripartita. The consumption of 0.4% and 4.0% of the medicinal plant resulted in a significant delay in the body weight gain and the average daily weight gain of the rats compared to the control group. Dried leaves of B. tripartita in the diet of rats decreased the relative weight of the thymus and increased the relative weight of the brain, and at a dose of 4.0%, increased the relative weight of the lungs and individual large intestines (cecum and colon). Dietary supplementation with B. tripartita caused an increase in globulin concentration and changes in protein coefficient. Blood parameters such as: urea, urea nitrogen, inorganic phosphorus, glucose and bilirubin levels changed depending on the dose. In the general blood test, consumption of dried leaves of B. tripartita caused a decrease in hematocrit, hemoglobin concentration and platelet count, but increased the number of eosinophils. Bidens tripartita at both concentrations significantly increased ALT activity with a corresponding change in the blood De Ritis ratio. The addition of 20 g of B. tripartita leaves to the diet increased alkaline phosphatase activity and decreased alpha-amylase activity, while 200 g increased blood gamma-glutamyltransferase activity. At the end of the experiment, the rats' orientation activity, determined in the open field, changed according to the herb dose consumed: 0.4% leaves caused an increase and 4.0% a decrease. Physical activity was reduced and emotional state increased, regardless of the dose of dried B. tripartita leaves, compared to the control group of animals. The results obtained show that the addition of B. tripartita dried leaves as a dietary supplement to a high-calorie diet is safe, does not cause pathological changes or side effects, and has a significant effect on metabolic processes. This provides theoretical support for the use of B. tripartita dried leaves in the manufacture of nutraceutical and pharmacological products for the correction of metabolic disorders in humans and animals. The doses and duration of their application require further studies.

Independent variables of pH: Ten Knights of the Hydrogen Ion Kingdom-Part I. A prospective observational study.

CO2, HCO3, SID, and total weak acids have been defined as pH's independent variables. However, according to Gamble, HCO3 should be equal to the difference between the sum of cations and the sum of anions besides HCO3. Therefore, if this mathematical expression is substituted for HCO3 in the Henderson-Hasselbalch equation, all independent variables of pH can be demonstrated. Our aim is to test this theory in this study. This prospective observational study was conducted between 2019 and 2020. All admitted patients to the intensive care unit who were >18 years old were included. Demographic data, blood gas parameters, albumin, magnesium, and inorganic phosphorus levels, and outcomes were recorded twice (at admission and at the 24th hour). The multivariate linear regression model was used to determine pH's independent variables. In the multivariate linear regression model, pH was significantly increased by each unit increase in Na, K, Ca, and Mg (mmol L-1). In contrast, pH was significantly decreased by each unit increase in CO2, Cl, lactate, albumin (g dL-1), inorganic phosphorus (mg dL-1), and the strong ion gap. Ten independent variables can accurately predict the changes in pH. For this reason, all ten independent variables should be separately evaluated when interpreting the acid-base status. With this understanding, all algorithms regarding acid-base evaluation may become unnecessary.

The Determination of Minerals in Soil, Water, Fodder, and Serum and their Effects on Reproductive Efficiency of Nili Ravi Buffaloes

Introduction: Minerals play an essential role in the reproductive performance of dairy animals, especially in buffaloes. The present study aimed to evaluate the mineral concentrations in different types of samples, including soil used for fodder cultivation, canal water used for fodder irrigation, drinking water for buffaloes, fodder used for buffalo feeding, and serum of buffaloes and their effects on the reproductive efficiency of buffaloes. Materials and methods: The current study was carried out for three months and samples were analyzed twice at the beginning and the end of the study. The concentrations of calcium, magnesium, and inorganic phosphorus in the soil were checked through the versinate method. The concentration of calcium, magnesium, and inorganic phosphorus in water was checked through the titration method and the concentration of calcium, magnesium, and inorganic phosphorus contents in fodder was checked through the wet digestion method. The minerals of blood serum (n = 80) were analyzed through commercial kits. Results: In canal water, calcium concentration was higher for irrigation compared to buffalo drinking water. Similarly, on the upper surface of the soil compared to its low surface, the level of calcium was high. Calcium values of serum were higher in cyclic buffaloes compared to buffalo heifers. In comparison to all groups, the levels of inorganic phosphorus were found to be lower in non-cyclic buffaloes. Similarly, the calcium-to-phosphorus ratio was found to be lower in non-cyclic buffaloes and heifers compared to repeat breeders and cyclic buffaloes. At the beginning of the study, cyclic buffaloes had higher magnesium levels than all other groups, except for non-cyclic buffaloes. By the end of the study, magnesium concentrations were higher in non-cyclic buffaloes and heifers compared to repeat breeders and cyclic buffaloes. Conclusion: The mineral deficiencies in water and soil can affect the fodder content in the feed of buffaloes.

VALUE OF BONE REMODELLING MARKERS IN MANDIBULAR FRACTURE PATIENTS BY GENDER AND BONE MINERAL DENSITY

Numerous studies have shown that fractures of the mandible are the most common injuries of the facial skeleton. The high incidence of such fractures is due to the anatomical and physiological characteristics of the mandible, as well as a variety of local and general causes. Disturbances in the structural-functional properties of bone tissue can occur as a result of internal and external influences and provide a favorable background for the development of fractures in various locations, including the mandible. A large number of regulatory factors of a nervous, humoral and immune nature, the presence of inflammation as an obligatory (first) phase of reparative regeneration after bone damage, dictate the need for an in-depth study of the structuralfunctional state of bone tissue in patients with traumatic injuries of the mandible, through the improvement of diagnostic and therapeutic methods. The aim of the study is to analyze the levels of bone remodeling markers in the oral fluid of patients with traumatic lesions of the mandible according to sex and BMD status compared with those of practically healthy control subjects (without general somatic and dental diseases). Materials and methods. The study is based on the results of laboratory tests on 151 people (main group) with mandibular fractures and 30 people (control group) without general somatic and dental diseases. The study included patients of both sexes aged 18-44 years: 92 (60,93%) male and 59 (39,07%) female patients. The levels of total calcium (Ca) and inorganic phosphorus (P) in the oral fluid were determined by photometric methods using commercial kits from «Filisit-Diagnostics» (Ukraine). The activity of alkaline phosphatase and acid phosphatase in oral fluid was determined by the kinetic method with n-nitrophenol phosphate. Oxyproline excretion was determined in daily urine using the Cobas test system (Roshe Diagnostics, Germany). Statistical processing of the study results was performed using Microsoft Excel and Statistics software packages for the statistical analysis of biomedical research data. Results and discussion. As a result of the studies, it was found that in men with osteoporosis, the levels of P in oral fluid were not significantly different from those in the control group, p&gt;0,05. At the same time, the remaining values of bone remodeling markers in biological fluids were characterized by a pronounced imbalance, namely an increase in Ca content by 92,85%, p&lt;0,01, p1&lt;0,05, Ca/P ratio by 2,8 times, p, p1&lt;0,01, AP activity by 89,48%, p&lt;0,01, p1&gt;0,05, ALP by 56,34%, p&lt;0,05, p1&gt;0,05, and urinary hydroxyproline level by 54,09%, p, p1&lt;0,01, compared with the same data in the control group. In women with osteoporotic mandibular fractures, the values of all the parameters studied were significantly different from those of the control group. Thus, we found an increase in the oral fluid content of Ca – by 102,38%, p, p1&lt;0,05, Ca/P ratio – by 3,5 times, p, p1&lt;0,01, AP activity – by 99,06%, p, p1&lt;0,01, ALP – by 65,15%, p, p1&lt;0,01 against a decrease in the value of P – by 42,31%, p, p1&lt;0,05, compared to the data of the control group. At the same time, in this subgroup of women there was an increase in urinary hydroxyproline by 58,26%, p, p1&lt;0,01, compared to the data in the control group. Conclusions. Thus, analysis of bone remodeling marker levels showed that in patients with mandibular fractures due to destruction of the bone matrix as a result of trauma, there was an increase in osteoresorption activity that depended on the structural-functional state of the patients' bone tissue and was more pronounced in women.

Enrichment and Bioavailability of Phosphate in a Highly Urbanized Zone of Cochin Estuary, South India

ABSTRACT Sequential extraction (SEDEX method) was employed to explore the distribution of sediment-bound phosphorus (P) fractions and the bioavailability of P in Cochin estuary. Concentration of P fractions in surface sediments of the study area ranged as follows: Detrital P > Organic P > Authigenic P > Exchangeable P > Iron-bound P. Generally increasing trend was displayed by all the phosphate fractions toward the downstream sites due to effluent discharges from industrial, domestic, municipal, agricultural sectors and destruction of mangrove forests. The elevated levels of inorganic phosphorus (133.55–918.73 mg/kg) reflected the large-scale delivery of sediment loads associated with great floods during 2018 & 2019. Significant correlations of fine-grained sediment particles with all phosphate fractions indicated surface adsorption as a dominant distribution mechanism. Concentration of iron-bound phosphorus was controlled by adsorption on iron oxy-hydroxides. The buildup of bioavailable phosphate (range: 23.76 to 49.97%) indicate the fact that the estuarine sediments function as a sink for the nutrient. The upper and middle part of the study area recorded more severe contamination and eutrophication risk as indicated by phosphorous pollution index (range: 0.31 to 2.56).

Association of Circulating Endothelial Nitric Oxide Synthase Levels with Phosphataemia in Patients on Haemodialysis.

The amount of evidence indicates that hyperphosphataemia (HP) can induce endothelial damage and significantly impair endothelial nitric oxide synthase (eNOS) expression. There are no clinical studies that have assessed HP and its correlation with circulating eNOS concentration in patients with end-stage renal disease (ESRD). Our preliminary study aimed to evaluate the relationship between plasma inorganic phosphorus (P) levels and circulating plasma eNOS concentration in patients on haemodialysis (HD). A total of 50 patients on HD were enrolled to the study. They were divided into groups according to the tertiles of P. The examined HD group was also analysed and compared with controls as a whole group; then, the group was divided into patients with and without dyslipidaemia (D) as well as into those with and without type 2 diabetes mellitus (type 2 DM). A total of 26 age-matched healthy volunteers were included in the study as the control group. The plasma levels of eNOS in HD patients are reduced in comparison to those in healthy subjects. There was no difference in plasma eNOS concentrations between HD patients with type 2 DM and those without DM as well as between those with D and without D. In the entire group of HD patients, there were positive correlations between circulating levels of eNOS and plasma P concentrations. In HD patients with D, higher systolic and diastolic blood pressure were accompanied by decreased plasma eNOS concentrations. In conclusion, HP and high blood pressure appear to decrease the circulating eNOS levels. These findings demonstrate an additional negative impact of HP on eNOS activity.

Effectiveness of complex treatment of cats for chronic kidney disease

Treatment of cats with chronic kidney disease should be aimed at slowing the progression of the disease, which contributes to the preservation of residual kidney function, the elimination of clinical signs of CKD, and the quality of life of sick animals. During the II stages of chronic kidney disease in cats, hyporexia (60 %), polyuria and polydipsia (50 %), anemia of mucous membranes (60 %), impaired coordination of movements (30 %), vomiting and ulcerative stomatitis (40 %), an increase in serum levels of creatinine and urea (P &gt; 0.001), the level of symmetric dimethylarginine (P &lt; 0.001), the concentration of cystatin C (P &gt; 0.001), a decrease in the rate of glomerular filtration, the level of total calcium (P &lt; 0.001), an increase in the level of inorganic Phosphorus and Potassium. Arterial hypertension was established in 8 cats; the risk was moderate (148 ± 4.7 / 98 ± 3.8 mmHg). Changes in the physical properties and chemical composition of urine have been established. The application of complex treatment of cats with II stages of chronic kidney disease for 14 days contributed to the restoration of appetite, increased motor activity, the absence of anemia of visible mucous membranes and vomiting, and a decrease in the level of polyuria and polydipsia was established. Hematological indicators improved, the content of symmetric dimethylarginine in blood serum (Р &lt; 0.001), Cystatin C (Р &lt; 0.001), the level of total calcium (Р &lt; 0.001) normalized, azotemia decreased (Р &lt; 0.001), the rate of glomerular filtration increased (Р &lt; 0.001), the level of inorganic Phosphorus and Potassium (Р &lt; 0.001). An increase (Р &lt; 0.001) in the relative density of urine and a decrease in the content of protein and creatinine were noted in the urine. For arterial hypertension in cats with chronic kidney disease, life-long therapy is indicated, which is adjusted according to the needs and condition of the animal.

Assessment of calcium, phosphorus, magnesium, vitamin D and PTH levels in sera of lame horses.

Minerals play vital roles in physiologic processes. Calcium, phosphorus and magnesium are common minerals. Secretion, absorption and homeostasis of these minerals are affected by associations between the active vitamin D and parathyroid hormone (PTH). The aim of this study was to assess concentrations of vitamin D, PTH and minerals, such as calcium, phosphorus and magnesium in horse sera, as well as associations of these values with lameness in horses. In the references, the reasons for lameness can be deficiency and imbalance of minerals, and clinical observations also confirm this and there is still not enough information regarding the relationship between mineral imbalance and lameness of horses. The prominent forms of lameness include navicular syndrome, back pain, splints, ring bone, side bone, non-infectious arthritis and bucked shins. Deficiency and inadequacy of calcium, phosphorous and magnesium have been regarded as predisposing and aggravating factors of the aforementioned diseases. This cross-sectional study was carried out on 60 horses, including 30 lame and 30 healthy adult horses, summer to fall 2021. Levels of calcium, phosphorus, magnesium, vitamin D and PTH were assessed in sera using automatic analyser, atomic absorption spectroscopy and commercially available kits. The average level of calcium was higher healthy horses than lame horses in the two seasons (p<0.001). The average level of inorganic phosphorus in horses was higher in summer than autumn (p<0.001). The mean magnesium concentration in healthy horses was greater than lame horses in the two seasons (p<0.01). In this study, the mean PTH concentration in healthy horses was lower in fall than summer (p<0.05). The low average levels of calcium and magnesium in lame horses in the two seasons indicate critical roles of calcium and magnesium in the normal function of the horse musculoskeletal system as well as prevention of lameness.

Productive, biochemical and morphological blood parameters of piglets for fitobiotic feed additive “Activo’’ feeding

The article presents data on the effect of the phytobiotic additive “Activo” at a dose of 0.2 g/kg of feed on the piglets live weight of the growing and finishing fattening periods. The average daily gain and feed consumption per 1 kg of weight gain were determined during the experiment. Among biochemical parameters it was determined the level of total proteins, protein fractions (albumin and globulins), calcium and inorganic phosphorus. The level of morphological parameters in the blood of piglets of both fattening periods was determined. In particular, the content of hemoglobin, erythrocytes, leukocytes, platelets, eosinophils, rod-shaped and segmented granulocytes, lymphocytes, monocytes and hematocrit was determined. As a result of the research, it was found that the addition of a phytobiotic feed additive in the amount of 0.2 g/kg to the growing and finishing feed increased live weight by 8.12 and 3.36 %, respectively. At the same time, feed consumption per 1 kg of weight gain was reduced in the experimental group of piglets of the growing and finishing periods by 8.84 and 10.95 %, respectively. Phytobiotic additive at a dose of 0.2 g/kg of feed influenced the level of biochemical and morphological parameters in the piglets’ blood of the growing and finishing fattening periods. In particular, in the experimental group of piglets of the growing period, the level of total protein was higher by 5.25 %, and in the finishing period, it was lower by 7.60 %. Morphological parameters in the blood of piglets of the growing and finishing fattening periods of the experimental group had a higher level of hemoglobin, red blood cells and leukocytes. In piglets of the experimental group in the final period of fattening, the level of platelets, eosinophils and hematocrit was higher.

Features of coronary calcification in patients receiving renal replacement therapy with program hemodialysis

Objective: to study the features of coronary calcification in patients with chronic kidney disease receiving renal replacement therapy with program hemodialysis. Materials and methods: in the process of an open clinical trial, a group of patients (n = 43) with end-stage chronic kidney disease (23 women and 20 men) was formed. The average age of patients was 54±13 years, the median length of dialysis therapy was 11 [6; 16] months. All patients received renal replacement therapy with program hemodialysis. The procedures were carried out on artificial kidney devices according to the standard scheme 3 times a week for 4 hours. Patients underwent traditional clinical and laboratory examination. All patients were assessed for coronary calcification by multispiral computed tomography. On the Agatston scale, the severity of coronary calcification was assessed taking into account the density and area of calcium deposition. Results: on the results of multispiral computed tomography to determine the degree of calcification of the coronary arteries, we found that 48% of patients had low and minimal calcification, 16% — insignificant calcification, 36% — measured and pronounced calcification. When comparing laboratory parameters in the groups of patients with different calcification of the coronary arteries, the level of inorganic phosphorus (1,64±0,07 mmol/L) was significantly higher in patients with insignificant, moderate and severe calcification than in patients with minimal and low calcinosis (1,35±0,12 mmol/L) (p=0.04). There was a pronounced direct correlation between age and calcium index (r=0,52, p=0,0011). With an increase in dialysis experience, an increase in calcium index is noted (r = 0,14; p = 0,045). Conclusion: in 52% of patients with end-stage chronic kidney disease receiving renal replacement therapy with program hemodialysis, coronary calcification is determined from the results of multispiral computed tomography of the coronary arteries. Patients with moderate to severe coronary calcinosis have significantly higher levels of serum phosphorus compared to patients with minimal to low calcinosis. At the same time, the age of patients and the length of dialysis therapy affect the severity of coronary calcinosis

Cyanometallate framework templated synthesis of hierarchically porous La(OH)3 for High-Efficient and stable phosphorus removal from tailwater

Lanthanum (La)-based hydroxides have been recognized as promising candidates for dephosphorization due to their high affinity and selectivity for phosphate adsorption. However, the development of porous La(OH)3 materials with enriching active sites and high structural stability remains a challenge in tailwater treatment. Here we developed a novel self-templating method based on an ion-exchange mechanism to construct hierarchically porous La(OH)3, where the OH− of an alkali conversion reagent replaces the [Fe(CN)6]4− cluster within the LaFe cyanometalate (CM) precursor, simultaneously completing the hydroxide conversion and pore-creating processes. The obtained hierarchically porous La(OH)3 shows excellent structural stability with no ion leaching and can maintain its primary microsphere structure and secondary nanoneedle morphology after multiple cycles of application. Due to the abundant micropores/mesopores, there are fully exposed active sites inside and outside the porous La(OH)3 microsphere, giving it an excellent phosphate adsorption capacity (maximum capacity of 163.6 mg P/g). Its high selectivity and environmental adaptability make it a favorable adsorbent for aquaculture tailwater treatment that can rapidly reduce the dissolved inorganic phosphorus level to below 0.05 mg P/L to meet the mariculture tailwater discharge standard in a wide range of salinities and temperatures. The information gleaned in this study provides valuable guidance for the materials design of porous La-based hydroxides for phosphorus removal.

Оценка обеспеченности витамином D, кальцием и фосфором лиц, проходящих службу в условиях Арктики

Inadequate ultraviolet insolation is one of the the key prerequisites for the pathogenesis of body's vitamin D insufficiency in the North. The study was aimed to assess the body's vitamin D, calcium and phosphorus sufficiency in the contract servicemen deployed in Arctic. The contract servicemen deployed on the Cape Chelyuskin and Dixon Island were surveyed (n = 51). The serum levels of 25(OH)D, the intermediate of the vitamin D conversion, along with the ionized calcium, total calcium, and inorganic phosphorus levels, were determined in June. Three degrees of the vitamin D sufficiency were revealed in the military, who had been deployed in Arctic for 5.9 ± 0.4 years: deficiency (in 29.4%), insufficiency (in 52.9%), and optimal levels (in 17.7%). However, the optimal levels revealed were close to the lower limit of normal range. Low ionized calcium levels were found in 29.4% of blood samples (15.5 ± 0.6 ng/mL). A total of 70.6% of samples that were within normal range were close to the lower limit of normal range based on Q25 (1.16 mmol/L) and were within the lower half of normal range (1.15–1.35 ng/mL) based on Q75 (1.22 mmol/L). The measured total calcium and inorganic phosphorus levels were close to the lower limits of reference ranges (2.29 ± 0.009 and 0.83 ± 0.006 mmol/L, respectively). In general, the reduced ionized calcium levels associated with vitamin D insufficiency were revealed, which were indicative of impaired calcium metabolism. The vitamin D deficiency results from the total calcium and inorganic phosphorus concentrations that are close to lower limits of reference ranges. Further negative changes in the body's vitamin D, phosphorus and calcium sufficiency should be expected during polar night. The study actualizes the year-round replenishement of the vitamin D and mineral deficiency in the military.

A RETROSPECTIVE STUDY OF CORRELATION BETWEEN SERUM CREATININE, SERUM CALCIUM, SERUM INORGANIC PHOSPHORUS AND SERUM MAGNESIUM LEVELS IN PATIENTS ATTENDING NEPHROLOGY CLINICS IN TERTIARY CARE CENTER

Introduction: Chronic kidney disease (CKD) has been recognized as a leading public health problem worldwide. The global estimated prevalence of CKD is 13.4%. Kidney plays a critical role in regulating serum levels of Calcium, Magnesium and Phosphate. Calcium, phosphorus and magnesium homeostasis is altered in chronic kidney disease(CKD). Objective Objective: of the study is to establish the correlation of increase in serum creatinine levels with Calcium, Magnesium and Phosphate levels in patients. Design &amp; Methods: This observational retrospective study included patients attending nephrology department in tertiary care centre for a period of two months. In total, 229 patients' data was accessed from the medical records. Patients were grouped in 5 groups based on serum Results: creatinine levels. Group I with serum creatinine levels of 0.5mg/dl to 0.8mg/dl, group II with serum creatinine levels of 0.8 to 1.1mg/dl, group III with serum creatinine levels of 1.1 to 3mg/dl, group IV with serum creatinine levels of 3 to 6mg/dl, group V with serum creatinine levels above 6mg/dl. Overall from group I to Group V , Serum Calcium showed negative and signicant correlation, Serum phosphorus levels showed positive and signicant correlation, Serum magnesium showed negative and insignicant correlation with serum creatinine. As Conclusions: serum creatinine increases, more patients show decline in serum calcium levels and serum magnesium levels and increase in serum inorganic phosphorus levels. Hypocalcemia, hypomagnesemia and hyperphosphatemia have been associated with increased risk of cardiovascular morbidity and mortality in cases of CKD. Hypomagnesemia has possible association with rapid decline in kidney function. Therefore, regular analysis of these parameters is important for predicting the prognosis of CKD and cardiovascular risk in cases of CKD.

Biochemical parameters of blood in cats with cardiogenic arterial thromboembolism and acute heart failure

Investigating the impact of vascular blockage on blood biochemical parameters, particularly in the context of acute heart failure, is crucial for understanding the underlying mechanisms of arterial thromboembolism in animals. The purpose of the study is to investigate the effect of vascular occlusion on the biochemical parameters of blood in cats with cardiogenic arterial thromboembolism. The study involved 12 cats and formed two groups: the first – animals with acute heart failure and the second – animals with arterial thromboembolism. Anamnesis data were collected for experimental animals, clinical examination, echocardiography, and biochemical examination of blood samples were performed. The activity of alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase, the concentration of glucose, creatinine, urea, potassium, calcium, total and inorganic phosphorus were determined spectrophotometrically in blood serum. It was found that in cats with acute heart failure, the average value of serum aspartate aminotransferase activity increased by 2.8 times, and the creatinine concentration by 1.9 times compared to the upper limit of reference values. This indicates functional changes in the myocardium and kidneys in this pathology. In cats affected by arterial thromboembolism, the blood serum shows a significant elevation in various biochemical parameters compared to the upper limit of the corresponding reference intervals. Specifically, there is a 4.4-fold increase in alanine aminotransferase activity, a 4.3-fold increase in aspartate aminotransferase activity, a 1.4- fold increase in glucose concentration, a 1.8-fold increase in creatinine levels, a 2.0-fold increase in urea levels, and a 1.3-fold increase in inorganic phosphorus levels. The changes in biochemical parameters for feline thromboembolism may be a consequence of functional renal failure, ischemia, and muscle necrosis. Moreover, it was established that in most cats with acute heart failure, the urinary system is primarily affected, and with arterial thromboembolism, changes in muscle tissue also occur. The results obtained are of practical value for veterinary doctors when choosing a therapeutic strategy and prescribing additional diagnostic examinations

Clinical and Pathogenetic Significance of Amylase Level and Microtomographic Index of Synovial Fluid in Various Joint Lesions.

Samples of synovial fluid from 95 patients with various joint pathologies at the stage of the disease progression characterized by copious effusion into articular cavities have been examined. Synovial fluid samples obtained by knee arthrocentesis served as a material for the investigation. Conventional methods were used to determine the concentration of uric acid, inorganic phosphorus, total protein, and amylolytic activity level in the selected samples while X-ray density was identified by computed microtomography. All samples of pathological joint fluid have shown a high level of amylolytic activity as compared to the synovial fluid from healthy joints. The relationship between the level of amylolytic activity in synovia and specific joint pathology has been identified. It has also been found that uric acid values, inorganic phosphorus concentrations, and total protein in various types of joint damage may influence X-ray density of the synovial fluid. Correlations between the studied indices have been established. New data on the level of synovia amylolytic activity has been obtained in one non-inflammatory and six different inflammatory diseases. Pathogenically determined correlation between the microtomographic index of synovial fluid density and concentrations of uric acid, inorganic phosphorus, total protein has been confirmed. Specific indicators of X-ray density of synovia in various joint pathologies as well as unidirectional and multidirectional data in comparison with the norm allow us to consider X-ray microtomography as a method that reveals additional details during investigation of synovial fluid density and brings new surrogate markers for the study of pathogenetic mechanisms of the development, differentiation, and treatment of various joint pathologies.

Simultaneous adsorption removal of organic and inorganic phosphorus from discharged circulating cooling water on biochar derived from agricultural waste

A high level of organic and inorganic phosphorus exists in discharged circulating cooling water (DCCW), therefore, the efficient removal of phosphorus from DCCW is necessary. Herein, the adsorption performance and mechanism of hydroxyethylidene diphosphonic acid (HEDP) and phosphate from DCCW on pyrolysis biochar derived from agricultural waste (wheat and corn straw wastes, and peanut shell powder) were systematically investigated. Among them, biochar prepared from wheat straw and modified with MgCl2 and CeCl3 (molar ratio 3:1), i.e., WS-3Mg/Ce, showed optimal phosphorus removal from DCCW. The pseudo-second-order and Freundlich model well described the adsorption of HEDP, PO43−, and total phosphorus (TP) on WS-3Mg/Ce. Further, negative Gibbs free energy (ΔG) indicated that WS-3Mg/Ce adsorbed HEDP and phosphate through physical adsorption and chemical reactions, with the chemical reaction being dominant. Scanning electron microscopy-energy dispersive X-ray spectroscopy and X-ray diffraction results revealed that CePO4 and Mg3(PO4)2 appeared on WS-3Mg/Ce after adsorption, and the diffraction bands of metal oxides or hydroxides decreased. Fourier transform infrared and X-ray photoelectron spectroscopy results showed that O–H, M-O, M-O-P, and O–P on the surface of WS-3Mg/Ce participate in the reaction. This suggests that the adsorption of HEDP and phosphate on WS-3Mg/Ce mainly involves metal binding via Ce and Mg, and chemical complex via OH−. This study provides a promising approach for removing different forms of phosphorus simultaneously from DCCW via biochar and contributes to the resource recovery of agricultural and forestry waste. Future work is needed for the optimization of the biochar to promote the adsorption capacity and renewability in treating real DCCW.

USE OF SOME MEDICAMENTS IN THE TREATMENT OF AFFECTED CALVES WITH HYPOMAGNESAEMIC TETANY

This trial comprised (28) naturally affected calves with hypomagnesaemic tetany, which were diagnosed clinically and by measuring the serum level of magnesium, calcium, sodium, inorganic phosphorus, potassium and SGOT &amp; CPK enzymes. There were significant decrease (P&lt;0.01) in serum level of magnesium and calcium. While serum potassium and serum enzymes level shown significant increase (P &lt; 0.01) during tetanic attacks. On the other hand serum sodium and inorganic phosphorus level were dropped slightly. Each calf was treated for three successive days with magnesium and calcium salts, with tranquilizers, in addition to sodium chloride for relief of dehydration. With the use of these medicaments as compared to the commerical preparations, the frequency of tetanic attacks decreased gradually then disappeared completely after the completion of treatment.

Eukaryotic Microalgae and Cyanobacterial Diversity Analysis from Bharathidasan University Campus, Tamil Nadu, India

Eukaryotic microalgae and Cyanobacteria have immense biodiversity, and are an almost unexploited resource. They have been identified as a hopeful and commercially valuable resource of high-value products such as proteins, fatty acids, pigments, vitamins, alkaloids, flavonoids and steroids etc., in the food and aquaculture industries. Microalgae are a promising candidate for the production of useful products due to their low cost biomass production, ease of cultivation, and glycosylation patterns similar to that of higher plants. Though quite a few species of microalgae have been identified as a potential candidate to fulfill our needs, remain unexplored. The aim of the present study is to enumerate the microalgal diversity in the vicinity of Bharathidasan university (Tiruchirapalli, TamilNadu, India). Physicochemical parameters of the water samples were also determined.the pH of the sites varied from 6.5 to 7.4, lkalinity was between 24 mg L-1 and 42 mg L-1, total alkalinity was between 312 mg L-1 and 681 mg L-1, calcium, magnesium, chloride and nitrate levels varied between 329 mg L-1and 1318 mg L-1, 220 mg L-1 and 826 mg L-1, 12.99 mg L-1 and 193.33 mg L-1, 4.0 mg L-1 and 9.8 mg L-1, respectively. Nitrite, total phosphorus and inorganic phosphorus levels were found to be between 2.1 mg L-1 and 4.3 mg L-1, 2.4 mg L-1 and 6.7 mg L-1, and 1.2 mg L-1 and 4.3 mg L-1. Biodiversity of both eukaryotic microalgae and cyanobacteria recorded the members of the belonging to Oscillatoria sp. are predominant in diversity and distribution followed by other taxa such as Spirulina laxissima, Staurastrum manfeldtii, Merismopedia elegans, Cosmarium blyttii and Cosmarium subcucumis.