Level Of SA Research Articles

Deciphering Whether Illite, a Natural Clay Mineral, Alleviates Cadmium Stress in Glycine max Plants via Modulation of Phytohormones and Endogenous Antioxidant Defense System

Globally, cadmium (Cd) stress dramatically reduces agricultural yield. Illite, a natural clay mineral, is a low-cost, environmentally acceptable, new promising method of reducing the heavy metal (HM) stress of cereal crops. In research statistics, there is little research on stress tolerance behavior of Illite (IL) on an experimental soybean plant. In the present study, we took IL and examined it for tolerance to Cd, as well as for other plant-growth-promoting (PGP) characteristics in Glycine max (soybean). The results showed that applying clay minerals in different concentrations enhanced the level of SA (defense hormone) and reduced the level of ABA (stress hormone). Cd 1 mM significantly reduces plant growth by altering their morphological characteristics. However, the application of IL significantly enhanced the seedling characteristics, such as root length (RL), 29.6%, shoot length (SL), 14.5%, shoot fresh biomass (SFW), 10.8%, and root fresh biomass (RFB), 6.4%, in comparison with the negative control group. Interestingly, IL 1% also enhanced the chlorophyll content (C.C), 15.5%, and relative water content (RWC), 12.5%, in all treated plants. Moreover, it resulted in an increase in the amount of superoxide dismutase (SOD), phenolics, and flavonoids in soybean plants, while lowering the levels of peroxidase (POD) and H2O2. Furthermore, compared to control plants, soybean plants treated with the Illite exhibited increased Si absorption and lower Cd levels, according to inductively coupled plasma mass spectrometry (ICP-MS). Thus, the IL can operate as an environmentally beneficial biofertilizer and sustainable approach under Cd stress by promoting plant development by activating signaling events.

Integrating histology and phytohormone/metabolite profiling to understand rooting in yellow camellia cuttings

Vegetative propagation through cutting is a widely used clonal approach for maintaining desired genotypes. However, some woody species have difficulty forming adventitious roots (ARs) with this approach, including yellow camellia (YC) C. nitidissima. Yellow camellias, prized for their ornamental value and potential health benefits in tea, remain difficult to propagate clonally due to this rooting recalcitrance. As part of the efforts to understand YC cuttings’ recalcitrance, we conducted a detailed investigation into AR formation in yellow camellia cuttings via histology and endogenous phytohormone dynamics during this process. We also compared YC endogenous phytohormone and metabolite phytohormone profiles with those of easy-to-root poplar and willow cuttings. Our results indicate that the induction of ARs in YC cuttings is achievable through auxin treatment, and YC ARs are initiated from cambial derivatives and develop a vascular system connected with that of the stem. During AR induction, endogenous hormones showed a dynamic profile, with IAA continuing to increase starting 9 days after auxin induction. JA, JA-Ile, and OPDA showed a similar trend as IAA but decreased by the 45th day. Cytokinin first decreased to its lowest level by the 18th day and then increased. SA largely exhibited an increasing trend with a drop on the 36th day, while ABA first increased to its peak level by the 18th day and then decreased. Compared to poplar, YC cuttings had a low level of IAA, IAA-Asp, and OPDA, and a high level of cytokinin and SA. Metabolite profiling highlighted significant down-accumulation of compounds associated with AR formation in yellow camellias, such as citric and ascorbic acid, fructose, sucrose, flavonoids, and phenolic acid derivatives. Our study reveals the unfavorable endogenous hormone and metabolite profiles underlying the rooting recalcitrance of YC cuttings, providing valuable knowledge for addressing this challenge in clonal propagation.

Magnaporthe oryzae effector AvrPik-D targets a transcription factor WG7 to suppress rice immunity

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases for rice crops, significantly affecting crop yield and quality. During the infection process, M. oryzae secretes effector proteins that help in hijacking the host's immune responses to establish infection. However, little is known about the interaction between the effector protein AvrPik-D and the host protein Pikh, and how AvrPik-D increases disease severity to promote infection. In this study, we show that the M. oryzae effector AvrPik-D interacts with the zinc finger-type transcription factor WG7 in the nucleus and promotes its transcriptional activity. Genetic removal (knockout) of the gene WG7 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after treatments with chitin. In addition, the hormone level of SA and JA, is increased and decreased respectively in WG7 KO plants, indicating that WG7 may negatively mediate resistance through salicylic acid pathway. Conversely, WG7 overexpression lines reduce resistance to M. oryzae. However, WG7 is not required for the Pikh-mediated resistance against rice blast. In conclusion, our results revealed that the M. oryzae effector AvrPik-D targets and promotes transcriptional activity of WG7 to suppress rice innate immunity to facilitate infection.

Optical simulations of the impact of vault increase in scleral contact lenses in healthy eyes

PurposeTo investigate by using computational simulations the optical impact of the change in the vault of two geometries of scleral contact lenses (SCLs). MethodsRay-tracing simulations were performed using specialized software in three eye models with different levels of primary SA (6 mm pupil). Two different geometries of SCL were used in such simulations characterized by the conic constants of the anterior surface of the lens (K1, −0.1 and −0.3). Likewise, the fitting of the SCL was simulated for different vaults (50–250 µm). The impact on the quality of the images through the eye models was assessed by analyzing the modulation transfer function (MTF) at different spatial frequencies (10 Lp/mm, 30 Lp/mm, and 50 Lp/mm). This impact was not only simulated for a distant object, but also for intermediate and near objects (vergence demands from 0.00 to 3.00 D). All these optical simulations were performed assuming a centered SCL, but also assuming a downward vertical decentration of 0.5 mm. ResultsThe thinnest vault (50 µm) provided the best ocular optical quality in all three eye models for low vergence demands. For medium and high vergence demands, Lens 1 (K1 = −0.3, K2 = −0.4) resulted in a considerable improvement in optical quality in Eye 2 (C40 = −0.078 µm), while for eyes 1 (C40 = 0.408 µm) and 3 (C40 = −0.195 µm), this improvement only tended to happen for medium vergence demands. Overall, all the aberrations increased after lens fitting. Lens decentration did not cause significant variations in the results obtained with the well-centered lenses. ConclusionsChanges in the vault of a SCL have an impact on the optical quality achieved for different vergence demands independently on the level of SA of the eye in which it is fitted. The clinical relevance of such impact should be investigated further.

Exogenous melatonin induces phenolic compounds production in Linum album cells by altering nitric oxide and salicylic acid

Melatonin is a pleiotropic molecule that can influence various aspects of plant performance. Recent studies have exhibited that it mediates plant defensive responses, probably through managing redox homeostasis. We tried to track the regulatory effects of melatonin on the antioxidant machinery of Linum album cell culture. To this, different concentrations of melatonin were applied, and the oxidative status of cells was investigated by measuring the levels of oxidative molecules and antioxidant agents. The results showed that H2O2 content did not change at the low melatonin levels, while it increased at the high concentrations. It can be correlated with the low melatonin dosages capacity to remove excessive amounts of H2O2, while the high melatonin dosages exhibit toxicity effects. In contrast, the NO enhancement occurred at 50 μM melatonin, proposing its role in triggering melatonin-induced defensive responses. The MDA results stated that NO led to oxidative stress in melatonin-treated cells at 50 μM melatonin. Antioxidant enzyme POD was activated by melatonin treatment, while SOD enzyme behaved reversely which can explain the changes in the H2O2 level. In addition, the analysis of the phenolics profile showed that the contents of phenolic acids, flavonoids, and lignans enhanced following an increase in PAL enzyme activity. The increased level of phenolic hormone SA can indicate that melatonin affects the defensive responses in L. album cells through a SA-dependent pathway. In general, it seems that melatonin, by modulating NO and SA levels, can induce the activity of antioxidant enzymes and the production of phenolics, especially lignans, in L. album cells.

Effects of Dietary Sodium Acetate on Growth Performance, Fillet Quality, Plasma Biochemistry, and Immune Function of Juvenile Golden Pompano (Trachinotus ovatus)

Six isonitrongenous and isolipidic diets were formulated to feed 360 fish (mean initial body weight: 6.06 ± 0.02 g) with graded levels of sodium acetate (SA1 (0), SA2 (250 mg/kg), SA3 (500 mg/kg), SA4 (1000 mg/kg), SA5 (2000 mg/kg), and SA6 (4000 mg/kg)) for 8 weeks. The results revealed that weight gain rate, specific growth rate, and feed intake of golden pompano were significantly increased in SA3 and SA4 treatments ( p < 0.05 ). Dietary SA level significantly improved lipid contents and gumminess of muscle ( p < 0.05 ). Supplement levels of dietary SA significantly influenced plasma biochemical parameters containing triglyceride, glucose, high-density lipoprotein cholesterol, complements, and immunoglobulin M ( p < 0.05 ). Appropriate supplement levels of dietary SA significantly increased enzyme activities of hepatic superoxide dismutase and total antioxidant capacity and decreased malondialdehyde contents ( p < 0.05 ). Furthermore, appropriate SA levels downregulated the expression levels of Keap1, IL-8, TNF-α, and NF-κB genes and upregulated the expression levels of Nrf2 and TGF-β genes of the spleen ( p < 0.05 ). Excess SA caused negative effects for Trachinotus ovatus. The optimum supplement level of SA for juvenile golden pompano was evaluated to be 1423.67 mg/kg based on WGR.

Statistics Anxiety in Flanders: Exploring Its Level, Antecedents, and Performance Impact Across Professional and Academic Bachelor Programs in Psychology

The current study focusses on the level of statistics anxiety and the motivation to learn statistics in Flanders (Belgium) and determined to what degree these factors and their interaction relates to statistical performance. For this purpose, the Statistics Anxiety Scale and the Statistics Motivation Scale were translated, validated, and administered in professional and academic bachelor students in psychology. The level of SA in Flanders is comparable to other countries, with professional bachelor students being more anxious to make interpretations compared to academic bachelor students, who in turn are more anxious to ask for help. Academic students are more motivated to learn statistics compared the professional bachelor students, mostly in terms of intrinsic motivation. The overall motivation to learn statistics is lower at the end of the semester compared to the beginning of the semester. This is unfortunate, because we observed that high levels of motivation can alleviate the negative impact of statistics anxiety on statistical performance, especially when controlling for general learning abilities.

Nitric oxide-mediated alleviation of arsenic stress involving metalloid detoxification and physiological responses in rice (Oryza sativa L.)

Rice is a staple crop, and food chain contamination of arsenic in rice grain possesses a serious health risk to billions of population. Arsenic stress negatively affects the rice growth, yield and quality of the grains. Nitric oxide (NO) is a major signaling molecule that may trigger various cellular responses in plants. The protective role of NO during arsenite (AsIII) stress and its relationship with plant physiological and metabolic responses is not explored in detail. Exogenous NO, supplemented through the roots in the form of sodium nitroprusside, has been shown to provide protection vis-à-vis AsIII toxicity. The NO-mediated variation in physiological traits such as stomatal density, size, chlorophyll content and photosynthetic rate maintained the growth of the rice plant during AsIII stress. Besides, NO exposure also enhanced the lignin content in the root, decreased total arsenic content and maintained the activities of antioxidant isoenzymes to reduce the ROS level essential for protecting from AsIII mediated oxidative damage in rice plants. Further, NO supplementation enhanced the GSH/GSSG ratio and PC/As molar ratio by modulating PC content to reduce arsenic toxicity. Further, NO-mediated modulation of the level of GA, IAA, SA, JA, amino acids and phenolic metabolites during AsIII stress appears to play a central role to cope up with AsIII toxicity. The study highlighted the role of NO in AsIII stress tolerance involving modulation of metalloid detoxification and physiological pathways in rice plants.

In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.)

Fig tree (Ficus carica L.) is a precious fruit tree in semi-arid and arid areas worldwide which has difficulties in its conventional breeding programs. This study was carried out to make new genotypes with superior features based on the ploidy induction method. Thus, in vitro tetraploidization in two fig cultivars, namely ‘Sabz’ and ‘Torsh’, was successfully established using shoot tip explants and colchicine as the antimitotic agent in MS medium. The flow cytometry and chromosome counting techniques were used to verify tetraploid plants. The results revealed that, in comparison to the original diploid plants of both cultivars, tetraploid plants significantly had taller stems, larger leaves, a greater number of chloroplasts in guard cells, and higher chlorophyll content and photosynthesis rate. UPLC-MS analysis revealed that the level of growth stimulator phytohormones, including ZR, IAA, GA3, SA, and JA in the tetraploid plants of both cultivars were significantly higher than their diploid controls. In contrast, they had less accumulated growth inhibitor phytohormone (ABA) than their diploid explant source. Moreover, tetraploid plants had significantly accumulated a higher content of phenolic compounds, total soluble sugars, and total soluble proteins, but showed a significantly less total antioxidant activity. Consequently, it is concluded that the growth advantages of tetraploid figs created in this study are substantial in terms of phytohormonal, physiological, and phytochemical superiorities, as compared to their corresponding diploid plants. Polyploidization proves as a promising breeding tool for future breeding programs of the fig tree.

Biochemical Confirmation of the Anti-Inflammatory Effect of Coxibs and their Compositions with N-Acetylgulcosaminil 1-4-N-Acetylmuramoil-L-alanyl-D- Isoglagamin

The current work is focused on the biochemical confirmation of the anti-inflammatory effect of coxibs and their compositions with licopid as an adjuvant for NSAIDs in the treatment of inflammation. The current investigation aimed to compare the anti-inflammatory effect of rofecoxib, celecoxib, licopid, and pharmaceutical compositions consisting of rofecoxib and licopid, celecoxib, and licopid. Sodium diclofenac was chosen as the reference drug. The anti-inflammatory effect of the test substances was studied using an experimental model of formalin-induced paw edema. The level of SA was determined using SialoTest (SPC Eco-Service). A biochemical study of the anti-inflammatory effect of rofecoxib, celecoxib, licopid, and their composition on the content of inflammation marker (SA) showed that almost all studied drugs reduced the content of SA in rat’s blood serum compared to the negative control. The results of the biochemical study showed that rofecoxib has a pronounced anti-inflammatory effect. It acts almost 1.2 times better than celecoxib, and the leader in our biochemical studies is a two-component pharmaceutical composition of celecoxib + licopid at the level of SA in blood serum under formalin-induced edema.

Multiparameter flow cytometric detection and quantification of senescent cells in vitro

It has been over half a century since cellular senescence was first noted and characterized, and yet no consensus senescent marker has been reliably established. This challenge is compounded by the complexity and heterogenic phenotypes of senescent cells. This necessitates the use of multiple biomarkers to confidently characterise senescent cells. Despite cytochemical staining of senescence associated-beta-galactosidase being a single marker approach, as well as being time and labour-intensive, it remains the most popular detection method. We have developed an alternative flow cytometry-based method that simultaneously quantifies multiple senescence markers at a single-cell resolution. In this study, we applied this assay to the quantification of both replicative and induced senescent primary cells. Using this assay, we were able to quantify the activity level of SA β-galactosidase, the expression level of p16INK4a and γH2AX in these cell populations. Our results show this flow cytometric approach to be sensitive, robust, and consistent in discriminating senescent cells in different cell senescence models. A strong positive correlation between these commonly- used senescence markers was demonstrated. The method described in this paper can easily be scaled up to accommodate high-throughput screening of senescent cells in applications such as therapeutic cell preparation, and in therapy-induced senescence following cancer treatment.

Level of biogenic amines for pregnant women with psycho-emotional disorders stipulated by anxiety

The objective: determine level of biogenic amines for pregnant women with psycho-emotional disorders stipulated by anxiety. Materials and methods. 90 Pregnant women were examined in ІІ and ІІІ trimester of pregnancy. The main group includes 58 pregnant women with the medium and high level of state anxiety and 32 pregnant women with anxiety level of 30 points and less that indicates the low level of SA (control group). For estimation of the state anxiety and trait anxiety the scale of Spielberger-Hanin was used. Level of serotonin and melatonin was measured with fluorometric method in blood hemolysate of pregnant women. Results. According to the obtained results of biochemical research of hemolysate of packed red cells of blood of examined women there was stated that increase of state anxiety was accompanied by statistically reliable (p<0,05) rise of serotonin level and decrease of melatonin level. Thus, special features have direct influence on increase (p<0,05) of serotonin/melatonin index. Pregnant women of the main group have the index which was 2.5 time higher than the same index for women of the control group. Conclusions. Results of performed research among pregnant women have indicated that increase of state anxiety is accompanied by statistically reliable (p<0,05) rise of serotonin content in blood and statistically reliable (p<0,05) decrease of melatonin. Such results have direct influence on increase (p<0,05) of serotonin/melatonin index for pregnant women with medium and high levels of state anxiety comparing to the certain index for women with the low level of anxiety.

Responses of ajowan (Trachyspermum ammi L.) to exogenous salicylic acid and iron oxide nanoparticles under salt stress.

This research with a factorial arrangement was undertaken to investigate physiological responses of ajowan plants to foliar treatment of salicylic acid (1 mM) and nano-Fe2O3 (3 mM) under various salinity levels (0, 4, 8, 12 dS m-1 NaCl, respectively). Rising salinity enhanced sodium and endogenous SA contents, soluble sugars, protein, glycine betaine, proline, antioxidant enzymes activities, ROS generation, and lipid peroxidation, while reduced potassium and iron contents, membrane stability index, leaf water content, leaf pigments, root and shoot biomasses, and seed yield. Application of particularly SA and SA+nano-Fe2O3 alleviated salt toxicity via enhancing K+ uptake, K+/Na+ ratio, Fe content, endogenous level of SA, the activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and polyphenol oxidase), and most of the osmolytes. These changes were resulted in improving membrane stability index, leaf water content, leaf pigments, root and shoot growth, and finally seed yield of plants under moderate and severe salinities. Therefore, these treatments can additively enhance salt tolerance and physiological performance of ajowan through increasing antioxidant capacity, osmolytes, and photosynthetic pigments. Graphical Abstract .

Analysis of behavioral risks in adolescents with health limitations and with different anxiety levels

Aim. To assess peculiarities of lifestyle of adolescents with health limitations having diffe-rent levels of anxiety.
 Materials and Methods. In the research 89 adolescents with health limitations were involved studying at an educational institution of secondary professional education. The leading causes of disability (the main disease) were mental and behavioral disorders. Anxiety levels of the adolescents (State Trait Anxiety Inventory), the extent of nicotine addiction (Fagerstrom test) were studied, social factors, duration of night sleep were assessed, organization of leisure was analyzed.
 Results. Low state anxiety (SA, 30 points) was found in 76.2% of the examined adolescents, moderate SA (33-34 points) in 23.8%, 51.4% had a high level of trait anxiety (TA). A moderate negative correlation relationship was established between SA and TA of students (r=0.72, p=0.02). Assessment of the lifestyle of adolescents found the existence of the family disadvantages (each sixth student grew in a social institution); reduction in the night sleep duration (7 hours) in 30.8% of students; low physical activity (only 18.9% of students attended additional physical culture and sports classes). Nicotine addiction was found in more than 90% of students, 61.2% of students used strong drinks at least once in life. A correlation was established between living in a hostel (r=0.56, p=0.031), duration of night sleep (r=0.61, p=0.028), organization of passive rest (r=0.52, p=0.04) and the level of SA.
 Conclusion. The conducted research identified the following risk factors for justification of programs for prevention of development of desadaptation: factor of family disadvantages, bad habits, reduced duration of night sleep, low physical activity.

Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level

ABSTRACTSalicylic acid (SA) is an important signaling hormone in plant immunity. It can be synthesized by either the phenylpropanoid pathway or the isochorismate pathway, but mutant studies of this have been scarce in other species than Arabidopsis. Here we identified a mutation that introduced a stop-codon early in the barley gene for isochorismate synthase (ICS). We found that homozygous ics plants wilted if not sprayed with 1,4-dihydroxy-2-naphthoic acid, a precursor of phylloquinone, also synthesized via the isochorismate pathway. Interestingly, ics had unchanged SA, suggesting that the basal level of SA is synthesized via the phenylpropanoid pathway. Previous studies have failed seeing increased SA levels in barley after attack by the powdery mildew fungus, Blumeria graminis f.sp. hordei (Bgh), and indeed, we saw no changes in the interaction of ics with this fungus. Overall, we hope this mutant will be useful for other studies of SA in barley.

ESTIMATION OF PSYCHOEMOTIONAL STATE OF PREGNANT WOMEN WITH FETUS GROWTH INHIBITION

It is difficult to overestimate role of chronic psychoemotional stress in formation of fetus growth inhibition (FGI) at the effect of which, based on different stress resistance level, the signs of distress occur both for pregnant woman and fetus. In its turn, it causes the occurrence of disadaptation in mother-placenta-fetus system. The most favorable sign of this phenomenon is the disorder of fetus growth. Now the proved fact is that mother anxiety and birth of children with low weight are connected with each other.Purpose of research – determine role of psychoemotional disorders of pregnant women as source of risk of fetus growth inhibition based on complex clinical and psychological examination.Group of examined persons and research methods Complex clinical and psychological examination of 168 pregnant women with term of 28-33 weeks (screening) was performed. Complex of psychodiagnostic techniques aimed on research of psychological state of pregnant women had included the following: clinical interview, StateTrait Anxiety Inventory - STAI (C.D. Spielberger) adapted by Yu. L. Hanin and Eysenck Personality Inventory – EPQ (H.J. Eysenck) which estimates such psychic properties as neuropsychic lability, extraversion and psychoticism. Availability of fetus growth inhibition was estimated during delivery of baby with weigh-height parameters less than 10th percentile for this term of pregnancy. The main group is presented by 33 pregnant women with fetus growth inhibition and the control group comprising 135 pregnant women without signs of this complication.Results of research and their discussion Analysis of assessment results of level of SA occurring as emotional reaction on stress situation has permitted to determine statistically significant difference (р < 0.05) in research groups. Thus SA level average index in the main group made 35.1 ± 2.8 points and 32.6 ± 1.2 points in control group. During assessment of TA indicating anxiety as personal feature the statistically significant difference (р < 0.05) according to its level in research groups (43.2 ± 2.4 and 40.7 ± 1.3 points correspondingly) was not determined. Indices of neuroticism and psychoticism were statistically significantly (р < 0.05) higher in the main group.Conclusions. Results of performed research show the higher level of the state anxiety, neuroticism and psychoticism was determined statistically and significantly (р < 0.05) for women who gave birth to children with growth inhibition according to weight-height parameters. Connection of above mentioned indices is confirmed with availability of positive correlation of neuroticism with SA level (r = +0.202, p < 0.05) and, more significant, with TA level (r = +0.625, p < 0.05). Psychoemotional disorders of women during pregnancy should be considered as source of risk of fetus growth inhibition that, in its turn, indicates expediency for research of mechanisms of their mutual influence with stress- realizing and stress-limiting links of neurohumoral regulation of the present group of women.

Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts.

Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies have shown the beneficial effects of paracrine factors secreted from adult stem cells, such as exosomes, on skin aging. However, to date, few reports have demonstrated the use of exosomes derived from human pluripotent stem cells for the treatment of skin aging. In this study, we collected exosomes from the conditioned medium of human induced pluripotent stem cells (iPSCs) and investigated the effect on aged human dermal fibroblasts (HDFs). Cell proliferation and viability were determined by an MTT assay and cell migration capacity was shown by a scratch wound assay and a transwell migration assay. To induce photoaging and natural senescence, HDFs were irradiated by UVB (315 nm) and subcultured for over 30 passages, respectively. The expression level of certain mRNAs was evaluated by quantitative real-time PCR (qPCR). Senescence-associated-β-galactosidase (SA-β-Gal) activity was assessed as a marker of natural senescence. As a result, we found that exosomes derived from human iPSCs (iPSCs-Exo) stimulated the proliferation and migration of HDFs under normal conditions. Pretreatment with iPSCs-Exo inhibited the damages of HDFs and overexpression of matrix-degrading enzymes (MMP-1/3) caused by UVB irradiation. The iPSCs-Exo also increased the expression level of collagen type I in the photo-aged HDFs. In addition, we demonstrated that iPSCs-Exo significantly reduced the expression level of SA-β-Gal and MMP-1/3 and restored the collagen type I expression in senescent HDFs. Taken together, it is anticipated that these results suggest a therapeutic potential of iPSCs-Exo for the treatment of skin aging.

Level of biogenic amines in pregnant women with psycho-emotional disorders stipulated by anxiety

Object of research – to determine level of biogenic amines in pregnant women with psycho-emotional disorders stipulated by anxiety. Group of examined women and methods of research . 90 Pregnant women were examined in ІІ and ІІІ trimester of pregnancy. The main group includes 58 pregnant women with the medium and high level of state anxiety and 32 pregnant women with anxiety level of 30 points and less that indicates the low level of SA (control group). For estimation of the state anxiety and trait anxiety the scale of Spielberger-Hanin was used. Level of serotonin and melatonin was measured with fluorometric method in blood hemolysate of pregnant women. Results . According to the obtained results of biochemical research of hemolysate of packed red cells of blood of examined women it was stated that increase of state anxiety was accompanied by statistically reliable (p < 0.05) rise of serotonin level and decrease of melatonin level. Thus, special features have direct influence on increase (p < 0.05) of serotonin/melatonin index. Pregnant women of the main group have the index which was 2.5 times higher than the same index in women of the control group. Conclusions . Results of performed research among pregnant women have indicated that increase of state anxiety is accompanied by statistically reliable (p < 0.05) rise of serotonin content in blood and statistically reliable (p < 0.05) decrease of melatonin. Such results have direct influence on increase (p < 0.05) of serotonin/melatonin index in pregnant women with medium and high levels of state anxiety comparing to the certain index in women with the low level of anxiety.

Fusarium Infection Causes Phenolic Accumulations and Hormonal Disorders in Orobanche spp.

The physiological effects of Fusarium oxysporum on in-root parasitic weed, Orobanche spp. (broomrape) with references to change in plant hormones and secondary plant constituents were investigated. The levels of IAA, GA, ABA and JA in the experimental group were significantly lower than those in the control group, while the level of SA was higher in the experimental group. In secondary metabolic studies, the quantities of various phenols were measured in the two groups and catechin, syringic acid and p-coumaric acid amounts were significantly higher in the experimental group than in the control group, unlike gallic acid which have a lower amount. Consequently, in the light of all data, it was concluded that Fusarium oxysporum (1) causes heavy hormonal disorder, (2) triggered only SA-mediated defense and (3) induced intensively accumulation of phenolic substances in orobanche. Fusarium oxysporum causes lethal physiological damage on Orobanche spp.

Muscadinia rotundifolia 'Noble' defense response to Plasmopara viticola inoculation by inducing phytohormone-mediated stilbene accumulation.

Downy mildew (DM), one of the most devastating grape diseases worldwide, is caused by the biotrophic oomycete Plasmopara viticola (Pv). In general, grapevine responds to Pv infection with the accumulation of phytoalexins as part of the innate immune system, and diverse phytoalexins are induced on grapevines with different DM-resistance levels in response to Pv invasion. However, the regulation of phytoalexin biosynthesis during grapevine against Pv is still unclear. Herein, we detected stilbenes by UPLC-ESI-MS/MS and found that resveratrol was accumulated to higher level and earlier in the DM-immune Muscadinia rotundifolia 'Noble' than that in the DM-susceptible Vitis vinifera 'Thompson Seedless' after Pv inoculation. Additionally, a considerable amount of pterostilbene and ε-viniferin was found in 'Noble', while a little was detected in 'Thompson Seedless'. Resveratrol was glycosylated into piceid both in 'Noble' and 'Thompson Seedless' after Pv inoculation. The qPCR analysis of gene expression indicated that the resveratrol-synthesis gene (STS) was induced by Pv inoculation earlier in 'Noble' than that in 'Thompson Seedless', while the pterostilbene-synthesis gene (ROMT) was induced in 'Noble' but not in 'Thompson Seedless' at all. The piceid-synthesis gene (GT) was generally up-regulated in both cultivars. Sequence analysis of STS, ROMT, and GT promoters revealed that they contained cis-regulatory elements responsive to phytohormones and pathogens. Following Pv inoculation, the level of SA, MeJA, and ABA was found to be consistently higher in 'Noble' than those in 'Thompson Seedless'. The results of exogenous hormone elicitation further demonstrated that the accumulation of stilbenes was regulated by phytohormones. The earlier and higher accumulation of phytohormones and consequent induction of stilbene synthesis may play an important role in grapevine defense against downy mildew disease.