Plant Level Research Articles

Advances in sample preparation methods for pesticide residue analysis in medicinal plants: A focus on Nepal.

Medicinal plant safety is a rising challenge worldwide due to the continued overuse of pesticides to their maximum residue limits. Due to the high demand for medicinal plants, their production is being increased and sometimes protected by pesticide use. The analysis of these residues requires robust analytical methods to ensure the safety and quality of medicinal plants. Developing effective sample preparation for detecting pesticides is challenging, due to their diverse natures, classes, and physico-chemical characteristics. Hence, existing techniques and strategies are needed to improve the reliability of the results. The review discusses the current state of sample preparation techniques, analytical methods, and instrumental technologies employed in pesticide residue analysis in medicinal plants. It highlights the challenges, limitations, and advancements in the field, providing insights into the analytical strategies used to detect and quantify pesticide residues. Reliable, accessible, affordable, and high-resolution analytical procedures are essential to ensure that pesticide levels in medicinal plants are effectively regulated. By understanding the complexities of pesticide residue analysis in medicinal plants, this review article aims to support the conservation of medicinal plant resources, promote public health, and contribute to the development of sustainable strategies for ensuring the safety and quality of medicinal plants in Nepal. The findings of this review will benefit researchers, policymakers, and stakeholders involved in the conservation of medicinal plant resources and the promotion of public health.

IMPROVED DYNAMIC BOTANICAL AIR PURIFIER WITH LIGHT EFFECT FOR REDUCING CO2 CONCENTRATION IN CHAMBER

Aims: This study aimed to investigate the effectiveness of the DBAP in reducing CO2 levels and improving indoor air quality. It also explores the impact of adding blue light to enhance photosynthesis. Additionally, the study examines the ability of the DBAP's HEPA filter to reduce PM2.5 concentration. Methodology and results: The methodology used in this study involved testing the reduction of CO2 levels in plants using blue light as an enhancement. The researchers also developed a Dynamic Botanic Air Purifier (DBAP) that incorporated activated carbon and a fan as an active filter to improve CO2 reduction. The study measured the reduction level of CO2 in the plants with blue light enhancement, resulting in a 40% reduction. Subsequently, after adding the active filter to the plants, the CO2 reduction improved by an additional 11% compared to without the active filter. Additionally, the study found that the HEPA filter within the active filter effectively reduced the concentration of PM2.5 to 71.6%. Conclusion, significance and impact study: In conclusion, this study highlights the significance of addressing poor indoor air quality and its potential impact on occupant health and well-being. Developing the Dynamic Botanic Air Purifier (DBAP), incorporating plants, activated carbon, a fan, and blue light enhancement, offers a promising solution for improving indoor air quality and preventing Sick Building Syndrome (SBS). The findings of this study have the potential to inform the development of effective strategies and technologies aimed at creating healthier indoor environments, thus positively impacting public health and overall indoor air quality.

A review of changes at the phenotypic, physiological, biochemical, and molecular levels of plants due to high temperatures.

This review summarizes the physiological, biochemical, and molecular regulatory network changes in plants in response to high temperature. With the continuous rise in temperature, high temperature has become an important issue limiting global plant growth and development, affecting the phenotype and physiological and biochemical processes of plants and seriously restricting crop yield and tree growth speed. As sessile organisms, plants inevitably encounter high temperatures and improve their heat tolerance by activating molecular networks related to heat stress, such as signal transduction, synthesis of metabolites, and gene expression. Heat tolerance is a polygenic trait regulated by a variety of genes, transcription factors, proteins, and metabolites. Therefore, this review summarizes the changes in physiological, biochemical and molecular regulatory networks in plants under high-temperature conditions to lay a foundation for an in-depth understanding of the mechanisms involved in plant heat tolerance responses.

Trade‐offs in rooting strategy dimensions along an edaphic gradient in a grassland ecosystem

Abstract Roots are essential to the diversity and functioning of plant communities, but trade‐offs in rooting strategies are still poorly understood. We evaluated existing frameworks of rooting strategy trade‐offs and tested their underlying assumptions, guided by the hypothesis that community‐level rooting strategies are best described by a combination of variation in organ‐level traits, plant‐level root:shoot allocation and symbiosis‐level mycorrhizal dependency. We tested this hypothesis using data on plant community structure, above‐ and below‐ground biomass, eight root traits including mycorrhizal colonisation and soil properties from an edaphic gradient driven by elevation and water availability in sandhills prairie, Nebraska, USA. We found multidimensional trade‐offs in rooting strategies represented by a two‐way productivity‐durability trade‐off axis (captured by root length density and root dry matter content) and a three‐way resource acquisition trade‐off between specific root length, root:shoot mass ratio and mycorrhizal dependence. Variation in rooting strategies was driven to similar extents by interspecific differences and intraspecific responses to soil properties. Organ‐level traits alone were insufficient to capture community‐level trade‐offs in rooting strategies across the edaphic gradient. Instead, trait variation encompassing organ, plant and symbiosis levels revealed that consideration of whole‐plant phenotypic integration is essential to defining multidimensional trade‐offs shaping the functional variation of root systems. Read the free Plain Language Summary for this article on the Journal blog.

An Implantable and Self-Powered Sensing System for the In Vivo Monitoring of Dynamic H2O2 Level in Plants

The real-time monitoring of hydrogen peroxide (H2O2) is significant for understanding the working mechanism of signal molecules, breeding for stress tolerance, and diagnosing plant health. However, it remains a challenge to realize real-time monitoring of the dynamic H2O2 level in plants. Here, we report an implantable and self-powered sensing system for the continuous monitoring of H2O2 level in plants. A photovoltaic (PV) module is integrated into a sensing system to collect sunlight or artificial light in the planting environment in order to continuously power an implantable microsensor. The transmission process of the H2O2 signal was monitored and analyzed in vivo, and the time and concentration specificity of the H2O2 signal for abiotic stress were resolved. This implantable system provides a promising analysis tool for key signal molecules in plants and might be extended to the real-time monitoring of signaling molecules in other crops.

A normalization method that controls for total RNA abundance affects the identification of differentially expressed genes, revealing bias toward morning-expressed responses.

RNA-Sequencing is widely used to investigate changes in gene expression at the transcription level in plants. Most plant RNA-Seq analysis pipelines base the normalization approaches on the assumption that total transcript levels do not vary between samples. However, this assumption has not been demonstrated. In fact, many common experimental treatments and genetic alterations affect transcription efficiency or RNA stability, resulting in unequal transcript abundance. The addition of synthetic RNA controls is a simple correction that controls for variation in total mRNA levels. However, adding spike-ins appropriately is challenging with complex plant tissue, and carefully considering how they are added is essential to their successful use. We demonstrate that adding external RNA spike-ins as a normalization control produces differences in RNA-Seq analysis compared to traditional normalization methods, even between two times of day in untreated plants. We illustrate the use of RNA spike-ins with 3' RNA-Seq and present a normalization pipeline that accounts for differences in total transcriptional levels. We evaluate the effect of normalization methods on identifying differentially expressed genes in the context of identifying the effect of the time of day on gene expression and response to chilling stress in sorghum.

Elevated tolerance of both short-term and continuous drought stress during reproductive stages by exogenous application of hydrogen peroxide on soybean

The global production of soybean, among other drought-susceptible crops, is reportedly affected by drought periods, putting more pressure on food production worldwide. Drought alters plants’ morphology, physiology and biochemistry. As a response to drought, reactive oxygen species (ROS) concentrations are elevated, causing cellular damage. However, lower concentrations of ROS were reported to have an alleviating role through up-regulating various defensive mechanisms on different levels in drought-stressed plants. This experiment was set up in a controlled environment to monitor the effects of exogenous spray of different (0, 1, 5 and 10 mM) concentrations of H2O2 on two soybean genotypes, i.e., Speeda (drought-tolerant), and Coraline (drought-susceptible) under severe drought stress conditions (induced by polyethylene glycol) during flowering stage. Furthermore, each treatment was further divided into two groups, the first group was kept under drought, whereas drought was terminated in the second group at the end of the flowering stage, and the plants were allowed to recover. After 3 days of application, drought stress significantly decreased chlorophyll-a and chlorophyll-b, total carotenoids, stomatal conductance, both optimal and actual photochemical efficiency of PSII (Fv/Fm and Df/Fm, respectively), relative water content, specific leaf area, shoot length and dry weight, and pod number and fresh weight, but significantly increased the leaf concentration of both proline and total soluble sugars, the root length, volume and dry weight of both genotypes. The foliar application of 1 mM and 5 mM H2O2 on Speeda and Coraline, respectively enhanced most of the decreased traits measurably, whereas the 10 mM concentration did not. The group of treatments where drought was maintained after flowering failed to produce pods, regardless of H2O2 application and concentration, and gradually deteriorated and died 16 and 19 days after drought application on Coraline and Speeda, respectively. Overall, Speeda showed better performance under drought conditions. Low concentrations of foliar H2O2 could help the experimented soybean genotypes better overcome the influence of severe drought during even sensitive stages, such as flowering. Furthermore, our findings suggest that chlorophyll fluorescence and the cellular content of proline and soluble sugars in the leaves can provide clear information on the influence of both drought imposition and H2O2 application on soybean plants.

Bacillus subtilis NBRI-W9 simultaneously activates SAR and ISR against Fusarium chlamydosporum NBRI-FOL7 to increase wilt resistance in tomato.

The study aimed to determine the pathogenicity of Fusarium species currently prevalent in tomato fields having history of chemical fungicide applications and determine the bio-efficacy of Bacillus subtilis NBRI-W9 as a potent biological control agent. Fusarium was isolated from surface-sterilized infected tomato plants collected from fields. Pathogenicity of 30 Fusarium isolates was determined by in vitro and in vivo assays. Following Koch's postulates, F. chlamydosporum (FOL7) was identified as a virulent pathogen. The biological control of FOL 7 by B. subtilis NBRI-W9 (W9) and the colonization potential of W9 were established using spontaneous rifampicin-resistant mutants. W9 showed 82% inhibition of FOL7 on a dual-culture plate and colonization levels in tomato plants of ∼5.5, ∼3.3, and ∼2.2 log10 CFU/g in root, stem, and leaf tissue, respectively. Antagonistic activity was shown by scanning electron microscopy (SEM) and cell-wall-degradative enzymes. W9 reduced FOL7 infection in net-house and field experiments by 60% and 41%, respectively. Biochemical investigation, defence enzymes, defence gene expression analysis, SEM, and field studies provide evidence of hyperparasitism and induced resistance as the mode of biological control. The study also demonstrates that the potent biocontrol agent W9, isolated from Piper, can colonize tomato plants, control fungal disease by inducing induced systemic resistance (ISR) and systemic acquired resistance (SAR) simultaneously, and increase crop yield by 21.58% under field conditions. This study concludes that F. chlamydosporum (NBRI-FOL7) is a potent, fungicide-resistant pathogen causing wilt in tomatoes. NBRI-W9 controlled FOL7 through mycoparasitism and simultaneously activated ISR and SAR in plants, providing an attractive tool for disease control that acts at multiple levels.

Simulations of a possible configuration of Premium Power Park

The voltage dip and the sudden electrical energy interruption are, probably, the most important disturbances of the power quality in the electrical system. Several technical solutions exist to mitigate voltage dips, respectively at plant or system level as for example: • the installation of Custom Power (CUSPO) for a single customer; • the Premium Power Park (PPP) for a group of customers. According to the PPP concept, the tenants of an industrial or commercial office park would be provided with a guaranteed level of electrical service quality made possible by Custom Power devices, such as the Dynamic Voltage Restorer (DVR), the Distribution Static Compensator (DSTATCOM – Distribution STATCOM), the medium voltage Static Transfer Switch (STS). The paper summarizes the Premium Power Park idea and a possible configuration of it, studied by ERSE, including the system aspects and the analysis of the combined operation or interaction of the Custom Power.

MicroRNA-encoded regulatory peptides modulate cadmium tolerance and accumulation in rice.

MicroRNAs (miRNAs)are small noncoding RNAs that play a vital role in plant responses to abiotic and biotic stresses. Recently, it has been discovered that some primary miRNAs (pri-miRNAs)encode regulatory short peptides called miPEPs. However, the presence of miPEPs in rice, and their functions in response to abiotic stresses, particularly stress induced by heavy metals, remain poorly understood. Here, we identified a functional small peptide (miPEP156e)encoded by pri-miR156e that regulates the expression of miR156 and its target SPL genes, thereby affecting miR156-mediated cadmium (Cd) tolerance in rice. Overexpression of miPEP156e led to decreased uptake and accumulation of Cd and reactive oxygen species (ROS)levels in plants under Cd stress, resulting in improved rice Cd tolerance, as observed in miR156-overexpressing lines. Conversely, miPEP156e mutants displayed sensitivity to Cd stress due to the elevated accumulation of Cd and ROS. Transcriptome analysis further revealed that miPEP156e improved rice Cd tolerance by modulating Cd transporter genes and ROS scavenging genes. Our study provides insights into the regulatory mechanism of miPEP156e in rice response to Cd stress and demonstrates the potential of miPEPs as an effective tool for improving crop abiotic stress tolerance.

LeTra: a leaf tracking workflow based on convolutional neural networks and intersection over union

BackgroundThe study of plant photosynthesis is essential for productivity and yield. Thanks to the development of high-throughput phenotyping (HTP) facilities, based on chlorophyll fluorescence imaging, photosynthetic traits can be measured in a reliable, reproducible and efficient manner. In most state-of-the-art HTP platforms, these traits are automatedly analyzed at individual plant level, but information at leaf level is often restricted by the use of manual annotation. Automated leaf tracking over time is therefore highly desired. Methods for tracking individual leaves are still uncommon, convoluted, or require large datasets. Hence, applications and libraries with different techniques are required. New phenotyping platforms are initiated now more frequently than ever; however, the application of advanced computer vision techniques, such as convolutional neural networks, is still growing at a slow pace. Here, we provide a method for leaf segmentation and tracking through the fine-tuning of Mask R-CNN and intersection over union as a solution for leaf tracking on top-down images of plants. We also provide datasets and code for training and testing on both detection and tracking of individual leaves, aiming to stimulate the community to expand the current methodologies on this topic.ResultsWe tested the results for detection and segmentation on 523 Arabidopsis thaliana leaves at three different stages of development from which we obtained a mean F-score of 0.956 on detection and 0.844 on segmentation overlap through the intersection over union (IoU). On the tracking side, we tested nine different plants with 191 leaves. A total of 161 leaves were tracked without issues, accounting to a total of 84.29% correct tracking, and a Higher Order Tracking Accuracy (HOTA) of 0.846. In our case study, leaf age and leaf order influenced photosynthetic capacity and photosynthetic response to light treatments. Leaf-dependent photosynthesis varies according to the genetic background.ConclusionThe method provided is robust for leaf tracking on top-down images. Although one of the strong components of the method is the low requirement in training data to achieve a good base result (based on fine-tuning), most of the tracking issues found could be solved by expanding the training dataset for the Mask R-CNN model.

Osmotic-induced cleaning is effective in long-term fouling mitigation of reverse osmosis elements at pilot plant level

Osmotic backwashing (OBW), a promising environment-friendly membrane cleaning technique, has been mostly studied at the lab scale. In this work, we investigated the efficacy of periodic OBW in fouling mitigation at a pilot-plant level. After initial optimization of the major OBW parameters, it was applied periodically to a single-element RO module used for seawater desalination. The continuous monitoring of major performance parameters and comparison to a control unit revealed the success of periodic OBW in fouling mitigation: significantly lower feed channel pressure (FCP) drop (50 – 100 kPa vs. 200 kPa), less decline in normalized permeate flow (10 % vs. 30 %), and virtually unaffected salt rejection (99.5% vs. 99.2%). Findings from foulant analyses and membrane autopsy correlated well with the filtration results: fouling layer on the backwashed membrane was less dense and had a lower inorganic content (~ 57%) compared to the control (~ 72%), and two important properties, the surface roughness (~ 160 vs. 270 nm) and zeta potential (18 vs. 10 mV) witnessed a smaller increase. In summary, OBW was quite effective in mitigating fouling and its effects on membrane performance.

Regulation of nitric oxide by phytoglobins in Lotus japonicus is involved in mycorrhizal symbiosis with Rhizophagus irregularis

Various reactive molecular species are generated in plant–microbe interactions, and these species participate in defense and symbiotic responses. Leguminous plants successfully establish symbiosis by maintaining an appropriate level of nitric oxide (NO), which is generated in the roots and nodules during root nodule symbiosis. Phytoglobin (plant hemoglobin) controls NO levels in plants. In this study, we investigated mycorrhizal symbiosis, which occurs in more than 80% of land plants, between Rhizophagus irregularis and Lotus japonicus to clarify the involvement of phytoglobin-mediated NO regulation. The mycorrhizae of L. japonicus exhibited higher NO levels in the presence of R. irregularis than in its absence, especially at the infection site. LjGlb1–1, a phytoglobin that regulates NO level in L. japonicus, was upregulated during symbiosis with R. irregularis. In transformed hairy roots carrying the ProLjGlb1–1:GUS construct, LjGlb1–1 expression was observed at the R. irregularis infection site. We further examined the symbiotic phenotypes of L. japonicus lines with high and low LjGlb1–1 expression with R. irregularis. During mycorrhizal symbiosis, the high LjGlb1–1 expression line exhibited better growth than the wild-type, whereas the low expression line exhibited poor growth. In addition, the expression of LjPT4, a phosphate transporter specific to mycorrhizal symbiosis, was higher in the high LjGlb1–1 expression line, whereas that of the tubulin gene of R. irregularis was lower in the low LjGlb1–1 expression line than in the wild-type. These results confirm that NO regulation by LjGlb1–1 is involved in mycorrhizal symbiosis in L. japonicus, as it is reportedly in nitrogen-fixing symbiosis.

CsCuAO1 Associated with CsAMADH1 Confers Drought Tolerance by Modulating GABA Levels in Tea Plants.

Our previous study showed that COPPER-CONTAINING AMINE OXIDASE (CuAO) and AMINOALDEHYDE DEHYDROGENASE (AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea through the polyamine degradation pathway. However, their biological function in drought tolerance has not been determined. In this study, Camellia sinensis (Cs) CsCuAO1 associated with CsAMADH1 conferred drought tolerance, which modulated GABA levels in tea plants. The results showed that exogenous GABA spraying effectively alleviated the drought-induced physical damage. Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine by stimulating reactive oxygen species' scavenging capacity and stomatal movement. However, the suppression of CsCuAO1 or CsAMADH1 in tea plants resulted in increased sensitivity to drought treatment. Moreover, co-overexpressing plants increased GABA accumulation both in an Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter gene, CsGAT1, was identified, whose expression was strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in the response to drought stress through a dynamic GABA-putrescine balance. Our data will contribute to the characterization of GABA's biological functions in response to environmental stresses in plants.

Cellular dynamics in the maize leaf growth zone during recovery from chilling depends on the leaf developmental stage.

A novel non-steady-state kinematic analysis shows differences in cell division and expansion determining a better recovery from a 3-day cold spell in emerged compared to non-emerged maize leaves. Zea mays is highly sensitive to chilling which frequently occurs during its seedling stage. Although the direct effect of chilling is well studied, the mechanisms determining the subsequent recovery are still unknown. Our goal is to determine the cellular basis of the leaf growth response to chilling and during recovery of leaves exposed before or after their emergence. We first studied the effect of a 3-day cold spell on leaf growth at the plant level. Then, we performed a kinematic analysis to analyse the dynamics of cell division and elongation during recovery of the 4th leaf after exposure to cold before or after emergence. Our results demonstrated cold more strongly reduced the final length of non-emerged than emerged leaves (-13 vs. -18%). This was not related to growth differences during cold, but a faster and more complete recovery of the growth of emerged leaves. This difference was due to a higher cell division rate on the 1st and a higher cell elongation rate on the 2nd day of recovery, respectively. The dynamics of cell division and expansion during recovery determines developmental stage-specific differences in cold tolerance of maize leaves.

The implementation of the SPAD-502 Chlorophyll meter for the quantification of nitrogen content in Arabica coffee leaves

The utilization of a non-destructive SPAD-502 chlorophyll meter, which enables the measurement of nitrogen status in plant leaves, has gained popularity in agronomic crops. Its application to horticultural crops like coffee remains relatively uncommon. The device provides quick and real-time measurements, helping to provide on-time nitrogen fertilizer to coffee plants before deficiency signs occur. Coffee leaves are characterized by thick and waxy leaves, together with many layers of tree crown. Therefore, the objective of this study was to develop a method for measuring nitrogen levels in coffee plants using the SPAD-502 Chlorophyll meter for an appropriate nitrogen fertilizer application rate in Arabica coffee plants.•Coffee trees were separated into upper, middle and lower levels. Data on SPAD values and total nitrogen were analyzed.•Pearson Correlation Coefficient (R), Coefficient of Determination (R2) and linear regression were calculated for different three levels of both SPAD-502 and total nitrogen values.•The results revealed a strong correlation (R2 = 0.63) between the SPAD readings of coffee leaves obtained from the upper canopy and their nitrogen content.These findings can provide a good concept of which coffee crown level will be a better part for measuring N content using a SPAD-502 Chlorophyll meter.

Transcription factor MYB8 regulates iron deficiency stress response in Arabidopsis

Iron (Fe) is a crucial microelement for humans, animals, and plants. Insufficient Fe levels in plants impede growth and diminish photosynthesis, thus decreasing crop production. Notably, approximately one-third of the soil worldwide is alkaline and prone to Fe deficiency. Therefore, understanding the mechanisms underlying Fe absorption and transportation in plants can enhance Fe bioavailability in crops. In this study, the role of the transcription factor MYB8 in plant response to Fe deficiency in Arabidopsis was investigated via reverse genetics. Phenotype analysis revealed that the functional deletion mutant of MYB8 gene exhibited sensitivity to Fe deficiency stress, as indicated by shorter root length, lower chlorophyll content, and Fe concentration. Conversely, MYB8 overexpression strain showed a tolerant phenotype. Furthermore, qRT-PCR identified possible downstream MYB8-regulated genes. Moreover, MYB8 regulated the expression of iron-regulated transporter 1 (IRT1) by binding to the MYB binding sites motif (‘AACAAAC’) in its promoter.

Population Dynamic of Fruit Fly Pests Bactrocera spp. in Salacca Orchard in Relation to Host Plants and Climate Factors

Fruit flies are an economic important of pest on horticultural crops. One of the commodities that are affected much due to fruit fly attack is salacca var. pondoh. The number of fruit flies attacking salacca increased from 2018 to 2021. The aims of the research was to study the population dynamics in salacca orchards in relation to climate factors and host plants. The research was conducted from July 2022 to May 2023 in salacca orchards that are members of the Salacca Farmers Association Mitra Turindo in Wonokerto, Turi, Sleman, Yogyakarta, Indonesia. The methods were counting the number of fruit flies trapped in three observation groups with different levels of sanitation and host plants around salacca orchards. Descriptive and arithmetic methods were used to determine the pattern of population oscillation among the three observation groups and differences in sanitation levels and host plants around the salacca orchards. The result showed that population fluctuations of <em>Bactrocera</em> spp. in salacca orchards were correlated with rainfall and relative humidity and less correlated with temperature and wind speed. Fruit fly abundance was lower in salacca orchards with better sanitation and fewer host plants around the salacca orchard.

Quantifying the Impact of Street Greening during Full-Leaf Seasons on Emotional Perception: Guidelines for Resident Well-Being

Quantifying the emotional impact of street greening during the full-leaf seasons in spring, summer, and fall is important for well-being-focused urban construction. Current emotional perception models usually focus on the influence of objects identified through semantic segmentation of street view images and lack explanation. Therefore, interpretability models that quantify street greening’s emotional effects are needed. This study aims to measure and explain the influence of street greening on emotions to help urban planners make decisions. This would improve the living environment, foster positive emotions, and help residents recover from negative emotions. In Hangzhou, China, we used the Baidu Map API to obtain street view images when plants were in the full-leaf state. Semantic segmentation was used to separate plant parts from street view images, enabling the calculation of the Green View Index, Plant Level Diversity, Plant Color Richness, and Tree–Sky View Factor. We created a dataset specifically designed for the purpose of emotional perception, including four distinct categories: pleasure, relaxation, boredom, and anxiety. This dataset was generated through a combination of machine learning algorithms and human evaluation. Scores range from 1 to 5, with higher values indicating stronger emotions and lower values indicating less intense ones. The random forest model and Shapley Additive Explanation (SHAP) algorithm were employed to identify the key indicators that affect emotions. Emotions were most affected by the Plant Level Diversity and Green View Index. These indicators and emotions have an intricate non-linear relationship. Specifically, a higher Green View Index (often indicating the presence of 20–35 fully grown trees within a 200 m range in street view images) and a greater Plant Level Diversity significantly promoted positive emotional responses. Our study provided local planning departments with support for well-being-focused urban planning and renewal decisions. Based on our research, we recommend the following actions: (1) increase the amount of visible green in areas with a low Green View Index; (2) plant seasonal and flowering plants like camellia, ginkgo, and goldenrain trees to enhance the diversity and colors; (3) trim plants in areas with low safety perception to improve visibility; (4) introduce evergreen plants like cinnamomum camphor, osmanthus, and pine.

Exogenous priming of chitosan induces resistance in Chinese prickly ash against stem canker caused by Fusarium zanthoxyli

Stem canker is a highly destructive disease that threatens prickly ash plantations in China. This study demonstrated the effective control of stem canker in prickly ash using chitosan priming, reducing lesion areas by 46.77 % to 75.13 % across all chitosan treatments. The mechanisms underlying chitosan-induced systemic acquired resistance (SAR) in prickly ash were further investigated. Chitosan increased H2O2 levels and enhanced peroxidase and catalase enzyme activities. A well-constructed regulatory network depicting the genes involved in the SAR and their corresponding expression levels in prickly ash plants primed with chitosan was established based on transcriptomic analysis. Additionally, 224 ZbWRKYs were identified based on the whole genome of prickly ash, and their phylogenetic evolution, conserved motifs, domains and expression patterns of ZbWRKYs were comprehensively illustrated. The expression of 12 key genes related to the SAR was significantly increased by chitosan, as determined using reverse transcription-quantitative polymerase chain reaction. Furthermore, the activities of defensive enzymes and the accumulation of lignin and flavonoids in prickly ash were significantly enhanced by chitosan treatment. Taken together, this study provides valuable insights into the chitosan-mediated activation of the immune system in prickly ash, offering a promising eco-friendly approach for forest stem canker control.