Tunisia’s northwestern region offers a rich and diverse civilization heritage and cultural potential. However, it has been socially and economically marginalised since the 70s. This study explores the link between urban cultural heritage and the construction of collective identity, emphasising the potential of built heritage to foster sustainable community-based development. In addition to physical attributes, the literature highlights the importance of social interactions in shaping territorial identity. Identity, in this context, is not static but a dynamic territorial construction that integrates architectural, urban, and social dimensions. The cities of El Kef and Tabarka serve as case studies of spatially and culturally marginalised areas, facing significant challenges to both tangible and intangible heritage. With a negative population growth rate (−0.36% between 2004 and 2014) and the lowest economic development indicator nationally (0.3% in 2012), these cities reflect the urgent need for an alternative approach. Through spatial diagnosis, interviews, and stakeholder engagement, the research demonstrates that a renewed territorial model—grounded in heritage valorization and local identity—can support inclusive and adaptive development. Key findings reveal a generational gap in the perception and representation of heritage between younger and older residents. This indicates that cultural identity is not a static inheritance but a dynamic process requiring active community investment. Ultimately, the study concludes that urban identity assets critically influence the capacity of a community to build a shared vision for the enhancement of its territorial identity. This reconnection between territory, memory, and planning enables a collective reappropriation of space, proposing a long-term vision for heritage-integrated urban regeneration.

This paper reports the first discovery in Tunisia of Orobanche ballotae (Orobanchaceae) parasiting on Pseudodictamnus hirsutus, its specific plant-host, within the mountains of Le Kef region in the northwestern part of the country. The species was considered to be endemic to Spain but reported later also from Greece and doubtfully from Morocco. The population reported confirms it as a floristic record to the African holoparasitic flora. Moreover, its geographical distribution together with its phenology, habitat and ecology in Tunisia are presented.

High-resolution Danian–Selandian benthic foraminifera-based paleoenvironmental reconstruction and response to sequence stratigraphy: Inferences from the Elles section, El Kef Basin, Tunisia

The Campanian-Maastrichtian benthic foraminiferal assemblages at the Elles section (Tunisia): A perspective for paleoenvironmental, paleobathymetric and sea-level fluctuation reconstruction

IntroductionKnowledge of the risk factors of bullying victimization in adolescents is crucial for the implementation of preventive measures. This study aimed to determine the prevalence and associated factors of bullying victimization and to identify its correlation with mental health outcomes among middle school students in Tunisia.MethodsA cross-sectional study was conducted using a multi-stage cluster sampling technique to recruit a sample of 1111 students from 10 middle schools in El kef (Tunisia). The revised Olweus Bully/Victim Questionnaire was used to assess the prevalence and types of bullying victimization and the perceived efforts of others to counteract bullying. The Strengths and Difficulties Questionnaire (SDQ) was used for screening emotional and behavioral problems. Multivariate logistic regression analysis was conducted to determine associated factors of bullying victimization. Additionally, we tested whether emotional and behavioral problems were present for bullying victims.ResultsThe findings reported that 45.8% (95%CI = 45.5–46.0), of the total number of participants experienced school bullying victimization. Multivariate logistic regression analysis, revealed that repeating a grade (OR = 1.82, 95%CI = 1.31–2.54), having a working father (OR = 17.68; 95%CI = 2.29–136,15), and having a working mother (OR = 1.88, 95%CI = 1.39–2.53) were the factors significantly associated with bullying victimization. Nevertheless, a higher mother’s educational level (OR = 0.76, 95%CI = 0.67–0.88) was a protective factor against bullying victimization. The self-reported SDQ revealed that the total difficulties score was significantly higher among victims (17.46 ± 5.30 vs. 20.86 ± 5.06, p<0.01).ConclusionsThis study showed that the prevalence of bullying in middle schools was high and it significantly led to mental health problems. National policies for bullying prevention within schools are potentially needed. Improving students’ problem-solving and soft skills is also essential.

“X-ray diffraction (XRD)” rather than “X-ray fluorescence (XRF)” was printed in the captions to Figure 7, Figure 9, and Figure 10. See PDF file for details.

Iron age genomic data from Althiburos – Tunisia renew the debate on the origins of African taurine cattle

Abstract Potato ( Solanum tuberosum L.) is a staple food widely consumed in Tunisia and a source of nutrients beneficial for human health. The tubers are, indeed, important source of vitamins, minerals, and bioactive compounds, including carotenoids, polyphenols, and flavonoids. However, data regarding the functional quality of potato varieties grown and consumed in Tunisia are very scarce. For this reason, two field trials were conducted to focus on the effect of the growing season on the functional quality of four potato genotypes (Selena, El Beidha, Naima, and CL07F172.5): a first autumnal trial in Teboulba, Monastir (September 2015–February 2016), which is the late cropping season (LCS) and a second summer cropping season (SCS) in El ksour, El Kef region at an altitude &gt; 1000 m (July 2015–October 2015). Total carotenoids, total phenolics, and flavonoids contents, as well as the hydrophilic radical scavenging activity and lipophilic radical scavenging activity (LRSA) were determined. The obtained results confirmed the importance of potato tubers as a source of bioactive compounds. All quality traits varied significantly among varieties and cropping seasons. Tubers grown under colder weather conditions (LCS: minimum and maximum averages temperatures and relative humidity (RH) ranging between 14.25 and 22.55°C and 25.46% and 83.07%, respectively) accumulated the highest total vitamin C content and exhibited the highest LRSA values. However, temperature and warmer growing conditions (SCS: minimum and maximum averages temperatures and RH ranging between 19.74 and 34.22°C and 35.46% and 87.51%, respectively) probably enhanced the synthesis and accumulation of total carotenoids, total phenolics, and total flavonoids, associated with higher LRSA values.

The Cretaceous/Paleogene (K/Pg) boundary is marked by one of the largest mass extinctions in Earth’s history, with geological evidence for this event being expressed in hundreds of locations worldwide. An extensively studied section located near El Kef, northwestern Tunisia, is characterized by the classic iridium-rich K/Pg boundary layer, abundant and well-preserved microfossils, and apparently continuous sedimentation throughout the early Danian with no previously described structural complication. These features led to its designation in 1991 as the Global Stratigraphic Section and Point (GSSP) for the base of the Danian (i.e., the K/Pg boundary). However, the outcrop section has become weathered, and the “golden spike” marking the GSSP is difficult to locate. Therefore, the El Kef Coring Project aimed to provide a continuous record of unweathered sediments across the K/Pg transition in cores recovered from five rotary-drilled holes located close to the El Kef GSSP. Here, we present new, high-resolution lithologic, biostratigraphic, and geochemical data from these cores. The recovered stratigraphic successions of each hole (all drilled within ∼75 m of one another) are unexpectedly different, and we identified a formerly unknown unconformity within planktic foraminiferal biozone P1b. Our results provide evidence that sedimentation at El Kef was not as continuous or free from structural complication as previously thought. Despite these challenges, we present a new composite section from the five El Kef holes and an age model correlated to the orbitally tuned record at Walvis Ridge, South Atlantic Ocean, which is critical in placing the paleoenvironmental and paleoecological records from El Kef in a global context.

Abstract Background and aim Bullying is a complex and widespread public health issue that one can be exposed to at any age and at any field, but it is considered particularly frequent during times of transition in children’s and adolescents’ lives. This study aims to investigate the prevalence and various forms of bullying victimization and explore culture of bullying victimization among students. Methods A cross-sectional study was conducted during the Academic year 2018/2019 among middle school students in the governorate of El Kef, Tunisia. The sampling method adopted for this research was a cluster sampling technique. Data were collected using self-administered questionnaires. The Students’ involvement in bullying victimization was assessed using a validated Arabic version of the revised Olweus Bully/Victim Questionnaire. Results A total of 1111 middle school students were enrolled. The prevalence of bullying victimization behavior in this study was 45.8 % [95% CI: 45.5- 46]. Looking at the forms of bullying experienced by students: “Being called mean names” was the most prevalent form of being bullied with 26.9% (n = 299), followed by 16.9% reported “being kicked in some place”, 16.3% reported “being bullied through false rumors” and 14.3% experienced bullying through messages, calls or images by means of mobile phones or Internet and 8.1% of the students reported being bullied with sexual gestures or comments. Verbal bullying (29.5%) was the most common type of victimization, followed by physical victimization, relational victimization and cyber victimization with 22.5%, 22.2% and 14.3% respectively. More than half of the students (58.5%) were more likely to inform others about incidents of bullying in their schools, mainly their parents (36.6%) or a friend (32.6%). Conclusions Bullying is serious and major public health issue that have a negative impact on adolescents’ well-being, and require special attention at the family, school, and community level Key messages • High prevalence of bullying victimization with predominace of verbal type. • Culture of bullying victimization and taking care of victims is still weak.

<p indent="0mm">The Cretaceous-Paleogene (K-Pg) mass extinction that ended the age of the dinosaurs occurred 66 million years ago, with competing hypotheses including the Deccan Traps volcanic eruption, climate change, and sea level change. However, it is the Chicxulub asteroid impact that has been widely accepted as the most plausible cause. Whatever the reason, the Earth’s ecological environment changed dramatically, leading to a mass extinction of both terrestrial and marine life. Primary producers lie at the base of the food chain and support higher trophic levels, so play an important role in the marine ecosystem. The predominant primary producers in the ocean, coccolithophores (phytoplankton with small CaCO₃ scales covering their cell surface), were among those most severely devastated by the K-Pg mass extinction. So, studying fossil coccolithophores across the K-Pg boundary can provide a better understanding of the destruction and post-event recovery processes of marine primary production (PP) and export production (EP), and the changes and controlling mechanisms of the marine biological carbon pump in the current global climate change. The four hypotheses previously proposed to interpret the post-event primary production, namely strangelove ocean (collapsed PP), living ocean (collapsed EP), resilient ocean (continued PP and EP), and heterogeneous ocean (geography-dependent responses of PP and EP), are illustrated and compared, and their pros and cons reviewed. A dataset is compiled from multiple locations in the global ocean basin including Deep Sea Drilling Program Site 465, Ocean Drilling Program Sites 690, 1210, and 1262, and the outcrop at El Kef (Tunisia), with each location having a high-precision cyclostratigraphic time control and an ocean surface-to-deep (bulk carbonate-benthic foraminifera) <italic>δ</italic>¹³C vertical gradient. This global dataset is analyzed to explore the changes in the efficiency of global marine biological carbon pump after the K-Pg boundary event. Three important patterns are observed: (1) Prior to the K-Pg event, the vertical <italic>δ</italic>¹³C gradient in all ocean basins falls between 0.8‰–1.5‰ and is consistent with that in the modern ocean, and this gradient seems to gradually increase from the shelf to the deep ocean possibly due to higher efficiency of the biological carbon pump in the open ocean commonly characterized by oligotrophy; (2) the global ocean vertical <italic>δ</italic>¹³C gradient collapses after the K-Pg event, suggesting drastic changes in primary and/or export production in the euphotic zone; (3) the vertical <italic>δ</italic>¹³C gradient collapse results from the abrupt negative shift of the surface ocean <italic>δ</italic>¹³C, and the magnitude of this negative shift gradually decreases from the continental shelf to the deep sea, which may be related to the selective extinction of shallow-water grazers, swimming organisms and plankton, as well as the limited buffering capacity of shelf environments. After the K-Pg event, the main primary producers change from larger coccolithophores to smaller picoplankton, and the more recalcitrant dissolved organic carbon is able to sink to the seafloor due to the operation of the microbial carbon pump in the photic zone, leading to sustained efficiency of the biological carbon pump. Although the picoplankton-dominated biological carbon pump is not as efficient as that by coccolithophores, it is still capable of removing enough nutrients and harmful substances from the photic zone via long-term operation, not only providing food for higher trophic level organisms, but improving the marine physicochemical environment that deteriorated after the K-Pg asteroid impact. This process paves the way for the restoration and development of the entire marine ecosystem. The switch of key marine primary producers and the controlling factors involved are critical scientific issues that deserve more focus in future studies of the deep-time marine carbon cycle. The regional or global variation patterns in the biological carbon pump after the K-Pg event reveal how different marine ecosystems operate under extreme environmental stress, and provide a reference for better understanding and predicting how they respond to the ongoing rapid global climate change.

A historical review of the extinction, survival, and evolutionary models of planktic foraminifera proposed for the Cretaceous/Paleogene boundary (KPB) mass extinction event sometimes leaves the impression that there is still no conclusive evidence to support any single one of them. Two main models have been put forward: i) catastrophic mass extinction, almost total for some authors, compatible with the geologically instantaneous paleoenvironmental effects of a large meteorite impact (Chicxulub impact, Mexico); and ii) gradual mass extinction, compatible with the paleoenvironmental effects of massive, long-lasting volcanism (Deccan Traps, India). Over the years, a lot of evidence has been proposed supporting one hypothesis or the other, highlighting isotopic (δ18O, δ13C, 87Sr/86Sr) as well as taphonomic, biostratigraphic, quantitative (relative and/or absolute abundance), phylogenetic, and even teratological. We review previous planktic foraminiferal and stable isotope studies, and provide new quantitative and statistical tests from two pelagic sections: the El Kef section (Tunisia), recognized as the most continuous and expanded lowermost Danian section worldwide, and the Sidi Ziane section (Algeria), affected by relevant hiatus in the lower Danian. The results indicate that all the latest Maastrichtian planktic foraminiferal species except those of Guembelitria went extinct exactly at the KPB, supporting the hypothesis of an almost total extinction. In the light of this new evidence, we maintain that the Maastrichtian planktic foraminiferal specimens found worldwide in lower Danian samples could be the result of similar reworking and vertical mixing processes to those at El Kef and Sidi Ziane.

BackgroundLeishmaniasis is endemic in Tunisia and presents with different clinical forms, caused by the species Leishmania infantum, Leishmania major, and Leishmania tropica. The life cycle of Leishmania is complex and involves several phlebotomine sand fly vectors and mammalian reservoir hosts. The aim of this work is the development and evaluation of a high-resolution melting PCR (PCR-HRM) tool to detect and identify Leishmania parasites in wild and domestic hosts, constituting confirmed (dogs and Meriones rodents) or potential (hedgehogs) reservoirs in Tunisia.MethodsUsing in vitro-cultured Leishmania isolates, PCR-HRM reactions were developed targeting the 7SL RNA and HSP70 genes. Animals were captured or sampled in El Kef Governorate, North West Tunisia. DNA was extracted from the liver, spleen, kidney, and heart from hedgehogs (Atelerix algirus) (n = 3) and rodents (Meriones shawi) (n = 7) and from whole blood of dogs (n = 12) that did not present any symptoms of canine leishmaniasis. In total, 52 DNA samples were processed by PCR-HRM using both pairs of primers.ResultsThe results showed melting curves enabling discrimination of the three Leishmania species present in Tunisia, and were further confirmed by Sanger sequencing. Application of PCR-HRM assays on reservoir host samples showed that overall among the examined samples, 45 were positive, while seven were negative, with no Leishmania infection. Meriones shawi were found infected with L. major, while dogs were infected with L. infantum. However, co-infections with L. major/L. infantum species were detected in four Meriones specimens and in all tested hedgehogs. In addition, multiple infections with the three Leishmania species were found in one hedgehog specimen. Sequence analyses of PCR-HRM products corroborated the Leishmania species found in analyzed samples.ConclusionsThe results of PCR-HRM assays applied to field specimens further support the possibility of hedgehogs as reservoir hosts of Leishmania. In addition, we showed their usefulness in the diagnosis of canine leishmaniasis, specifically in asymptomatic dogs, which will ensure a better evaluation of infection extent, thus improving elaboration of control programs. This PCR-HRM method is a robust and reliable tool for molecular detection and identification of Leishmania and can be easily implemented in epidemiological surveys in endemic regions.Graphical

Contribution of foraminifers in biostratigraphy and paleoecology interpretations of the basal Eocene from the phosphatic Sra Ouertaine basin, in the southern Tethys(Tunisia)

The overall objective of this study is to evaluate and correct the Tropical Rainfall Measuring Mission (TRMM) 3B42 algorithm for Northern Tunisia focusing on heavy rainfall events. Two types of correction methods are tested. The first is the combined scheme (CoSch) which was applied in two different ways. CoSch (1) combines satellite data with interpolated in situ data. However, CoSch (2) combines satellite data with a not interpolated in situ map where the pixel value is randomly selected from the rainfall stations belonging this pixel. The second type of correction is the best linear unbiased estimator. The study period is from January 2007 to August 2009. The in situ database is composed of an average of 318 rain gauges. Heavy events are defined as those daily events exceeding 50 mm/day for at least one station. A total of 77 heavy rainfall events result from this selection criterion; 35 events were recorded during the dry period (May to October) and 42 during the wet season (November to April). We first investigate the boxplots of the various evaluation indicators for raw TRMM. The best achievement is for moderate events. The worst performance is for very light and light events. Moreover, we noticed that raw TRMM estimates perform better during wet season. The error decomposition underlined that the highest underestimated values are localized in orographic areas in Le Kef, also in Cap Bon. However, the rainfall overestimation appeared in the central part of the study area (Bizerte and Zaghouan). About the bias correction method comparison, CoSch (1) performance showed a stronger correction than the unbiased estimator which outperforms CoSch (2). As for raw TRMM, CoSch (1) reports better correlation during wet season. The correction of probability of detection (POD) is more important for the wet season reaching 0.9 by the CoSch (1) and unbiased estimator methods. The threat score (TS) coefficients are found not sensitive to the season whatever the correction method.

Medicago polymorpha L. is a herbaceous legume that can be a useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation in five populations of M. polymorpha collected from different ecogeographic regions in Tunisia using eight SSR markers. The transferability of 112 SSR markers distributed on the eight chromosomes of M. truncatula Gaertn. showed that 50 SSR markers could be amplified in M. polymorpha. Among these 50 SSR markers, eight (8) markers were polymorphous. A high level of polymorphism (126 polymorphic alleles with an average of 5.3 alleles per locus) and a moderate level of genetic diversity were found in all the studied populations, with observed and expected heterozygosities averaging between 0 and 0.69, respectively. Results from analysis of molecular variance (AMOVA) revealed that the most variation was found within populations (76%). Moderate levels of population differentiation (FST = 0.12 to 0.19) accompanied by a high rate of gene flow between populations (Nm = 1.08 to 1.83) were recorded. This molecular differentiation (FST) was not dependent on geographical distances (r = 0.395, p = 0.524), suggesting that studied populations are not geographically isolated. Our results showed that studied populations were clustered into three groups. A first group is formed by the populations of El Kef (TNP7) and Bulla Regia (TNP9), a second group of Enfidha (TNP1) and a third group of Soliman (TNP8) and Mateur (TNP11). Results obtained in our study could be helpful for breeders considering introduction of some lines of this species into M. polymorpha breeding programs.

Tan spot, caused by the necrotrophic fungus Pyrenophora tritici-repentis, is an economically serious disease worldwide. However, little has been learned about the importance of this disease in Tunisia. This study was carried out to determine the distribution of tan spot in nine districts of Northern Tunisia and to assess its prevalence, incidence and severity according to the prospected districts and the most cultivated varieties. Surveys of 414 wheat fields were conducted in 2016, 2017 and 2018. Tan spot was detected in all prospected districts with a frequency of 37%, 64% and 89% in 2016, 2017 and 2018, respectively. Based on prevalence, incidence and severity data, the disease was especially important in Manouba and Zaghouan in 2016, in Bizerte, Ariana, Jendouba, Siliana and El Kef in 2017, and in all visited districts except for Siliana in 2018. It was more serious on durum than on bread wheat. The commercial durum wheat varieties ‘Maali’, ‘Karim’ and ‘Razzak’ were the most grown in Northern districts. ‘Maali’ was the most susceptible during the three years, while ‘Karim’ and ‘Razzak’ showed susceptible reaction only during two years; 2016 and 2017 for the first and 2017 and 2018 for the second. The recently introduced cultivars ‘Carioca’ and ‘Monastir’ and the bread wheat varieties ‘Utique’ and ‘Haidra’ were less susceptible than old improved durum wheat varieties. These findings are useful to farmers to choose most tolerant varieties in their growing area.

Calcareous nannofossil biostratigraphy reveals a continuous depositional record through the Upper Cretaceous‒Paleocene succession in the Zagros Basin; southwest Iran (southeastern part of the Neo-Tethys), especially through the Cretaceous‒Paleogene and the Danian‒Selandian boundaries. The upper part of the Gurpi Formation and the lower part of the Pabdeh Formation were investigated. Calcareous nannofossil assemblages from these formations are characterized by abundant, diverse and moderate- to well-preserved forms with Tethyan affinities. This study concentrates on two sections (the Gurpi and Kabir-Kuh sections) in the west and southwest of Iran in the Zagros Basin, which span biozones CC25c/UC20bTP to NP6/NTp10B in the Kabir-Kuh and CC25b/UC20aTP to NP5/NTp9 in the Gurpi section. Late Maastrichtian nannofossil abundance and diversity in the studied intervals is high with a sharp decrease slightly above the K/Pg boundary. The K/Pg boundary interval is marked by the FO of Biantholithus sparsus, an acme of the dinoflagellate cyst Thoracosphaera operculata, a notable decrease in Cretaceous calcareous nannofossil abundance and diversity, and the appearance of new Paleocene taxa. An increased abundance of Thoracosphaera is recorded similar to that in the El Kef (Global Stratotype Section and Point, GSSP, for the K/Pg boundary), Ellés (auxiliary section), Egypt, Atlantic Ocean and sections elsewhere in the world. Reworked Cretaceous calcareous nannofossils are recorded from the base of the Danian along with the new Paleocene species. The Danian‒Selandian boundary is marked by the appearance of Lithoptychius ulii, Lithoptychius pileatus and Lithoptychius janii at the base of NTp8C; this level is also marked by the second radiation of Fasciculithus. Azam Mahanipour [a_mahanipour@uk.ac.ir], Department of Geology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran; Mohammad Parandavar [parandavar.m@gmail.com], Paleontological Research Department, Exploration Directorate of Oil and Gas, NIOC, Tehran; Mohamed Youssef [myousefgeology@gmail.com], Department of Geology, Faculty of Science, South Valley University, 83523 Qena, Egypt.

Mercury linked to Deccan Traps volcanism, climate change and the end-Cretaceous mass extinction

Campanian to Danian dinoflagellate cyst assemblages from the southwestern Tethyan margin (Tattofte section, western External Rif, Morocco): Biostratigraphic and paleobiogeographic interpretations