Large Time Differences Research Articles

Evolution of reproductive isolation as a by-product of divergent life-history evolution in laboratory populations of Drosophila melanogaster.

We show that two complementary asymmetric isolating mechanisms, likely mediated by divergence in body size, underlie the evolution of incipient reproductive isolation between a set of Drosophila melanogaster populations selected for rapid development and their ancestral controls. Selection has led to great reduction in body size in the fast developing lines. Small males belonging to fast developing lines obtain few matings with large control females, both in presence and absence of large control line males, giving rise to unidirectional, premating isolation caused by sexual selection. Conversely, small selected line females suffer greatly increased mortality following mating with large control males, causing unidirectional postcopulatory prezygotic isolation. We discuss preliminary evidence for evolution of reduced male harm caused to females upon mating in the fast developing lines, and speculate that the females from these lines have coevolved reduced resistance to male harm such that they can no longer resist the harm caused by males from control lines. This potentially implicates differing levels of sexual conflict in creating reproductive barrier between the selected line females and the control males. We also show that a large difference in development time is not sufficient to cause postzygotic incompatibilities in the two sets of populations reaffirming the belief that prezygotic isolation can evolve much earlier than postzygotic isolation.

The association between survey timing and patient-reported experiences with hospitals: results of a national postal survey

BackgroundResearch on the effect of survey timing on patient-reported experiences and patient satisfaction with health services has produced contradictory results. The objective of this study was thus to assess the association between survey timing and patient-reported experiences with hospitals.MethodsSecondary analyses of a national inpatient experience survey including 63 hospitals in the 5 health regions in Norway during the autumn of 2006. 10,912 (45%) patients answered a postal questionnaire after their discharge from hospital. Non-respondents were sent a reminder after 4 weeks. Multilevel linear regression analysis was used to assess the association between survey timing and patient-reported experiences, both bivariate analysis and multivariate analysis controlling for other predictors of patient experiences.ResultsMultivariate multilevel regression analysis revealed that survey time was significantly and negatively related to three of six patient-reported experience scales: doctor services (Beta = -0.424, p< 0.05), information about examinations (Beta = -0.566, p < 0.05) and organization (Beta = -0.528, p < 0.05). Patient age, self-perceived health and type of admission were significantly related to all patient-reported experience scales (better experiences with higher age, better health and routine admission), and all other predictors had at least one significant association with patient-reported experiences.ConclusionsSurvey time was significantly and negatively related to three of the six scales for patient-reported experiences with hospitals. Large differences in survey time across hospitals could be problematic for between-hospital comparisons, implying that survey time should be considered as a potential adjustment factor. More research is needed on this topic, including studies with other population groups, other data collection modes and a longer time span.

The potential impact of immunization campaign budget re-allocation on global eradication of paediatric infectious diseases.

BackgroundThe potential benefits of coordinating infectious disease eradication programs that use campaigns such as supplementary immunization activities (SIAs) should not be over-looked. One example of a coordinated approach is an adaptive "sequential strategy": first, all annual SIA budget is dedicated to the eradication of a single infectious disease; once that disease is eradicated, the annual SIA budget is re-focussed on eradicating a second disease, etc. Herd immunity suggests that a sequential strategy may eradicate several infectious diseases faster than a non-adaptive "simultaneous strategy" of dividing annual budget equally among eradication programs for those diseases. However, mathematical modeling is required to understand the potential extent of this effect.MethodsOur objective was to illustrate how budget allocation strategies can interact with the nonlinear nature of disease transmission to determine time to eradication of several infectious diseases under different budget allocation strategies. Using a mathematical transmission model, we analyzed three hypothetical vaccine-preventable infectious diseases in three different countries. A central decision-maker can distribute funding among SIA programs for these three diseases according to either a sequential strategy or a simultaneous strategy. We explored the time to eradication under these two strategies under a range of scenarios.ResultsFor a certain range of annual budgets, all three diseases can be eradicated relatively quickly under the sequential strategy, whereas eradication never occurs under the simultaneous strategy. However, moderate changes to total SIA budget, SIA frequency, order of eradication, or funding disruptions can create disproportionately large differences in the time and budget required for eradication under the sequential strategy. We find that the predicted time to eradication can be very sensitive to small differences in the rate of case importation between the countries. We also find that the time to eradication of all three diseases is not necessarily lowest when the least transmissible disease is targeted first.ConclusionsRelatively modest differences in budget allocation strategies in the near-term can result in surprisingly large long-term differences in time required to eradicate, as a result of the amplifying effects of herd immunity and the nonlinearities of disease transmission. More sophisticated versions of such models may be useful to large international donors or other organizations as a planning or portfolio optimization tool, where choices must be made regarding how much funding to dedicate to different infectious disease eradication efforts.

Energy spectra and bubble velocity distributions in pseudo-turbulence: Numerical simulations vs. experiments

Direct numerical simulations (DNS) are performed to study the behavior of a swarm of rising air bubbles in water, employing the front tracking method, which allows to handle finite-size bubbles. The swarms consist of monodisperse deformable 4 mm bubbles with a gas fraction of 5% and 15%. This paper focuses on the comparison of the liquid energy spectra and bubble velocity probability density functions (PDFs) with experimental data obtained by phase-sensitive constant-temperature anemometry (CTA) and three-dimensional particle tracking velocimetry (PTV), respectively. The numerical simulations confirm that the spectra of the velocity fluctuations driven by the rising bubbles follow a power law with slope close to −3, supporting the idea that the dissipation of the bubble wake is the origin of this spectral scaling, as previously proposed by Lance and Bataille. The computed PDFs of the bubble velocity show non-Gaussian features, as is also observed in the experiments. The agreement with experimental measurements is especially good in the peak region, whereas the tails of the experimental PDFs show more intermittency in comparison to the numerical results. This can be explained by the lack of large-scale flow structures in the simulations, and by the large difference in measurement time.

Agronomic studies on irrigated soybean in southern New South Wales. I. Phenological adaptation of genotypes to sowing date

Serial sowing date studies were used to examine the response of a diverse range of soybean genotypes to sowing date in the Murrumbidgee Irrigation Area (MIA). The aim was to explore the scope to improve the flexibility for rotating irrigated summer soybean crops with winter cereals by broadening the range of potential sowing dates. Serial sowings of diverse genotypes were made in small plots at intervals of ~7 days (2006–07) or 10 days (2007–08) from late November to late January (2006–07) or mid-February (2007–08) and the dates of flowering and maturity recorded. Simple linear models relating rate of development towards flowering to photo-thermal variables indicated that large differences in time to flowering between genotypes, sowing dates, and years could be explained in terms of differences in genotype sensitivity to mean photoperiod and/or mean daily temperature between sowing and flowering. In general, warmer temperatures hastened and longer days delayed flowering, consistent with quantitative short-day photoperiodic response. The earliest flowering genotypes were insensitive to the prevailing photoperiods, and their smaller variations in time to flower over sowing dates and years were related to temperature. Conversely, later flowering genotypes were progressively more sensitive to photoperiod, with flowering occurring later and being more responsive to sowing date. In both seasons, late maturing genotype × sowing date combinations suffered cold temperature damage and frosting. For those genotype × sowing date combinations that were physiologically mature before the first frost, crop duration was a linear function (r2 = 0.86**) of time to flowering. In 2007–08, measurements were also made at maturity of total standing dry matter (TDM), seed yield, and seed size. For those genotype × sowing date combinations that matured before the first frost, TDM was largely a linear function (r2 = 0.83**) of crop duration, while seed yield was strongly related (r2 = 0.86**) to TDM. Exposure to cold temperatures before physiological maturity reduced seed size and harvest index. Using the generalised relations developed in these studies, it was concluded that commercial yields may be possible for irrigated soybean crops in the MIA sown in December or possibly later. These options are evaluated in greater detail in the companion paper, using large-scale agronomic trials of a subset of adapted genotypes.

Fibrillatory Conduction in the Atrial Free Walls of Goats in Persistent and Permanent Atrial Fibrillation

Over a time course of months, the stability of atrial fibrillation (AF) gradually increases and the efficacy of pharmacological cardioversion declines both in humans and in animal models. Changes in fibrillatory conduction over this period largely are unexplored. Goats were instrumented with an atrial endocardial pacemaker lead and a burst pacemaker. AF was maintained for 3 weeks (short-term AF [ST], n = 10) or 6 months (long-term AF [LT], n = 7). AF could be cardioverted pharmacologically at the early time point (persistent AF), but not at the later time point (permanent AF). At follow-up, a high-resolution mapping electrode was used to record epicardial conduction patterns in the free walls of the right atrium (RA) and left atrium (LA). A new method for mapping of fibrillation waves was used to describe AF conduction patterns. Wavefronts propagated uniformly during slow pacing in both groups, although conduction velocity was significantly lower in the LT group (LA, 93 ± 14 versus 72 ± 10 cm/s; RA, 94 ± 8 versus 78 ± 8 cm/s). Median AF cycle length (AFCL) was not significantly different between the groups. However, the LT group showed highly complex activation patterns during AF, with an increased number of simultaneously propagating waves (LT group RA, 8.4 ± 3.0 waves/AFCL; LA, 12.8 ± 2.4 waves/AFCL; versus ST group RA, 4.3 ± 2.2 waves/AFCL; LA, 4.5 ± 2.5 waves/AFCL). Fibrillation waves in the LT group showed pronounced dissociation with large activation time differences. The incidence of waves newly appearing within the recording area also was increased in both atria. These alterations in conduction were accompanied by myocyte hypertrophy and increased endomysial fibrosis. Long-term AF in goats leads to dissociated conduction in the atrial free walls that may contribute to increased AF stability.

Entrainment, annual circulation and groundwater inflow in a chain of lakes as inferred by stable 18O isotopic signatures in the water column

The stable oxygen isotopic signature δ18O of water has been proved to be a useful marker in hydrological lake studies. However, it is applied only sporadically to determine the extent of mixis and stratification, which is vital for all hydrological and limnological studies. We present a study of the changes in δ18O, temperature and Schmidt stability in the water column of three adjacent lakes of very different mixis types (polymictic to meromictic) over almost one year (May 2008 to April 2009). The response of δ18O to important hydrologic processes (entrainment, stratification, circulation, groundwater inflow) and weather influences (cooling period, ice cover and melt water inflow) is discussed. The lakes are part of the Osterseen chain of lakes south of Munich, Germany. Although hydrologically connected, these lakes show various mixis types (polymictic to meromictic) due to large differences in size, groundwater inflow and water renewal time. Polymixis and the strong subsurface inflow of groundwater in Lake Waschsee (25.6 × 103 m3) were indicated by the same trends in the δ18O signature throughout all water layers and by the mean overall signature (-9.94‰) being very close to δ18O of local groundwater (-10.01‰ ± 0.06). δ18O signatures of the larger dimictic Lake Fohnsee (2298.3 × 103 m3) revealed a highly significant trend towards lower values of δ18O in its hypolimnion, indicating inflow of groundwater. A cooling period during the summer stratification characterised by high wind speeds resulted in a considerable drop of lake surface temperatures and Schmidt stability (up to 25%) in lakes Fohnsee and Eishaussee and was followed by a deepening of the mixed upper water layer and entrainment of hypolimnetic water layers. This was clearly shown by a signal change in deeper water layers formerly constant in δ18O. The permanent meromixis present in Lake Eishaussee (297.0 × 103 m3) could also be confirmed by isotopic signatures, as bottom water layers remained significantly isolated in δ18O from the remaining water column over the whole study period. We summarize that the oxygen isotopic signature of water is an easy to interpret, excellent indicator of important hydrologic processes in a lake and can readily be integrated into routine sampling. The present findings will further contribute to the analysis of hydrological data as well as to the interpretation of paleoclimatic reconstructions using proxies of lake water δ18O.

Analytical model of internally coupled ears

Lizards and many birds possess a specialized hearing mechanism: internally coupled ears where the tympanic membranes connect through a large mouth cavity so that the vibrations of the tympanic membranes influence each other. This coupling enhances the phase differences and creates amplitude differences in the tympanic membrane vibrations. Both cues show strong directionality. The work presented herein sets out the derivation of a three dimensional analytical model of internally coupled ears that allows for calculation of a complete vibration profile of the membranes. The analytical model additionally provides the opportunity to incorporate the effect of the asymmetrically attached columella, which leads to the activation of higher membrane vibration modes. Incorporating this effect, the analytical model can explain measurements taken from the tympanic membrane of a living lizard, for example, data demonstrating an asymmetrical spatial pattern of membrane vibration. As the analytical calculations show, the internally coupled ears increase the directional response, appearing in large directional internal amplitude differences (iAD) and in large internal time differences (iTD). Numerical simulations of the eigenfunctions in an exemplary, realistically reconstructed mouth cavity further estimate the effects of its complex geometry.

Effect of the fatigue data editing technique associated with finite element analysis on the component fatigue design period

The present study concerns development of accelerated fatigue design time based finite element analysis (FEA) through a combination of the fatigue data editing (FDE) technique and FEA. Country road, pavement and highway fatigue data histories were collected using a fatigue data acquisition system. The component durability was evaluated using both original and editing fatigue data signals. Both signals showed a large difference in the CPU time. Furthermore, the histories were successfully edited by presenting the predicted amount of the fatigue damage as the original histories. As a consequence of application for automotive lower suspension arm, this technique can be applied in performing a fatigue history editing, especially in compression of data set. Briefly, design time was saved based on FDE technique associated with FEA.

Working hours and fatigue of Japanese flight attendants (FA).

There have been some reports concerning high complaint rates of fatigue or fatigue-related symptoms including lower back pain in flight attendants (FA). Thus, the relations of working conditions with work stress and fatigue symptoms were studied chiefly by focusing on working hours. From analysis of the time-table and fatigue symptoms of workers on international flights, it was suspected that there were some work-related factors jointly causing serious FA fatigue symptoms; night time and early morning work, long flight hours and a large time difference, thus disturbing their biological rhythms. On domestic flights, showing up early in the morning and debriefing late in the night were often observed together with a highly irregular FA time schedule. By statistical analyses, some factors including long working hours, frequent landing and late debriefing hours were considered to contribute significantly to the high fatigue complaint rates. Thus, it should be emphasized that many countermeasures are necessary to improve FA working conditions including working hours, rest on the airplane (ONO et al., 1990) and sleep during layover, in order to reduce their work stress and fatigue symptoms.

SU‐DD‐A3‐03: How Accurately Can the Internal Target Volume (ITV) from a Free‐Breathing Cone Beam Computed Tomography (FB‐CBCT) Scan Be Used for Target Verification?

Purpose: An internal target volume (ITV) is often identified by acquiring a free‐breathing cone‐beam computed tomography scan (FB‐CBCT) to verify patient setup of tumors affected by respiratory motion. However, its accuracy is not well understood. The purpose of this study is to characterize the potential underestimation of the ITV when a FB‐CBCT is acquired with irregular respiratory cycles. Methods &amp; Materials: Five patient respiratory profiles were programmed into a 4D Dynamic Thorax (CIRS Model 008) phantom containing a spherical target, 2cm in diameter. The superior‐inferior motion was 3cm with respiratory cycles approximately 5 seconds in length. Five 360° CBCT scans of each profile were acquired using a gantry mounted kV imaging system. A FB‐CBCT, as well as a 4DCBCT of 10 phase bins with 10% phase windows were reconstructed for each profile. Inspiration (0%) and expiration (50%) phase images were compared to FB‐CBCT images. The ITV of each FB‐CBCT displayed a sharp and a smeared region. The percent reduction in contrast‐to‐noise ratio (CNR) between these regions was calculated, as well as the ratio of the average time spent in inspiration versus expiration. The relationship between contrast reduction and the ratio of time spent per phase was investigated. Results: For the five profiles studied, the CNR reductions ranged from 37% to 70%, corresponding to ratios of average time spent in inspiration versus expiration that ranged from 0.42 to 0.12, respectively. As the difference between time spent in expiration versus inspiration increased, the reduction in contrast also generally increased (r2=0.873). Conclusions: The observed loss of contrast in the ITV for irregular respiratory cycles may lead to the potential underestimation of this volume obtained from a FB‐CBCT. Extra caution is required when using an ITV for on‐board target localization when respiratory cycles exhibit large time differences between inspiration and expiration periods.

Efficient adaptive three-dimensional phase field simulation of free dendritic growth under natural convection

A great challenge in simulating dendritic crystal growth from various supercoolings is the large difference in time and length scales. Dendrite growth is also known to be affected by natural convection. By using adaptive mesh refinement and rescaling, the effects of gravity and its orientation on tip growth rate and dendrite morphology are investigated. Our simulation results are consistent with previous theories and experimental observations.

Agronomic performance of late-season rice under different tillage, straw, and nitrogen management

In double rice-cropping system in China, zero tillage in late-season rice with straw return from early season rice is an emerging technology for saving inputs, shortening the lag time between rice crops, avoiding straw burning, and conserving natural resources. The objective of this 2-year field study was to determine the effects of tillage and straw return on N uptake, grain yield, and N use efficiency of late-season rice. Treatments were arranged in a split-plot design with four combinations of tillage and straw return as main plots and three N management practices as subplots. Tillage was either conventional soil puddling or zero tillage with newly harvested crop residue from early season rice either removed or placed on the soil surface without incorporation. The N treatments were zero-N control, site-specific N management (SSNM), and farmers’ N-fertilizer practice (FFP). Straw return regardless of tillage or N management did not reduce rice yield. In the second year, straw return significantly increased grain yield in the zero-N control. Chlorophyll meter readings at heading and total N uptake at maturity were higher with straw return in the zero-N control, suggesting that straw provides nutrients to rice in the late growing period. Zero tillage did not reduce N uptake, grain yield, and N use efficiency compared with conventional tillage. Despite large differences in timing and rate of N application between FFP and SSNM, these two N treatments resulted in comparable N uptake and grain yield of late-season rice regardless of tillage and straw return. These results suggest that zero tillage after early rice with straw return could replace conventional tillage for late rice in the double rice-cropping system in China.

Cross-training workers in Dual Resource Constrained systems with heterogeneous processing times

In this paper, we explore the effect of cross-training workers in Dual Resource Constrained (DRC) systems with machines having different mean processing times. By means of queuing and simulation analysis, we show that the detrimental effects of pooling (cross-training) previously found in single resource constrained (SRC) heterogeneous systems are also apparent in DRC heterogeneous systems. Fully cross-training workers in DRC heterogeneous systems is only beneficial if the differences between mean processing times are not too large, otherwise cross-training should be pursued within homogeneous subgroups of machines. Due to the limited machine availability, DRC systems are unable to use some of the potential assignment flexibility from cross-trained workers (pooled queues) that can be used in SRC systems. However, it appears that this restriction in the DRC system may even improve the system mean flow (waiting) time performance compared to the SRC system for relatively large differences in processing time. Finally, in fully flexible multiple server queuing systems, restricting the assignment flexibility by applying a decentral when-rule (i.e. a commonly used labour assignment rule in practice and research) instead of a central when-rule also seems to improve the mean flow time performance under processing time differences.

Sleep Patterns in High School and University Students: A Longitudinal Study

We performed a longitudinal study to investigate whether changes in social zeitgebers and age alter sleep patterns in students during the transition from high school to university. Actimetry was performed on 24 high-school students (mean age ± SD: 18.4 ± 0.9 yrs; 12 females) for two weeks. Recordings were repeated in the same subjects 5 yrs later when they were university students. The sleep period duration and its center, the mid-sleep time, and total sleep time were estimated by actimetry. Actigraphic total sleep time was similar when in high school and at the university on school days (6.31 ± 0.47 vs. 6.45 ± 0.80 h, p = ns) and longer on leisure days by 1.10 ± 1.10 h (p < 0.0001 vs. school days) when in high school, but not at the university. Compared to the high school situation, the mid-sleep time was delayed when at the university on school days (03∶11 ± 0.6 vs. 03∶55 ± 0.7 h, p < 0.0001), but not on leisure days. Individual mid-sleep times on school and leisure days when in high school were significantly correlated with the corresponding values 5 yrs later when at the university (r = 0.58 and r = 0.55, p < 0.05, respectively). The large differences in total sleep time between school and leisure days when students attended high school and the delayed mid-sleep time on school days when students attended university are consistent with a circadian phase shift due to changes in class schedules, other zeitgebers, and lifestyle preferences. Age-related changes may also have occurred, although some individuality of the sleep pattern was maintained during the 5 yr study span. These findings have important implications for optimizing school and work schedules in students of different age and level of education. (Author correspondence: pneubloc@usz.uzh.ch)

Managing anthelmintic resistance: Modelling strategic use of a new anthelmintic class to slow the development of resistance to existing classes

AIM: To test the hypothesis that a single strategic treatment with a new class of anthelmintic could slow the development of resistance to existing classes of anthelmintic. METHODS: An existing model was used to simulate nematode parasite dynamics and the development of anthelmintic resistance. Variations on a five-drench preventive programme of treatments for lambs, in which either zero, the first, third or fifth treatment was substituted with a different class of drug, were compared for the time to reach treatment failure (defined as efficacy <95%). The sensitivity to variations in the death rate of adult worms, that varied from 1 to 5%, and the dominance of resistance genes were also assessed. RESULTS: Replacing one of the five treatments with a different class of anthelmintic almost always slowed the development of resistance, and was never worse than using the same drug for all treatments. Further, there were large differences in the relative time to treatment failure depending on which treatment was substituted. Changing the first treatment always had the least benefit, whereas changing the fifth treatment always had the greatest. This pattern was independent of the daily death rate of adult worms, and was not influenced by the dominance of resistance under treatment. CONCLUSIONS: The results indicated that strategic substitution of a single treatment with a new class of anthelmintic, at the end of a series of preventive treatments to lambs using an existing class, could slow the further development of resistance to the latter. This strategic use of a new anthelmintic class has the potential to greatly extend the life of existing anthelmintics if these are still effective.

SLEEP PATTERNS IN HIGH SCHOOL AND UNIVERSITY STUDENTS: A LONGITUDINAL STUDY

We performed a longitudinal study to investigate whether changes in social zeitgebers and age alter sleep patterns in students during the transition from high school to university. Actimetry was performed on 24 high-school students (mean age ± SD: 18.4 ± 0.9 yrs; 12 females) for two weeks. Recordings were repeated in the same subjects 5 yrs later when they were university students. The sleep period duration and its center, the mid-sleep time, and total sleep time were estimated by actimetry. Actigraphic total sleep time was similar when in high school and at the university on school days (6.31 ± 0.47 vs. 6.45 ± 0.80 h, p = ns) and longer on leisure days by 1.10 ± 1.10 h (p < 0.0001 vs. school days) when in high school, but not at the university. Compared to the high school situation, the mid-sleep time was delayed when at the university on school days (03∶11 ± 0.6 vs. 03∶55 ± 0.7 h, p < 0.0001), but not on leisure days. Individual mid-sleep times on school and leisure days when in high school were significantly correlated with the corresponding values 5 yrs later when at the university (r = 0.58 and r = 0.55, p < 0.05, respectively). The large differences in total sleep time between school and leisure days when students attended high school and the delayed mid-sleep time on school days when students attended university are consistent with a circadian phase shift due to changes in class schedules, other zeitgebers, and lifestyle preferences. Age-related changes may also have occurred, although some individuality of the sleep pattern was maintained during the 5 yr study span. These findings have important implications for optimizing school and work schedules in students of different age and level of education. (Author correspondence: pneubloc@usz.uzh.ch)

Nitrate variability in hydrological flow paths for three mid‐Appalachian forested watersheds following a large‐scale defoliation

Nitrate (NO3−) leakage from forested watersheds due to disturbance is a well documented but not well understood process that can contribute to the degradation of receiving waters through eutrophication. Several studies have shown that large‐scale defoliation and deforestation events in small forested watersheds in the eastern United States cause immediate and dramatic increases in NO3− flux to steams, with large differences in recovery time. Water quality and discharge data collected from 1992 to 2004 following a large‐scale gypsy moth defoliation were used to investigate hydrological controls on long‐term NO3− leakage from three forested watersheds in Shenandoah National Park, Virginia. During storm events, a conventional two‐component hydrograph separation in conjunction with an inverse solution technique was employed to determine the concentrations of NO3− in groundwater and soil water. Following defoliation, groundwater NO3− concentrations declined exponentially with a distinct seasonal pattern. A rank‐order relationship between the rate constants associated with the exponential declines in groundwater NO3− concentrations and groundwater recession constants indicates a hydrological control on long‐term watershed recovery for these defoliated systems. Comparisons to deforested systems in Hubbard Brook, New Hampshire, and Coweeta, North Carolina, indicate hydrological controls are similarly present. Biogeochemical differences, however, need to be considered to account for the more attenuated recovery observed in defoliated systems. No long‐term trend was found in the model‐derived soil water NO3− concentrations, which suggests the presence of some form of rate limitation on the transformation of the nitrogen pool introduced during the disturbance and/or reduced nutrient uptake due to tree mortality.

Disagreement in physical activity assessed by accelerometer and self-report in subgroups of age, gender, education and weight status.

BackgroundThe purpose of this study is to compare self-reported time (by questionnaire) and objectively measured time (by accelerometer) spent on physical activity at moderate (MPA) and vigorous intensity (VPA) in subgroups of age, gender, education and weight status.MethodsIn total, 236 adolescents (aged 12–18) and 301 adults (aged 22–40), completed the questionnaire and wore an accelerometer for two weeks.ResultsAdolescents reported exceptionally more time spent on MPA (mean difference 596 ± 704 min/wk) and VPA (mean difference 178 ± 315 min/wk) than was assessed objectively by the accelerometer. Based on the questionnaire, high educated adolescents spent more time on MPA (205 min/wk, p = 0.002) and VPA (120 min/wk, p = 0.01) than low educated adolescents, but according to the accelerometer they spent less time on MPA (149 min/wk, p = 0.001) and VPA (47 min/wk, p = 0.001). Among adults there was moderate agreement between self-reported time and objectively measured time spent on MPA, but in general the reported time spent on MPA (mean difference 107 ± 334 min/wk) and VPA (mean difference 169 ± 250 min/wk) exceeded the time measured with the accelerometer. Overweight adults reported significantly more VPA (57 min/wk, p = 0.04) than normal weight adults, but this was not confirmed by the accelerometer data.ConclusionWe observed large differences in time spent on MPA and VPA measured by questionnaire and accelerometer in adolescents but reasonably good agreement in adults. Differences between methods varied by gender, education and weight status. This finding raises serious questions about the use of questionnaires to quantify MPA and VPA in adolescents. There is a clear need in advanced valid assessments of PA in adolescents.Trial numberISRCTN93896459

Tuning to Interaural Time Difference and Frequency Differs Between the Auditory Arcopallium and the External Nucleus of the Inferior Colliculus

Barn owls process sound-localization information in two parallel pathways, the midbrain and the forebrain pathway. Exctracellular recordings of neural responses to auditory stimuli from far advanced stations of these pathways, the auditory arcopallium in the forebrain and the external nucleus of the inferior colliculus in the midbrain, demonstrated that the representations of interaural time difference and frequency in the forebrain pathway differ from those in the midbrain pathway. Specifically, low-frequency representation was conserved in the forebrain pathway, while it was lost in the midbrain pathway. Variation of interaural time difference yielded symmetrical tuning curves in the midbrain pathway. By contrast, the typical forebrain-tuning curve was asymmetric with a steep slope crossing zero time difference and a less-steep slope toward larger contralateral time disparities. Low sound frequencies contributed sensitivity to contralateral leading sounds underlying these asymmetries, whereas high frequencies enhanced the steepness of slopes at small interaural time differences. Furthermore, the peaks of time-disparity tuning curves were wider in the forebrain than in the midbrain. The distribution of the steepest slopes of best interaural time differences in the auditory arcopallium, but not in the external nucleus of the inferior colliculus, was centered at zero time difference. The distribution observed in the auditory arocpallium is reminiscent of the situation observed in small mammals. We speculate that the forebrain representation may serve as a population code supporting fine discrimination of central interaural time differences and coarse indication of laterality of a stimulus for large interaural time differences.