Labrusca Grapes Research Articles

Ultrasound-assisted extraction of anthocyanins from grape pomace using acidified water: Assessing total monomeric anthocyanin and specific anthocyanin contents

This study aimed to optimize the ultrasound-assisted extraction (UAE) of anthocyanins from oven-dried and freeze-dried Vitis labrusca grape pomace, using acidified water as the solvent. The effects of power density (8.3–16.7 W/mL), pulse interval (0–2 s), and extraction time (1–5 min) on both total and specific anthocyanins were investigated. The findings suggested that acidified water can be a viable alternative to conventional solvents and that oven drying was an effective method for drying the pomace. Using response surface methodology, the study identified power density and extraction time as key factors influencing total anthocyanin content, with extracts reaching contents up to 2.56 mg/g. The analysis using LC-MS identified 14 anthocyanins, while NMR quantified 3 and malvidin diglucoside was generally the most abundant. However, higher power and longer extraction times were found to reduce its content while increasing malvidin monoglucoside content, suggesting ultrasound-induced anthocyanin hydrolysis. In conclusion, this study presents a sustainable method for extracting anthocyanins using acidified water, contributing to the valorization of Vitis labrusca grape pomace for industrial use.

Agrochemical characterization of Vitis labrusca grape pomace and its effect on a soil-plant system

Agrochemical characterization of Vitis labrusca grape pomace and its effect on a soil-plant system

Functional and chemical qualities of Vitis labrusca grape seed oil extracted by supercritical CO2

Grape seed oil, which is extracted with highly toxic organic solvents that are harmful to human health, is produced from tons of grape pomace waste, generated during winemaking. Sometimes, this waste is used to make compost or is burnt, which causes environmental contamination. The functional qualities, antioxidant capacity (AC), α-tocopherol and total phenolic compounds content (TPC) of Black Borgoña (Vitis labrusca) grape seed oil, extracted by supercritical CO2, were evaluated. The high content of linoleic acid (w-6) and monounsaturated fatty acids contributed to the beneficial effect on ​​the functional quality indices, which were 0.20, 0.23, 11.80 for IA, IT and H:H, respectively. In addition, a POV of 6.23 ± 0.08 milliequivalents of peroxide/kg oil and an anisidine index of 2.70 ± 0.05 indicated a good quality oil. Also, a high concentration of α-tocopherol (9.82 ± 0.02 mg/100 g oil) and a high TPC (114.14 ± 3.24 mg GAE/kg oil) were obtained. This study demonstrated that supercritical CO2 extraction is a suitable method for the delivery of a high-quality grape seed oil.

Field performance of five white Pilzwiderstandsfahige (PIWI) cultivars in the south of Brazil

Southern Brazil is the largest grape-producing region in the country. It is characterized by the production of labrusca grapes, because of the difficulty of growing Vitis vinifera. This can be explained by the high occurrence of fungal diseases, especially downy mildew, which is favored by the environmental conditions (high temperatures, relative humidity and rainfall). Pilzwiderstandsfahige (PIWI) are a group of wine-grape cultivars similar to V. vinifera but with resistance to downy and powdery mildew. These cultivars open the possibility of reducing production cost and agrochemical sprays for viticulture in Brazil. In order to achieve this objective, studies related to phenological, productive and qualitative characteristics are necessary. A PIWI cultivar trial was established in 2015, in the city of Videira, Santa Catarina State, Brazil, with five white cultivars. The vines were trained in vertical shoot position (VSP) trellis and pruned in double cordons. The studied cultivars were ‘Felicia’, ‘Calardis Blanc’, ‘Helios’, ‘Bronner’ and ‘Aromera’, grafted on ‘Paulsen 1103’. The variables evaluated were phenology, incidence of downy mildew, yield, number of clusters, average cluster weight, total soluble solids, pH and total titratable acidity. In terms of productivity, the outstanding cultivars were ‘Felicia’ and ‘Calardis Blanc’, with yields above the traditional V. vinifera cultivars. ‘Felicia’ was the earliest cultivar and produced the heaviest clusters. ‘Calardis Blanc’ presented the largest number of clusters, and ‘Aromera’ was the latest cultivar. Soluble solids contents, pH and acidity were adequate in all cultivars. In general, all tested cultivars showed good potential for the studied region.

Improvement of Enzymatic Assisted Extraction Conditions on Anthocyanin Recovery from Different Varieties of V. vinifera and V. labrusca Grape Pomaces

The incomplete anthocyanin extraction during the industrial processes turns grape pomace into an inexpensive source of phenolic compounds. The effects of temperature and enzyme preparation percentage (% E/S) on the anthocyanin recovery from grape pomaces of eight grape varieties were evaluated by their physicochemical characteristics and phenolic composition. A factorial 22 design with center point was used to select the preferred conditions for extraction, and the variables of temperature, enzyme preparation, and their interaction were assessed. The grape skin characteristics affected the anthocyanins’ content and their recovery yield, and different improvement conditions were found for each variety of grape. Anthocyanin extraction from Cabernet Sauvignon—the variety which showed the highest percentage of anthocyanin recovery (over 50%)—was improved. The lowest tested temperature (40 °C) and percentage of preparation enzymatic (0.25% E/S) promoted higher anthocyanin extraction, resulting in a natural food colorant with 2.67 g anthocyanins/100 g grape skin dry basis (db). The extraction improvement allowed for a non-toxic natural extract rich in anthocyanins to be obtained; a natural additive which can be considered for potential food industry use in place of synthetic dyes, especially into acidic matrices.

Phenolic Compounds and Polyamines in Grape-Derived Beverages

Isotopic analyses and chromatographic analysis (phenolic compounds and biogenic amines), can be applied to investigate the functional and nutritional quality in different grape juices and wines. These beverages when produced exclusively from grapes contain bioactive compounds. In this way, the isotopic analysis, as well as the determination of phenolic compounds and biogenic amines were performed with the aim of verify the functional quality of juices and wines produced with Vitis vinifera and Vitis labrusca grapes. The samples that were analyzed consisted of four whole juices, two nectars, two V. vinifera wines, and two V. labrusca wines. Regarding the isotopic analyses, only one nectar, among the beverages studied, presented the addition of sugar from C4 plants. Wines from V. vinifera showed the highest content of biogenic amines and phenols; whereas, the highest content of anthocyanins were found in V. labrusca. The levels of biogenic amines and phenolic compounds were variable between samples, and recommendations for consumers should be made considering several conditions, such as physiological state, age, consumption, among others. Anthocyanins and biogenic amines, as well as isotopic analyses, can be applied as tools to measure the quality of grape derived beverages.

English

In grape must, the aroma is an indication of adequate conservation status. It is able to indicate losses due to thermal degradation, besides representing a great contribution in the properties of the sensorial quality of the grape juices most appreciated by consumers. This study identified the volatile compounds in different must cultivars of Vitis labrusca grapes. The volatile composition was evaluated by the solid phase microextraction method (SPME), using the polydimethylsiloxane/divinylbenzene fiber (PDMS/DVB). The separation and identification of the volatile compounds were carried out through gas chromatography by mass spectrometry (GC-MS). The results showed the identification of forty-four compounds belonging to the following chemical classes: carboxylic acids, alcohol, aldehydes, ketones, furans esters and terpenes. The class of esters was the most numerous compounds identified and is largely responsible for the fresh and fruity aroma of the tested musts. Solid-phase microextraction proved to be a reproducible, sensitive and fast-response technique for the identification of chemical biomarkers in musts of different grape cultivars. Key words: Aroma, gas chromatography by mass spectrometry (GC-MS), headspace, solid phase microextraction method (SPME).

Phenolic Compounds and Antioxidant Activity in Grape Juices: A Chemical and Sensory View

The search for food products that promote health has grown over the years. Phenolic compounds present in grapes and in their derivatives, such as grape juices, represent today a broad area of research, given the benefits that they have on the human health. Grape juice can be produced from any grape variety once it has attained appropriate maturity. However, only in traditional wine producing regions, grape juices are produced from Vitis vinifera grape varieties. For example, Brazilian grape juices are essentially produced from Vitis labrusca grape varieties, known as American or hybrid, as they preserve their characteristics such as the natural flavour after pasteurisation. Grapes are one of the richest sources of phenolic compounds among fruits. Therefore, grape juices have been broadly studied due to their composition in phenolic compounds and their potential beneficial effects on human health, specifically the ability to prevent various diseases associated with oxidative stress, including cancers, cardiovascular and neurodegenerative diseases. Therefore, this review will address grape juices phenolic composition, with a special focus on the potential beneficial effects on human health and on the grape juice sensory impact.

ROOTSTOCKS EFFECTS ON THE PHYSICOCHEMICAL CHARACTERISTICS OF BORDÔ, ISABEL AND IAC 138-22 MÁXIMO MUST AND WINE

ABSTRACT It has been demonstrated that rootstocks can influence grape characteristics, but there are few data in literature about the rootstocks influence in the wine characteristics, especially in wines elaborated with Vitis labrusca or hybrid grapes. This study evaluated the influence of ‘IAC 766’ and ‘106-8 Mgt’ rootstocks on the physicochemical characteristics of ‘Bordô’, ‘Isabel’ and ‘IAC 138-22 ‘Máximo’ musts and wines. The musts were evaluated for pH, soluble solids, total acidity and soluble solids/acidity ratio. The wines were evaluated for density, alcohol content; total, volatile and fixed acidity; pH, dry extract, reducing sugars, reduced dry extract, alcohol in weight/reduced dry extract; free and total sulfur dioxide; anthocyanins, polyphenols index (I 280); polyphenols and total flavonoids and antioxidant activity. The ‘Bordô’, ‘Isabel’ and IAC 138-22 ‘Máximo’ musts had low soluble solids for winemaking. The ‘106-8 Mgt’ rootstock promoted higher dry extract content and reduced dry extract in Bordô wine, and lower total and fixed acidity in IAC 138-22 Máximo wine. In Isabel wine, higher pH was promoted by the ‘IAC 766’ rootstock. Aside from low total acidity of the Bordô wine, all the physicochemical characteristics of the wines studied are in accordance with Brazilian law. There was no effect of rootstocks in the phenolics content and antioxidant activity of wines.

Effects of a warming trend on cool climate viticulture in Michigan, USA.

Historically, Michigan’s climate had mainly three challenges for grape production: growing season temperatures were too low, the growing season was too short and there was too much rain near harvest. However, climate change in the past decades has led to a vastly different landscape that is evolving to meet the new climate. Recently, there has been a significant move from Vitis labrusca (North American) grape plantings to Vitis vinifera (wine grapes) as a consequence of Michigan’s shifting climate. The goal of this study was to analyze the historical shift in climate and its potential future impact on the grape industry. We obtained data climate model projection data from two greenhouse gas (GHG) emission scenarios. First, a multi-linear regression model was built to predict future grape yields (t/ac) using data from the climate model projections. Second, trends in the severity of the three challenges (temperature, season length, precipitation timing) were analyzed. In both GHG scenarios grape yields are seen to improve, but to different extents. The improvement is likely a response to warmer season temperatures canceling out losses to early season frost. Model projections recommend that Michigan’s future climate will be more accommodating for all varieties of grapes. This suggests that grape production will continue to grow, but the landscape will continue to evolve with more emphasis on varieties that are more climatically sensitive to cold temperatures. Climate change has greatly affected Michigan’s viticultural landscape, and will continue to do so in the coming decades.

Color, physicochemical parameters and antioxidant potential of whole grape juices subject to different UV-C radiation doses

ABSTRACT Knowing that moderate stress such as UV radiation can activate defense mechanisms in plants, the use of UV-C radiation appears as hypothesis of a promising technique that would help to stimulate and enhance beneficial compounds for health, through a clean and healthy technology. In this study, the possible induction of secondary metabolism, the increase in the content of phytochemical compounds and physicochemical changes through the use of UV-C radiation were evaluated on whole grape juices produced with Vitis labrusca grapes, cultivar Isabel Precoce. Grapes were harvested, sanitized, exposed to UV-C radiation at doses of 0, 2, 4 and 6 KJ m-2, and then the juices were prepared and packed into amber glass bottles at room temperature. Analyses were performed at 0, 30, 60, 90 and 120 days of storage. Based on results obtained and conditions in which the experiment was performed, UV-C treatment in grapes caused abiotic stress in the fruits, affecting color, titratable acidity, soluble solids/titratable acidity ratio, vitamin C and percentage of protection against oxidation. Application of UV-C did not change levels of phenolic compounds in fruit juices or the percentage of scavenging free radicals, pH and soluble solids.

Phlorizin and sorbitol inVitis labruscagrape juices

In Brazil, grape juice is a not fermented beverage, made from Vitis labrusca varieties and their hybrids. The most common form of adulteration is by the addition of apple juice. The adulterated samples can be identified by specific analysis, since apple juice has some compounds that grape has not. A more simplified and assessible way to determine this kind of adulteration is the phlorizin analysis by high performance liquid chromatography (HPLC). Phlorizin is a phenolic compound that has been used to identify adulterations in juices. Besides phlorizin, it can be seen that adulteration by quantifying the levels of sorbitol, present in apples, but absent in healthy grapes. The content of this compounds in grape juices can vary due to the variety of grape and harvest.This study aimed to analyze phlorizin and sorbitol, in 141 experimental samples of Vitis labrusca grape juices, harvest 2016, of 9 varieties and 43 commercial samples (different vintages). The experimental juices from red grapes showed higher sorbitol content than the white grapes. For sorbitol analysis, the juices of Ives differed statistically of the juices of: Isabella, Concord, Niagara Branca and White Muscat. It was detected the presence of apple juice in 5 commercial grape juices.

Optimization of the HS-SPME-GC/MS technique for determining volatile compounds in red wines made from Isabel grapes (Vitis labrusca)

Brazilian wine production is characterized by Vitis labrusca grape varieties, especially the economically important Isabel cultivar, with over 80% of its production destined for table wine production. The objective of this study was to optimize and validate the conditions for extracting volatile compounds from wine with the solid-phase microextraction technique, using the response surface method. Based on the response surface analysis, it can be concluded that the central point values maximize the process of extracting volatile compounds from wine, i.e., an equilibrium time of 15 minutes, an extraction time of 35 minutes, and an extraction temperature of 30 °C. Esters were the most numerous compounds found under these extraction conditions, indicating that wines made from Isabel cultivar grapes are characterized by compounds that confer a fruity aroma; this finding corroborates the scientific literature.

Autochthonous yeasts with β-glucosidase activity increase resveratrol concentration during the alcoholic fermentation of Vitis labrusca grape must

Since red wine is the main dietary source of resveratrol, a well-known polyphenol that reduces coronary events in humans, different strategies have been employed in winery to achieve resveratrol-enriched wines. Yeasts-endowed β-glucosidase activity enhances free-resveratrol concentration in wine without modifying its composition or sensorial properties. Current assay screened 308 autochthonous yeast strains for β-glucosidase activity employing arbutin, esculin, cellobiose and piceid as substrates. The β-glucosidase-producer yeasts were evaluated in the must of Vitis labrusca Bordô grape to quantify resveratrol concentration before and after alcoholic fermentation. Fourteen yeasts increased the resveratrol concentration up to 102% without any significant difference and nine of these yeast strains also produced high ethanol contents. Four autochthonous Hanseniaspora uvarum β-glucosidase-producer strains showed adequate oenological characteristics and hydrolysed resveratrol-glucosides during the alcoholic fermentation of V. labrusca grape must.

Anthocyanin from Vitis labrusca grape used as sensitizer in DSSC solar cells

Dye sensitized solar cells or “Gratzel” cells are electrochemical devices capable of performing the direct conversion of light into electricity and are less expensive than traditional silicon solar cells. The most efficient dyes for Gratzel cells are those composed by the coordination compounds of ruthenium ions, which have high cost due to the shortage of ruthenium (Ru) element in the Earth’s crust. Thus, the study of dyes with lower cost is crucial for the viability of this technology on a large scale. Natural dyes are easily extracted in addition to being environmentally friendly and presenting reduced cost. Anthocyanins are the most important pigments of vascular plants, providing color to the flowers and fruits. In grapes, anthocyanins are found exclusively in the skin and can be easily extracted with ethanol at room temperature. In this work we used the ethanolic extract of anthocyanins from Vitis labrusca grape in the sensitization of TiO2 electrodes and production of a Gratzel cell. The prepared Gratzel cell presents a short-circuit current of 0.045 mA/cm2, above that of the cell sensitized with commercial N719 dye, which was 0.032 mA/cm2. However, the open circuit voltage was 0.293 V, lower than that sensitized with N719 (0.335 V). The fill factor of the anthocyanin sensitized cell was 46.19 %, higher than that of the N719 sensitized cell, 42.72 %. These results are consistent with the greater absorption of light radiation observed in the cell with grape anthocyanins in comparison with the cells sensitized with N719 dye.

Acceptability and preference drivers of red wines produced from Vitis labrusca and hybrid grapes

Wines produced from non-Vitis vinifera varieties have great economic importance in Brazil and represent more than 80% of the national production, but scientific information regarding the quality of these wines is rare. The objective of this research was to determine consumer acceptability, the sensory profile and the chemical composition of the most consumed Brazilian red wines produced with Vitis labrusca and promising hybrid varieties, identifying the parameters that drive the preference of consumers. Commercial wines collected directly from different wineries were evaluated regarding their overall acceptance by 120 consumers. Twelve trained panelists developed the sensory profile of the wines using Quantitative Descriptive Analysis (QDA). Physicochemical analyses carried out to determine the pH, titratable and volatile acidity, total solids, alcohol degree, total phenolics, free SO2 and the reducing sugar contents of the wines. The data was analyzed by ANOVA, Tukey test, Internal Preference Mapping (MDPREF), Cluster analysis, Principal Component Analysis (PCA), Partial Least Square regression (PLS), and Extended Internal Preference Map (EPM). In general wines produced from Ives (V. labrusca) grape showed higher aroma/flavor notes described as sweet, grape, grape juice, blackberry and roses. The wines produced from the hybrid grape Máximo differed from those elaborated with the variety Ives, especially due to their higher intensity of earthy/mushroom, vegetative/green beans, woody and yeast sensory notes. The PLS and EPM analyses indicated that fruity notes associated with the aroma and flavor of grape and grape juice were sensory drivers of Brazilian consumers' preference. On the other hand, the majority of the consumers did not like the sensory notes described as earthy/mushroom, vegetative/green beans and yeast found in the wines produced from Máximo. The wine elaborated exclusively with the hybrid Seibel 2 and the wine containing the V. labrusca grapes Ives and Isabella were preferred by the majority of the consumers and only a minor segment of consumers appreciated the wines elaborated solely with Máximo grape. Thus the hybrid grape Seibel 2 and the recently developed hybrid Máximo were shown to be promising varieties for the winemaking of quality wines in Brazilian regions where the environmental conditions are not good for the cultivation of V. vinifera varieties.

Exogenous Abscisic Acid Increases the Anthocyanin Concentration of Berry and Juice from ‘Isabel’ Grapes (Vitis labrusca L.)

Vitis labrusca grapes are widely cultivated in some countries such as Brazil and the United States, especially for juice production. ‘Isabel’ is one of the main cultivars because of its high production and disease tolerance, but the juice is deficient in color. The objective of this study was to evaluate the effect of timing and concentration of (S)-cis-abscisic acid (S-ABA), a specific isomer of abscisic acid applied to ‘Isabel’ grapes, aiming to increase anthocyanin accumulation and to improve juice quality. The trial was conducted on two consecutive seasons (2011 and 2012) in a commercial vineyard located in Maringa, PR, Brazil. The vines were trained in an overhead trellis system and spaced at 4.0 × 1.0 m. The statistical model used was a randomized block design with four replications and five treatments: control; S-ABA at 200 mg·L−1 at 7 days after veraison (DAV); S-ABA at 400 mg·L−1 at 7 DAV; S-ABA at 200 mg·L−1 at 7 DAV + 200 mg·L−1 at 15 days after the first application (DAFA); and S-ABA at 400 mg·L−1 at 7 DAV + 400 mg·L−1 at 15 DAFA. There were no significant differences between treatments in relation to berry or cluster size or weight. S-ABA application resulted in increased anthocyanin concentrations in both berries and juices, mainly when 400 mg·L−1 was applied. Sensory analysis also revealed the efficiency of this plant growth regulator to promote better acceptance of grape juices. Thus, a single application of S-ABA at 400 mg·L−1 at 7 DAV is sufficient to increase the anthocyanins concentration of ‘Isabel’ grapes, improving the acceptance of juice by consumers.

Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis labrusca grapes: Evaluation of different extraction methods

Polyphenols have been associated with the bioactive potential of grapes due to their beneficial health effects. For this reason, the aim of this study was to determine the content of bioactive compounds and in vitro antioxidant activity of selected Vitis vinifera and Vitis labrusca grapes widely produced in Brazil. In order to evaluate the efficiency of different methods for the extraction of the main polyphenols in grape, four methods were tested and compared. Among the extraction methods evaluated, liquid–liquid extraction was selected as the best method, which showed the best precision and repeatability. The phenolic compounds of white and red V. vinifera and V. labrusca grapes indicated that the type and concentration of polyphenols were mainly dependent on the grape variety rather than the species. The antioxidant activity of all grape samples evaluated was strongly correlated to the trans-resveratrol content (R>0.9). Among all samples, it was observed that V. labrusca varieties, especially Bordô and Concord grapes widely cultivated in the south of Brazil, presented higher antioxidant potential, associated with the presence of higher content of bioactive compounds.

Anti-Atherogenic Effects of a Phenol-Rich Fraction from Brazilian Red Wine (Vitis labrusca L.) in Hypercholesterolemic Low-Density Lipoprotein Receptor Knockout Mice

Moderate wine intake (i.e., 1-2 glasses of wine a day) is associated with a reduced risk of morbidity and mortality from cardiovascular disease. The aim of this study was to evaluate the anti-atherosclerotic effects of a nonalcoholic ethyl acetate fraction (EAF) from a South Brazilian red wine obtained from Vitis labrusca grapes. Experiments were carried out on low-density lipoprotein (LDL) receptor knockout (LDLr⁻/⁻) mice, which were subjected to a hypercholesterolemic diet and treated with doses of EAF (3, 10, and 30 mg/kg) for 12 weeks. At the end of the treatment, the level of plasma lipids, the vascular reactivity, and the atherosclerotic lesions were evaluated. Our results demonstrated that the treatment with EAF at 3 mg/kg significantly decreased total cholesterol, triglycerides, and LDL plus very low-density lipoprotein levels compared with control hypercholesterolemic mice. The treatment of mice with EAF at 3 mg/kg also preserved the vasodilatation induced by acetylcholine on isolated thoracic aorta from hypercholesterolemic LDLr⁻/⁻ mice. This result is in agreement with the degree of lipid deposit on arteries. Taken together, the results show for the first time that the lowest concentration of an EAF obtained from a red wine produced in southern Brazil significantly reduced the progression of atherosclerosis in mice.

Comparison of Odor-Active Compounds in Grapes and Wines from Vitis vinifera and Non-Foxy American Grape Species

Native American grape (Vitis) species have many desirable properties for winegrape breeding, but hybrids of these non-vinifera wild grapes with Vitis vinifera often have undesirable aromas. Other than the foxy-smelling compounds in Vitis labrusca and Vitis rotundifolia , the aromas inherent to American Vitis species are not well characterized. In this paper, the key odorants in wine produced from the American grape species Vitis riparia and Vitis cinerea were characterized in comparison to wine produced from European winegrapes (V. vinifera). Volatile compounds were extracted by solid-phase microextraction (SPME) and identified by gas chromatography-olfactometry/mass spectrometry (GC-O/MS). On the basis of flavor dilution values, most grape-derived compounds with fruity and floral aromas were at similar potency, but non-vinifera wines had higher concentrations of odorants with vegetative and earthy aromas: eugenol, cis-3-hexenol, 1,8-cineole, 3-isobutyl-2-methoxypyrazine (IBMP), and 3-isopropyl-2-methoxypyrazine (IPMP). Elevated concentrations of these compounds in non-vinifera wines were confirmed by quantitative GC-MS. Concentrations of IBMP and IPMP were well above sensory threshold in both non-vinifera wines. In a follow-up study, IBMP and IPMP were surveyed in 31 accessions of V. riparia, V. rupestris, and V. cinerea. Some accessions had concentrations of >350 pg/g IBMP or >30 pg/g IPMP, well above concentrations reported in previous studies of harvest-ripe vinifera grapes. Methyl anthranilate and 2-aminoacetophenone, key odorants responsible for the foxiness of V. labrusca grapes, were undetectable in both the V. riparia and V. cinerea wines (<10 μg/L).