Laboratory-based Methodology Research Articles

Antibiotic resistance patterns in human, animal, food and environmental isolates in Ghana: a review.

Many articles have been published on resistant microorganisms isolated from humans, animals, foods and the environment in Ghana. However, there are no reviews that summarize the information on the isolates and antibiotics tested so far in the country. This literature review was completed through “PubMed” and “Google Scholar” searches. We included publications from the period 1975-2015 with a laboratory-based methodology to determine antibiotic resistance of strains isolated in Ghana. In total, 60 articles were included in the analysis with 10% of the articles carrying out nationwide research on antibiotic resistance. The regions of Ghana with the highest published articles were Greater Accra (40%), Ashanti (21.7%) and Northern Region (10%). Most of the studies (86.7%) were related to isolates collected from human samples followed by environmental (5%), animal (3%) and food samples (2%). Ten different bacteria genera were observed in the studies. The most common was Escherichia coli, followed by Staphylococcus spp., Mycobacterium spp. and Streptococcus spp. The highest mean resistance rate was encountered in Escherichia coli (62.2%) followed by Klebsiella spp. (60.4%) and Pseudomonas spp. (52.1%). High resistance rates have been found in Ghana, however, the data are skewed and some regions of the country have been neglected. There is a need for higher quality research to establish and monitor resistance patterns in Upper West, Brong-Ahafo, Volta and Eastern Regions of Ghana.

Cultivating creative workplaces through mindfulness

Purpose The purpose of this paper is to examine how mindfulness training impacts creativity with individuals in a workgroup and propose a methodology for future research. Design/methodology/approach The methodology developed drew on existing laboratory-based research and applied those designs in a real-world application. The sample participants were from a mid-sized real estate firm that included ten realtors and support staff, six in the treatment group and four in the comparison group. The study took place over 16 weeks where pre-test and post-test mindfulness and creativity assessments were administered. A five-week mindfulness training was conducted with the treatment group and following the post-tests with the comparison group. Findings Results indicated that the mindfulness training positively impacted creativity in the moment and over time. There was evidence that the mindfulness training positively impacted an individual’s level of attention and awareness in daily activities which is likely to influence creative outcomes in organizational settings. Research limitations/implications This study shows that it is possible to design experimental studies in work settings and contribute to the empirical research about mindfulness despite the widely held perception about scarcity of time and lack of access to do such research. The findings also build on existing literature and address some of the gaps in current research. The most notable limitation relates to the small sample size. Practical implications The finding affirms that even a short but consistent practice of mindfulness in organizations can lead to a measurable increase in creativity. Originality/value This empirical study adds value to existing literature by expanding laboratory-based methodology to a practical application. One of the unique aspects of this research relates to the sample population. This research was conducted with an intact workgroup and translates the insights gained from laboratory research to a potential benefit for an organization by applying a version of this methodology to enhance its workgroup creativity.

Pediatric laboratory-based screening methodology for nutrition-based disorders

Pediatric laboratory-based screening methodology for nutrition-based disorders

An integrated approach for assessing the bioreceptivity of glazed tiles to phototrophic microorganisms

A laboratory-based methodology was designed to assess the bioreceptivity of glazed tiles. The experimental set-up consisted of multiple steps: manufacturing of pristine and artificially aged glazed tiles, enrichment of phototrophic microorganisms, inoculation of phototrophs on glazed tiles, incubation under optimal conditions and quantification of biomass. In addition, tile intrinsic properties were assessed to determine which material properties contributed to tile bioreceptivity. Biofilm growth and biomass were appraised by digital image analysis, colorimetry and chlorophyll a analysis. SEM, micro-Raman and micro-particle induced X-ray emission analyses were carried out to investigate the biodeteriorating potential of phototrophic microorganisms on the glazed tiles. This practical and multidisciplinary approach showed that the accelerated colonization conditions allowed different types of tile bioreceptivity to be distinguished and to be related to precise characteristics of the material. Aged tiles showed higher bioreceptivity than pristine tiles due to their higher capillarity and permeability. Moreover, biophysical deterioration caused by chasmoendolithic growth was observed on colonized tile surfaces.

Training on uncertain ground: Farma bez jeskyně/Farm without a cave

The recipient of numerous honours including Edinburgh Fringe First, Total Theatre and Herald Angel Awards and the European Prize for New Theatrical Realities (2011), Farma v jeskyni is among the most successful theatre companies to emerge from the Czech new theatre (nové divadlo) scene. Despite, or perhaps because of, its success, Farma's reception domestically is fraught with ambiguity and a suspicion of the company's laboratory-based methodology, which emphasises the daily physical training of company members. This article considers Farma's work and its domestic critical reception and interrogates the disparity between the company's international renown and compromised working conditions in Prague. It demonstrates the extent to which Farma's reception and treatment is shaped by the artistic values and priorities established throughout the historical evolution of Czech theatre as a highly politicised and national art form. Farma's emphasis on training and ensemble practice will be explored as inflecting the company's critical perception and working conditions, with reference to communist-era scepticism concerning Grotowski's actor-training practices, as well as post-communist neo-liberal agendas that emphasise saleable cultural products over artistically rigorous processes.

Sand column impact onto a Kolsky pressure bar

A laboratory-based methodology to launch cylindrical sand slugs at high velocities is developed. The methodology generates well-characterised soil ejecta without the need for detonation of an explosive; this laboratory-based tool thereby allows for the experimental investigation of the soil-structure events. The experimental set-up comprises a launcher with a cylindrical cavity and a piston to push out the sand slug. The apparatus is used to launch both dry and water-saturated sand slugs. High speed photography is used to characterise the evolution of the sand slugs after launch. We find that the diameter of the slugs remains unchanged, and the sand particles possess only an axial component of velocity. However, the sand particles have a uniform spatial gradient of axial velocity and this results in lengthening of the slugs as they travel towards their target. Thus, the density of the sand slugs remains spatially homogenous but decreases with increasing time. The velocity gradient is typically higher in the dry sand slugs than that of the water-saturated slugs. The pressure exerted by the slugs on a rigid-stationary target is measured by impacting the slugs against a direct impact Kolsky bar. After an initial high transient pressure, the pressure reduces to a value of approximately ρv2 where ρ is the density of the impacting sand slug and v is the particle velocity. This indicates that loading due to the sand is primarily inertial in nature. The momentum transmitted to the Kolsky bar was approximately equal to the incident momentum of the sand slugs, regardless of whether they are dry or water-saturated.

An Immunologic Model for Rapid Vaccine Assessment — A Clinical Trial in a Test Tube

While the duration and size of human clinical trials may be difficult to reduce, there are several parameters in pre-clinical vaccine development that may be possible to further optimise. By increasing the accuracy of the models used for pre-clinical vaccine testing, it should be possible to increase the probability that any particular vaccine candidate will be successful in human trials. In addition, an improved model will allow the collection of increasingly more-informative data in pre-clinical tests, thus aiding the rational design and formulation of candidates entered into clinical evaluation. An acceleration and increase in sophistication of pre-clinical vaccine development will thus require the advent of more physiologically-accurate models of the human immune system, coupled with substantial advances in the mechanistic understanding of vaccine efficacy, achieved by using this model. We believe the best viable option available is to use human cells and/or tissues in a functional in vitro model of human physiology. Not only will this more accurately model human diseases, it will also eliminate any ethical, moral and scientific issues involved with use of live humans and animals. An in vitro model, termed "MIMIC" (Modular IMmune In vitro Construct), was designed and developed to reflect the human immune system in a well-based format. The MIMIC System is a laboratory-based methodology that replicates the human immune system response. It is highly automated, and can be used to simulate a clinical trial for a diverse population, without putting human subjects at risk. The MIMIC System uses the circulating immune cells of individual donors to recapitulate each individual human immune response by maintaining the autonomy of the donor. Thus, an in vitro test system has been created that is functionally equivalent to the donor's own immune system and is designed to respond in a similar manner to the in vivo response.

The effects of PTSD symptomatology on laboratory‐based aggression

The present study sought to examine the relationship between posttraumatic stress disorder (PTSD) symptomatology and aggression using a highly controlled, quasi-experimental laboratory-based aggression paradigm among a sample of 85 undergraduate students not help-seeking for current PTSD phenomena. Congruent with theory and past research, results showed that PTSD symptomatology was positively associated with aggression. In demonstrating this effect using a laboratory-based methodology, the present study provides a heuristic for future researchers to explore potential mediators and moderators of the PTSD-aggression relationship.

Longer Exposure Durations Increase Freeze Damage to Turf Bermudagrasses

Bermudagrasses, Cynodon sp, are susceptible to winter injury in the transition zone for warm- and cool-season turfgrasses. Our objectives were to determine relative freeze tolerance of recently released and standard cultivars using laboratory-based methodology, and to determine the effect of extended exposure duration on survival. Plants were clonally propagated, then established and acclimated in growth chambers before exposure to a range of temperatures in a freeze chamber. ‘Tifway’ and ‘TifSport’ (−7.9°C) were significantly hardier than ‘Princess’ (−6.9°C), but less freeze tolerant than ‘U-3’ (−8.9°C), ‘Patriot’ (−9.7°C), and ‘Midlawn’ (−10.3°C). Riviera (−8.3°C) was significantly hardier than Princess, but less freeze tolerant than Patriot and Midlawn. In a second set of experiments, acclimated plants were held at constant, subfreezing temperatures for various periods of time in a refrigerated bath. Survival of U-3 and Riviera decreased to 25% or less when exposed to −7.0°C for 2 or 5 d, compared with 100 and 83% survival, respectively, when plants were removed from the bath immediately after equilibrating at −7.0°C. Princess exhibited 89% survival when removed immediately after equilibrating at −5.4°C, but survival after 2, 24, and 72 h was 67, 30, and 11%, respectively. Although minimum exposure temperature is a primary determinant of survival, freeze damage to turf bermudagrasses increased as exposure duration increased.

Numerical Modeling of Scour in Cohesive Soils Around Artificial Rock Island of Cooper River Bridge

A new laboratory-based methodology for prediction of the maximum scour depth in cohesive soil has recently been developed at the University of South Carolina. Because of the absence of field data, a computational fluid dynamics model, FLUENT, is used to test the scale effects associated with such a methodology. The numerical model was first verified against measurements obtained in the laboratory. The numerical results agreed satisfactorily with the measurements. Then, the numerical model was applied to the rock island protecting the main piers of the Cooper River Bridge, located in Charleston, South Carolina. The scour hole created around the island in the laboratory was scaled up and used in the numerical model. The computed bed shear stresses compared satisfactorily with those scaled up from the measurements and the shear stress at which the field sample begins to erode. It was found that the scour depth of 3.7 m represents the equilibrium state, which is similar to the results scaled up from the laboratory experiments. The numerical results showed that the scour depth of 36 m calculated by the HEC-18 approach is significantly overestimated.

Toxicologic aspects of heroin substitution treatment.

Heroin abuse is an international problem with which all countries must continually cope. Many countries have implemented heroin substitution therapy as an effective means of decreasing illicit heroin use, crime, HIV risk, and death, and in improving employment and social adjustment. Although methadone is the most commonly used medication for heroin substitution, other agonists in current use include levomethadyl acetate (LAAM), buprenorphine, and pharmaceutical-grade heroin. This report reviews toxicologic issues that arise in these programs. A broad array of testing methodologies are available that allow selection of on-site testing or laboratory-based methodology. Urine specimens may be monitored for nonprescribed drugs on a qualitative or semiquantitative basis. Methods for differentiating opiate sources by urinalysis have been proposed to distinguish poppy seed consumption from heroin abuse and for distinguishing pharmaceutical-grade heroin from illicit heroin. Therapeutic drug monitoring for methadone in plasma continues to be evaluated for use in establishing adequate dosing and detecting diversion, and new methods have been devised for measurement of the optical isomers of methadone in plasma. Biologic specimens, in addition to plasma and urine, have been evaluated for use in drug monitoring, including sweat, hair, and oral fluid, with promising results. Overall, the many recent developments in testing methodology provide more effective means to assess patients in heroin substitution programs and should contribute to improvements in public health.

Freeze tolerance of bermudagrasses

Bermudagrasses, Cynodon spp., periodically sustain winter injury in the transition zone between warm- and cool-season turfgrasses. Our objective was to determine relative freeze tolerance levels of advanced lines, recently released cultivars, and standard varieties by means of laboratory-based methodology. Freeze tolerance evaluations were divided into three groups on the basis of intended use. The vegetatively propagated fairway types (and their freeze tolerance values) included ‘GN-1’ (−5.9°C), ‘Baby’ (−6.7°C), ‘Tifway’ (−6.7°C), ‘TifSport’ (−7.2°C), ‘Quickstand’ (−8.0°C), and ‘Midlawn’(−8.4°C). GN-1 was significantly less hardy than TifSport, Quickstand and Midlawn. The second set of bermudagrasses comprised the seed-propagated varieties: ‘Arizona Common’ (−5.6°C), ‘Mirage’ (−6.1°C), ‘Jackpot’ (−6.3°C), ‘Guymon’ (−7.4°C), and ‘Yukon’ (−7.6°C). Arizona Common was significantly less freeze tolerant than Guymon and Yukon. Mirage and Jackpot were not significantly hardier than Arizona Common. The third series of plants included vegetatively propagated bermudagrasses used for putting greens: ‘Champion’ (−4.8°C), ‘Floradwarf’ (−4.9°C), ‘MS-Supreme’ (−5.2°C), ‘MiniVerde’(−5.8°C), ‘Tifeagle’ (−6.0°C), ‘Tifdwarf’ (−6.5°C), and ‘Tifgreen’ (−6.5°C). Tifdwarf and Tifgreen were significantly hardier than all of the other putting green bermudagrasses tested except for Tifeagle. Results should be useful in selecting appropriate genotypes for the transition zone of turfgrass adaptation.

Determining Legibility Distance for Highway Signs: Is the within Subject Variability Being Overlooked?

Laboratory studies of warning symbol signs have been shown to underestimate legibility distances by up to a factor of two when compared with field studies. However, this research suggests it is more than simply experimental setting contributing to disparity in research findings. Using a group of old and young drivers, six symbol signs were investigated in both settings. With six trials per sign, legibility distances, defined as the distance at which the sign is correctly identified from a menu, were collected. Large within subject variability was discovered in both age groups. This variability led to alternative ways of defining the dependent variable equivalent to designs of past studies examining legibility distances of the same signs. Different results arose out of the subsets created. The consideration is not just should a field-based versus laboratory-based methodology be used. An argument is posed that recommended distances at which signs are placed must be determined from a “worst-case” scenario. This premise requires a reexamination of our research methodologies for determining placement of highway signs.