Kochi Prefecture Research Articles

(E)-7-Phenyl-2-heptene-4,6-diyn-1-ol from Bidens pilosa as a repellent against isopods.

The development of repellents as alternatives to insecticides has expanded in recent years. However, their use in isopod pest control is limited. To develop an isopod repellent, a plant extract library from wild plants native to the Kochi Prefecture was screened for repellent activity against pillbugs and 82 samples (87%) exhibited repellent activity. Among them, (E)-7-phenyl-2-heptene-4,6-diyn-1-ol was isolated and identified as a repellent from the root of Bidens pilosa. It had a half-maximal effective concentration of 0.20 µM, with a strong repellency. A study of the structure-activity relationship to (E)-7-phenyl-2-heptene-4,6-diyn-1-ol revealed that the presence of a hydroxyl group and an aromatic at both ends of the length of the seven-carbon chain is important for the expression of repellency. These results can potentially lead to a new repellent of phenylalkyl alcohol.

The First Record of <i>Ophioteichus multispinum</i> (Echinodermata: Ophiuroidea) from Japan, with Notes on Its Ossicle Description and Ecology

Three specimens of Ophioteichus multispinum H. L. Clark, 1938 are redescribed from approximately 2.3–12.6 m water depth at Sabiura, Kushimoto, Wakayama Prefecture in central Japan. The species occurs completely buried on sandy bottoms. Ossicle morphology is described. Based strictly on images, we present in situ occurrence of the genus Ophioteichus H. L. Clark, 1938 from the intertidal regions of Susami, Wakayama Prefecture, and Tosashimizu, Kochi Prefecture.

Japanese Encephalitis Virus Genotypes 1 and 3 Isolation in Kochi, Japan.

Japanese encephalitis virus (JEV) is a mosquito-borne virus belonging to the JEV serocomplex within the genus Flavivirus, family Flaviviridae. It has 5 genotypes, G1-G5, based on the envelope (E) protein nucleotide sequence. JEV G3 circulated in Japan until the early 1990s when it was replaced by G1. JEV G3 was isolated from swine serum samples (sw/Kochi/1/2004) in the Kochi Prefecture, western Japan, in 2004. In addition, the 2018 isolates from pigs and cows (sw/Kochi/492/2018 and bo/Kochi/211/2018) in the same prefecture were identified as G3. The nucleotide sequencing results of the sw/Kochi/492/2018 and bo/Kochi/211/2018 polyprotein region differed from those of the sw/Kochi/1/2004 strain described in our previous report. Seven JEV isolates were identified as G1 in the same geographical area as that in this study. This result indicates that both JEV G1 and G3 are present in the Kochi area.

External Validation of the Japanese Clinical Score for Mortality Prediction in Patients With Acute Heart Failure.

To predict mortality in patients with acute heart failure (AHF), we created and validated an internal clinical risk score, the KICKOFF score, which takes physical and social aspects, in addition to clinical aspects, into account. In this study, we validated the prediction model externally in a different geographic area.Methods and Results: There were 2 prospective multicenter cohorts (1,117 patients in Osaka Prefecture [KICKOFF registry]; 737 patients in Kochi Prefecture [Kochi YOSACOI study]) that had complete datasets for calculation of the KICKOFF score, which was developed by machine learning incorporating physical and social factors. The outcome measure was all-cause death over a 2-year period. Patients were separated into 3 groups: low risk (scores 0-6), moderate risk (scores 7-11), and high risk (scores 12-19). Kaplan-Meier curves clearly showed the score's propensity to predict all-cause death, which rose independently in higher-risk groups (P<0.001) in both cohorts. After 2 years, the cumulative incidence of all-cause death was similar in the KICKOFF registry and Kochi YOSACOI study for the low-risk (4.4% vs. 5.3%, respectively), moderate-risk (25.3% vs. 22.3%, respectively), and high-risk (68.1% vs. 58.5%, respectively) groups. The unique prediction score may be used in different geographic areas in Japan. The score may help doctors estimate the risk of AHF mortality, and provide information for decisions regarding heart failure treatment.

Factors Related to Nutritional Status of Single Older Residents in Semi-Mountainous Rural Regions of Japan: A Cross-Sectional Study.

Japan's notably high aging rate presents the risk of malnutrition. This study aimed to clarify the nutritional status and factors related to the nutritional status of single older residents in a semi-mountainous rural region of Japan. Using a cross-sectional study design, surveys were administered to older adults in the semi-mountainous rural region in the area of Kochi Prefecture, Japan. Factors associated with a risk of malnutrition were identified using binomial logistic regression analysis. In addition, nutritional status was evaluated using the Mini Nutritional Assessment-Short Form (MNA-SF). Among 53 participants, the MNA-SF score was 12.1 ± 1.5 (mean ± standard deviation), and 71.7% had a normal nutritional status. We observed that participation in local residents' association gatherings (odds ratio [OR]: 7.42, 95% confidence interval [CI]: 1.17-47.01) and risk of depression/anxiety (OR: 12.77, 95% CI: 1.99-81.94) were associated with an increased risk of malnutrition, whereas social interaction with friends (OR: 0.11, 95% CI: 0.02-0.76) were associated with a decreased risk. The nutritional status was normal overall. Community health workers should share information on the health of residents and promote social events to enable older residents living alone to continue leading healthy lifestyles.

Life‐cycle risk assessment of building portfolios subjected to tsunamis under non‐stationary sea‐level rise based on a compound renewal process

AbstractThe immense impacts of tsunamis can inflict substantial damage on coastal infrastructure systems during their lifetime and lead to considerable economic loss. However, with the increasing intensity and variability of sea‐level rise due to climate change, evaluations of the life‐cycle tsunami risk associated with cumulative loss have become increasingly complex since tsunami hazards are time‐dependent. In addition, the number of earthquake events and the corresponding arrival times are substantially uncertain. Therefore, an accurate life‐cycle tsunami risk assessment methodology should be established to appropriately develop disaster mitigation measures. This paper provides a novel life‐cycle risk assessment of building portfolios subjected to tsunami hazards under non‐stationary sea‐level rise effects due to climate change. The cumulative loss of a building portfolio is evaluated through a compound renewal process based on earthquake interarrival time uncertainties and time‐dependent risk. The earthquake interarrival times are modeled using a non‐Poisson process based on historical data. Tsunami hazard curves that consider the effects of sea‐level rise, estimated based on climate models, are obtained with tsunami propagation analysis. The time‐variant annual risk is estimated based on reliability and building unit loss. Finally, a numerical procedure is proposed to estimate the life‐cycle tsunami risk of building portfolios. An illustrative example is provided by applying the framework to several municipalities in the Kochi Prefecture of Japan to assess the effects of climate change on the life‐cycle tsunami risk.

Nondestructive characterization of diseased Chinese chive leaves using X-ray intensity ratios with microbeam synchrotron radiation X-ray fluorescence spectrometry.

The Chinese chive (Allium tuberosum) is a core crop grown in Kochi Prefecture, Japan. However, withering symptoms occur during greenhouse growing, which have a negative impact on crop management Chinese chive leaves with physiological disorders (PD) or necrotic streak disease (ND) present with withering as typical blight symptoms. Excess or deficiency of elements may cause such withering in Chinese chive leaves with PD. Therefore, visualizing the elemental distribution in plant bodies may help clarify the cause of this withering. In this study, using synchrotron radiation X-ray fluorescence (SR-XRF) imaging, we examined the elemental distribution conditions in healthy Chinese chive leaves without withering, those that withered due to PD, and those that withered due to ND. Segmentation analysis of inductively coupled plasma-optical emission spectroscopy (ICP-OES) was performed on the SR-XRF imaged Chinese chive leaves and the data from the two analytical methods were compared. SR-XRF imaging provided more detailed data on elemental distribution compared with segmentation analysis using ICP-OES. Based on the SR-XRF imaging results, the X-ray intensity ratios for Ca/K, Fe/Mn, and Zn/Cu were calculated. These findings support that the Ca/K, Fe/Mn, and Zn/Cu X-ray intensity ratios can be used in the early detection of withered leaves and to predict the factors causing withering.

Inclinometer and Improved SBAS Methods with a Random Forest for Monitoring Landslides and Anchor Degradation in Otoyo Town, Japan

Kochi Prefecture is located in an active zone of Japan that is frequently subjected to landslides due to heavy precipitation in typhoon seasons. Slow-moving landslides have been reported by both the local prefectural authorities and the National Government of Japan. We observed landslide movements in Otoyo Town by using ground- and satellite-based tools. Despite the high cost of establishing a borehole inclinometer survey to obtain accurate ground-based measurements, no previous InSAR study has been conducted in Otoyo Town, and the capacity for regional discrimination between active and inactive slow-moving landslides when using these tools remains unclear. We found that the horizontal velocity component was dominant at a rate of 21.4 mm/year across the whole of Otoyo Town. Satellite-based monitoring of ground-anchor efficiency may be possible in combination with ground-based inclinometer surveys. Three types of land cover are present in the study area—urban, field, and forests—and we selected a random forest (RF) model to extract low-coherence pixels by using optical and radar satellite sensors to identify important features and precisely remove pixels causing decorrelation. Long-term monitoring results from ground-based surveys, including inclinometer (boreholes) and anchor tension distribution data, were compared with the results of synthetic radar by using coherence-based small baseline subset (CB-SBAS) measurements. Generally, landslide occurrence was investigated across the whole of Otoyo Town, and we specifically evaluated the reliability of InSAR measurements in the Kawai landslide as a study site scale. The activity of the Kawai landslide channel was evaluated with borehole inclinometer displacement measurements (15.46 mm) and an anchor pressure survey (736 kN) from 2016 to 2019, as well as the steady state of the area (1.7 mm for the borehole inclinometer and 175 kN for the anchor pressure measurements), although a high cumulative precipitation of 3520 mm was reached during 2020 due to the ground anchor efficiency, which showed a consistent tendency with respect to the InSAR displacement measurements (14 mm during 2018 and 2019 and 0.7 mm during 2020). This comparison showed a consistent time-series displacement correlation, which was strengthened after introducing the RF mask into the analysis procedure, as the RF model correction reduced the standard deviation from the line-of-sight (LoS) average velocity estimation by 1.9 mm/year. Our research will help mitigate landslide impacts in Otoyo Town and its surroundings.

Association between subjective degree of influence in class and thinness among adolescents in Japan.

Social status in school, measured by subjective degree of influence in class (DOI), may influence thinness among adolescents. This study examined the association between subjective degree of influence in class and thinness among Japanese adolescents. Data were obtained from the Kochi Child Health Impact of Living Difficulty (K-CHILD) study in 2016, which Was a population-based study targeting 5th, 8th and 11th grade adolescents living in Kochi Prefecture, Japan (N = 9,998). DOI was assessed by adolescents via questionnaire. Weight and height were given by caregivers for 5th grade adolescents, whilst they were self-reported for 8th and 11th grade adolescents. Collected data on weight and height were used to calculate body mass index z-scores of WHO standards. Models included grade, gender, number of friends, household income, location of school and depressive symptoms as covariates. The results showed that both high and low DOI were positively associated with thinness after adjustment for other individual covariates (high DOI, OR = 1.59, 95% CI 1.05-2.43; low DOI, OR = 2.04, 95% CI 1.36-3.06). Further stratification by gender revealed that low DOI was positively associated with thinness (OR = 2.14, 95% CI 1.34-3.44) among boys, but there was no association among girls. Both high and low DOI were associated with the risk of being thin in adolescents. Focusing on DOI for adolescents may be important to address thinness among adolescents. Further studies are needed to examine the causality between DOI and thinness in adolescents.

Complete genome sequence of a novel ophiovirus associated with chlorotic disease of pepper (Capsicum annuum L.) in Japan

In July 2018, pepper plants (Capsicum annuum L.) with chlorotic leaves and fruits were observed in Kochi prefecture, Japan. High-throughput sequencing (HTS) identified the possible presence of an ophiovirus-like virus possessing three RNA segments in a chlorotic leaf. Using Sanger sequencing with primers designed based on the HTS results and a different source of RNA from the one used for HTS, the complete nucleotide sequences of three RNA segments encoding four proteins on their complementary strand were determined. The amino acid sequences of these four proteins showed similarity to those of the RNA-dependent RNA polymerase, RNA-silencing suppressor protein, movement protein, and coat protein, respectively, of ophioviruses, which are negative-sense single-stranded RNA viruses. However, the coat protein amino acid sequence of the virus found on pepper plants was no more than 61.9% identical to those of any known ophioviruses, which is lower than the species demarcation threshold of 85 %. Hence, we suggest that this virus, which we have named "pepper chlorosis associated virus" (PepCaV) should be considered a member of a new species in the genus Ophiovirus, for which we propose the name "Ophiovirus capsici". The results of phylogenetic analysis using coat protein amino acid sequences of PepCaV and other ophioviruses also supported this conclusion. PepCaV RNA was found to have conserved nucleotide sequences at both the 5' and 3' termini of the different RNA segments, and the conserved terminal nucleotide sequences were predicted to form a self-complementary double-stranded region, resulting in a panhandle structure in each of the genomic RNAs.

(No.5) Wooden buildings in Kochi prefecture

(No.5) Wooden buildings in Kochi prefecture

Automatic Threshold Temperature Assessment for Sweet Peppers Detection using Far-Infrared Camera

Fruit detection is a critical function in the field of agricultural robotics. The researchers have used various methods to precisely pinpoint the location of the fruit. This study identified sweet peppers in Kochi Prefecture, Japan, using a thermal recognition-based method. The obtained images were assessed and then used as inputs for a machine-learning system based on supervised categorization by applying an image processing technique. By automatically detecting the threshold temperature range employing artificial intelligence, it is proposed that the estimation result can be enhanced to some degree. The algorithms Bagging and K-Nearest Neighbourhood (KNN) were employed for this estimation. The harmonic average of recall and precision, otherwise known as the F value, is shown to have been within 30 to 40 percent on most occasions. Additionally, in certain instances, the KNN outperformed the F-value. The F-value for bagging hardly ever rises above 70%, but with KNN, it occasionally rises beyond 80%. As a result, it can be said that the detection method for automatically determining the temperature threshold is efficient for detecting sweet peppers.

Goishi tea leaf powder affects lower body weight and visceral fat accumulation during energy restriction in obese rats.

Goishi tea is a unique Japanese post-fermented tea produced in Kochi prefecture. The aim of this study was to investigate whether the supplementation of energy-restricted diet with Goishi tea leaves affects body weight, visceral fat accumulation, and fecal lipids in diet-induced obese rats. 18 male Wistar rats were fed a high-fat diet for 12 weeks. Subsequently, the diet-induced obese rats were fed a low-energy diet containing 1% (G1 group) or 3% (G3 group) of Goishi tea leaf powder, or without any tea extracts (C group) for 4 weeks. After 4 weeks, body weight and body fat ratio were significantly lower in the G3 group than in the C group. Plasma insulin levels were significantly higher in the C group than in the G1 and G3 groups, whereas plasma leptin levels were significantly lower in the G3 group than in the C group. In addition, the lipid absorption rate was significantly lower in the G3 group than in the C and G1 groups. In conclusion, the administration of Goishi tea leaves under dietary restrictions might contribute to body weight reduction and inhibition of lipid absorption, as a diet therapy to help prevent obesity and metabolic syndrome. J. Med. Invest. 70 : 60-65, February, 2023.

Impact of school closures on pediatric ambulance transport in Japan.

After the coronavirus disease (COVID-19) pandemic, nationwide school closures were implemented in many countries. This study aimed to determine the impact of the school closures on pediatric ambulance transport, comparing the situation before and after the COVID-19 pandemic. This retrospective observational study was conducted using data from the Kochi-Iryo.net database. In Kochi prefecture, schools were closed from March 6 to May 24, 2020. Pediatric emergency transport during the school closure period in 2020 was compared with that in the same period in 2019 (before the COVID-19 pandemic) and in 2021 (when schools were not closed). Statistical analysis comprised χ2 tests with Bonferroni adjustments for multiple testing. To adjust for patient backgrounds, we also performed multiple logistic regression analyses for numbers of pediatric ambulance transports. The rate of pediatric ambulance transports was significantly lower (p=0.008) in 2020 (276; 3.97%) than in 2019 (391; 4.87%), but there was no significant difference (p=0.360) between 2019 (391; 4.87%) and 2021 (352; 4.56%). Multivariable analysis revealed similar trends (2019 vs. 2020: OR 0.86, 95% CI 0.73-1.00; 2019 vs. 2021: OR 0.96, 95% CI 0.82-1.11). Regarding the characteristics of pediatric ambulance transport journeys, there were no significant differences in 2019, 2020, and 2021 in terms of sex, severity, locations of ambulance stations, and disease classification. There was a decrease in pediatric ambulance transportation due to the public health interventions for COVID-19, including school closures. However, this decrease was not solely due to school closures.

Hydrocarbon fluid inclusions in authigenic quartz from the Torinosu Limestone at Sakawa town, Kochi Prefecture, Japan

Hydrocarbon fluid inclusions in authigenic quartz from the Jurassic Torinosu Limestone and associated sediment at Sakawa town, Japan, were studied by microthermometry, ultraviolet (UV) fluorescence, and Raman spectroscopy. The quartz contained abundant liquid hydrocarbon and subordinate vapor hydrocarbon fluid inclusions, and tiny solid inclusions of bitumen, calcite, and rarely pyrite. Aqueous fluid inclusions and aqueous components in hydrocarbon inclusions were absent. Under UV irradiation, most of the liquid inclusions from the limestone and associated sediment displayed intense whitish-blue and deep-blue luminescence, respectively. Further, these liquid inclusions were homogenized at around 24 °C and 53 °C, respectively. Small numbers of liquid inclusions from the associated sediments contained a liquefied gas-condensate. Raman measurements indicated the presence of aromatic compounds and the absence of H2O or CO2 molecules in the liquid and vapor inclusions.

The Efforts and Spread of Breast Awareness in Kochi Prefecture and Yamakawa Breast Clinic

The Efforts and Spread of Breast Awareness in Kochi Prefecture and Yamakawa Breast Clinic

Association between urinary zinc excretion and isoflavone-metabolizing enterotypes among Japanese females: a cross-sectional study.

Zinc absorption and competition among gut bacteria have been reported in animal studies. Thus, gut bacteria may modify zinc availability in humans. Metabolism of intestinal bacteria is known to be necessary for the activation of several phytoconstituents in the body. For example, equol, a typical substance of soybean isoflavone, is produced by intestinal bacteria metabolizing daidzein and the enterotype is one of distinct ones among Japanese population. The difference in the intestinal microflora can modify the bioavailability of zinc. In this study, we examined urinary zinc concentrations in adult female equol producers (EQPs). Urine samples from women participating in health examinations in Miyagi, Okinawa, Kyoto, Kochi, and Hokkaido prefectures were used; from total 17,484 samples, approximately 25 samples were randomly selected for each age group from 30 to 60 years per region (subsample: n = 520), and 520 samples with available urinary zinc concentration (determined by flame atomic absorption analysis) and enterobacterial type were analyzed. EQP was defined as log(equol/daidzein) ≥ -1.42, and urinary concentrations were corrected for creatinine concentration. Urinary zinc concentrations were compared by Student's t-test and multiple regression analyses. The geometric mean urinary zinc concentration (µg/g-Cr) was lower in EQP than in non-EQP (p = 0.0136 by t-test after logarithm transformation). On the other hand, there was no correlation between urinary zinc concentration with daidzein (r = -0.0495, P = 0.436) and equol concentrations (r = -0.0721, P = 0.256). There was a significant negative association between urinary zinc concentration and EQP (β = -0.392, P = 0.0311) after adjusting with other potential confounding variables, such as daidzein intake. The results suggest that gut bacteria that produce equol are involved in the metabolism of zinc. Based on previous studies, the bacteria that affect the metabolism of both substances are thought to be Enterococcus. Future studies are expected to identify specific intestinal bacteria for zinc availability and understand individual differences in the effects of micronutrients.

No moderating effect of coping skills on the association between bullying experience and self-esteem: Results from K-CHILD study.

Few studies have investigated the moderating effect of coping skills on the association between bullying experience and low self-esteem. The aim of this study was to examine whether coping skills have a moderating effect on the association between bullying experience and self-esteem among Japanese students. Data from the population-based Kochi Child Health Impact of Living Difficulty (K-CHILD) study conducted in 2016 were analyzed. Participants included fifth-and eighth-grade students living in Kochi Prefecture, Japan. A questionnaire for the students (n = 5,991) assessed the bullying experience, self-esteem (the Japanese Edition of the Harter's Perceived Competence Scale for Children), and coping skills that comprised six types (The shortened version of coping skills for elementary school children). Multivariate linear regression analyses were conducted to examine the association between bullying experience and self-esteem and then the moderating effects of six types of coping as interaction terms on the association were considered. Bullying experience was inversely associated with self-esteem. All six types of coping did not moderate the relationship between bullying experience and low self-esteem even after adjusting for cofounders (all P for interaction > 0.15). Coping skills did not moderate the association between bullying experience and self-esteem, suggesting that intervention to boost coping skills to mitigate the adverse effect of bullying experience may not be promising.

Draft Genome Sequence of Streptomyces sp. Strain G-5, Isolated from Marine Sponge, Kochi Prefecture, Japan

Actinomycetes isolated from the marine environment often require the presence of seawater for their growth and/or morphological development. Here, we report the isolation and genome sequencing of marine sponge-derived Streptomyces sp. strain G-5 with such a seawater requirement.

Current state of knowledge and information sharing among home healthcare professionals involved in heart failure management

BackgroundThe current state of knowledge related to disease management of heart failure (HF) and information coordination practices provided by non-physician healthcare professionals such as nurses and therapists working at home-visit nursing stations in Japan are not well known. MethodsA questionnaire survey of healthcare professionals working at home-visit nursing stations was conducted in Kochi Prefecture. Data collected from 151 nurses and therapists were analyzed. ResultsRegarding the basic characteristics of the respondents, the majority were in their 30s and 40s, and approximately 75 % were nurses. In terms of HF knowledge, 53.7 % of respondents said that they “knew” about the New York Heart Association classification. A total of 40.0 % of respondents said that they were “aware of the existence of the HF handbook”, and only 29.3 % of respondents said that they “knew” the classification of HF stages. When they were asked about their level of satisfaction related to all medical information provided by the hospital and hospital wards, no one was “very satisfied”, and the most common response (66.2 %) was “not very satisfied”. In the essential medical information that respondents wanted to obtain from hospitals and hospitals wards for managing HF patients at home, “medication at discharge”, “current medical history”, “fluid intake and restrictions”, “symptoms, signs, and response to exacerbation”, and “ideal body weight” were the top five contents. ConclusionIn the cross-sectional study targeted healthcare professionals working at home-visit nursing stations in Kochi Prefecture, the current state of knowledge related to HF and information coordination practices among healthcare professionals were not fully satisfactory in HF management. It is necessary to increase educational opportunities regarding HF for them and further promote information sharing.