Considerations and practical recommendations for identifying perimenopause in longitudinal research.

Dentofacial Malocclusion and Salivary Biomarkers in Asthmatic Patients: A Scoping Review.

Fathers are prone to suboptimal bonding with their preterm infants, but little is known about the roles that father and infant variables might play in the suboptimal bonding. This gap in knowledge limits the ability of nurses to identify and assist fathers at risk of suboptimal bonding. This study aims to identify (1) the bonding levels of fathers to their preterm infants hospitalized in neonatal intensive care units (NICUs) and (2) the association between father and infant variables and bonding of fathers to their preterm infants hospitalized in (NICUs). This is a secondary data analysis from a parent study in which we recruited 396 fathers using a cross-sectional quantitative design across United States. In the original study, we recruited 396 fathers of preterm infants over 5 months (September 2022 to January 2023). We used self-reported measures to collect data specific to fathers and infant variables and bonding using Paternal Postnatal Attachment Scale. In this sample, 73.12 ± 10.32 represents the average level of bonding for fathers of preterm infants. Additionally, low household income, previous preterm infants, and current multiple-birth infants were significantly associated with low bonding levels for fathers of preterm infants. Results indicate a necessity to further support fathers' needs to bond with preterm infants in the NICU. Specifically, nurses can screen to identify fathers who are at risk for low bonding and provide at-risk fathers with an adequate degree of support like referral to social support.

Metals are a major class of legacy pollutants that end up in marine ecosystems, posing a significant threat to marine biota, including sea anemones. The current review critically synthesises studies published over the last 50years on the uptake, tissue distribution, and biological effects of 20 metals across 18 sea anemone species in both field and laboratory settings, including interactions with climate change stressors (salinity and pH). Field studies have focused on bioaccumulation and report the high capacity of sea anemones to accumulate metals, mainly iron and barium, primarily in the pedal disk. Laboratory exposure studies reveal a dose- and time-dependent accumulation and highlight that symbionts take up and store essential metals (Cu, Fe, and Mn) due to their key biological roles. Available data point to Exaiptasia pallida as a promising model for metal ecotoxicology. Across studies, metals elicit alterations at molecular to behavioural/morphological levels, including transcriptome reprogramming, oxidative stress, and detoxification failures, as well as genotoxicity, cellular injury, immune dysfunction, metabolic and morphological disruption, reproductive impairment, and bleaching, which are generally amplified by climate change stressors. Ultimately, this review identifies key knowledge gaps and outlines future research directions on metal ecotoxicity in sea anemones. Collectively, these insights position sea anemones as informative sentinels of metal contamination in marine ecosystems.

The unique biogenesis pathway of extracellular vesicles in Hirudo nipponia salivary gland cells.

Knowledge Gaps in Iron Deficiency Anemia Management in Obstetrics and Gynaecology Residents.

Staff awareness and engagement with the Green Plan: A cross-sectional survey of one of the largest NHS trusts in the UK.

Agricultural HDPE pyrolysis for environmental management: Feedstock complexity, reaction dynamics, and circular resource recovery.

The American Society of Anesthesiologists Physical Status (ASA-PS) classification system is ubiquitous in perioperative medicine and research as a tool for preoperative patient risk stratification. Despite widespread clinical adoption as a predictor of perioperative outcomes, the ASA-PS system is inherently subjective, leading to considerable inter-rater variability. A comprehensive mapping of the literature examining the relationship between ASA-PS scores and patient outcomes is lacking. To systematically map the extent, range, and nature of peer-reviewed literature examining the relationship between the ASA-PS classification and patient outcomes, and to identify key characteristics, themes, and knowledge gaps in this evidence base. This scoping review will be conducted according to the Joanna Briggs Institute (JBI) methodological framework and reported using the Preferred Reporting Items for Systematic Review and Meta-Analysis extension for Scoping Reviews (PRISMA-ScR). The Population-Concept-Context (PCC) framework will guide eligibility assessment. A comprehensive search will be conducted across PubMed, EMBASE, Scopus, LILACS, and the Cochrane Central Register of Controlled Trials, with no language or date restrictions. Study selection will be performed independently and in duplicate by two reviewers in two stages (title/abstract screening, full-text review). If any discordance appears, a third reviewer verdict will be requested. Data will be extracted using a structured charting form and synthesized narratively. Any healthcare setting where an ASA-PS score is assigned prior to a procedure (inpatient hospital, ambulatory surgery center, outpatient clinic). Primary research designs, including randomized controlled trials, observational studies (cohort, case-control, cross-sectional, descriptive), and case reports will be eligible; review articles, editorials, letters to the editor, and commentaries will be excluded. The search will employ controlled vocabulary (MeSH terms) and free-text keywords including: "ASA score," "ASA Physical Status Classification System," "American Society of Anesthesiologists," in combination with outcome-related terms. Supplementary hand searching of reference lists and Google Scholar will be performed. Study characteristics (author, year, country, journal, design), population characteristics (sample size, age, comorbidity), context (clinical setting, specialty, procedure type, urgency), ASA score details, and outcome details (including statistical methods used to derive associations) will be extracted. A preliminary data charting form is provided in Appendix B. Narrative synthesis supported by descriptive statistics will map study characteristics, outcome categories, clinical contexts, study designs, and temporal and geographical distribution of research. No formal quality appraisal will be conducted. Ethics committee approval is not required for this protocol-based scoping review.

Nationwide expert survey on transfusion and coagulation management strategies for bleeding critically ill patients in China.

The United Nations (UN) has a target for universal contraception access by 2030. Youth (aged 15-29) still have limited contraception access and lower usage. A unified understanding of the barriers youth face in high-income countries (HIC) remains unclear. Synthesized evidence on youth contraception barriers across HIC to identify continued healthcare inaccessibility and knowledge gaps. A search strategy, including terms like "youth" and "barriers," was applied to three databases, identifying articles published between January 2013-September 2024. Primary peer-reviewed quantitative, qualitative, and mixed-methods studies were included if they focused on youth and contraception barriers. Following the Joanna Briggs Institute, articles were screened for inclusion, and data was extracted. Analyses included descriptive statistics and summarizing findings for quantitative and qualitative results. All articles were subjected to inductive and deductive content analysis to map barriers. Article quality was appraised by the Mixed Methods Appraisal Tool. A total of 41 articles were included, of which 88% were from the USA. Youth struggled to receive quality contraception care from multiple access points from health systems and youth perspectives. Barriers included youth minimal knowledge, poor approachability and care appropriateness, physical barriers, costs, stigma, confidentiality concerns, and service gatekeeping. Youth experiences varied by social identities with those from lower economic, rural, and of younger age facing more obstacles. Contraception was inaccessible for many. To meet UN targets, efforts need to address described barriers to ensure accessible and equitable contraception care that respects and supports youth's choices.

Temporal dynamics of sperm morphology in broiler breeders: Evidence of rapid non-linear patterns of decline.

Tolerance strategy of Chlorella sorokiniana to combined antibiotic stress revealed by transcriptomic analysis.

African Swine Fever (ASF) is an infectious reportable disease of swine that can have devastating consequences for pork producers and consumers. ASF virus can persist in either domestic or wild swine through several epidemiological cycles. This has led to a wide variety of epidemiological traits, making it challenging to plan effective surveillance and mitigation strategies. Here, we compile and analyze data from experimental infections of ASF virus variants from genotype II that have been reported in peer-reviewed publications. We provide descriptive summaries of the distributions of epidemiological parameters such as incubation period, infectious period, mortality rate, and R0 and develop predictive models that explain the variation in these quantities based on detection assays and other experimental design features. Our results provide a comprehensive perspective of estimates of epidemiological parameters for ASFV, allowing for increased transparency in accounting for parameter uncertainty in ASF preparedness modeling. Our meta-analysis also provides insight on knowledge gaps and study design issues that could be addressed by future experimentation.

Phosphorus-solubilizing microorganisms: Advances in nutrient uptake mechanisms, plant growth promotion, and sustainable agriculture.

Seed treatment technologies: Effects on physical, functional, and physiological seed quality.

This study assessed the prevalence and clinical characteristics of Behçet's disease (BD) in the Middle East and North Africa (MENA) region, providing updated pooled estimates and identifying gaps in current epidemiological knowledge. We conducted a systematic review and meta-analysis of studies reporting the prevalence, epidemiology, or clinical features of BD in the MENA region, published in PubMed, MEDLINE, and Web of Science databases from 2014 onward. The eligible studies had observational designs and diagnoses based on internationally validated criteria. The study quality was assessed using the AXIS and MINORS tools. Pooled prevalence estimates and clinical feature proportions were calculated with 95% confidence intervals (CI); heterogeneity was quantified using I2. Ten studies (sample size, 31-5218) from seven countries were included. The reported prevalence ranged from 0.06% in Turkey to 0.66% in Jordan. The pooled prevalence was 0.003 (300/100,000 population; 95% CI 0.001-0.005; p = 0.004) with a high heterogeneity (I2 = 87.3%). No significant sex difference was observed in disease risk (odds ratio 1.55, 95% CI 0.59-4.05; p = 0.374). The pooled prevalence of major clinical features showed oral ulcers, genital ulcers, ocular involvement, and positive pathergy tests in 98.7%, 73.7%, 36.6%, and 54.6% of patients, respectively. BD prevalence in the MENA region remains among the highest worldwide. Clinical manifestations are consistent with global patterns but show regional variability, highlighting the need for standardized reporting, consistent epidemiological methodologies, and targeted strategies to support early diagnosis and recognition of the disease.

Role of SlJAIB14 in tomato defense against cotton bollworm (Helicoverpa armigera).

Extraction techniques, structural features, biological functions and bibliometrics of Moringa oleifera Lam. polysaccharides: an updated review.

Mapping knowledge and conservation gaps in Atlantic nurse shark research: A global North-South perspective