Khorasan Province Research Articles

Susceptibility Status of Several Field-Collected German Cockroaches (Blattella germanica) to a Pyrethroid Insecticide and Molecular Detection of Knockdown Resistance (kdr).

High frequency of insecticide used to control German cockroaches may lead to insecticide resistance development. We aimed to compare the level of insecticide resistance and heterogeneity in insecticide resistance of field-collected German cockroaches in eight selected zones of Mashhad City, Razavi Khorasan Province, Iran. The present study was conducted from October 2019 to May 2021. Adult and nymphs of German cockroaches were collected from infested restaurants or hotels in eight zones of Mashhad City and then colonized in an insectarium. The cockroaches were subjected to bioassay against cypermethrin insecticide. In addition, the genomic DNA of each cockroach population was analyzed in the region where the kdr mutations reside in the German cockroach. The LT50 values against cypermethrin in the susceptible strain (SS) and eight field-collected strains so varied from 17.52 to 95.36 min. The resistance ratio of the strains was also different in response to the insecticide. The similarity of multiple sequence alignments at the amino acid level was 97.5%-100%. An exon 20 transversion mutation and a nonsynonymous substitution were found. The L1014F substitution was detected in 83.4% of the cockroach samples. The field-collected strains were resistant to cypermethrin at different levels. Furthermore, the molecular study confirms the heterogeneity in the level of resistance among eight strains. Therefore, eight strains might have a different history of insecticide treatment.

Data on four species of Longidorus Micoletzky, 1922 (Nematoda: Longidoridae) from southern and southeastern Iran, including description of a new species.

Four species of the genus Longidorus were recovered from southern (Bushehr province) and southeastern (Southern Khorasan province) Iran. The first species, L. paratabrizicus n. sp. represents a new member to the genus and is characterised by 4.8-5.6 mm long females with anteriorly flattened lip region separated from the rest of the body by depression, amphidial fovea pocket-shaped without lobes, tail conical, dorsally convex, ventrally almost straight with bluntly rounded tip and males in population. By having similar lip region and tail shape, the new species most closely resembles five species viz. L. artemisiae, L. globulicauda, L. patuxentensis, L. sturhani, and L. tabrizicus. It represents the cryptic form of the last species. The second species belongs to L. mirus, recovered in both southern and southeastern Iran, representing the first record of the species after its original description. As an update to the characteristics of this species, it's all juvenile developmental stages were recovered and described. The criteria to separate L. mirus from two closely related species, L. auratus and L. africanus, are discussed. The third species belongs to L. persicus, a new record in southern Iran. The fourth species, L. orientalis was recovered in high population density in association with date palm trees in Bushehr province. The phylogenetic relationships of the new species and recovered populations of L. mirus and L. persicus were reconstructed using two ribosomal markers and the resulted topologies were discussed.

Community responses during early phase of the COVID-19 epidemic in the Razavi Khorasan province of Iran: a cross-sectional study

Community responses during early phase of the COVID-19 epidemic in the Razavi Khorasan province of Iran: a cross-sectional study

Comparison of Two Methods, Gradient Boosting and Extreme Gradient Boosting to Pre- dict Survival in Covid-19 Data

Introduction: The present study discusses the importance of having a predictive method to determine the prognosis of patients with diseases like Covid-19. This method can assist physicians in making treatment decisions that improve survival rates and avoid unnecessary treatments. This research also highlights the importance of calibration, which is often overlooked in model evaluation. Without proper calibration, incorrect decisions can be made in disease treatment and preventive care. Therefore, the current study compares two highly accurate machine learning algorithms, Gradient boosting and Extreme gradient boosting, not only in terms of prediction accuracy but also in terms of model calibration and speed. Methods: This study involved analyzing data from Covid-19 patients who were admitted to two hospitals in Mashhad city, Razavi Khorasan province, over a span of 18 months. The k-fold cross-validation method was employed on the training dataset (K=5) to conduct the study. The accuracy and calibration of two methods (Gradient boosting and Extreme gradient boosting) in predicting survival were compared using the Concordance Index and calibration. Results: The Concordance Index values obtained for gradient boosting and Extreme gradient boosting models were 0.734 and 0.736, in the imbalanced and In the balanced data, the Concordance Index values were 0.893 for gradient boosting and 0.894 for Extreme gradient boosting. The surv.calib_beta index, the gradient boosting model had an estimated value of 0.59 in the imbalanced data and 0.66 in the balanced data. The Extreme gradient boosting model had an estimated value of 0.86 in the balanced data and 0.853 in the imbalanced data. The Extreme gradient boosting model was faster in the learning process compared to the gradient boosting model. Conclusion: The Gradient boosting and Extreme gradient boosting models exhibited similar prediction accuracy and discrimination power, but the Extreme gradient boosting model demonstrated relatively good calibration compare to Gradient boosting model.

Building readiness for climate change: A study of organizational learning in the management of natural resources, northeastern Iran

Readiness is critical to adaptation to climate change and includes how organizations adjust their structures, processes, and policies for both mitigation and adaptation. Organizational learning regarding climate change is the process by which organizations and institutions acquire the knowledge, skills, and capabilities necessary to understand, manage, and adapt to the impacts of climate change. This process emphasizes continuous learning, innovation, and collaboration as key strategies for effectively responding to the ongoing climate crisis. The aim of this study was to investigate the current state of organizational learning related to climate change among the General Directorates of Natural Resources and Watershed Management in Golestan and North Khorasan provinces of Iran. The research was conducted using a survey method, through which 80 experts from two organizations were randomly selected to complete a structured questionnaire. The findings revealed that although employees have a strong attitude towards climate change, organizational learning related to climate change is weak. The five dimensions of organizational learning (knowledge acquisition, information distribution, information interpretation, organizational memory, information application) were assessed. Information interpretation had the most weight, while organizational memory had the least. Positive attitudes toward climate change were associated with increased engagement in climate change-related organizational learning. The study recommends implementing training and awareness initiatives, fostering knowledge sharing and retention, encouraging positive attitudes towards climate change, and promoting collaboration to enhance organizational learning as a prerequisite for organizational readiness to climate change.

Isolation of two steroidal saponins with antileishmanial activity from Allium giganteum L.

Alliums are rich sources of steroidal saponins, flavonoids, and sulphoric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium giganteum (giant onion) which is named locally "Couria" in the Northeast of Iran, is grown widely in "Kouh-Sorkh" mountains in Khorasan province. Phytochemical investigation of chloroform-methanol and aqueous extract of the plant resulted in the isolation and identification of two steroidal saponins, using comprehensive spectroscopic methods including 1D and 2D NMR and MS. The chemical structures of the isolated saponins were determined as (22S)-cholesta-1b,3b,16b,22b-tetraol 5-en, and 3-O-β-D-glucopyranosyl26-O-β-D-glucopyranosside and (25R)-26-O-β-D-glucopyranosyl-5α-furostan-1α,3β,22α,26-tetraol3-O-{β-D-galactopyranosyl-(1→2)-O-[β-D-xylopyranosyl- (1→3)]-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside}. Investigation of in vitro antileishmanial activity of the isolated compounds at 10, 50, and 100 μg/mL exhibited significant leishmanicidal against the promastigotes of Leishmania major. The results established a valuable basis for further studies about A. giganteum and the anti-parasitic activity of steroidal saponins.

The Emergence of Linezolid-Resistant Staphylococcus Epidermidis in the COVID-19 Hospitalized Intubated Patients in North Khorasan, Iran.

The present study aimed to investigate secondary bacterial infections among patients infected with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Coagulase-negative Staphylococci can infect immunocompromised patients. Linezolid resistance among Staphylococcus epidermidis is one of the most critical issues. In 2019, 185 SARS-CoV-2-positive patients who were admitted to North Khorasan Province Hospital (Bojnurd, Iran), were investigated. Patients having positive SARS-CoV-2 reverse transcriptase real-time polymerase chain reaction (RT-PCR) test results, who had a history of intubation, mechanical ventilation, and were hospitalized for more than 48 hours were included. After microbiological evaluation of pulmonary samples, taken from intubated patients with clinical manifestation of pneumonia, co-infections were found in 11/185 patients (5.94%) with S. epidermidis, Staphylococcus aureus, and Acinetobacter baumani, respectively. Remarkably, seven out of nine S. epidermidis isolates were linezolid resistant. Selected isolates were characterized using antimicrobial resistance patterns and molecular methods, such as Staphylococcal cassette chromosome mec (SCCmec) typing, and gene detection for ica, methicillin resistance (mecA), vancomycin resistance (vanA), and chloramphenicol-florfenicol resistance (cfr) genes. All of the isolates were resistant to methicillin, and seven isolates were resistant to linezolid. Nine out of 11 isolated belonged to the SCCmec I, while two belonged to the SCCmec IV. It should be noted that all patients had the underlying disease, and six patients had already passed away. The increasing linezolid resistance in bacterial strains becomes a real threat to patients, and monitoring such infections, in conjunction with surveillance and infection prevention programs, is very critical for reducing the number of linezolid-resistant Staphylococcal strains. A preprint of this study was published at https://europepmc.org/article/ppr/ppr417742.

Evaluation of the Status of Knowledge, Attitude, and Performance of Radiology Department Staff Regarding Radiation Safety Principles at Hospitals in the North and Northeast of Iran

Purpose: Although ionizing radiation is useful in diagnosing various diseases, it can cause potential biological damage such as cancer, cataracts, and fetal damage for patients and staff working in radiology departments. Therefore, knowledge and practice about applying radiation protection principles are essential. This research investigates radiology personnel's knowledge, attitude, and performance regarding radiation protection in the north and northeast of Iran.
 Materials and Methods: This descriptive-analytical cross-sectional study was conducted using a 30-item questionnaire among 435 radiology personnel in North Khorasan, Razavi Khorasan, Golestan, and Mazandaran provinces. This questionnaire included questions related to demographic information and the level of knowledge, attitude, and performance of radiology personnel regarding radiation protection. Data was also analyzed using SPSS-19 software.
 Results: The participation rate of radiology personnel was 80.55%, and the mean and standard deviation of their knowledge, attitude, and performance regarding radiation protection were 45.9907 ± 1.294, 78.1531 ± 4.707, and 44.9368 ± 6.88, respectively. Based on the results of the study, there is no significant relationship between gender and knowledge (P = 0.781), attitude (P = 0.156), and performance of personnel (P = 0.87); however, a significant relationship was observed between education degree and attitude of personnel (P = 0.026), between working years and knowledge of personnel (P = 0.019), and also between job title and attitude of personnel (P = 0.003).
 Conclusion: The results of this study revealed that there is a significant relationship between education degree and attitude of personnel, working years, and knowledge of personnel, and also between job title and attitude of the personnel. According to these results, our population, both personnel and patients have relatively poor performance and poor knowledge about radiation safety principles. Therefore, formal training in the use of ionizing radiation equipment is necessary for radiation safety.

Some epidemiological feature of human visceral leishmaniasis in North Khorasan Province during 2010-2018

Some epidemiological feature of human visceral leishmaniasis in North Khorasan Province during 2010-2018

Systematic design of habitat services network (HSsN) for updating conservation areas in iran's arid and Semi-Arid ecosystems

Threats caused by human activities have reduced the quantity and quality of wildlife habitats connectivity. This research seeks to protect habitat biodiversity by creating a network of high quality habitats that remained. Therefore, it is very necessary to know about the dynamic effects of changes in the landscape pattern on the quality of the habitat, because it helps to preserve biodiversity, guarantee ecological security, and environmental sustainability. In the present study, a systematic process was used to design a network of habitat services comprising hotspots and corridors in arid and semi-arid ecosystems of eastern Iran, South Khorasan Province. In this process, habitat quality was first modeled using the InVEST software, then data mining methods were used to identify habitat hotspots and their spatial distribution. In the next step, habitat corridors were extracted based on the circuit theory and using the Circuitscape software. In the ultimate step, using graph theory based on the concept of ecological security, the connectivity of the habitat network was evaluated. The results of habitat network construction showed that 8% and 6% of the total area of the study area included key habitat patches and corridors, respectively. On the other hand, the overlapping of habitat network components with the conserved areas of the study area showed that free or non-conservation areas make up a significant amount of network components (49.18% of the habitat patch and 80.02% of the habitat corridor). These results reveal the existence of habitat protection potential and can help in updating the new boundaries of conservation areas. Also, the composite indices of dPC and dIIC could determine the importance of each of the network's components in evaluating the connectivity of the habitat network, so that the application of the results can show the priority options to the land managers for protecting the habitat network. In general, the systematic process proposed in this research can be used in planning the sustainable conservation of habitat networks, identifying patterns of ecological security, evaluating and constructing habitat networks in lands with similar climates, and changing and wisely reviewing the conservation area boundaries.

Predictors of the willingness to receive the third dose of COVID-19 vaccine based on the Health Belief Model: A cross-sectional study in South Khorasan province.

Coronavirus disease 2019 (COVID-19) infections have re-emerged in several countries due to reduced levels of antibodies provided by vaccines or the rapidemergenceof viralvariants,such as Alpha, Beta, Gamma, and Delta. A considerable supplementary intervention is recommended, including an additional dose of vaccination. The present study aimed to assess the predictors of the willingness to receive the third dose of the COVID-19 vaccine based on the Health Belief Model (HBM) constructs among people referring to health centers in South Khorasan province. In this cross-sectional-analytical study, 283 people over 18 years old in South Khorasan province were randomly selected using the multi-stage cluster sampling method. The data were collected using a researcher-made questionnaire, including demographic information and health status, knowledge about COVID-19, and the third dose of the vaccine, as well as a questionnaire according to the HBM constructs, which was completed through self-report methods. Finally, the data were analyzed using the Chi-square test and multivariate logistic regression analysis. The mean age of the study participants was 36.57±11.56 years (range of 18-55). The results of multivariate logistic regression analysis demonstrated that gender (P=0.012), marital status (P=0.038), occupation (P=0.013), perceived severity (P=0.005), and cues to action (P=0.018) had a significant direct effect, while perceived barriers (P=0.010) had a significant inverse effect on the willingness to accept the third dose of the vaccine. Moreover, the predictor variables explained about 67.7% of the willingness to receive the third dose of the vaccine. The current study suggested that enhancing the perceived severity of COVID-19, along with healthcare providers' recommendations to receive the vaccine and reducing perceived barriers, can effectively encourage individuals to receive the third dose of the COVID-19 vaccine. These findings can be utilized to develop interventions aimed at promoting the uptake of the booster dose of the COVID-19 vaccine.

Aceria spp. (Acari: Eriophyidae) associated with Amaranthaceae around the world with description of two new species</p >

During field surveys of eriophyoid mites in Razavi and North Khorasan provinces (Iran) in summer 2023, two new Aceria spp. (Acari: Eriophyoidea: Eriophyidae) on Amaranthaceae plants were discovered, illustrated and described: Aceria sodae sp. nov. on Soda rosmarinus (Bunge ex Boiss.) Akhani and Aceria esfarayeniensis sp. nov. on Salsola arbusculiformis Drobow. Furthermore, a list of Aceria spp. on Amaranthaceae species around the world, their type host plants and their relationship to hosts are provided.

Discordance Therapeutic Protocol of Cystic Echinococcosis With WHO Guideline: A Descriptive Study Based on Liver Ultra-Sonographic Data in North Khorasan Province, Northeastern of Iran.

Cystic echinococcosis (CE), a helminth-associated zoonosis caused by Echinococcus granulosus, poses a significant public health problem, particularly in pastoral-rearing regions. The lack of uniform guidelines led to variations in CE management. Based on ultrasound data, the World Health Organization Informal Working Group on Echinococcosis (WHO-IWGE) classification system categorizes cysts into active, transitional, and inactive groups. This study assesses whether the therapeutic approach from liver human operation cases in North Khorasan province aligns with the WHO-IWGE reference based on ultrasound data. The research is based on ultrasound data from liver CE human operation cases collected between 2018 and 2022. This retrospective study investigates the therapeutic protocol for (CE) in North Khorasan Province, Iran, comparing it with the WHO-IWGE guidelines. We collect data from previously registered patients' medical information from our studied area's main CE surgical hospital. Moreover, as the first hospitalized survey in Iran, this study reveals insights into patient demographics, cyst stage prevalence, and treatment modalities. Notably, more than half of the patients were treated for CE1 stage cysts, and CE4 cases, which generally do not require surgery, underwent open surgery. The results suggest a need for adherence to the "watch-and-wait" approach in specific cases. All patients underwent successful surgeries, but we do not have access to follow-up data from patients after discharge. This descriptive study contributes to understanding the implementation of WHO guidelines in a regional context, shedding light on the challenges and variations in CE management. It seems, retraining courses for surgeons are required to update their knowledge of standard CE diagnostic and treatment methods.

Peace Journalism Training for Journalists as a Contribution to PVE in the New Afghanistan

This article presents and discusses results from an exercise in comparative content analysis of news articles about issues of conflict produced by Afghan journalists before and after participating in an internationally sponsored training and mentorship programme in Peace Journalism. The programme was part of a Preventing Violent Extremism (PVE) project, intended to create community resources for resilience, in the information sphere, towards conflict issues contributing to recruitment by non-state armed groups such as Islamic State–Khorasan Province (IS–KP). Peace Journalism is familiar as the basis for media development aid in contexts of conflict; however, its use in an intervention aimed specifically at PVE is relatively new. The results showed that the programme was effective, it is argued, in terms of benefits transferred to and applied by participating journalists. A sample of articles after the training showed a markedly higher Peace Journalism quotient than a baseline sample of articles by the same journalists before it. This suggested that the training and mentorship had successfully stimulated and enabled journalistic agency, taking account of constraints imposed by media structures and wider political and social contexts. The latter have become steadily more onerous under the Islamic Emirate (Taliban) government, in power since August 2021, according to international monitoring organisations. Implications are considered, in light of the findings, for future media development aid to Afghanistan.

Sleep duration and quality are associated with health-related quality of life in adults aged 60years and older.

The current study aimed to determine the association between sleep duration and quality with health-related quality of life (HRQoL) among the Iranian older persons. This study was conducted on a representative sample of 3452 Iranian older people (≥ 60years) who participated in the Iranian Longitudinal Study on Ageing (IRLSA) launched in Neyshabur (Razavi Khorasan province, northeastern Iran). The Persian versions of 12-Item Short Form Survey (SF-12) and Pittsburgh Sleep Quality Index (PSQI) were utilized to measure the level of HRQoL and the level of sleep duration and quality, respectively. Multiple linear regression model was performed to analyze the data and clarify the independent association of sleep duration and quality with HRQoL. More than half of the study population (52.4%) suffered from poor sleep quality. 57.3% and 3.9% of individuals were short sleepers (< 7h) and long sleepers (equal or more than 9h), respectively. The total score of HRQoL was achieved 65.87 ± 20.55. After adjusting the covariates, the results indicated that short sleep duration was negatively associated with HRQoL (P < 0.05), whereas no significant associations were found between long sleep duration and HRQoL (P > 0.05). Interestingly, the results of multiple linear regression model revealed a significant negative association between poor sleep quality and HRQoL (P < 0.001). The current study confirmed that short sleep duration and poor sleep quality were independently associated with lower HRQoL in the older persons. Therefore, interventional courses should be planned to enrich sleep-related healthcare and general HRQoL in the older persons.

Assessing light and quality indicators of fodder at different levels of nitrogen fertilizer based on additive intercropping of barley (Hordeum vulgare) and vetch (Vicilia ervilia) under dryland farming

The intercropping system leads to increasing radiation use efficiency and quality indicators and improving the stability in the production of fodder plants. Intercropping and reducing the use of chemical fertilizers are the most effective methods to achieve sustainable agriculture goals. To investigate light and quality indicators of fodder obtained from barley and vetch additive intercropping at different levels of nitrogen fertilizer, a split plot experiment was performed with a randomized complete block design (RCBD) and 4 replications in two experimental fields in Sisab and Shirvan, North Khorasan Province, Iran, during 2017&amp;ndash;2018. The factor included nitrogen fertilizer at three levels (zero, 50 and 100 Kg.ha&amp;minus;1 (N0, N1 and N2)) and the subfactor included the combination of crops at four levels (barley pure cropping (I1), 15% vetch +100% barley (I2), 30% vetch +100% barley (I3) and 45% vetch + 100% barley (I4) additive intercropping). The results showed the highest fodder dry weight, absorbed radiation, radiation use efficiency, light extinction coefficient, ground cover percentage and leaf area index (LAI) in I3 and N2 in Sisab, and the lowest in I1 and N0 in Shirvan. Also, the highest crude protein (CP) was obtained from I4 and N2 in Shirvan, and the lowest crude protein was obtained from I1 and N0 in Sisab, which was inconsistent with neutral detergent fiber (NDF). The study results showed that increasing the percentage of vetch by 30% in intercropping and using nitrogen fertilizer led to increases in the indicators measured in the experiment.

Estimating the Burden of Severe Cases of COVID-19 in Iran (North Khorasan Province) since the Beginning of the Pandemic: 2019-2021

Background: The death and fear of the COVID-19 pandemic has been a major concern in all countries. Estimating the burden of the disease is one of the indicators that estimates the severity of the damage caused by the disease. This study was performed to estimate the burden of years of life lost due to COVID-19 infection and premature death in North Khorasan.Methods: In this study, all cases of definitive infection (PCR test positive) with COVID-19 from the beginning of the epidemic to April 2021 were included in the study. Disability-Adjusted Life -Years index (DALYs) was used to estimate the burden of the disease.Results: Since the beginning of the epidemic, 63697 definite cases of the disease have been identified (prevalence equal to 7%) and the total number of years of life lost due to disability and premature death was estimated at 24769 years. This number represents 28.7 years per 100,000 populations, which in women and men, this number was equal to 26.6 and 30.8, respectively.Conclusion: According to the results of the study, COVID-19 has caused significant loss of years of life, which due to age and concomitant diseases, deaths are more common in the elderly.

Earliest upper Barremian (Lower Cretaceous) record of bivalve Pinna (Pinnidae) from the Kopet-Dagh Basin, northeastern Iran and its possible predators

The bivalves Pinna sp. cf. cretacea (Schlotheim, 1813) and Pinna cretacea cretacea (Schlotheim, 1813) are systematically described for the first time from the ammonite-constrained upper Barremian (Lower Cretaceous) strata of the Kopet-Dagh Basin, northeastern Iran. This is also, the first record of the Family Pinnidae from the Kopet-Dagh Basin. The specimens were collected from the basal part of the Sarcheshmeh Formation, exposed in the Bojnurd city, North Khorasan province. Additionally, this study includes observations on predation scars (such as scalloped, embayed, and cleft scars), and encrustations by orbitolinids on the Pinna samples. Insights into the contemporary paleoenvironment, characterized by quiet and shallow waters with a soft, muddy substrate, are also discussed.

The Duration and Related Factors in Delayed Diagnosis of Tuberculosis in the southeast of Razavi Khorasan province

The Duration and Related Factors in Delayed Diagnosis of Tuberculosis in the southeast of Razavi Khorasan province

Variability in phenolic compounds, antioxidant capacity and essential oil profile in different tissues of Ferula assa-foetida L. populations in Iran: an opportunity for industrial products

AbstractPlant responses to multiple environmental conditions in the habitat are characterized by intra-species variety. Ferula assa-foetida L. is a native medical plant in Iran, which has a wide range of therapeutic and pharmacological properties. The objective of the current study was to identify changes in phenolic content, antioxidant capacity and essential oil (EO) composition in different tissues (leaf, flower and fruit) of three populations of F. assa-foetida L. (Sarbisheh, Nehbandan, located in South Khorasan province of Iran). The results showed that total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity in the leaves of Sarbisheh were higher than in other treatments. The amount of TPC, TFC and DPPH scavenging activity in leaves was higher than in other tissues in the Sarbisheh population, increasing by 31 and 70%, 41 and 20%, 8 and 13%, respectively, over that of flowers and fruits. The main EO compounds of leaves were α-pinene (11.86–13.56%), β-pinene (6.80–7.99%), bornyl acetate (15.80–17.33%). The main EO constitutes of flowers were Z-propenyl-sec-butyldisulfide (10.12–12.4%), eco-fenchyl acetate (10.87–12.4%), myrcisticin (13.43–15.87%) and α-bisabolol (11.23–13.76%). The primary compounds of fruit EO were β-pinene (9.32–11.26%), (Z)-1-propenyl sec butyldisulf (17.54–18.87%) and (E)-1-propenyl sec butyldisulf (28.32–30.02%). The agglomerative hierarchical clustering showed that three distinguished clusters were identified for population, where Nehbandan and Qaen had the higher similarity and Sarbisheh completely differed from the two ones. Different plant tissues contain varying types of chemicals, with leaves exhibiting higher levels of phenolic content and antioxidant capacity. This characteristic can be beneficial for industries such as food and pharmaceuticals.