Karate Zeon Research Articles

Effect of pesticide treatment of spring wheat plots on seed yields

Introduction. High-quality varietal seeds, which can ensure a gain of 0.2–0.4 t/ha in the yields from their offspring, is one of the most important and cost-effective means to increase the gross grain collection of grain.
 Purpose. To study productive capacities of spring wheat seeds depending on treatment of fields with fungicides and insecticides.
 Materials and methods. The following indicators were evaluated: swelling activity, germination energy, laboratory germinability, initial growth strength, coleoptile length and the number of radicles. Seed samples that after pesticide treatment had showed the best results were tested in field experiments for productive capacities. The experimental plot area was 10 m2, in six replications. Seeds were sown with a seeder SN-10Ts after soybean with a seeding rate of 5 million germinable seeds per hectare. Spring wheat seeds produced in the experimental plots, which were treated with fungicides Akula (0.6 L/ha) and Soligor 425 EC (0.6 L/ha) and insecticides Fas (0.15 L/ha) and Karate Zeon 050 CS (0.15 L/ha) in organogenesis stages VI, VIII and X, were evaluated for productive capacities. The study was carried out on varieties MIP Zlata, Bozhena, MIP Raiduzhna and Diana.
 Results and discussion. Over the study years, the gain in the yields of spring wheat varieties grown from seeds of fungicide-treated plants was 0.23–0.36 t/ha; the gain in the yields of spring wheat varieties grown from seeds of insecticide-treated plants was – 0.24-0.31 t/ha. Pesticide-treated parental plants produced seeds with increased productive capacities: when such seeds were sown, the field germinability increased by 3-5%, and the plant survival – by 5-7%.
 Conclusions. The results indicate that the treatment of vegetating plants of spring wheat varieties in seed plots with fungicides and insecticides is a reliable way to obtain seeds with high productive capacities

Impact of optimized pest control schemes on mandarin yield in the Republic of Abkhazia

Pests of mandarin reduce significantly crop productivity and commercial quality of the crop in Abkhazia. We assessed the impact of optimized mandarin pest control schemes on fruit size and crop yield in the humid subtropics of Abkhazia. The studies were carried out in 2019-2020 on full-aged plantings of cv. Unshiu mandarin in Gulrypsh district of Republic of Abkhazia on the base of Institute of agriculture of Academy of sciences of Abkhazia. The highest yield and quality of fruits was shown by the protection schemes in variants 5 (Confidor extra (0.05 %) + Cytovit (0.15 %) – the 1st treatment Vertimec (0.1%) + Cytovit (0.15 %) – the second treatment; the third and fourth treatments Karate Zeon (0.05 %) + Cytovit (0.15 %)) and 6 (Metomax (0.15 %) + Vertimec (0.1 %) – the 1st treatment; Karate Zeon (0.05 %) + Vertimec (0.1 %) in the other three treatments). The average fruits weight in these variants was up to 72-74 g. This exceeded the fruits weight in the Standard variant by 22.0-25.4 %. The yield was 46.2-44.7 t/ha, which is 36.8-41.3 % higher than the standard variant. Fruits of the 1st grade in the named variants accounted for 63.3-65.6 % of the total yield.

Experience of growing soybeans (Glycine max (L) merryll) on irrigation in the unstable moisture zone of the Stavropol Territory

To obtain a stable harvest of high quality grain, Agrosakhar LLC, located in the Stavropol Territory, used soybean growing technology, which included: the use of modern energy and resource-saving equipment for soil cultivation, sowing and harvesting, cultivation of adapted varieties bred in Russia and Canada - Selecta 302, Vilana, Furio, Kofu, Kyoto, Kanata; introduction of complex fertilizer - azophoska for main soil cultivation, pre-sowing seed treatment with a fungicidal dressing agent Delit Pro and the inoculant Highcoat Super Soy. The system of protective measures included a combination of agrotechnical measures using chemical plant protection products based on monitoring of harmful objects. To combat monocotyledonous and dicotyledonous species of weeds, sowing was treated with Pledge herbicide before germination, followed by a tank mixture of herbicides Bazagran with Harmony in the phase of the first true leaf in soybean plants. The use of the fungicide Akanto Plus together with Karate Zeon and Ampligo Plus ensured effective protection of soybean plants from diseases and pests during the growing season. The technology used for growing soybeans on the farm enables you to consistently get a large and high-quality grain yield. The maximum yield of 2.92 t/ha was obtained by sowing the Kofu variety using the developed cultivation technology. On average, the yield of protein amounted to 0.98, and vegetable fat amounted to 0.59 t/ha. The profitability of soybean grain production on the farm using this cultivation technology is 44.2%.

Technical efficiency of spring rapeseed protection scheme

The species composition of weeds and pests in the crops of spring rape of the variety Nadezhny 92 was determined, the technical effectiveness of the protection scheme of the Syngenta company was evaluated. The results of the study showed that the prevailing species were cereal weeds of 17.9 units / m2. Dicotyledonous weeds amounted to 13.6 units / m2. A decrease in the moisture supply of the vegetation period entailed an increase in weediness. The highest weediness was recorded in 2019, when the number of weeds was higher than in 2017 and in 2018 on 23.5 - 18.0 pcs / m2. Of the pests, cruciferous fleas, rapeseed beetle, rape sawfly, and caterpillars of cabbage were found in the crop. Spring rape protection scheme: presowing treatment with Cruiser Rapeseed, KS, Zellek Super, KE Galera Super, BP, Karate Zeon, MKS showed high technical efficiency. Technical efficiency against cereal weeds was 84.4%, against dicotyledons - 90.4%. On average, the protection effect on spring rape crops was 86.3%. The use of an insecticide allowed reducing the number of pests in crops below the economic threshold of harmfulness or completely destroying them.

Treating honey bees with an extremely low frequency electromagnetic field and pesticides: Impact on the rate of disappearance of azoxystrobin and λ-cyhalothrin and the structure of some functional groups of the probabilistic molecules

The purpose of these laboratory tests was to assess the impact of 50 Hz EMF (electromagnetic field) on the disappearance of azoxystrobin (active ingredient (AI) of Amistar 250 SC) and λ-cyhalothrin (AI of Karate Zeon 050 CS) in the body of honey bees (Apis mellifera) and the structure of some functional groups of the probabilistic molecules in their organisms. Amistar 250 SC (an azoxystrobin-based fungicide; ABF) and Karate Zeon 050 CS (a λ-cyhalothrin-based insecticide; CBI) are plant protection products (PPPs) applied to bee-pollinated-crops. Chromatographic methods were used to assess the rate of AI disappearance. EMF affected the rate of disappearance of azoxystrobin and λ-cyhalothrin in bees within 6 h of intoxication. When these substances were used separately their disappearance in the presence of EMF slowed from 12.6% to 10.5% h−1 and from 9.2% to 4.8% h−1, respectively, and accelerated when used in a mixture, from 14.1% to 14.7% h−1 and from 9.3% to 11.5% h−1 respectively. Fourier Transform Infrared (FTIR) spectroscopy was used to analyze changes in the functional groups of the probabilistic molecules of the tested bees. To obtain the information about the spectra variations we used the Principal Component Analysis. It has been shown, that EMF statistically significantly interferes with amide I and II, symmetric PO32− group from DNA, RNA and phospholipids vibrations. It also increased the number of changes of functional groups of the probabilistic molecules caused by ABF, but at the same time limited the changes in the functional groups studied in bees treated with CBI and a mixture containing both of them. In addition, exposure to EMF in bees treated with fungicide or insecticide, separately, and with both preparations caused differences (p < 0.05) in the secondary structure of proteins compared to controls. The obtained results indicate that EMF may affect the rate of metabolism and the detoxification process of pesticides in bees, depending on the AI of PPPs, applied individually or together. However, further detailed research is required to explain the mechanism of EMF as a detoxification modulator.

OPTIMIZATION OF PESTICIDES RESIDUES ANALYTICAL CONTROL IN CEREAL CROPS PROTECTION SYSTEM

Relevance. The scientific substantiation of the method selection, the development of conditions for qualitative identification and quantitative determination of Axial 050 EC, Peak 75 WG, Tilt 250 EC, Magnello 350 EC, Enzhio 247 SS, Karate Zeon 050 CC pesticides (by its simultaneous presence) used in cereal crops protection system was given in the article, as well as preparation conditions for the analysis of water and air samples were described, which will allow to control the established hygienic standards and minimize pesticide negative impact on population health and the environment.&#x0D; Objective of the research is to develop methods for the analytical determination of 9 active substances of 7 pesticide preparations applied in the wheat and barley protection system with its simultaneous presence in water and air.&#x0D; Materials and methods. Chromatographic analysis was performed by Shimazu (Japan) liquid chromatograph. Chromatograph control, recording, analysis and storage of chromatographic data were performed using S/w LC solution software. The package of IBM SPSS StatisticsBase v.22 and MS Exel statistical programs was used for statistical processing of results.&#x0D; Results. The developed optimal conditions of sample preparation and chromatographic determination in the combined presence of 9 different chemical classes pesticides used in the cereal crops protection system will allow to control their content with the limit of quantitative determination of each compound 0,001 mg/dm3 in the water sample, and 0,05 mg/m3 in the air, thus allowing control the established hygienic standards of these compounds in the water and in the working zone air.&#x0D; Conclusions. The conditions for simultaneous determination of prosulfuron and pinoxaden herbicides, thiamethoxam and lambda-cyhalothrin insecticides, fludioxonil, sedaxane, tebuconazole, propiconazole, diphenoconazole fungicides in its combined presence in the water or air samples by high-efficiency liquid chromatography method were elaborated allowing the efficiency of analysis to be increased and its total price to be reduced.

Thrips are dangerous pests of vegetable crops

Goal. To provide information about dangerous pests of vegetable crops — thrips. To describe the morphology and biology of the pest, the methods of spreading and harmfulness, provide information on the necessary phytosanitary measures to restrict its development and reduce the harmfulness, and to justify the need to use alternative biological methods to control the population of thrips in the conditions of the Southern steppe of Ukraine on an example of the most common of them — tobacco thrips (Thrips tabaci Lind.).&#x0D; Methods. Informational and analytical. The collection of information was carried out in accessible specialized literature and the Internet.&#x0D; Results. The information about sucking pests of vegetable crops - thrips is given. Thrips are known for a long time, they belong to wide polyphages, that is they harm a wide range of cultural and wild plants (from 100 to 400 spesies). On an example of the most common of them — tobacco thrips (Thrips tabaci Lind.) there is given information about the morphology and biology of the pest, the methods of its spreading and harmfulness, as well as the information on the necessary phytosanitary measures for restriction its development and reduction of the harm. This most widespread and dangerous polyphage, due to its close connection with plants, where its entire life cycle develops - from eggs to imago, has inhabited almost all greenhouses. In the natural conditions of the southern region of Ukraine, the tobacco thrips were found on large areas of vegetable crops with the seedling method of their cultivation, mainly cucumbers, tomatoes, onions, cabbage, watermelons, and the like. It harms bulbs in vegetable stores, which leads to a deterioration in the quality of garden plants and gardening material. The reaction of the plant to the damage by the trips is desensitization, which is manifested in growth retardation, distortion and loss of turgor of damaged leaves, which later become yellow and dries. Imago winter, mostly females, in greenhouses - in different slits, and in open ground - in the upper layer of soil at a depth of 7-10 cm, under plant remains or in the rootstock of perennials or weeds. At temperatures below 0 ° С and in the absence of snow cover, insects die. The pest spreads at all stages of development - eggs, larvae, imago - with planting material of vegetable crops (with plants or in soil), transport, containers, workers' tools, their clothes or footwear. This also occurs when collecting, packing and selling contaminated products. This is especially true for cabbage, with which the thrips can get to the greenhouse or indoor plants. In the conditions of the steppe and forest-steppe zones of Ukraine, the tobacco thrips can develop in open soil at temperatures over 10 ° C during the growing season from the second decade of May to the end of September, causing significant damage to vegetable crops. If this is the case, the pest can give 3-4 to 5-6 generations, depending on the weather conditions. In the population control of tobacco thrips, satisfactory results are received by systematic cultivation of plants with the following insecticides: Akketlik, Aktar, Phyitoverm, Karate Zeon, Confidor Maxi (with irrigation water) and others. It is obligatory to change preparations during the growing season. On the onion fields thrips is successfully reduced with insecticide of natural origin Spintor when using periodic spraying of plants. Because thrips are very resistant pests, therefore, in addition to insecticides, the use of predatory mites Amblyseius cucumeris, A. barken, A. derenerans, as well as predators of Orius laevigatus, O. magusaculus can be used to destroy them. Taking into account that nowadays the protection of vegetable crops takes the direction for the use of biological agents against pests - polyphages, special attention should be paid to the use of natural enemies of the tobacco thrips.&#x0D; Conclusions. In the natural conditions of the southern region of Ukraine, tobacco thrips were found on large areas of vegetable crops, therefore, there are all reasons for phytosanitary surveys of open-air vegetable crops to detect and eliminate this dangerous pest. Taking into account that nowadays the protection of vegetable crops takes the direction for the use of biological agents against pests - polyphages, special attention should be paid to the use of natural enemies of the tobacco thrips.

Lambda-cyhalothrin affects swimming activity and physiological responses of Daphnia magna

λ-cyhalotrin is a pyrethroid pesticide used for protection of crops against various insect pests. Knowledge on behavioural and physiological responses of non-target organisms such as cladocerans is very limited. Daphnia is a sensitive organism commonly used in determination of ecotoxicological risk for various substances introduced to aquatic environment, however the main experimental endpoints used such as mortality or immobilisation may not be sufficient to evaluate subtle alterations in zooplankton. The aim of the present study was to evaluate swimming behaviour and physiological parameters of Daphnia magna exposed to λ-cyhalothrin (Karate Zeon 050 CS) at concentrations of 0.05, 0.5, 5 and 50 μg L−1 for 2, 24 and 48 h. The results showed that λ-cyhalothrin affected D. magna swimming behaviour inducing a concentration-dependent inhibition of swimming track density, speed and turning ability. Depression of physiological parameters such as heart rate and thoracic limb activity was also noted. The results suggest that in natural conditions swimming behaviour and physiological endpoints of D. magna may be disturbed by environmental concentrations of λ-cyhalothrin leading to ecological consequences.

Removal of some commercial pesticides from aqueous dispersions using as flocculant a thymine-containing chitosan derivative

A novel thymine-containing chitosan derivative has been designed, synthesized, and used for the first time in this work for removal of some commercial pesticides from synthetic wastewater by coagulation/flocculation. The chitosan-thymine conjugate was characterized by 1H NMR and FT-IR spectroscopy, and its flocculation performance was evaluated by UV–Vis spectroscopy in comparison to non-modified chitosan. The commercial pesticides used in this investigation were Fastac 10 EC and Karate Zeon (with alpha-Cypermethrin and lambda- Cyhalothrin, respectively, as active pyrethroid ingredients), Novadim Progress (Dimethoate as active organophosphoric ingredient), and Bordeaux mixture. The experimental results demonstrate good removal efficiency for Karate Zeon (between 80 and 85%), a maximum of around 90% for Fastac 10EC and Novadim Progress, and more than 95% for the Bordeaux mixture, irrespective of the polycation structure. According to the zeta potential values recorded in the optimum dose interval, the flocculation of pyrethroid pesticides particles took place mainly by means of the charge neutralization mechanism (close to zero), whereas the flocculation of Novadim Progress and Bordeaux mixture particles has been achieved by the synergistic effect of charge neutralization and hydrogen bonding (Novadim Progress) or copper ion binding by the amine and thymine groups (Bordeaux Mixture) (negative values).

The pyrethroid λ-cyhalothrin induces biochemical, genotoxic, and physiological alterations in the teleost Prochilodus lineatus

The λ-cyhalothrin (CL) is a globally used pyrethroid insecticide that has been detected in different water bodies worldwide. However, studies on the effects of CL on freshwater fishes are still incipient. In this context, we evaluated the acute effects of a commercial formulation containing CL (Karate Zeon® CS 50) in juveniles of the teleost Prochilodus lineatus exposed for 96 h to four concentrations of the active ingredient (5, 50, 250 and 500 ng.L−1). Biochemical, physiological, and genotoxic biomarkers were evaluated in different organs of the fish. Exposure to CL induced significant changes in the enzymatic profiles of P. lineatus, with specific alterations in biotransformation enzymes and antioxidant defence in different tissues. Lipid peroxidation was observed in fish gills and kidney. Increases in esterases were observed in the liver of fish exposed to all CL concentrations evaluated, whereas acetylcholinesterase activity decreased in the muscles of fish at all concentrations. CL also promoted osmoregulatory disorders, with decreases in calcium and magnesium gill ATPases, with consequent hypocalcaemia, in addition an increase in sodium-potassium ATPase activity was observed in the gills of fish exposed to the highest CL concentration, probably in order to compensate a reduction in plasma sodium. Besides, increases in DNA damage were observed in the erythrocytes of fish exposed to all CL concentrations. Thus, despite the low CL concentrations and the short exposure time, this pyrethroid caused hematological adjustments, oxidative stress, osmoregulatory disorders, and DNA damage in P. lineatus, showing that the species is highly sensitive to the deleterious effects of CL.

Effects of insecticidal seed treatments and foliar sprays in winter oilseed rape in autumn on insect pests and TuYV infection

In this study, the effect of the four different insecticides Karate Zeon (a.i. lambda-cyhalothrin), Elado (clothianidin + beta-cyfluthrin), Fortenza Force (cyantraniliprole + tefluthrin) (only 2015), and Lumiposa (cyantraniliprole) (only 2016 and 2017) on the insect autumn pests Delia radicum, Psylliodes chrysocephala, and aphids (mainly Myzus persicae) and Turnip Yellows Virus in winter oilseed rape was investigated in 3 years of field trials. The results showed that the effects of the Elado seed treatment on P. chrysocephala depend on the time of migration of adults. Due to delayed immigration of beetles into the field in 2015/2016 and 2017/2018, there was no clear effect of the seed treatment on the number of larvae per plant. In contrast in 2016/2017, the beetles colonized the crop in early September, and the number of larvae was significantly reduced by Elado. Cyantraniliprole and clothianidin seed treatments seem to support crop emergence independent of insect pests assessed. Spraying of the pyrethroid Karate Zeon in autumn significantly decreased the number of larvae in all years. Also the number of next-generation beetles was significantly reduced only by Karate Zeon. In all years, the aphid infestation was significantly reduced only by Elado seed treatment. The infection rate by TuYV was very high in all years and only slightly reduced by Elado seed treatment. There was only minimal occurrence of Delia radicum in all years. Cyantraniliprole products did not show any influence on the assessed insect pests in all years. There was no significant effect of any treatment on yield, but in 2015/2016 and 2016/2017, the highest yield was obtained in the Elado seed treatment.

Effects of thiacloprid, tau-fluvalinate and lambda-cyhalothrin on overwintered pollen beetles (Brassicogethes aeneus (Fabricius)) and their offspring in oilseed rape

To prevent damage by pollen beetles (Brassicogethes aeneus (Fabricius), syn. Meligethes aeneus (Fabricius)), frequent insecticide applications are often necessary. It would be an advantage if treatments for pollen beetle control would not only avoid yield losses by bud damage by overwintered pollen beetles, but also minimize pest reproduction, and also have side effects on the population development of other pests present in the crop. The effects of the neonicotinoid Biscaya (a.i. thiacloprid) and the pyrethroids Mavrik (tau-fluvalinate) and Karate Zeon (lambda-cyhalothrin) applied at different growth stages of winter oilseed rape on the abundance of overwintered pollen beetles were determined in field trials in Germany (2013–2015). In addition, effects on the two larval instars and new generation of adult pollen beetle were studied. Biscaya and Mavrik significantly reduced the number of overwintered pollen beetles up to seven days after application, whereas Karate Zeon had no effect. Application of Biscaya at the beginning of flowering resulted in a high mortality of L1-larvae in all years. The number of premature L1-larvae dropping down from the plants during the first week after application increased up to 425% compared to the control. The number of L2-larvae dropping down to the ground for pupation was significantly reduced by insecticide applications at different growth stages except for Karate Zeon. In Karate Zeon-treated plots, the number of L2-larvae dropping to the ground increased up to 42% compared to the control. In accordance with the reduced number of L2-larvae in Biscaya- and Mavrik-treated plots, fewer new-generation pollen beetles emerged in the field trials near Braunschweig, efficacy varying between 57 and 76% in Biscaya-treated plots and 32 and 57% in Mavrik-treated plots in 2014 and 2015, respectively. The results indicate that Mavrik, and especially Biscaya, are effective in controlling pollen beetles, reducing infestation pressure and thereby insecticide treatment frequency in following years.

Impact of insecticides on oilseed rape bud infestation with eggs and larvae of pollen beetle (Brassicogethes aeneus (Fabricius))

One of the main insect pests in oilseed rape is the pollen beetle (Brassicogethes aeneus (Fabricius), syn. Meligethes aeneus). To maximize efficiency of control of this pest, insecticides are required that ideally, not just prevent yield losses by bud feeding of overwintered pollen beetles, but simultaneously minimize the reproduction of the pest, thereby reducing the size of the following generation infesting next year’s oilseed rape. The neonicotinoid active substance thiacloprid is known to reduce bud infestation with eggs and larvae. However, the mechanisms by which this occurs as well as the effects of other active substances are not known. In this study, the effects of the neonicotinoid insecticide Biscaya (a.i. thiacloprid) and the pyrethroids Mavrik (tau-fluvalinate) and Karate Zeon (lambda-cyhalothrin) applied at the bud stage of winter oilseed rape with recommended field rates on infestation of buds with eggs and larvae of pollen beetles were tested in field trials in Germany in 2013–2015. In additional greenhouse experiments, it was investigated whether effects on bud infestation were caused by lethal effects on pollen beetle or by insecticidal residues on plants causing sublethal effects. In the field trials, application of Biscaya and Mavrik significantly reduced the percentage of buds containing eggs and larvae in contrast to Karate Zeon. In 2014 and 2015, 14 days after application, bud infestation on the main raceme was reduced by 86 and 82%, respectively, in Biscaya-treated plots and by 51 and 71%, respectively, in Mavrik-treated plots compared to the untreated plots. In the greenhouse experiments, the lowest percentage of bud infestation with eggs and larvae was recorded on Biscaya-treated plants whereas on Mavrik-treated plants, there was no significant difference compared with the control. The results of the field trials show that Biscaya and Mavrik reduced oilseed rape bud infestation with eggs and larvae of pollen beetles primarily by lethal effects on overwintered pollen beetles or by repellency. However, Biscaya had additional effects on egg laying, which was supported by the greenhouse experiments.

Removal of the commercial pesticides Novadim Progress, Bordeaux mixture and Karate Zeon by pullulan derivatives based flocculants

Cationic pullulan derivatives have been synthesized and evaluated, for the first time, as flocculants for the separation of the commercial pesticides, Novadim Progress (organophosphoric type), Bordeax mixture and Karate Zeon (pyrethroid type) from synthetic wastewater. The investigated polymer samples contained either pendent tertiary amine or quaternary ammonium salts groups. The separation efficiency was followed by UV–Vis spectroscopy, while the information regarding the mechanism involved in the separation of pesticide particles have been obtained by zeta potential. UV–Vis spectroscopy data showed strong pesticide particles/polycation interactions in case of Novadim Progress and Bordeaux mixture (maximum pesticide removal between 90% and 98%). Good separation efficiency (around 80%) of Karate Zeon emulsion was also noticed. The zeta potential measurements indicated that the charge neutralization was the common flocculation mechanism for the removal of these pesticides. In addition, the hydrogen bondings and chelation of copper ions by amide and/or tertiary amino groups of the polycations had a noteworthy contribution to the pesticide removal.

Effects of insecticide application on parasitism rates of pollen beetle larvae (Brassicogethes aeneus (Fabricius)) by tersilochine parasitoids

Larval parasitoids can substantially reduce the population density of the pollen beetle [Brassicogethes aeneus (Fabricius), syn. Meligethes aeneus (Fabricius)]. The most abundant tersilochine parasitoids of pollen beetle are Tersilochus heterocerus, Phradis interstitialis and P. morionellus. The main activity of these parasitoids was observed in the period shortly before flowering to full flowering of oilseed rape. Insecticide applications during this period may have negative effects on parasitoids. In the present study, the effects of the insecticides Biscaya (a.i. thiacloprid), Mavrik (a.i. tau-fluvalinate) and Karate Zeon (a.i. lambda-cyhalothrin) applied during the bud or flowering stage of winter oilseed rape on parasitization of pollen beetle larvae by T. heterocerus were studied in 12 field trials at different locations in Germany in 2013–2015. The effects on parasitism by Phradis spp. were assessed in 2015. Parasitism of pollen beetle larvae by T. heterocerus was found in all field trials in all experimental years, but in most trials not before full flowering. Maximum percentage of parasitized larvae at different locations ranged between 3.4 and 16.8% in 2013, 8.3 and 22.4% in 2014 and from 11.1 to 29.1% in 2015. Levels of parasitism were not significantly different between the untreated control and insecticide treatments within each location. In contrast to T. heterocerus, Phradis spp. was not detected at all locations and not before flowering declining. In field trials at Lucklum and Puch, the maximum level of parasitism by Phradis spp. was 9.4 and 18.3%, respectively. No significant effect of insecticide application on parasitism by Phradis spp. was observed between the treatments. The results of this study showed that the insecticides used in the field trials did not affect parasitization of pollen beetle larvae by T. heterocerus and Phradis spp., regardless whether applied at the bud stage, at the beginning of flowering or full flowering.

Effects of insecticides used against the European corn borer on thrips abundance on maize

The effect of a single chemical treatment against Ostrinia nubilalis (Hbn.) on thrips abundance on maize in south-eastern Poland was evaluated. Two insecticides: Karate Zeon 050 CS, containing lambda-cyhalothrin, and Proteus 110 OD, containing thiacloprid with deltamethrin, were tested. Maize was sprayed in the second ten days of July, during the abundant occurrence of O. nubilalis larvae, which coincided with the population peak of thrips on plants. The tested active substances showed high effectiveness against thrips, but a better effect, reflected in a decrease in thrips abundance, was found for the mixture of thiacloprid with deltamethrin. The tested insecticides significantly reduced the population of thrips for up to 14 days after treatment.

Efficiency of joint application of microfertilizers and plant protection products on bread winter wheat crops

Aim. To determine yielding capacity and economic efficiency of joint application of microfertilizers and plant protection products on winter wheat crops in the Right-bank Forest-Steppe of Ukraine.Materials and methods. The researches (2012-2014) were conducted on the fields of the V.M. Remeslo Myronivka Institute of Wheat of the National Academy of Agrarian Sciences of Ukraine on commodity crops of bread winter wheat with varieties Kolos Myronivshchyny, Myronivska storichna, Natalka and Yuviliar Myronivsky treated with microfertilizers together with plant protection products in the IV, VIII and X stages of organogenesis. The treatment was carried out with microfertilizers Tseovit Micro Grain and Tseovit the Ovary Plus (1 l/ha) together with fungicide Falcon 460 EC (0.5 l/ha) and insecticide Karate Zeon 050 CS (0.2 l/ha). The accounting area of plots treated with fertilizers was 10 m2 in four replications. Agrotechnics in the experiments was common in the Right-bank Forest-Steppe of Ukraine. The crop was harvested by “Sampo-130” combine and recalculated to standard (14%) grain moisture.Results. Resulted from the studies conducted in 2012-2014 it was found that at joint application of microfertilizers and plant protection products during spring-summer vegetation of bread winter wheat the highest yield was obtained when processing crops in the VIII stage of organogenesis. During the years of research, yielding capacity of the varieties in average increased by 1.07–1.15 t/ha as compared with the control.Calculations show that the level of profit for the varieties during years of the research increased in average by 1257-1958 UAH/ha in comparison with the control. The highest profit level (9736–10041 UAH/ha) was obtained from the application of microfertilizers Tseovit the Ovary Plus (1.0 l/ha) and Tseovit Micro Grain (1.0 l/ha) together with plant protection products in the VIII stage of organogenesis, thus exceeding the control by 1653–1958 UAH/ha.When using microfertilizers on elite winter wheat crops, the profit would increase by 3500-4700 UAH/ha in comparison with the control.Conclusions. The use of microfertilizers together with plant protection products on winter wheat crops during the spring and summer vegetation makes it possible to increase yielding capacity of 1.07 to 1.15 t/ha and to increase the net profit from the additional grain by 20–24%.

Effective preparations of protection of winter wheat against main pests in conditions of the Precaucasian steppe zone

In 2009–2014 in conditions of the Precaucasian steppe zone, biological effectiveness of the following preparations was achieved in the fight with main pests of the winter wheat: Actara, B-58 New, Decis Profi, Diazinon Euro, Karate Zeon, Konfidor Extra, Cruiser, Kinmiks, Mospilan, Picus, Sirocco, Scenic Combi, Tabu, Tiara, Fastac, Shaman, Eforie, and active substances of the preparation thiamethoxam + difenoconazole + fludioxonil. Their application by spraying vegetating plants and the presowing treatment of seeds allows the successful fight with the black wheat fly, the cereal ground beetle, the sunn pest, with the molding of seeds, the common bunt, and root rots of the Fusarium–Helminthosporium etiology.

Ефективність сумісного застосування біодобрив і засобів захисту рослин у технологіях вирощування пшениці м’якої озимої

Ефективність сумісного застосування біодобрив і засобів захисту рослин у технологіях вирощування пшениці м’якої озимої

THE EFFECT OF SELECTED PESTICIDES ON THE GROWTH OF ENTOMOPATHOGENIC FUNGI HIRSUTELLA NODULOSA AND BEAUVERIA BASSIANA

The effect of three pesticides used in corn protection: Targa Super 05 (chizalofop-Pethyl), Roundup 360SL (glyphosate) and Karate Zeon 050CS (lambda-cyhalothrin), on the growth of entomopathogenic fungi Hirsutella nodulosa Petch and Beauveria bassiana (Bals.) Vuill. was evaluated under laboratory conditions. Fungi isolates were cultured on Sabouraud’s medium with addition of pesticides at three different concentrations. H. nodulosa was more susceptible to pesticides than B. bassiana. The most inhibitory effect on tested entomopathogenic fungi showed chizalofop-P-ethyl herbicide. Lambda-cyhalothrin at the recommended field dose showed minor toxic effect on entomopathogenic fungi, which suggests the possibility of compatible use of this insecticide with biopesticides based on the tested species.