Establishment and application of efficient protoplast isolation and transformation system from leaves of multi-genotype poplars.

Formulae for predicting the peak dynamic displacement of isolation systems with single friction pendulum bearings

Experimental and numerical study of a novel slope-type sliding isolation system for seismic protection of electrical equipment

Modeling and experimental validation of a novel hydraulic inertia-type vertical isolation system

A fuzzy-controlled semiactive electromagnetic seismic isolation system for near-fault and far-field motions

This paper investigates the application of negative stiffness systems for vibration isolation in civil engineering applications, particularly focusing on pipelines, buildings, and bridges subjected to dynamic loads such as earthquakes, traffic vibrations, and wind forces. These systems, which function by counteracting external forces, have the potential to enhance the structural resilience of infrastructure. The research integrates both mathematical modeling and experimental testing to assess the vibration isolation characteristics of negative stiffness systems. The methodology follows the approach introduced by Adar et al. (2022) in his study of negative stiffness systems for vibration isolation in pipelines. Finite element analysis (FEA) is used to model the system and predict its behavior under dynamic loads, while experimental setups validate the theoretical predictions. By comparing the results from both approaches, the study demonstrates the effectiveness of negative stiffness systems in isolating vibrations across different frequency ranges, particularly where traditional vibration isolation techniques, such as mass-spring dampers or viscoelastic materials, fall short. The findings indicate that negative stiffness systems can achieve substantial vibration reduction, making them a highly viable solution for enhancing structural performance in dynamic environments. These results contribute to advancing the use of negative stiffness technologies in civil engineering, paving the way for more efficient vibration isolation systems in infrastructure that faces extreme dynamic loading conditions. The study provides insights for future research in the integration of negative stiffness in resilient infrastructure design.

This paper introduces a novel seismic base isolator termed the "pendulum column with vertical negative stiffness (PC-VNS)," which functions similarly to a pile foundation while incorporating quasi zero stiffness (QZS) characteristics to modify the structural foundation connection in weak soil and effectively extend the natural period. The system's nonlinear behavior enables it to reduce both displacement and acceleration, even when employing conventional damping coefficients of 5%. The theoretical framework was validated using MATLAB analyses of multiple earthquake records and finite element modeling in ABAQUS. Results from earthquake scenarios demonstrate the system's ability to avoid resonance phenomena while maintaining controlled responses with standard damping. Compared to a conventional fixed-base frame with a 0.4-second natural period, the PC-VNS system achieves a significant reduction in input energy in most cases. The performance of the system is strongly influenced by the maximum allowable range of motion, with improved results observed for smaller displacement limits. The pile-like behavior of the PC-VNS improves soil-structure interaction, contributing to both seismic isolation and foundation reinforcement, thereby offering a promising solution for earthquake-resistant design.

Marine vessels undergo wave-induced motions that amplify impact loads during ship-based aircraft landings, challenging the structural integrity and service safety of aero-engine isolation systems. To address this challenge, this study establishes a coupled ship-fuselage-engine model, investigating the damping performance of a turboprop engine isolation system under impact loads induced by ship heave. The model establishes an equivalent representation of the dynamic interaction between wave-induced ship heave motion and landing impact. It is experimentally validated using a high-energy drop-impact test platform, demonstrating high predictive accuracy with maximum deviations below 5% in vibration amplitude and under 8% in pulse width. As the ship heave velocity increases from 0 to 0.793 m/s, the peak vibration amplitudes rise significantly from 52.48 to 86.72 m/s 2 at the front-left position, from 55.17 to 93.88 m/s 2 at the front-upper position, and from 64.54 to 107.05 m/s 2 at the rear-left position. Further analysis reveals that the damping efficiency of the front isolators improves notably with increasing impact energy, attributed to the nonlinear hysteretic behavior of the isolation rubber. These findings provide valuable insights for optimizing aero-engine isolation systems in shipboard landing applications.

ABSTRACT Over recent decades, the collection of seismic data has improved the understanding of near‐fault ground motion effects, which involve both horizontal and vertical components. Among the most significant effects are fault‐normal directivity, which concentrates seismic energy into an intense, long‐period pulse, and fault‐parallel fling step, which causes permanent ground displacement. In dip‐slip faulting scenarios (such as reverse and normal faults), significant vertical acceleration also occurs, as highlighted by recent studies, which have shown that vertical acceleration can exceed the horizontal component at short spectral periods. This research proposes a systematic approach to evaluate the combined effects of the vertical and horizontal components of near‐fault ground motion – including pulse‐like effects – for different near‐source scenarios. The approach is applied to base‐isolated structures equipped with high‐damping rubber bearings (HDRBs), either alone or in combination with flat slider bearings (FSBs). The results, consistent with previous experimental and numerical studies on similar isolation systems, indicate that the vertical component does not influence the horizontal response of the hybrid isolation system or the superstructure, but it can cause uplift of FSBs, cavitation of HDRBs, and very large vertical accelerations in the superstructure. Furthermore, for scenarios similar to the one considered, they provide insight into the fault distances at which these phenomena may pose significant challenges for base‐isolated buildings.

Islamic education policy plays a strategic role in shaping religious identity, governance, and social cohesion in Muslim-majority countries. Despite sharing Islam as a foundational reference, Indonesia and Saudi Arabia have developed distinct policy orientations shaped by divergent philosophical commitments, legal frameworks, and sociocultural contexts. Existing studies have primarily examined these systems in isolation or through a single analytical lens, leaving a limited comparative understanding of how multiple dimensions interact to shape Islamic education policy. This study addresses this gap by comparing Islamic education policies in Indonesia and Saudi Arabia through philosophical, juridical, and sociocultural perspectives. The study employed a qualitative-comparative approach based on library research. Data were collected from primary sources, including national education laws and official policy documents, as well as secondary sources such as peer-reviewed journal articles and academic books. Data were analyzed using qualitative content analysis to identify patterns of convergence and divergence across the three analytical dimensions. The findings reveal that Indonesia adopts an integrative and pluralistic model of Islamic education, combining Islamic values with a secular-constitutional framework that emphasizes religious moderation and social diversity. In contrast, Saudi Arabia implements a centralized and theologically uniform model grounded in Sharia-based governance and Salafi doctrinal orientation. Socioculturally, Indonesia’s multicultural context encourages adaptability, while Saudi Arabia’s relative homogeneity supports policy uniformity, albeit with gradual reforms under Vision 2030. This study contributes theoretically by proposing an integrative analytical framework that explains how philosophy, law, and sociocultural context jointly shape Islamic education policy. Practically, it offers insights for developing Islamic education systems that balance theological integrity with inclusivity and global educational demands.

Extracellular vesicles (EVs) secreted by Fusarium oxysporum play an important role in the process of its infestation of the host, but the in vitro research system for EVs of F. oxysporum (Fo-EVs) has not yet been improved, and the mechanism of its action remains unclear. In this study, particle size distribution, particle concentration, number of particles per unit of protein, number of particles per unit of mycelial biomass, and concentration of contaminated proteins were used as indicators to evaluate the yield and purity of Fo-EVs. The optimal method for Fo-EV preparation and extraction was screened by comparing liquid culture, solid culture, and solid culture with enzymatic cell wall hydrolysis. The optimal system for Fo-EVs separation and purification was screened by a pairwise combination of three primary methods (Ultracentrifugation (UC), Ultrafiltration (UF), and Polyethylene glycol precipitation method (PEG)) and two secondary methods (Size-exclusion chromatography (SEC) and Aqueous two-phase system (ATPS)), respectively. The protein composition was identified via mass spectrometry technology, followed by GO annotation and GO enrichment analysis using whole-genome proteins as the background. Based on these steps, a Fo-EV protein library was constructed to reveal Fo-EV’s most active biological functions. The results showed that solid culture combined with the UC-SEC method could effectively enrich Fo-EVs with a typical cup-shaped membrane structure. The obtained Fo-EVs had an average particle size of 253.50 nm, a main peak value of 200.60 nm, a particle concentration of 2.04 × 1010 particles/mL, and a particle number per unit protein of 1.09 × 108 particles/μg, which were significantly superior to those of other combined methods. Through proteomic analysis, 1931 proteins enriched in Fo-EVs were identified, among which 350 contained signal peptides and 375 had transmembrane domains. GO enrichment analysis revealed that these proteins were mainly involved in cell wall synthesis, vesicle transport, and pathogenicity-related metabolic pathways. Additionally, 9 potential fungal EV markers, including Hsp70, Rho GTPase family, and SNARE proteins, were screened. This study constructed an isolation system and a marker database for Fo-EVs, providing a methodological and theoretical basis for in-depth analysis of the biological functions of Fo-EVs.

Pulsed Field Ablation Using a Novel Biphasic Catheter vs Thermal Ablation for Paroxysmal Atrial Fibrillation: InsightPFA Trial.

Protoplast isolation and transient expression in the precious and economically important tree Toona ciliata.

Investigations on vertical and horizontal behavior of a three-dimensional isolation system for seismic isolation and subway-induced vibration control

Exploring the regulation mechanism of digestibility of starch-polyphenol-protein ternary system by pH from multiple perspectives.

Development of a three-directional isolation system for LNG storage tanks subjected to near-fault earthquakes

Abstract This study presents a novel integration of impact hammer-based experimental modal analysis with Random Forest Regression (RFR) to rapidly characterise the frequency-domain dynamic behaviour of Carbonyl Iron Particle (CIP)-based Magnetorheological Elastomers (MREs) under varying magnetic fields. Using only applied current and excitation frequency as input features, the RFR model predicts FRF amplitude, phase, and coherence with R 2 values exceeding 0.96 across both low-frequency (0–70 Hz) and high-frequency (> 70 Hz) regimes. This hybrid experimental–computational framework significantly reduces the number of repeated tests required, enabling faster parametric studies and paving the way for real-time, AI-enhanced tuning of smart vibration isolation systems.

Dynamic response analysis of the composite cylindrical cabin with vibration isolation system in supersonic airflow

Design and analysis of a quasi-zero stiffness vibration isolation system with load adaptability

Under complex working conditions, multidirectional vibration and impact seriously restrict the stable operation and service life of engineering structures and precision equipment. Traditional cushioning and vibration isolation systems focus on single‐direction nonzero stiffness design, which is difficult to meet the needs of multidimensional impact and vibration coupling conditions. For this reason, this work draws inspiration from efficient energy‐absorbing structures in nature and proposes an integrated bionic quasi‐zero‐stiffness (QZS) structure design strategy that incorporates cuttlefish bone‐mimicking S‐shaped structure with a beetle shell‐mimicking center ring cross structure. It is shown that the S‐shaped cubic cross center ring‐cross structure (S‐CCCRCS) developed through this design strategy exhibits a distinct QZS plateau and achieves outstanding energy absorption performance in the X , Y , and Z directions. Under impact loading, the S‐CCCRCS structure shows excellent cushioning performance with the lowest peak impact loads in all three directions; at the same time, due to its three‐dimensional QZS, the structure achieves highly efficient vibration isolation in the multidirectional low‐frequency range. The bionic composite QZS structure proposed in this work provides a feasible solution for high‐performance, multidirectional cushioning and vibration isolation in the fields such as rehabilitation medicine and smart devices.