Isolation Of Polysaccharides Research Articles

Isolation of a novel polysaccharide from Tribonema minus and preparation of polysaccharide–selenium nanoparticles with enhanced antioxidant activity

Isolation of a novel polysaccharide from Tribonema minus and preparation of polysaccharide–selenium nanoparticles with enhanced antioxidant activity

Isolation and structure elucidation of cell surface polysaccharides from Oenococcus oeni.

Isolation and structure elucidation of cell surface polysaccharides from Oenococcus oeni.

Isolation, structural characterization, and antiviral activity of a sulfated polysaccharide (LJP-1) from Laminaria japonica.

Isolation, structural characterization, and antiviral activity of a sulfated polysaccharide (LJP-1) from Laminaria japonica.

Potential Pharmacological Ability of a Novel Marine Polysaccharide Extracted from Tunisian Green Algae Chaetomorpha aerea to Accelerate Dermal Wound Healing in Rats.

Bacterial infection and oxidative stress generation are significant obstacles to dermal wound healing. The present study undertakes the isolation of a sulfated polysaccharide from the Tunisian green algal "Chaetomorpha aerea" named PCA. The polysaccharide PCA was structurally characterized using Fourier Transformed Infrared (FT-IR), and monosaccharide analysis was carried out by HPLC-FID X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). The antioxidant potential of polysaccharides extracted from the Chaetomorpha area was evaluated in vitro using various antioxidant assays, and the antibacterial activity of PCA against four Gram-negative bacteria was estimated. The wound healing capacity of PCA was evaluated in vivo using an excision wound model in rats. FT-IR spectra revealed the characteristic bands of polysaccharides. HPLC-FID revealed a heteropolysaccharide composed of arabinose, glucose, glucuronic acid, and galactose units. Indeed, the X-ray diffraction revealed a semi-crystalline structure of PCA. The obtained data showed a strong antioxidant capacity and an interesting antibacterial activity against four-gram negative bacteria Escherichia coli, Acinetobacter baumannii, Klebsiella pneumonia, and Pseudomonas aeruginosa. These biological data strongly support the beneficial effects of PCA in accelerating wound healing in rats. The in vivo study on rats demonstrated that PCA significantly accelerated the wound healing process over an 11-day treatment period. The application of PCA on wounds led to enhanced collagen fiber synthesis, as evidenced by histological staining, which showed increased collagen deposition at the wound site. Additionally, PCA treatment resulted in faster wound closure, with measurements showing a marked reduction in wound size compared to control groups. The present study highlights the promising pharmacological effects of PCA, suggesting its potential application in wound dressings due to its robust antioxidant, antibacterial, and wound-healing properties.

Immunostimulating and Anticancer Activities of the Pectic Polysaccharide from Panax ginseng Leaves Treated with High Pressure/Enzyme Process

This study was designed to investigate the immunostimulatory and anticancer efficacies of pectic polysaccharides from ginseng leaves treated using the high-pressure extraction method (HPEM). The isolation of polysaccharides using HPEM resulted in 1.35-fold higher polysaccharide yields than those obtained using the commonly used hot water extraction method. In addition, component sugar analysis of ginseng-leaf-derived polysaccharides (GLHP) showed the presence of nine different types of monosaccharides, including galacturonic acid, galactose, rhamnose, and arabinose, which are characteristic of pectic polysaccharides. In addition, GLHP effectively induced activation of the complement system, and macrophages stimulated with GLHP showed enhanced production of cytokines such as IL-6, IL-12, and TNF-α. Intravenous (i.v.) and oral administration (p.o.) of GLHP significantly increased the cancer-cell-killing ability of spleen-derived NK cells. In a lung-cancer-bearing mouse model using Colon26-M3.1 carcinoma, prophylactic i.v. and p.o. GLHP potently inhibited 95.2% and 33.5% of lung cancer, respectively. Furthermore, GLHP showed significant anticancer effects, even in mice with NK cell dysfunction, via the anti-asialo GM1 antibody. These effects may be related to the cancer-cell-killing effects of cytotoxic T lymphocytes (CTL). Therefore, GLHP, a polysaccharide isolated from ginseng leaves using HPEM, has a potent anticancer effect, and these effects are closely related to the stimulation of various immune factors.

Isolation and characterisation of a heparosan capsular polysaccharide and a core oligosaccharide from Moraxella lincolnii strain CCUG 52988.

Isolation and characterisation of a heparosan capsular polysaccharide and a core oligosaccharide from Moraxella lincolnii strain CCUG 52988.

Marine Algae Polysaccharides: An Overview of Characterization Techniques for Structural and Molecular Elucidation.

Polysaccharides make up a large portion of the organic material from and in marine organisms. However, their structural characterization is often overlooked due to their complexity. With many high-value applications and unique bioactivities resulting from the polysaccharides' complex and heterogeneous structures, dedicated analytical efforts become important to achieve structural elucidation. Because algae represent the largest marine resource of polysaccharides, the majority of the discussion is focused on well-known algae-based hydrocolloid polymers. The native environment of marine polysaccharides presents challenges to many conventional analytical techniques necessitating novel methodologies. We aim to deliver a review of the current state of the art in polysaccharide characterization, focused on capabilities as well as limitations in the context of marine environments. This review covers the extraction and isolation of marine polysaccharides, in addition to characterizations from monosaccharides to secondary and tertiary structures, highlighting a suite of analytical techniques.

Isolation and characterization of extracellular polysaccharide from Colletotrichum gloeosporioides: New plant immune elicitor for plant protection.

Isolation and characterization of extracellular polysaccharide from Colletotrichum gloeosporioides: New plant immune elicitor for plant protection.

Isolation, purification, structural characterization, and antitumor activity of Gynura divaricata polysaccharides.

Isolation, purification, structural characterization, and antitumor activity of Gynura divaricata polysaccharides.

Isolation, structural characterization, and hypoglycemic activity of polysaccharides from the stems of Panax ginseng C. A. Meyer

The purpose of this study was to obtain polysaccharides from Panax ginseng C. A. Meyer stems (PGSPs), agro-byproducts with development potential, and fully explore the potential value in P. ginseng stems. Two novel polysaccharides firstly from P. ginseng stems (PGSP-1 and PGSP-2) were obtained by water extraction and alcohol precipitation method and column chromatography, and then characterized by FT-IR, HPGPC, HPLC, SEM, TGA, GC–MS and NMR. The results demonstrated that PGSP-1 (Mw = 723.83 kDa) and PGSP-2 (Mw = 620.48 kDa) were characterized →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1 → and →2)-α-L-Rhap-(1 → as the skeleton, →4,6)-β-D-Galp-(1 → and →2,6)-α-D-Manp-(1 → as the cross junction, α-L-Araf-(1 → as the terminal unit, and PGSP-1 still contained →4)-β-D-Galp, while PGSP-2 contained →4)-β-D-Xylp-(1→, →3)-β-D-GlcpA-(1→, →4)-β-D-GalpA-(1 →) and α-D-Glcp-(1→, with different microstructures and thermal stability. And the results of hypoglycemic activity revealed that both PGSP-1 and PGSP-2 showed excellent inhibitory activity against α-glucosidase and α-amylase, in which PGSP-2 had the better performance. The inhibition kinetics result showed that PGSPs on α-glucosidase and α-amylase were non-competitive type and mixed type inhibition. This study provided a theoretical basis for making full use of and exploiting the economic value of agro-byproducts such as P. ginseng stems and offered a theoretical reference for the effective utilization of PGSP as a functional component to prevent and reduce T2DM.

Isolation, Characterization, Moisturization and Anti-HepG2 Cell Activities of a Novel Polysaccharide from Cyanobacterium aponinum.

Polysaccharides from cyanobacteria are extensively reported for their complex structures, good biocompatibility, and diverse bioactivities, but only a few cyanobacterial species have been exploited for the biotechnological production of polysaccharides. According to our previous study, the newly isolated marine cyanobacterium Cyanobacterium aponinum SCSIO-45682 was a good candidate for polysaccharide production. This work provided a systematic study of the extraction optimization, isolation, structural characterization, and bioactivity evaluation of polysaccharides from C. aponinum SCSIO-45682. Results showed that the crude polysaccharide yield of C. aponinum reached 17.02% by hot water extraction. The crude polysaccharides showed a porous and fibrous structure, as well as good moisture absorption and retention capacities comparable to that of sodium alginate. A homogeneous polysaccharide (Cyanobacterium aponinum polysaccharide, CAP) was obtained after cellulose DEAE-52 column and Sephadex G-100 column purification. CAP possessed a high molecular weight of 4596.64 kDa. It was mainly composed of fucose, galactose, and galacturonic acid, with a molar ratio of 15.27:11.39:8.64. The uronic acid content and sulfate content of CAP was 12.96% and 18.06%, respectively. Furthermore, CAP showed an in vitro growth inhibition effect on human hepatocellular carcinoma (HepG2) cells. The above results indicated the potential of polysaccharides from the marine cyanobacterium C. aponinum SCSIO-45682 as a moisturizer and anticancer addictive applied in cosmetical and pharmaceutical industries.

Isolation, purification, and structural elucidation of a water-soluble polysaccharide derived from Phellinus baumii Pilát mycelia

Isolation, purification, and structural elucidation of a water-soluble polysaccharide derived from Phellinus baumii Pilát mycelia

Isolation, physico-chemical characteristics and acute toxicity determination of water-soluble polysaccharide from basidial raw material Ganoderma lucidum

As a result of the study, branched polysaccharides were isolated from the basidiomycete raw materials of Ganoderma lucidum. It has been established that the isolated fractions contain branched polysaccharides in the form of complexes with melanin. After purification of polysaccharides by ion-exchange chromatography, two fractions were obtained from basidial raw materials: neutral polysaccharides GW-1 with a yield of 25.71% and anionic polysaccharides GW-2, the yield of which was 5.26%, respectively. The physicochemical properties of the obtained samples were studied by IR and UV spectroscopy. The degree of purity of the obtained fractions of branched polysaccharides was established. Using gas chromatography, one-dimensional (13C NMR, 1H NMR) and two-dimensional (COSY, TOCSY, HSQC, HMBC, NOESY) NMR spectroscopy, the composition and molecular structure of the obtained polysaccharide samples were determined. The results showed that the isolated and purified polysaccharides are branched glucans with 1,4,6- and 1,3,6-bonds between glucopyranose units. Pharmacotoxicological studies were carried out on white outbred mice and it was found that the resulting polysaccharides belong to class V, practically non-toxic compounds (LD50 ≥ 2000 mg/kg). Isolated polysaccharides (GW) are promising biologically active components, on the basis of which it is possible to create drugs with hepatoprotective and antitumor activity.

ИЗУЧЕНИЕ ПОЛИСАХАРИДОВ ПОБЕГОВ И ЛИСТЬЕВ СМОРОДИНЫ ЧЕРНОЙ

Medicinal plants occupy a special place among the sources of pharmaceutical substances. Long-term experience of folk, traditional and official medicine of different countries confirms the effectiveness of the use of medicines of plant origin for the prevention and treatment of various diseases. Advantages of their use in comparison with the products of chemical synthesis, determine the relevance of expanding the nomenclature of currently used medicines by means of studying new plant species. Black currant shoots, as easily available for harvesting raw materials, are of interest for study in order to establish the possibility of their use in scientific medicine, so at the Department of Pharmacognosy FGBOU VO "PGFA" Ministry of Health of Russia conducted studies of shoots and leaves of black currant. The article presents the results of isolation and chemical study of polysaccharides of shoots and leaves of black currant wild and cultivated by paper chromatography and spectrophotometry. It is established that this carbohydrate complex is represented by water-soluble polysaccharides (WPS), pectin substances (PS), hemicelluloses (HC). The qualitative composition of monosaccharides by fractions was established. The presence of galactose, glucose, fructose, arabinose, rhamnose in fractions of GRPS and SP and glucose and xylose in HC was proved. The quantitative content of polysaccharide fractions was studied. The predominant fraction in the polysaccharide complex of shoots and leaves of black currant is pectin substances.

Isolation and characterization of a hepatoprotective polysaccharide from Lonicera caerulea L. var. edulis Turcz. ex Herd. fruit against APAP-induced acute liver injury mice

Isolation and characterization of a hepatoprotective polysaccharide from Lonicera caerulea L. var. edulis Turcz. ex Herd. fruit against APAP-induced acute liver injury mice

Preparation, structural characterization and protective effect on PC12 cells of polysaccharides from the stipe of Morchella esculenta

Preparation, structural characterization and protective effect on PC12 cells of polysaccharides from the stipe of Morchella esculenta

Isolation and Free Radical Scavenging Ability of Linear Polysaccharides From Cuttlebone of Sepia prashadi.

Background This study aimed to isolate linear polysaccharides from Sepia prashadi cuttlebone with the objective of evaluating their ability to scavenge free radicals. By providing new natural components for pharmaceutical and functional food uses, this research advances our understanding of the potential health benefits of polysaccharides originating from marine sources and their antioxidant properties. Objective The objective of the study is to isolate a linear polysaccharide chitosan from Sepia prashadi cuttlebone(produced by the partial deacetylation of chitin),characterize its structure using fourier transform infrared spectroscopy (FTIR),scanning electron microscopy (SEM), and X-ray diffraction (XRD), and explore the isolated polysaccharide's free radical scavenging potential. Material and methods Linear polysaccharide, chitosan was extracted chemically from Sepia prashadifrom cuttlebone waste, by demineralization and deproteinization.Chemical characterization of chitosan was performed using Fourier transform infrared spectroscopy (FTIR) in the 400-4000 nm frequency range. The surface characteristics of chitosan, such as its texture, porosity, and roughness, are visible in scanning electron microscopy (SEM) images. X-ray diffraction (XRD) canbe utilized to examine how chitosan interacts with other substances, such as medications or nanoparticles, by analyzing alterations in thediffraction pattern during complexation or formulation. Scavenging ability was demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide radical, and chelating ability of ferrous ions assays. Results Chitosan is formed from chitin. The extraction yields of chitosan and chitin were 78% and 39%, respectively. High levels of superoxide radical scavenging activity (76.1%), DPPH radical scavenging activity (62.1%) and chelating activity (127.5% at 100 g/mL) were observed in cuttlebone chitosan. Sepia prashadi showed an increased antioxidant activity in chitosan. Conclusion The goal of this study was to determine the effectiveness of various extraction techniques for preserving the antioxidant activity of chitosan derived from Sepia prashadicuttlebone waste. The maximum scavenging activity was demonstrated by both the chelating ability and antioxidant activity. Considering that this raw material is derived from renewable resources and produces highly valued chemicals, it is a profitable endeavor.

The isolation, structure characterizations and anti-photoaging activities of sulfated polysaccharides isolated from Sargassum fusiforme

BackgroundOur previous study found that crude polysaccharide extracted from Sargassum fusiforme (PSF) exhibited excellent anti-photoaging activity, but the composition responsible for this activity remained unknown.ResultsIn this study, three fractions (P1, P2 and P3) obtained by hot water extraction and column chromatographic separation from PSF were structurally characterized. The anti-photoaging activities of these isolated polysaccharides were evaluated using HaCaT cells. The results showed that P1, P2 and P3 were acidic polysaccharides with 42.94%, 40.75% and 2.21% uronic acid. In addition, the contents of sulfate groups were 3.01%, 10.05% and 7.44%, respectively. Moreover, their content of reducing sugar was low, and none of the fractions had a triple-stranded helical structure. P1 had the largest average molecular weight of 208.89 kDa, while P2 and P3 had a relatively lower molecular weight of 154.09 and 151.71 kDa, respectively. Treatment with P2 effectively safeguarded the viability of HaCaT cells exposed to UVB irradiation. Additionally, it inhibited the secretion of MMP-1 and MMP-3 while elevating the content of hydroxyproline (HYP) in the cell supernatant. Methylation and NMR analysis suggested the main structure of P2 might be → [4)-β-ManA-(1]23 → 4)-β-ManA-(1 → 3)-β-d-Glcp-(1 → 4)-β-d-Glcp-(1 → 4)-β-d-Glcp-(1 → . The two terminal groups of β-ManA-(1 → and β-d-Glcp-(1 → were connected via the glycosidic bonds of → 3, 4)-O-3 in β-ManA-(1 → and O-6 in → 4, 6)-β-d-Glcp-(1 → , respectively.ConclusionsThese findings will open new avenues for exploring the potential use of Sargassum fusiforme polysaccharide, particularly P2, as a functional ingredient with anti-photoaging activity.Graphical

Isolation, structural characterization and biological activities of polysaccharides from Chondrus crispus

Isolation, structural characterization and biological activities of polysaccharides from Chondrus crispus

Isolation, purification, and biological activities of polysaccharides from Amorpha fruticosa flowers

The extraction, isolation, structural characterisation and biological activities of polysaccharides from Amorpha fruticosa flowers were investigated. First, the crude polysaccharide AFP was extracted, and two major purified polysaccharide fractions AFP-2 and AFP-3 were isolated. The molecular weight and monosaccharide compositions of AFP-2 and AFP-3 were determined. Then the antioxidant activities of AFP, AFP-2 and AFP-3 were assessed by DPPH radical, β-Carotene bleaching and hydroxyl radical assays. All three tested polysaccharides showed good antioxidant activity while AFP was the strongest one. The study also showed that AFP, AFP-2 and AFP-3 have good tyrosinase inhibition, moisture absorption and retention activities. The results will provide a helpful reference for the application of polysaccharide from Amorpha fruticosa flowers as a natural cosmetic ingredient.