Isolation Of Flavonoid Compounds Research Articles

Isolation of Flavonoids Compounds from Kesumba Keling Leaves (Bixa orellana L.)

Isolation of flavonoid compounds from leaves of Kesumba Keling (Bixa orellana L.). Extraction has been done with maceration by methanol solvent. The concentrated extract of methanol was added with ethyl acetate. The concentrated ethyl acetate extract was then dissolved with methanol and partitioned with n-hexane. The concentrated methanol extract was acidified by 6% HCl, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with eluent n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/ v). The compounds were purified with TLC preparative yielding yellow gum weighing 29.7 mg with Rf=0.51. The compound was further identified by Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR). Spectroscopic data show that the compound was isoflavone.

Isolation, Identification, and Antioxidant Activity of Flavonoid Compounds in the Ethanol Extract in Bandotan Leaves (Ageratum conyzoides)

Isolation of flavonoid compounds from the ethanol extract of Bandotan leaves (Ageratum conyzoides L.) has been successfully conducted. The structure of flavonoid isolates was identified using UV-Vis spectrophotometry with the addition of shear reagents and FTIR. In addition, the antioxidant activity of the isolates was determined and studied using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) method. This study aimed to isolate, identify, and test the antioxidant activity of flavonoid compounds from Bandotan leaves. An antioxidant activity test was carried out on ethanol extract, and flavonoid isolates using the DPPH method. Based on this research, Bandotan leaf ethanol extract yielded 13.964%. The results of the phytochemical screening test revealed that the leaf powder and ethanol extract of Bandotan leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, and triterpenoids. The total flavonoid content of the ethanol extract of Bandotan leaves was 129.27 mg EQ/g extract. Identification of flavonoid isolates using a UV-Vis spectrophotometer showed that flavonoid isolates belong to the flavanone compound class with maximum absorption at a wavelength of 315 nm (band I) and 280 nm (band II). FTIR analysis showed that the flavonoid isolates had functional groups O-H, C-H aromatics, C-H alkanes, C=O, C=C aromatics, C-O ethers, C-O alcohols, and substituted aromatic rings. Identification of the structure of flavonoid isolates with a spectrophotometer with the addition of shear reagent and FTIR, presumably that the isolate was a 4’-hydroxy flavanone compound. The antioxidant activity of ethanol extract showed moderate antioxidant activity (IC50 118.19 µg/mL) and was classified as very weak (IC50 1185.5 µg/mL).

Isolation of Flavonoid Compounds from Salam (Syzygium polyantha Wight) Leaves

Isolation of flavonoid compounds from Salam (Syzygium polyantha Wight) leaves has been done by maceration technique with methanol solvent. Methanol extract obtained was evaporated and dissolved with ethyl acetate. The solution was then concentrated and evaporated. The ethyl acetate fraction was dissolved with methanol and partitioned with n-hexane solvent. The methanol layer was evaporated and then tested with Benedict’s reagent and then acidified with HCl 6% while heated. It was then extracted partition with chloroform. The chloroform layer was separated using Column Chromatography with silica gel as the stationary phase and n-hexane: ethyl acetate 90:10, 80:20, 70:30, and 60:40 v/v, respectively as the mobile phase. The pure compound was paste, brownish red, mass = 8.5 mg, and Rf=0.60. It was a positive reaction with flavonoid compound reagents. The compound was further identified by using UV-Visible, FT-IR, and 1H-NMR spectroscopy. Spectroscopy data obtained indicated that the compound is a flavonoid.

Isolation of Flavonoid Compounds from Suren Leaves (Toona sureni)

Isolation of flavonoid compound from Suren leaves (Toona sureni) has been done using the extraction method. The suren leaves extract was separated by using the column chromatography method. The isolated compound results were yellow paste with weight 47 from fraction 55-67 and Rf = 0.44 of eluent n-hexane: ethylacetate 60:40 (v/v). The identification process was analyzed by UV-Vis, FT-IR spectroscopy, and 1H-NMR spectroscopy. The obtained result shows that the isolated compound was isoflavone.

Isolation of Flavonoid Compounds from Bangun-bangun Leaves (Plectranthus amboinicus (Lour.) Spreng.)

Isolation of flavonoid compounds from leaves of Bangun-bangun (Plectranthus amboinicus (Lour.) Spreng.) has been conducted by maceration process with methanol solvent, added with ethyl acetate, and then partially extracted with n-hexane, acided by HCl 6% and partition extracted with chloroform. The concentrated extract of chloroform was separated using column chromatography with eluent n-hexane: ethyl acetate. The resulted tawny paste yielding was 10 mg with Rf= 0.38. The resulted compounds were characterized using UV-Vis, FT-IR, and 1H-NMR which was estimated as a flavonoid is a flavanone.

Isolasi Dan Karakterisasi Senyawa Flavonoid Dari Kulit Akar Tumbuhan Sukun Artocarpus Altilis (Parkinson) Fosberg

Isolation of flavonoid compounds from Plants Roots Leather Breadfruit (Artocarpus altilis (Parkinson) Fosberg) that grow in the village of Banjar District State Tanggamus Wonosobo regency of Lampung Province has been conducted. Isolation of flavonoid compounds is done by maceration method using the solvent ethyl acetate. Stages of purification of samples carried out by chromatographic methods include vacuum liquid chromatography (VLC), thin layer chromatography (TLC), gravity column chromatography (GCC), and flash chromatography. As for the characterization of flavonoid compounds used infrared spectroscopy (IR), ultaviolet-visible spectroscopy (UV-Vis), nuclear magnetic resonance spectroscopy (NMR), and test the melting point. Based on the results of research that has been done, pure flavonoid compounds obtained amorphous form of yellow crystals with a melting point of 247,5-249 0C. Based on the analysis of ultraviolet-visible spectroscopy, infrared, and nuclear magnetic resonance can be concluded that the isolation of these compounds is artonin E.

Isolation and Antimicrobial Activity of Flavonoid Compounds from Mahagony Seeds (Swietenia macrophylla, King)

Flavonoid is one of the secondary metabolites compounds in mahogany seeds. Mahogany seeds can be used as an antimicrobial. This study aims to determine the antimicrobial activity of flavonoid compounds from mahogany seeds against Escherichia coli (E.coli) and Bacillus cereus (B.cereus). Isolation of flavonoid compounds done step by step. First, the maceration using n-hexane, then with methanol. The methanol extract was dissolved in ethyl acetate and aquadest, then separated. Ethyl acetate extract evaporated Flavonoid compounds were. The testing of antimicrobial activity of flavonoid compounds using the absorption method. The results showed that the antimicrobial activity of flavonoid compounds from mahogany seeds shows the inhibitory activity and provide clear zone against bacteria E.coli with value Inhibitory Regional Diameter 18.50 mm respectively, and 14.50 mm to the bacteria. Based on the results of the study, it can be concluded that flavonoid compounds from mahogany seeds have antimicrobial activity against E.coli and B.cereus.

English

This paper is reports on the isolation of flavonoid compounds from two medicinal plant species that are widely used in Southern Africa, Elephantorrhiza elephantina and Pentanisia prunelloides which belong to families Fabaceae and Rubiaceae, respectively. The identification and comparative analysis of flavonoids in E. elephantina and P. prunelloides as well as the structural elucidation of flavonoids were performed using liquid-chromatography electron-spray ionization mass spectroscopy (LC-ESI-MS) and spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Among the flavonoids identified include epigallocatechin gallate (EGCg), epicatechin (EC), quercetin, epicatechin gallate (ECg), kaempferol and their polymeric derivatives. The analysis revealed that, P. prunelloides contain more flavonoids (0.81%) than E. elephantina (0.77%). 2,2-Diphenylpicrylhydrazyl (DPPH) radical antioxidant tests confirmed the presence of these compounds. Key words: Flavonoids, Fabaceae, Rubiaceae, liquid-chromatography electron-spray ionization mass spectroscopy (LC-ESI-MS).