The secondary electron energy-dependent partial differential ionization cross sections at fixed incident electron energies 100 and 200 eV and their integral or total ionization cross sections in the incident electron energy range ionization threshold to 5 keV for C5H5N Bio-molecule due to electron impact corresponding to the produced major cations are calculated by employing revisited Jain-Khare semiempirical approach. The averaged secondary electron energy and the ionization rate coefficients are calculated from the ionization threshold to 5 keV. The present results are compared with available experimental and theoretical results and are found to be in satisfactory agreement.