Ionic Strength Of Sample Solution Research Articles

Facile synthesis of magnetic layered double hydroxide/metal–organic framework MIL-100(Fe) for simultaneous extraction and enantiomeric determination of three chiral triazole fungicides in water and juice samples

For the first time, magnetic layered double hydroxide/metal–organic framework (Fe3O4@CuZnAl-LDH@MIL-100(Fe)) was successfully fabricated and applied for enrichment of triazole fungicides from water and juice samples prior to chiral HPLC-UV analysis. Several key parameters influencing the extraction efficiency including extraction time, desorption time, sample solution pH, the ionic strength of sample solution, adsorbent amount, desorption volume, desorption solvent and sample volume were investigated by Plackett-Burman design and Box-Behnken design. Under the optimum conditions, the limit of detections (LODs) and limit of quantifications (LOQs) were in the ranges of 0.35 to 1.20 μg L−1 and from 1.00 to 3.80 μg L−1, respectively. The average spiked recoveries and enrichment factors ranged from 86.28-98.22 % and 21.6–23.8, respectively. The linearity was in the range of (5–1000 μg L−1) with satisfactory correlation coefficients (R2) values (0.9987–0.9999) and a good precision (RSDs ≤ 5.74 %). Moreover, the computational simulation was first employed to analyze adsorption properties of MIL-100(Fe) against triazole fungicides. This work provides a promising method for trace chiral pesticide analysis in aqueous samples.

Optimized three‐phase hollow fiber liquid‐phase automated microextraction and its application in ultra‐trace analysis of three pyrethroids in water and fruit samples using high‐performance liquid chromatography‐diode array detection

AbstractIn the present manuscript, an automated three‐phase hollow fiber liquid‐phase microextraction followed by high‐performance liquid chromatography‐diode array detection is applied for the extraction and determination of three pyrethroids, deltamethrin, fenpropathrin, and permethrin in water and fruit samples. N‐dodecane was selected as a supported liquid membrane (SLM) and its polarity was adjusted by trioctylphosphine oxide one‐variable‐at‐a‐time method was used in order to achieve optimal hollow fiber liquid‐phase microextraction parameters such as type of organic acceptor phase, the composition of SLM, ionic strength of sample solution, extraction time, length of hollow fibers and stirring speed. Under the optimal conditions, the calibration curves for the three pyrethroids were plotted, and figures of merit of the proposed method were calculated. The linear dynamic ranges were in the range of 0.9–200, 0.75–200, and 1.5–200 μg/L for deltamethrin, fenpropathrin, and permethrin, with the coefficient of determination better than 0.998. The limits of detection were in the range of 0.3, 0.25, and 0.5 and the limits of quantification were in the range of 0.9, 0.75, and 1.5 μg/L for deltamethrin, fenpropathrin, and permethrin, respectively. The precision of the method was evaluated in terms of relative standard deviation at two different concentration levels 10 and 50 μg/L. The results of real sample analysis in cucumber exhibited high sensitivity, excellent sample clean‐up, favorable repeatability, and suitable accuracy.

Electrospun UiO-66-F4/polyacrylonitrile nanofibers for efficient extraction of perfluoroalkyl and polyfluoroalkyl substances in environmental media

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a family of emerging contaminants which are widely present in environment. In this work, novel UiO-66-F4/polyacrylonitrile (UiO-66-F4/PAN) hybrid nanofibers were firstly prepared via blend electrospinning or in-situ growth method for the pipette-tip solid phase extraction of PFASs. Characterizations demonstrate the as-synthesized UiO-66-F4/PAN nanofibers have good chemical and thermal stability, possess large surface area (248 m²/g) and mesoporous framework structure. Several extraction factors including the amount of adsorbent, pH and ionic strength of sample solution, extraction time and eluent were investigated and the optimum conditions are 20 mg of the selected sorbent, adjusting to pH 5 and adding 4% w/v NaCl to sample solution, extraction for 12 min (3 min × 4). The good adsorption affinity of UiO-66-F4/PAN for PFASs can be attributed to the extensive adsorption sites and multiple interactions including hydrophobic interaction, hydrogen bonding and F-F interaction. Low limit of detection (0.008–0.076 µg/L), limit of quantification (0.010–0.163 µg/L) and recoveries (70.84–113.57%) for 9 PFASs with relative standard deviations < 15% were achieved. When applied in the analysis of target PFASs in lake water, tap water, beverage, and shrimp muscle samples, this method could achieve robust and accurate results with sufficient sensitivity for nine PFASs.

Dispersive liquid-liquid microextraction based on a new hydrophobic deep eutectic solvent for the determination of phenolic compounds in environmental water samples.

Dispersive liquid-liquid microextraction has garnered increasing attention in sample preparation due to its rapid and efficient extraction process. In this study, a new terpineol-based hydrophobic deep eutectic solvent was firstly synthesized by mixing α-terpineol with 1-octanoic acid, and then applied to analysis of phenols from water samples by dispersive liquid-liquid microextraction combined with high-performance liquid chromatography and diode array detection. Infrared spectroscopy indicated that hydrogen bonding was responsible for the formation of deep eutectic solvent between α-terpineol and 1-octanoic acid. After optimization of several parameters, such as the type and volume of deep eutectic solvent and the disperser, pH and ionic strength of sample solution, the developed method exhibited excellent extraction performance to the phenols with the enrichment factors from 27 to 32. Good linearity was acquired ranging from 5 to 5000μg/L, and detection of limits of the proposed method for the phenols ranged from 0.15 to 0.38μg/L. The recoveries measured by spiked samples at three concentration levels ranged from 81.6 to 99.3%, and precision was found with intra- and inter-day relative standard deviations less than 8.7 and 9.2%, respectively. Finally, the proposed method was successfully applied to the determination of the phenols in environmental water samples.

Preparation of a nano-biocomposite film based on halloysite-chitosan as the sorbent for thin film microextraction

This work introduced an environmentally friendly sorbent based on halloysite-chitosan nano-biocomposite for thin film microextraction (TFME). Chitosan as a biodegradable and biocompatible polymer was used to prepare thin film sorbent. To improve the mechanical and chemical resistance of the film and increasing the adsorption efficiency, halloysite nanotubes were combined with the chitosan to prepare the thin film sorbent. The film was used for TFME of 17α-ethynylestradiol followed by high-performance liquid chromatography-fluorescence detection. Effective parameters such as the amount of chitosan and halloysite, ionic strength of sample solution, type and volume of desorption solvent, desorption and extraction times were optimized. The linear dynamic range of the method was in the range of 1.0–50.0 μg L−1. The detection limit and relative standard deviation of the method were 0.4 μg L−1 and 9%, respectively. The method was validated for the TFME of 17α-ethynylestradiol in river water, wastewater, and tap water samples, and recoveries were found to be in the range of 67–106%.

Purification and enrichment of polycyclic aromatic hydrocarbons in environmental water samples by column clean-up coupled with continuous flow single drop microextraction

A novel method for the determination of sixteen polycyclic aromatic hydrocarbons (PAHs) in environmental water samples was developed using column clean-up (CCU) coupled with continuous flow single drop microextraction (CF-SDME) prior to the determination by gas chromatography-mass spectrometry (GC–MS). In this method, purification, extraction and enrichment were carried out in a single step, which reduced the effect of most organic interferents on the determination of target analytes, greatly simplified the operation process and shortened the whole pre-treatment time. The CCU-CF-SDME system was first used, and shortcomings such as long extraction time, instability of suspended microdrop of the traditional SDME system were overcome. The influences of several experimental parameters, including the type and amount of column packing material, the type and volume of extraction solvent, the flow rate of sample solution, extraction temperature, pH and ionic strength of sample solution, on the extraction efficiency were investigated. Under the optimal experimental conditions, the good linearities were obtained in the concentration range of 0.02–10 μg L−1 with correlation coefficients ranging from 0.9912 to 0.9995 for all the analytes. The limits of detection and quantification of the analytes were in the range of 0.0012–0.0101 μg L−1 and 0.0041–0.0336 μg L−1, respectively. The pre-concentration factors in the range of 777–978 for the PAHs were obtained. The proposed method was applied on the analysis of environmental water samples, and the recoveries of target analytes were in the range of 81.8–105.8% with the relative standard deviations ranging from 0.5% to 6.4%. The results showed that the developed method was a simple, quick and feasible method for the determination of PAHs in environmental water samples.

Determination of carboxylic acids in non-alcoholic beer samples by an ultrasonic-assisted dispersive micro-solid phase extraction based on Ni/Cu-Al layered double hydroxide nanocomposites followed by gas chromatography

Magnetically separable layered double hydroxide Ni/CuAl-LDH nanocomposites were synthesized and employed as ultrasonic-assisted dispersive micro-solid phase extraction (UA-D-μSPE) sorbent to extract several carboxylic acids (namely propionic, butyric, pentanoic, hexanoic, heptanoic, octanoic, and decanoic) from non-alcoholic beer samples. Ni/CuAl-LDH sorbent was characterized by Fourier transform-infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). Effective variables such as amount of sorbent (mg), pH and ionic strength of sample solution, volume of eluent solvent (μL), vortex, and ultrasonic times (min) were investigated via fractional factorial design (FFD). The significant variables were optimized by a Box–Behnken design and combined by a desirability function (DF). Under optimized conditions, the calibration graphs of analytes were linear in a concentration range of 0.05–100μg/mL and had correlation coefficients more than 0.997. The limits of detection and quantification were in the ranges of 16–40μg/L and 53–133μg/L, respectively. This procedure was successfully employed in the determination of target analytes in spiked beer samples, and the relative mean recoveries ranged from 87 to 110%.

Extraction of phenolic compounds from water samples by dispersive micro-solid-phase extraction.

In this article, the use of magnetically separable sorbent polyaniline/silica-coated nickel nanoparticles is evaluated under a dispersive micro-solid-phase extraction approach for the extraction of phenolic compounds from water samples. The sorbent was prepared by in situ chemical polymerization of aniline on the surface of silica-modified nickel nanoparticles and was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray powder diffraction, scanning electron microscopy, energy-dispersive X-ray spectrometry, and vibrating sample magnetometry. Effective variables such as amount of sorbent (milligrams), pH and ionic strength of sample solution, volume of eluent solvent (microliters), vortex, and ultrasonic times (minutes) were investigated by fractional factorial design. The significant variables optimized by a Box-Behnken design were combined by a desirability function. Under the optimized conditions, the calibration graphs of analytes were linear in a concentration range of 0.02-100 μg/mL, and with correlation coefficients more than 0.999. The limits of detection and quantification were in the ranges of 10-23 and 33-77 μg/L, respectively. This procedure was successfully employed in the determination of target analytes in spiked water samples; the relative mean recoveries ranged from 96 to 105%.

Comparison of Headspace-Single Drop Microextraction and Dispersive Liquid–Liquid Microextraction for Determination of Benzene in Juice Drinks Containing Vitamin C

In this paper, a fast and effective headspace-single drop microextraction (HS-SDME) method was applied for the determination of traces of benzene in juice drinks containing vitamin C. Benzene is produced in drinks as a result of interaction of sodium benzoate (as food preservative) and ascorbic acid (vitamin C). A mixture of n-hexadecane and n-dodecane was used as extractant in the form of organic drop. Other parameters affecting the extraction of benzene, including volume of organic drop, time of extraction, volume, pH and ionic strength of sample solution, were studied and optimized. The obtained dynamic range under optimised conditions was from 2 to 350 μg L−1 with the limit of detection of 1.49 μg L−1. The obtained data for HS-SDME of benzene were compared with the data obtained for the determination of benzene with dispersive liquid–liquid microextraction (DLLME). It was observed that though DLLME is simpler and faster, the limit of detection and the repeatability of HS-SDME were better.

Rapid Determination of Trace Sulfonamides in Milk by Graphene Oxide-Based Magnetic Solid Phase Extraction Coupled with HPLC–MS/MS

A simple and rapid method based on magnetic solid-phase extraction (MSPE) combined with high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC–MS/MS) was used for the determination of 15 sulfonamides from milk samples. The extraction and cleanup used a graphene oxide-based magnetic nanocomposite (Fe3O4@GO) as an adsorbent. Various experimental parameters that could affect the extraction efficiencies, such as the amount of Fe3O4@GO, the extraction time, the ionic strength of sample solution, and the type of eluent, were investigated. Under optimized experimental conditions, good linearity was observed in the range of 2.0 to 100.0 μg L−1 for all of the analytes, with correlation coefficients (R2) ranging from 0.994 to 0.999. The limits of detection for the method ranged between 0.02 and 0.13 μg L−1. Mean values of the relative standard deviation of intraday and interday precision ranging from 1.0 to 7.3 % and from 1.7 to 8.1 % were obtained, respectively. The average recoveries were between 73.4 and 97.4 % at three different spiked levels. It was confirmed that the Fe3O4@GO nanocomposite was an effective MSPE material for use in sulfonamide analyses in milk samples.

Preconcentration and determination of chlordiazepoxide and diazepam drugs using dispersive nanomaterial-ultrasound assisted microextraction method followed by high performance liquid chromatography.

Benzodiazepines (BDs) are used widely in clinical practice, due to their multiple pharmacological functions. In this study a dispersive nanomaterial-ultrasound assisted- microextraction (DNUM) method followed by high performance liquid chromatography (HPLC) was used for the preconcentration and determination of chlordiazepoxide and diazepam drugs from urine and plasma samples. Various parameters such as amount of adsorbent (mg: ZnS-AC), pH and ionic strength of sample solution, vortex and ultrasonic time (min), and desorption volume (mL) were investigated by fractional factorial design (FFD) and central composite design (CCD). Regression models and desirability functions (DF) were applied to find the best experimental conditions for providing the maximum extraction recovery (ER). Under the optimal conditions a linear calibration curve were obtained in the range of 0.005-10μgmL(-1) and 0.006-10μgmL(-1) for chlordiazepoxide and diazepam, respectively. To demonstrate the analytical performance, figures of merits of the proposed method in urine and plasma spiked with chlordiazepoxide and diazepam were investigated. The limits of detection of chlordiazepoxide and diazepam in urine and plasma were ranged from 0.0012 to 0.0015μgmL(-1), respectively.

Determination of Rhodamine B in Food Using Ionic Liquid–Coated Multiwalled Carbon Nanotube–Based Ultrasound-Assisted Dispersive Solid-Phase Microextraction Followed by High-Performance Liquid Chromatography

A modified Quick, Easy, Cheap, Effective, Rugged, and Safe method based on ultrasound-assisted dispersive solid-phase microextraction (dSPME) was developed for the determination of rhodamine B (RhB) in food samples. In this study, ionic liquid–coated multiwalled carbon nanotube (IL-MWCNT) was first used as dSPME material for the preconcentration of analyte. Several experimental parameters, including the type of ILs, the ratios of MWCNTs and ILs, amount of sorbent, extraction time, pH and ionic strength of sample solution, and desorption conditions, were evaluated. Under optimal experimental conditions, good linearity was observed in the range of 1.0–100.0 ng/mL with the correlation coefficient of 0.9978. The present method was applied to the analysis of different beverage and chili oil samples, and the recoveries of RhB obtained were in the range of 85.1–96.0 %. The results showed that the proposed method was a rapid, convenient, and feasible method for the determination of RhB in food samples.

Functionalized Multiwalled Carbon Nanotube as Dispersive Solid-Phase Extraction Materials Combined with High-Performance Liquid Chromatography for Thiabendazole Analysis in Environmental and Food Samples

A modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on the dispersive solid-phase extraction (dSPE) combined with high-performance liquid chromatography (HPLC) was developed for the determination of thiabendazole (TBZ) in environmental and food samples. Hydroxyl functionalized multiwalled carbon nanotube (MWCNT-OH) was used as dSPE material for the preconcentration of analyte. Several experimental parameters, including the amount of sorbent, extraction time, the pH and ionic strength of sample solution, and desorption conditions, were evaluated. Under optimal experimental conditions, good linearity was observed in the range of 10–1000 ng mL−1 with the correlation coefficient of 0.9962. The limit of detection and quantification were 2.6 and 8.7 ng mL−1, respectively. The present method was applied to the analysis of different environmental and food samples, and the recoveries of TBZ obtained were in the range of 92.9–103.9 % with the relative standard deviations lower than 6.5 %. The results showed that the proposed method was a rapid, convenient, and feasible method for the determination of TBZ in environmental and food samples.

Solid‐phase microextraction with a graphene‐composite‐coated fiber coupled with GC for the determination of some halogenated aromatic hydrocarbons in water samples

In this work, a graphene composite was coated onto etched stainless-steel wire through a sol-gel technique and it was used as a solid-phase microextraction (SPME) fiber. The prepared fiber was characterized by SEM, which revealed that the fiber had a highly porous structure. The application of the fiber was evaluated through the headspace SPME of five halogenated aromatic hydrocarbons (chlorobenzene, bromobenzene, 1,3-dichlorobenzene, 1,2-dichlorobenzene, and 1,2,4-trichlorobenzene) in water samples followed by GC with flame ionization detection. The main factors influencing the extraction efficiency, including headspace volume, extraction time, extraction temperature, stirring rate, ionic strength of sample solution, and desorption conditions, were studied and optimized. Under the optimum conditions, the linearity of the method ranged from 2.5 to 800.0 μg/L for 1,2,4-trichlorobenzene and from 2.5 to 500.0 μg/L for chlorobenzene, bromobenzene, 1,3-dichlorobenzene, and 1,2-dichlorobenzene, with the correlation coefficients (r) ranging from 0.9962 to 0.9980, respectively. The LODs (S/N= 3) of the method for the analytes were in the range between 0.5 and 1.0 μg/L. The recoveries of the method for the analytes obtained for the spiked water samples at 50.0 and 250.0 μg/L were from 76.0 to 104.0%.

Dispersive nano solid material-ultrasound assisted microextraction as a novel method for extraction and determination of bendiocarb and promecarb: Response surface methodology

A new extraction method, based on Dispersive Nano-Solid material-Ultrasound Assisted Micro-Extraction (DNSUAME), was used for the preconcentration of the bendiocarb and promecarb pesticides in the water samples prior to high performance liquid chromatography (HPLC). The properties of NiZnS nanomaterial loaded on activated carbon (NiZnS-AC) are characterized by FT-IR, TEM, and BET. This novel nanomaterial showed great adsorptive ability towards the bendiocarb and promecarb pesticides. The effective variables such as the amount of adsorbent (mg: NiZnS-AC), the pH and ionic strength of sample solution, the vortex and ultrasonic time (min), the ultrasonic temperature (°C), and desorption volume (mL) are investigated by screening 27–4 experiments of Plackett–Burman (PB) design. The important variables optimized by using a central composite design (CCD) were combined by a desirability function (DF). At optimum conditions, the method has linear response over 0.0033–10µgmL−1 with detection limit between 0.0010 and 0.0015µgmL−1 with relative standard deviations (RSDs) less than 5.5% (n=3). The method has been successfully applied for the determination of the bendiocarb and promecarb pesticides in the water samples.

Rapid analysis of phthalates in beverage and alcoholic samples by multi-walled carbon nanotubes/silica reinforced hollow fibre-solid phase microextraction

A novel procedure based on multi-walled carbon nanotubes (MWCNTs)/silica reinforced hollow fibre solid-phase microextraction combined with gas chromatography–mass spectrometry has been developed to analyse trace phthalate acid esters in beverage and alcoholic samples. Because of their excellent adsorption capability towards hydrophobic compounds, functionalized MWCNTs, acting as solid-phase sorbent, were co-deposited with silica particles in the pores of polypropylene hollow fibre through a layer-by-layer self-assembly technique. The parameters influencing the extraction efficiency, such as pH values and ionic strength of sample solution, extraction time, temperature and desorption solvent were optimised. Recoveries for phthalates at spiking levels in different matrices were satisfactory (between 68% and 115%). Moreover, the results were further confirmed by comparing them with those obtained using a solvent extraction method according to the national standard of China.

Magnetic microsphere-confined graphene for the extraction of polycyclic aromatic hydrocarbons from environmental water samples coupled with high performance liquid chromatography–fluorescence analysis

In this paper, a magnetic microsphere-confined graphene adsorbent (Fe3O4@SiO2-G) was fabricated and used for the extraction of five polycyclic aromatic hydrocarbons (fluorene, anthracene, phenanthrene, fluoranthene and pyrene) from environmental water samples prior to high performance liquid chromatography with fluorescence detection. The Fe3O4@SiO2-G was characterized by various instrumental methods. Various experimental parameters that could affect the extraction efficiencies, such as the amount of Fe3O4@SiO2-G, the pH and ionic strength of sample solution, the extraction time and the desorption conditions, were investigated. Due to the high surface area and excellent adsorption capacity of the Fe3O4@SiO2-G, satisfactory extraction can be achieved with only 15mg of the adsorbent per 250mL solution and 5min extraction. Under the optimum conditions, a linear response was observed in the concentration range of 5–1500ngL−1 for fluorene, 2.5–1500ngL−1 for anthracene and 15–1500ngL−1 for phenanthrene, fluoranthene and pyrene, with the correlation coefficients (r) ranging from 0.9897 to 0.9961. The limits of detection (S/N=3) of the method were between 0.5 and 5.0ngL−1. The relative standard deviations (RSDs) were less than 5.6%. The recoveries of the method were in the range between 83.2% and 108.2%. The results indicated that this graphene-based magnetic nanocomposite had a great adsorptive ability toward the five polycyclic aromatic hydrocarbons from environmental water samples.

Characterization of stability of polypeptides and glycoproteins by capillary electrophoresis

A capillary electrophoresis (CE) method was developed for the stability characterization of polypeptides and glycoproteins. Angiotensin II (Ang II), phytagglutinin (PHA), bovine thrombin (B-Thr), human thrombin (H-Thr) and horseradish peroxidase (HRP) were used as polypeptide and glycoprotein mode molecules. The parameters affecting the analysis efficiency for Ang II, such as sample concentration, running buffer, pH and ionic strength of sample solution were optimized, as for the glycoprotein, capillary conditions, charge state of sample, running buffer and applied voltage were optimized. It showed that the Ang II was stable when kept in borate buffer (0.02 mol/L pH 7.4) at 4 degrees C for 48 h. The four glycoproteins were quite stable in borate buffer (0.2 mol/L pH 7.4) at 20, 4, -20 degrees C for 48 h, and also kept stable at - 20 degrees C when deposited over one week and less than four weeks. HRP was the only one that kept stable when deposited over two weeks and less than four weeks. This method is effective, rapid, simple and low-cost and can be widely used for the stability characterization of polypeptides and glycoproteins.

Extraction of triazine herbicides from environmental water samples with magnetic graphene nanoparticles as the adsorbent followed by determination using gas chromatography-mass spectrometry

A novel method was developed for the determination of seven triazine herbicides in environmental water samples by magnetic solid-phase extraction with graphene-based magnetic nanoparticles (G-Fe3O4 MNPs) as the adsorbent coupled with gas chromatography-mass spectrometry detection. The main factors influencing the extraction efficiency including the amount of G-Fe3O4, the extraction time, the pH and the ionic strength of sample solution and the desorption conditions were optimized. Under the optimized experimental conditions, the enrichment factors of the method for the analytes were in the range from 574 to 968; the linearities of the method ranged from 0.01 to 10.0 microg/L for simazine, propazine, metribuzin, simetryn and cyanazine, from 0.05 to 10.0 microg/L for atrazine and from 0.01 to 8.0 microg/L for prometryn, with the correlation coefficients (r) ranging from 0.996 8 to 0.999 8. The limits of detection were in the range between 1.0 and 5.0 ng/L. Good reproducibilities were obtained with the relative standard deviations below 10.5%. The developed method was applied to the analysis of the triazine herbicides in different water samples (lake, well and tap). The recoveries of the method were in the range from 79.8% to 118.3% at the spiked levels of 0.5 microg/L and 2.0 microg/L. The results indicated that the developed method can be used as a simple and efficient technique for the determination of the triazine herbicides in environmental water samples.

Synthesis and characterization of sulfonic acid-functionalized SBA-15 for adsorption of biomolecules

Sulfonic acid-functionalized SBA-15 mesoporous molecular sieve (SBA-15-SO 3H and SBA-15-po-SO 3H) were synthesized by two post-synthesis methods. The functionalized SBA-15 materials were characterized and used to investigate the adsorption behavior for proteins (bovine serum albumin and cytochrome c) and polypeptides (insulin and glucagon) in the static and dynamic processes. The adsorption conditions: including adsorption time, pH and ionic strength of sample solution were investigated in the static adsorption mode. Under optimal conditions, SBA-15-SO 3H and SBA-15-po-SO 3H had a maximum capacity for insulin of 430 and 451 mg/g and for glucagon of 1303 and 1094 mg/g, respectively. In addition, the two functionalized SBA-15 mesoporous materials showed selectively adsorption of proteins in the dynamic adsorption mode. Compared with SBA-15, the surface modification of SBA-15 was responsible for the selective adsorption of proteins and high adsorption capacity for some biomolecules. It indicated that the two functionalized materials could be used as adsorbents to immobilize or separate biomolecules.