Ion-exchange Chromatography Research Articles

Effects of different extraction temperatures on the structural characteristics and antioxidant activity of polysaccharides from dandelion leaves.

Dandelion polysaccharides contribute to a variety of biological activities. This study evaluated the effect of different extraction temperatures (4°C and 80°C) on the structural characteristics and antioxidant activity of dandelion leaf polysaccharides (DLP). The findings demonstrated that the extraction efficiency improved at the higher temperature, while molecular weight exist a trend of degradation with increasing extraction temperature. Ion chromatography (IC) analysis indicated that the polysaccharides DLP4 and DLP80 were structurally complex heteropolysaccharides mainly composed of galactose, arabinose, glucose and mannose, with galactose and arabinose dominating. FT-IR and methylation analysis revealed that DLP4 and DLP80 had similar chemical structures and branches. DLP4 contained a higher amount of 6-Galactose. Microstructure analysis showed that heat treatment caused conformational changes in DLP4 and DLP80. Both had excellent free radical scavenging ability including DPPH·, ABTS·+, OH· and reducing power. The Reactive Oxygen Species assay indicated that the protective effect of DLP4 against H2O2-induced oxidative damage in vitro was stronger than that of DLP80. Superoxide dismutase (SOD) and malondialdehyde (MDA) measurements also confirmed that the antioxidant effect of DLP4 was more prominent. Overall, low temperature extracted DLP can be used as an antioxidant in the areas of food, medicine and biomaterials.

Introduction of curvature index and improvement of calibration curves used in ion chromatography (IC), atomic absorption spectrometry (AAS), UV-Vis spectrophotometry and catalytic combustion

Introduction of curvature index and improvement of calibration curves used in ion chromatography (IC), atomic absorption spectrometry (AAS), UV-Vis spectrophotometry and catalytic combustion

Concentrated solar energy-driven photothermal efficient degradation and mineralization of fluoroquinolone antibiotics in various water bodies

Sunlight-driven advanced oxidation process has promising applications and is increasingly receiving attention for the removal of emerging pollutants. This study describes a concentrated solar energy-driven photothermal (CSEP) system for the efficient degradation of fluoroquinolone antibiotics in wastewater. The operation of concentrating light degradation was not only a simple combination of thermal degradation and sunlight degradation, but the complex photothermal synergies that occurred to achieve degrading fluoroquinolones (FQs) more thoroughly. In the degradation processes, singlet oxygen (1O2), superoxide anion (O2·−), and hydroxyl radical (·OH) play pivotal roles. Additionally, the CSEP system exhibits outstanding defluorination capability (e.g., achieving 100 % defluorination for ciprofloxacin (CIP)). The photodegradation pathways of CIP were investigated using liquid chromatography/tandem mass spectrometry (LC/MS/MS), ion chromatography (IC) analysis, and density functional theory (DFT). Additionally, the aquatic ecological toxicity of CIP was assessed through ecological structure–activity relationship (ECOSAR) modeling and antimicrobial activity testing. Furthermore, this system demonstrates strong adaptability in addressing various natural water bodies, achieving an average degradation rate of over 98.00 % within a short time (3 min, 2 mg·L−1 CIP). In addition, it enhances the potential for synergistic removal of algae and degradation of FQs in complex water ecosystems. The adoption of this CSEP system holds considerable promise in furnishing robust technical support for the treatment and removal of FQs in water and wastewater.

Fluoride and nitrate contamination in groundwater of Naini Industrial Area, Uttar Pradesh: assessing non-carcinogenic human health risk

Groundwater is the main source of drinking water globally; however, its quality has been deteriorated due to various geogenic and anthropogenic activities. The groundwater quality of Naini Industrial Area, Prayagraj was studied seasonally to evaluate the fluoride and nitrate contamination pertaining to human health risk assessment. The samples were collected from 60 locations in the pre- monsoon, monsoon, and post-monsoon season. The fluoride and nitrate were assessed with the help of Ion chromatography. The NO3– concentration exceeded the Indian drinking water quality standards in 27% of the groundwater samples. The NO₃⁻ contamination is predominantly associated with agricultural practices, while F⁻ can be linked to natural geological sources. The non-carcinogenic human health risk assessment was quantified by calculating the Hazard Quotient (HQ) and Hazard Index (HI) were calculated as per USEPA methodology for male, female and child population. The findings indicate that the child population is particularly susceptible to health risks associated with the ingestion of F– and NO₃⁻ through the drinking water pathway. Across all the sampled sites, the Hazard Index (HI) values varied from 0.10 to 12.3 for males, 0.09 to 10.6 for females, and 0.16 to 19.7 for children suggesting substantial risk to the local populace at more than half of the locations which is largely related to nitrate contamination. Thus, the study suggests that groundwater at many locations is unsuitable for drinking without treatment pertaining to the probable health risk they pose to consumers advocating upgraded water management plan for the residents.

Scaling down recombinant carbonic anhydrase isolation with immobilized metal ion chromatography (IMAC): Harnessing enzymatic carbon dioxide capture and mineralization

BackgroundHuman activities have led to increased atmospheric CO2 levels, raising concerns about climate change. Carbonic anhydrase (CA) enzymes show promise for transforming CO2 into valuable products like calcium carbonate (CaCO3) through mineralization. Purifying and immobilizing CA enzymes on nanofiber membranes enhances their catalytic activity, enabling efficient CO2 conversion and mineralization. MethodsRecombinant CA was purified using immobilized metal affinity chromatography (IMAC), optimizing pH, biomass concentration, flow rate, and loading volume for maximum efficiency. The CA enzyme was then immobilized onto a weak ion exchange nanofiber membrane functionalized with AEA-COOH to create the CA-modified membrane (AEA-COOH-CA), enhancing CO2 conversion and CaCO3 mineralization. Significant findingsOptimal purification conditions (pH 7, 1 % biomass, 0.1 mL/min flow rate, 1.0 mL loading volume) were determined using IMAC. The CA-modified membrane effectively converted CO2 and mineralized CaCO3, demonstrating the potential for environmental CO2 sequestration. The immobilized CA activities of the AEA-COOH-CA nanofiber membranes exhibited 473.42 WAU/g-membrane, corresponding to 7.10 WAU per membrane piece. The CaCO3 precipitation reached 83.90 mg, with a precipitation efficiency of 11.82 mg CaCO3/WAU. These findings underscore the promise of enzymatic carbon capture using CA-modified membranes, offering a sustainable solution for greenhouse gas mitigation.

A Case Study on Ionic Compounds on the Aluminium 6061 Surface during Etching and Passivating Processes

The surface treatment process for aluminium is extensive, encompassing many methods and techniques. This study utilises chemical etching and passivation chemicals as surface treatment processes for chemical cleaning. The corrosion-controlled procedure involves the application of a powerful etchant solution to eliminate undesirable layers of material. Additionally, passivation chemicals are utilised as coatings to enhance the corrosion resistance of aluminium surfaces. Following the chemical cleaning process, ion chromatography testing was conducted to verify the ionic compounds on the surface of the aluminium metal subsequent to its reaction with etching and passivation chemicals. Different approaches were employed to address the ionic compound deposition problem on the surface of aluminium 6061 metal. Results revealed that replacing existing chemicals with new ones is the best solution to reduce the reading below the specified limit. However, one economically viable alternative for the chemical cleaning process is implementing an aeration process during rinsing, which offers greater economic efficiency compared to the substitution of chemicals entirely. Besides that, chemical cleaning via aeration procedure can mitigate the accumulation of ionic compounds on the material's surface.

Determination of Biogenic Amines in Foods by Capillary Electrophoresis

This review article intends to provide comprehensive analysis on biogenic amines, their source, type and classification. Biogenic amines are nitrogen containing organic compound that naturally exits in nature. They have low molecular weight and are basic in nature. They are active components that act as precursors for synthesis of alkaloids, hormones, protein, nucleotides and aromatic compounds. They occur in plants, animal and microorganism and play an essential role in metabolic and physiological function. This paper focuses on determination of biogenic amines in food by capillary electrophoresis as it causes several health problems such as histamine causes headache, digestive disorders and neurotoxicity, tyramine can cause hypertension and cadaverine can cause cytotoxixity. Biogenic amines are determined by several techniques such as high-performance liquid chromatography (HPLC), gas chromatography (GC), capillary electrophoresis (CE), Ion exchange chromatography. This paper further focuses on advantages of capillary electrophoresis in determination of biogenic amines in food. Capillary electrophoresis is a liquid phase separation method. The technique of capillary electrophoresis is used to identify, separate and quantify large range of components. There are several types of CE. Instrumentation of CE includes fused silica capillary which has length of 20 - 50cm. The reservoir is filled with separation buffer and the two ends of capillary tube extend between two reservoirs. CE has wide variety of application in identification, separation and quantification. In conclusion, biogenic amines cause many health hazard and is important to be identified and this paper deals with their presence and identifican in food.

Evaluation of Fluoride and Silver Ion Concentrations in e-Silver Diamine Fluoride and Advantage Arrest: An Ion Chromatography and Atomic Absorption Spectrometer Study

Evaluation of Fluoride and Silver Ion Concentrations in e-Silver Diamine Fluoride and Advantage Arrest: An Ion Chromatography and Atomic Absorption Spectrometer Study

Chemical composition of natural waters of the eastern rim of the Siberian platform (Predverkhoyansk foredeep)

Relevance. Foothill (foredeep, pericratonic) deflections play a significant role generation and localization of hydrocarbon accumulations and are promising objects of oil and gas exploration. Numerous studies of such structures are focused mainly on the geological structure, formation and prospects of oil and gas potential of these territories. Issues related to the geochemistry of natural waters are either not covered at all, or are presented in an extremely limited form. Studying the chemical composition of the natural waters of the Predverkhoyansk foredeep at the modern analytical level will make it possible to assess the hydrogeochemical background and emphasize the peculiarities of the behavior of the main components of the aquatic environment in conditions of a sharp change in the tectonic situation. Aim. To study and identify the hydrogeochemical features of the composition of natural waters in the area of the eastern framing of the Siberian platform in the region of its junction with the scaly-thrust dislocations of the Verkhoyano-Kolyma folded zone. Methods. Measurements of rapidly changing parameters of natural waters (pH, Eh, temperature) were carried out directly at the sampling site with a portable HQ-40D analyzer from Hach Lange (USA). The analysis of the chemical composition of waters was carried out in the Problematic Research Laboratory of Hydrogeochemistry of Tomsk Polytechnic University using ion chromatography methods on a two-channel reagentless ionochromatographic complex ICS-5000 with conductometric detection manufactured by Dionex-Thegmo Scientific (USA). The content of CO32– and HCO3–anions in the studied waters was determined by the traditional titrimetry method for these indicators. The Si concentration was determined by inductively coupled plasma mass spectrometry on a NeXION 300D device, Perkin Elmer (USA). Results. The paper presents the data on the chemical composition of the natural waters of the Tompo, Lyapiske, Sobolokh-Mayan (Sobopol) river basins in the inner part of the Predverkhoyansk foredeep in front of the cutting edge of the advanced thrust of the western slope of the Verkhoyansk anticlinorium. The patterns of the distribution of basic ions are shown, common features and differences between the studied sites are revealed.

In vitro analysis of a competitive inhibition model for T7 RNA polymerase biosensors

T7 RNA polymerase (T7 RNAP) biosensors, in which T7 RNAP transcribes some reporter gene or signal in response to external stimuli, have wide applications in synthetic biology and metabolic engineering. We adapted a biochemical reaction network model and used an in vitro transcription assay to determine network parameters for different T7 RNAP constructs. Under conditions where template DNA is limiting, the EC50 values of native and engineered T7 RNAPs ranged from 33 nM (29–37 95 % c.i.) to 570 nM (258–714 95 % c.i.) (wild-type T7 RNAP). The measured EC50 values were largely insensitive to free magnesium, pH, or other buffer conditions. Many biosensor configurations use a split RNAP construct, where the C-terminal (CT7) and N-terminal T7 (NT7) are fused to proximity induced dimerization modules. We used proteolysis and ion exchange chromatography to prepare a CT7 (80 kDa) product. The impact of free CT7 on T7 RNAP transcriptional activity was well described by a competitive inhibition model, with an inhibitory constant KI = 23 nM (18–28 95 % c.i.) of the sensor. These model parameters will be useful for forward modeling and design of T7 RNAP-based genetic circuits.

Construction of poly (styrene-divinylbenzene)@tris(4-aminophenyl)amine-p-phthalaldehyde-triethylenetetramine core-shell microspheres for the preparation of ion chromatography stationary phase

Core-shell composite microspheres are increasingly favored for the development of stationary phases due to their ability to integrate the monodispersity of inner core with the functional versatility of outer shell. In this study, poly(styrene-divinylbenzene)@(tris(4-aminophenyl)amine-p-phthalaldehyde-triethylenetetramine) (PS-DVB@TAPA-PPA-TETA) core-shell composite microspheres were constructed via an amine-aldehyde condensation reaction. The resultant microspheres were subsequently quaternized using the residual amine groups of TETA in the shell to create an effective anion-exchange stationary phase. The composite microspheres were characterized by SEM, FTIR, N2 adsorption-desorption experiment, et al. According to the results, the surface of PS-DVB microspheres was wholly covered by TAPA-PPA-TETA. The obtained PS-DVB@TAPA-PPA-TETA exhibited good reactivity, mechanical and chemical stability. The customized column exhibited good separation performance for seven conventional anions, five organic acids and three carbohydrates. The results demonstrate that PS-DVB@TAPA-PPA-TETA is a highly suitable material for the preparation of ion chromatographic stationary phase, exhibiting exceptional chemical stability and robust chromatographic performance in practical application. Finally, the customized column was successfully utilized for the determination of phosphate in waste acid sample.

Water-salt transport modeling and sulfate erosion mechanisms in cultural heritage under microclimate

The microclimate of the environment influences the characteristics of salt erosion in historical cultural heritage, determining the pattern of salt weathering and the mechanism of salt-action. The study focuses on the influence of cultural heritage microclimate on salt weathering, and the study aims to understand the weathering mechanism by combining the theory of crystalline weathering with temperature and humidity variation. The study comprised immersing sandstone from the Yungang Grottoes, a World Heritage Site, in a sulfate salt solution and simulation of salt deterioration under on-site cyclic variable temperature and humidity conditions. The article designs the accumulation of salt crystallization at the sandstone surface under the effect of salt evaporation and visualizes the water-salt migration and crystallization process by ion chromatography (IC), microelectrode, and hyperspectral techniques. The corresponding types of sulfate deterioration under different weathering simulation conditions are investigated to probe the mechanism behind different weathering patterns. In the first part, we construct a water-salt migration test, combining hyperspectral techniques and microelectrodes to visualize the weathering patterns, compositional characteristics, and spatial and temporal changes of water-salt transport based on Fick's law to establish a mathematical model and simulate it in COMSOL multi-physics field simulation software. Subsequently, tests were carried out on sandstone with sulfate solution at variable temperatures and humidity levels to simulate the process of salt weathering. The results demonstrated that different salt solutions, such as Na2SO4 and MgSO4, lead to the dissolution of clay minerals. At the same time, crystallization disrupts the sandstone structure, causing degradation of its physico-mechanical properties after several weathering cycles, and there are significant differences in the salt crystallization patterns at different temperatures and after cycling at different humidities.

Haemoglobin types and variant interference with HbA1c and its association with uncontrolled HbA1c in type 2 diabetes mellitus

Diabetes mellitus is among the leading global health concerns, causing over 1.5 million deaths alongside other significant comorbidities and complications. Conventional diagnosis involves estimating fasting, random blood glucose levels and glucose tolerance test. For monitoring purposes, long-term glycaemic control has been achieved through the measurement of glycated haemoglobin (HbA1c) which is considered reliable and preferred tool. However, its estimation could be affected by haemoglobin types like HbA0, HbA2, and HbF concentrations whose magnitude remains unclear as well as other haematological parameters. As such, the current study determined the association between HbA1c and haemoglobin types and determined correlation between haemoglobin types and haematological parameters among patients with type 2 diabetes mellitus (T2DM) compared to healthy non-diabetic participants. In this cross-sectional study, participants [n = 144 (72 per group), ages 23–80 years] were recruited and the desired parameter measured. HbA1c and other Haemoglobin variants were measured using ion-exchange high-performance liquid chromatography (HPLC) by the Bio-Rad D-10 machine (Bio-Rad Laboratories, Inc). Haematological parameters were measured using the Celtac G MEK-i machine (Nihon Kohden Europe). SPSS version 27 (IBM Corporation, Chicago, Illinois, United States) was used for the analysis. Chi-square (χ2) analysis, Mann-Whitney U test, Binary logistic regression and Pearson correlation were used to determine the differences between proportions, compare laboratory characteristics, associations and correlations respectively. With non-diabetics as the reference group, HbA1c was associated with increased HbA0 [OR = 1.509, 95% CI = 1.020–1.099, p = 0.003] and increased HbA2 [OR = 3.893, 95% CI = 2.161–7.014, p = 0.001]. However, there was no significant association between HbA1c and HbF [OR = 2.062, 95% CI = 0.873–4.875, p = 0.099]. Further, haematocrit (HCT) had a negative correlation with HbAO and a positive correlation with HbAS in participants with controlled diabetes. Mean cell volume (MCV) and mean cell haemoglobin (MCH) had a negative correlation with HbF. MCHC (mean cell haemoglobin concentration) had a negative correlation with HbA2 in participant with uncontrolled diabetes. The study concluded that levels of various haemoglobin types should be considered while monitoring glycaemic control through HbA1c. Additionally, MCHC should be considered in individuals with high concentration of HbA2 among T2DM patients while interpretating results for HbA1c.

Impact of Cooking Duration on Calcium Oxalate Needle-like Crystals in Plants: A Case Study of Vegetable Taro Flowers in Yunnan

As a popular vegetable in Yunnan Province, China, taro flowers are delicious but contain substances that can cause numbing and mucous membrane damage. Prolonged high-temperature cooking is used by locals to mitigate these effects, though its mechanisms were previously unexplored. This study confirms the presence of needle-like calcium oxalate crystals in taro flowers and shows that prolonged steaming reduces their quantity, size, and sharpness, making them safer to eat. Microscopic observations revealed numerous sharp-tipped (~50 μm) calcium oxalate crystals in fresh taro flowers. After 2 h of steam heating, there were significantly fewer (~80% reduction) and smaller crystals (~70% reduction). Ion chromatography showed no significant change (p > 0.05) in calcium oxalate content (remaining ~2.5% of dry weight) after heating. Higher temperatures increase calcium oxalate solubility, causing gradual dissolution and the likely formation of small irregular structures, thus reducing the numbing effect. Prolonged cooking could be applied to other plant-based foods and medicines rich in these crystals. By analyzing statistics related to taro and taro flowers, the estimated potential economic benefits of commercializing taro flowers were USD 2.58–12.92 billion annually, potentially improving food security, creating jobs, and promoting development across regions where taro is largely cultivated in the Global South.

Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5.

Microbial amylases should essentially remain active at higher temperatures, and in the alkaline pH and a range of surfactants to be suitable as detergent additives. In the present study, a thermophilic amylase producing bacterium, Bacillus licheniformis UDS-5 was isolated from Unai hot water spring in Gujarat, India. It was identified as a potent amylase producer during starch plate-based screening process. Therefore, the physicochemical parameters influencing amylase production were optimized using Plackett-Burman design and Central Composite Design. The amylase was purified through ammonium sulfate precipitation, size exclusion and ion exchange chromatography, achieving the purification fold and yield to be 9.2 and 40.6%, respectively. The enzyme displayed robust stability and activity across a wide range of temperatures and pHs, with an increased half-life and reduced deactivation rate constant. The amylase exhibited optimal catalysis at 70°C and pH 8. The kinetic studies revealed Km and Vmax values of 0.58mg/mL and 2528μmol/mL/min, respectively. Besides, the purified amylase displayed stability in the presence of various metal ions, surfactants, and chelators suggesting its potential for industrial applications, particularly in the detergent industry. Moreover, detergent application studies demonstrated its efficacy in enhancing washing performance. A comparative profile on washing efficiency of the studied amylase and the commercial amylase with various detergents pointed towards its possible future use as a detergent additive.

A biochemical mini-research project: Can yeast fermentation decrease the amount of phytic acid in soybeans?

This mini-research project studied the effect of yeast fermentation on the nutritional value of soybeans, specifically the concentration of phytic acid, protein, and lipids. With prior experience in protein and lipids analysis, the students (n = 12) in this laboratory applied their critical thinking and problem-solving skills to design and conduct their own assays for phytic acid extraction and quantification. All students decided to use ion exchange chromatography to extract phytic acid and UV/vis spectroscopy with Wade’s reagent for phytic acid quantification. These methods were evaluated by the students as simple and effective in analyzing phytic acid from soybeans. This project allowed students to use several of the methods used in other parts of the semester in addition to adding new methods to evaluate a research question.

Cost-effective low-volume sample introduction system for open tubular capillary ion chromatography via a hybrid negative pressure approach

Open tubular capillary ion chromatography (OTIC) exhibits a minute sample/eluent volume separation with a low-pressure operation (<30 psi) and potentially develops for a portable analytical device. In this study, a novel and cost-effective low-volume sample-introduction system has been developed for the OTIC and applied for separating water-soluble small inorganic ions. This innovative design uses a hybrid negative pressure (HNP) system, considerably reducing sample consumption to ∼7 μL per injection while maintaining excellent repeatability (<4 % relative standard deviation) on a 50 μm bore capillary tube. Various sample-introduction parameters, such as moving time of the sample zone, injection pressure, and sample viscosity, were optimized for the separation of model anions (Cl−, Br−, NO2−, and NO3−) and cations (Na+, K+, Ca2+, and Mg2+) in a non-suppressed OTIC mode. The performance of this HNP-OTIC system was demonstrated through the analysis of water-soluble small inorganic ion determination in low-volume particulate matter extracted samples, yielding recovery percentages of 78–119 % for cation determination in PM10 samples. In addition, the determination of K+ in PM2.5 samples exhibited no significant difference from the results obtained using a commercial packed IC. The cost-effective and efficient OTIC system has the potential to become a promising alternative for atmospheric analysis, especially in tracking K+ as an ion tracer for PM2.5 source identification.

Influence of Talc in Polypropylene on Total Fluorine Measurements Used as an Indicator of Per- and Polyfluoroalkyl Substances (PFAS).

Increasing restrictions for chemicals of concern in plastic packaging materials have created an urgent need to accurately detect and quantify these chemicals. Total fluorine measurements have been utilized to screen for highly scrutinized per and poly-fluorinated substances (PFAS) in food packaging materials. Inorganic contributions to the total fluorine signal can result in false positive signals exceeding regulatory limits. The purpose of this study is to develop a method for determining the contribution of talc inorganic filler to the total fluorine signal. The influence of talc on total fluorine measurements of plastics was evaluated by compounding talc with virgin polypropylene (PP) then measuring the total fluorine concentration using oxidative pyrohydrolytic combustion ion chromatography. This study provides a framework to predict the contribution of talc in plastic samples to the total fluorine signal. A near infrared spectroscopy method was developed by employing the full width half height (FWHH) of the interstitial fluorine characteristic band of talc. The FWHH signal of the processed puck specimens was determined to be linearly increase with the measured total fluorine difference as a function of talc concentration (R2 = 0.9619). This study developed a method to predict contribution of talc fillers to the total fluorine signal of plastic samples. This method is critical for accurately determining the regulatory compliance of talc filled plastic samples for PFAS using total fluorine. Total fluorine is a common regulatory compliance technique as an indicator of PFAS. Talc is a common plastic filler that contains fluorine as a contaminant. The fluorine in talc contributes to the total fluorine signal, which can falsely elevate the total fluorine signal, potentially resulting in the lack of regulatory compliance. The developed method serves as a framework of how to identify the fluorine contribution of inorganic fillers in plastics.

Optimization of fulvic acids production from oil palm empty fruit bunches using microwave extractor

Fulvic acid (FA) derives from a non-renewable source, Shilajit, known as highly commercial values for its benefit for human health. Fulvic acid can also be extracted from materials such as coal, lignite, and peat. Extraction methods of FA generally use solid acids and bases, ion exchange chromatography, and their combinations. However, these methods cause corrosion, low purity, and environmental pollution. The FA extraction using organic solvents is common, but low yielded, and many organic solvents are toxic. Therefore, an effective way to separate organic solvents from FA must be determined. This research aims to extract the FA from renewable biomass, namely oil palm empty fruit bunches (OPEFB), using a microwave extractor combined with hydrogen peroxide. The advantage of using a microwave is its quick and efficient extraction process. Hydrogen peroxide is an environmentally friendly solvent that can be converted into water and oxygen. Fulvic acid extraction was optimized using expert design with the Response Surface Methodology method with optimization of four 4 factors (H2O2 concentration and volume, reaction time, and microwave power). The extracted FA was then characterized using FTIR, H-NMR, and Fluorescennce spectroscopy. The highest FA concentration namely 24.716%, was obtained using H2O2 at a concentration of 30.46% with a volume of 137.4139 mL, reaction time of 9.384 minutes, and microwave power of 351.39 W. Fourier-Transform Infrared Spectroscopy peaks at 3213 cm-1, 2935.47 cm-1, and 2825.13 cm-1 in the OPEFB-FA sample indicate existence of FA. The fluorescent emission intensity ratio between 450/500 nm wavelengths of OPEFB-FA was 0.719.

TMET-12. TARGETING AMINO ACID METABOLIC VULNERABILITIES IN GLIOMAS

Abstract Isocitrate dehydrogenase (IDH) wild-type gliomas and IDH mutant gliomas are similar in histologic appearance but differ in their genetic and metabolic backgrounds. This study aimed to reveal the difference in metabolites between IDH-wildtype glioma and IDH-mutant glioma, and to find the effective treatment according to the status of IDH in gliomas. Two artificial cell lines made from human astrocyte were used: NHAE6E7hTERTRas (IDH-wildtype) and NHAE6E7hTERTIDHmut (IDH-mutant). Using capillary electrophoresis- and ion chromatography–coupled mass spectrometry, the amount of asparagine was lower in NHAE6E7hTERTRas cells compared with NHAE6E7hTERTIDHmut cells, whereas the amount of glutamine, glutamate and 2-oxoglutarate in NHAE6E7hTERTIDHmut cells was lower than NHAE6E7hTERTRas cells. L-asparaginase, which converts asparagine into aspartate, was more effective in NHAE6E7hTERTRas cells than NHAE6E7hTERTIDHmut cells. L-asparaginase induced autophagy and inhibition of autophagy by 3-MA suppressed L-asparaginase-induced antitumor effect, which meant that the antitumor effect was at least partially due to the L-asparaginase-induced autophagy. Pharmacological or genetical inhibition of GLUD1 which converts glutamate to 2-oxoglutarate, suppressed proliferation of the cells by inducing ROS and apoptosis in NHAE6E7hTERTIDHmut cells. ROS inhibitor, NAC suppressed GLUD1 inhibitor-induced ROS, apoptosis, and cytotoxicity in NHAE6E7hTERTIDHmut cells, revealing that cytotoxicity by GLUD1 inhibitor was at least partially due to the inhibitor-induced ROS. The experiments using mutant IDH1 overexpressed glioma cell line showed similar sensitivity to GLUD1 inhibitor to NHAE6E7hTERTIDHmut, which suggested that the difference of sensitivity to GLUD1 inhibitor was due to the status of mutant IDH. In vivo experiments, L-asparaginase suppressed the growth of xenografted glioma cells without mutant IDH, whereas GLUD1 inhibitor inhibited the proliferation of xenografted glioma cells with overexpressed mutant IDH1. In conclusion, L-asparaginase and Glud1 inhibitor will be new therapeutic option for IDH-wildtype glioma and IDH-mutant glioma, respectively.