Intertidal Mudflats Research Articles

Suboptimal Rootzone Growth Prevents Long Island (NY) Salt Marshes from Keeping Pace with Sea Level Rise

Salt marsh habitat loss and conversion are well documented across the marine-coastal district of New York. Regionally, these losses are characterized by marsh edge erosion, ditch and creek widening, internal ponding, and conversion from irregularly flooded marsh to regularly flooded marsh and intertidal mudflats. These changes in horizontal extent and shifts in vegetation composition suggest that NY’s salt marshes may not be keeping pace with sea level rise. To evaluate elevation building processes, deep rod surface elevation tables, marker horizons, and shallow rod surface elevation tables (SET-MHs and shallow RSETs) were installed as a network across Long Island, NY. Contributions of surface, shallow subsurface, and deeper processes to overall elevation changes were observed from 2008 to 2022. Using a linear mixed model approach, surface accretion, shallow subsurface rootzone growth, and deeper below-ground processes were evaluated against regional sea level rise, nutrient loading, and marsh area trends. We found that marshes on Long Island are not keeping pace with sea level rise because they lack vertical elevation growth within the rootzone. Optimizing conditions for belowground growth of native salt marsh plants and preservation of organic matter within the peat matrix is key for restoring salt marshes to a positive elevation trajectory relative to sea level rise. Much like a retirement savings account, knowing whether our marshes are increasing in elevation is important, but understanding the full suite of deposits and withdrawals is critical for managing this valuable resource for the future.

Individuals of a group-living shorebird show smaller home range overlap when food availability is low

BackgroundGroup living animals, such as shorebirds foraging on intertidal mudflats, may use social information about where to find hidden food items. However, flocking also increases intraspecific competition for resources, which may be exacerbated by food scarcity. Therefore, although aggregation may bring benefits, it may also increase the intensity of intraspecific competition.MethodsWe examined this trade-off in adult great knots Calidris tenuirostris, a molluscivorous long-distance migrating shorebird species, using interannual variation based on 2 years with different levels of food availability during their northward migratory staging in the northern Yellow Sea, China. We estimated individual home ranges and the extent of spatial overlap of home ranges of individually tagged birds in 2012 and 2015, whilst discounting for possible differences in body size, body mass, sex and migration schedule between years.ResultsWe found that home range size was not associated with body mass, arrival date, body size, or sex of the individual. Despite a significant difference in food availability between the two study years, there was no significant change in the 50% and 95% home range size of great knots in the contrasting situations. However, there was a significantly smaller spatial overlap between individuals in the year when food was less available, suggesting that great knots operated more independently when food was scarce than when it was abundant.ConclusionsThese results suggest that minimizing intraspecific competition became more important when food was scarce. Where it is impossible to monitor all habitats en route, monitoring the local movements of shorebirds may offer a way to detect changes in habitat quality in real time.

Shear and Scholte wave measurements on surficial intertidal mudflat sediments using medical sonoelastography techniques

In low-energy environments, the water–sediment interface often contains a dynamic fluffy transition layer (FTL) of suspended organic and inorganic matter, which is transformed by complex physical, biological, and chemical processes. The FTL mechanical properties are important for understanding carbon cycle dynamics and for robust sediment acoustic characterization, but erodibility and size make it difficult to study non-invasively and at sufficient resolution. In medical ultrasound sonoelastography, shear waves are excited within the body either using the acoustic radiation force (ARF) or via external vibration at the skin surface. Sub-micrometer displacements are measured remotely by subsequent ultrasound imaging at up to 10 kHz frame rate with approximately 0.5 μm spatial resolution. In this study, sediment cores were extracted from the intertidal mudflats from the Great Bay Estuary, USA. Compressional wave speed and attenuation measurements were performed using a core logger. Shear and Scholte waves were excited in the FTL using ARF- and external-vibration-based techniques and the motion detected with clinical ultrasound imaging arrays. Upward refraction of the shear wave was observed, and shear and Scholte wave speed and attenuation were measured. Finally, scanning electron microscopy was used to image the microstructure. Comparison between wave properties and microstructure will be discussed.

Mapping intertidal topographic changes in a highly turbid estuary using dense Sentinel-2 time series with deep learning

Intertidal mudflats are an important component of the coastal geomorphological system at the interface between ocean and land. Accurate and up-to-date mapping of intertidal topography at high spatial resolution, and tracking of its changes over time, are essential for coastal habitat protection, sustainable management and vulnerability analysis. Compared with ground-based or airborne terrain mapping, the satellite-based waterline method is more cost-effective for constructing large-scale intertidal topography. However, the accuracy of the waterline method is affected by the extraction of waterlines and the calibration of waterline height. The blurred boundary between turbid water and mudflats in the tide-dominated estuary brings enormous challenges in accurate waterline extraction, and the errors in estuarine water level simulations prevent the direct calibration of waterline heights. To address these issues, this paper developed a novel deep learning method using a parallel self-attention mechanism and boundary-focused hybrid loss to extract turbid estuarine waterlines accurately from dense Sentinel-2 time series. UAV photogrammetric surveys were employed to calibrate waterline heights rather than the simulated water levels, such that the error propagation is constrained effectively. Annual intertidal topographic maps of the Yangtze estuary in China were generated from 2020 to 2022 using the optimized waterline method. Experimental results demonstrate that the proposed deep learning method could achieve excellent performance in land and water segmentation in time-varying tidal environments, with better generalization capability compared with benchmark U-Net, U-Net++ and U-Net+++ models. The comparison between the generated topography and UAV photogrammetric observations resulted in an RMSE of 13 cm, indicating the effectiveness of the optimized waterline method in monitoring morphological changes in estuarine mudflats. The generated topographic maps successfully identified hotspots of mudflat erosion and deposition. Specifically, the mudflats connected to the land predominantly experienced deposition of 10–20 cm over the two-year period, whereas the offshore sandbars exhibited instability and significant erosion of 20–60 cm during the same period. These topographic maps serve as valuable datasets for providing scientific baseline information to support coastal management decisions.

A description of the genus Denitromonas nom. rev.: Denitromonas iodatirespirans sp. nov., a novel iodate-reducing bacterium, and two novel perchlorate-reducing bacteria, Denitromonas halophila and Denitromonas ohlonensis, isolated from San Francisco Bay intertidal mudflats.

The genus Denitromonas is currently a non-validated taxon that has been identified in several recent publications as members of microbial communities arising from marine environments. Very little is known about the biology of Denitromonas spp., and no pure cultures are presently found in any culture collections. The current epitaph of Denitromonas was given to the organism under the assumption that all members of this genus are denitrifying bacteria. This study performs phenotypic and genomic analyses on three new Denitromonas spp. isolated from tidal mudflats in the San Francisco Bay. We demonstrate that Denitromonas spp. are indeed all facultative denitrifying bacteria that utilize a variety of carbon sources such as acetate, lactate, and succinate. In addition, individual strains also use the esoteric electron acceptors perchlorate, chlorate, and iodate. Both 16S and Rps/Rpl phylogenetic analyses place Denitromonas spp. as a deep branching clade in the family Zoogloeaceae, separate from either Thauera spp., Azoarcus spp., or Aromatoleum spp. Genome sequencing reveals a G + C content ranging from 63.72% to 66.54%, and genome sizes range between 4.39 and 5.18 Mb. Genes for salt tolerance and denitrification are distinguishing features that separate Denitromonas spp. from the closely related Azoarcus and Aromatoleum genera. IMPORTANCE The genus Denitromonas is currently a non-validated taxon that has been identified in several recent publications as members of microbial communities arising from marine environments. Very little is known about the biology of Denitromonas spp., and no pure cultures are presently found in any culture collections. The current epitaph of Denitromonas was given to the organism under the assumption that all members of this genus are denitrifying bacteria. This study performs phenotypic and genomic analyses on three Denitromonas spp., Denitromonas iodatirespirans sp. nov.-a novel iodate-reducing bacterium-and two novel perchlorate-reducing bacteria, Denitromonas halophila and Denitromonas ohlonensis, isolated from San Francisco Bay intertidal mudflats.

Tandocyclinones A and B, Ether Bridged C-Glycosyl Benz[a]anthracenes from an Intertidal Zone Streptomyces sp.

Two new proton-deficient metabolites, tandocyclinones A and B (1 and 2), were discovered via the chemical profiling of the Streptomyces sp. strain TDH03, which was isolated from a marine sediment sample collected from the intertidal mudflat in Tando Port, the Republic of Korea. The structures of 1 and 2 were elucidated as new ether-bridged C-glycosyl benz[a]anthracenes by using a combination of spectroscopic analyses of ultraviolet (UV) and mass spectrometry (MS) data, along with nuclear magnetic resonance (NMR) spectra, which were acquired in tetrahydrofuran (THF)-d8 selected after an extensive search for a solvent, resulting in mostly observable exchangeable protons in the 1H NMR spectrum. Their configurations were successfully assigned by applying a J-based configuration analysis, rotating-frame Overhauser enhancement spectroscopy (ROESY) NMR correlations, chemical derivatization methods based on NMR (a modified version of Mosher's method) and circular dichroism (CD) (Snatzke's method using Mo2(OAc)4-induced CD), as well as quantum-mechanics-based computational methods, to calculate the electronic circular dichroism (ECD). Tandocyclinones A and B (1 and 2) were found to have weak antifungal activity against Trichophyton mentagrophytes IFM40996 with an MIC value of 128 μg/mL (244 and 265 μM for 1 and 2, respectively). A further biological evaluation revealed that tandocyclinone A (1) displayed inhibitory activity against Mycobacterium avium (MIC50 = 40.8 μM) and antiproliferative activity against SNU638 and HCT116 cancer cells, with IC50 values of 31.9 µM and 49.4 µM, respectively.

Habitat use across the tidal cycle by black-headed gulls breeding in the Wadden Sea

Movements of birds foraging in intertidal areas are often strongly linked to the tidal cycle, as water levels determine where and when birds can forage. The strength of this link likely depends on the ability to forage in habitats other than intertidal areas and on constraints imposed by breeding duties. Few studies have focused on the use of intertidal areas by generalists, such as the black-headed gull Chroicocephalus ridibundus, that occupy a wide variety of habitats within and beside intertidal mudflats. We investigated to what degree black-headed gulls (1) use intertidal mudflats versus terrestrial habitats during different phases of the tidal cycle, (2) follow the tidal wave to exploit recently exposed mudflats and (3) whether these behaviours are influenced by central place foraging. For this, 11 black-headed gulls breeding on the Wadden Sea island Griend were tracked during two years using GPS loggers. When commuting to and from Griend, up to 75% of their time was spent in intertidal areas during low and incoming tide, which increased to 92% when not behaving as central place foragers. While their movements were strongly linked to the tidal cycle, they did not follow the tidal wave across the tidal basin during either period. Rather, individuals foraged either predominantly west or east of Griend during low and incoming tide and mostly remained visiting these areas when not behaving as central place foragers. As one of the most abundant species in the Wadden Sea, the extensive use of intertidal mudflats highlights the importance of black-headed gulls within the intertidal food web of the Wadden Sea.

Estuarine benthic habitats provide an important ecosystem service regulating the nitrogen cycle

Globally, a key ecosystem service provided by sedimentary estuarine habitats is the regulation of nutrient cycles. The nitrogen (N) cycle is driven by complex biogeochemical transformations within these sediments—including nitrogen fixation, denitrification, assimilation and anaerobic ammonia oxidation—mediated by microorganisms. Evaluating ecosystem processes and their functional value is a knowledge gap for the wet-dry tropics and even more limited for macrotidal estuaries. The capacity of these important environments to withstand and assimilate increasing nitrogenous loads as a consequence of accelerating development pressures in tropical Australia is largely unknown. Because of the critical role nitrogen cycling plays in estuarine ecosystems, identifying important habitats that underpin N cycling, particularly denitrification known to mitigate anthropogenic N inputs, is important. Detailed benthic habitat mapping of the Darwin-Bynoe region of northern Australia has provided a rare opportunity to demarcate its key habitats, such as intertidal mudflats, seagrass, mangroves, reef and saltmarsh. Combined with new measurements of benthic nitrogen fluxes, it has been possible for the first time to map these processes and develop a simple integrated functional value for N cycling across key benthic habitats of a tropical macrotidal estuary. Maps generated in this process have provided broadscale identification of the functional importance of habitats with relevance to N removal processes. The role of intertidal sediments in denitrification has been highlighted. Furthermore, the study emphasises connectivity across benthic seascapes, where multiple services are likely to interact, in supporting overall function and ecosystem health. The distillation of composite processes in this mapping format allows resource managers and scientists to communicate outputs visually with a simple classification scheme which could be superimposed with additional data to support environmental assessment and management.

Genome assembly of the Korean intertidal mud-creeper Batillaria attramentaria

Batillaridae is a common gastropod family that occurs abundantly in the shallow coastal zone of the intertidal mudflats of the northwest Pacific Ocean, Australasia, and North America. In this family, Batillaria attramentaria is known for its biological invasion and colonization in estuarine and intertidal zones. It can endure and adapt the harsh intertidal conditions such as frequent temperature alteration, salinity, and air exposure. Therefore, we sequenced and assembled this Korean batillariid genome to get insight into its intertidal adaptive features. Approximately 53 Gb of DNA sequences were generated, and 863 scaffolds were assembled into a draft genome of 0.715 Gb with 97.1% BUSCO completeness value. A total of 40,596 genes were predicted. We estimated that B. attramentaria and Conus consors diverged about 230 million years ago (MYA) based on the phylogenetic analysis of closely related gastropod species. This genome study sets the footstep for genomics studies among native and introduced Batillaria populations and the Batillaridae family members.

CARBON STORAGE POTENTIAL OF DOMINANT MANGROVES IN WESTERN INDIAN SUNDARBANS

The Indian Sundarbans are noted for luxuriant mangrove diversity that is known to scrub carbon dioxide from the atmosphere. Precise estimation of the biomass of these species is necessary for evaluating the carbon storage pattern in the mangroves of the lower Gangetic belt. The plant biomass estimation was carried out for an average of 25 trees in 15 (10 m × 10 m) plots from the intertidal mudflats of Chemaguri (southeast portion of Sagar Island) in low tide conditions from 10th to 15th September 2022. The estimated biomass was of the order Sonneratia apetala > Avicennia alba > Avicennia marina > Excoecaria agallocha > Avicennia officinalis. The stem, branch, and leaf biomass of each species were converted into carbon by multiplying with a factor of 0.45 as per the standard procedure. The deviations observed in the results obtained from both studies call for the standardization of the process.

Unraveling antibiotic resistomes associated with bacterial and viral communities in intertidal mudflat aquaculture area

The extensive use of antibiotics in intertidal mudflat aquaculture area has substantially increased the dissemination risk of antibiotic resistance genes (ARGs). As hosts of ARGs, bacteria and virus exert vital effects on ARG dissemination. However, the insights for the interrelationships among ARGs, bacteria, and virus have not been thoroughly explored in intertidal mudflat. Therefore, this study attempts to unravel the occurrence, dissemination, evolution, and driving mechanisms of ARGs associated with bacterial and viral communities using metagenomic sequencing in a typical intertidal mudflat. Abundant and diverse ARGs (22 types and 437 subtypes) were identified and those of ARGs were higher in spring than in autumn. It is worthy noted that virus occupied a more essential position than bacteria for ARGs dissemination through network analysis. Meanwhile, nitrogen exerted indirect effect on ARG profiles by shaping viral and bacterial diversity. According to the results of neutral and null models, deterministic processes dominated the ARG community assembly by controlling sediment nitrogen and antibiotics. Homogeneous and variable selection dominated phylogenetic turnover of ARG community, contributing 46.15% and 45.90% of the total processes, respectively. This study can hence theoretically support for the ARG pollution control and management in intertidal mudflat aquaculture area.

Climatic effect from oyster reef restoration in Spartina alterniflora invaded intertidal mudflat: From the perspective of CH4 and N2O production

Spartina alterniflora is a dominant invasive species along the coast of China. A surprising phenomenon that the oyster reef formation is substituting the S. alterniflora invasion, was recently observed in the west coast of Bohai Bay, North China. As the intertidal mudflat ecosystem is critical for coastal blue carbon, this paper aims to evaluate the climatic effect of the alternation from the perspective of potential CH4 and N2O production. The sediment geochemical properties and potential CH4 and N2O productions were therefore measured in sediments dominated by Spartina alterniflora (SA), oyster reef (OR), mixture of S. alterniflora and oyster reef (SA-OR) and bare mudflat (BM) at an intertidal mudflat. Meanwhile, sediment microbial community structure and function genes were also determined utilizing metagenome high-throughput sequencing technology. Our results indicated that the formation of oyster reef in S. alterniflora invaded intertidal mudflat might slightly reduce the sediment potential CH4 production and enhance the sediment potential N2O production. The oyster reef formation could alter the community composition of methanogens and the abundance of functional genes in methanogenesis, and transform the main pathway from methylotrophic to hydrogenotrophic methanogenesis in surface sediments. Metagenome sequencing revealed that the oyster reef formation may promote nitrification, inhibit dissimilatory nitrate reduction (DNRA) and nitrate assimilation functions, and reduce the abundance of nosZ genes. Mantel analysis showed that pH, soluble salt and dissolved carbon (DC) were the most important factors influencing the composition of microbial community. In general, our results imply that the growth of oyster reef in the S. alterniflora invaded area has opposing effects from the prospective of the potential CH4 and N2O production.

Un-muddying the waters: Using benthic and shorebird population data to assess intertidal mudflat conditions and inform management strategies in two Irish Sea marine protected areas

Intertidal mudflats are highly productive coastal habitats, serving as a transition zone between terrestrial and marine ecosystems and providing a wealth of ecosystem services, including providing nursery and feeding grounds for many species of conservation and commercial importance. These habitats are important for wintering shorebirds and waders, which depend on them for food, nesting, and migration. Their importance was formally recognised in 1971 following the Ramsar Convention on Wetlands of International Importance, yet their conservation status remains largely uncertain as they are difficult to survey, and a complete understanding of their complex biophysical processes has yet to be established. While intertidal mudflats are found all around the UK coastline, there are few routine field surveys of these habitats, making effective conservation in the face of rising anthropogenic and climate pressures challenging. Marine protected areas (MPAs) across the UK list mudflats and sandflats not covered by seawater at low tide as a designation feature separate from saltmarshes, but they are often managed together – where management exists. A lack of resources has resulted in several paper parks around the UK, where MPAs are designated but not adequately monitored or managed. This study explores the use of population data of benthic species and shorebirds as a proxy for temporal changes in mudflat conditions, focusing on two MPAs in the Irish Sea, Strangford Lough in Northern Ireland, and the Solway Firth in Scotland/England. It finds that the two methods together can provide information useful to support MPA management, but this approach is hampered by poor availability of sufficient data. Such data limitation can impact what it means to achieve or maintain favourable conditions for these habitats.

Studies on modulation of hemocyte surface antigen through agglutination reaction under arsenic toxicity in edible mudcrab (Scylla serrata)

Scylla serrata (Crustacea: Decapoda), which is widely spread on the intertidal mudflat of West Bengal, India's Sundarbans Biosphere Reserves, is a potential aqua crop and an economically significant edible species. One of the larger crab groups in the mangrove swamp of the Sundarbans is thought to be this one. The S. serrata's multifaceted immune response is directly tied to its diverse habitat and survival technique. It lives in dangerous surroundings and is constantly in danger of physiological stress brought on by various xenobiotics, such as arsenic. By producing a number of polyclonal antisera in rabbits (New Zealand White, albino), the study attempted to evaluate the surface antigen against crab hemocytes and murine lymphocytes. Control hemocytes and hemocytes treated to 1 ppm expressed very identical reactivity to antihemocyte sera for the agglutination reaction. The control results, however, shifted when exposed to 2 and 3 ppm of sodium arsenite, indicating arsenic-induced hemocyte surface modification. The agglutination reaction from the control sets of hemocytes that reacted with murine anti-lymphocyte sera gradually, shifted as the quantity of sodium arsenite in the medium of the treatment sets increased. The maximum equivalence zone of murine lymphocyte and hemocyte agglutination 98.6% and 99% respectively suggested a potential epitope sharing between two phylogenetically separate species. The situation may lead to a possible alteration of immune status and make opportunity for pathogenic foreign invaders within the mud crab body. Chronic arsenic exposure indicated a steady decline of edible and demandable S. serrata in the natural habitat of Sundarbans.

The exploitation of biofilm by migrant western sandpipers (Calidrismauri)

Assessing the quality of migratory shorebird stopover sites requires good measures of food availability. We developed simple methods to measure biofilm grazing by migrant western sandpipers (Calidris mauri), a species for which biofilm is an important dietary component. We used a field-portable chlorofluorometer to measure the density of chlorophyll-a (Chl-a) in surficial biofilms on Roberts Bank, a large intertidal mudflat in British Columbia, Canada, during northward migration.Chl-a density begins at a low level during each diurnal emersion period, and increases steadily during emersion at 4.1 mg m−2 h−1 for a total of ∼24.6 mg m−2 over a typical 6 h emersion period and ∼41 mg m−2 over a 10 h emersion period. Western sandpipers grazed at 1.35–1.45 mg Chl-a m−2 min−1, thus biofilm production supports 17.6 min m−2 of grazing time during a 6 h low tide period and 29.3 min m−2 during a 10 h period. During peak northward migration, the average grazing intensity of western sandpipers over an intertidal emersion period was 3.3–6.4 min m−2, suggesting that biofilm accumulation was 2.7–8.8 fold greater than the amount consumed. We found Chl-a density was highest (∼65 mg per m2) within 40 m of the shoreline. Grazing intensity was lowest close to shore, where predation risk from falcon attacks is highest. Grazing intensity peaked at 240 m and then declined, lowering Chl-a density at greater distances to a uniform level of ∼54 mg m−2. These results indicate that interactions between biofilm production and sandpiper grazing underlie spatio-temporal patterns in biofilm abundance on Roberts Bank.

Genome-wide identification and expression analyses of Toll-like receptors provide new insights on adaptation to intertidal benthic environments in Urechis unicinctus (Annelida, Echiura)

Toll-like receptors (TLR) are an important class of pattern recognition receptors involved in innate immunity that recognize pathogen-associated and damage-associated molecular patterns. Although the role of TLRs in immunity has been extensively studied, a systematic investigation of their function in environmental adaptation is still in its infancy. In this study, a genome-wide search was conducted to systematically investigate TLR family members of Urechis unicinctus, a typical benthic organism in intertidal mudflats. A total of 28 TLR genes were identified in the U. unicinctus genome, and their fundamental physiological and biochemical properties were characterized. Gene copy number analysis among species in different habitats indicated that TLR family gene expansion may be probably related with benthic environmental adaptation. To further investigate the expression patterns of TLR members under environmental stress, transcriptome data was analyzed from different developmental stages and the hindgut under sulfide stress. Transcriptome analysis of different developmental stages showed that most TLR genes were highly expressed during a key period of benthic environment adaptation (worm-shaped larva). Transcriptome analysis of the hindgut under sulfide stress showed that the expression of 12 TLR members was significantly induced under sulfide stress. These results indicate that the regulation of TLR gene expression may be probably involved in the adaptation of U. unicinctus to the benthic intertidal zone environment. Taken together, this study may lay the foundation for future functional analysis of the specific role of TLRs in host immune responses against sulfide exposure and benthic environmental stress in annelid.

Geomorphological Changes by the Action of Coastal Processes and Anthropogenic Activties along the Coastal Zone between Ras Al-Jalaiah And Ras Az-Zour, Southern Kuwait

The coastline is one of the most important linear features on the earth’s surface, which displays a dynamic nature. The natural processes that interact with the coastal environment of Kuwait are tides, currents, and waves. The main shoreline of the coast of Kuwait is about 325 km long. In the north, the coast is characterized by wide intertidal mudflats, bounded by a large-scale coastal sabkha, partly covered with sand drifts. The southern shore is characterized by relatively steep sand beaches with narrow to moderately wide rocky intertidal platforms, which are partly covered by sand and algal mats. The area of interest is the coastal area between Ras al-Julaiha and Ras Az-Zour in the southern area of Kuwait. Sandy berm and wave-cut cliff bound this southern intertidal environment. The study area is a superficial, open, vulnerable bay of a maximum depth of 28 m extending along the southern shore of Kuwait. Ras Al-Jalaiah promontory bounds the area northwards and Ras Az-Zour promontory bounds it southward, with a distance measured vertically of 18.085 km.

UAV-acquired imagery with photogrammetry provides accurate measures of mudflat elevation gradients and microtopography for investigating microphytobenthos patterning

Intertidal mudflats are highly productive ecosystems where elevation gradients and complex microtopography drive the growth of benthic microalgae (microphytobenthos; MPB) that form the basis of estuarine foodwebs and are crucial for nutrient cycling, shoreline stabilization, and the persistence of marine and coastal species. Mudflat ecosystems are threatened by human activity and natural stressors and thus need to be mapped and monitored. Unoccupied aerial vehicle (UAV) technologies and digital aerial photogrammetry (DAP) have been successfully implemented to study mudflat environments. However, standardizing the UAV flight parameters needed for optimal DAP performance on mudflats remains outstanding. Here, we systematically determined the optimal flight parameters for collection and photogrammetric processing of UAV-acquired data on mudflats by (1) testing across-track overlap (50, 70, and 90%) and flight elevation (73 m and 110 m) parameters, assessing the accuracy of DAP results against reference data from a mobile laser scanner (MLS), and (2) comparing semi-variograms of digital surface models (DSMs) from two UAV flight elevations. We found that all combinations of UAV flight parameters yielded accurate DAP products; flight elevation had a marginal effect on image alignment and had no effect on accuracy, while across-track overlap had no effect on image alignment of DSM of difference (DoD) values. All UAV and MLS point clouds were aligned with and accuracy of < 0.016 m and absolute values of mean DoDs were all sub-millimeter, ranging from 0.0001 ± 0.0322 to 0.0083 ± 0.0270 m. We conclude that conducting UAV surveys at 110 m elevation with 50% across-track image overlap is sufficient for high-accuracy DAP in mudflats. Finally, we tested the utility of such fine-scale topographic data for ecological applications by comparing elevation and topographic position indices (TPI) of DAP-derived DSMs to MPB abundance, measured as chlorophyll a (chl-a), calculated from UAV-acquired NDVI data. We found that elevation and TPI account for 1.6–17% of the variation in chl-a concentration, and that these relationships depend on distance from shore and mudflat morphology. Our findings contribute to standardizing the application of UAV technologies in mudflats and demonstrate the potential of UAV-acquired data for modeling the relationship between microtopography and MPB on ecologically important mudflats.

UAV Photogrammetry in Intertidal Mudflats: Accuracy, Efficiency, and Potential for Integration with Satellite Imagery

The rapid, up-to-date, cost-effective acquisition and tracking of intertidal topography are the fundamental basis for timely, high-priority protection and restoration of the intertidal zone. The low cost, ease of use, and flexible UAV-based photogrammetry have revolutionized the monitoring of intertidal zones. However, the capability of the RTK-assisted UAV photogrammetry without ground control points, the impact of flight configuration difference, the presence of surface water in low-lying intertidal areas on the photogrammetric accuracy, and the potential of UAV/satellite Synergy remain unknown. In this paper, we used an RTK-assisted UAV to assess the impact of the above-mentioned considerations quantitatively on photogrammetric results in the context of annual monitoring of the Chongming Dongtan Nature Reserve, China based on an optimal flight combination. The results suggested that (1) RTK-assisted UAVs can obtain high-accuracy topographic data with a vertical RMSE of 3.1 cm, without the need for ground control points. (2) The effect of flight altitude on topographic accuracy was most significant and also nonlinear. (3) The elevation obtained by UAV photogrammetry was overestimated by approximately 2.4 cm in the low-lying water-bearing regions. (4) The integration of UAV and satellite observations can increase the accuracy of satellite-based waterline methods by 51%. These quantitative results not only provide scientific insights and guidelines for the balance between accuracy and efficiency in utilizing UAV-based intertidal monitoring, but also demonstrate the great potential of combined UAV and satellite observations in identifying coastal erosion hotspots. This establishes high-priority protection mechanisms and promotes coastal restoration.

Erosion and accretion patterns on intertidal mudflats of the Yangtze River Estuary in response to storm conditions

Understanding of erosion and accretion patterns over intertidal mudflats during storm periods is vital for the management and sustainable development of coastal areas. This study aimed to investigate the effect of the 2014 storm Fung-wong on the erosion and accretion patterns of the Nanhui intertidal mudflats in the Yangtze estuary, China, based on field measurements and Delft3D numerical modeling. Results show that prolonged easterly winds during the storm enhance the flood velocity, weaken the ebb velocity, and even change the current direction. The current velocity, wave heights, and bed-level changes increased by 1–1.43 times, 2.40–3.88 times, and 2.28–2.70 times than those of normal weather, respectively. The mudflats show a spatial pattern of overall erosion but increasing erosion magnitude from the high (landward) mudflat to the low (seaward) mudflat during the storm. The magnitude of bed-level change increases with increasing wind speed, but the spatial pattern of erosion and accretion remains the same. The main reason for this pattern is the longer submersion duration of the low mudflat compared with the high mudflat, so the hydrodynamic process is longer and stronger, leading to an enhancement in bed shear stress and sediment transport rate. Wind speed increases the hydrodynamic intensity but does not affect on the submersion duration over each part of the intertidal mudflat. This study is helpful to improve the understanding of physical processes during storms on intertidal mudflats and provides a reference for their protection, utilization, and management, as well as for research in related disciplines.