Presence and agency in lifestyle medicine: A neuropsychobiological framework for sustainable health transformation

Synthetic varroacides in honey bee colonies: A comprehensive monitoring program across the European Union

Current advances in restoring intestinal barrier homeostasis by natural medicines.

The dinuclear CuII-carboxylate paddlewheel, a linchpin of metal-organic cages (MOCs), is highly labile in solution. Herein, more kinetically stable heterometallic (NiPd)2L4 lantern-type helical cages have been assembled from preformed [NiPd(OAc)4] precursors. Ligand exchange processes (monitored by HPLC) revealed the half-lives of the (NiPd)2L4 cages in solution to be 103 times greater than those of the Cu4L4 analogues. The hydrophobic guest p-chloranil was encapsulated and reacted inside the unique noncoordinating, charge-neutral PdII···PdII cavity of a (NiPd)2L4 cage but not in the analogous Cu4L4 cage. The NiII-PdII paddlewheel system provides a kinetically stable yet synthetically accessible alternative to labile CuII-based metallosupramolecular cages that are more long-lived in solution and have unique internal environments.

Endothelial-to-Mesenchymal Transition (EndMT) is a regulatory mechanism integral to the normal developmental processes of the organism, playing a pivotal role in sustaining the homeostasis of the intravascular milieu. However, when the internal environment exceeds the endothelium's compensatory capacity, excessive EndMT can precipitate pathological alterations. EndMT is particularly significant in the pathogenesis of pulmonary diseases, including pulmonary fibrosis, pulmonary hypertension, and lung cancer. The regulatory mechanisms underlying EndMT are intricate and multifaceted. Numerous signaling pathways have been implicated in EndMT modulation, with the TGF-β pathway emerging as a central inducer. Furthermore, research has identified that epigenetic modifications, metabolic processes, hypoxia, inflammatory cytokines, and oxidative stress all contribute to the regulation of EndMT. This complexity renders the interplay between EndMT and pulmonary vascular diseases exceedingly intricate. In this manuscript, we provide a comprehensive overview of the signaling pathways and other influencing factors that govern EndMT. Concurrently, we meticulously delineate potential therapeutic targets to offer novel avenues and insights for the management of pulmonary diseases. Our synthesis of the current understanding of EndMT regulation is intended to inform future research and clinical strategies, potentially leading to more effective interventions for patients afflicted with pulmonary disorders.

Hypoxia-driven dysregulated lactate metabolism is a feature of intervertebral disc degeneration (IDD). However, biological metabzymes and most biomimetic counterparts are ineffective under hypoxic conditions and often generate harmful metabolites. Here, a single-atom nanozyme is presented that exhibits catalytic activity rivaling a biological metabzyme and pathological context-aware metabolism functions within the male rats' disc microenvironment, termed the "biogenic Fe-N-C artificial metabzyme". The spatially dynamic metabolomics and assessments of IDD tissues demonstrate that biogenic Fe-N-C artificial metabzyme efficiently catalyzes the conversion of excess lactate into pyruvate and reduced glutathione within IDD. Subsequent metabolic convergence of mitochondria and extracellular matrix coordinates the recovery of the internal and external nucleus pulposus cells environment, constituting IDD metabolic therapy. The current work proves that creating artificial metabzymes tailored to the pathological tissue environment can be used to correct metabolic dysfunction.

Purpose of research of this article is to analyze the problem of maintaining a microclimate in religious buildings that is optimal for preserving church buildings and their interior decorative elements (wall paintings, iconostases, icons, etc.), and to identify ways to improve the energy efficiency of ventilation and air conditioning systems. Methods. A study of the moisture content of the enclosing structures of the Resurrection Church revealed significant excess of regulatory values in the basement and on the first floor, confirming the presence of persistent moisture zones that affect the durability of the masonry and decorative coatings. Scanning microscopy analysis of plaster samples revealed structural heterogeneity and varying degrees of degradation caused by localized fluctuations in humidity, temperature fluctuations, and exposure to soot. Results. The spatial distribution of damage allowed us to establish a relationship between the condition of the engineering systems and the deterioration of the decorative layer. Archaeological work in 2015 contributed to improved drainage conditions and partial stabilization of the humidity regime. Based on the data obtained, key risk areas were identified and recommendations for upgrading utility systems were developed. Particular attention was paid to the need to implement energy-efficient ventilation and air conditioning with heat recovery. Conclusions. The analysis showed that the existing microclimate of the Resurrection Church does not ensure the preservation of the structures and decorative elements, and the identified areas of high humidity create conditions for accelerated deterioration of materials. To stabilize the internal environment, a comprehensive modernization of utility systems is required, including the installation of energy-efficient supply and exhaust ventilation with heat recovery, proper air exchange, and the use of modern air conditioning systems. Compliance with the requirements of Code of Practice 60.13330.2020, State Standard 30494-2011, and the provisions of Federal Law No. 261 will reduce heat loss, lower operating costs, and ensure stable temperature and humidity conditions. Implementation of the proposed measures will create conditions for the long-term preservation of the architectural structure and its artistic content.

Cellular crosstalk in doxorubicin-induced cardiotoxicity: underlying mechanisms and therapeutic strategies.

De novo synthesis of infectious bacteriophage MS2 labelled with dyes: Investigation of the electrical properties of its interior.

Optimizing landing mechanics to modulate patellar tendon loading: An individualized moment arm analysis.

An AI-enabled micro-level analysis of organizational and technological paths to green upgrading

Intracellular Zn²⁺ dynamics regulate cefiderocol resistance in Klebsiella pneumoniae.

The OSCA family proteins are pivotal mechanosensitive ion channels in plants, playing a crucial role in sensing osmotic stress and initiating drought adaptation pathways. Despite significant progress in structural biology, the dynamic conformational pathway of gating, the cooperative mechanism of key residues, and the mechanism of selective permeation for different cations remain largely elusive. In this study, we employed molecular dynamics (MD) simulations to systematically investigate the Arabidopsis OSCA3.1 channel in its contracted, expanded, and mutant open states. Conventional MD simulations reveal that the transition from the contracted to the open state is accompanied by a significant conformational rearrangement involving the tilting of the M6 helix. This movement drives the radial displacement and reorientation of key hydrophobic residues (e.g., F504, F505), thereby dismantling the hydrophobic gate composed of continuous hydrophobic residues and creating physical space for ion permeation. Steered molecular dynamics (SMD) simulations show that both K+ and Ca2+ must overcome substantial resistance when traversing the hydrophobic constriction. However, due to its higher charge density and dehydration energy, Ca2+ requires a significantly larger driving force than K+. Umbrella sampling calculations yielded potential of mean force (PMF) profiles, quantitatively revealing the free energy barrier differences for the permeation of the two ions for the first time. The results indicate that although their permeation pathways are similar, Ca2+ faces a significantly higher free energy barrier than K+ (Ca2+: ∼53 kcal/mol vs K+: ∼30 kcal/mol) due to stronger hydration and the higher energy cost of dehydration associated with its higher charge density. Electrostatic potential analysis further shows that the hydrophobic gating region within the pore is electrically neutral, facilitating the passage of various cations, while the lower part is electronegative, a distribution that may favor initial ion entry and subsequent guidance. This study demonstrates that the opening of the OSCA3.1 channel is a highly cooperative conformational reorganization process dominated by helical motion. Although its internal environment allows for the passage of multiple cations, from an energetic perspective, the conduction of monovalent K+ is more favorable in this expanded state. These findings demonstrate that OSCA proteins exhibit a "kinetic selectivity" rather than being "completely nonselective," where mechanical force or osmotic pressure modulates the degree of channel opening, thereby dynamically affecting the selective transport efficiency of potassium and calcium ions. This provides a novel atomistic mechanism and energetic theoretical basis for understanding how plants differentially regulate the transmembrane flux of the osmotic balance ion (K+) and the signaling ion (Ca2+) through the same channel when sensing osmotic stress.

Key challenges and novel encapsulation technologies for probiotics stability improvement: A review.

Subject. This article discusses the impact of changing conditions in the external and internal environment on the financial and economic performance of PAO Inter RAO in 2020–2024. Objectives. The study aims to analyze the features of the operation of Russia's largest energy company, PAO Inter RAO, in the context of economic transformation. Methods. For the study, we used the methods of horizontal and vertical analyses, comparative analysis, and ratio analysis of the company's indicators. Results. The article reveals that despite the introduction of extensive economic restrictions since 2022, PAO Inter RAO demonstrates a high degree of resilience. Conclusions. The combination of changes in revenue structure, investment and dividend policy, as well as liquidity management, forms a sustainable adaptive trajectory under conditions of external shocks and increasing geoeconomic pressure.

Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis (TB), survives and replicates within macrophages, key immune cells that normally eliminate pathogens. How macrophages control their internal cellular environment in response to infection remains incompletely understood. One such important cellular control system is ubiquitylation, in which proteins are tagged with ubiquitin to determine their functional fate or target them for degradation. We recently identified the GID/CTLH E3 ligase ubiquitylation complex as a critical modulator of macrophage antimicrobial responses to Mtb. Here, we used proteomics approaches to define the proteins controlled by the GID/CTLH complex in Mtb-infected macrophages. We found that this complex ubiquitylates a broad network of proteins involved in cellular metabolism and immune signaling. When the complex is disrupted, macrophages undergo extensive metabolic reprogramming, particularly increased mitochondrial energy production, while showing reduced inflammatory signaling. Despite this dampened immune response, these cells are better able to restrict Mtb growth. We also identified the phosphatases PTEN and INPP5D as targets controlled by the GID/CTLH complex that independently influence intracellular bacterial survival. Our findings demonstrate that the GID/CTLH complex is a critical regulator of metabolism and immune function, shaping the outcomes of Mtb infection.

In this paper, we introduce a class of stochastic Hopfield neural network with cross-diffusion (SHNNCD), and for the first time, investigate the existence of the ergodic stationary distribution for SHNNCD under time-varying modified Markov switching. In terms of the internal structure, we incorporate cross-diffusion to fully account for the coupling effects between different neuronal states. In terms of the external environment, compared with traditional Markov switching, we consider a class of Markov switching modified by time-varying functions, including periodic, decaying, and random types. Based on these innovations in both internal structure and external environment, we construct a global Lyapunov function for SHNNCD using graph theory under the consideration of cross-diffusion, and derive the criterion for the existence of the ergodic stationary distribution of SHNNCD. Finally, we verify the validity of our theoretical results through numerical simulations.

Purpose The purpose of this study is to build upon the research, Identifying Stress Reduction Factors in Built Environment Through Medical Staff Involvement (Waroonkun et al., 2024), by summarizing design improvements for outpatient ward working areas aimed at reducing medical staff stress. Through staff involvement and qualitative analysis of interview data, this research synthesized key concepts into an effective design guideline model. To ensure the consistency and practical implementation of these design data, the new model was subsequently evaluated by the same cohort of medical staff from the initial study. Design/methodology/approach This study comprised three stages: Designing the internal environment of medicine and paediatric out-patient wards, and incorporating the stress reduction factors identified in the preceding study. Conducting in-depth interviews with the original group of medical staff by using 3D renderings of the proposed designs, to ascertain their suitability for stress reduction. Developing design guidelines to serve as a model for future out-patient ward designs. Findings Three main themes were identified from the interviews analysis: Theme 1: Factors affecting efficiency at work, which includes accessibility, lighting/visibility; Theme 2: Factors affecting stress levels, which includes accessibility, optimizing spatial delineation and directional signage for effective navigation; and Theme 3: Factors conducive to relaxation, including relaxation area and recreation space in the paediatric ward. Originality/value This study builds upon previous research that compared medicine and paediatric out-patient ward designs at a public hospital, namely, Maharaj Nakorn Chiang Mai Hospital in Chiang Mai, Thailand. Its focus is on environmental improvements, specifically the physical environment factors that can reduce stress among medical staff. The designs, based on the previous study's findings, were refined through surveys with medical staff, to ensure they met their needs, while adhering to hospital design standards. This research is novel and unprecedented, because a continuation study focused on improving the physical environment within a Thai public hospital has never been done before.

The building façade serves as the primary interface between the built environment and external climate, marking the transition from static regulation to dynamic response in climate-adaptive design. While existing research predominantly addresses periodic climatic elements such as temperature and solar radiation, the highly stochastic wind environment and its potential for internal acoustic problems remain systematically unexplored. This study investigates the acoustic modulation mechanism of building façades under dynamic wind conditions through a simulation-based methodology. The primary aim is to demonstrate the use of active control to mitigate the influence of fluctuating wind on the internal acoustic environment of buildings with open windows or semi-open boundaries, focusing on the coupling between stochastic wind fields and architectural acoustics in humid subtropical climates. We propose a wind-responsive adaptive acoustic façade system employing fractal geometry and configurable delay strategies, and develop a high-fidelity simulation framework to quantify how façade geometry and activation logic regulate acoustic parameters under varying wind conditions (1–8 m/s). Results indicate that: (1) support vector regression-based mapping of wind speed to delay strategies maintains key sound-field parameters (Lateral Fraction (LF), Speech Clarity (C50), and Early Decay Time to Reverberation Time ratio (EDT/RT30)) within 10% fluctuation across wind regimes; (2) fractal configurations achieve balanced wide-band (125 Hz–8 kHz) performance, with SPL fluctuation <3 dB, spectral tilt (+0.3 dB), and reverberation time slope <0.3; (3) configurational switching between column (high LF) and row (high C50) arrangements enables dynamic trade-off between spatial impression and speech clarity. This work establishes an integrated framework coupling wind dynamics, façade morphology, and acoustic modulation to regulate objective indoor acoustic parameters. Based on the simulated omnidirectional point-source model, the results show that key acoustic indicators remain stable across varying wind conditions, providing a theoretical and quantifiable basis for climate-responsive acoustic envelope design. Future work will include empirical prototype testing and listening tests to determine whether these simulated acoustic parameters translate into improved comfort and well-being for occupants.

The purpose of the research is to explore the possibilities of structuring the process of developing a business strategy and analyzing the conceptual framework of managers involved in its formation. The methodological basis of the research. The research is based on an empirical information base, which was formed using expert interviews of specialists in the field of strategy development and a questionnaire survey of the management staff of commercial companies. Methods of comparative, structural, logical, and semantic analyses were used to process the information received. Methods of tabular and graphical representation of information, construction of conceptual models are used to present the results. The results of the study. As part of the research, a «reference» (theoretically sound) approach to creating a business strategy was developed, which served as the basis for further analysis of practical experience. A conceptual model of the relevant process is proposed as a tool for structuring management actions in the formation of a business strategy. It is based on a consistent analysis of the most important factors of the external and internal environment, which makes it possible to systematize the key stages of strategic planning. The elements of the «reference» conceptual framework of managers involved in the process of developing a business strategy are identified. To analyze the relationships between these elements and the components of the «reference» process, a matrix tool has been developed that allows you to evaluate these relationships. Its application makes it possible to identify the elements of the conceptual apparatus, practical managers, which have the most significant impact on the quality of the results of the business strategy development process. Based on the matrix analysis, «gaps» in the structure of the conceptual apparatus of practical managers are identified, which have the most significant impact on the quality of the results of the business strategy development process implemented in practice. The prospects of the research are related to the further study of the interrelationships of the conceptual apparatus of managers with the specifics of the implementation of various aspects of their activities, as well as the analysis of the adjustment of the structure of the conceptual apparatus in improving the effectiveness of corporate training programs implemented in companies.