The influence of the isospin-independent and isospin-dependent equation of state (EoS) with momentum dependence, as well as the Coulomb interaction on the pion production in intermediate energy heavy-ion collisions (HICs), is studied for both isospin-symmetric and neutron-rich systems. The Coulomb interaction plays an important role in the reaction dynamics, and strongly influences the rapidity and transverse momentum distributions of charged pions. It even leads to the π−/π+ ratio deviating slightly from unity for isospin-symmetric systems. The Coulomb interaction between mesons and baryons is also crucial for reproducing the proper pion flow since it changes the behaviour of the directed and the elliptic flow components of pions visibly. The EoS can be better investigated in neutron-rich system if multiple probes are measured simultaneously. For example, the rapidity and the transverse momentum distributions of the charged pions, the π−/π+ ratio, the various pion flow components, as well as the difference of π+ − π− flows. A new sensitive observable is proposed to probe the symmetry potential energy at high densities, namely the transverse momentum distribution of the elliptic flow difference .
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