Interface Orientation Research Articles

Development of an Oral Epithelial Ex Vivo Organ Culture Model for Biocompatibility and Permeability Assessment of Biomaterials.

In the present study, a customized device (Epi-ExPer) was designed and fabricated to facilitate an epithelial organ culture, allowing for controlled exposure to exogenous chemical stimuli and accommodating the evaluation of permeation of the tissue after treatment. The Epi-ExPer system was fabricated using a stereolithography (SLA)-based additive manufacturing (AM) method. Human and porcine oral epithelial mucosa tissues were inserted into the device and exposed to resinous monomers commonly released by dental restorative materials. The effect of these xenobiotics on the morphology, viability, permeability, and expression of relevant markers of the oral epithelium was evaluated. Tissue culture could be performed with the desired orientation of air-liquid interface (ALI) conditions, and exposure to xenobiotics was undertaken in a spatially guarded and reproducible manner. Among the selected monomers, HEMA and TEGDMA reduced tissue viability at high concentrations, while tissue permeability was increased by the latter. Xenobiotics affected the histological image by introducing the vacuolar degeneration of epithelial cells and increasing the expression of panCytokeratin (pCK). Epi-ExPer device offers a simple, precise, and reproducible study system to evaluate interactions of oral mucosa with external stimuli, providing a biocompatibility and permeability assessment tool aiming to an enhanced in vitro/ex vivo-to-in vivo extrapolation (IVIVE) that complies with European Union (EU) and Food and Durg Administration (FDI) policies.

High-strength TiO2/TPU composite fiber based textiles for organic pollutant removal

Effective integration of nanostructured photocatalysts into polymer matrices is crucial for the broad practical application of fiber-based photocatalytic composite textiles. Herein, highly durable TiO2/TPU composite fibers with high TiO2 content (>30 wt. %) were prepared through wet spinning using 1D electrospun TiO2 nanofibers (TNFs). Due to the fiber reinforcement principle, the TNF/TPU composite fibers exhibited superior mechanical properties compared with pure TPU fibers. Furthermore, the TNF/TPU composite fibers with a ratio of 1:2 process impressive degradation efficiency for rhodamine B (RhB). The orientation of 1D TNFs and stronger interface between TNF and TPU matrices not only enable the excellent load sharing and transfer effects following fiber pullout and crack bridging mechanisms, but also improve photogenerated charge transfer efficiency, resulting in high strength and high photocatalytic activity. In addition, enhanced TNF exposure on the fiber is due to the hollow and porous structures of composite fibers, which is advantageous for photocatalytic degradation. Notably, when the RhB concentration exceeded 15 ppm, the TNF/TPU composite fibers exhibited higher degradation efficiency than the TNF powder. Therefore, TNF/TPU composite fiber–based textiles with high photocatalytic activity and strength are promising for overcoming the separation and recovery issues of nanostructured catalysts in practical wastewater treatment applications.

Analytical equation for camera imaging with refractive interfaces

Camera imaging through refractive interfaces is a crucial issue in photogrammetric measurements. Most past studies adopted numerical optimization algorithms based on refractive ray tracing procedures. In these studies, the camera and interface parameters are usually calculated iteratively with numerical optimization algorithms. Inappropriate initial values can cause iterations to diverge. Meanwhile, these iterations cannot efficiently reveal the accurate nature of refractive imaging. Therefore, obtaining camera calibration results that are both flexible and physically interpretable continues to be challenging. Consequently, in this study, we modeled refractive imaging by employing ray transfer matrix analysis. Subsequently, we deduced an analytical refractive imaging (ARI) equation that explicitly describes the refractive geometry in a matrix form. Although this equation is built upon the paraxial approximation, we executed a numerical experiment that shows that the developed analytical equation can accurately illustrate refractive imaging with a considerable object distance and a slightly tilted angle of the flat interface. This ARI equation can be used to define the expansion center and the normal vector of the flat interface. Finally, we also propose a flexible measurement method to determine the orientation of the flat interface, wherein the orientation can be measured rather than calculated by iterative procedures.

In Situ Formation of SnSe2/SnSe Vertical Heterostructures toward Polarization Selectable Band Alignments.

2D van der Waals (vdW) layered semiconductor vertical heterostructures with controllable band alignment are highly desired for nanodevice applications including photodetection and photovoltaics. However, current 2D vdW heterostructures are mainly obtained via mechanical exfoliation and stacking process, intrinsically limiting the yield and reproducibility, hardly achieving large-area with specific orientation. Here, large-area vdW-epitaxial SnSe2/SnSe heterostructures are obtained by annealing layered SnSe. These in situ Raman analyses reveal the optimized annealing conditions for the phase transition of SnSe to SnSe2. The spherical aberration-corrected transmission electron microscopy investigations demonstrate that layered SnSe2 epitaxially forms on SnSe surface with atomically sharp interface and specific orientation. Optical characterizations and theoretical calculations reveal valley polarization of the heterostructures that originate from SnSe, suggesting a naturally adjustable band alignment between type-II and type-III, only relying on the polarization angle of incident lights. This work not only offers a unique and accessible approach to obtaining large-area SnSe2/SnSe heterostructures with new insight into the formation mechanism of vdW heterostructures, but also opens the intriguing optical applications based on valleytronic nanoheterostructures.

Solute segregation, clustering and interphase precipitation in Ti-Mo-Nb microalloyed steel studied by correlated electron backscattering diffraction and atom probe tomography

Site-specific atom probe tomography coupled with electron back scattering diffraction studies were used for the first time to elucidate the multi-element solute segregation and clustering at austenite-ferrite interface during early stages of phase transformation in Fe-Mn-Si-C steel alloyed with Ti, Mo and Nb. The results have shown the existence of negligible partitioning local equilibrium at interfaces with segregation of C, Mn, Ti and Mo to interfaces having the angle deviation in the range from 2.9 to 12° from Kurdjumov-Sacks orientation relationships (K-S ORs), but either irregular incident planes or the ones not following the matching according to K-S ORs. The segregation of Nb was less common, which was linked to the earlier occupancy of interfaces by Ti and Mo. Variation in solute segregation along the same interfaces is highlighted. Presence of clusters within and in close proximity to the interface is detected. The links between interface orientation, solute segregation, clustering and interphase precipitation are discussed.

SPOT-RASTR-A cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface.

In cryogenic electron microscopy (cryo-EM), specimen preparation remains a bottleneck despite recent advancements. Classical plunge freezing methods often result in issues like aggregation and preferred orientations at the air/water interface. Many alternative methods have been proposed, but there remains a lack a universal solution, and multiple techniques are often required for challenging samples. Here, we demonstrate the use of lipid nanotubes with nickel NTA headgroups as a platform for cryo-EM sample preparation. His-tagged specimens of interest are added to the tubules, and they can be frozen by conventional plunge freezing. We show that the nanotubes protect samples from the air/water interface and promote a wider range of orientations. The reconstruction of average subtracted tubular regions (RASTR) method allows for the removal of the nanotubule signal from the cryo-EM images resulting in isolated images of specimens of interest. Testing with β-galactosidase validates the method's ability to capture particles at lower concentrations, overcome preferred orientations, and achieve near-atomic resolution reconstructions. Since the nanotubules can be identified and targeted automatically at low magnification, the method enables fully automated data collection. Furthermore, the particles on the tubes can be automatically identified and centered using 2D classification enabling particle picking without requiring prior information. Altogether, our approach that we call specimen preparation on a tube RASTR holds promise for overcoming air-water interface and preferred orientation challenges and offers the potential for fully automated cryo-EM data collection and structure determination.

Experimental and molecular insights on the regulatory effects of solvent on CaC2 reaction activity

AbstractCalcium carbide (CaC2) is a valuable carbanion resource, but the reactivity is highly restricted by its insolubility and super‐basicity. For this, the effect of solvent and mechanical forces on its reactivity is investigated extensively here via quantum chemistry calculation, molecular dynamic simulation, and experiments. The dissolution free energy of CaC2 in over 100 solvents has been evaluated. DMSO, CH3CN, and DMF can enhance the negative potential and reactivity of CaC2, especially DMSO. The electrostatic interaction of CaC2‐solvent mainly originates from the interaction between Ca2+ and O or N atom. The increased electron density around is mainly ascribed to the electron transfer from solvent. DMSO can change the ionic orientation of CaC2 interface. The solvent may be deprotonated by , compromising the solvent stability. The interface interaction of CaC2 with DMF and DMSO is verified through FT‐IR, and the lattice structure of CaC2 is lost virtually after 0.5 h mechanical milling.

Effects of User Interface Orientation on Sense of Immersion in Augmented Reality

The sense of immersion provided by overlying computer-generated graphics over actual items is one of augmented reality’s distinguishing features. It is affected by multiple factors such as the user interface orientation in the virtual physical hybrid space. There have been many studies on realistic graphic rendering and multimodal interaction in augmented reality. Few studies, however, have systematically investigated the influence of the user interface orientations, more specifically the user-based orientation (UO) and object-based orientation (OO), on various aspects of immersion. We developed the UO and OO user interface prototypes and evaluated the sense of immersion in terms of engagement, engrossment, and overall immersion. The results showed that the user interface orientations in augmented reality had a significant influence on users’ perceived engagement and engrossment, whereas there was no significant difference in overall immersion. Particularly, compared to the OO user interfaces, the UO user interfaces exhibited higher perceived usability and slower focus of attention switching. Generalising implications for alternative augmented reality are provided.

Mechanical anisotropy in additively manufactured laminated high-entropy alloys: The role of interface geometry

The remarkable mechanical properties exhibited by laminated structures have generated significant interest in the realm of additively manufactured laminated high-entropy alloys (HEAs). Despite this burgeoning interest, the nexus between process, structure, and properties within laminated HEAs remains largely uncharted. There is a vast space for investigating the effect of the typical heterogeneous interface on the macroscopic mechanical properties. This study focuses on the influence of the characteristic heterogeneous interface on macroscopic mechanical properties of laminated HEAs, particularly anisotropy. Using the 3D-printed Fe50Mn30Co10Cr10-CoCrNi HEA as a model, we investigate the impact of interface geometry on mechanical characteristics. Tensile tests show that the reduced interface spacing increases yield strength. This laminated HEA displays significant anisotropy in strength and ductility, depending on the loading direction relative to the interface. Electron microscopic observations suggest that finer layer spacing enhances interface and dislocation strengthening, increasing yield strength. Anisotropic behaviors are confirmed to be mediated by interface orientation, explained in terms of deformation compatibility and crack development at the interface. This research offers fundamental insights into the relationship between heterogeneous interfaces and the mechanical properties in laminated HEAs. The knowledge is vital for designing, fabricating, and optimizing laminated HEAs through additive manufacturing, advancing their engineering applications.

The bifunctional low mismatch nano strengthening phase leads to a high strength-ductility W-Ta-Ni-Fe-Cu alloy

As a typical tungsten-based composite consisting of tungsten (W) particles and matrix phase, tungsten heavy alloys (WHAs) are extensively utilized in the military due to their exceptional strength and high density. However, the large grain size (>30 µm) of powder metallurgy liquid phase sintered (LPS) WHAs and the low strength of the matrix phase limit the further improvement of the alloy. In this work, high-density ultrafine WHAs were fabricated by two-step low-temperature sintering, and the density of the alloy reached 17 g/cm3 with an average W particle size of 7.81 µm. Additionally, the eutectic reaction between Ni and Ta was controlled to generate dispersed nano-Ni3Ta phases in situ in the matrix phase, further improving the strength of the alloy. Under the synergistic strengthening effect of fine-grain strengthening, dispersion strengthening, and solid solution strengthening, the average ultimate tensile strength of the alloy reached 1190.39 MPa. At the same time, the alloy maintained good elongation with a total elongation of 20.8 % due to the good co-grid interface orientation between the Ni3Ta phase and the matrix phase. This study provides a new idea for developing high-strength WHAs and has a guiding significance for developing Ni-based alloys.

High temperature fracture behavior of 316L stainless steel-Inconel 718 functionally graded materials manufactured by directed energy deposition: Role of interface orientation and heat treatment

Investigation of functionally graded component consisting of stainless steel 316L (SS316L) and Inconel 718 superalloy (IN718) deposited by laser directed energy deposition (LDED) is presented here. Ambient as well as high temperature mechanical behavior is observed for as-deposited (AD) and for heat-treated (HT) states. The gradient sample was manufactured with the interface orientation parallel and perpendicular to the loading direction to understand the impact of this factor on the materials properties. The evolution of chemical composition, grain structure, phase transformations, and tensile properties at room and elevated temperatures, as well as creep behavior before and after heat treatment was determined. With use of digital image correlation (DIC) analysis, the effects of interface orientation and heat treatment on deformation and fracture mechanism was revealed. Results show that heat treatment smooths the chemical composition and mechanical deformation process, as characterized by the formation of an enhanced gradient diffusion zone in the IN718-rich region near the interface. Heat treatment impacts mechanical properties based on the orientation of the interface. Specimens with interfaces parallel to the load direction combine the strengths of both materials effectively. After HT, they exhibit enhanced mechanical performance, achieving an ultimate tensile strength of 973 MPa and 14% elongation at room temperature. Additionally, at elevated temperatures, the specimen demonstrates exceptional creep resistance, sustaining structural integrity for over 1700 h at 650 °C and 225 MPa. Sample orientation is shown a decisive effect on deformation and fracture mechanism, with the interface not consistently being the weakest point across various gradient samples.

Development of a modified thermal phase change model based on the interfacial area concentration determined by an interface reconstruction algorithm

In order to enhance the physical basis of the mass transfer model in numerical simulation of gas-liquid direct contact condensation and improve the fidelity, a set of methods and models are developed. Based on the concept of the piecewise-linear interface calculation (PLIC), an interface reconstruction algorithm is proposed to calculate the interfacial area concentration in three-dimensional non-orthogonal structured grids. The actual interface in the cell is replaced by a plane, whose position is determined iteratively to satisfy the local volume fraction and orientation of the actual interface. The area of the polygonal cut by the plane is taken as the interfacial area in the cell. The proposed algorithm is validated by calculating the surface area of a series of basic geometries. Obvious superiority of the proposed algorithm over the traditional models (gradient model and algebraic model) is seen. Then, the reconstructed area is used to determine the interfacial area concentration in the thermal phase change model. The process is implemented by ANSYS Fluent user-defined functions. The Stefan problem and steam bubble condensation experiment are used to test the performance of the developed methods and models. The simulation results agree well with the theoretical and experimental results with the quantitative relative errors no more than 4.3 % and 15 %, respectively. The oxygen jets condensation experiments are also used as test cases. The primary dominant frequencies predicted by the simulations are 163.01 Hz and 131.65 Hz, which agree well with the 139.72 Hz and 125.75 Hz in the experiments. The relative errors are only 17 % and 4.7 %. The unstable flow pattern of oxygen jet in liquid oxygen crossflow is explained based on the dynamic characteristics of turbulence, heat and mass transfer. The periodic variation of mass transfer rate is considered as the cause of condensation oscillation. The method for calculating the interfacial area proposed in this paper can serve as an optional and practical improvement to the numerical simulation of direct contact condensation.

Role of interfaces on the mechanical response of accumulative roll bonded nanometallic laminates investigated via dislocation dynamics simulations

Unraveling the effects of continuous dislocation interactions with interfaces, particularly at the nanometer length scales, is key to a broader understanding of plasticity, to material design and to material certification. To this end, this work proposes a novel discrete dislocation dynamics-based model for dislocation interface interactions tracking the fate of residual dislocation on interfaces. This new approach is used to predict the impact of dislocation/interface reactions on the overall mechanical behavior of accumulative roll bonded nanometallic laminates. The framework considers the dynamic evolution of the interface concurrent with a large network of dislocations, thus, accounting for the local short and long range effects of the dislocations under the external boundary conditions. Specifically, this study focuses on two-phase Fe/Cu nanometallic laminates, and investigates the role of the underlying elastic and plastic contrast of the Fe and the Cu layers on the composite response of the material. Moreover, the role of initial microstructures, resulting from processing is also investigated. Subsequently, the model is used to examine the effect of layer thickness and interface orientation relationship on the residual stresses of the relaxed microstructure. The associated mechanical response of these laminates are compared when loaded under normal direction compression, as well as shear compression. Finally, this work predicts a dominant effect of the layer thickness, as compared to the interface orientation relationship, on the macroscopic response and on the residual stresses of these nanolaminates, while the local dislocation transmission propensity through the interface is significantly influenced by the corresponding orientation relationship.

Microstructure of electronic-grade polycrystalline silicon core-matrix interface

This paper focuses on the problems encountered in the production process of electronic-grade polycrystalline silicon. It points out that the characterization of electronic-grade polycrystalline silicon is mainly concentrated at the macroscopic scale, with relatively less research at the mesoscopic and microscopic scales. Therefore, we utilize the method of physical polishing to obtain polysilicon characterization samples and then the paper utilizes metallographic microscopy, scanning electron microscopy-electron backscatter diffraction technology, and aberration-corrected transmission electron microscopy technology to observe and characterize the interface region between silicon core and matrix in the deposition process of electronic-grade polycrystalline silicon, providing a full-scale characterization of the interface morphology, grain structure, and orientation distribution from macro to micro. Finally, the paper illustrates the current uncertainties regarding polycrystalline silicon.

Controlling a Free Surface With Thermocapillary Flows and Vibrations in Microgravity

Fluid manipulation and control is crucial for space exploration. Motivated by the “Thermocapillary-based control of a free surface in microgravity" (ThermoSlosh) experiment (Salgado Sánchez et al. in Acta Astronautica 205:57–67, 2023), we conduct here a detailed numerical analysis of interfacial dynamics in a two-dimensional cylindrical cell, half-filled with different silicone oils or a fluorinert, and subjected to thermal forcing and vibrations. The effect on the free surface dynamics of the applied temperature difference, vibrational amplitude, fluid viscosity, and contact angle is analyzed; both static and dynamic contact angle models are considered. Results strongly suggest that thermocapillary flows can be used to control the interface orientation within the cell, while supplemental vibrations can be added to increase the system responsiveness. This control can be further improved by using classical proportional-integral-derivative feedback to adjust the cell boundary temperatures in real-time. The proportional and derivative gains of the controller can be selected to optimize the stabilization time and/or energy cost, while the integral contribution is effective in reducing the steady-state error. Overall, the present analysis highlights the potential of using the thermocapillary effect for fluid management in reduced gravity, and evaluates different types of experimental tests that can be executed in the frame of the ThermoSlosh microgravity project.

Interfaces of high- and low-speed large-scale structures in compressible turbulent mixing layers: compressibility effects and structures

Direct numerical simulations of temporally developing mixing layers have been performed to investigate the effects of compressibility on statistics and structures near the interfaces of high- and low-speed large-scale structures (LSSs), covering a range of convective Mach numbers from $M_c=0.2$ to $1.8$ and Taylor Reynolds numbers up to 290. The interfaces of LSSs are directly defined by the isosurface of zero fluctuating streamwise velocity. The characteristic velocity jump at the interfaces grows rapidly in the transition stage and then decreases until reaching a gradual self-similar stage. The evolution of velocity jump is evidently delayed as the convective Mach number increases. The interface layer is formed by the shearing motion of neighbouring LSSs. A small vortical motion characterized by the Kolmogorov scale is induced in the interface layer by shear-dominated outer regions. The strengths of outer shearing motion and central vortical motion are greater at the leading edge, but smaller at the trailing edge, which is also reflected in a larger velocity jump at the leading edge. As the convective Mach number increases, the small-scale vortical structure is obviously suppressed by compressibility. At high convective Mach number $M_c=1.8$ , the compressive shear-dominated outer regions are linked with a sheet-like structure passing through the centre of the expansion region corresponding to a small-scale vortical structure. The compressibility and shearing strength near the interface are highly dependent on the interface orientation.

Understanding of magnetization reversal mechanism of precipitation-hardening samarium-cobalt magnets from micromagnetic simulation

The precipitation-hardening Sm-Co magnets not only have irreplaceable applications under extreme conditions such as high temperatures, but are also the most typical magnets with pinning-controlled magnetization reversal mechanism. However, there have always been two views on this type of pinning, attractive or repulsive. In this article, the modification of the parallel interface to the titled interface, led to occurrence of quasi-repulsive and quasi-attractive pinning modes, by employing micromagnetic simulation methods. The corresponding domain wall energy relationship between the pinning phase and main phase was analyzed for different pinning modes, according to the morphology of domain wall from the simulation results. The analysis results indicated that relatively small difference in domain wall energy between the two phases led to quasi-repetitive and quasi-attractive pinning modes. Our findings can provide a reference for further understanding of the magnetization reversal mechanism of precipitation-hardening Sm-Co magnets. The emphasis on the influence of 1:5/2:17 interface orientation on pinning also provided a perspective on the magnetization reversal mechanism of other nanostructured permanent magnet materials.

Portable platform for leukocyte extraction from blood using sheath-free microfluidic DLD.

Leukocyte count is routinely performed for diagnostic purposes and is rapidly emerging as a significant biomarker for a wide array of diseases. Additionally, leukocytes have demonstrated considerable promise in novel cell-based immunotherapies. However, the direct retrieval of leukocytes from whole blood is a significant challenge due to their low abundance compared to erythrocytes. Here, we introduce a microfluidic-based platform that isolates and recovers leukocytes from diluted whole blood in a single step. Our platform utilizes a novel, sheathless method to initially sediment and focus blood cells into a dense stream while flowing through a tubing before entering the microfluidic device. A hexagonal-shaped structure, patterned at the device's inlet, directs all the blood cells against the channel's outer walls. The focused cells are then separated based on their size using the deterministic lateral displacement (DLD) microfluidic technique. We evaluated various parameters that could influence leukocyte separation, including different focusing structures (assessed both computationally and experimentally), the orientation of the tubing-chip interface, the effects of blood sample hematocrit (dilution), and flow rate. Our device demonstrated the ability to isolate leukocytes from diluted blood with a separation efficiency of 100%, a recovery rate of 76%, and a purity of 80%, while maintaining a cell viability of 98%. The device operates for over 30 min at a flow rate of 2 μL min-1. Furthermore, we developed a handheld pressure controller to drive fluid flow, enhancing the operability of our platform outside of central laboratories and enabling near-patient testing. Our platform can be integrated with downstream cell-based assays and analytical methods that require high leukocyte purity (80%), ranging from cell counting to diagnostics and cell culture applications.

The Effect of Interface Orientation During Uniaxial Tension of Functionally Gradient Materials of a Stainless Steel-Copper System Formed by the Wire-Feed Electron Beam Additive Manufacturing

The Effect of Interface Orientation During Uniaxial Tension of Functionally Gradient Materials of a Stainless Steel-Copper System Formed by the Wire-Feed Electron Beam Additive Manufacturing

Precipitation processes and phase interface structure of Ag3Sn during solidification of SnAg3Cu0.5/Cu solder joint

Ag-containing solder alloys are widely used in high-end electronic soldering processes to improve the reliability of electronic components. This study investigates the precipitation process of Ag3Sn during solidification. The precipitation of β-Sn, Ag3Sn, and η′-Cu6Sn5 in SnAg3Cu0.5/Cu solder joints during solidification was examined using Thermo-Calc (2020a). Furthermore, the crystallographic structure of Ag3Sn/β-Sn and Ag3Sn/η′-Cu6Sn5 was analyzed by transmission electron microscopy. The results showed that, below 217.1 °C, the η-Cu6Sn5 phase begins to precipitate: β-Sn + Ag3Sn+η-Cu6Sn5. At the Ag3Sn/β-Sn interface, the angle between (1‾21‾)Ag3Sn and (101‾)β−Sn planes was measured to be 176.4°, with a mismatch of 26.47 %. The Ag3Sn/Cu6Sn5 interface exhibited a specific crystallographic relationship of [223]Ag3Sn// [1‾12]Cu6Sn5, with an angle between (212‾)Ag3Sn and [402]Cu6Sn5 planes of 101.5°. The growth patterns of β-Sn, Ag3Sn, and Cu6Sn5 phases during isothermal aging were further investigated according to their interface orientation relationship, providing a basis for improving the stability of the soldering process.