Surface biopotential dry electrodes are an important part of long-term healthcare health monitoring systems. During long-term dynamic monitoring, pressure fluctuations between the skin/electrode can affect the acquisition of ECG signals and reduce the stability of the system. In this study, by printing an interactive circuit on the back of the Fructus xanthii-inspired barb structure (FXbs) Ag/AgCl-TPU dry electrodes, the micro-nanocone PANI/PMMA flexible conductive film is encapsulated on the back of the FXbs dry electrode, and a multifunctional sensor and monitoring clothing are constructed, which can synchronously collect pressure signals and ECG signals, and can monitor human movements. In the simulation instrument and human skin surface, the influence of pressure fluctuation on the quality of ECG signal acquisition under different pressure, speed, and motion states is explored. In the simulation instrument, when the change rate of piezoresistive reaches the maximum value of 51.41 %, the fluctuation range of ECG is the smallest, which is 0.99 mV. On the surface of human skin, the change rate of piezoresistive and the fluctuation range of ECG in the turning state reach the maximum, which is 5.02 % and 5.99 mV, respectively. It can provide a reference to study the influence of pressure fluctuation on ECG signals on the surface of human skin, and at the same time, it can provide an experimental method and basis for the study of the dynamic noise mechanism at the electrode/electrolyte interface.