In this work, a biomimetic root-soil-like interfacial phase structure was constructed to improve the weak interfacial bonding of the fibers and resin based on the mechanical interlocking theory. Unidirectional basalt fiber (BF) reinforced high-temperature resistant thermoplastic poly (phthalazinone ether nitrile ketone) (PPENK, Tg = 278 °C) composites with multiscale structures were prepared. By utilizing a whiskerization technique, the zinc oxide nanoarrays (ZnO-NAs) with controllable morphologies grown on the BF surface through a pyrolysis-hydrothermal method. The remarkable field emission properties of the ZnO-NAs allow for visually characterizing the interfacial phase structures. The flexural, interlaminar shear, and interfacial shear strengths of BF-ZnO-PEI-2/PPENK composite were increased by 43 %, 65 %, and 177 %, respectively. Further investigations revealed that the flexural strength retention was 61 % at 250 °C, respectively. This study introduces a novel and viable approach to enhance the interfacial properties of BF-reinforced high-performance thermoplastic composites and expands their applications within high-temperature environments.