Integrated Management Strategies Research Articles

First Report of Chili Anthracnose Caused by Colletotrichum sojae in South Korea.

Chili (Capsicum annuum L.) is an economically important crop worldwide, valued for its culinary uses. In South Korea, anthracnose caused by Colletotrichum spp. including C. truncatum, C. gloeosporioides, C. coccodes, C. acutatum, and C. scovillei incurs on substantial economic loss (Kim et al. 2008; Oo and Oh 2020). In August 2022, somewhat different types of symptoms that was not typical on chilli fruits were observed in a field in Yereonggwang (GPS: 35.2579° N, 126.4742° E), South Korea. The disease symptoms appeared as sunken, necrotic lesions with dense black spore masses forming in concentric rings. The estimated disease incidence the 0.2 ha field showing up to 1% of fruits affected. To isolate the pathogen, six symptomatic chilli fruits were collected. Small pieces (5 mm²) were cut from the margins of the lesions, surface-sterilized in 70% ethanol for 30 sec, followed by 1% sodium hypochlorite for 1 minute, and then rinsed three times in sterile distilled water. The tissue pieces were placed on potato dextrose agar (PDA) plates and incubated at 25°C in the dark. After 3 to 5 days, emerging fungal colonies were sub-cultured to obtain pure isolates. A total of five isolates were obtained and initially identified as Colletotrichum spp. based on morphological characteristics. Seven-day old colonies were initially white, turning light orange with age on PDA. Setae (observed on lesion) were dark brown, verruculose and septate. Conidia were cylindrical, hyaline, and measured 14.8 to 19.9 × 4.2 to 6.5 µm (mean 16.7 × 5.6 μm, n = 70) in size; appressoria were brown to dark brown and irregularly shaped. These morphological characteristics of the isolates agree with those reported for the morphology of C. sojae by Damm et al. (2019). To confirm the identity of the isolates, DNA was extracted and specific gene regions were amplified and sequenced using the following primer sets: ITS (ITS1 and ITS4), GAPDH (GDF1 and GDR1), ACT (ACT-512F and ACT-783R), TUB (T1 and Bt2b), HIS3 (CYLH3F and CYLH3R), and CHS-1 (CHS-79F and CHS-345R). The resulting sequences were deposited in the NCBI GenBank with accession numbers (LC830742 to LC830766). Maximum likelihood phylogenetic analysis using combine sequences of ITS, GAPDH, ACT, TUB, HIS3 and CHS-1 in MEGA X confirmed the isolates as C. sojae, marking the first report of this pathogen on chilli in South Korea, previously known to infect soybean. Pathogenicity tests were conducted on wound and nonwounded healthy and mature-green chili fruits (cv. Bicksita) to confirm the pathogenicity of the isolated C. sojae. The fruits were surface-sterilized using 70% ethanol and then rinsed with sterile distilled water. The fruits were wounded using a sterile needle to facilitate infection. A conidial suspension (1x106 conidia/mL) was prepared from 7-day-old PDA cultures. Each fruit was inoculated by placing a 10 µL drop of the conidial suspension onto the wounded and nonwounded sites (4 to 5) of the wound and unwound fruits, respectively. Control fruits were inoculated with sterile water. A total of 40 fruits per treatment were used and the experiment repeated twice. The fruits were placed in plastic box lined with moist paper towels to maintain high humidity and incubated at 25°C. Anthracnose symptoms developed on the inoculated fruits within 7 days, while control and unwounded fruits remained symptom-free. Colletotrichum sojae was successfully reisolated from the symptomatic fruits, fulfilling Koch's postulates and confirming its role as the causal agent of the disease. Colletotrichum sojae is known to infect Fabaceae species worldwide such as Glycine max, Medicago sativa, Phaseolus vulgaris, Atractylodes ovata and Vigna unguiculata (Damm et al. 2019; Talhinhas and Baroncelli 2021), Atractylodes ovata in South Korea (Hassan et al. 2021) and chili pepper in China (Zhanget al. 2023). The first report of C. sojae causing chili anthracnose in South Korea represents a new challenge for chili growers. Integrated disease management strategies need to be developed and implemented to mitigate its impact.

Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean

AbstractAfrican yam bean (AYB; Sphenostylis stenocarpa) is an underutilized legume indigenous to Africa with great potential to enhance food security and offer nutritional and medicinal opportunities. However, low grain yield caused by fungal diseases, including pod blight and leaf tip dieback, deters farmers from large‐scale cultivation. To determine the prevalence of fungal diseases affecting leaves, pods and flowers of AYB, a survey was conducted in 2018 and 2019 in major AYB‐growing areas in Nigeria. Leaf tip dieback, flower bud rot and pod blight were the most common symptoms. Morphological and molecular assays were conducted to identify the causal agents of the observed diseases. In all the samples examined, fungi from eight genera were isolated from diseased leaves, buds and pods. Koch's postulates were fulfilled only for fungi belonging to the Colletotrichum genus. Fungi from the other seven genera did not produce disease symptoms in healthy AYB tissues. Several Colletotrichum isolates were characterized by sequencing the rDNA internal transcribed spacer (ITS), glyceraldehyde‐3‐phosphate dehydrogenase, calmodulin and ApMAT loci. A combined phylogenetic analysis revealed four Colletotrichum species: C. siamense, C. theobromicola and C. fructicola, which were recovered from diseased leaves, and C. truncatum, recovered from diseased pods and buds. Our results are useful to gear efforts to develop integrated management strategies to control diseases affecting AYB in Nigeria and elsewhere. Availability of such strategies may stimulate greater AYB cultivation, which can contribute to diet diversification, something repeatedly advocated by a range of stakeholders to increase food security and prosperity of smallholder farmers.

Seasonal Variation in Nematode Infestation Across Bahar Treatments in Pomegranate Cultivation

This study investigates the influence of three Bahar treatments—Ambe Bahar (spring flowering), Mrig Bahar (monsoon flowering), and Hasta Bahar (autumn flowering)—on the extent of nematode infestation in pomegranate orchards. The research was conducted in major pomegranate-growing regions of India, where soil samples were collected from fields practicing each of the Bahar treatments. The results revealed significant differences in nematode infestation levels across the three Bahar treatments. Mrig Bahar exhibited the lowest nematode density, with an average of 80 nematodes per 100g of soil, compared to 120 in Hasta Bahar and 150 in Ambe Bahar. Additionally, Mrig Bahar treatment was associated with the highest average yield (12.8 kg per plant) and superior fruit quality (Grade A). The findings suggest that Mrig Bahar offers a more conducive environment for nematode management, likely due to its timing relative to environmental conditions that may suppress nematode activity. The results revealed significant differences in nematode infestation levels across the three Bahar treatments. Mrig Bahar exhibited the lowest nematode density, with an average of 80 nematodes per 100g of soil, compared to 120 in Hasta Bahar and 150 in Ambe Bahar. Additionally, Mrig Bahar treatment was associated with the highest average yield (12.8 kg per plant) and superior fruit quality (Grade A). The findings suggest that Mrig Bahar offers a more conducive environment for nematode management, likely due to its timing relative to environmental conditions that may suppress nematode activity. This study underscores the importance of selecting the appropriate Bahar treatment to mitigate nematode infestation and optimize pomegranate production. The insights gained from this research can guide farmers in choosing the most effective cultivation practices, ultimately contributing to sustainable pomegranate farming and improved economic outcomes. Further research is recommended to explore the underlying mechanisms that confer resistance in Mrig Bahar and to develop integrated nematode management strategies tailored to specific Bahar treatments.

Enhancing Healthcare Delivery through Integrated Management Strategies: A Multi-Sector Approach

Enhancing Healthcare Delivery through Integrated Management Strategies: A Multi-Sector Approach

Utilizing machine learning to evaluate heavy metal pollution in the world's largest mangrove forest

The world's largest mangrove forest (Sundarbans) is facing an imminent threat from heavy metal pollution, posing grave ecological and human health risks. Developing an accurate predictive model for heavy metal content in this area has been challenging. In this study, we used machine learning techniques to model sediment pollution by heavy metals in this vital ecosystem. We collected 199 standardized sediment samples to predict the accumulation of eleven heavy metals using ten different machine learning algorithms. Among them, the extremely randomized tree model exhibited the best performance in predicting Fe (0.87), Cr (0.89), Zn (0.85), Ni (0.83), Cu (0.87), Co (0.62), As (0.68), and V (0.90), achieving notable R2 values. On the other hand, the random forest outperformed for predicting Cd (0.72) and Mn (0.91), whereas the decision tree model showed the best performance for Pb (0.73). The feature attribute analysis identified FeV, CrV, CuZn, CoMn, PbCd, and AsCd relationships resembled with correlation coefficients among them. Based on the established models, the prediction of the contamination factor of metals in sediments showed very high Cd contamination (CF ≥ 6). The Moran's I index for Cd, Cr, Pb, and As were 0.71, 0.81, 0.71, and 0.67, respectively, indicating strong positive spatial autocorrelation and suggesting clustering of similar contamination levels. Conclusively, this research provides a comprehensive framework for predicting heavy metal sediment pollution in the Sundarbans, identifying key areas needing urgent conservation. Our findings support the adoption of integrated management strategies and targeted remedial actions to mitigate the harmful effects of heavy metal contamination in this vital ecosystem.

Strategies for falcata (Falcataria falcata (L.) Greuter and R.Rankin) farmers to mitigate gall rust severity across elevations

The extent of gall rust severity in Falcata plantations across various elevations and locations in the country is insufficiently documented, posing challenges for developing an integrated disease management strategy. This study aimed to assess the conditions favorable for gall rust severity in Falcata plantations at different elevations in Mindanao. Our survey revealed that gall rust severity is significantly higher at elevations exceeding 400 m above sea level (asl), with potential yield losses estimated between 23% and 52% of the total log volume per hectare. Factors contributing to increased severity, regardless of elevation, include low stand density (<1000 trees/ha), extended distances from natural vegetation (>1000 m), low understorey species diversity, and low average temperatures (<24°C). These factors interact significantly with elevation, as higher severity was observed in plantations with greater understorey vegetation diversity and elevated temperatures (>24°C), particularly at elevations over 600 m (asl). Based on our findings, it is recommended to avoid planting Falcata in areas with elevations exceeding 400 m (asl). However, if planting at higher elevations is unavoidable, adequate tree spacing (3 m x 3 m or 3 m x 4 m) should be maintained to achieve a moderate stand density (1000-1500 trees/ha). This practice can help regulate air circulation within the plantation, mitigate pathogen spread, and control understory vegetation growth and temperature impacts. Additionally, establishing and maintaining native vegetation or forest within 1000 m around Falcata plantations is advisable to create a protective buffer zone, thereby reducing gall rust severity.

Exploring the Antifungal Activity of Moroccan Bacterial and Fungal Isolates and a Strobilurin Fungicide in the Control of Cladosporium fulvum, the Causal Agent of Tomato Leaf Mold Disease.

The causal agent of tomato leaf mold, Cladosporium fulvum, is prevalent in greenhouses worldwide, especially under high humidity conditions. Despite its economic impact, studies on antifungal agents targeting C. fulvum remain limited. This study evaluates biocontrol agents (BCAs) as alternatives to chemical controls for managing this disease, alongside the strobilurin fungicide azoxystrobin. From a Moroccan collection of potential BCAs, five bacterial isolates (Alcaligenes faecalis ACBC1, Pantoea agglomerans ACBC2, ACBP1, ACBP2, and Bacillus amyloliquefaciens SF14) and three fungal isolates (Trichoderma spp. OT1, AT2, and BT3) were selected and tested. The in vitro results demonstrated that P. agglomerans isolates reduced mycelial growth by over 60% at 12 days post-inoculation (dpi), while Trichoderma isolates achieved 100% inhibition in just 5 dpi. All bacterial isolates produced volatile organic compounds (VOCs) with mycelial inhibition rates ranging from 38.8% to 57.4%. Likewise, bacterial cell-free filtrates significantly inhibited the pathogen's mycelial growth. Greenhouse tests validated these findings, showing that all the tested isolates were effective in reducing disease incidence and severity. Azoxystrobin effectively impeded C. fulvum growth, particularly in protective treatments. Fourier transform infrared spectroscopy (FTIR) analysis revealed significant biochemical changes in the treated plants, indicating fungal activity. This study provides valuable insights into the efficacy of these BCAs and azoxystrobin, contributing to integrated management strategies for tomato leaf mold disease.

Vinegar injections can be used safely to control outbreaks of crown-of-thorns starfish Acanthaster cf. solaris during the peak spawning season

Concerns are mounting over the effects of crown-of-thorns starfish (COTS) (Acanthasteridae) outbreaks, prompting the need for integrated management strategies. Although direct control methods are short-term and localized, they remain one of the few operational tools that can be easily implemented by locals. Vinegar injections have recently emerged as a highly effective method; however, their impact on reproductive behavior remains untested. Here, we investigated the short-term spawning response of mature COTS to double injections of household vinegar. First, COTS abundances and reproductive status were monitored during a massive outbreak affecting New Caledonia’s reefs. In situ and laboratory experiments were then conducted to determine whether injected COTS would trigger synchronized spawning among mature individuals in close proximity. Our results indicated that injections had no significant effect on spawning behavior, even in densely populated aggregations (>4000 COTS ha-1). In the field, starfish exhibited ripe gonads with high gamete content (up to 35% of body weight) 3 d after conspecifics were injected. In the laboratory, mature COTS that were held with injected, decaying individuals in a confined volume did not expel their gametes after 2 d. This suggests that vinegar injections could be used at any time, even during peak spawning, without risking synchronized spawning in the affected areas.

A study of diabetic retinopathy in diabetic foot syndrome in Western Regional Institute of India

Background: Diabetes is a rapidly growing health challenge and potential epidemic across low-and-middle-income countries like India. Diabetic retinopathy (DR) is a consequence of diabetic microangiopathy, which may cause visual deterioration due to macular edema in any stage and vitreous hemorrhage or tractional retinal detachment in the advanced proliferative retinopathy stages. Aims and Objectives: The aim was to study the retinopathy status in diabetic patients with a risk of diabetic foot syndrome (DFS) visiting a western regional hospital in India. Materials and Methods: In this cross-sectional study, all patients with diabetes mellitus (DM) with a risk of DFS, visiting a tertiary care hospital during the study period, underwent an ophthalmological evaluation for documentation of their retinopathy status. Results: One hundred and fourteen patients diagnosed to have a risk profile for DFS were included in the study. Their mean age was 61.22 years and 81.6% were males. The mean duration of DM was 12.24 years, respectively. Of the 114 patients, 72 had DR. An increased presence of retinopathy in patients with an increased risk grade of diabetic foot (DF) was found significant by the Chi‐square test. (P<0.001). Conclusion: Our study found an increased presence of DR in Western Indian cohort with DFS. The severity of retinopathy was greater in patients with higher grades of risk for DF, therefore establishment of an association between DR and DFS will help in developing an integrated management strategy for these two grave consequences of diabetes.

Resistance to protoporphyrinogen oxidase inhibitors in giant ragweed (Ambrosia trifida).

Giant ragweed (Ambrosia trifida L.) is one of the most troublesome weed species in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] cropping systems. Following numerous reports in 2018 of suspected herbicide resistance in several Ambrosia trifida populations from Wisconsin, our objective was to characterize the response of these accessions to acetolactate synthase (ALS), enolpyruvyl shikimate phosphate synthase (EPSPS), and protoporphyrinogen oxidase (PPO) inhibitors applied POST. Four accessions (AT1, AT4, AT6, and AT10) exhibited ≥ 50% plant survival after exposure to the cloransulam 3× rate. Two accessions (AT8 and AT10) and one accession (AT2) exhibited ≥ 50% plant survival after exposure to glyphosate and fomesafen 1× rates, respectively. The AT10 accession exhibited multiple resistance to cloransulam and glyphosate. The AT12 accession was 28.8-fold resistant to fomesafen and 3.7-fold resistant to lactofen. A codon change in PPX2 conferring a R98L substitution was identified as the most likely mechanism conferring PPO-inhibitor resistance. To our knowledge, this is the first confirmed case of PPO-inhibitor resistance in Ambrosia trifida globally and we identified the genetic mutation likely conferring resistance. Proactive and diversified integrated weed management strategies are of paramount importance for sustainable long-term Ambrosia trifida management. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A.

No current technology can specifically target grass carp (Ctenopharyngodon idella) for control within aquatic ecosystems. Rotenone and Carbon Dioxide-Carp are currently the only available registered pesticides for grass carp; they are nonselective and typically applied throughout the water, equally exposing target and native species. A more selective control tool or pesticide application could be used by resource managers to support mitigation efforts. Development of delivery systems that exploit carp feeding strategies could increase selectivity of pesticides and minimize effects on native fishes. A pesticide with selective delivery could be less labor intensive and used within an integrative pest management strategy. The present study examined Antimycin A toxicity in juvenile and sub-adult grass carp and rainbow trout (Oncorhynchus mykiss) across two routes of exposure. Water-based toxicity studies were used to calculate the concentration to cause lethality in 50% of treated fish (LC50) at 24-h, while oral gavage toxicity studies were used to calculate the dose to cause lethality in 50% of treated grass carp and rainbow trout (LD50) 24- to 96-h. Although rainbow trout were more sensitive than grass carp to Antimycin A through water-based exposure, oral toxicity was similar between species, even with inherent gastrointestinal morphological differences. Successful delivery of a lethal dose of Antimycin A to grass carp was achieved through an oral route of exposure using the rapeseed bait and shows promise for registration as a control tool and eventual use in pest management plans. Although a lethal dose of Antimycin A could be incorporated into a single bait pellet, more bait was required to achieve desired mortality when fed to fish under laboratory conditions.

Modern approaches to understanding pathogenetic link between arterial hypertension and renal failure from the point of view of evidence based medicine

Background: Renal failure and arterial hypertension are critical global health issues affecting millions worldwide. Hypertension, affecting over 1.13 billion individuals, is a significant risk factor for cardiovascular and renal diseases. Chronic kidney disease (CKD), affecting approximately 10% of the global population, significantly impacts morbidity, mortality, and socioeconomic wellbeing. The reciprocal relationship between hypertension and renal failure exacerbates these conditions, creating a detrimental cycle of worsening health. Methods and Materials: This study employs a comprehensive review of epidemiological data, pathophysiological mechanisms, and evidence-based management strategies. The research focuses on the bidirectional link between arterial hypertension and renal failure, analyzing the shared pathophysiological pathways such as endothelial dysfunction, inflammation, oxidative stress, and the activation of the renin-angiotensin-aldosterone system (RAAS). Data from various clinical trials and epidemiological studies are synthesized to propose effective treatment and management plans. Results: The findings indicate a strong reciprocal correlation between arterial hypertension and renal failure. Hypertension is prevalent in over 85% of CKD patients, and individuals with hypertension have a significantly higher risk of developing CKD. Shared pathophysiological mechanisms, such as endothelial dysfunction, inflammation, oxidative stress, and RAAS activation, contribute to the progression and worsening of both conditions. Effective management requires addressing these common mechanisms through evidence-based treatments like RAAS inhibitors and lifestyle modifications. Conclusion: The study underscores the need for integrated management strategies targeting both arterial hypertension and renal failure. Early detection, prevention, and comprehensive treatment approaches are crucial for mitigating the health and socioeconomic impacts of these interrelated diseases. Future research should focus on novel therapeutic strategies and personalized medicine to improve patient outcomes and reduce the global healthcare burden.

Integrative metagenomic dissection of last-resort antibiotic resistance genes and mobile genetic elements in hospital wastewaters

Hospital wastewater is a critical source of antimicrobial resistance (AMR), which facilitates the proliferation and spread of clinically significant antimicrobial resistance genes (ARGs) and pathogenic bacteria. This study utilized metagenomic approaches, including advanced binning techniques, such as MetaBAT2, MaxBin2, and CONCOCT, which offer significant improvements in accuracy and completeness over traditional binning methods. These methods were used to comprehensively assess the dynamics and composition of resistomes and mobilomes in untreated wastewater samples taken from two general hospitals and one cancer hospital. This study revealed a diverse bacterial landscape, largely consisting of Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria, with notable variations in microbial composition among hospitals. Analysis of the top 15 genera showed unique microbial pattern distribution in each hospital: Aeromonas was predominant in 1stHWTS (49.39 %), Acidovorax in the CAHWTS at 16.85 %, and Escherichia and Bacteroides in the 2ndHWTS at 11.44 % and 11.33 %, respectively. A total of 114 pathogenic bacteria were identified, with drug-resistant Aeromonas caviae and Escherichia coli being the most prevalent. The study identified 34 types and 1660 subtypes of ARGs, including important last-resort antibiotic resistance genes (LARGs), such as blaNDM, mcr, and tet(X). Using metagenomic binning, this study uncovered distinct patterns of host-resistance associations, particularly with Proteobacteria and Firmicutes. Network analysis highlighted the complex interactions among ARGs, mobile genetic elements (MGEs), and bacterial species, all contributing to the dissemination of AMR. These findings emphasize the intricate nature of AMR in hospital wastewater and the influence of hospital-specific factors on microbial resistance patterns. This study provides support for implementing integrated management strategies, including robust surveillance, advanced wastewater treatment, and strict antibiotic stewardship, to control the dissemination of AMR. Understanding the interplay among bacterial communities, ARGs, and MGEs is important for developing effective public health measures against AMR.

Prevalence of Schistosomiasis and Associated Risks Factors among Patients with Urinary Tract Infection Attending Specialist Hospital Bauchi

Background: Schistosomiasis, a parasitic disease caused by Schistosoma species, remains a significant public health concern in many developing regions, including Bauchi, Nigeria. This study aims to assess the prevalence of schistosomiasis and identify associated risk factors among patients with urinary tract infections (UTIs) attending the Specialist Hospital Bauchi. Methods: A cross-sectional study was conducted involving patients presenting with UTI symptoms at the Specialist Hospital Bauchi. Urine samples were collected and analyzed for Schistosoma haematobium eggs using microscopy. Demographic and clinical data were obtained through structured questionnaires to identify potential risk factors for schistosomiasis, including water contact activities, sanitation practices, and socio-economic status. Results: Out of the total patients screened, a significant proportion tested positive for Schistosoma haematobium, indicating a notable prevalence of schistosomiasis among UTI patients. Statistical analysis revealed several risk factors strongly associated with schistosomiasis, including frequent contact with contaminated water sources, poor sanitation facilities, and lower socio-economic status. Conclusion: The study highlights a high prevalence of schistosomiasis among UTI patients in Bauchi, underscoring the need for integrated disease management and prevention strategies. Public health interventions focusing on improving water sanitation, health education, and targeted treatment programs are essential to reduce the burden of schistosomiasis in this region. These findings can stimulate further research and policy development aimed at controlling this neglected tropical disease.

Integrated management strategies increased silage maize yield and quality with lower nitrogen losses in cold regions.

High-yield and high-quality production of silage maize in cold regions is crucial for ensuring the sustainable development of livestock industry. This study first conducted an experiment to select the optimized silage maize varieties and densities using a split-plot design. The tested maize varieties were Xuntian 3171, Xuntian 16, Xunqing 858, and Fengtian 12, with each variety planted at densities of 67,500, 79,500, and 90,000 plants ha-1. Following the variety and density selection, another experiment on optimizing nitrogen management for silage maize was carried out using a completely randomized design: no nitrogen fertilizer (T1), applying urea-N 320 kg ha-1 (T2), applying urea-N 240 kg ha-1 (T3), applying polymer-coated urea-N 240 kg N ha-1 (T4), and ratios of polymer-coated urea-N to urea-N at 9:1 (T5), 8:2 (T6), 7:3 (T7), and 6:4 (T8). T5-T8 all applied 240 kg N ha-1. The yield and quality of silage maize, nitrogen use efficiency and balance, and economic benefits were evaluated. Results showed that Xunqing 858 had significantly higher plant height (8.7%-22.6% taller than the other three varieties) and leaf area (30.9% larger than Xuntian 3171), resulting in yield 11.5%-51.6% higher than the other three varieties. All varieties achieved maximum yields at a planting density of 79,500 plants ha-1. Integrated management strategy 7 (T7: Xunqing 858, 79,500 plants ha-1, polymer-coated urea-N to urea-N ratio of 7:3) achieved the highest yield of 73.1 t ha-1, a 6.1%-58.1% increase over other treatments. This strategy also produced the highest crude protein (11.1%) and starch (19.1%) contents, and the lowest neutral detergent fiber content (50.6%), with economic benefits improved by 10.3%-97.8% compared to other strategies. Additionally, T7 improved nitrogen use efficiency by 15.4%-94.5%, reduced soil nitrate leaching by 4.4%-36.5%, and decreased nitrogen surplus by 7.0%-46.6%. Comprehensive analysis revealed that the integrated management strategy 7 significantly improved silage maize yield and quality in cold regions while enhancing nitrogen use efficiency and reducing the risk of nitrate leaching, aligning with green agriculture development requirements. These findings will provide vital theoretical insights and practical guidance for high-yield and high-quality silage maize production in cold regions worldwide.

Testing the efficacy of a newly released fungicide, Sphaerex, for control of Fusarium Head Blight in wheat

Fusarium head blight (FHB) is a destructive disease of wheat and other small-grain cereals. FHB causes reduction in crop yield due to pre-mature bleaching of spikes. Additionally, it deteriorates the crop quality due to the production of mycotoxins. An integrated management strategy is needed to effectively manage this disease. One of the most important strategies in the FHB management toolbox is chemical control. Fungicides start losing their efficacy over time due to the development of fungicide resistance in the pathogens. This necessitates the development and testing of new fungicides with multiple active ingredients or modes of action. In this 3-year study, we evaluated the effectiveness of a new fungicide with multiple active ingredients, Sphaerex (a proprietary mix of metconazole and prothioconazole) in an FHB-susceptible soft red winter wheat cultivar, Shirley in the eastern United States. Sphaerex provided significantly better control over untreated plots for FHB Incidence, FHB Severity, and DON content across all three years. The level of control of FHB and DON by Sphaerex was found to be similar to that provided by other standard FHB fungicides. The yield of the fungicide-treated plots was statistically similar to that of the untreated plots.

Integrated Weed Management: A Comprehensive Review of Conventional, Non-Conventional, and Emerging Strategies for Sustainable Agriculture

This manuscript comprehensively reviews integrated weed management (IWM) strategies, emphasizing the need for sustainable agricultural practices. It explores conventional methods, such as herbicide and mechanical control, and non-conventional techniques, including mulching, cover crops, and thermal methods. The review also delves into emerging technologies like precision agriculture, genomic approaches, and robotic weed control. The discussion is framed around five pillars of IWM: diverse cropping systems, cultivar selection, field and soil management, direct control, and monitoring and evaluation. Environmental and socioeconomic impacts of weed management are considered, highlighting the need for a new paradigm that integrates diverse control tactics to enhance agricultural resilience and sustainability.

Insights into the cashew production system in Guinea-Bissau: implications for agroecosystem sustainability

IntroductionWest Africa, particularly Guinea-Bissau, heavily relies on cashew nut (Anacardium occidentale L.) production, which significantly impacts the countries’ economies. Cashew exhibits remarkable adaptability to impoverished and arid soils. Understanding producers’ socio-economic characteristics is crucial due to their potential influence on crop productivity and household income.MethodsThis study aims to characterize the socio-economic, productive, and phytosanitary aspects of cashew production in Guinea-Bissau, through 151 interviews with producers across all administrative regions of the country.ResultsOur findings reveal that cashew producers are mainly males aged 40 to 60, they typically manage plantations under 5 ha, with the smaller plantation areas located in Gabú, Cacheu, and Bolama. The age of the older trees averaged 31 years, with the highest age found in Biombo. Tree density averaged 286 trees per hectare, with higher values in Cacheu, Bafatá, and Bolama. Cashew nut yield in 2020 averaged less than 0.5 tons per hectare, with farmer-set prices generally lower than government determined prices. Oio, Tombali, and Bolama were the regions most affected by pests, while Oio and Cacheu (North) were the most affected by diseases. Top pests included termites, branch girdler, weaver ants, African grasshopper, and stem borer; while top diseases comprised gummosis, anthracnose, and dieback.DiscussionCharacterization of cashew production system is crucial at both national and regional levels to identify region-specific limitations and strengths, aiding in the formulation of tailored strategies for sustainable production. Moreover, cashew production plays a crucial role in household incomes in Guinea-Bissau, underscoring the necessity of developing integrated management strategies.

Fungal Disease Tolerance with a Focus on Wheat: A Review.

In this paper, an extensive review of the literature is provided examining the significance of tolerance to fungal diseases in wheat amidst the escalating global demand for wheat and threats from environmental shifts and pathogen movements. The current comprehensive reliance on agrochemicals for disease management poses risks to food safety and the environment, exacerbated by the emergence of fungicide resistance. While resistance traits in wheat can offer some protection, these traits do not guarantee the complete absence of losses during periods of vigorous or moderate disease development. Furthermore, the introduction of individual resistance genes into wheat monoculture exerts selection pressure on pathogen populations. These disadvantages can be addressed or at least mitigated with the cultivation of tolerant varieties of wheat. Research in this area has shown that certain wheat varieties, susceptible to severe infectious diseases, are still capable of achieving high yields. Through the analysis of the existing literature, this paper explores the manifestations and quantification of tolerance in wheat, discussing its implications for integrated disease management and breeding strategies. Additionally, this paper addresses the ecological and evolutionary aspects of tolerance in the pathogen-plant host system, emphasizing its potential to enhance wheat productivity and sustainability.

Diabetes and Functional Impairment in Osteoarthritis: Investigating Correlations, Associations, and Progression.

Introduction Diabetes and osteoarthritis (OA) are prevalent chronic conditions, often occurring concurrently and complicating patient management. While the individual impact of each condition on functional impairment is well documented, their combined effect remains poorly understood. This study aims to elucidate the relationship between diabetes and OA-related functional impairment. Methodology This was a cross-sectional study of 290 participants with unilateral knee OA. Their demographic, clinical, and diabetes data were collected. Functional impairment was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index-Center for Rheumatic Diseases (WOMAC-CRD). Statistical analyses investigated the relationships between diabetes, OA severity, and functional impairment. Result Diabetic participants showed significantly worse physical function and overall disability, with lower WOMAC-CRD scores. Mean WOMAC-CRD pain scores were 6.46 (SD = 1.088) and 6.48 (SD = 1.101) for the diabetic and non-diabetic groups, respectively. Mean WOMAC-CRD stiffness scores were 6.48 (SD = 1.101) and 6.56 (SD = 1.083) for diabetic and non-diabetic groups. Diabetic participants had a mean WOMAC-CRD physical function score of 55.93 (SD = 2.484), compared to 64.02 (SD = 2.542) for non-diabetic participants. The mean total WOMAC score was 68.80 (SD = 2.857) for diabetic participants and 77.06 (SD = 2.933) for non-diabetic participants. Longer diabetes duration correlated negatively with physical function and total WOMAC scores. Discussion The findings suggest that diabetes exacerbates functional impairment in OA patients, particularly affecting physical function and overall disability. Chronic inflammation and the accumulation of advanced glycation end-products may contribute to the observed deterioration in joint function. Conclusion Integrated management strategies addressing both diabetes and OA are essential for optimizing patient care.