Ca Intake Research Articles

Mucosal expression of Ca and P transporters and claudins in the small intestine of broilers is altered by dietary Ca:P in a limestone particle size dependent manner.

High calcium (Ca) intake and fine limestone reduces precaecal phosphorus (P) absorption independently of P solubility in broilers. This study aimed to determine whether dietary total Ca: total P ratio (Ca:P) and limestone particle size (LPS) affect gene expression of P transporters in the small intestine. A total of 384 one-day-old Ross 308 male broiler chickens received diets low (0.50), medium (1.00) or high (1.75) in Ca:P containing either fine (160 μm) or coarse (1062 μm) limestone, in a 3×2 factorial arrangement. Expression of Ca- and P-related genes were determined using real-time quantitative PCR (RT-qPCR) in duodenum and jejunum. Increasing dietary Ca:P decreased duodenal calcium-sensing receptor (CaSR), calbindin-D28k (CaBP-D28k), plasma membrane Ca-ATPase 1 (PMCA1) and sodium-coupled P cotransporter type IIb (NaPi-IIb), but not transient receptor potential canonical 1 (TRPC1) mRNA. This effect was greater with fine limestone when Ca:P increased from low to medium, but greater with coarse limestone when increased from medium to high. A similar inhibitory effect was observed for jejunal CaBP-D28k expression where increasing dietary Ca:P and fine limestone decreased CaSR mRNA, while dietary Ca:P decreased TRPC1 mRNA only for coarse limestone. It also decreased jejunal NaPi-IIb mRNA irrespective of LPS. Dietary treatments did not affect jejunal PMCA1 mRNA expression or that of inorganic phosphate transporter 1 and 2 and xenotropic and polytropic retrovirus receptor 1 in both intestinal segments. Dietary Ca increase reduced mucosal claudin-2 mRNA in both segments, and jejunal zonula occludens-1 (ZO-1) mRNA only for coarse limestone. In conclusion, increasing dietary Ca:P reduced expression of duodenal P transporters (NaPi-IIb) in a LPS dependent manner, hence Ca induced reduction in intestinal P absorption is mediated by decreasing P transporters expression. Dietary Ca reduces Ca digestibility by downregulating mRNA expression of both Ca permeable claudin-2 and Ca transporters (CaBP-D28k, PMCA1).

Health-risk Assessment and Evaluation of Different Metals in Different Tea Products Consumed in Jordan Using ICP-MS and ICP-OES

This study aimed to analyze the concentrations of heavy metals and trace elements in black, green, white and oolong tea samples in different brands that are widely consumed in Jordan to assess the health risk associated with their consumption. Inductively Coupled Plasma equipped with Mass Spectrometry and Optical Emission Spectrophoto¬metry (ICP-MS/OES) were used for analysis after sample preparation. The mean concentrations of Cr, Co, Ni, Cu, Zn, As, Se, Sr, Mo, Pb, Sn, Cd, Ca, Fe, K, Mg and Na were found to be in the range of not detected to 135.4 mg/kg and 0.10–389.93 mg/kg for both infused and digested samples, respectively. The daily intake of Ca, K, Na and Mg was found in the range of 0.31–9.03% of their Recommended Dietary Intake (RDA). The Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), Hazard Index (HI) and Target Cancer Risk (TR) were used for the assessment of health risks associated with the metals’ intake. THQ, HI and TR values of all elements were found to be below the allowable limits determined by the World Health Organization (WHO) and the United States Environmental Protection Agent (US-EPA), suggesting that the consumption of different tea products from the Jordanian market does not pose a significant risk to human health.

Diet X Gene Interactions Control Femoral Bone Adaptation to Low Dietary Calcium.

ABSTRACTGenetics and dietary calcium (Ca) are each critical regulators of peak bone mass but it is unclear how genetics alters the physiologic response of bone to dietary Ca restriction (RCR). Here, we conducted genetic mapping in C57BL/6J × DBA/2J (BXD) recombinant inbred mouse lines to identify environmentally sensitive loci controlling whole‐bone mass (bone mineral density [BMD], bone mineral content [BMC]), distal trabecular bone, and cortical bone midshaft of the femur. Mice were fed adequate (basal) or low Ca diets from 4–12 weeks of age. Femurs were then examined by dual‐energy X‐ray absorptiometry (DXA) and micro‐computed tomography (μCT). Body size–corrected residuals were used for statistical analysis, genetic mapping, and to estimate narrow sense heritability (h2). Genetics had a strong impact on femoral traits (eg, bone volume fraction [BV/TV] basal Ca, h2 = 0.60) as well as their RCR (eg, BV/TV, h2 = 0.32). Quantitative trait locus (QTL) mapping identified up to six loci affecting each bone trait. A subset of loci was detected in both diet groups, providing replication of environmentally robust genetic effects. Several loci control multiple bone phenotypes suggesting the existence of genetic pleiotropy. QTL controlling the bone RCR did not overlap with basal diet QTL, demonstrating genetic independence of those traits. Candidate genes underlying select multi‐trait loci were prioritized by protein coding effects or gene expression differences in bone cells. These include candidate alleles in Rictor (chromosome [chr] 15) and Egfl7 (chr 2) at loci affecting bone in the basal or low Ca groups and in Msr1 (chr 8), Apc, and Camk4 (chr 18) at loci affecting RCR. By carefully controlling dietary Ca and measuring traits in age‐matched mice we identified novel genetic loci determining bone mass/microarchitecture of the distal femur as well as their physiologic adaptation to inadequate dietary Ca intake. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

Vitamin D-Mediated Regulation of Intestinal Calcium Absorption.

Vitamin D is a critical regulator of calcium and bone homeostasis. While vitamin D has multiple effects on bone and calcium metabolism, the regulation of intestinal calcium (Ca) absorption efficiency is a critical function for vitamin D. This is necessary for optimal bone mineralization during growth, the protection of bone in adults, and the prevention of osteoporosis. Intestinal Ca absorption is regulated by 1,25 dihydroxyvitamin D (1,25(OH)2 D), a hormone that activates gene transcription following binding to the intestinal vitamin D receptor (VDR). When dietary Ca intake is low, Ca absorption follows a vitamin-D-regulated, saturable pathway, but when dietary Ca intake is high, Ca absorption is predominately through a paracellular diffusion pathway. Deletion of genes that mediate vitamin D action (i.e., VDR) or production (CYP27B1) eliminates basal Ca absorption and prevents the adaptation of mice to low-Ca diets. Various physiologic or disease states modify vitamin-D-regulated intestinal absorption of Ca (enhanced during late pregnancy, reduced due to menopause and aging).

High dietary Ca and microbial phytase reduce expression of Ca transporters while enhancing claudins involved in paracellular Ca absorption in the porcine jejunum and colon.

Expression levels of genes (RT-qPCR) related to Ca and P homeostasis (transporters and claudins (CLDN)) were determined in porcine jejunal and colonic mucosa. Forty growing pigs (BW 30.4±1.3 kg) received a low and high Ca content (2.0 and 9.6 g/kg, respectively) diet with or without microbial phytase (500 FTU/kg) for 21 days. Dietary Ca intake enhanced serum Ca and alkaline phosphatase concentration and reduced P, 1,25(OH)2D3, and parathyroid hormone concentration. Jejunal TRPV5 mRNA expression was decreased (32%) with phytase inclusion only, while colonic transient receptor potential vanilloid 5 (TRPV5) mRNA was reduced by dietary Ca (34%) and phytase (44%). Both jejunal and colonic TRPV6 mRNA expression was reduced (30%) with microbial phytase. Calbindin-D9k mRNA expression was lower in colonic but not jejunal mucosa with high dietary Ca (59%) and microbial phytase (37%). None of the mRNAs encoding the Na-P cotransporters (NaPi-IIc, PiT-1, PiT-2) were affected. Jejunal, but not colonic expression of the phosphate transporter XPR1, was slightly downregulated with dietary Ca. Dietary Ca downregulated colonic CLDN-4 (20%) and -10 (40%) expression while CLDN-7 was reduced by phytase inclusion in pigs fed low dietary Ca. Expression of colonic CLDN-12 tended to be increased by phytase. In jejunal mucosa, dietary Ca increased CLDN-2 expression (48%) and decreased CLDN-10 (49%) expression, while phytase slightly upregulated CLDN-12 expression. In conclusion, compared to a Ca deficient phytase-free diet, high dietary Ca and phytase intake in pigs downregulate jejunal and colonic genes related to transcellular Ca absorption and upregulate Ca pore-forming claudins.

Research Note: Formulating broiler diets using digestible calcium significantly improved growth performance but reduced apparent ileal digestibility of calcium and phosphorus

An experiment was conducted to compare the use of a total and digestible Ca formulation system in broilers from hatch to d 37 post-hatch. Ross 308 (n = 288) male broilers were obtained and allocated to one of 2 dietary treatments in floor pens. There were 18 birds per pen and 8 replicate pens per diet. One diet was formulated using ingredient and nutrient recommendations for total Ca and the second diet was formulated using ingredient and nutrient recommendations for standardized ileal digestible (SID) Ca. All diets were formulated to be nutrient adequate using a 2-phase feeding program and including 0.19% available P (avP) and 0.209% total Ca or 0.073% digestible Ca from 1,000 FYT/kg of phytase. On d 17 and 37, tibias and ileal contents were obtained. From hatch to d 37, birds fed diets formulated using digestible Ca gained (P < 0.05) more and were more efficient (P < 0.05) compared with birds fed diets formulated using total Ca. There was no impact of formulation system on tibia ash or minerals. Litter pH (P < 0.05) was greater and litter dry matter (P < 0.05) was lower in birds fed the diets formulated using digestible Ca compared with those fed diets formulated using total Ca. Apparent ileal digestibility (AID) of Ca (P < 0.05), AID of P (P < 0.05) and digestible P intake (P < 0.05) were lower in birds fed diets formulated using digestible Ca compared with those formulated using total Ca at d 17 or 37. However, apparent ileal digested Ca and digestible Ca intake were not different between the experimental diets on d 17 or 37. In conclusion, formulating diets using digestible Ca improved weight gain and feed conversion ratio, but reduced the AID of Ca and P compared with birds fed diets formulated using total Ca. These findings might be reflective of the higher total Ca concentration in the diets formulated using digestible Ca compared with those formulated using total Ca.

Role of Calcium Supplementation on Pregnancy Induced Hypertension Outcomes

Background: Pregnancy induced hypertension (PIH), is an important cause of both maternal and perinatalmorbidity and mortality. Low dietary calcium (Ca) intake represents a factor associated with an increasedincidence of hypertensive disease in general and in pregnant population in most low and middle income countries.Ca supplementation has the potential to reduce adverse gestational outcomes of PIH.Aim: To assess the effects of low-dose Ca supplementation on PIH outcomes.Method: A single- blind, randomized controlled trial was conducted in January through March, 2021. A totalnumber of 66 pregnant women with PIH at their 20th week of gestation attended maternal care outpatient inBaghdad Medical City were assigned randomly in two groups those received Ca supplementation 500 mg/day vsnone and follow up both groups till delivery for outcomes.Results: A significant reduction in diastolic blood pressure mean (93.28 mmHg vs. 88.62 mmHg, P= 0.015) andalmost significant reduction in systolic blood pressure mean (149.65 mmHg vs. 142.58 mmHg, P= 0.051) in theparticipants after receiving Ca supplements. Pre eclampcia (10.3% vs. 45.9%, P = 0.002), Low birth weight (6.9%vs. 40.5%, P = 0.002), Pre term delivery (10.3% vs. 43.2%, P=0.003), and admissions to neonatal intensive care unit(10.3% vs. 45.9%, P = 0.002) were significantly lower in the intervention group.Conclusion: Ca supplementation of 500 mg daily started at 20th week of gestation associated with reduction ofmaternal blood pressure, pre-eclampcia, preterm delivery, low birth weight and admission to neonatal intensivecare unit especially in a low dietary Ca intake mothers.

Association of daily nutrient intake with breakfast and snack consumption among young Japanese adults aged 20-39 years: data from the 2012 National Health and Nutrition Survey.

Eating frequency has been associated with nutrient intake and diet quality. The aim of this study was to examine the association between daily nutrient and food group intake and consumption of breakfast and/or snacks among young Japanese adults, as secondary analysis of the 2012 National Health and Nutrition Survey in Japan involved 1420 men and 1659 women aged 20-39 years. Dietary intake data were collected using a one-day semi-weighed household dietary record. Participants were classified into four groups based their breakfast and snack consumption, defined as the consumption of any food or beverage that contained energy based on participant-defined eating occasions; both breakfast and snack consumption (B+S+), breakfast consumption without snacking (B+S-), breakfast skipping and snack consumption (B-S+) and breakfast skipping without snacking (B-S-). The proportion of breakfast skippers among men and women was 11·8 % and 6·6 %, whereas that of snack consumers among men and women was 55·3 % and 68·2 %, respectively. Energy, Mg potassium (in both sexes), vitamin B2, Ca, (only men) and folate and dietary fibre (only women) intakes were higher among breakfast and snack consumers than in the B+S- group. The nutrient intake level of the B+S- group was similar to that of the B-S+ group, whereas the B-S- group had lower energy (both sexes), Cu, dietary fibre and potassium (only women) intakes than the B+S- group. Snack consumption could supplement nutrients that may not be adequately consumed by three meals among young Japanese adults.

Knowledge, Beliefs, and Preventive Behaviors Regarding Osteoporosis among University Students: Scoping Review

Context: Osteoporosis is a universally growing health problem. Adequate calcium intake and physical activity, alongside a high level of knowledge and health beliefs, play a crucial role in preventing or delaying diseases.&#x0D; Aim: This scoping review provides an overview of university students' prevailing knowledge, beliefs, and behaviors regarding osteoporosis.&#x0D; Methods: CINHAL, PubMed, and MEDLINE databases were searched to cover the period between 2014 to 2019. Twenty-seven articles were selected out of 4078 were retrieved. The following searching terms were used: (osteoporosis knowledge OR osteoporosis awareness) AND osteoporosis beliefs AND (osteoporosis practices OR osteoporosis behaviors OR calcium intake OR physical activity) AND (osteoporosis OR bone mineral density) AND (nursing students OR university students OR undergraduate students OR college-age students OR young adults). Finally, the level of osteoporosis knowledge, health beliefs, preventive behaviors, the relationship between practices/behaviors and lifestyle with bone health, and the educational intervention's significant/nonsignificant positive effect on the students' osteoporosis knowledge, the prevailed beliefs, and the level of engagement in the osteoporosis preventive behaviors were documented.&#x0D; Results: Most articles demonstrate that the students' osteoporosis knowledge was insufficient and seriously lacking in osteoporosis's perceived susceptibility and severity. This finding clarified that most students were not adequately engaging in the osteoprotective preventive behaviors (Physical activity ad Ca intake). Also, the finding indicates that following a healthy lifestyle and engaging in osteoprotective behaviors positively affect BMD and vice versa.&#x0D; Conclusion: The lack of osteoporosis awareness among the university students and the low perception of osteoporosis susceptibility and severity explains why most participants are not engaged in osteoporosis preventive behaviors due to the strong influence of personal beliefs, perceptions, and misconceptions. Therefore, it is important to design educational programs targeting these groups to raise awareness and change their subsequent beliefs.

Influence of Calcium and Nonphytate Phosphorus (NPP) on Meat-Type Quail’s Growth, Carcass Features, and Tibia Indices

This study aimed to evaluate the role of increasing dietary calcium (Ca) and non-phytate phosphorus (NPP) supplementation on the growth, carcass, edible portions, and tibia indicators of growing quail. The current study was conducted in a 3×3 factorial design, for this, 360 1-wk-old quails were haphazardly assigned to nine groups, each group is with three gradual levels of Ca (0.60, 0.90, and 1.20 %) and NPP (0.20, 0.40 and 0.60 %). Each group was divided into five replicates with eight-quail each. Results of the study suggested that except at 2 and 6 weeks of age, dietary Ca level did not exhibit any significant (P &gt; 0.05) impact on body weight. Similarly, in the case of NPP, apart from the live weight at 2 and 3 weeks of age, NPP did not have a significant impact on live body weight. Further, in comparison to the low Ca level, the moderate or high Ca levels have higher values of body weight gain. During all the experiments, dietary Ca, NPP, or their mixtures had no significant (P &gt; 0.05) impact on feed consumption. Similarly, feed conversion rate and carcass metrics were also not affected by the individual or combined application of Ca or NPP supplementation Similarly, dietary intakes of Ca or P did not have any significant effect on the various tibia indicators (P &gt; 0.05). Results of the study can be concluded that the effect of the Ca and NPP levels in Japanese quail diets is lowered and it does not much affect the growth rate, feed utilization, carcass yields, edible components, or tibia indices.

Pre-diagnostic dietary consumption of calcium and magnesium and calcium-to-magnesium intake ratio and ovarian cancer mortality: results from the ovarian cancer follow-up study (OOPS).

Previous studies have indicated that dietary consumption ofcalcium (Ca), magnesium (Mg), and the Ca-to-Mg (Ca:Mg) ratio were associated with different health outcomes. However, no study has evaluated the association of pre-diagnostic Ca, Mg, and Ca:Mg ratio consumption with ovarian cancer (OC) survival. The aforementioned associations were investigated in a cohort of 853 Chinese women diagnosed with OC between 2015 and 2020. A validated food frequency questionnaire was used to evaluatepre-diagnostic diet information. Deaths were recorded until March 31, 2021 via medical recordsand active follow-up. Cox proportional hazards model was applied to calculate the adjusted hazard ratios (HRs) and 95% confidence intervals (CIs). A total of 130 deaths were observed during a median follow-up of 37.2months. After adjustment for potential confounders, pre-diagnostic Ca (HR<600 vs. >1000 = 1.45, 95% CI = 0.47-4.46, p for trend = 0.69) and Mg (HR< 250 vs. >330 = 0.90, 95% CI = 0.39-2.08, p for trend = 0.77) intakes were found to be unrelated to OC survival, whereas a higher Ca:Mgintake ratio was significantly associated with worse survival (HR< 1.7 vs. > 2.5 = 2.72, 95% CI = 1.28-5.78, p for trend < 0.05). A significant result was also observed when treating the Ca:Mg ratio as a continuous variable (HR = 1.69, 95% CI = 1.12-2.55) for one-unit increment. Pre-diagnostic consumption of Ca and Mg was unrelated to OC survival, while a higher Ca:Mg intakeratio was strongly associated with worse survival among OC patients.

A population based case–control study of association between dietary calcium intake and ulcerative colitis in adults

Limited data are available on the association of dietary calcium intake and ulcerative colitis (UC). We aimed to investigate the relation between dietary calcium intake and UC prevalence in Iranian adults. In this population-based case–control study, diagnosed patients with UC by gastroenterologists that were registered in the Iranian inflammatory bowel disease registry were included as cases. Age and sex-matched healthy controls were selected from Study on the Epidemiology of Psychological, Alimentary Health and Nutrition (SEPAHAN) dataset. Dietary calcium intakes of participants were examined through a validated food frequency questionnaire. We included 327 middle-aged participants (109 cases and 218 controls) in the analysis; 52.1% of them were females. After adjustments for potential confounders, individuals in the third tertile of dietary calcium intake had 92% lower odds of UC, compared to those in the first tertile (OR = 0.08, 95% CI 0.02–0.27). Our analysis based on recommended dietary allowances (RDAs) intake showed that dietary Ca intake deficiency was related to increased odds of UC (OR = 9.5, 95% CI 2.98–30.91). Stratified analysis by gender revealed that these associations were significant in both genders; although the results were stronger in the male population. A Significant decreasing trend was observed for odds of UC in tertiles of dietary calcium intakes, in both males and females. Higher dietary calcium intake was associated with lower UC prevalence in Iranian adults. Inadequate dietary calcium intake was also linked to elevated odds of UC. Further prospective investigations are needed to affirm these findings.

Does Vitamin D Insufficiency Influence Prebiotic Effect on Calcium Absorption and Bone Retention?

Higher calcium (Ca) absorption would partially compensate for Ca intake below requirements for bone health. Previously, we found that GOS/FOS prebiotic mixture (PM) increases Ca absorption in the colon and retention in bone. Ca absorption and retention are regulated by vitamin D (VD). Hence, it is relevant to explore whether VD insufficiency influences the effect of the PM in the colon. The effect of the PM on Ca, phosphate (IP), and magnesium (Mg) absorption and retention under conditions of VD sufficiency and insufficiency (VDInsuff) was compared using a preclinical model of VDInsuff and low bone mass. Ovariectomized rats were fed isocaloric semisynthetic diets according to AIN-93M. The diets varied in Ca (0.5% or 0.3%), VD [100IU% (+ D) or 0IU% (- D)], and PM (2.5% or 0%) content. The following eight groups were studied: + D0.5; + D0.3; + DPM0.5; + DPM0.3; - D0.5; - D0.3; - DPM0.5; and - DPM0.3. Irrespective of Ca content, VDInsuff did not affect the prebiotic effect of the PM on caecum pH, lactobacillus colony growth, or Mg absorption but significantly decreased its effect on colonic crypt length and cell/crypt and Ca and IP absorption. The PM failed to counterbalance the pro-inflammatory effect of VDInsuff. Moreover, bone retention i.e., bone mineral content and density, bone volume, and bone quality parameters were significantly lower (p < 0.05) and bone turnover significantly was higher (p < 0.05). Although the PM is a useful tool to improve mineral absorption and bone retention, it would seem important to monitor VD nutritional status to ensure the full prebiotic effect in the large intestine.

When Incorporated into Fruit Sorbet Matrix, Are the Fructans in Natural Raw Materials More Beneficial for Bone Health than Commercial Formulation Added Alone?

Simple SummaryBecause of the serious consequences of osteoporosis, the prevention of this disease, which is also known as “the Silent Epidemic”, is considered essential to the maintenance of human health. It is crucial to maximize bone mass and strength during childhood and adolescence to reduce the risk of osteoporosis in later periods of life. The optimization of nutrition is one of the most important factors. Special attention should be paid to ensuring sufficient calcium intake, which is a problem in several population groups, and its bioavailability. We assessed the effect of fructans, taking into consideration their source (Jerusalem artichoke, yacon, commercial preparation) and the form of their addition to the diet (alone or as an ingredient of strawberry sorbet). A growing-female-rat model was used to assess the potential functional properties of these non-digestible carbohydrates in/without the “fruit matrix”, during calcium hypoalimentation. We showed that fructans have a protective effect during the inadequate supply of Ca, but their action is related to their source as well as the dietary matrix. It is important to look for new preventive strategies, and our paper gives an insight into various fructan sources, depicting their action and how these natural dietary components can be exploited.We assessed the extent to which fructans from various sources and added in various forms (raw materials in diet alone or incorporated into a strawberry matrix) differ in their effectiveness towards selected parameters related to bone health under calcium hypoalimentation in growing female Wistar rats. The aim of this study was to evaluate the levels of selected parameters involved in calcium metabolism, in response to a 12-week restriction of Ca intake: serum ions (Ca, Mg, P); the activity of alkaline phosphatase—using a BS 120 analyzer; the markers of bone turnover (osteocalcin, CTX; using a Rat-MidTMOsteocalcinEIA Kit and RatLapsTMEIA, respectively); and the bone mineral content (BMC) and density (BMD), using a Norland Excell Plus Densitometer. Among the examined markers, the CTX concentration increased dramatically under calcium hypoalimentation. The presence of Jerusalem artichoke (independently of the form of addition) and yacon root powder (with strawberry sorbet matrix) in the rats’ diet led to a significantly lower CTX concentration than was observed in the low-calcium control group. The type of fructan influenced the bone mass content. When fructan was added to the low-calcium diet as an ingredient of sorbet, it exerted more pronounced effects on the biochemical parameters of bone metabolism than when added alone, in the growing-female-rat model.

Estimation of daily dietary intake of essential minerals and trace elements in commercial complementary foods marketed in Brazil

Eating habits influence health and development throughout life and commercial complementary foods are an important part of the diet for many infants. In this study, Ca, Fe, K, Mn, Mg, Na, Se and Zn were determined in twenty-seven Brazilian commercial complementary foods samples by Instrumental Neutron Activation Analysis. Daily dietary intakes of the elements were estimated in three groups aged 6–8 months, 9–11 months and 12–24 months from breastfed and non-breastfed infants and young children. Non-breastfed children presented higher daily dietary intakes for all elements. Daily dietary intakes of Fe, Mg, Mn, Na and Zn did not meet the recommended values for the 6–8 and 9–11 months for the breastfeeding group. Ca, K, Mg, Mn, Na and Zn dietary intakes were below the recommended values for 12–24 months for breastfeeding group. Se daily dietary intakes were inadequate in all studied groups. No investigated element exceeded the upper limits. It was observed that the commercial infant food analyzed do not provide sufficient amount of essential minerals and trace elements for the child healthy development in accordance to the international health recommendations.

69 Impact of DON in a Protocol of Calcium Depletion and Repletion in Piglets

Abstract This study aimed to evaluate factors that modulate phosphorus (P) use, such as calcium (Ca) intake that is well known and deoxynivalenol (DON) contamination which has been the subject of few studies until now. A total of 64 piglets (2 piglets per pen) received 1 of 4 treatments distributed into 8 blocks during an initial 13-d phase: control (DON-) or DON-contaminated treatment (DON+, 2.72 mg/kg) with either a low Ca (Ca-, 0.39%) or normal Ca (Ca+, 0.65%) level with a constant digestible P level (0.40%). During a second 14-d phase, piglets that received Ca+ and Ca- were fed a similar diet containing 0.65% Ca and 0.35% P without DON contamination. Growth performance and apparent total tract digestibility (ATTD) of Ca and P for piglets and bone mineral content (BMC) of 1 pig per pen were evaluated. After phase 1, ATTD P was higher for Ca-DON+ (DON x Ca interaction, P &amp;lt; 0.01). The ATTD Ca was higher for DON+ (( P &amp;lt; 0.001). The DON+ increased BMC in Ca+ but opposite effect was observed in Ca- (DON x Ca interaction, P &amp;lt; 0.001). The plasma P concentration was greater for Ca- (P = 0.05). After phase 2, average daily gain (P = 0.08) and average daily feed intake (P = 0.09) tended to be less for Ca- than for Ca+. The Ca- piglets recovered their BMC deficit in DON-, but not those receiving DON+ (DON x Ca interaction, P = 0.06). ATTP P in DON- was lower in Ca+ but increased in DON+ regardless of Ca level (DON x Ca interaction, P = 0.04). Plasma P and Mg were greater for Ca- (P = 0.05). The results show that piglets can regain the BMC caused by Ca depletion in DON non-contaminated feed. DON contamination increased Ca and P digestibility and modified BMC depending on Ca supply.

Validation of an instrument to assess food diversity in women of childbearing age in Medellín, Colombia.

To validate a Food Diversity Questionnaire (CDA, for its name in Spanish) that identifies the prevalence of the risk of deficiency in the intake of eleven micronutrients. The CDA paper form, an online application for data entry and handling, was designed and compared with the 24-h recall (24HR) as a reference method. All data were processed in Personal Computer Software for Intake Distribution Estimation (PC-SIDE) v1 software. A descriptive analysis and comparisons between prevalence, concordance and reproducibility analyses were performed. Medellín, Colombia. Women of childbearing age between 19 and 50 years (n 186) who worked for the Buen Comienzo programme in 2019. When comparing the adjusted 24HR technique and the CDA, there was no significant difference in population-level data at risk of deficiency in any micronutrient intake. However, based on individual-level data of the best linear unbiased predictor, the concordance analyses were weak, and although agreements were high according to the diagnostic performance tests, a good ability to detect deficiency was only observed in a few nutrients: vitamin A 100·0 %, Ca 98·7 %, Fe 92·8 %, folates 91·6 %, and pyridoxine 81·8 %. The CDA validated in this study is useful and faster at evaluating population-level data at risk of deficiency in the intake of Ca, Fe, Zn, thiamine, riboflavin, niacin, pyridoxine, folates, vitamin B12, vitamin C and vitamin A. Based on individual-level data, a good ability to detect deficiencies was observed in the intake of vitamin A, Ca, Fe, folates and pyridoxine.

Significant Nutritional Gaps in Tibetan Adults Living in Agricultural Counties Along Yarlung Zangbo River.

BackgroundDietary intake and nutritional assessing data from a representative sample of adult population living in an agricultural zone on Tibet Plateau are still lacking nowadays. This study aimed to assess the daily dietary intakes and respective food sources in 552 local residents (≥ 18 years old, 277 men and 275 women) living in 14 agricultural counties along the Yarlung Zangbo River on Tibet Plateau.MethodsFood consumption data were collected using a validated cultural-specific food frequency questionnaire that contained all local Tibetan foods and analyzed with three fixed factors: gender, age, and region. Nutrient intakes were calculated using Chinese food composition tables. Nutritional gaps and the percentages of participants who had inadequate and excessive nutrient intakes were calculated by estimated average requirement (EAR) cut-point methods.ResultsCompared with the dietary reference intakes, 68.4% of nutrient intakes were inadequate. Fiber, Ca, I, Zn, Se, and vitamin (Va, Vc, and folic acid) intakes appeared to be particularly deficient. The dietary energy intake was 7838.8 ± 537.1 KJ/d, with 78 and 84% of EAR values for men and women, respectively. The dietary intakes of most nutrients were below the estimated energy requirement/EAR or adequate intake values, while more than 70% of the participants had excessive intake of carbohydrate, especially the elderly (aged ≥ 51 years). The nutritional gap of Cu was more than 300%. Almost 100% of the participants was vulnerable to fiber, Se, and Va shortfalls due to the deficiency in sole food sources. The top five food sources of Se intake were highland barley (34.2%), meat (13%), rice (12.4%), eggs (12.2%), and cultural-specific beverages (7.8%). Eggs (42.1%), tubers (62.2%), vegetables (66.4%), and highland barley (49.7%) were the first contributors of Va, Ve, Vc, and folic acid, respectively.ConclusionThe dietary intake of a large sample of Tibetan adult population living in agricultural counties of Tibetan Autonomous Region is alarmingly insufficient. Gender inequality is common, and regional difference is widespread due to rapid urbanization. Young Tibetan adults aged 18–30 years are particularly vulnerable to micronutrient shortfalls and currently facing the risk of nutrition-insecurity-related dietary inadequacy. The respondents who belong to the elderly category (≥51 years of age) are facing the risk of “double burden of malnutrition” characterized by the coexistence of undernutrition, including micronutrient deficiencies and overweight or obesity.

Dietary oxalate to calcium ratio and incident cardiovascular events: a 10-year follow-up among an Asian population

Background and aimThe potential cardiovascular impact of usual intakes of oxalate (Ox) is uninvestigated. We evaluated the effect of dietary Ox and its interaction with dietary calcium (Ca) on incident cardiovascular disease (CVD).MethodsWe included 2966 adult men and women aged 19–84 y without known CVD during baseline enrollment (2006–2008) of the Tehran Lipid and Glucose Study. Dietary intakes were assessed using a validated FFQ, and incident CVD (i.e., coronary heart disease, stroke, and CVD mortality) were documented through March 2018.ResultsA 7.1% incident of CVD occurred during a median follow-up of 10.6 y. After multivariable adjustment for traditional risk factors and key dietary nutrients, including total fat and fiber, Ox intakes ≥220 mg/d increased incident CVD (HR T3 vs. T1 = 1.47, 95% CI = 1.02–2.12). This association was potentiated (HR T3 vs. T1 = 2.42, 95% CI = 1.19–4.89) in subjects who had a lower intake of Ca (< 981 mg/d); in a low-Ca diet, an even lower amount of dietary Ox (second tertile, 148–220 mg/d) was related to increased CVD events by 92% (HR = 1.92, 95% CI = 1.00–3.70). No association was observed between dietary Ox and CVD events in the presence of medium- and high levels of Ca intakes. The critical cut-off point of Ox-to-Ca for predicting CVD events was 0.14, which was related to an increased risk of CVD by 37% (HR = 1.37, 95% CI = 1.02–1.84).ConclusionHigher dietary Ox intake appeared to be associated with a modestly elevated risk of incident CVD, especially in a diet with a lower amount of Ca.

CORRELATION BETWEEN DIETARY HABBITS WITH SEVERITY OF DYSMENORRHEA AMONG ALDOLESCENT GIRL

Abstract Introduction: More than 50% of women in each country on the world have dysmenorrhea. Dysmenorrhea can disturbing daily activities ,reduces the quality of life and learning activity indeed absent from school for a few hours or days. The purpose of this study is to determine corelation between diet and the severity of dysmenorrhea in SMK Negeri 2 Lumajang. Method: This study used observational analytic study design with a cross-sectional approach. The population was all female student in SMK Negeri 2 Lumajang. Total sample are 89 students calculated with Slovin formula. Used questionnaire as a instrument. Analysis of this study using chi square test. Result: Most of the respondents had good fat intake, while intake of Ca and Fe was less, frequency of eating 2x/day, total of bad intakes was 2, frequency of eating 2x/day, and severity of dysmenorrhea is severe dysmenorrhea . results of the chi square analysis of fat towards severity of dysmenorrhoea got p = 0.001, on fe, ca and the amount of intake that did not match towards severity of dysmenorrhoea, p = 0.000 and on the frequency of dysmenorrhea towars severity of dysmenorrhea, p = 0.773. Conclusion : there is a corelation between dietary habbit according to intake of fat, Fe, Ca, total bad intake with the severity of dysmenorrhea. And there is no corelation between dietary habbit according to eating frequency with the severity of dysmenorrhea.