The current study aimed to develop a herbal hair cream formulation including plant extracts traditionally used for hair growth, and further research was conducted to understand the hair growth capability better using the 5 alpha reductase inhibitory model. 5 alpha reductases, an enzyme confined to the nuclear membrane, converts testosterone into dihydrotestosterone. The 5 alpha-reductase and its metabolite dihydrotestosterone influence various human disorders, including male pattern baldness, benign prostatic hyperplasia (BPH), and prostate cancer. Finasteride and alfatradiol are examples of synthetic medications; however, because of their negative impacts, researchers are currently focused on creating safer 5α-reductase inhibitors from plants. The hair cream formulation contained 0.5% extracts of Hibiscus rosa sinensis flower, Eclipta alba whole plant, and Solanum nigrum plant berries in a ratio of 1:1:1. Plant extracts used in the formulation were characterized by chemical biomarkers. The cream formulation was developed, and the quality control parameters were examined. Furthermore, the produced formulation's 5α-reductase inhibitory activity was tested using finasteride as a positive control. H. rosa sinensis, E. alba, and S. nigrum extracts, which comprise 0.35% oleanolic acid, 4.65% β-sitosterol, and 31.18% linoleic acid, were used in the formulation. HPTLC data revealed that each 10 gm of cream formulation contains 16.68 ± 0.21 mg of linoleic acid, 3.61 ± 0.18 mg of β-sitosterol and 0.380 ± 0.11 mg oleanolic acid. In-vitro results showed a good 5α-reductase inhibitory potential. The IC50 value of the developed formulation was 85.254 ± 0.888 μg/ml, whereas finasteride (positive control) was 223.039 ± 1.367 ng/ml. Thus, the herbal hair cream composition benefits alopecia therapy by inhibiting the 5α- reductase enzyme. In addition, the formulation might be a potential option for further research into its antiandrogenic properties.