Ingredient Of Volatile Oil Research Articles

Chemical Composition and Phytotoxic Activity of Mentha vagans Boriss. Essential Oil.

This study explored the composition of essential oil (EO) and the first phytotoxic screening of EO obtained from the stems and leaves of Mentha vagans Boriss (MVEO) via hydro-distillation technique. The EO ingredients were detected through Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS analysis revealed that MVEO contained 49 constituents, constituting 93.95 % of the total oil. Among MVEO constituents, dihydrocarvone was observed as the dominant constituent (24.14 %), followed by D-carvone (16.28 %) and piperitone (18.14 %). The phytotoxic effects of MVEO and its dominant compounds were examined against Amaranthus retroflexus, Lolium perenne, and Poa annua. Significant inhibition was observed by MVEO in comparison with the major constituents and their mixture, suppressing the seedling growth of tested species at the lowest dosage (0.01 mg/mL); in general, seedling growth of all tested species was markedly inhibited when applied concentration of the EO and its constituents reached 0.05 mg/mL. Our results also indicated that constituents other than the dominant compounds of MVEO possessed considerable phytotoxic effects because the EO's activity was stronger than its major constituents and their mixture. Thus, additional studies are required to investigate MVEO and its constituents and commercialize them as environment-friendly bio-herbicides.

Exploring the aroma profile and biomedical applications of Scutellaria nuristanica Rech. F.: A new insight as a natural remedy

BackgroundThe Scutellaria genus has promising therapeutic capabilities as an aromatherapy. Based on that and local practices of S. nuristanica Rech. F. The essential oil was studied for the first time for its diverse biomedical applications. PurposeThis study aimed to evaluate and validate their therapeutic capabilities by screening the essential oil ingredients and examining their antimicrobial, antioxidant, carbonic anhydrase, and antidiabetic using further In silico assessment and In vivo anti-inflammatory and analgesic capabilities to devise novel sources as natural remedies alternative to the synthetic drugs. MethodsEssential oil was obtained through hydrodistillation, and the constituents were profiled using GC–MS. The antimicrobial assessment was conducted using an agar well diffusion assay. Free radical scavenging capabilities were determined by employing DPPH and ABTS assay. The carbonic anhydrase-II was examined using colorimetric assay, while the antidiabetic significance was performed using α-Glucosidase assay. The anti-inflammatory significance was examined through carrageenan-induced paw edema, and the analgesic features of the essential oil were determined using an acetic acid-induced writhing assay. ResultsFifty constituents were detected in S. nuristanica essential oil (SNEO), contributing 95.93 % of the total EO, with the predominant constituents being 24-norursa-3,12-diene (10.12 %), 3-oxomanoyl oxide (9.94 %), methyl 7-abieten-18-oate (8.85 %). SNEO presented significance resistance against the Gram-positive bacterial strains (GPBSs), Bacillus atrophaeus and Bacillus subtilis, as compared to the Salmonella typhi and Klebsiella pneumoniae, Gram-negative bacterial strains (GNBSs) as well as two fungal strains Aspergillus parasiticus and Aspergillus niger associated with their respective standards. Considerable free radical scavenging capacity was observed in DPPH compared to the ABTS assay when correlated with ascorbic acid. In addition, when equated with their standards, SNEO offered considerable in vitro carbonic anhydrase II and antidiabetic capabilities. Additionally, the antidiabetic behavior of the 9 dominant compounds of SNEO was tested via In silico techniques, such as molecular docking, which assisted in the assessment of the significance of binding contacts of protein with each chemical compound and pharmacokinetic evaluations to examine the drug-like characteristics. Molecular dynamic simulations at 100 ns and binding free energy evaluations such as PBSA and GBSA models explain the molecular mechanics and stability of molecular complexes. It was also observed that SNEO depicted substantial anti-inflammatory and analgesic capabilities. ConclusionHence, it was concluded that the SNEO comprises bioactive ingredients with biomedical significance, such as anti-microbial, antioxidant, CA-II, antidiabetic, anti-inflammatory, and analgesic agents. The computational validation also depicted that SNEO could be a potent source for the discovery of anti-diabetic drugs.

Chemical composition and bioactivity variability of two-step extracts derived from traditional and “QiNan” agarwood (Aquilaria spp.)

One of the primary applications for agarwood lies in the extracts, instead, there are obvious differences in the demands for agarwood components with different application fields. To obtain the rough separation and clarify each part's activity, four extracts of essential oil, hydrolat, extractum, and ethanol precipitation from traditional agarwood (TraA) and “Qinan” agarwood (QinA) were obtained by steam-solvent multistage extraction and ethanol precipitation. We investigated the chemistry and biological activity of multistage extracts using gas chromatography–mass spectrometry (GC–MS), high-performance liquid chromatography (HPLC), and in vitro activity testing. The results demonstrated that two kinds of agarwood essential oils contained mainly sesquiterpenoids, yet the sesquiterpene species were remarkably diverse in two kinds of agarwood essential oils. Then, the TraA and QinA hydrolat, all predominantly aromatic and sesquiterpene, but with differences from the essential oil ingredients. Additionally, the extractum chiefly contained chromones and the ethanol precipitation method worked well to separate the impurities in the TraA extract, however, it was ineffective for the QinA extract. Ultimately, essential oils and extractums all have antioxidant properties, with extractums outperforming essential oils. Moreover, both extractums and essential oils exhibited excellent broad-spectrum antimicrobial activity and anti-inflammatory activity. The findings pointed to the feasibility of separating the primary components from TraA and QinA using a multi-stage extraction technique, providing a scientific basis for the efficient utilization of all components of agarwood, as well as the functional product development and differentiated utilization of extract products in incense, fragrance, perfume, and daily chemicals.

The performance of plant essential oils against lactic acid bacteria and adverse microorganisms in silage production.

Plant essential oils have played an important role in the field of antibiotic alternatives because of their efficient bacteriostatic and fungistatic activity. As plant essential oils are widely used, their activity to improve the quality of plant silage has also been explored. This review expounds on the active ingredients of essential oils, their bacteriostatic and fungistatic activity, and mechanisms, as well as discusses the application of plant essential oils in plant silage fermentation, to provide a reference for the development and application of plant essential oils as silage additives in plant silage fermentation feed.

Chemical constituents from Eupatorium fortunei and their anti-inflammatory evaluation by in silico and experimental approaches

The well-known aromatic and medicinal plant Eupatorium fortunei Turcz. is widely cultivated in China, and previous studies on its bioactive constituents mainly focus on the essential oil ingredients especially thymol derivatives. However, reports on other type of constituents and the potential application are lacking. In the present project, an intensive chemical fractionation on the aerial part extract of E. fortunei led to the isolation and identification of a series of fatty acid derivatives (lipids, 1a/1b-19) including seven pairs of previously undescribed enantiomers (1a/1b-7a/7b), as well as a lignan (brachangobinan A (BBA), 20) and two monoterpenes (8S/8R-9-hydroxythymol, 21a/21b). A preliminary biological evaluation of these compounds in a NO production inhibitory assay model demonstrated compound BBA as the most active one. Network pharmacology analysis was used to predict and explore the possible anti-inflammatory targets and mechanism of BBA, which revealed some potential inflammation-related proteins and signaling pathways. Further experimental investigations validated that the anti-inflammatory effect of BBA could be achieved by suppressing pro-inflammatory factors and blocking the activation of NF-κB signaling pathway. Taken together, our work shows that E. fortunei can serve as a potential resource of lipids and anti-inflammatory agents.

Efficacy of <em>Eucalyptus citriodora</em> and <em>Syzygium aromaticum</em> essential oil as insecticidal, antiovipositant, and fumigant against <em>Callosobruchus maculatus</em> F (Coleoptera: Bruchidae)

Callosobruchus maculatus is the most pernicious pest of stored grain worldwide. Even though synthetic insecticides are commonly used to eliminate this insect pest, the negative effect of this pest management method on humans and the environment raises concern among people around the world. This study was done to identify the active ingredient of essential oils in Eucalyptus citriodora and Syzygium aromaticum and to evaluate the effectiveness of those essential oils in controlling C. maculatus. The results of gas chromatography/mass spectrometry analysis indicated that the essential oil extracted from the leaves of S. aromaticum are rich in Eugenol and β caryophyllene as much as 81% and 14.65% consecutively, while E. citriodora oil contains 86% of Citronella. According to the bioassay results, increasing the essential oil concentration from 1% to 3% resulted in a significant increase in insect mortality rate, oviposition deterrence, and fumigant toxicity. Additionally, S. aromaticum has significantly shown a higher insecticidal performance compared to E. citriodora. However, there are no synergistic effects observed on the use of essential oil of both plant species on C. maculatus. These results suggest that S. aromaticum and E. citriodora essential oils could be potential candidates as a natural insecticide in managing C. maculatus in stored products.

The Effect of Different Concentrations of Rice Washing Water as a Growth Regulator on the Quality of Cuttings of Jasmine Plants

Jasmine plant is a flowering plant that has many benefits. One of them is the basic ingredient of essential oils. This study aims to determine the effect of soaking ZPT in rice washing water and the dosage of rice washing water on the quality of jasmine cuttings. This study used a one-factor Completely Randomized Design (CRD) method with six concentrations of rice washing water, namely: P1 (ZPT Root Up 5%), P2 (Rice Washing Water 50%), P3 (Fermentation Rice Washing Water 40%), P4 (45% Rice Wash Water Fermentation), P5 (50% Rice Wash Water Fermentation), P6 (55% Rice Wash Water Fermentation), with 4 replicates obtained 24 experimental units. The parameters observed were the time of emergence of shoots, the percentage of the number of shoots, shoot length, number of roots and root length. The results obtained showed that the best concentration of immersion using rice washing water on the quality of jasmine cuttings was the fermentation treatment of rice washing water 40% and 45%.

Food Allergens and Essential Oils in Moisturizers Marketed for Children in Japan.

Introduction Personal skincare leave-on products increase the risk of food allergies. Parents must be imparted with an elevated degree of cognizance regarding the allergenic nature of pediatric skincare products. Material and methods We aimed to examine the data inferred from the promotional material on labeling these products about their proclivity to elicit skin sensitization. This study investigated the relationship between food allergens and essential oil ingredients and highlighted marketing terms, product prices, and ratings of moisturizers for children that are sold on Amazon, Japan. We searched and recorded the product labels and website marketing terms, price (per gram or milliliter), the number of reviews, and allergens and investigated the relationship between the percentage of food allergens in those products and marketing terms, price, and the number of Amazon reviews. Results Among the 164 pediatric skincare products we included, 144 (87.8%) that were manufactured in Japan were the most common; 7 (4.3%), 15 (9.1%), 23 (14.0%), 24 (14.6%), and 54 (32.9%) contained the eight regulated food allergens, grain, nut, fruit, and essential oils, respectively. Marketing terms emphasizing "natural/organic" were more likely to contain grain allergens and essential oils and were more expensive with and without "organic" labeling, respectively, whereas those labeled with marketing terms emphasizing "hypoallergenic" were less likely to contain fruit allergens or essential oils. Products with fewer Amazon reviews were more likely to use the marketing term "natural/organic" and had a higher grain allergen content. Conclusion In Japan, 4.3% of children's skincare products sold on Amazon contain eight food allergens that should obligatorily be labeled when included in food products. In addition, more than 10% of these children's skin care products contain ingredients derived from nuts, while more than 30% contain fruit extracts or essential oils.

Sodium Nitroprusside-Induced Changes in Physiological Traits and Essential Oil Ingredients in Vetiver Grass (Chrysopogon zizanioides (L.) Roberty) Under Water Deficit Stress

Sodium Nitroprusside-Induced Changes in Physiological Traits and Essential Oil Ingredients in Vetiver Grass (Chrysopogon zizanioides (L.) Roberty) Under Water Deficit Stress

POTENSI MINYAK KAYU PUTIH (Melaleuca cajuputi) SEBAGAI SUMBER BAHAN AKTIF DALAM PEMBUATAN HAND SANITIZER LIDAH BUAYA

Hand sanitizer as a medium to maintain hand hygiene is becoming a trend among the community today, especially because of the COVID-19 pandemic. Hand sanitizers with active ingredients of essential oils are considered safer than alcohol. The purpose of this research was to examine the addition of cajuput oil in the manufacture of aloe vera hand sanitizer on the physical quality, antiseptic and organoleptic activity of the hand sanitizer produced. The research design used a completely randomized design (CRD) with one factor, namely the addition of cajuput oil, with five treatment levels, namely 0 mL, 16 mL, 20 mL, 24 mL, and 28 mL with each treatment in three replications. The hand sanitizer produced was then tested for physical characteristics (density, spreadability, pH, and homogeneity), antiseptic activity, and organoleptic characteristics (color, texture, aroma and non-sticky impression). The results showed that the aloe vera hand sanitizer sample with the addition of 28 mL cajuput oil had a stronger antiseptic effectiveness among other samples and had physical characteristics that met the quality requirements of SNI hand sanitizer including pH 7.33, spreadability 6.96 cm, density 1.1546, homogeneous without any coarse grains and organoleptically acceptable and favored by the panelists.

A novel screening method for ingredients of essential oil to identify antibacterial compounds based on growth curve shape

We established a novel screening method to identify antibacterial compounds and used the method to screen various triterpenoids, which are difficult to assay using existing methods. The method involves comparison of the shape of bacterial growth curves based on changes in culture turbidity. Growth curves were grouped using multiple classification analysis. The antibacterial compounds tested belonged to a different chemical group than the controls. Six and four of the triterpenoids screened using the method suppressed the growth of Staphylococcus aureus FDA209P and Escherichia coli. W3110, respectively. Two triterpenoids exhibited bactericidal activity against S. aureus, and the others exhibited bacteriostatic activity.

Effect of Local Anesthetics on Dipole Potential of Different Phase Membranes: A Fluorescence Study.

The molecular mechanism behind the action of local anesthetics is not well understood. Phenylethanol (PEtOH) is an ingredient of essential oils with a rose-like odor, and it has previously been used as a local anesthetic. In this work, we explored the effect of PEtOH on dipole potential in membranes representing biologically relevant phases, employing the dual-wavelength ratiometric method utilizing the potential-sensitive probe di-8-ANEPPS. Our results show that PEtOH reduces membrane dipole potential in membranes of all biologically relevant phases (gel, liquid-ordered, and fluid) in a concentration-dependent manner. To the best of our knowledge, these results constitute one of the early reports describing reduction of membrane dipole potential induced by local anesthetics, irrespective of membrane phase.

Molecular Dynamics Simulations of Essential Oil Ingredients Associated with Hyperbranched Polymer Drug Carriers.

Our work concerns the study of four candidate drug compounds of the terpenoid family, found as essential oil ingredients in species of the Greek endemic flora, namely carvacrol, p-cymene, γ-terpinene, and thymol, via the simulation method of molecular dynamics. Aquatic solutions of each compound, as well as a solution of all four together in realistic (experimental) proportions, are simulated at atmospheric pressure and 37 °C using an OPLS force field combined with TIP3P water. As verified, all four compounds exhibit a strong tendency to phase-separate, thereby calling for the use of carrier molecules as aids for the drug to circulate in the blood and enter the cells. Systems of two such carrier molecules, the hyperbranched poly(ethylene imine) (HBPEI) polyelectrolyte and hyperbranched polyglycerol (HPG), are examined in mixtures with carvacrol, the most abundant among the four compounds, at a range of concentrations, as well as with all four compounds present in natural proportions. Although a tendency of the terpenoids to cluster separately persists at high concentrations, promising association effects are observed for all drug–polymer ratios. HBPEI systems tend to form diffuse structures comprising small mixed clusters as well as freely floating polymer and essential oil molecules, a finding attributed to the polymer–polymer electrostatic repulsions, which here are only partially screened by the counterions. On the other hand, the electrically neutral HPG molecules cluster together with essential oil species to form a single nanodroplet. Currently, terpenoid–polymer clusters near lipid bilayer membranes are being studied to determine the propensity of the formed complexes to enter cell membranes.

Structural and Functional Characterization of Nano Formulated Lemon Oil and Aloe Vera Based Essential Oil Ingredients By DLS, SEM and FTIR for Biomolecular Interactions

Aim:The major aim of this study is to identify the biomolecular interactions of nanoformulated essential oils. Materials and methods: Lemon oil and aloe vera nanoemulsion (N=12) preparation was done by standard protocol. It is further used for Particle size analysis in nanometre range, average mean diameter of particle size distribution and functional groups using sophisticated instruments like DLS, SEM and FTIR. Results:The results of this study has shown that average particle size of lemon oil and aloe vera nanoemulsion was obtained by DLS was 336±1nm, the mean diameter of the particle size was obtained by SEM was 42±2nm and in FTIR various functional groups were identified.Conclusion:The particle size analysis, average mean diameter of particle size and functional groups of nanoformulated lemon oil and aloe vera shows higher biomolecular interactions than the essential oils

Spectrum-Efficacy Relationships between GC-MS Fingerprints of Essential Oil from Valerianae Jatamansi Rhizoma et Radix and the Efficacy of Inhibiting Microglial Activation

The bioactive ingredients of essential oil from Valerianae Jatamansi Rhizoma et Radix (the Rhizome et Radix from Valerianae Jatamansi Jones) (EOVJRR) on the efficacy of inhibiting microglial activation were investigated with the approach of spectrum-efficacy relationship. Fourteen batches of Valerianae Jatamansi Rhizoma et Radix were extracted and analyzed by gas chromatography-mass spectrometry (GC-MS), and their activities in the efficacy of inhibiting microglial activation were assayed by measuring the inflammatory responses induced by lipopolysaccharide (LPS) in microglia cells from mice. The spectrum-efficacy relationships between fingerprints and the efficacy of inhibiting microglial activation of EOVJRR were established by grey relational analysis (GRA). Twenty common peaks were obtained from the GC-MS fingerprints of EOVJRR. P12 (vetivenol), P1 (bornyl acetate), P5 (seychellene), and P3 (β-elemene) indicated inhibition on microglia activation together, according to the spectrum-efficacy relationships. The current results established a general model for the spectrum-efficacy relationships of EOVJRR by GC-MS and the efficacy of inhibiting microglial activation, which could be applied to identify the bioactive ingredient and control the quality of herbs.

Differential Proliferative and Cytotoxic Effect of Selected Components of Essential Oils on Human Glioblastoma Cells

Background: In the study of bioactive agents from traditional medicine, mono- and sesquiterpenes represent the main ingredients of essential oils. Till now, only thymoquinone and perillyl alcohol have been clinically tested on glioblastoma. Objective: In the present study, we examined the effect of ten different essential oils on three human glioblastoma cell lines and one healthy human cell line. Methods: We used confocal laser scanning microscopy, flow cytometry, and cell growth analysis to evaluate cell morphology changes, membrane disruption effects, acute cytotoxicity and effects on the proliferation rate caused by the essential oils pinene, geraniol, eucalyptol, perillaldehyde, limonene, and linalool, perillyl alcohol, myrcene, bisabolol and valencene on human cells. Caspase 3/7 activity was measured to observe apoptosis induced by the essential oils. Results: We found that the cytotoxicity concentrations varied not only between different essential oils but also among different cell lines. Acute cytotoxicity of essential oils was based on cell membrane disruption and that HEK cells were affected to a much higher degree than the Glioblastoma cells. Vacuoles found in surviving glioblastoma cells appeared to be a factor in this effect. Conclusion: Caspase activity did not correlate with the membrane damage observed in the flow cytometry experiments. This is especially evident in the HEK cells that only showed apoptosis with two out of ten essential oils. Comparison of the effects of other essential oil to perillyl alcohol, which is already used in glioblastoma therapy, revealed perillaldehyde and valencene as two new candidate substances that showed even stronger anti-glioblastoma effects in all experiments.

Pomelo (<i>Citrus grandis</i> L.) Essential Oil Extraction: A Comparison between Hydrodistillation and Microwave Assisted Hydrodistillation

Hydrodistillation (HD) is a traditional technique used in most extraction processes. On the other hand, microwave-assisted hydrogen distillation (MAHD), an advanced method using microwaves in the extraction process, has recently emerged. The chemical ingredients of essential oils obtained from pomelo (Citrus grandis L.) peels obtained by MAHD and by hydrodistillation (HD) were analyzed and compared gas chromatography-mass spectrometry (GC/MS). The results show that there is no significant difference between the two methods in terms of extraction efficiency, at around 4.45 to 4.7%. The main components of essential oils were Limonene, α-pinene, β-Myrcene and Sabinene. The content of those compounds showed no clear quantitative and qualitative difference between HD and MAHD. Experimental results show that the MAHD method provides a good alternative to extracting essential oils from grapefruit, saving time, operating costs and achieving more optimal levels. Keywords: Comparison, Pomelo (Citrus grandis L.), Essential oil, Hydrodistillation, Microwave assist hydrodistillation,

Pharmacognostic Evaluation of Trachyspermum Ammi (Ajwain Seeds) Seed Extract for Vulvovaginal Candidiasis (VVC)

Introduction: In recent years, people are more health-conscious and avoiding the use of synthetic drugs, which are not only harmful to them but also to the environment as they are manmade. So, there is an urgent need for naturally available biologically active compounds which are environment friendly and has tremendous health benefits for the welfare of human being. Trachyspermum Ammi, commonly known as ‘Ajwain,’ is a plant native to India. It has been shown to have antifungal, antioxidant, antimicrobial, antinociceptive, cytotoxic, hypolipidemic, antihypertensive, antispasmodic, broncho-dilating, antilithiasis, diuretic, abortifacient, antitussive, nematicidal, Anthelmintic and antifilarial properties. Furthermore, studies reveal the presence of a variety of phytochemical constituents, including carbohydrates, glycosides, saponins, phenolic compounds, volatile oil (thymol, -terpinene, para-cymene, and- and -pinene), protein, fat, fibre, and mineral matter containing calcium, phosphorous, iron, and nicotinic acid. Methods: TLC of essential oil of Trachyspermum ammi, was performed and revealed the presence of. Thymol. GC-FID & GC-MS analysis of essential oil shows the presence of 31 compounds. The most existing major compounds in essential oil were Thymol (33.40 %), γ-Terpinene (16.58 %), β-Pinene (13.82 %), 2-Pinene (6.02 %), 3-Cyclohexene-1-carboxaldehyde, 1,3,4-tri-methyl (5.35 %), p-Cymene (3.85 %), Linalool (2.89 %) and α-Thujene (2.44 %). Results: Antimicrobial activity of essential oil showed against Staphylococcus aureus and E. coli, it was proven by using cup well method by observing zone of inhibition to the microbial strains at different concentrations of essential oil. The minimum inhibitory concentration was also observed. Conclusion: It was confirmed that essential oil shows the antimicrobial activity as well as shows effect to fungal strains. In conclusion, the presence of numerous essential oil ingredients, and the results of experimental studies showed that Trachyspermum ammi essential oil possessed antioxidant, antibacterial, and antifungal action. Vulvovaginal candidiasis can be treated with this essential oil.

Phytochemistry and Bioactivity of Nepeta racemosa Lam.

Background and objectives: The genus Nepeta is used in traditional medicine for the antiseptic and astringent properties in cutaneous eruptions and snake bites. Nepeta racemosa was investigated here due to the rich source of phytochemical compounds in the Nepeta genus and the lack of any phytochemical studies. Methods: The aerial parts were extracted successively using n-hexane, chloroform, and methanol (MeOH) by maceration method, respectively. The MeOH extract was exposed to C18 Sep-Pak fractionation by a step gradient of MeOH-H2O. Further purification of the fractions by preparative reversed-phase HPLC yielded three compounds. The chemical ingredients of essential oil were determined by GC-MS. Free-radical scavenging activity of the extracts, fractions, as well as their total flavonoid and phenolics contents, were assessed using the DPPH method, AlCl3, and Folin-Ciocalteu reagents, respectively. Results: Phytochemical study of 20% and 40% solid phase extraction fractions of MeOH extract yielded one iridoid (8,9-epi-7-deoxy-loganic acid) and two phenylethanoids (forsythoside B and verbascoside), correspondingly. The 4aα,7α,7aα-nepetalactone (31.70 %), germacrene D (7.39 %), n-hexadecanoic acid (6.47 %), were the main compounds of essentials oil. The MeOH extract demonstrated high activity in terms of antioxidant activity, total phenolics content along with total flavonoids content (0.09 ± 0.01 mg/mL, 1581.80 ± 10.28 mg/100g and 33.01 ± 0.02 mg/100g) as well as 40% SPE fraction (0.01 ± 0.00 mg/mL, 659.20 ± 40.32 mg/100g and 22.5 ± 0.37 mg/100g), respectively. Conclusion: The presence of phenylethanoid derivatives as phenolic compounds appears to be an important antioxidant compound in Nepeta racemosa.

Potential of essential oil of galam (Melaleuca cajuputi) leaf waste in Palangka Raya City

One of the causes of environmental and climate problems is human-generated waste such as Galam (Melaleuca cajuputi) leaf waste. Galam leaf waste with Essential oil ingredients can be a solution to the problem. The research objective was to utilize Galam Leaf waste in the form of essential ingredients and to determine the impact of climate change from the continuous utilization of Galam Plants The formula used was 10 liters of water, 4, 5, and 6 kg of Galam leaf biomass. Distillation activities carried out for 3 hours/formula. Repetition of each formula is done 5 times with the distillation technique. The essential oil results obtained from the formula of 4 kg of Galam leaf biomass are 4 ml, 6.2 ml, 12 ml, 7.5 ml, and 14 ml. Formula 2 is 11.5 ml, 16 ml, 14.5 ml, 13.5 ml and 15.5 ml. Formula 3 is 24.5 ml, 28 ml, 27 ml, 20.5 ml and 16 ml. Galam leaf waste processing activities can be a solution to reduce the impact of environmental and climate pollution.