Fruit flies, especially the most common species, Drosophila melanogaster, continue to be a useful organism for genetic research and for demonstrating principles of genetics at high school, college and graduate study levels. A traditional method of culturing Drosophila is in halfor quarter-pint milk bottles to obtain large cultures and in shell vials or creamers for single pairs or small cultures. Culturing Drosophila in conventional glassware containers presents, to a certain degree, several unresolved problems. (1) Etherized ffies often adhere to the medium or become entrapped in moisture when starting a new culture. (2) Collection of adult flies from vials or bottles necessitates shaking the flies to the bottom of the container prior to removal of the plug or cover. Such a procedure often causes a large number of flies to stick to the medium, and others may escape during removal of the plug and alignment with the etherizing chamber. This problem is especially troublesome in student laboratories where inexperienced people are handling cultures. (3) In the process of transferring flies from containers to the etherizing chamber, the medium often becomes dislodged and falls into the etherizing chamber, killing many flies. (4) Furthermore, etherization delays the onset of egg-laying for a certain period of time depending upon the depth and duration of anaesthesia, which may be undesirable under certain circumstances. A major problem is the process of satisfactorily cleaning glassware from discarded cultures. Although labor saving washing devices may be employed, a cleaning operation remains a sizable and unpleasant chore. Arnold (1957) described a method of using disposable paper containers for culturing Drosophila which was a major step toward overcoming problems associated with bottle cultures. Briefly, he employed half-pint plastic-lined Nestyles made by the Sealright Co., Fulton, N.Y. He immobilized the flies within the container by a device consisting of a polyethylene catsup bottle containing a few facial tissues saturated with ether. A hypodermic needle fitted on the end was used to inject either vapors into the container while it was upside down, thus allowing the immobilized flies to fall on the cover. The medium was anchored by a gauze bandage that was glued to the bottom before the medium was poured. Both of us have independently developed efficient methods for culturing and handling flies. Moyer et al. (1961) described a better anchor for Arnold's system and an etherizing procedure which may be more efficient for a large-scale operation. Yarbrough and Thomas (1963) showed that immobilization of flies by carbon dioxide had no effect on daily egg production. We describe here a combination of our methods and further development of modifications.