The interconnectedness of the post-intensive care syndrome domains: A qualitative study.

Tension wood (TW), a type of reaction wood that develops in angiosperm trees in response to gravistimulation, serves as an ideal model for investigating the regulatory mechanisms underlying xylem cell differentiation and cell wall deposition. The initial biological signals that induce the formation of reaction wood in response to gravitational stimuli remain poorly understood. In this study, we utilized pharmacological and genetic approaches to modulate Ca2+ levels in hybrid white poplar (Populus alba × P. glandulosa) and examine the role of calcium signaling during the early stages of gravitropic responses. Our findings revealed differential cytosolic Ca2+ signal distribution in gravistimulated stems during the early phase of gravity induction, characterized by lower Ca2+ levels on the upper side (where TW forms) and higher Ca2+ levels on the lower side (where opposite wood forms). Consistent with this hypothesis, plants treated with LaCl3 and those with genetically disrupted calcium channels (PagGLR3.3 knockout using the CRISPR/Cas9 system) showed reduced Ca2+ signals and developed characteristic TW features. These results suggest that decreased Ca2+ levels induce the formation of TW. Furthermore, PagGLR3.3 knockout plants with TW-like stems displayed diminished sensitivity to gravistimulation. Transcriptomic analysis revealed that the knockout of PagGLR3.3 resulted in the upregulation of genes associated with TW formation and reactive oxygen species (ROS) production. Notably, superoxide anion (O2 ·-) levels were significantly elevated in the cambium zone of stems subjected to gravistimulation, LaCl3 treatment, or PagGLR3.3 knockout, indicating that reduced Ca2+ levels promote TW formation through increased O2 ·- accumulation. This study offers novel insights into the critical role of Ca2+ in gravitropism and TW induction in poplar.

Effects of rumination and distraction on working memory performance in individuals with depressive tendencies and trait anxiety.

Key hemodynamic parameters during induced hypothermia cooling phase in healthy and injured piglets.

Background: Fentanyl is a potent opioid analgesic commonly used in anesthesia for bariatric surgery due to its high efficacy. However, the appropriate fentanyl dosage for morbidly obese patients remains inconclusive. Objective: To compare the efficacy of two fentanyl dosages at 2 mcg/kg versus 1 mcg/kg administered during induction before intubation in morbidly obese patients undergoing laparoscopic sleeve gastrectomy. Materials and Methods: A prospective, randomized, single-blinded trial was conducted between November 2024 and July 2025. Adults, aged 18 years and older, with morbid obesity scheduled for laparoscopic sleeve gastrectomy under general anesthesia were randomized, one to one, to receive either 2 mcg/kg or 1 mcg/kg intravenous (IV) fentanyl during induction. Following intubation, all patients received 25 mcg IV fentanyl every 30 minutes. Outcomes included pain scores assessed by the visual analog scale (VAS) in the post-anesthesia care unit (PACU) and at 24 hours, intraoperative hemodynamic parameters, and postoperative blood sugar. Results: Forty participants were enrolled. VAS scores at PACU showed no difference between groups. At 24 hours, however, the median VAS was significantly higher in the 2 mcg/kg group compared with the 1 mcg/kg group at 28 (IQR 19.87 to 50) versus 16.2 (IQR 0 to 26.6) (p=0.0059). Intraoperative analysis revealed that the 2 mcg/kg group experienced significant reductions in heart rate and systolic blood pressure from baseline to one-hour post-intubation. Conversely, postoperative blood glucose increased significantly in the 1 mcg/kg group. No differences were observed in analgesic and morphine consumption or perioperative complications. Conclusion: A dose of 1 mcg/kg of fentanyl administered during the induction phase before intubation results in lower postoperative pain scores at 24 hours compared with 2 mcg/kg in patients with morbid obesity undergoing laparoscopic sleeve gastrectomy. Both fentanyl dosages effectively control intraoperative hemodynamic parameters.

We evaluated the effects of growth hormone (GH) pretreatment on oocyte retrieval and embryo quality in patients undergoing assisted reproductive technology (ART) for diminished ovarian reserve (DOR). In this prospective cohort study, conducted at Shahida Islam Medical Complex from October 2023 to June 2025, 2,000 women aged ≤40 years with DOR were recruited before starting ART. DOR diagnosis was based on the 2017 criteria and the 2011 Bologna criteria. Participants were nonrandomly allocated to four equal groups: G1 (1 month of GH before ovulation induction+standard ART), G2 (2 months of GH pretreatment+ART), G3 (GH supplementation during the ovulation induction phase+ART), and G4 (control; standard ART). Hormonal and metabolic profiling was performed before, during, and after GH treatment. Accordingly, secondary stratification was applied to enable personalized treatment. Based on early response to GH pretreatment, participants underwent dynamic regrouping into good and poor responders. Key outcomes included oocyte retrieval, embryo quality, and maternal-fetal outcomes. GH pretreatment improved ART results, with the G2 group achieving the best estradiol levels, follicular growth, and oocyte retrieval, albeit with increased insulin resistance (homeostatic model assessment of insulin resistance [HOMA-IR]). The G1 and G3 groups also outperformed controls (G4). GH dose and anti-Müllerian hormone (AMH) level strongly predicted successful oocyte retrieval. Testosterone was negatively associated with retrieval; interleukin 6 and dehydroepiandrosterone sulfate (DHEA-S) had weaker effects. G2 embryos exhibited the best development and blastocyst formation outcomes, with the most advanced development by day 5. A nomogram was developed to predict oocyte retrieval outcomes from GH dose, AMH, HOMA-IR, and testosterone. In patients with DOR, GH pretreatment improves treatment outcomes. Over 1 month of pretreatment can increase the oocytes retrieved but confers higher insulin resistance. Thus, when using GH for more than 4 weeks, clinicians should closely monitor HOMA-IR and perform continuous glucose monitoring; otherwise, 1-month GH pretreatment is preferred when initiating ART.

Adipogenic differentiation efficiency remains highly variable across laboratories and cellular models, underscoring a critical need for a robust and standardized protocol. Here, we describe an optimized and highly effective protocol for inducing adipogenesis in multiple models, including murine 3T3-L1 preadipocytes, stromal vascular fraction (SVF) from neonatal and adult mice, and human adipose-derived stem cells (hADSCs). Systematic optimization was performed on key parameters such as initial cell confluence, induction timing, inducer composition, and culture surface coating. We show that high cell density, rosiglitazone supplementation, and an extended primary induction phase combine to promote lipid accumulation. Notably, we introduce a crucial modification-prolonged low-dose insulin stimulation during the maintenance phase-that is essential for the efficient differentiation of adult SVF. Furthermore, when applied to hADSCs, the protocol consistently induced robust adipogenesis, confirming its cross-species applicability. Taken together, this comprehensive and reproducible protocol serves as a valuable tool for advancing in vitro adipogenesis research. Key features • Extend a robust, standardized adipogenic differentiation protocol from 3T3-L1 preadipocytes to clinically relevant models, including hADSCs and the heterogeneous SVF. • Identify key optimized parameters-cell density, induction timing, and inducer composition-enabling highly reproducible differentiation across species.

Laboratory diagnostic in multiple myeloma: Quantification of daratumumab blood levels using isoelectric focusing.

Background: Acute lymphoblastic leukemia (ALL) is a cancer characterized by the rapid and uncontrolled growth of immature lymphoid cells. Among its subtypes, B-lineage ALL in children represents the most frequently diagnosed malignancy in the pediatric population. Despite high survival rates, relapsed or refractory cases remain challenging. Minimal Residual Disease (MRD), detected by flow cytometry, is a strong prognostic marker that guides the intensity of post-induction therapy. Aim: This study aims to evaluate the correlation between post-induction MRD, measured by multicolor flow cytometry, and the initial clinical and laboratory parameters in pediatric patients with B-ALL. Methods: A retrospective cross-sectional study was conducted at the Children’s Specialty Hospital in Basrah, including 51 pediatric B-ALL patients (aged 1–14 years) treated between April 2023 and August 2024. All patients successfully completed the induction phase of chemotherapy, and their MRD status was subsequently evaluated. Results: MRD was negative in 60.78% and positive in 39.22% of patients. The majority of patients in both MRD groups were under 10 years old, with no significant age-related difference (P = 0.732). Patients with positive MRD had significantly higher mean white blood cell (WBC) counts (58.62 ± 86.9 vs. 11.44 ± 13.04) and blast cell percentages (87.1% ± 7.6% vs. 76.6% ± 16%) compared to those with negative MRD (P = 0.004 and 0.008, respectively). No significant associations were found between MRD and hemoglobin levels, platelet counts, or National Cancer Institute (NCI) risk classification. Conclusions: Flow cytometric MRD detection is a valuable prognostic tool in pediatric B-ALL. Higher WBC counts and blast percentages at presentation are associated with positive MRD, while MRD was not significantly linked to age, hemoglobin, platelet count, or risk stratification

This study evaluated the skin sensitization of the antimicrobial peptide sublancin to support its safety assessment for topical application. Sensitization was assessed using the guinea pig maximization test (GPMT), in which animals received sublancin (2 mg/kg), vehicle (negative control), or 2,4-dinitrochlorobenzene (positive control) during induction and challenge phases. Skin reactions (erythema and edema) were recorded after challenge. Irritation was evaluated in rabbits following single and repeated applications of sublancin to intact and abraded skin, with observations made at multiple time points. In the GPMT, no erythema or edema was observed in the sublancin-treated group or negative control group at 24, 48, and 72 h post-challenge, corresponding to a sensitization rate of 0%. All animals in the positive control group exhibited moderate to severe erythema and edema (sensitization rate 100%). In both single- and repeated-dose rabbit irritation tests, sublancin induced no erythema or edema on intact or abraded skin at any observation point, resulting in a total irritation score of 0. Furthermore, no significant differences in the daily weight gain were observed between any experimental group and the negative group (p > 0.05). In conclusion, under the conditions of this study, sublancin showed no skin sensitization potential in guinea pigs and no irritant effects in rabbits, supporting its local tolerance for topical veterinary use.

Objective.Prompt identification of haematomas is crucial for effective clinical treatment. Magnetic induction phase shift technology (MIPS), known for its portability, non-contact nature, and affordability, is limited by the weak signal induced by cerebral hemorrhage leading to poor sensitivity, which is urgent to be improved.Approach. Tracer of magnetic nanoparticles is introduced to produce robust induced magnetic field. A symmetrical gradiometer coil is used as the receiving coil to nullify the effect of primary magnetic field generated by the excitation coil, which is designed as a Helmholtz coil.Main results.In vitroexperiments showcase the remarkably improved sensitivity and stability of the detection system, with magnetic nanoparticles notably boosting the MIPS signal for hemorrhage. Moreover,in vivoexperiments employing a rabbit autologous blood cerebral hemorrhage model reveal that with a hemorrhage volume of 2 ml, the experimental group with employed magnetic nanoparticles increased the MIPS signal change by 23-fold compared to the control group without magnetic nanoparticles.Significance. The sensitivity of MIPS for hemorrhage detection is significantly improved compared to traditional method. The magnetic nanoparticle-enhanced MIPS detection technique holds promise as an optimal solution for real-time, non-invasive bedside monitoring for cerebral hemorrhage.

P1109 Impact of concomitant mesalazine administration on the efficacy and safety of advanced therapies in ulcerative colitis: a meta-analysis over the induction and maintenance phases

P1192 Systemic corticosteroid use in IBD patients receiving advanced therapies: real-world data from IBDREAM

P0740 Observation on the Efficacy and Safety of Upadacitinib in Ulcerative Colitis: A Real-World Single-Center Retrospective Study

DOP029 One-Year Effectiveness and Safety of Upadacitinib in 261 Patients With Crohn’s Disease: A Multicenter Real-World Cohort Study.

P0658 Combination of Fecal Microbiota Transplantation and Exclusion Diet for Induction and Maintenance in Mild-to-Moderate Crohn’s Disease: A Randomized Sham-Controlled Trial

DOP005 Mirikizumab treatment reduces Crohn’s disease-related surgery and hospitalization rates: analyses from VIVID-1

P0800 Real-world use of upadacitinib in Inflammatory Bowel Disease patients in Greece: Focus on adverse events

P1051 Real-World Evaluation of Crohn’s Disease Activity During Risankizumab Induction Using Ultrasound Assessment: Preliminary Results from the AN-IBD Network

Abstract The development of effective and attractive online fault detection techniques is crucial because three-phase induction motors (IMs) with lower power ratings are more vulnerable to inter-turn short-circuits (ITSC). Conventional induction motor protection techniques frequently fall short in identifying these issues, instead pointing to overload or phase imbalances and, in certain situations, sending out pointless trip commands that cause disruptions and expensive downtime. Early detection at low fault severity levels is essential because ITSC defects can cause serious harm if they go unnoticed. In order to detect ITSC in squirrel cage induction motors, a unique hybrid coherence estimator is presented in this study. By comparing faulty currents with baseline normal operating currents and analyzing the coherence between phase currents, the hybrid coherence estimator found notable deviations suggestive of inter-turn faults. According to statistical analysis, the algorithm produced no false positives and had a 100% fault detection accuracy under all loading scenarios. A 1 HP, 440V, 50 Hz, three-phase squirrel cage induction motor was used to test the suggested algorithm’s performance, taking into account inter-turn faults of one to ten turns. A range of loading circumstances, including 1.25 A, 1.35 A, 1.5 A, 1.6 A, and 1.7 A, were used to test the algorithm. The study was able to identify even the smallest flaws that can impair motor performance by using a controlled provision to short-circuit turns. The findings show that by enabling precise protective measures, this method not only extends the operational lifespan of squirrel cage induction motors but also increases their reliability through prompt problem identification. This cutting-edge approach provides a viable way to monitor and maintain squirrel cage induction motors in real-time, guaranteeing peak performance and minimal downtime.