Indonesian Mangroves Research Articles

Evaluation of phytochemical, antioxidant and anticancer activities of Indonesian mangroves herbal tea

Evaluation of phytochemical, antioxidant and anticancer activities of Indonesian mangroves herbal tea

Aboveground biomass and carbon stock estimation using UAV photogrammetry in Indonesian mangroves and other competing land uses

Mangrove ecosystem is one of coastal wetlands that have experienced significant anthropogenic driven degradation and conversion, which have resulted in substantial carbon releases to the atmosphere and the oceans. However, carbon stock assessments and monitoring for mangroves are mostly confined to in situ methodologies, which has limited further understanding of wider-scale spatial variations and additional driving factors of carbon loss. Here,we report a novel application using unmanned aerial vehicles (UAVs) to estimate and compare aboveground biomass (AGB) carbon stocks of mangrove forests with two alternate replacement commercial land uses (oil palm and coconut plantations) in Lubuk Kertang and Pulau Sembilan, North Sumatra, Indonesia. We generated high-accuracy digital surface models (DSMs) and digital terrain models (DTMs), as well as elevation data from a global navigation satellite system (GNSS). Through analysis, we highlight how mangrove forest conversion to oil palm and coconut plantations significantly decreases the aboveground biomass carbon (AGB) and carbon stocks (AGC) of selected mangrove ecosystems in Indonesia. In this study, spatially mapped biomass carbon measurements were found to be more accurate when cross-validated with in situ measurements. AGC stocks were found to be over 10- to 12-fold that of converted oil palm and coconut plantations across selected study sites. The generated biomass carbon stock product provides new knowledge of carbon stock distribution in Indonesia across mangrove forests and two alternative commercial land use types within similar topographical landscapes. This combined methodological approach may be a useful tool to assist with blue carbon inventories as well as for selecting specific mangrove conservation and restoration areas across larger spatial scales.

Carbon stocks and fluxes in Asia-Pacific mangroves: current knowledge and gaps

Mangrove forest plays a key role in regulating climate change, earth carbon cycling and other biogeochemical processes within blue carbon ecosystems. Therefore, mangrove forests should be incorporated into Earth system climate models with the aim of understanding future climate change. Despite multiple carbon stock and flux assessments taking place over the past couple of decades, concrete knowledge of carbon source/sink patterns is largely lacking, particularly in the biodiversity-rich Asia-Pacific (AP) region with its 68 493 km2 of mangrove area. Thus, to understand the gaps in mangrove blue carbon research in the AP region, we summarize a recent decade-long inventory of carbon stock pools (aboveground, belowground and soil) and biogeochemical flux components (burial, export/import, soil-air and water-air CO2 flux) across 25 AP countries to understand the current knowledge and gaps. While carbon stock assessments of individual components are available for all 25 countries, whole ecosystem carbon stocks—including live and standing dead aboveground and belowground, downed woody debris and soil carbon stocks—are often lacking, even in highly researched countries like Indonesia. There is restricted knowledge around biogeochemical carbon fluxes in 55% of the countries, suggesting poor carbon flux research across the region. Focusing on flux components, reports on sediment-to-sea carbon exports are extremely limited (coming from just nine countries in the AP region). There is notable scarcity of data on carbon export fluxes in Indonesian mangroves. Given the key role AP mangroves play in climate change mitigation worldwide, more detailed and methodologically comparable investigation of biogeochemical source/sink processes is required to better understand the role of this large carbon source in global carbon stocks and fluxes, and hence, global climate.

Diversity of Myxobacteria Isolated from Indonesian Mangroves and Their Potential for New Antimicrobial Sources

Mangroves are unique intertidal ecosystems that provide ecological niches to different microbes, which play various roles in nutrient recycling and diverse environmental activities. The association between myxobacteria and mangroves are hitherto poorly understood. The aim of our study was to evaluate the myxobacterial community composition as well as isolate myxobacteria and to characterize the antimicrobial activity of myxobacteria isolates from Indonesian mangroves. Twenty-five cultivable myxobacteria were affiliated in six genera: Myxococcus, Corallococcus, Archangium, Chondromyces, Racemicystis and Nannocystis of the order Myxococcales based on partial 16S rRNA gene sequences. Thirteen crude extracts showed moderate activities against at least one of human pathogenic microorganisms. The crude extract of Racemicystis sp. strain 503MSO indicated a novel compound, which has not been reported in the database yet and the identification of this compound needs further study. The myxobacterial communities of three different sampling sites were analyzed using primers adapted for the myxobacteria group identification. The results showed that myxobacterial communities are more diverse than assumed. Therefore, our study has highlighted the importance of the mangrove habitat as promising harbor of myxobacteria as well as novel antimicrobial compounds with activity against pathogenic microorganisms.

Exploration Of High Economic Value Microalgaes In The Mangrove Area Of Pari Island, Seribu Islands, Jakarta

Mangrove areas are widely reported as a place of abundance of microalgae producing omega-3 docosahexaenoic acid (DHA) and other bioactive materials of high economic value from the genus Aurantiochytrium. The search for microalgae was carried out in the mangrove area of Pari Island, Seribu Islands, Jakarta, in April 2022 and identified at the Biology Education Laboratory, FKIP UHAMKA. The purpose of this study is part of the long-term goal of collecting new strains or isolates from the genus Aurantiochytrium and other members of the family Thraustochytriidae from forests in Indonesian mangroves. This research was conducted by isolating microalgae with the pure streaking method and identifying them morphologically. This study succeeded in obtaining eight isolates (UHA1, UHA3, UHA5, UHA6, UHA10, UHA11, UHA12, and UHA13) which in the appearance of colonies and microscopic morphology lead to the species Aurantiochytrium sp. One of the isolates obtained (UHA12) was cultivated by batch culture for five days in an Erlenmeyer flask using glucose, yeasts extract, and seawater as a medium. The result is that the dry biomass obtained is almost equivalent to that of the Aurantiochytrium sp. other famous

Coastal protection system design at the Indonesian Mangrove Center in Pekalongan, Central Java – Indonesia

The Pekalongan area suffered from coastal flooding and heavy coastal erosion due to land subsidence and increasing wave condition in the last decade. Pekalongan government built a land dike in 2018 to protect Pekalongan city from coastal flooding. This study aims to determine a suitable coastal protection system, consisting of hard-structures and mangroves, to solve the erosion problem at the Pekalongan coast, specifically at the future Pekalongan Mangrove Information Centre (PIM). Environmental data, i.e., wind, wave, current, tidal, sediment, and soil data, were obtained by field survey and secondary data collection from open-source data. The selection of the most suitable coastal protection design concepts was performed by comparing several alternatives of structure materials. Coastal protection layout was obtained based on a numerical sediment transport model result using Delft3D software. From environmental data processing and analysis with various design aspects, the top elevation of the coastal protection structure is +4.5 m MSL (Mean Sea Level). The configuration of the mangrove planting media consists of ‘guludan’ structure technique with dimension 10 m x 5 m x 4 m.

Biodegraders of Large Woody Debris Across a Tidal Gradient in an Indonesian Mangrove Ecosystem

There has been limited research on the breakdown, recycling, and flux of carbon from large woody detritus (LWD) in mangrove forests. The breakdown of LWD is caused by guilds of terrestrial and marine biodegrading organisms that degrade wood at a range of rates and efficiencies. Spatial variations in environmental factors within mangroves affect the distribution and community of biodegrading organisms, which, in turn, impacts carbon flow and sequestration. We reveal the role of biodegrading organisms in LWD breakdown and the environmental factors that influence the distribution of biodegrading guilds within a mangrove forest in South East Sulawesi that supports a diversity of mangrove species typical of Indonesian mangrove forests, which constitute 20% of Global mangrove cover. Within the high intertidal regions, terrestrial biodegradation processes dominated upon LWD. After 12 months exposure on the forest floor, experimental wooden panels in these areas remained unchanged in mass and condition. In the low intertidal region, marine wood-boring animals belonging to the family Teredinidae were the dominant biodegraders of LWD, and their activity reduces LWD volume and speeds up the loss of LWD volume. More than 50% of the experimental wooden panels’ weight in these areas was lost after 12 months exposure on the forest floor. Although different biodegrading guilds occupy the same LWD niche, their distribution throughout the mangrove forest is influenced by inundation time. The change of biodegrading guilds within LWD between the terrestrial and the marine organisms was distinct, creating a biodegradation boundary in a distance as narrow as 1 m on the mangrove forest floor. These results are important, as rising sea levels have crucial implications for biodegrading guilds. A full understanding of factors affecting the biodegradation processes of LWD in mangrove forests is critical to accurately assess mangrove carbon stores and the fate of mangrove derived carbon.

Contributions of mangrove conservation and restoration to climate change mitigation in Indonesia.

Mangrove forests are important carbon sinks, and this is especially true for Indonesia where about 24% of the world's mangroves exist. Unfortunately, vast expanses of these mangroves have been deforested, degraded or converted to other uses resulting in significant greenhouse gas emissions. The objective of this study was to quantify the climate change mitigation potential of mangrove conservation and restoration in Indonesia. We calculated the emission factors from the dominant land uses in mangroves, determined mangrove deforestation rates and quantified the total emissions and the potential emission reductions that could be achieved from mangrove conservation and restoration. Based on our analysis of the carbon stocks and emissions from land use in mangroves we found: (1) Indonesia's mangrove ecosystem carbon stocks are among the highest of any tropical forest type; (2) mangrove deforestation results in greenhouse gas emissions that far exceed that of upland tropical deforestation; (3) in the last decade the rates of deforestation in Indonesian mangroves have remained high; and (4) conservation and restoration of mangroves promise to sequester significant quantities of carbon. While mangroves comprise only ≈2.6% of Indonesia's total forest area, their degradation and deforestation accounted for ≈10% of total greenhouse gas emissions arising from the forestry sector. The large source of greenhouse gas emissions from a relatively small proportion of the forest area underscores the value for inclusion of mangroves as a natural climate solution. Mangrove conservation is far more effective than mangrove restoration in carbon emissions reductions and an efficient pathway to achieve Indonesia's nationally determined contribution (NDC) targets. The potential emission reduction from halting deforestation of primary and secondary mangroves coupled with restoration activities could result in an emission reduction equivalent to 8% of Indonesia's 2030 NDC emission reduction targets from the forestry sector.

Taxonomy and Antimicrobial Activity of Gliding Bacterium from Indonesian Mangroves

The discovery of new antibiotic is needed, due to the increasing antimicrobial resistance in nature. Therefore, this study aims to isolate gliding bacteria and to ascertain their antimicrobial activity against pathogenic microbes. This research was conducted by isolating gliding bacteria from mangrove sediment in Muara Angke, North Jakarta, Indonesia. All of the strains were identified by 16S rRNA genes sequencing and those selected strain was characterized using polyphasic approach. The performance of crude extracts against to ten pathogenic microorganisms were detected using serial dilution test in 96-well plates. Mangrove gliding bacteria isolates designated 313MSO and 314MSO were showed high homology to Ohtaekwangia kribbensis with 96.20% and 99.12% similarity, respectively. The polyphasic approach led to the conclusion that the strain 313MSO was a new species of the genus Ohtaekwangia. Twenty-three crude extracts were obtained from cultivating the strain in twenty-three different media. The most of them inhibited the growth of Staphylococcus aureus Newman with the minimum concentration of 33.33-66.67 µg/ml. Four compounds (Marinoquinolines A-D) were obtained from HPLC-MS analysis. Furthermore, the strain 313MSO is presently being studied for in-depth identification of additional unknown metabolites detected in the crude extracts.

Review: Causes and impacts of anthropogenic activities on mangrove deforestation and degradation in Indonesia

Abstract. Cahyaningsih AP, Deanova AK, Pristiawati CM, Ulumuddin YI, Kusumawati L, Setyawan AD. 2022. Review: Causes and impacts of anthropogenic activities on mangrove deforestation and degradation in Indonesia. Intl J Bonorowo Wetlands 12: 12-22. Indonesia is a country with the largest extent of mangroves in the world. However, as the human population increases, the extent and quality of the mangroves decrease. The increasing population is in line with the increase in human activities, leading to deforestation and degradation of the mangrove forests. This paper aimed to review anthropogenic activities that cause mangrove deforestation and degradation in Indonesia and the impacts of such anthropogenic activities on the mangrove ecosystems and formulate the efforts to overcome mangrove deforestation and degradation in the country. Various human activities are the primary drivers of deforestation and degradation of mangroves, including land conversion from mangrove forests into other land uses (e.g., agricultural lands, ponds, infrastructure developments, and human settlements), extractive activities (e.g., mining and logging), and household and industrial activities (e.g., plastic debris, heavy metals pollutants). These anthropogenic factors reduce mangrove forest area, changes in mangrove composition, uneven species abundance, and pollution. Combined with sea-level rise caused by global climate changes, such activities pose devastating threats to Indonesian mangroves. Efforts to overcome these problems include increasing awareness of communities regarding mangrove conservation, conducting mangrove restoration programs, and promoting sustainable management of mangrove, for example, through ecotourism. On top of that, community participation plays an essential role in Indonesia's sustainable management and conservation of mangrove forests.

Bioprocess Potentials of Squalene from Thraustochytrids Microalgae for Nutraceuticals in New Normal Era Isolated from Indonesian Mangroves: A Review

The covid-19 pandemic has been increasing people's awareness of good eating habits and consumption to maintain their health. Accordingly, the choice shifted more to consume nutraceutical and functional food to provide a beneficial impact. One of the essential and attractive nutraceuticals is squalene (C 30 H 50 ). Squalene is a precursor for biosynthesis in all human steroids and has a long history as an essential compound in the pharmaceutical industry. A common source of this terpenoid comes from the liver oil of a deep-sea shark. However, the strategy for squalene production from liver sharks encountered environmental and political barriers due to strict nature protection regulations. The renewed scientific interest has found microalgae from the thraustochytrids family as a promising future source of squalene. Thraustochytrids is a group of Osmo-heterotrophic marine microalgae, which can be found commonly in mangrove are, has recently gained increased attention owing to its promising biotechnological potentials. As the country with the largest mangrove forest globally, Indonesia has the highest biodiversity potentials of Thraustochytrids. Unfortunately, the study on the potential of bioprocess technology using Thraustochytrids microalgae from Indonesian mangrove forests for squalene production has received less attention from researchers. Therefore, this paper presents Thraustochytrids' potential from Indonesian mangrove forests as a sustainable source of squalene production that can replace raw material from shark liver oil. This paper summarizes all selected strains used in the previous study and their operating parameters. Based on our review study, the most productive Thraustochytrid producing squalene comes from the Aurantiochytrium sp. 18W-13a–1, which operated in a temperature-controlled reciprocal 500 mL shaker of Sakaguchi flask (25 °C, 100 strokes min − 1,70 mm amplitude) and used nutrients of 2.0% glucose, 1.0% tryptone, 0.5 g /l yeast, and 50% artificial seawater (ASW). Compared to squalene from other sources (yeast, bacteria, and plants), Thraustochytrid can yield more squalene. Bioprocess engineering aspects and the general uses of squalene are also presented, including the notable developments in the adjuvant vaccine of Covid-19, anti-aging substance, and anti-cancer applications.

Analysis of Unusual Sulfated Constituents and Anti-infective Properties of Two Indonesian Mangroves, Lumnitzera littorea and Lumnitzera racemosa (Combretaceae)

Lumnitzera littorea and Lumnitzera racemosa are mangrove species distributed widely along the Indonesian coasts. Besides their ecological importance, both are of interest owing to their wealth of natural products, some of which constitute potential sources for medicinal applications. We aimed to discover and characterize new anti-infective compounds, based on population-level sampling of both species from across the Indonesian Archipelago. Root metabolites were investigated by TLC, hyphenated LC-MS/MS and isolation, the internal transcribed spacer (ITS) region of rDNA was used for genetic characterization. Phytochemical characterization of both species revealed an unusual diversity in sulfated constituents with 3,3’,4’-tri-O-methyl-ellagic acid 4-sulfate representing the major compound in most samples. None of these compounds was previously reported for mangroves. Chemophenetic comparison of L. racemosa populations from different localities provided evolutionary information, as supported by molecular phylogenetic evidence. Samples of both species from particular locations exhibited anti-bacterial potential (Southern Nias Island and East Java against Gram-negative bacteria, Halmahera and Ternate Island against Gram-positive bacteria). In conclusion, Lumnitzera roots from natural mangrove stands represent a promising source for sulfated ellagic acid derivatives and further sulfur containing plant metabolites with potential human health benefits.

Potential carbon storage of Indonesian mangroves

Even though it only covers 0.1% of the surface of the continent, mangrove forests recorded as carbon-rich forests on earth. Forest loss and degradation together, especially in the tropics contributes about 12% of the total annual anthropogenic CO2 emissions. Mangrove forests ecosystem have high carbon storage potential. The study results show that the ratio of carbon to area in mangrove ecosystems is almost 13 times greater than that of tropical rainforest ecosystems in Indonesia. This study has used published data recorded in Google Scholar and Science Direct. Indonesia’s mangroves, with a total area of 3.1 million ha, store a total carbon of 5.2 Gt. The average stand biomass or aboveground biomass is around 407.15 tons ha−1, storing carbon as much as 191.36 tons ha−1 which is equivalent to the potential for CO2 absorption (CO2e) by mangrove stands of 702.29 tons ha−1. Papua has the largest mangrove forest in Indonesia that has the potential for CO2e. Assessment of carbon storage in soil needs to be done in estimating the total carbon stored in mangrove ecosystems, given the large potential for stored carbon that plays a role in climate change mitigation.

Biological activities of Indonesian mangroves obtained by subcritical water extraction

Mangroves are still considered as underexploited marine resources in Indonesia, and functional materials from Indonesian mangroves are not characterized yet. In this study, different part of mangroves (Sonneratia alba leaves - SAL, Sonneratia alba roots - SAR, Sonneratia alba fruit - SAF, Rhizhopora mucronata - RMF, Rhizhopora lamarckii - RLF) were hydrolyzed using the subcritical water extraction (SCWE) system at 120 ºC. Mangroves hydrolysates (SAL, SAR, SAF, RMF and RLF) were further analyzed for total protein, sugar, reducing sugar, saponin and flavonoid content. The antioxidant capacity and functional material contents including total sugar, reducing sugar, protein, flavonoid and saponin contents. The highest flavonoid and saponin contents were obtained from RLF with the value of 20.13±0.17 mg/g and 31.83±0.18 mg/g, respectively. The antioxidant capacity and antibacterial properties of mangroves hydrolysates varied significantly based on the sample materials, with RLF showing the highest total antioxidant activity and antibacterial activity. The results of our study suggest that tropical mangroves especially R. lamarckii could be valorized efficiently, as a source of bioactive material using subcritical water extraction

Characteristics of Functional Materials Recovered from Indonesian Mangroves (Sonneratia alba and Rhizhophora mucronata) Using Subcritical Water Extraction

Mangrove is the most productive ecosystem in marine environment, as it provides a unique habitat opportunity for many marine species and key goods and services for human beings. In Indonesia, mangroves are still considered as underexploited marine resources, and functional materials from Indonesian mangroves are not characterized yet. In this study, two mangroves Sonneratia alba and Rhizhophora mucronata from Kendari, were hydrolyzed using the subcritical water extraction (SCWE) system. Three different temperature conditions (150–230 °C with 40 °C increments) were applied to obtain the best functional materials. Mangroves hydrolysate was further analyzed for total sugar, protein, phenol content. The antioxidant capacity and functional material contents varied significantly based on the sample materials and extraction conditions, with R. mucronata hydrolyzed at temperature 230 °C showing the highest antioxidant activity. High-performance liquid chromatography (HPLC) analyses revealed high contain of phenolic acids in all mangroves hydrolisates especially R. mucronata fruit (RMF). Collectively, this study shows the potential of mangroves resources in Indonesia in Indonesia for different applications (food, cosmetics and pharmacy) using environmental friendly technology.

A Historical Review of Data and Information of Indonesian Mangroves Area

Quantification of the mangrove area in Indonesia was very diverse, which is quantified in the pre and post development of remote sensing technology. This causes uncertainty comprehensive information of mangrove in Indonesia. The planners at national and international level of faced with the problems of uncertainty information that has implications for the quality of data availability. Therefore, it is important to develop a comprehensive of mangrove extent and distribution database. The method used in-depth study literature on various publications related mangrove mapping in Indonesia and comparative analysis. This study aims to identify comprehensive information mangrove forests of Indonesia and analyze the factors which cause diversity of quantification area and reliability of data. We found 42 publications containing data and information on Indonesia's mangrove forests area, with 17 sources including detailed information per province. The study results showed that the difference mangrove quantification area was influenced by several main factors including analysis periode, differences in the concept and mangrove boundaries, basic data and scale, method of mangrove mapping, as well as regional coverage. Keywords: historical data, Indonesia, mangrove area

Complete Genome Sequence of the Putative Phosphonate Producer Streptomyces sp. Strain I6, Isolated from Indonesian Mangrove Sediment.

Streptomyces sp. strain I6 is a novel strain isolated from an Indonesian mangrove sediment sample. Bioinformatic analysis of the genome sequence of Streptomyces sp. I6 revealed 23 biosynthetic gene clusters. One of them encodes the synthesis of a putative phosphonate secondary metabolite, a class of underexplored natural compounds with great pharmaceutical potential.

The Production of Activated Carbon from Indonesian Mangrove Charcoal

Activated Carbon is an important material for purification, colour removal, adsorbent, and catalyst in the chemical industry. One of the iodine industries in East Java use active carbon as adsorbent that captures iodine from the solution. Indonesia still imports activated carbon despite the numerous raw materials of activated carbon in Indonesia. This research aims to determine the effect of the concentration of phosphoric acid (activating agent) and activation temperature on the production of activated carbon from mangrove charcoal. The activating agent used was phosphoric acid with 0.4-1.0 M and 2 hour immersion. The activation temperature was 650-725°C. The activated carbon of the research results was tested for its proximate level, iodine number, and specific surface area. The results showed that water content ranged from 0.11-0.38%, iodine number was around 769.075-1,019.087 mg/g. The highest iodine number was 1,019.87 mg/g and the surface area of 354.977 m2/g that occurred at 725 °C with activator concentration of 1 M. Proximate analysis, iodine number, and specific surface area of activated carbon from mangrove charcoal have met Indonesian National Standardization (SNI 06-3730-1995).

Ecosystem carbon stock of a tropical mangrove forest in North Sulawesi, Indonesia

Recent studies have highlighted the valuable role played by mangrove forests in carbon sequestration and storage. Although Indonesia accounts for a large proportion of global mangrove area, knowledge on the carbon stock and sources in the Indonesian mangrove is still limited. In this study, we quantified the ecosystem organic carbon (OC) stock and its spatial variation at an oceanic mangrove in Wori, North Sulawesi, Indonesia. The sources of soil OC were also investigated. The results showed that the mangrove soil had a substantial OC stock containing 15.4 kg/m2 (calculated by carbon) in the top 50 cm soil, and represented the majority of the ecosystem OC stock at the Wori mangrove. The mangrove biomass and ecosystem OC stock were 8.3 kg/m2 and 23.7 kg/m2, respectively. There was no significantly difference in the soil OC stock among the stations with difference distances offshore, while the highest mangrove biomass OC stock was found at the seaward station. Isotope mixing calculations showed that the rich OC in mangrove soils was attributed to the accumulated autochthonous mangrove source while the suspended organic matter in tidal water and the mangrove-adjacent seagrass contributed less than 20% to the soil OC. The results further demonstrated the importances of the oceanic mangrove in carbon storage and the mangrove plants in contributing OC to their soils.

KOMPOSISI JENIS POHON DAN STRUKTUR TEGAKAN HUTAN MANGROVE DI DESA PANTAI BAHAGIA KECAMATAN MUARA GEMBONG KABUPATEN BEKASI PROVINSI JAWA BARAT

Mangrove forest ecosystems have a very important role for humans and the environment, so to prevent further damage needs to be done identification of tree species composition and structure of mangrove forest stands. For the purpose of this research is to know the composition of tree species from the level of seedling to the tree and to know the structure of its stand. Data collection methods used are Inventory of mangrove species using Indonesian mangrove determination keychain and data analysis by calculating important value index, Diversity index, relative frequency and horizontal structure analysis. The results showed that the largest tree density structure was Rhizophora mucronata of 225 trees / hectare, Avicennia marinna of 145 trees / hectare, Sonneratia alba of 65 trees / hectare and Avicennia officinalis of 65 trees / hectare and had only one crown layer namely strata A which is dominated by Rhizophora mucronata tree which has an average free height of 9 m branch with total height 12 m and diameter 29 cm. For this reason, planting efforts need to be increased to increase the number and composition for coastal protection.