Indicator Of Biological Aging Research Articles

Inflammaging, a targetable pathway for preventing cardiovascular diseases.

Inflammaging, characterized by persistent chronic inflammation in older adults, has emerged as a critical factor linked to age-related diseases such as cardiovascular diseases (CVDs), metabolic disorders, and cognitive decline, which collectively contribute to the leading causes of death globally. Elevated levels of cytokines, chemokines, and others inflammatory mediators characterize inflammaging and serve as indicators of biological age. Among the causes of inflammaging, deterioration of the immune system, mitochondrial dysfunction, dysbiosis, accumulation of DAMPs, together with genetic or epigenetic factors, contribute to inflammaging not only in CVD but also in other age-related conditions. This review examines the causes and consequences of inflammaging, particularly its implications for atherosclerosis and heart failure with preserved ejection fraction (HFpEF) and explores potential strategies to mitigate it in the onset of CVD.

Contextualizing aging clocks and properly describing biological age.

Usage of the phrase "biological age" has picked up considerably since the advent of aging clocks and it has become commonplace to describe an aging clock's output as biological age. In contrast to this labeling, biological age is also often depicted as a more abstract concept that helps explain how individuals are aging internally, externally, and functionally. Given that the bulk of molecular aging is tissue-specific and aging itself is a remarkably complex, multifarious process, it is unsurprising that most surveyed scientists agree that aging cannot be quantified via a single metric. We share this sentiment and argue that, just like it would not be reasonable to assume that an individual with an ideal grip strength, VO2 max, or any other aging biomarker is biologically young, we should be careful not to conflate an aging clock with whole-body biological aging. To address this, we recommend that researchers describe the output of an aging clock based on the type of input data used or the name of the clock itself. Epigenetic aging clocks produce epigenetic age, transcriptomic aging clocks produce transcriptomic age, and so forth. If a clock has a unique name, such as our recently developed epigenetic aging clock CheekAge, the name of the clock can double as the output. As a compromise solution, aging biomarkers can be described as indicators of biological age. We feel that these recommendations will help scientists and the public differentiate between aging biomarkers and the much more elusive concept of biological age.

Identifying critical age and gender-based metabolomic shifts in a Japanese population of the Tohoku Medical Megabank cohort

Understanding the physiological changes associated with aging and the associated disease risks is essential to establish biomarkers as indicators of biological aging. This study used the NMR-measured plasma metabolome to calculate age-specific metabolite indices. In doing so, the scope of the study was deliberately simplified to capture general trends and insights into age-related changes in metabolic patterns. In addition, changes in metabolite concentrations with age were examined in detail, with the period from 55–59 to 60–64 years being a period of significant metabolic change, particularly in men, and from 45–49 to 50–54 years in females. These results illustrate the different variations in metabolite concentrations by sex and provide new insights into the relationship between age and metabolic diseases.

Decreased cobalamin sensitivity and biological aging acceleration in the general population

BackgroundThe evidence on the association between cobalamin (Cbl) and aging or relevant outcomes is limited and controversial. We aimed to investigate the relationships between cobalamin intake- and function-related biomarkers and biological aging. MethodsThe study encompassed 22,812 participants aged 20 years and older from the National Health and Nutrition Examination Survey. A panel of biomarkers or algorithms was used to assess biological aging, including Klemera–Doubal Age Acceleration (KDMAccel), Phenotypic age acceleration (PhenoAgeAccel), telomere length, α-Klotho, and PhenoAge advancement. Weighted generalized linear regression analysis was used to assess the associations between cobalamin-intake biomarkers (serum cobalamin, cobalamin intake from food, cobalamin supplement use, serum methylmalonic acid [MMA], and homocysteine [Hcy]) and function-related biomarkers (functional cobalamin deficiency and cobalamin insensitivity index). ResultsAmong the 22,812 individuals, the weighted mean (SE) age was 48.3 (0.2) years and 48.0% were males. Unexpectedly, serum and dietary cobalamin as well as serum MMA and Hcy levels were positively associated with most indicators of biological aging. Cobalamin sensitivity was assessed by the combination of binary Cbllow/high and MMAlow/high or Hcylow/high (cutoff values: 400 pg/mL for cobalamin, 250 nmol/L for MMA, and 12.1 μmol/l for Hcy) and a newly constructed cobalamin insensitivity index (based on the multiplicative term of serum cobalamin and serum MMA or Hcy). The multivariable-adjusted β (95%CIs) of KDMAccel in the MMAlowCbllow, MMAlowCblhigh, MMAhighCbllow, and MMAhighCblhigh groups were reference, 0.27 (0.03 to 0.51), 0.85 (0.41 to 1.29), and 7.97 years (5.77 to 10.17) respectively, which were consistent for the combination of serum Hcy and cobalamin. Both cobalamin insensitivity indices were robustly associated with biological aging acceleration in a dose-response pattern (each p < 0.001). ConclusionsDecreased cobalamin sensitivity but not cobalamin insufficiency might be associated with biological aging acceleration. Further studies would improve understanding of the underlying mechanisms between decreased cobalamin sensitivity and biological aging acceleration.

A Novel Method for Acquired Sex Chromosome Mosaicism.

The phenomenon of sex chromosome loss from hematopoietic cells is an emerging indicator of biological aging. While many methods to detect this loss have been developed, enhancing the field, these existing methods often suffer from being labor-intensive, expensive, and not sufficiently sensitive. To bridge this gap, a novel and more efficient technique is developed, named the SinChro assay. This method employs multiplexed single-cell droplet PCR, designed to detect cells with sex chromosome loss at single-cell resolution. Through the SinChro assay, the age-dependent increase in Y chromosome loss in male blood is successfully mapped. The age-dependent loss of the X chromosome in female blood is also identified, a finding that has been challenging with existing methods. The advent of the SinChro assay marks a significant breakthrough in the study of age-related sex mosaicism. Its utility extends beyond blood analysis, applicable to a variety of tissues, and it holds the potential to deepen the understanding of biological aging and related diseases.

Relationship of dietary natural folate and synthetic folic acid co-exposure patterns with biological aging: findings from NHANES 2003-2018.

Background: The mandatory folic acid fortification program in the United States has inevitably exposed most Americans to both natural folate and synthetic folic acid. We aim to examine the association of dietary folate co-exposure patterns with biological aging indicators. Methods: A total of 18 889 participants were enrolled from 2003 to 2018. Dietary intake of folate from diverse sources was evaluated by 24-hour dietary recall. Biological aging indicators were developed based on age-related clinical indicators, including the phenotypic age (PA), Klemera-Doubal method (KDM), homeostatic dysregulation (HD), and allostatic load (AL). The unsupervised K-means clustering method, logistic regression model, and restricted cubic spline (RCS) regression model were used to explore the relationship of natural folate and synthetic folic acid co-exposure with biological aging indicators. Results: The results indicated that higher intake of total folate, dietary folate, and food natural folate was associated with lower PA [OR = 0.75 (0.64, 0.88); OR = 0.79 (0.70, 0.90); OR = 0.65 (0.57, 0.75)], KDM [OR = 0.63 (0.53, 0.75); OR = 0.80 (0.65, 0.98); OR = 0.62 (0.49, 0.77)], HD [OR = 0.69 (0.56, 0.84); OR = 0.78 (0.67, 0.92); OR = 0.78 (0.68, 0.90)], and AL [OR = 0.69 (0.58, 0.82); OR = 0.73 (0.63, 0.85); OR = 0.74 (0.62, 0.90)], consistently. Four co-exposure patterns were generated based on the intake of folate from diverse sources, as follows: "low folate exposure group" to cluster 1, "dietary folate exposure group" to cluster 2, "mixed source high folate exposure group" to cluster 3, and "mixed source excessive folate exposure group" to cluster 4. Compared with cluster 1, participants in cluster 2 are associated with lower biological age indicators (ORPA = 0.82 [0.72, 0.93]; ORKDM = 0.58 [0.47, 0.70]; ORHD = 0.85 [0.75, 0.97]; ORAL = 0.87 [0.77, 0.98]), while participants in cluster 3 and cluster 4 are not. Conclusion: For individuals subjected to folic acid fortification programs, a higher intake of dietary folate, especially natural folate, coupled with a lower consumption of folic acid supplements, was found to be associated with lower biological age indicators.

Health-promoting effect for students from physical loads of speed and strength orientation.

Aim: To investigate the impact of speed and strength physical loads on promoting health and reducing the biological age of student youth. Materials and Methods: The research involved 180 students aged 18-19. The first stage provided for the study of the indicators of the biological age of 120 students, and the second stage covered substantiation of the methodology for martial arts training sessions, based on physical loads of speed and strength orientation. To test its effectiveness, 60 students were involved (30 were in the experimental group (EG), 30 - in the control group (CG)). Results: The methodology for martial arts training sessions was developed and tested. In the dynamics of the experiment, there is a tendency to a decrease in the difference between the biological and the passport age, but these changes were unreliable in the CG, while a statistically significant decrease in this difference was recorded in the EG. In addition, there were significant changes in the EG in the following indicators: inspiratory breath holding (11.6 %), subjective health assessment (38.5 %), and static balancing (20.0 %). Conclusions: The use of physical loads of speed and strength orientation in the course of martial arts training has established a health-promoting effect and positive dynamics of the biological age indicators of student youth. The results of the research can be implemented in physical education and the process of sports improvement of students in higher educational institutions.

The study of the biological age of university teachers in the period of urgent transition to distance education as a result of pandemic

In the article, the biological age of university teachers was studied (using the express method of V.P. Voytenko), a total of 127 people (64 women, 63 men) were examined, they were divided into three groups according to their age (1st group ‒23-39 years old, 2nd group ‒40-59 years old, 3rd group ‒60 years old and older).A special health self-assessment questionnaire and a number of physiological indicators and mathematical formulas were used to calculate the value of biological age (BA);the appropriate biological age value (ABA) was calculated; he obtained biological age and the corresponding biological age were compared, it was determined how many years the subjects' aging rate was ahead or behind their peers. This approach makes it possible to divide people of the same age into several degrees according to the degree of “age wear”, as well as according to the “reserve” of health (degrees I and II ‒slow aging, III ‒BA corresponds to the population standard, IV and V ‒accelerated aging). The research was conducted during the transition of universities to distance education during the pandemic (October-November, 2020). More than half of the teachers (55.90%) were found to be experiencing accelerated aging (grade IV-V of BA), and this was more common among men than among women. Among those examined, the rate of aging is evident in the youngest group of teachers. Due to the pandemic, in the period of urgent transition from the usual traditional educational format to distance learning, the accelerated rate of aging according to biological age indicators was observed in almost all groups of teachers: 23%-80%. And there is a conclusion that the gender and age of the subjects affect the biological age.

Unraveling the causal genes and transcriptomic determinants of human telomere length

Telomere length (TL) shortening is a pivotal indicator of biological aging and is associated with many human diseases. The genetic determinates of human TL have been widely investigated, however, most existing studies were conducted based on adult tissues which are heavily influenced by lifetime exposure. Based on the analyses of terminal restriction fragment (TRF) length of telomere, individual genotypes, and gene expressions on 166 healthy placental tissues, we systematically interrogate TL-modulated genes and their potential functions. We discover that the TL in the placenta is comparatively longer than in other adult tissues, but exhibiting an intra-tissue homogeneity. Trans-ancestral TL genome-wide association studies (GWASs) on 644,553 individuals identify 20 newly discovered genetic associations and provide increased polygenic determination of human TL. Next, we integrate the powerful TL GWAS with placental expression quantitative trait locus (eQTL) mapping to prioritize 23 likely causal genes, among which 4 are functionally validated, including MMUT, RRM1, KIAA1429, and YWHAZ. Finally, modeling transcriptomic signatures and TRF-based TL improve the prediction performance of human TL. This study deepens our understanding of causal genes and transcriptomic determinants of human TL, promoting the mechanistic research on fine-grained TL regulation.

Relationship between an ageing measure and chronic obstructive pulmonary disease, lung function: a cross-sectional study of NHANES, 2007–2010

ObjectivesChronic obstructive pulmonary disease (COPD) is a disease associated with ageing. However, actual age does not accurately reflect the degree of biological ageing. Phenotypic age (PhenoAge) is a new indicator...

Reconsideration of the current models of estimated kidney function-based drug dose adjustment in older adults: The role of biological age.

Human lifespan has increased from a median of 46.5 years in 1950 to 71.7 years in 2022. As people age, one of the inevitable consequences is a decline in kidney function and glomerular filtration rate (GFR) which can have direct or indirect effects on the pharmacokinetic and pharmacodynamic profiles of many drugs. Numerous equations have been developed to generate estimated GFR (eGFR) using the two principal biomarkers: serum creatinine and serum cystatin C. However, the trajectory of changes with aging is dissimilar in these equations. In addition, there is recognition that chronological age (lifespan) often does not reflect biological age (healthspan) as an essential parameter in kidney function equations. In the past decade, there has been an increasing interest in quantifying biological age and new commercially available assays have entered the marketplace. In this narrative review, we illustrate how dominant equations of eGFR model the fractional change in this parameter very differently across chronological age. In addition, we review various biological age indicators (aging clocks) and challenges to their application in clinical practice. Importantly, by comparing vancomycin's mean clearance as a drug with limited metabolism and unchanged elimination between two age milestones in some recent population pharmacokinetic models, we show how efforts to quantify kidney function in older adults optimally remain under-explored, particularly those at the upper end of their lifespan. We also propose considering new models that integrate biological age as a new pathway to improve precision drug dosing in older adults.

Associations of four biological age markers with child development: A multi-omic analysis in the European HELIX cohort.

While biological age in adults is often understood as representing general health and resilience, the conceptual interpretation of accelerated biological age in children and its relationship to development remains unclear. We aimed to clarify the relationship of accelerated biological age, assessed through two established biological age indicators, telomere length and DNA methylation age, and two novel candidate biological age indicators, to child developmental outcomes, including growth and adiposity, cognition, behavior, lung function and the onset of puberty, among European school-age children participating in the HELIX exposome cohort. The study population included up to 1173 children, aged between 5 and 12 years, from study centres in the UK, France, Spain, Norway, Lithuania, and Greece. Telomere length was measured through qPCR, blood DNA methylation, and gene expression was measured using microarray, and proteins and metabolites were measured by a range of targeted assays. DNA methylation age was assessed using Horvath's skin and blood clock, while novel blood transcriptome and 'immunometabolic' (based on plasma proteins and urinary and serum metabolites) clocks were derived and tested in a subset of children assessed six months after the main follow-up visit. Associations between biological age indicators with child developmental measures as well as health risk factors were estimated using linear regression, adjusted for chronological age, sex, ethnicity, and study centre. The clock derived markers were expressed as Δ age (i.e. predicted minus chronological age). Transcriptome and immunometabolic clocks predicted chronological age well in the test set (r=0.93 and r=0.84 respectively). Generally, weak correlations were observed, after adjustment for chronological age, between the biological age indicators.Among associations with health risk factors, higher birthweight was associated with greater immunometabolic Δ age, smoke exposure with greater DNA methylation Δ age, and high family affluence with longer telomere length.Among associations with child developmental measures, all biological age markers were associated with greater BMI and fat mass, and all markers except telomere length were associated with greater height, at least at nominal significance (p<0.05). Immunometabolic Δ age was associated with better working memory (p=4 e-3) and reduced inattentiveness (p=4 e-4), while DNA methylation Δ age was associated with greater inattentiveness (p=0.03) and poorer externalizing behaviors (p=0.01). Shorter telomere length was also associated with poorer externalizing behaviors (p=0.03). In children, as in adults, biological aging appears to be a multi-faceted process and adiposity is an important correlate of accelerated biological aging. Patterns of associations suggested that accelerated immunometabolic age may be beneficial for some aspects of child development while accelerated DNA methylation age and telomere attrition may reflect early detrimental aspects of biological aging, apparent even in children. UK Research and Innovation (MR/S03532X/1); European Commission (grant agreement numbers: 308333; 874583).

Exploratory and confirmatory factor analyses identify three structural dimensions for measuring physical function in community-dwelling older adults.

Physical function is a strong indicator of biological age and quality of life among older adults. However, the results from studies exploring the structural dimensions of physical function are inconsistent, and the measures assessed vary greatly, leading to a lack of comparability among them. This study aimed to construct a model to identify structural dimensions that are suitable and best assess physical function among community-dwelling adults 60-74 years of age in China. This study was conducted in 11 communities in Shanghai, China, from May to July 2021. A total of 381 adults 60-74 years of age were included in the study. Measured physical function data were used in factor analyses. Data collected from individuals were randomly assigned to either exploratory factor analysis (EFA) (n=190) or confirmatory factor analysis (CFA) (n=191). The statistical software used in the study was SPSS for EFA and AMOS for CFA. To test the properties of the structural dimension model of physical function, various fit indices, convergent validity, and discriminant validity were calculated. The EFA results derived seven indicators in three factors, with 58.548% of the total variance explained. The three factors were mobility function (three indicators), which explained 26.380% of the variance, handgrip strength and pulmonary function (two indicators), which explained 19.117% of the variance, and muscle strength (two indicators) which explained 13.050% of the variance. The CFA indicated that this model had an acceptable fit (χ2/df ratio, 2.102; GFI, 0.967; IFI, 0.960; CFI, 0.959; and RMSEA, 0.076), and the criteria for convergent validity and discriminability were also met by the model. The constructed structural dimension model of physical function appeared to be a suitable and reliable tool to measure physical function in community-dwelling adults aged 60-74 years in China. The structural dimension indicators identified by this model may help sports medicine experts and healthcare providers offer more targeted interventions for older adults to reverse or slow the decline of physical function and to offer actionable targets for healthy aging in this population.

Telomere length- A biological marker of ageing

Telomere length is an indicator of biological age, influenced mainly by oxidative stress and inflammatory conditions. Telomere length shortens with advancing age, however in certain situations this shortening expedites with early senile changes while in other situations it can be delayed resulting in slowing of ageing. Ageing is a factor associated with a number of diseases and also treatment decision of lethal diseases is directly influenced by the age. However, understanding the difference of chorological versus biological age is essential. It is also essential to determine cut-offs of the age by using telomere length.

DNA methylation states in supercentenarians

Molecular mechanisms contributing to healthy ageing and longevity are not yet completely understood.1 One potential mechanism involved is DNA methylation,1 a process that serves as a dynamic regulator of cell function and can also be a means to define epigenetic age, an indicator of biological age.1,2 Studying the epigenetic patterns of supercentenarians has special relevance in longevity research because epigenetics is the connection between the environment and the inherited genome.1

Intra-Positional and Inter-Positional Differences in Agility Tests among Youth Female Volleyball Players

In their structure, indoor sports require quick changes of movement direction. Players should possess a high level of ability to change the direction and speed of movement in response to a stimulus, which is one of the major prerequisites for a high level of performance. During a volleyball match, players are involved in different defensive and offensive activities in which agility plays an important role. The aim of this study was to determine the differences between less successful and more successful youth female volleyball players, as well as their inter-positional and intra-positional differences in agility. The study was conducted on a sample of 204 youth female volleyball players whose mean chronological age was 14.11±0.84 years. The players were divided into 5 subgroups according to their playing position (setters, opposite players, passer-hitters, middle blockers, and libero players). The variable sample used to assess agility consisted of 3 tests: Side steps, 9-3-6-3-9 test, and Step-hop test. Methods of data analysis included the determination of metric characteristics for all three agility tests. The analysis of covariance revealed a significant impact of biological age indicators on all agility tests. Furthermore, discriminant analysis of differences showed that more successful volleyball players achieved better results in all three agility tests. High reliability of the test was determined by Cronbach’s Alpha coefficient (0.87-0.96). The results of this study showed a positive effect of agility tests in differentiating less successful and more successful players regardless of their position. More successful players achieved better results than less successful players in all tests. Greater biological maturity and training experience represent great competitive advantage in this age group. The obtained inter-positional and intra-positional differences and the influence of the biological age indicator on inter-positional and intra-positional differences in agility tests imply that higher biological maturity and training experience are great competitive advantages in this age group. The primary goal of training in younger age groups should not be the current success in the competition, but rather the preparation for achieving the best possible results in senior competition.

Միկրոալիքային ճառագայթման հետ շփում ունեցող զինծառայողների կենսաբանական տարիքի և ծերացման տեմպի հարցի շուրջ / On the issue of biological age and the aging rate of the military personnel exposed to microwave radiation

The 21st century is characterized by the rapid development of radio­electronics, which has brought on and continues to bring on a natural increase in the number of people exposed to ultrahigh-frequency electromagnetic radiation during the production, testing, operation and repair of generators of such radiation. They have become extensively exploited in military affairs, meteorology, navigation, space and other scientific research. The problem becomes even more urgent due to the widespread use of equipment operating with the use of non-ionizing radiation in everyday life. For that reason, the problem of the impact of such radiation on the human body becomes urgent, depending on the time of exposure (the length of professional exposure to radiation). Currently, it has been established that the biological age and the aging rate are integral criteria for assessing the state of the human body working under the influence of a number of physical and chemical factors caused by microwave radiation. The study is targeted at developing scientifically substantiated preventive measures aimed at preserving the health and working capacity of this group of people. In this regard, the authors studied the aging rates of individuals (100 persons aged 18-27), professionally exposed to microwaves of non-thermal intensity. In the course of the study, changes in the value of biological age and the aging rate were analyzed depending on the calendar age and professional experience. The data obtained revealed the following patterns: slowing down of aging, which, apparently, could be considered as body response to microwave exposure; variations in the indicators of biological age and the aging rate, taking into account professional experience, which may indicate a certain process of adaptive restructuring of the body going on against the background of age-related changes and prolonged professional activity. In these individuals the so-called type of “health accumulation” is recorded. Based on the results of the study, specific recommendations have been developed to improve the health of people who are professionally exposed to non-thermal non-ionizing ultra-high-frequency electromagnetic radiation. «HAIKAKAN BANAK» DEFENSE-ACADEMIC JOURNAL 2023 © All Rights Reserved

Increased serum lipofuscin associated with leukocyte telomere shortening in veterans: a possible role for sulfur mustard exposure in delayed-onset accelerated cellular senescence.

Sulfur mustard (SM) is a toxic gas that causes chronic inflammation and oxidative stress leading to cell senescence. This study aimed to evaluate two indicators of biological aging (i.e., serum lipofuscin level and leukocyte telomere length) and assess their relationship based on the severity of SM exposure in the long term. The study was performed on two groups of male participants. 1) SM-exposed group (exposed to SM once in 1987), 73 volunteers. 2) Non-exposed group, 16 healthy volunteers. The SM-exposed group was categorized into three subgroups based on the severity of SM exposure and body damage (asymptom, mild, and severe). The blood sample was prepared from members of each group. The serum lipofuscin, TGF-β, malondialdehyde (MDA), c-reactive protein (CRP), and leukocyte telomere length (TL) were measured in all participants. The MDA level was increased in the SM-exposed group (mean = 39.6 µM, SD = 16.5) compared to the non-exposed group (mean = 21.1 µM, SD = 10.3) (P < 0.05). The CRP level was also increased in the SM-exposed group (mean = 5.12 mg/l, SD = 3.36) compared to the non-exposed group (mean = 3.51 mg/l, SD = 1.21), while the TGF-β level was decreased (P < 0.05) in the SM-exposed group (mean = 52.6 pg/ml, SD = 18.7) compared to the non-exposed group (mean = 68.9 pg/ml, SD = 13.8). The relative TL was shorter in the SM-exposed group (mean = 0.40, SD = 0.28) than in the non-exposed group (mean = 2.25, SD = 1.41) (P < 0.05). The lipofuscin level was higher in the total SM-exposed group (mean = 1.44 ng/ml, SD = 0.685) than in the non-exposed group (mean = 0.88 ng/ml, SD = 0.449) (P < 0.05). The MDA and CRP levels were increased in the SM-exposed subgroups of asymptom, mild, and severe than the non-exposed group, while TGF-β level and TL were decreased in those subgroups. The lipofuscin level was higher in the SM-exposed subgroups of mild and severe than in the non-exposed group. The regression analysis determined a negative correlation between lipofuscin level and TL. The lipofuscin/TL ratio was higher in the total SM-exposed group (mean = 6.36, SD = 5.342) than in the non-exposed group (mean = 0.51, SD=0.389). This ratio was also higher in the SM-exposed subgroups of asymptom, mild, and severe than in the non-exposed group. The lipofuscin/TL ratio did not differ between mild and severe subgroups. The delayed toxicity of SM is associated with chronic oxidative stress, continuous inflammatory stimulation, increased lipofuscin, and telomere shortening. Future studies are needed to verify the suitability of serum lipofuscin to telomere length ratio in determining the severity of SM toxicity.

Indicators of functional integrity in production animals

An ability to describe the quality of lived experience across the course of an animal’s life is of increasing interest in studies on wellbeing in farm animals. Integrity of physical functions is an important aspect of the tripartite model of wellbeing: feeling, functioning and interacting well. This review examines measures of functional integrity as potential indicators of functional (eudaimonic) wellbeing. Copious literature shows that coping with day-to-day fluctuations in the environment influences (1) periodicity of biorhythms, (2) allocation of resources to growth, defence and repair, and (3) rate of biological aging. Here we review opportunities for assessing functional integrity through analysis of (1) the dynamic status of physiological and behavioural variables, (2) the accretion of resources in production tissues (milk, eggs, body mass, offspring) and physical structures (fibre, hoof, bone, tooth, placoid scale, otolith, beak), and (3) biological age. A reduction in periodicity of biorhythms, complexity of behaviours, or uniformity of trajectories of growth and production is often associated with poorer current and future health outcomes and shorter functional longevity. These analytical approaches are grounded in statistical measures of stability in complex dynamic systems. Additionally, the cumulative effect of wear and tear on biological age can be assessed via telomere length, DNA methylation, biomarkers of allostatic load, hair cortisol, and physiological functional capacity. Timeseries of behaviour, physiology and accretion provide a richer record of functional integrity than do cumulative indicators of biological age. Indicators of integrity are needed for each phase of the animal’s life course including attainment of developmental competencies, stability of mature performance, and rate of decline with advancing age, to provide a comprehensive record across the whole of the animal’s life. The utility of measures may differ between real-time and retrospective assessments and between application to every individual versus sentinels. Further validation is required for interpretating measures of functional integrity as indicators of the functional aspect of wellbeing, together with determining their practicality and reliability before translation to commercial settings is feasible. A single type of indicator of functional wellbeing (e.g. DNA methylation) is unlikely to have utility for all life stages, species, production systems, and environments.

Influence of Aqua Fitness Training Sessions on Improving the Biological Age Indicators in Overweight Female Students

Aim: To experimentally test the influence of aqua fitness training sessions on reducing the rate of aging in overweight female students. Materials and Methods: The research involved 25 female students aged 17-21 who were overweight. All female students were healthy, not obese, and overweight was associated with their unhealthy diet and insufficient motor activity. These female students were formed into experimental (EG, n = 12) and control (CG, n = 13) groups. Aqua fitness was used in the physical education of the EG students, while the CG students were engaged in dance aerobics. Biological age was studied using V. P. Voitenko’s method, which involves assessing the indicators of body weight, arterial blood pressure, static balancing time, and the index of health selfassessment. Results: The author’s methodology of aqua fitness training sessions was developed and experimentally tested, the content of which included specific aerobic, strength and coordination exercises performed against the background of constant tension of the abdominal muscles and reduced load on the joints in the aquatic environment. The positive influence of aqua fitness training sessions according to the author’s methodology on the indicators of body weight, static balancing time, and health self-assessment index was revealed. Conclusions: As a result of aqua fitness training, the EG students had a more significant decrease in the rate of aging of the body than the CG students, which confirms the enhanced health effect of aqua fitness training sessions.