Indicator Of Aggressive Behavior Research Articles

Comprehensive Genome and Transcriptome Analysis Identifies SLCO3A1 Associated with Aggressive Behavior in Pigs.

Copy number variation (CNV) represents a significant reservoir of genetic diversity within the genome and exhibits a strong association with economically valuable traits in livestock. The manifestation of aggressive behavior in pigs has detrimental effects on production efficiency, immune competency, and meat quality. Nevertheless, the impact of CNV on the aggressive behavior of pigs remains elusive. In this investigation, we employed an integrated analysis of genome and transcriptome data to investigate the interplay between CNV, gene expression changes, and indicators of aggressive behavior in weaned pigs. Specifically, a subset of pigs comprising the most aggressive pigs (MAP, n = 12) and the least aggressive pigs (LAP, n = 11) was purposefully selected from a herd of 500 weaned pigs following a mixing procedure based on their composite aggressive score (CAS). Subsequently, we thoroughly analyzed copy number variation regions (CNVRs) across the entire genome using next-generation sequencing techniques, ultimately revealing the presence of 6869 CNVRs. Using genome-wide association study (GWAS) analysis and evaluating variance-stabilizing transformation (VST) values, we successfully identified distinct CNVRs that distinguished the MAP and LAP counterparts. Among the prioritized CNVRs, CNVR-4962 (designated as the top-ranked p-value and VST value, No. 1) was located within the Solute Carrier Organic Anion Transporter Family Member 3A1 (SLCO3A1) gene. The results of our analyses indicated a significantly higher (p < 0.05) copy number of SLCO3A1 in the MAP compared to the LAP. Furthermore, this increased copy number exhibited a positive correlation with the CAS of the pigs (p < 0.05). Furthermore, we integrated genomic data with transcriptomic data from the temporal lobe to facilitate the examination of expression quantitative trait loci (eQTL). Importantly, these observations were consistent with the mRNA expression pattern of SLCO3A1 in the temporal lobe of both MAP and LAP (p < 0.05). Consequently, our findings strongly suggest that CNVs affecting SLCO3A1 may influence gene expression through a dosage effect. These results highlight the potential of SLCO3A1 as a candidate gene associated with aggressive traits in pig breeding programs.

No mercy for victims: Exploring the link between dark personality traits, aggressive video game behavior, and severe traffic violations.

This study investigates the relationship between dark personality traits, aggressive behavior in violent video games, and severe traffic violations among 200 driving offenders from Tehran, Iran, participating in a rehabilitation program. Participants engaged in a computerized shooting decision task, where their tendency to shoot unarmed targets (innocent victims), compared to armed targets (criminals), was used as an indicator of aggressive behavior toward innocent victims. Additionally, they completed self-report measures of narcissism, Machiavellianism, psychopathy, and sadism to evaluate the impact of Dark Tetrad personality traits on their behavior. Bivariate analyses revealed associations between Dark Tetrad personality traits and aggressive behavior in the video game with serious traffic offenses. Multivariate analyses identified Machiavellianism, sadism, and aggressive behavior in video games as significant predictors of severe traffic offenses. The results suggest that dark personality traits and aggressive behavior in video games may aid in better identifying road traffic offenders with the most severe violations. Potential implications for preventing repeated traffic offenses by tailoring rehabilitation programs are discussed.

The Variation of Serotonin Values in Dogs in Different Environmental Conditions.

Simple SummaryThe multiple implications of serotonin in behavior manifestations have shaped the goal of the present study, which was to evaluate the variation of serum serotonin levels in different experimental groups of dogs to establish whether serum serotonin levels could serve as indicators of aggressive behavior, especially when adoption is considered. The experimental groups were divided into three variants: Variant 1—two groups of medium (n = 6) and small (n = 4) breed shelter dogs; Variant 2—dogs with owners (n = 15) and dogs without owners but in foster care (n = 10), after administration of pre-spaying/neutering anesthesia; and Variant 3—dogs in different behavioral states (n = 8), classified as follows: M1—happy, M2—aggressive, M3—calmed status, post-exposure to a stressful situation, compared to the reference time referred to as M0. Significant results were found between M1 and M2 (p ≤ 0.05, decrease of serotonin by 89.61 ng/mL), as well as between M2 and M3 (p ≤ 0.008, increase by 112.78 ng/mL). Following anesthesia, the average mean serotonin values were significantly lower (p ≤ 0.003), by 63.85 ng/mL, in stray dogs compared to dogs with owners, leading to a presumptive conclusion that serotonin levels could serve as indicators for potentially aggressive behaviors.Serotonin is considered to be the neurotransmitter that controls several types of behavior: aggressiveness, impulsivity, food selection, stimulation, sexual behavior, reaction to pain, and emotional manifestations. The aim of this study was to determine the serotonin values in 43 dogs, divided into three different experimental variants: (1) between two groups of medium (n = 6) and small (n = 4) breed shelter dogs; (2) in dogs with (n = 15) and without (n = 10) owners after administration of pre-spaying/neutering anesthesia; (3) in different behavioral states (n = 8) classified as follows: M1—happy, M2—aggressive, M3—calmed status, post-exposure to a stressful situation, compared to the reference time referred to as M0. There were no significant differences (p ≥ 0.05) regarding the serotonin values between the two groups of medium and small breed shelter dogs. Following anesthesia, the average mean serotonin values were significantly lower (p ≤ 0.003), by 63.85 ng/mL, in stray dogs compared to dogs with owners. No significant differences (p ≥ 0.05) were found when comparing the reference time M0 to M1, M2, and M3. The differences decreased significantly (p ≤ 0.05), by 89.61 ng/mL, between M1 and M2 and increased significantly (p ≤ 0.008), by 112.78 ng/mL, between M2 and M3.

Epithelial membrane antigen and DOG1 expression in minor salivary gland tumours

Epithelial membrane antigen (EMA) and DOG1 are used as marker of epithelial cells, particularly the luminal cells, of salivary gland tumours. The aim of this study was to compare the EMA and DOG1 expression in tumours of minor salivary glands. Cases of pleomorphic adenoma (PA), basal cell adenoma (BCA), canalicular adenoma (CA), adenoid cystic carcinoma (ACC), polymorphous adenocarcinoma (PAC), mucoepidermoid carcinoma (MEC) and epithelial-myoepithelial carcinoma (EMC) were submitted to immunohistochemistry for EMA and DOG1. In PA and BCA, EMA and DOG1 were observed in luminal cells, while in CA the tumour cells were negative for both proteins. The EMA and DOG1 pattern expression detected in EMC was similar to that one observed in benign tumours. In ACC, both myoepithelial e epithelial expressed EMA and DOG-1. PAC tumour cells were only positive for DOG1, whereas MEC were only positive for EMA. In conclusion, EMA and DOG1 expression in benign salivary gland tumours was similar to normal salivary gland tissue and can be used as good marker of tumoral cells derived from intercalated ducts or its progenitor cells, while in malignant salivary gland tumours EMA expression is, however, better used as an indicator of aggressive behavior than a marker of luminal cells.

Does Facial Width-to-Height Ratio Predict Aggressive Behavior in Association Football?

A growing body of research has emphasized the role of facial structures in affecting human social behavior. In particular, the facial width-to-height ratio (fWHR) was found to predict aggressive behavior, most notably in men of low social status. The current study aimed at broadening the insights into the role of fWHR in sports regarding the indicators of aggressive behavior and performance. We questioned the impact of fWHR in association football by analyzing fouls committed by players, their (yellow and red) cards received, and their goals scored and assists in the German ( n = 278) and Austrian ( n = 194) first division in the 2016–2017 season (306/180 matches). The market values of players were used as a measure of social status. Generalized estimating equation models taking negative binomial distribution into account did not reveal any significant impact of fWHR on fouls committed, yellow and red cards received, or assists and goals scored. Even the consideration of players’ field positions, players’ body mass index (BMI), their market value, and the interactional term of players’ fWHR and players’ market value did not affect the results. The analyses refuted any impact of fWHR in association football both on parameters of aggressive behavior (fouls and cards received) and performance (goals and assists). Merely, players’ market value was found to predict players’ goals and assists. The current study adds to the empirical evidence encouraging a critical assessment and discussion of fWHR research in sports.

Loss of pRB in Conjunctival Squamous Cell Carcinoma: A Predictor of Poor Prognosis.

Conjunctival squamous cell carcinoma (SCC) is the most common tumor of conjunctival epithelium. It is associated with risk of permanent visual impairment and has the capability to recur, metastasize, and cause death. Deregulation of cell cycle control has been reported in a number of malignancies. The aim of the present study was to assess expression of G1/S cell cycle regulatory proteins [retinoblastoma protein (pRb)/P16/cyclin D1] in conjunctival SCC. Forty-four prospective cases of conjunctival SCC from a tertiary eye care referral center in northern India were included in this study. American Joint Committee on Cancer (AJCC) staging was performed and patients were followed up for 46±3.2 months. pRb loss was seen in 87% and overexpression of p16 and cyclin D1 in 36% and 66%, respectively. Kaplan-Meier analysis revealed reduced disease-free survival in patients with pRb loss (P=0.006). On univariate analysis, pRb loss (P=0.02), orbital invasion (P=0.03), and AJCC stage ≥T3 (P=0.03) emerged as significant high-risk features. On multivariate analysis pRb loss emerged as the most significant poor prognostic indicator in conjunctival SCC cases. Our findings suggest pRb loss to be a useful indicator of aggressive behavior and is recommended for identifying high-risk conjunctival SCC patients.

Hubungan Antara Kecenderungan Pola Asuh Otoriter (Authoritarian Parenting Style) dengan Gejala Perilaku Agresif Pada Remaja

Aggressive behavior is an action which aims to hurt or to injure someone else. Aggressive behavior could appear at any age, without exception for the adolescent. The adolescent is described as a storm and stress period, that is the emotional turbulence that accompanied by the decrease of ability to manage their emotion with the result that potentially to form an aggressive behavior. One of the factor that cause aggressive behavior is the authoritarian parenting style. The authoritarian parenting style done by the parents can bring some impacts for the children. The children which always prosecuted to do their parents’ wish will feel frustrated which makes them to act freely and behave aggressively when they are outside home ( Sarwono,1988).This research is purposed to know the significant relationship between tendency of authoritarian parenting style with indication of aggressive behavior in adolescents. The subject of this research is 258 adolescents at the age of 13 and 18 years old. The method used in this research is quantitative methods with correlational approach. The sampling technique used is multistage random sampling . The measuring tool that used is authoritative upbringing scale (31 aitem; rxx` = 0,915) and aggressive behavior scale (39 aitem; rxx` = 0,902).The main data in this research is analyzed by using spearman nonparametric correlation analysis with correlation value 0,314 and probability value 0,000. The supporting data is analyzed by using Independent Sample Test Analysis to see the difference of aggressive behaviour if it is observed from the age and the gender. The result of this research which analyzed by using Independent Sample Test indicate that there is no difference of aggressive behaviour based on the gender and age of research subject.Keywords : Authoritarian Parenting Style., Aggressive Behaviour, Adolescent.

Abstract 5155: Status and clinical relavence of G1/S cell cycle regulatory proteins in Ocular Surface Squamous Neoplasia

Abstract Purpose: Ocular Surface Squamous Neoplasia (OSSN) is a common tumour of ocular surface arising from the limbus, cornea or conjunctiva. It includes conjunctival intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC). Although confined to ocular surface, they have capability to recur (5-53%), metastasize (0-16%) and even cause death (8%).One of the most important issues still unresolved in the treatment and management of OSSN is the inability to determine factors causing disease progression. Identification of reliable biomarkers which could detect progression as well as identify high risk cases is an urgent necessity. The Cyclin D1/p16INK4a/pRb pathway plays a critical role in tumorigenesis and alterations in these proteins have been reported in various malignancies. This study was designed to explore the status of pRb, p16INK4a and cyclin D1 in various clinical stages of OSSN patients. Methods:Sixty-four histopathologically confirmed OSSN cases (44 squamous cell carcinomas and 20 conjunctival intraepithelial neoplasias) were included in this study. AJCC TNM staging was done and patients were followed up for 36 to 58 months (median 46 months). Immunohistochemical expression of total pRb (clone-13A10), phosphorylated Rb (clone-Phospho-Ser780), p16INK4a (clone-6H12), and cyclin D1 (clone-SP4) protein was evaluated. Kaplan-Meier survival and Cox regression analysis was done to assess the prognostic significance of these proteins. Results: Immunoexpression of the targeted proteins demonstrated loss /inactive form of pRb in 87% OSSN cases, p16INK4a and cyclin D1 overexpression in 28% in 63% cases respectively. From T1 to T3 AJCC category there was a progressive increase of p16INK4a (0%in T1 to 100% in T3) (P = 0.007) and cyclin D1 overexpression (25% in T1 to 88% in T4). Loss of pRb (75% in T1 to 100% in T4) (P = 0.03) was also seen. Loss of pRb expression was the earliest change which was detected in 75% cases in T1.The Kaplan Meier survival curves revealed loss of pRb (P = 0.0002, log rank analysis) as well as p16INK4a(P = 0.11, log rank analysis) and cyclin D1 over expression (P = 0.06, log rank analysis) adversely affected the disease free survival. pRb loss however emerged as the single most sensitive poor prognostic indicator in all AJCC stages of OSSN patients. Conclusion: G1/S cell cycle proteins (retinoblastoma, p16INK4aand cyclin D1) have prognostic relevance in OSSN patients. Of these pRb loss is the most useful indicator of aggressive behavior and is therefore recommended for detecting high risk OSSN patients. The dysregulation of p16INK4a, cyclin D1 and pRb proteins also plays an important role in pathogenesis of OSSN patients. Note: This abstract was not presented at the meeting. Citation Format: Sheetal Chauhan, Seema Sen, Anjana Sharma, Seema Kashyap, M. Vanathi, Neelam Pushker, Radhika Tandon. Status and clinical relavence of G1/S cell cycle regulatory proteins in Ocular Surface Squamous Neoplasia. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5155. doi:10.1158/1538-7445.AM2015-5155

Predicting aggressive behavior with the aggressiveness-IAT.

The Implicit Association Test (IAT, Greenwald, McGhee, & Schwartz, 1998) was adapted to assess the automatically activated (implicit) self-concept of aggressiveness. In three studies the validity of the Aggressiveness-IAT (Agg-IAT) was supported by substantial correlations with self-report measures of aggressiveness. After controlling for self-report measures of aggressiveness, the Agg-IAT accounted for 9-15% of the variance of three different indicators of aggressive behavior across three studies. To further explore the nomological network around the Agg-IAT we investigated its correlations with measures of social desirability (SD). Although not fully conclusive, the results across four studies provided some support for a weak negative correlation between impression management SD and aggressive behavior as well as the Agg-IAT. This result is in line with an interpersonally oriented self-control account of impression management SD. Individuals with high SD scores seem to behave less aggressively, and to show lower Agg-IAT scores. The one-week stability of the Agg-IAT was r = .58 in Study 4. Aggr. Behav. 41:65-83 2015. © 2014 Wiley Periodicals, Inc.

A classification tree approach for pituitary adenomas

It is difficult to evaluate the recurrence and progression potential of pituitary adenomas at presentation. The World Health Organization classification of endocrine tumors suggests that invasion of the surrounding structures, size at presentation, an elevated mitotic index, a Ki-67 labeling index higher than 3%, and extensive p53 expression are indicators of aggressive behavior. Nevertheless, Ki-67 and p53 labeling index evaluation is subject to interobserver variability, and their cutoff values are controversial. In the present study, the prognostic value of Ki-67 and p53 protein labeling indices and their correlation with clinical and radiologic parameters were evaluated using digital image analysis in a series of 166 pituitary adenomas in patients having undergone a follow-up of at least 6 years to evaluate the impact on the recurrence and progression potential of pituitary adenomas. The data were analyzed using the receiver operating characteristic curve and classification and regression tree analysis. The results showed that, in the unstratified data set, the commonly used threshold of the Ki-67 index of 3% has a high specificity (89.5%) but a low sensitivity (53.8%). Unsatisfactory performance results were obtained by performing receiver operating characteristic curve analysis on the p53 labeling index. On the contrary, the classification and regression tree analysis-derived tree demonstrated that each pituitary adenoma subtype has specific prognostic factors. Specifically, the Ki-67 labeling index is a useful prognostic factor in nonfunctioning, adrenocorticotropin, and prolactin adenomas, but with different thresholds. In conclusion, our study emphasizes that the term pituitary adenomas includes different types of tumors, each one having specific prognostic factors.

Perineural Involvement: What Does it Mean?

Perineural invasion is an important mechanism for local spread in certain malignant cutaneous neoplasms and is associated with aggressive tumor growth, increased frequency of recurrence, and increased morbidity and mortality. Thus, perineural invasion is often used both as a marker of malignancy and an indicator of aggressive behavior. There exists, however, a limited number of cutaneous and noncutaneous benign neoplasms in addition to reactive lesions that either demonstrates perineural involvement or mimics it. Given the association of the term "invasion" with malignant neoplasms, we use the term "perineural involvement" to describe neoplastic cells of any type infiltrating within nerves. Despite the presence of perineural involvement in these benign lesions, there is no evidence of aggressive behavior compared with similar examples which do not demonstrate perineural involvement. The aim of this article is to review cutaneous and noncutaneous benign neoplasms and reactive conditions that may demonstrate or mimic perineural involvement. Recognition of the spectrum of benign processes that may resemble perineural involvement may help prevent diagnostic confusion, misdiagnosis, and overly aggressive treatment.

Cyclin D1 Homogeneous Staining Regions by Fluorescent In Situ Hybridization: A Possible Indicator of Aggressive Behavior in Melanomas

Cyclin D1 Homogeneous Staining Regions by Fluorescent In Situ Hybridization: A Possible Indicator of Aggressive Behavior in Melanomas

Role of Ki-67 proliferation index and p53 expression in predicting progression of pituitary adenomas

Pituitary adenomas sometimes progress after surgery and can be locally invasive. Ki-67 and p53 expression are referred to as indicators of aggressive behavior in the World Health Organization Classification of Endocrine Tumors. The real value of these markers including an appropriate threshold for Ki-67 labeling index correlating with tumor progression is controversial. We identified 24 consecutive pituitary adenomas from patients who required surgery for recurrence within 5 years of their first procedure and 31 consecutive adenomas with no evidence of postsurgical progression within 5 years of first surgery. Case selection was based upon availability of complete clinical information, blocks, and slides for study. Immunohistochemistry for Ki-67 revealed that the tumors without progression had a proliferation index of 0.41% +/- 0.01% (mean +/- SEM) (n = 31) (range, 0.08%-1.2%) and the first biopsy from those tumors which progressed had a mean proliferation index of 1.45% +/- 0.09% (mean +/- SEM) (n = 24) (range, 0.1%-10.6%) (P = .01). With the use of ROC analysis, a threshold level of Ki-67 expression greater than 1.3% predicts progression with a high specificity. The group with progression had a higher proportion of nonfunctioning tumors (P < .005, chi(2)). There was no significant difference between the 2 groups with regard to invasion, suprasellar extension, size, tumor type, postoperative radiotherapy, extent of resection, sex, and age. Ki-67 labeling index was an independent predictor of progression (multivariate analysis, P < .011). p53 was positive in 12.5% of cases with surgical progression and in 9.6% of cases without progression, but the difference was not significant (P = .7; chi(2), 0.11).

Lymphovascular Invasion, as a Prognostic Marker in Patients with Invasive Breast Cancer

Purpose: The markers of prognosis are used to predict the clinical course of disease and the outcome for patients with invasive breast cancer. Our aim is to investigate the relationship of peritumoural lymphovascular invasion (LVI) with well-known prognostic markers.Patients and Methods: Eighty-one surgically treated patients with invasive breast cancer were evaluated in this study during a mean follow-up period of 46 months (12–72). The patient’s age (menopausal status), tumour size, nuclear grade, axillary lymph node involvement, and hormone receptor status were determined as markers of the prognosis. The relationship of LVI with these markers was established.Results: Except for menopausal status (p = 0.25) a close relationship was found between the presence of LVI and studied prognostic factors. LVI was positive in 29% of T1, 54% of T2 (p = 0.028) and 100% of T3 tumours (p = 0.002). The rate of LVI (+) has increased gradually as 0%, 38% and 77% (p = 0.001) with grades 1, 2 and 3 respectively. Positive LVI has been determined in 85% (p < 0.0001) and 73% (p = 0.0004) of oestrogen and progesterone receptor negative tumours respectively. LVI was present in 14% and 73% (p < 0.0001) of patients with negative and positive axilla respectively. Metastatic cancer caused mortality in seven patients of whom 86% had more than four involved axillary nodes, and 100% LVI (+).Conclusion: The high rate of positive LVI shows a close relationship with known markers of poor prognosis. The presence of LVI can predict a worse outcome for patients with invasive breast cancer. LVI may be used as an indicator of aggressive behaviour, metastatic ability (nodal and systemic) of the primary malignancy.

Application of the crypt isolation technique to the flow cytometric analysis of DNA content in gastric carcinoma

An inevitable limitation of conventional flow cytometric analysis of gastric cancer DNA content is that the preparations of tumor cell nuclei are contaminated with stromal cell nuclei. Using the crypt isolation technique, we separated tumor tissues from stromal tissues and analyzed the DNA content in samples of pure gastric cancer cells (64 intestinal-type and 46 diffuse-type) by flow cytometry. Morphologically, crypts from well-differentiated and moderately differentiated adenocarcinomas usually showed large tube-like or sheet structures, whereas tumor tissues isolated from poorly differentiated adenocarcinomas usually exhibited small tumor cell clumps or clusters of varying sizes. Tumor ploidy was divided into diploid, aneuploid, and multiploid subgroups. Aneuploidy and multiploidy were observed in 12% (13 of 110) and 64% (71 of 110) of gastric cancers, respectively. A high frequency of DNA aneuploidy or multiploidy was associated with intestinal-type tumors, but not with any of the other clinicopathologic variables tested. In contrast, high S-phase fraction values demonstrated a close association with tumors with abnormal ploidy, advanced stage, intestinal type, and late TNM stage. Our results suggest that S-phase fraction may be a more useful indicator of aggressive behavior in gastric cancers than DNA aneuploidy. To our knowledge, the present study is the first to report flow cytometric DNA content in a large number of gastric cancer samples obtained using the crypt isolation technique.

Endothelin-1, Endothelin-A- and Endothelin-B-receptor expression in preinvasive and invasive breast disease

Endothelin-1 (ET-1) is overexpressed in breast carcinomas and influences via its receptors (ETAR and ETBR) transformation, differentiation and growth processes in the human breast, but little is known about the ET expression in breast cancer precursors. On this basis we evaluated the expression of ET-1, ETAR and ETBR in a series of breast carcinomas, ductal (DCIS) and lobular carcinoma in situ (LCIS) and normal breast tissue by immunohistochemical (IH) methods. IH staining of ET-1, ETAR and ETBR was performed in 88 invasive breast carcinomas, with adjacent carcinoma in situ and concomitant normal breast tissue. Moderate or strong cytoplasmic immunostaining was observed for ET-1 in 33.3%, for ETAR in 45.3% and for ETBR in 55.7% of invasive breast carcinomas. Comparative analysis of invasive cancer (CA), concomitant carcinoma in situ (CIS) and normal breast epithelium (NBE) revealed a stepwise increase of ET-1 and ETAR expression in the sequence NBE < CIS < CA. ETBR expression tended to be slightly higher in CIS than in CA (NBE versus CIS and NBE versus CA, for ETAR and ETBR, p<0.001, respectively; NBE versus CA for ET-1, p=0.035). Our data suggest that the expression of ET-1, ETAR and ETBR correlates with the acquisition of malignant potential and may be used as a prognostic indicator of aggressive behaviour and invasive potential of premalignant breast lesions.

The use of novel stimuli as indicators of aggressive behavior in dogs.

To test the predictive value of a doll and an artificial hand, reactions of dogs (n=100) were compared to histories of behavior toward children. Each dog's reaction to the doll and the hand was categorized as normal, fearful, fearfully aggressive, or offensively aggressive. Sixty-five percent (n=37) of the dogs that had a normal or no reaction to the stimuli had a history of being good with children. Eighty-eight percent (n=34) of the dogs that had an aggressive reaction to the doll had a history of aggressive behavior toward a child. Dogs that were fearfully aggressive were significantly more likely to show fearful responses to the doll, and dogs that were either dominant or fearfully aggressive were more likely to exhibit aggression of the same type to the hand. The results of this study indicate that the doll and, to a lesser extent, the hand may be useful components in determining the aggressive tendencies of dogs. The results also point out the major limitations, because the false positives and false negatives are too frequent.

Significance of trisomy 7 related to PCNA index in cholesteatoma

Purpose: To define the biological features of the variable aggressiveness of cholesteatoma, we performed a retrospective cytogenetic and kinetic study. Materials and Methods: We studied 44 cases of cholesteatoma by using fluorescence in situ hybridization with specific a-satellite DNA probes for chromosome 7 and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) with PC10 monoclonal antibody. Results: We observed the presence of three or four chromosome 7 in elevated percentages of cells of 23 cases, and a high PCNA index (above mean value) in 22 cases. Conclusion: Based on our results, we maintain that the presence of an extra chromosome 7 is correlated with an increase of proliferative activity. Therefore, trisomy 7 can be considered a prognostic marker and an indicator of aggressive behavior in cholesteatoma.