Independent Regulation Research Articles

Slow-fast dynamics in non-linear enzyme cascades gives rise to spatial multiscaling.

Slow-fast dynamics in a well-mixed biological system is known to be associated with functional modularity with temporal hierarchy of processes. Yet, the significance of slow-fast dynamics in spatially distributed reaction-diffusion systems remains to be clarified.We assessed such systems with slow-fast dynamics, such as blood coagulation and predator-prey models to identify spatial patterns with independent parametric regulation, and performed their reaction-rate based sensitivity analysis with the help of computational one-dimensional reaction-diffusion models.In blood coagulation, spatial distribution of fibrin had two scales: a larger region with a high and almost constant fibrin concentration (corresponding to solid blood clot) and a narrow transition zone, where fibrin concentration decayed to zero (semi-liquid clot). Slow variables (e.g., the concentration of the coagulation factor IXa) regulated the clot size (large scale), while fast variables (e.g., concentrations of thrombin and factor Xa) regulated the thickness of the gel-to-liquid transition zone (small scale). Analysis of the simplified model of blood coagulation, composed only of factor X and IX activation, and of the predator-prey model confirmed this finding to be sufficiently general, and revealed that the limitation of fast enzyme production (due to precursor depletion or to kinetics with saturation) was crucial for this multi-scaling. Independent regulation of spatial scales also required species with slow kinetics to be upstream from species with fast kinetics.The shared slow-fast dynamics of the examined systems endow them with the ability to form spatial patterns, representing a new mechanism complementing the Turing-type and the Positional Information type pattern formation.

Exploring the role of N-acetyltransferases in diseases: a focus on N-acetyltransferase 9 in neurodegeneration.

Acetyltransferases, required to transfer an acetyl group on protein are highly conserved proteins that play a crucial role in development and disease. Protein acetylation is a common post-translational modification pivotal to basic cellular processes. Close to 80%-90% of proteins are acetylated during translation, which is an irreversible process that affects protein structure, function, life, and localization. In this review, we have discussed the various N-acetyltransferases present in humans, their function, and how they might play a role in diseases. Furthermore, we have focused on N-acetyltransferase 9 and its role in microtubule stability. We have shed light on how N-acetyltransferase 9 and acetylation of proteins can potentially play a role in neurodegenerative diseases. We have specifically discussed the N-acetyltransferase 9-acetylation independent function and regulation of c-Jun N-terminal kinase signaling and microtubule stability during development and neurodegeneration.

Physiological regulation of oral saliva ion composition and flow rate are not coupled in healthy humans-Partial revision of our current knowledge required.

Appropriate composition of oral saliva is essential for a healthy milieu that protects mucosa and teeth. Only few studies, with small sample numbers, investigated physiological saliva ion composition in humans. We determined saliva ion composition in a sufficiently large cohort of healthy adults and analyzed the effect of physiological stimulation. We collected saliva from 102 adults under non-stimulated and physiologically stimulated conditions (chewing). Individual flow rates, pH, osmolality, Na+, K+, Cl-, and HCO3- concentrations under both conditions as well as the individual changes due to stimulation (Δvalues) were determined. Non-stimulated saliva was hypoosmolal and acidic. Na+, Cl-, and HCO3- concentrations remained well below physiological plasma values, whereas K+ concentrations exceeded plasma values more than twofold. Stimulation resulted in a doubling of flow rates and substantial increases in pH, HCO3-, and Na+ concentrations. Overall, stimulation did not considerably affect osmolality nor K+ or Cl- concentrations of saliva. An in-depth analysis of stimulation effects, using individual Δvalues, showed no correlation of Δflow rate with Δion concentrations, indicating independent regulation of acinar volume and ductal ion transport. Stimulation-induced Δ[Na+] correlated with Δ[HCO3-] and Δ[Cl-] but not with Δ[K+], indicating common regulation of ductal Na+, Cl-, and HCO3- transport. We present a robust data set of human oral saliva ion composition in healthy adults and functional insights into physiological stimulation. Our data show (i) that flow-dependence exists for Na+ and HCO3- but not for K+ and Cl- concentrations, (ii) osmolality is flow-independent, (iii) regulation of Na+, Cl-, and HCO3- transport is coupled, (iv) regulation of flow rate and ion concentrations are independent and (v) spatially separated between acini and ducts, respectively.

Loss-of-function of RNA-binding protein PRRC2B causes translational defects and congenital cardiovascular malformation.

Alternative splicing generates variant forms of proteins for a given gene and accounts for functional redundancy or diversification. A novel RNA-binding protein, Pro-rich Coiled-coil Containing Protein 2B (PRRC2B), has been reported by multiple laboratories to mediate uORF-dependent and independent regulation of translation initiation required for cell cycle progression and proliferation. We identified two alternative spliced isoforms in human and mouse hearts and HEK293T cells, full-length (FL) and exon 16-excluded isoform ΔE16. A congenital heart disease-associated human mutation-mimicry knock-in of the equivalent variant in the mouse genome leads to the depletion of the full-length Prrc2b mRNA but not the alternative spliced truncated form ΔE16, does not cause any apparent structural or functional disorders. In contrast, global genetic inactivation of the PRRC2B gene in the mouse genome, nullifying both mRNA isoforms, caused patent ductus arteriosus (PDA) and neonatal lethality in mice. Bulk and single nucleus transcriptome profiling analyses of embryonic mouse hearts demonstrated a significant overall downregulation of multiple smooth muscle-specific genes in Prrc2b mutant mice resulting from reduced smooth muscle cell number. Integrated analysis of proteomic changes in Prrc2b null mouse embryonic hearts and polysome-seq and RNA-seq multi-omics analysis in human HEK293T cells uncover conserved PRRC2B-regulated target mRNAs that encode essential factors required for cardiac and vascular development. Our findings reveal the connection between alternative splicing regulation of PRRC2B, PRRC2B-mediated translational control, and congenital cardiovascular development and disorder. This study may shed light on the significance of PRRC2B in human cardiovascular disease diagnosis and treatment.

GLOBAL EXPERIENCE OF DEVELOPING COUNTRIES IN FORMING INNOVATIVE MECHANISMS OF GREEN ECONOMIC GROWTH

The article examines the international experience of developing countries in creating and implementing innovative mechanisms to achieve green economic growth. The necessity of implementing such mechanisms to ensure sustainable economic development is substantiated. It is argued that innovative approaches are key to preserving the ecological balance and improving the well-being of the population. It was established that the effective use of natural resources requires the integration of environmental and economic strategies. Based on the set goal, various models and practices of green growth applied in developing countries are considered. Successful examples are studied and found that their adaptation can contribute to global sustainable development. Further perspectives of research should be aimed at studying the possibilities of scaling and optimization of these innovative mechanisms in the context of global challenges. It was determined that each country has its own characteristics due to the level of development of the country, its geographical location, the presence of natural resources and the specifics of the concepts, models and development tools used, which correspond to national priorities and accumulated experience, and therefore the transition to a "green" economy for different states will take place under different scenarios. It illustrates the benefits and challenges of implementing green innovation for developing countries using the example of China and its chart of China's key industrial strategies for green manufacturing. During the research of this topic, it was found that the consistent and effective implementation of the principles of "green" investment, as well as "green" development in general, is possible only in a regulatory environment, transparent and fair, developed in accordance with the principle of the rule of law, with independent courts procedures, with the participation of independent market regulators acting in accordance with national interests, respecting the rights of investors and consumers. On the other hand, for their principles of implementation a vital role is played by socially responsible business behavior, one of the key indicators of which is the attitude of business to environmental issues, the efficiency of resource use and energy efficiency, and the disclosure of socially significant non-financial information. It is noted that in Ukraine climate and investment policies function separately from each other, and sometimes their goals are mutually contradictory, which prevents or slows down investments in the development of "green" infrastructure.

Visible-Near Infrared Independent Modulation of Hexagonal WO3 Induced by Ionic Insertion Sequence and Cavity Characteristics.

Dual-band electrochromic materials have attracted significant attention due to their ability to independently control sunlight and solar heat. However, these materials generally exhibit notable limitations, and the mechanisms for their dual-band independent regulation remain poorly understood. Here, the visible-NIR-independent regulation capabilities of hexagonal WO3 (h-WO3) are introduced for the first time. A structure-activity relationship that perfectlylinks the microscopic ion insertion sequence and cavity characteristics to the macroscopic dual-band electrochromic properties is established. The progressive ion intercalation process and the distinctive optical activity of the cavities are keys for enabling h-WO3 to independently modulate "bright," "cool," and "dark" modes. Notably, h-WO3 demonstrates superior dual-band electrochromic performance with a broadband full shielding effect from 550 to 2000nm, achieving the widest full shielding band in dual-band electrochromic studies. Additionally, h-WO3 shows a high discharge capacity of 270.9 mAh m- 2 at 0.25 A m- 2, and requires only 49.1 and 209.7 mAh m- 2 to complete a full round-trip switch between "bright-cool" and "bright-dark" modes, respectively. The constructed device offers a dynamic temperature control range of up to 10.5°C and supports a maximum voltage of 2.86V, underscoring its considerable potential for practical applications and energy efficiency.

A Comparative Analysis of the Design and Implementation of the Twin Peaks Model of Financial Regulation in South Africa and Australia

Abstract In response to the 2007-2009 global financial crisis, South Africa, like most other countries, embarked on a journey of financial regulatory reform. This transformation has seen the country shift away from a silo approach, where various sectoral regulators supervise different financial institutions, towards the Twin Peaks model of financial regulation. The term “Twin Peaks” refers to the separation of regulatory functions between two independent regulators. For instance, one regulatory agency is responsible for the supervision of the safety and soundness of financial institutions, and the other is focussed on the regulation of business conduct. The Twin Peaks model is designed to provide the benefits and efficiencies of an integrated approach while simultaneously addressing inherent conflicts between the objectives of the safety and soundness of the financial system and consumer protection. The Financial Sector Regulation Act provides for the Twin Peaks model in South Africa. This Act positions South Africa as one of the first developing countries to adopt the model. On the other hand, Australia is the pioneer of the model since it implemented it in 1998. Its implementation in Australia, much as in South Africa, involved the separation of regulators for prudential soundness on the one hand and market conduct and consumer protection on the other. In this article, the authors provide a comparative analysis of the design and implementation of the Twin Peaks model in Australia and South Africa. They argue that while there are minor differences between the design and implementation of this model in Australia and South Africa, the model was customised and implemented to align with the specific needs of each country

Construction of a photovoltaic low-voltage grid connected power regulation system based on partition control

Abstract Photovoltaic power generation is a green and renewable power generation mode and has been widely used. However, the power fluctuation of photovoltaic power generation has a significant impact on the power grid, which is very important. Based on the concept of regional control, this paper studies the photovoltaic low-voltage grid-connected power regulation system. The photovoltaic power generation system is divided into multiple regions, and each region is provided with an independent control unit and regulation strategy under the control of multiple control areas. The regional factors are combined, such as light intensity and power voltage. In the case of the city, the impact of the area on the whole is dynamically adjusted to achieve the overall balance of power and the stability of the system. The experimental results show that the photovoltaic low-voltage grid-connected power regulation system constructed by regional control performs well and has a high degree of stability. According to the change in light intensity and load demand, the system can achieve accurate control and distribution of photovoltaic power generation.

From state financial control to modern audit: evolutionary development in Russia and other countries

The article is devoted to the study of the evolution of state financial control and audit. The historical facts of their origin in the Great Roman Empire, China, India, Great Britain, the USA and Russia are given. Four stages of audit development in modern Russia are identified and a fairly detailed description of each stage is given. The necessity of modernizing the process of self-regulation of the auditing profession is substantiated, taking into account the recommendations of the International Forum of Independent Regulators of Auditing, the development and effective implementation of a new Concept and a new Strategy of Russian audit, the implementation of which will create conditions for the consolidation of the audit professional community, contributing to the development and functioning of mechanisms for involving the audit community in the processes of solving strategic tasks aimed at improving the efficiency of functioning modern models of state financial control and audit, the development of the national economy and ensuring the economic security of the country. Special attention is paid to taking measures to increase the level of demand and value of the audit.

Notch-Dependent Expression of the Drosophila Hey Gene Is Supported by a Pair of Enhancers with Overlapping Activities.

Drosophila Hey is a basic helix-loop-helix-orange (bHLH-O) protein with an important role in the establishment of distinct identities of postmitotic cells. We have previously identified Hey as a transcriptional target and effector of Notch signalling during the asymmetric division of neuronal progenitors, generating neurons of two types, and we have shown that Notch-dependent expression of Hey also marks a subpopulation of the newborn enteroendocrine (EE) cells in the midgut primordium of the embryo. Here, we investigate the transcriptional regulation of Hey in neuronal and intestinal tissues. We isolated two genomic regions upstream of the promoter (HeyUP) and in the second intron (HeyIN2) of the Hey gene, based on the presence of binding motifs for Su(H), the transcription factor that mediates Notch activity. We found that both regions can direct the overlapping expression patterns of reporter transgenes recapitulating endogenous Hey expression. Moreover, we showed that while HeyIN2 represents a Notch-dependent enhancer, HeyUP confers both Notch-dependent and independent transcriptional regulation. We induced mutations that removed the Su(H) binding motifs in either region and then studied the enhancer functionality in the respective Hey mutant lines. Our results provide direct evidence that although both enhancers support Notch-dependent regulation of the Hey gene, their role is redundant, as a Hey loss-of-function lethal phenotype is observed only after deletion of all their Su(H) binding motifs by CRISPR/Cas9.

THYLAKOID FORMATION 1 interacts with FLOWERING LOCUS T and modulates temperature-responsive flowering inArabidopsis.

The intracellular localization of the florigen FLOWERING LOCUS T (FT) is important for its long-distance transport toward the shoot apical meristem. However, the mechanisms regulating the FT localization remain poorly understood. Here, we discovered that in Arabidopsis thaliana, the chloroplast-localized protein THYLAKOID FORMATION 1 (THF1) physically interacts with FT, sequestering FT in the outer chloroplast envelope. Loss of THF1 function led to temperature-insensitive flowering, resulting in early flowering, especially under low ambient temperatures. THF1 mainly acts in the leaf vasculature and shoot apex to prevent flowering. Mutation of CONSTANS or FT completely suppressed the early flowering of thf1-1 mutants. FT and THF1 interact via their anion binding pocket and coiled-coil domain (CCD), respectively. Deletion of the CCD in THF1 by gene editing caused temperature-insensitive early flowering similar to that observed in the thf1-1 mutant. FT levels in the outer chloroplast envelope decreased in the thf1-1 mutant, suggesting that THF1 is important for sequestering FT. Furthermore, THF1 protein levels decreased in seedlings grown at high ambient temperature, suggesting an explanation for its role in plant responses to ambient temperature. A thf1-1 phosphatidylglycerolphosphate synthase 1 (pgp1) double mutant exhibited additive acceleration of flowering at 23 and 16°C, compared to the single mutants, indicating that THF1 and phosphatidylglycerol (PG) act as independent but synergistic regulators of temperature-responsive flowering. Collectively, our results provide an understanding of the genetic pathway involving THF1 and its role in temperature-responsive flowering and reveal a previously unappreciated additive interplay between THF1 and PG in temperature-responsive flowering.

Thermal Rectification in Graphene-Boron Nitride Nanotube Hybrid Structures: An Independent Control Mechanism for Forward and Backward Heat Flux.

The weak van der Waals interactions in the out-of-plane direction result in markedly low thermal conductivity in one-dimensional (1D) and two-dimensional (2D) materials, which substantially restricts their applications. Developing three-dimensional (3D) columnar hybrid structures, featuring high thermal conductivity both within and beyond the plane, effectively addresses this challenge. This study investigated a 3D hybrid structure composed of graphene and boron nitride nanotubes (GR-BNNTs) using non-equilibrium molecular dynamics simulations. This approach allowed the examination of the formation mechanisms and key factors influencing thermal rectification (TR) in these materials. Our findings reveal a novel mechanism for independently regulating forward and backward heat fluxes in GR-BNNTs. By manipulating the thermal properties of the BNNTs and the graphene layer, the TR ratio can be controlled flexibly. Additionally, we identify specific strategies for independently adjusting the heat flux, such as altering the intercolumn distance of BNNTs, which impacts the backward flux merely, while applying strain to affect the forward flux merely. This research introduces a novel concept of independent regulation of forward and backward heat fluxes, providing significant insights into phonon thermal transport in 3D hybrid structures.

Assessing regulatory governance for the water and sanitation Agencies in Brazil

This paper focuses on evaluating and benchmarking the governance of regulatory agencies in Brazil's water and sanitation sector. Using a unique database obtained through the application of a questionnaire, we developed an index of regulatory governance and ranked all the agencies in Brazil that participated in the study. The index is an aggregate number of the evaluation of four key governance characteristics: Autonomy, Transparency, Accountability, and Tools & Capacity, including not only formal aspects of regulation but also indicators related to actual implementation. Based on 18 different indices, we analyze the positions of agencies with regard to different aspects of their regulatory governance, considering not only performance in each variable but also scores in the different components of each category. This evaluation identifies particular agency shortcomings regarding governance and indicates needed improvements. Although Brazil has strived to consolidate the governance design of its regulatory agencies, implementing the independent regulator model still faces several challenges. Various aspects of governance can be improved to pursue greater efficiency and quality in providing services, particularly in Accountability and Political Autonomy, where the country shows the largest number of regulatory agencies with the lowest scores. Conversely, almost half of the participating agencies show optimal scores in the Regulatory Autonomy aspect of governance. The results, for the most part, corroborate the institutional theory, in which the role of institutions is paramount for greater efficiency in providing public services, as it reduces information asymmetries and transaction costs and maximizes economic incentives.

Recycling of Ships Act 2019: challenges and opportunities for ship recycling industry in India

ABSTRACT The Recycling of Ships Act 2019 (RSA 2019) was inspired by the Hong Kong Convention for the Safe and Environmentally Sound Recycling of Ships 2009, and it aimed at setting standards for ship recycling and laying down a statutory mechanism for enforcing such standards. This paper discusses the relevance and the contextual factors that led to the formulation of the RSA 2019. The RSA has invited sharp criticism, stressing the need to make appropriate amendments to the Act to ensure a safe and sound recycling environment and safeguard the health of recycling workers. An analysis of the recent trends in the ship recycling market has been carried out in light of implementing the RSA. The long-term sustainability of the ship recycling operations requires the formation of a collective association of the recycling nations in South Asia, even though each nation may adopt independent regulations of its own.

A tendon-driven actuator with cantilever initiated variable stiffness used for robotic fingers

Variable stiffness actuators (VSAs) have emerged as a key actuation technology known for their bionic performance and task adaptability. However, current VSAs often exhibit relatively large sizes, making them possible for use in robotic arms and legs but less convenient for integrations into robotic hands. This paper introduces a compact design of a tendon-driven variable stiffness actuator (TVSA) based on an adjustable cantilever mechanism, which can be embedded into a robotic finger. This implementation endows the robotic finger with the independent regulation of joint position and stiffness. A concise and computationally efficient stiffness mapping model from the TVSA to the finger joints is then established, providing a theoretical foundation for the stiffness regulation of the tendon-driven fingers. A prototype of a robotic hand equipped with the presented TVSA demonstrates safe interactions with various objects of diverse shapes, weights and stiffness.

Hepatocyte-specific loss of DDB1 attenuates hepatic steatosis but aggravates liver inflammation and fibrosis in MASH.

MASH is a common clinical disease that can lead to advanced liver conditions, but no approved pharmacotherapies are available due to an incomplete understanding of its pathogenesis. Damaged DNA binding protein 1 (DDB1) participates in lipid metabolism. Nevertheless, the function of DDB1 in MASH is unclear. Clinical liver samples were obtained from patients with MASH and control individuals by liver biopsy. Hepatocyte-specific Ddb1-knockout mice and liver Hmgb1 knockdown mice were fed with a methionine-and choline-deficient diet to induce MASH. We found that the expression of DDB1 in the liver was significantly decreased in MASH models. Hepatocyte-specific ablation of DDB1 markedly alleviated methionine-and choline-deficient diet-induced liver steatosis but unexpectedly exacerbated inflammation and fibrosis. Mechanistically, DDB1 deficiency attenuated hepatic steatosis by downregulating the expression of lipid synthesis and uptake genes. We identified high-mobility group box 1 as a key candidate target for DDB1-mediated liver injury. DDB1 deficiency upregulated the expression and extracellular release of high-mobility group box 1, which further increased macrophage infiltration and activated HSCs, ultimately leading to the exacerbation of liver inflammation and fibrosis. These data demonstrate the independent regulation of hepatic steatosis and injury in MASH. These findings have considerable clinical implications for the development of therapeutic strategies for MASH.

ОРГАНИ ДЕРЖАВНОЇ ВЛАДИ ЯК СУБ’ЄКТИ ВІЙСЬКОВО-ТЕХНІЧНОГО СПІВРОБІТНИЦТВА

The article analyzes the issues of competence of public authorities as subjects of legal relations in the field of military-technical cooperation. Based on the functional division of state power, the structure of state authorities in the field of military-technical cooperation is considered. It is stated that the only legislative body in Ukraine is the Parliament, the Verkhovna Rada of Ukraine. The relevant government bodies, primarily the Cabinet of Ministers of Ukraine, represent the executive branch of government. Based on the functional approach, the author proposes a three-tier system of central executive bodies in the field of military-technical cooperation, which includes: Level I – the Cabinet of Ministers of Ukraine; Level II – ministries of Ukraine (Ministry of Defense, Ministry of Strategic Industries, Ministry of Finance); Level III – state services, state inspections, state agencies, independent regulators and bodies with special status (State Export Control Service, State Space Agency). It was noted that an advisory body should be established with the competence to deal with military-technical cooperation, namely: The Interagency Commission on Military-Technical Cooperation and Export Control Policy, which is an advisory body to the President of Ukraine. Key words: military-technical cooperation, international cooperation, subjects of legal relations, public authorities, military-industrial complex.

Template Evolution Induced Relay Self-Assembly for Mesoporous Carbonaceous Materials via Hydrothermal Carbonization.

Constructing carbonaceous materials with versatile surface structures still remains a great challenge due to limited self-assembly methods, especially at high temperatures. This study presents an innovative template evolution induced relay self-assembly (TEIRSA) for the fabrication of large polyoxometalate (POM)-mixed carbonaceous nanosheets featuring surface mesoporous structures through hydrothermal carbonization (HTC). The method employs POM and acetone as additives, cleverly modulating the Ostwald ripening-like process of P123-based micelles, effectively addressing the instability challenges inherent in traditional soft-template methods, especially within the demanding carbohydrate HTC process. Additionally, this method allows for the independent regulation of surface architectures through the selection of organic additives. The resulting nanosheets exhibit diverse surface morphologies, including surface spherical mesopores, 1D open channels, and smooth surfaces. Their unexpectedly versatile properties have swiftly garnered recognition, showing potential in the application of lithium-sulfur batteries.

Dual-functional tunable emitter with high visual transparency for energy saving and information encryption

Achieving thermal radiation regulation is attractive in the field of thermal management and information security. However, maintaining high visual transparency simultaneously is a challenging task, but it holds vital importance for applications such as smart windows and multispectral information encryption. Here, we designed and fabricated a multilayer film based on thermochromic material vanadium dioxide (VO2) with high transmittance (62.1 %) in visible region and excellent emissivity regulation (0.3) in mid-infrared region. Besides, quantities of energy saving simulations among 85 administrative districts in the United States and China and outdoor tests in Shanghai were done to verify the performance in different climates for the application of energy saving smart window. Furthermore, by taking advantage of its high transmittance in visible band and emissivity tunability in mid-infrared band, VO2-based multilayer film has demonstrated the enormous potential application for information encryption through a proof-of-concept validation. By presenting the novel strategy to achieve independent multispectral regulation, this work offers a solution for multidisciplinary fields including green buildings, adaptive multi-scenes camouflage and other information security.

Analysis and design of high‐frequency multiple‐output wireless power transfer system with load‐independent class‐E/F inverter

AbstractThis paper proposes a high‐frequency multiple‐receiver wireless power transfer (WPT) system with a load‐independent class‐E/F inverter. Each receiver has a post‐regulator, which changes the equivalent resistance seen from the inverter to obtain the necessary power for output voltage regulation. Because the load‐independent class‐E/F inverter generates constant AC current ( ), the transmitter supplies the minimum required power to the receivers by the change of the equivalent resistances. Besides, the load‐independent inverter consistently achieves zero‐voltage switching (ZVS) without any control. As a result, no information feedback by wireless communication is necessary for output regulation and ZVS achievement, simplifying the system configuration and improving the transient response of the control. This paper presents analytical expressions of the proposed system. Besides, the experiment was carried out with a two‐receiver WPT system. The implemented system worked well by individual and independent output regulation of the post regulator at each receiver. The implemented WPT system achieved the power‐delivery efficiency of 83.4% at the 6.78 MHz transmission frequency and the total output power of 40 W.