Increase In Protein Content Research Articles

Pea and soy protein isolate fractal gels: The role of protein composition, structure and solubility on their gelation behaviour

The gelation behaviour of two different pea protein isolates and one soy protein isolate were investigated with a focus on the role of the protein properties. Protein solubility was the lowest in pH 3 citrate-phosphate buffer (<10% w/w), increased in pH 7.4 phosphate-buffered saline (12–21% w/w), and was the highest in pH 7.6 MilliQ water (∼20–40% w/w). Heat-induced gelation conditions for the protein sources were sensitive to both the soluble and the insoluble fractions as obtained during extraction. At low protein concentrations (≤5% w/v), the proteins started to lose their viscoelastic behaviour and exhibited predominantly viscous properties. Fitting of the fractional Kelvin-Voigt model to the frequency sweeps showed an increase in the fractal gel strength with increasing protein concentration. Secondary structures of the soluble species showed mostly unordered proteins, suggesting that the proteins were denatured during the commercial extraction process although gelation has to date been suggested to be highly dependent on the denaturation of soluble proteins. Synchrotron Radiation Circular Dichroism measurements of the insoluble proteins showed a significant amount of ordered protein structures. SEM imaging of the gels also suggested a new gelation pathway in which insoluble proteins act as dispersed fillers within a continuous matrix of soluble proteins. This research elucidated the role of different protein fractions, globulins and albumins, and their secondary structure in the formation of a gel network and how this affects their viscoelastic behaviour.

Effects of dietary supplementation of polysaccharide from Agaricus blazei Murr on productive performance, egg quality, blood metabolites, intestinal morphology and microbiota of Korean quail.

This study aimed to investigate the effects of dietary supplementation with Agaricus blazei polysaccharide (ABP) at varying concentrations on the performance, egg quality, blood biochemistry, intestinal morphology, and microflora of quail. The study involved a total of 2,700 Korean quails, which were randomly divided into three groups. The measured variables encompassed productive performance, egg parameters, carcass parameters, serum metabolites, immune response parameters, antioxidative properties, and gut microbiome. The addition of ABP did not have a significant effect on average daily feed intake. However, it was found to increase the average daily egg weight and egg production rate, reduce the feed-egg ratio. There were no significant impacts on egg quality measures such as egg shape index, egg yolk index and color, egg yolk and protein content. However, ABP supplementation significantly increased the Hough unit (p<0.01) and decreased the rate of unqualified eggs (p<0.01). Regarding serum parameters, the inclusion led to an increase in total protein concentration (p<0.05) and a reduction in low-density lipoprotein cholesterol (p<0.05). There were no significant effects observed on immune indicators such as immunoglobulin A (IgA) and IgM. ABP supplementation increased the levels of serum antioxidant indicators, including glutathione peroxidase, total superoxide dismutase (p<0.05), and total antioxidant capacity colorimeter (p<0.05). Furthermore, ABP supplementation significantly elevated the intramuscular fatty acid content in quail meat. Additionally, ABP supplementation demonstrated a significant improvement in the diversity of gut microbiota and induced alterations in the composition of the gut microbiota. The findings of this study indicate that dietary supplementation of ABP enhanced production performance and antioxidant capacity while increasing the levels of polyunsaturated fatty acids in quail muscle.

Cardioprotective Potential of Cymbopogon citratus Essential Oil against Isoproterenol-induced Cardiomyocyte Hypertrophy: Possible Involvement of NLRP3 Inflammasome and Oxidative Phosphorylation Complex Subunits.

Cymbopogon citratus (DC.) Stapf is a medicinal and edible herb that is widely used for the treatment of gastric, nervous and hypertensive disorders. In this study, we investigated the cardioprotective effects and mechanisms of the essential oil, the main active ingredient of Cymbopogon citratus, on isoproterenol (ISO)-induced cardiomyocyte hypertrophy. The compositions of Cymbopogon citratus essential oil (CCEO) were determined by gas chromatography-mass spectrometry. Cardiomyocytes were pretreated with 16.9 µg/L CCEO for 1 h followed by 10 µmol/L ISO for 24 h. Cardiac hypertrophy-related indicators and NLRP3 inflammasome expression were evaluated. Subsequently, transcriptome sequencing (RNA-seq) and target verification were used to further explore the underlying mechanism. Our results showed that the CCEO mainly included citronellal (45.66%), geraniol (23.32%), and citronellol (10.37%). CCEO inhibited ISO-induced increases in cell surface area and protein content, as well as the upregulation of fetal gene expression. Moreover, CCEO inhibited ISO-induced NLRP3 inflammasome expression, as evidenced by decreased lactate dehydrogenase content and downregulated mRNA levels of NLRP3, ASC, CASP1, GSDMD, and IL-1β, as well as reduced protein levels of NLRP3, ASC, pro-caspase-1, caspase-1 (p20), GSDMD-FL, GSDMD-N, and pro-IL-1β. The RNA-seq results showed that CCEO inhibited the increase in the mRNA levels of 26 oxidative phosphorylation complex subunits in ISO-treated cardiomyocytes. Our further experiments confirmed that CCEO suppressed ISO-induced upregulation of mt-Nd1, Sdhd, mt-Cytb, Uqcrq, and mt-Atp6 but had no obvious effects on mt-Col expression. CCEO inhibits ISO-induced cardiomyocyte hypertrophy through the suppression of NLRP3 inflammasome expression and the regulation of several oxidative phosphorylation complex subunits.

Effects of tilapia bone gelatin addition in broccoli jelly candy towards the protein, fat, fiber, and vitamin C content

This study aims to evaluate the effect of tilapia bone gelatin addition to the formulation of broccoli jelly candy and determine the best formulation from its protein, fat, fiber, and vitamin C content. The results show that tilapia bone gelatin addition to broccoli jelly candy significantly increases protein content from 8.17 - 12.19%, due to the gelatin composition that mostly consists of protein. This addition also increases the vitamin C content from 7.26 - 15.18 mg/g, due to gelatin’s ability to improve the thermal stability of ascorbic acid. However, the addition of gelatin does not affect the fat and fiber content. The best formulation based on protein content is Formulation 6 (13g of gelatin), meanwhile, formulation 4 (11g of gelatin) has the best formulation for vitamin C content.

Formulation of Chicken Nuggets Supplemented with Mutton and Fish Livers: Insights from Antioxidant and Textural Studies.

The use of byproducts from the food industry and the investigation of substitute sources are becoming progressively significant in fulfilling the consumer demand for animal-based protein. This study aimed to investigate the nutritional value of mutton and fish livers and their future application as a source of high-added-value proteins for supplement formulation. We performed compositional analysis (moisture, ash, crude protein, crude fat), free fatty acid (FFA) analysis, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the color, peroxide value (POV), and total phenolic composition (TPC) were assessed to evaluate the nutritional value and shelf stability of mutton and fish livers. The optimized proximate and kinetics were later used to develop chicken nuggets with different percentages of mutton and fish liver added. The formulation was tested for the textural and organoleptic properties of value-added chicken nuggets that predict consumer acceptability. Comparative analysis of the variance between mutton and fish liver showed a highly significant (P<0.01) decrease in moisture, ash, protein, fat, DPPH, and TPC at different days and hours. The mutton liver had relatively high antioxidant potential (25.9% DPPH and 154-mg GAE/100 g TPC) compared with the fish liver. However, the fish liver's FFA and POV (2.4% for both) were higher than those of the mutton liver. The results showed that, after formulation, an increase in the amount of liver led to a highly significant (P<0.01) rise in the nutritional value of the nuggets, including a 1.5%∼2.0% increase in protein content. This research indicates that valuing mutton and fish liver as a protein replacer in processed foods can be useful in developing healthy food products.

Insights from morpho-physio-biochemical and molecular traits of hot pepper genotypes contrasting for heat tolerance

The present study compared the morpho-physiological, biochemical, and molecular responses of heattolerant (DLS-161-1) and heat-susceptible (DchBL-240) genotypes of hot pepper under high-temperature conditions. The results showed that DLS-161-1 performed significantly better for various morphological parameters such as plant height (60 cm), number of fruits per plant (180.33), average fruit length (8.40 cm) and leaf parameters. While DChBL-240 has significantly higher malformed seeds per fruit (15.06) and average fruit diameter (12.56 cm). The physiological responses also differed significantly between the two genotypes, with the DLS-161-1 exhibiting higher membrane stability index (65.99%), pollen viability (92.10%), photosynthetic rate (20.73 μmol CO2 m²/sec.), stomatal conductance (0.31 mol H2O m²/sec.), and transpiration rate (8.85 mmol H2O m²/sec). Biochemical analysis revealed significantly increased activity of antioxidant enzymes such as guaiacol peroxidase (564.99 U/g F.W) and catalase (711.90 U/g FW), increased protein content (13.38 mg/g F.W) and reduced malondialdehyde generation (13.42 nmol g-1 FW) in DLS-161-1 compared to heat susceptible genotype (DChBL-240). Further, two of the six heat shock protein genes, CaHSP2271 and CaHSP3, were highly upregulated in the DLS 161-1. The observations suggest that DLS-161-1 could adapt to high-temperature conditions by regulating its morphological, physiological, biochemical and molecular mechanisms.

Sulfation, characterization, antibacterial activity, and action mechanism of rice bran polysaccharides

In this study, the rice bran polysaccharide (RBP) was modified using the sulfur trioxide-pyridine complexation method. The effects of sulfation modification on the physicochemical properties, structure, bacterial inhibition, and inhibitory mechanism of the polysaccharide were investigated. Sulfated modification reduces the carbohydrate and protein content, increases the molecular weight, and changes the proportion of monosaccharides of RBP. UV–Vis, FT-IR, and NMR spectra confirmed that the modification was successful and the sulfation substitution occurred on hydroxyl groups at C-6. The sulfated rice bran polysaccharide (SRBP) inhibited all the five pathogenic bacterial strains, among which Staphylococcus aureus was the most sensitive, with an MIC of 4 mg/mL. Scanning electron microscopy images revealed that SRBP caused morphological damage and cell rupture of the bacterium, thereby causing an increase in nucleic acid and protein contents, conductivity, alkaline phosphatase, and Na+/K+-ATPase. Clearly, SRBP can act as an alternative derivative of natural antibacterial agents for industrial applications.

Boosting the resilience to drought of crop plants using wood distillate: A pilot study with lettuce (Lactuca sativa L.)

Increasingly severe drought driven by climate change is harming crop quality and productivity, making it crucial to find nature-based solutions like wood distillate (WD) to aid crops withstand these stressful conditions. This study investigated the effects of WD application on the growth and stress responses of lettuce (Lactuca sativa L.) under drought conditions. The experiment included periodic measurements of plant growth-related parameters and stress indices to evaluate the effectiveness of WD in mitigating drought-induced damage. The results showed a significant increase (13–26 %) in fresh biomass of the aboveground portion of lettuce treated with WD, indicating the potential of WD as a biostimulant to promote plant growth. In addition, treatment with WD led to a significant increase in total soluble protein content (14–28 %), showing a possible positive effect on protein synthesis. The enhancement of anti-radical activity (measured in terms of DPPH) following the application of WD up to 42 % reflected the ability of the plant to scavenge harmful reactive oxygen species and alleviate oxidative stress. The observed reduction (up to 19 % in comparison with control) in MDA content also confirmed the effectiveness of WD in protecting the integrity of plant cell membranes from oxidative damage. Despite the beneficial effects on anti-radical activity, the total content of the antioxidant compounds (phenols and flavonoids) decreased with the use of WD (5–14 %), most likely suggesting complex interactions between WD and the biosynthesis of these secondary metabolites. The study showed the positive effect of WD application on the growth and stress tolerance of lettuce plants under drought conditions. These results can provide new insights into sustainable agricultural practices and the potential application of WD as a nature-based and effective means to improve crop productivity, especially in water scarce areas.

Effect of addition of yeasts and enzymes during fermentation on physicochemical quality of fine aroma cocoa beans

Theobroma cacao is a recognized crop worldwide within the market for raw materials or processed products. Cocoa beans quality, industrial transformation and health benefits have been widely studied, but there are few studies on the fermentation process and even less on Ecuadorian cacao. In Latin America, this crop plays a very important role from an ecological and social viewpoint. Production is usually carried out by local farmers who also perform the natural fermentation of the beans, without specific management, which represents a challenge in terms of reliability and batch-to-batch homogeneity. This drawback can be effectively addressed by controlling the fermentation conditions. In addition, certain enzymes have the potential to improve the fermentation process. The aim of this work was to study the effect of using yeasts and/or enzymes during fermentation on the physicochemical quality characteristics of cocoa beans. Two trials were carried out, the first used yeasts (Saccharomyces cerevisiae), with and without molasses, and the second used enzymes (Polyphenoloxidase and Pectintranseliminase). During the fermentation process there was not external temperature control, although cocoa beans mass temperature was assessed. After the assay, the cutting test and physicochemical variables (percentage of testa, testa and cotyledon pH, moisture, ash, acidity, protein and fat contents) were evaluated. The yeasts trial stood out as the most favorable option, showing better fermentation in the cutting test, a slight increase in protein content and lower moisture. The inclusion of enzymes in the trials resulted in higher fermentation temperatures and fat content of the beans, but the samples showed lower fermentation percentage, higher moisture and mold presence, as enzymes make it difficult to completely dry the samples. It can be concluded that the addition of yeasts and/or enzymes influences fermentation and could improve the process in order to obtain high quality cocoa beans.

Pathways of inhibition of filamentous sludge bulking by slowly biodegradable organic compounds

The organic compound composition of wastewater, serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activated sludge. This study focused on the impact of typical soluble and slowly-biodegradable organic compounds, investigating the pathways through which these substrates affect the occurrence of filamentous bulking in systems operated under both high- and low-oxygen conditions. Results showed that slowly-biodegradable organic compounds lead to a concentrated distribution of microorganisms within flocs, with inward growth of filamentous bacteria. Both Tween-80 and granular starch treated systems exhibited a significant increase in protein content. The glucose system, utilizing soluble substrates, exhibited a markedly higher total polysaccharide content. Microbial communities in the Tween-80 and granular starch treated systems were characterized by a higher abundance of bacteria known to enhance sludge flocculation and settling, such as Competibacter, Xanthomonadaceae and Zoogloea. These findings are of high significance for controlling the operational performance and stability of activated sludge systems, deepening our understanding and providing a novel perspective for the improvement of wastewater treatment processes.

Perspective herbicides for post-sowing use in narrow-leafed lupin crops

Technical and economic efficiency of Pilot herbicide when applied on green leaf in narrow-leafed lupine crops was studied. The research was conducted in field and plot experiments according to the generally accepted methods in the southwestern part of the Non-Chernozem zone of Russia. The soil of the experimental field was gray forest light loamy in mechanical composition. The following application rates of the preparation were used: 1.5, 2.0 and 2.5 l/ha in the phase of appearance of two true leaves in lupine. The following plants showed high sensitivity even at the minimum application rate: cleavers (Galium aparine L.), wild buckwheat (Fallopia convolvulus L.), lamb's-quarters (Chenopodium album L.), one of the types of hemp nettle (Galeopsis sp. L.), shepherd's purse (Capsella bursa pastoris L. Medik), field violet (Viola arvensis Murr.), chickweed (Stellaria media L.). Application of GalactAlt graminicide (application rate 1.0 l/ha) after treatment of narrow-leafed lupine crops with Pilot herbicide promoted almost complete destruction of annual grass weeds. Application of Pilot herbicide had a favorable effect on nitrogen content in both dry aboveground mass and root mass of lupine. Reduction of weediness of crops when using Pilot contributed to the increase in lupine yield. In the variant with the application of 1.5 l/ha of the herbicide, the average yield of narrow-leafed lupine seeds exceeded the control by 0.53 t/ha in three years of the trials. The highest increase in seed yield of narrow-leafed lupine (0.96 t/ha) was observed in the variant that involved the application of herbicide Pilot at a concentration of 1.5 l/ha in the phase of one or two true leaves in lupine and herbicide GalactAlt at a concentration of 1.0 l/ha in the phase of lupine budding. Chemical analyses showed an increase in crude protein content in narrow-leafed lupine seeds by 2.3–4.2% when the crops were treated with Pilot herbicide compared to the control variant, where the treatment was not carried out.

Arbuscular mycorrhizal fungi impact on yield attributes, protein quantity and quality in bread wheat (Triticum aestivum L.) grown under drought stress

Drought is increasingly becoming a production constraint in wheat world-wide. Arbuscular mycorrhizal fungi (AMF)-bread wheat symbiosis has been shown to improve the tolerance under drought stress. The objective of this study was to determine the effects of AMF inoculation on yield attributes (spike number per plant, spike weight per plant, number of grains per spike, grain weight per spike and 1000 grain weight) and protein quantity and quality in two bread wheat cultivars (PAN3497 and SST806) grown under drought stress. Plant growth was significantly reduced due to drought stress in both cultivars. Shoot dry weight was significantly increased (23%) due to AMF inoculation in SST806. AMF inoculation caused a further significant increase of protein content in both cultivars (10.70% and 13.42% in PAN3497 and SST806, respectively). Low molecular weight (LMW) glutenin was significantly decreased in PAN3497 under drought stress. However, the monomeric proteins were significantly increased by drought and/or a combination of drought and AMF inoculation in both cultivars. In cultivar SST806, significant reduction of polymeric protein was observed due to application of both AMF (6%) and a combination of AMF with drought stress (4%). Regarding the two-dimensional separation of the gluten proteins, drought had the largest effect on protein spots, with AMF causing up-regulation of only some spots. SST806 was generally more drought tolerant than PAN3497.

Compositional profiling and bioefficacy studies of pulses-supplemented isocaloric designer biscuits for recently diagnosed diabetic individuals

This study addresses global concerns about diabetes mellitus by exploring a novel approach to manage hyperglycemia through pulses-supplemented designer biscuits. Control and designer biscuits were prepared with varying proportions of wheat flour and pulses (chickpea, mungbean). The pulses-supplemented biscuits exhibited increased protein content and reduced readily available carbohydrates. Selected designer biscuits, with 12.5 % incorporation of chickpea and mungbean pulse flour, demonstrated significantly lower glycemic index (69.17 ± 5.01) and higher satiety index (122.19 ± 8.85) compared to control biscuits. These showed 13 % less glycemic index and 9 % higher satiety index as compared to control biscuits. A four-week bio-efficacy trial involving diabetic subjects consuming these biscuits as a routine snack resulted in an 11.45 % decrease in fasting blood glucose and a 19.15 % reduction in random blood glucose levels. Insulin and HDL levels also significantly improved. The study concludes that these designer biscuits possess a hypoglycemic effect, offering a potential dietary intervention for managing diabetes.

C-terminal Domain of T4 gene 32 Protein Enables Rapid Filament Reorganization and Dissociation

Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA (ssDNA) binding protein essential for DNA replication. gp32 forms stable protein filaments on ssDNA through cooperative interactions between its core and N-terminal domain. gp32′s C-terminal domain (CTD) is believed to primarily help coordinate DNA replication via direct interactions with constituents of the replisome. However, the exact mechanisms of these interactions are not known, and it is unclear how tightly-bound gp32 filaments are readily displaced from ssDNA as required for genomic processing. Here, we utilized truncated gp32 variants to demonstrate a key role of the CTD in regulating gp32 dissociation. Using optical tweezers, we probed the binding and dissociation dynamics of CTD-truncated gp32, *I, to an 8.1 knt ssDNA molecule and compared these measurements with those for full-length gp32. The *I-ssDNA helical filament becomes progressively unwound with increased protein concentration but remains significantly more stable than that of full-length, wild-type gp32. Protein oversaturation, concomitant with filament unwinding, facilitates rapid dissociation of full-length gp32 from across the entire ssDNA segment. In contrast, *I primarily unbinds slowly from only the ends of the cooperative clusters, regardless of the protein density and degree of DNA unwinding. Our results suggest that the CTD may constrain the relative twist angle of proteins within the ssDNA filament such that upon critical unwinding the cooperative interprotein interactions largely vanish, facilitating prompt removal of gp32. We propose a model of CTD-mediated gp32 displacement via internal restructuring of its filament, providing a mechanism for rapid ssDNA clearing during genomic processing.

The Growth &amp; Survival of Tilapia Fry (Oreochronis niloticus) with the Addition of Fermented Coconut Pulp to Feed

Feed is one of the components that support the aquaculture business, hence the available feed must be sufficient and meet the needs of the fish. The use of coconut pulp as a feed ingredient is limited by its low protein content and high crude fiber content, which reduces the feed's digestibility level. Fermentation is a technology used to increase protein content and reduce crude fiber content, and increase protein digestibility. A. adding fermented coconut pulp 65%, commercial feed 35%, B. adding fermented coconut pulp 45%, commercial feed 55%, C. adding fermented coconut pulp 25%, 75% commercial feed, D. Control (100% commercial feed). This study aimed to analyze the effect of adding fermented coconut pulp to the commercial feed on the growth and survival of tilapia fry (Orechromis niloticus). The research is an experimental study using a Completely Randomized Design (CRD) with 4 treatments and 3 replications for 12 experimental units. The results showed that the addition of fermented coconut pulp to tilapia feed had no significant effect on absolute weight and absolute length growth, but had a significant effect on the survival rate of tilapia fry. The best dose of adding fermented coconut pulp to the feed was found in the 25% treatment. The addition of fermented coconut pulp up to 45% did not make a significant difference with the use of commercial feed (control). This indicates the addition of fermented coconut pulp up to 45% can still be given to fish, hence it can be recommended that the addition of fermented coconut pulp to the feed should not exceed 45%.

Comparative transcriptome combined with physiological analyses revealed key genes and pathways for cadmium tolerance in wild-type and mutant microalgae Dunaliella salina

Cadmium (Cd) has become one of the major contaminants in aquatic ecosystems. The microalgae Dunaliella salina is known as a cadmium (Cd) accumulator and shows a high level of tolerance to Cd, but improved Cd tolerance and relevant mechanisms are still required for investigation. In this study, two mutant strains (M1 and M2) of D. salina with high Cd resistance were obtained by chemical mutagenesis. In the normal medium, there were no differences between the two mutant strains and the wild-type strain in biochemical and physiological indicators. However, the two mutant strains showed high Cd resistance, rapid growth rate, and good Cd accumulation capacity in the medium containing high levels of Cd compared with the wild strain, which may be involved in increases in protein content, accumulation of carotenoids and chlorophylls, and glutathione reductase (GR) activity. We identified 829, 587, and 740 differentially expressed genes (DEGs) from the Cd/Control, Cd + M1/Cd, and Cd + M2/Cd sample groups by transcriptome analysis, respectively. The main DEGs were found in the ribosome, DNA replication, photosynthesis-antenna, and photosynthesis pathways after KEGG enrichment. There were 39 DEGs involved in the photosynthesis and photosynthesis-antenna pathways, of which 28 DEGs were down-regulated by Cd in the wild-type strain but remained relatively unchanged in the mutant strains. Under Cd exposure, the mutant strains showed higher mRNA levels of most DEGs involved in the ribosome and DNA replication pathways than the wild strain. Taken together, the present study will help us understand the mechanism of Cd tolerance in microalgae, and provide candidate genes and key pathways for phytoremediation.

Exploration and Profiling of Potential Thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp. from varied Soil of Delhi region, India and their Plant Growth-Promoting Traits

Soilless cultivation has emerged as a fundamental alternative for large-scale vegetable production because it generates high-quality yields and uses resources efficiently. While plant growth-promoting bacteria (PGPB) are known to enhance growth and physiological aspects in crops grown in soil, their application in soilless cultivation has been relatively less explored. This study aimed to isolate potential PGPBs from soil samples collected from five locations in and around the Delhi-National Capital Region (NCR), India, which were further screened for significant PGPB attributes. Among these, 51 isolated were selected for assessing the impact on Oryza sativa (rice) growth and yield grown on a hydroponic set. The results indicated that isolates AFSI16 and ACSI02 significantly improved the physiological parameters of the plants. For instance, treatment with AFSI16 showed a 23.27% increase in maximum fresh shoot mass, while ACSI02 resulted in a 46.8% increase in root fresh mass. Additionally, ACSI02 exhibited the highest shoot length (34.07%), whereas AFSI16 exhibited the longest root length (46.08%) in O.sativa. Treatment with AFSI16 also led to significant increases in total protein content (4.94%) and chlorophyll content (23.44%), while ACSI02 treatment showed a 13.48% increase in maximum carotenoid content in the leaves. The potential PGPBs were identified through 16S rRNA sequencing, as the two most effective strains, AFSI16 and ACSI02, belonged to thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp., respectively. This study demonstrated the potential of these identified PGPB strains in enhancing crop performance, specifically in soilless cultivation systems.

Estimation and Association of Total Protein Concentration with Early Childhood Caries in 3-6-year-old Children: A Randomized Clinical Trial.

Caries is a common problem witnessed in children, early childhood caries (ECC) is the most predominant chronic disease which not only leads to distress and pain but also poor quality of life, thus affecting the overall well-being of children. Salivary protein plays a vital part in monitoring health status or disease. It was stated that the salivary proteins could regulate the equilibrium of oral health, preserve a stable ecosystem, and constrain the growth of cariogenic bacteria. The aim of this study is to estimate the total protein concentration in saliva and its correlation to ECC. A total of 20 patients with ECC in the age-group of 3-6 years were selected as the experimental group and 20 patients without caries for the control group. Unstimulated saliva samples were collected and subjected to spectrophotometry. The data obtained was subjected to statistical analysis. Independent student's t-test was used for the comparison of mean salivary pH between the caries group and the control group. Mann-Whitney test was used for a comparison of salivary total protein concentrations between the two groups. The mean pH of the carious group showed a statistically significant slightly lower value than that of the noncarious group. On the contrary, the mean total protein concentration of the carious group presented a statistically significant higher value than that of the noncarious group. Age-wise comparison of mean salivary proteins in the carious group and the noncarious group showed an increase in the protein concentration in the children aged 4 years or younger. Based on the results of this study, it can be concluded that there is a strong association between the total protein concentration in saliva and ECC. There exists a significant increase in the total protein concentration in children with ECC. As age increases, total protein concentration decreases with age. Total protein concentration and particular protein estimation and quantification help us in assessing the risk of caries in children at the earliest and prevention of caries through preventive measures. Estimation of total salivary protein concentration in children can be a marker for ECC in children. Thimmegowda U, Pai S, Chikkanarasaiah N, et al. Estimation and Association of Total Protein Concentration with Early Childhood Caries in 3-6-year-old Children: A Randomized Clinical Trial. Int J Clin Pediatr Dent 2024;17(1):36-40.

Chemical Characteristics of cull Ongole Beef Sausage with Substitution of Tapioca and Soy Bean Flour

The research aims to analyze the effect of adding tapioca and soybean flour (Glycine max) with different levels on the chemical characteristics of cull Ongole beef sausages. This research used the experiment method with Completely Randomized Design (CRD) method which consisted of five treatments and three replications so that there were 15 experimental units. The treatment consisted of tapioca with soybean flour formula (P0=20%:0%, P1=15%:5%, P2= 10%:10%, P3=5%:15%, P4= 0%:20%). The variables measured are water content, protein content, fat content, and cholesterol content. The results of the research showed that the substitution of soybean flour with tapioca flour had a very significant effect (P&lt;0.01) on water, protein, fat and cholesterol levels. In conclusion, soybean flour substitution had an influence on the chemical quality of beef sausages, at formula levels, soybean flour substitution resulted in a decrease in water content and fat content and an increase in protein content, as well as a decrease in cholesterol levels in line with the increase in soybean flour substitution.

Impacts of exogenous C6-HSL on the enhanced cometabolic removal of sulfadiazine by enriched nitrifying sludge

The antibiotics in the wastewater could be effectively removed by the cometabolism of nitrifying sludge mediated by ammonia monooxygenase (AMO). The effects of quorum sensing (QS) system regulated by N-acyl homoserine lactones (AHLs) on the performance and functional microorganisms in wastewater treatment have also been increasingly reported, yet few studies report the impacts of AHLs on the cometabolic removal of antibiotics by nitrifying bacteria. In this study, N-hexanoyl-L-homoserine lactone (C6-HSL) and sulfadiazine (SDZ) were selected, and a series of batch tests were implemented to study the cometabolic degradation of SDZ by enriched nitrifying sludge and the responses of the sludge under different concentrations of exogenous C6-HSL (0.1, 0.5 and 1 μM). The results indicated C6-HSL enhanced the cometabolic removal of SDZ by nitrifying sludge, with the highest removal efficiency of 84.60 % achieved with 1 μM C6-HSL. The extracellular polymeric substances (EPS) increased by 50.8 %–139.5 %，and a positive correlation between the increase of protein content in EPS and the concentration of C6-HSL was established. C6-HSL also brought an increase in cellular adenosine triphosphate content, which increased by 51.8 % with 1 μM C6-HSL. Moreover, C6-HSL up-regulated the amoA gene and enhanced AMO activity, which led to the sufficient degradation of SDZ.