Increase In Erythrocytes Research Articles

Hematological parameters of lactating cows when use the feed additives Live protein and Tanrem Sh

The purpose of the research was to study the hematological parameters of lactating cows when use the feed additives Live protein and Tanrem Sh. The scientific and economic experiment was carried out at the breeding farm of Razliv LLC in the Kurgan region on 30 matured lactating cows of Blackand- White breed. Animals were selected into groups using the analog method, taking into account age, live weight, calving date, and productivity. The experiment was carried out during 2022–2023. Analyzing changes in individual blood parameters, a tendency towards an increase in erythrocytes in the experimental groups by 14.62–14.82 % was observed, compared to the control group). At the same time,erythrocytes in animals of the experimental groups were more saturated with hemoglobin. For example, if in the control group the hemoglobin content in erythrocytes was at the level of 92.03 g/l, then in the experimental groups its quantity was 101.16–11.44 g/l or 9.92–10.22 % higher. The content of leukocytes in all groups was from 7.55 to 8.19×109/l, with the maximum permissible value of 12.0×109/l. The content of lymphocytes in animals of the experimental and control groups did not have large deviations and was from 4.58 to 5.08×109/l with the norm from 2.5 to 7.5×109/l. Eosinophils are an indicator of allergic processes. Their content was also within the permissible norms and was from 7.50 to 8.90 %. Thus, the inclusion of the additives Live protein and Tanrem Sh in the ration of lactating cows had a positive eff ect on hematological parameters. The increase in the number of erythrocytes with increased hemoglobin saturation compared to the control group indicates more intensive metabolic processes in the animals’ bodies.

Preventive and therapeutic vascular photobiomodulation decreases the inflammatory markers and enhances the muscle repair process in an animal model

Photobiomodulation therapy (PBM) has shown positive effects when applied locally to modulate the inflammatory process and facilitate muscle repair. However, the available literature on the mechanisms of action of vascular photobiomodulation (VPBM), a non-invasive method of vascular irradiation, specifically in the context of local muscle repair, is limited. Thus, this study aimed to assess the impact of vascular photobiomodulation (VPBM) using a low-level laser (LLL) on the inflammatory response and the process of skeletal muscle repair whether administered prior to or following cryoinjury-induced acute muscle damage in the tibialis anterior (TA) muscles. Wistar rats (n = 85) were organized into the following experimental groups: (1) Control (n = 5); (2) Non-Injury + VPBM (n = 20); (3) Injured (n = 20); (4) Pre-VPBM + Injury (n = 20); (5) Injury + Post-VPBM (n = 20). VPBM was administered over the vein/artery at the base of the animals' tails (wavelength: 780 nm; power: 40 mW; application area: 0.04 cm2; energy density: 80 J/cm2). Euthanasia of the animals was carried out at 1, 2, 5, and 7 days after inducing the injuries. Tibialis anterior (TA) muscles were collected for both qualitative and quantitative histological analysis using H&E staining and for assessing protein expression of TNF-α, MCP-1, IL-1β, and IL-6 via ELISA. Blood samples were collected and analyzed using an automatic hematological analyzer and a leukocyte differential counter. Data were subjected to statistical analysis (ANOVA/Tukey). The results revealed that applying VPBM prior to injury led to an increase in circulating neutrophils (granulocytes) after 1 day and a subsequent increase in monocytes after 2 and 5 days, compared to the Non-Injury + VPBM and Injured groups. Notably, an increase in erythrocytes and hemoglobin concentration was observed in the Non-Injury + VPBM group on days 1 and 2 in comparison to the Injured group. In terms of histological aspects, only the Prior VPBM + Injured group exhibited a reduction in the number of inflammatory cells after 1, 5, and 7 days, along with an increase in blood vessels at 5 days. Both the Prior VPBM + Injured and Injured + VPBM after groups displayed a decrease in myonecrosis at 1, 2, and 7 days, an increase in newly-formed and immature fibers after 5 and 7 days, and neovascularization after 1, 2, and 7 days. Regarding protein expression, there was an increase in MCP-1 after 1 and 5 days, TNF-α, IL-6, and IL-1β after 1, 2, and 5 days in the Injured + VPBM after group when compared to the other experimental groups. The Prior VPBM + Injured group exhibited increased MCP-1 production after 2 days, in comparison to the Non-Injury + VPBM and Control groups. Notably, on day 7, the Injured group continued to show elevated MCP-1 protein expression when compared to the VPBM groups. In conclusion, VPBM effectively modulated hematological parameters, circulating leukocytes, the protein expression of the chemokine MCP-1, and the proinflammatory cytokines TNF-α and IL-1β, ultimately influencing the inflammatory process. This modulation resulted in a reduction of myonecrosis, restoration of tissue architecture, increased formation of newly and immature muscle fibers, and enhanced neovascularization, with more pronounced effects when VPBM was applied prior to the muscle injury.

Comparison of the effectiveness of substitution therapy preparations in bronchopneumonia of calves

The results of research on the methods of bronchopneumonia treatment of young cattle are presented. It is noted that the disease is associated with the effect of a number of different etiological conditions (lower air temperature, air humidification, and air pollution, etc.) on the animal's body. Bronchopneumonia of calves is registered at livestock complexes in cold and wet seasons as a seasonal disease. In this regard, the development of effective treatment schemes for this pathology is an urgent task. The effectiveness of the use of substitution therapy preparations in the treatment of bronchopneumonia of calves was studied. The study was conducted at a farm in the Altai Territory in the autumn period. For realization of this experiment two equal groups (experimental and control) of 5 calves in each were formed according to the principle of analogies. The treatment regimen for both groups included Resflor and Loxic 2% preparations. Calves of the control group received VitOkey as a substitution therapy, the experimental group – Vitam. Clinical signs and blood morphologic analysis were evaluated during the experiment. Clinical examination of the calves showed the following signs: elevated body temperature, wheezing, coughing, foci of blunting in the lungs in the anterior and posterior lobes, apathy, nasal discharge, loss of appetite, erythropenia (up to (4.6 ± 0.2) × 1012/l), leukocytosis (up to (16.5 ± 0.6) × 109/l), increased ECR (up to 1.2 ± 0.1 mm/h), neutrophilic leukocytosis with a leftward shift of the nucleus, and monocytopenia. By the 5th day of treatment, the calves of the experimental group had no pulmonary rales, coughing, and the body temperature was within normal limits. On the 7th day, there was an increase in erythrocytes ((6.4 ± 0.3) × 1012/l), a decrease in leukocytes ((10.6 ± 0.3) × 109/l) and ESR (0.4 ± 0.1 mm/h). The use of vitamin-amino acid complex in complex therapy of bronchopneumonia of calves reduces the duration and severity of the course of the disease.

Brewer’s yeast as a diet supplement in carp aquaculture: impact on production coefficients and haematological and biochemical plasma parameters

While brewer’s yeast (Saccharomyces cerevisiae), a rich source of proteins and B vitamins, is commonly used as an animal feed supplement, its use in aquaculture has been limited. Here, we assess its impact on 192 individuals of common carp (Cyprinus carpio) production and haematological and biochemical parameters from blood plasma using a control group fed commercial feed with 20% soybean meal and three test groups fed commercial feed with (i) 17.50% soybean + 2.50% brewer’s yeast (group B-2.5), (ii) 15% soybean + 5% yeast (group B-5) and (iii) 10% soybean and 10% yeast (group B-10). At the end of the experiment, all fish were weighed and measured and subsequently blood samples were taken from eight fish in each tank. There was a significant increase in total length (3%) and weight (5.37%) in group B-5. While fish muscle nitrogenous matter and protein efficiency ratio increased slowly across experimental groups, Fulton’s and Clark’s condition factors changed minimally (range 2.73–2.89%). Apparent net protein utilisation increased to 17.63% in B-10, suggesting that increasing yeast levels result in improved nitrogen utilisation. All fish muscle fat levels were within optimal range (4.16–4.68%) and, while there was a significant increase in erythrocytes in B-5 (up 0.40 T.L−1), haemoglobin levels increased only slightly in B-5 and B-10 (average 4.50 g.L−1) and B-10 had the lowest number of leukocytes (6.8 G.L−1). No significant changes were recorded in blood plasma biochemical parameters. Our results suggest that a 5% brewer’s yeast feed supplement represents a useful protein source for carp aquaculture.

Features of the immune status of patients with acute coronary syndrome who underwent СOVID-19, depending on the number of cytotoxic T lymphocytes (CD8<sup>+</sup>)

The 2019 coronavirus disease (COVID-19) pandemic has had an unprecedented impact on health and economies around the world. Direct myocardial injury and cytokine storm, leading to destabilization of preexisting plaques and accelerated formation of new plaques, are two mechanisms that trigger the acute coronary syndrome in COVID-19. There is insufficient data on the immune status of patients with acute coronary syndrome who have undergone COVID-19. The aim of the study was to study T and B cell, humoral immunity depending on the number of cytotoxic T lymphocytes (CD8+) in patients with acute coronary syndrome who underwent COVID-19. Materials and methods of research: 65 men with unstable angina pectoris and acute myocardial infarction (acute coronary syndrome) from 40 to 65 years old, who had previously had COVID-19, were examined. A study of peripheral blood was carried out: complete blood count (Medonic device, Sweden), general and specific IgM, IgG, IgA, compliment fragments (Vector Best, Russia). Subpopulations of T and B lymphocytes were determined by flow cytometry. In persons with acute coronary syndrome who underwent COVID-19 with predominantly normal and elevated levels of cytotoxic T cells, a more severe course of the disease was observed: patients with acute myocardial infarction prevailed, they had longer mortality, longer treatment duration, and stent thrombosis was more common. In patients with elevated cytotoxic T cells, there was a maximum increase in erythrocytes, hemoglobin, hematocrit, lymphocytes of both the total number and subpopulations – T helpers, T-NK lymphocytes, NK lymphocytes, T lymphocytes of early and late activation, B1 and B2 lymphocytes, index of NBT-induced test. In patients with normal levels of NK cells, compared with other groups, there was an increase in spontaneous NBT activity and index, a significant decrease in C3a and C5a complement fragments. Prevalence of stent thrombosis and mortality in the group of patients with normal levels of cytotoxic T cells may indicate torpidity of the immune system in these patients with poor outcomes.

Screening of different doses of lyophilized beetroot for their hematopoietic potential.

Background: Beetroots are considered a vital source of nutrition and are usually incorporated into the daily diet to prevent different disease conditions. The therapeutic effect observed is due to the presence of active ingredients in the vegetables. The majority of our population, especially females, suffers from anemia due to malnutrition. The perseverance of this research was to explore the hematopoietic effects of lyophilized beetroot powder at different doses so they could be recommended as a nutritional supplement.
 Methodology: Albino rabbits weighing 1500-2000 gm of either gender were selected for the study and alienated into three sets. Set I was administered distilled water, whereas sets II and III were given lyophilized beetroot 500 mg/kg and 1000 mg/kg, correspondingly. For hematopoietic evaluation of blood, automated huma count plus was utilized. After adjusting according to their weights, lyophilized beetroot powder was suspended in distilled water and given at 500 mg/kg and 1000 mg/kg to rabbits. They were dosed once daily for two months.
 Results: The effects were verified before the dosing was scheduled and on the 30th day and 60th day of dosing. The outcomes presented a highly noteworthy increase (p<0.0001) in hemoglobin, erythrocyte count, hematocrit, leukocyte count, and thrombocytes by both doses. 500 mg/kg dose showed a more significant increase in leukocytes and thrombocyte count compared to 1000 mg/kg, whereas 1000 mg/kg showed a more substantial increase in erythrocytes and Hb as compared to 500 mg/kg at the conclusion of the study period.
 Conclusion: From the above, it can be concluded that beetroot possesses pronounced hematopoietic effects and might be beneficial as a non-pharmacological treatment for anemia and thrombocytopenia.

Influence of the phytosorption complex on the milk productivity of the zaanen goats

It is most rational to use complex preparations, thereby solving several problems at once. Firstly, there is an antimicrobial effect on the microflora, and secondly, sorption and evacuation of myctoxins and other toxigenic substances are carried out.The purpose of the experiment was to study the effect of a new phytosorption complex on the milk production of goats. The studies were carried out on goats of the Saanen breed, in one of the farms of the Leningrad region. Two groups of animals were created with 10 animals each. Previously, in order to create equivalent ones, the grading of animals was carried out according to their productivity.Goats of the experimental group, in addition to the basic diet, were added a phytosorption complex at a dose of 4% of the daily intake of feed. The experiment time was 30 days. The effects of the phytosorption complex on milk production indicators were taken into account. The biochemical properties of milk were determined according to GOST 32949-2014. The study of blood was carried out according to standard methods, using hardware technologies. The obtained data were processed using the STATISTICA 6.0 program, the differences were considered statistically significant at p<0.05.The experiment showed that the addition of the phytosorption complex to the diet had a positive effect on the body of experimental animals, which affected milk productivity, the increase in the experimental group was 25%. The positive effect of the phytosorption complex is also evidenced by blood indicators, where an increase in erythrocytes by 9.8% and hemoglobin by 13.28% can be noted, which may indicate an increase in the respiratory function of the blood. An increase in immunoglobulins indicates an increase in the immune status of experimental animals. An increase in IgA by 8.9% indicates an increase in the protective functions of the mucous membranes, an increase in the possibility of sorption of microorganisms.Thus, the introduction of phytosorption complexes into the diet of goats is an expedient and definitely promising direction in the prevention of both infectious diseases and toxicosis.

Hematological Profile and Spleen Histology Improvement in Diabetic Rats Treated with PLGA Nanoparticles-Ethanol Extract of Jengkol (Archidendron pauciflorum) Fruit Peel

Jengkol (Archidendron pauciflorum) fruit peel-ethanol extract (JFP) contains several chemical compounds that act as antioxidant agents and are considered to have the potential to treat chronic disorders such as diabetes mellitus. Plant extract is generally applied in polylactic-co-glycolic acid (PLGA) nanoparticles due to its efficiency in entering the target organs. This study aims to investigate the effect of PLGA nanoparticles-ethanol extract of jengkol fruit peel (PLGA nanoparticle-JFP) on improving the hematological profile and the spleen histology of streptozotocin-induced diabetic rats. The treatment group was divided into six groups: (1) control group, (2) streptozotocin control (STZ); (3) 10 mg/kg BW of glibenclamide (GLB); (4) 770 mg/kg BW of jengkol fruit peel-ethanol extract (JFP1); (5) 110 mg/kg BW of JFP (JFP2); and (6) 770 mg/kg BW PLGA nanoparticle-JFP (PLGA-JFP). PLGA-JFP group showed a significant increase in erythrocytes and leukocytes counts (7.73x106 ± 0.02 cell/mm3 and 9.68x103 ± 3.0 cell/mm3 respectively), a decrease in lymphocytes and neutrophils percentage (66.5%±0.5% and 28±1.4% respectively) compared to the STZ group, and no significant difference in monocyte, eosinophils, and basophils percentage within groups. On the spleen histology, the white pulp diameter and the red pulp area showed significantly smaller (168.31±10.69μm and 8,01.28± 480.33 μm2 respectively) compared to the STZ group. Almost all parameters showed no significant difference compared to the GLB group but are significantly different from the control group. The administration of PLGA nanoparticle-JFP was proven to reverse hematological parameters and improve the spleen histology but has not yet reversed the diabetic rats’ condition back to normal.

Productivity and hematological parameters of blood of young pigs at fatten-ing for feeding protein vitamin mineral supplement

The article presents data on the productivity and hematological parameters of the blood of young pigs for fattening for the inclusion in their diet of a new protein vitamin-mineral supplement with essential oils, Efiprot. Plant essential oils are natural phytobiotics containing fat-soluble vitamins, polyunsaturated fatty acids, volatile acids, aldehydes, and other biologically active substances with bactericidal and antiseptic properties, increased appetite, and secretion of digestive juices. Thus, they provide better feed conversion and increased productivity. Protein vitamin-mineral supplement Efiprot is for a diet consisting of barley grains (50 %) and wheat grains (35 %). The experiment was carried out on two groups (12 heads in a group) of young pigs, a cross of Large White and Landrace. At the beginning of the experiment live weight of the animals was 35 kg; at the end, it was 110 kg. Control group animals were fed a supplement without essential oils; experimental group animals were additionally fed a dose of essential oils (200 g per 1 ton of supplement). Feeding supplements with essential oils positively affected pig productivity and feed growth. The absolute increase in live animals of the experimental group averaged 68.6 kg, average daily was 762 g; the corresponding values of the control group were 63.8 kg and 709 g for 90 days. Feeding protein vitamin-mineral supplement (PVMS) increased average daily gain by 53 g or 7.48 %, while feed consumption per 1 kg of live weight gain was lower by 0.3 energy feed units or 6.69 % than the control one. Hematological parameters of pig blood fed by PVMS Efiprot corresponded to the limits of the physiological norm. At the end of the experiment, an increase in erythrocytes, total protein, and hemoglobin was observed in the blood of the experimental animals, which is consistent with an increase in meat productivity. A slight increase in the mass of basophils, eosinophils, and leukocytes is adaptive and incredible.

Faktor-Faktor yang Mempengaruhi Kepatuhan Ibu Hamil dalam Mengkonsumsi Tablet Fe: Literature Review

Anemia in pregnant women is a condition where there is a lack of red blood cells or hemoglobin. A condition where in the first and third trimesters there is an increase in plasma volume in a greater proportion than the increase in erythrocytes, resulting in a decrease in hemoglobin concentration (Hb) which causes hemoglobin levels to fall below 11.0 g/dl or in the second trimester < 10.5 g/dl. dl due to hemodilution. The purpose of this literature review study was to determine the factors that influence the compliance of pregnant women in consuming Fe tablets. This research method used a literature review method and uses databases such as Pubmed, Google Scholar and Health Profiles with a vulnerable publication period of the last 10 (ten) years. The results from several journals show that there were several factors that influence the compliance of pregnant women in consuming Fe tablets, namely knowledge, attitudes, family support, support from health workers, frequency of ANC visits, and age. Some of these factors show a significant relationship to the effect of pregnant women's compliance in consuming Fe tablets. Conclusion: the factors that influence the compliance of pregnant women in consuming Fe tablets are knowledge, attitudes, family support, support from health workers, frequency of ANC visits, and age.

Исследование влияния «Промевита», витаминно-минеральной добавки, на производство молока и репродуктивные показатели молочных коров

At the present time vitamin-mineral and energy cows' feed additives made from natural components gain more and more popularity. The work considers the influence of the complex feeding additive "Promevit" on the indices of raw milk quality of cows and also milk productivity and indices of reproduction of animals. At adding the feed additive to the basic diet an increase in haemoglobin level of experimental cows by 21.4%, an increase in erythrocytes by 14.5%, a moderate increase in white blood cells, a slight increase (by 5.2%) in protein content in the blood of experimental cows and an increase by 6, 6% of albumin level, relatively initial indicators, increase of urea content by 23,1% within reference values, decrease of aspartataminotransferase, as well as glutamate dehydrogenase, that shows restoration of hepatobiliary system work. The milk yield of the experimental group cows was 285 kg higher than that of the control group and the percentage of milk fat was 1.3 times higher. On average the mass fraction of fat in milk of the cows from the experimental group was 3.32%, which was 4.2% higher than in the control group. There was a 10.9% increase in the amount of milk protein in raw milk compared to the initial data. The milk fat yield increased by 19.5 kg relative to the control group as well as the protein yield increased by 13.9 kg. With the use of the dietary supplement no complicated forms of hyperkeratosis were recorded in the experimental group cows, while in the control group cows' number increased by 20.7%. The number of teats with uncomplicated forms of hyperkeratosis also increased by 20% relative to baseline data. In the experimental group cows had a reduction of udder quarters with signs of clinical mastitis, the level of latent mastitis remained practically unchanged. A positive effect of the feed additive on the course of the parturition and postpartum period in cows was also noted.

Systemic Effects of Photobiomodulation on Blood Components in the Treatment of Community-Acquired Pneumonia.

Background: The analysis of the complete blood count (CBC) of patients with community-acquired pneumonia (CAP) is an essential practice both for diagnosing the disease and for evaluating the patient's clinical evolution. It is proposed in the present study to analyze the hematological alterations resulting from photobiostimulation using near-infrared light-emitting diodes (LEDs) in patients with CAP. Methods: This was a clinical, prospective, blinded, and descriptive longitudinal study that involved 21 patients undergoing CAP treatment who were divided into two groups: LED, 11 patients who were treated with infrared LED and conventional treatment; and CON (control), 10 patients who received only conventional treatment (antibiotic therapy and physiotherapy). Physiotherapy was applied before LED irradiation in the LED group. The patients' CBCs were obtained before and after treatment, and erythrocyte counts, hemoglobin and hematocrit concentrations, and leukocyte and platelet counts were assessed. The phototherapy was performed with a vest with an array of 300 LEDs (940 nm) mounted on an area of 36 × 58 cm and positioned in the patient's anterior thoracic and abdominal regions. The total power was 6 W, with 15 min of irradiation time. The patients were treated daily for seven consecutive days. Statistical analyses of the intra- and intergroups of CBC data were done using Student's t-test and one-way ANOVA (analysis of variance), respectively, both at the significance level of α = 0.05. Results: There was a statistically significant recovery difference after treatment in the LED group compared with the CON group for erythrocytes, hemoglobin, leukocytes, segmented and band neutrophils, lymphocytes, and monocytes (p < 0.05). The greatest differences between the LED and CON groups were lymphocyte count reduction (60% vs. 16%), erythrocyte increase (86% vs. 35%), and leukocyte reduction (28% vs. 15%). Conclusions: The hematologic components of CAP patients recovered their normal values faster with conventional treatment associated with infrared LED therapy, thus indicating greater treatment efficiency when compared with the conventional therapy. This study was registered with the Brazilian Registry of Clinical Trials (ReBeC) under Universal Trial Number (UTN) U1111-1229-1296 (2019/06/05).

NEW VITAMIN-MINERAL PREPARATION FOR PREVENTION OF OBSTETRIC PATHOLOGY OF ANIMALS «BIOTAN 3Z»

The article presents the vitamin and mineral preparation «Biotan 3Z" which includes micro- and macroelements in the form of water-soluble salts, namely Calcium, Phosphorus, Magnesium, Iron, Copper Manganese, Copper, Cobalt and Manganese. It also contains many vitamins (aspartic acid, glutamic acid, vitamin A, E, B1, PP) and essential amino acids - rich in methionine, lysine, arginine, histidine, tyrosine, phenylalanine, isoleucine, leucine, valine, cystine, tryptophan, glycine, proline, threonine and serine, which are necessary for the normal functioning of the body. Due to this veterinary drug the level of diseases of domestic animals decreases, their productive qualities increase. This drug accelerates the growth of animals. Vitamin and mineral preparation «Biotan 3Z" reduces the incidence and mortality of animals. Prevents diseases that originate from changes in housing conditions or occur against the background of metabolism or lack of vitamins and minerals.&#x0D; When using this drug in bitches, the biochemical and morphological parameters of the blood of these animals were analyzed. Significant changes in blood parameters such as erythrocytes, hemoglobin, hematocrit, total protein, before and after administration of the drug in various forms of manifestation have not been established. There was only a slight increase in erythrocytes from 6,6±0,2 thousand / ml to 7,8±0,4 thousand/ml, as well as a slight increase in hemoglobin from 120±3,8 g/l to 146±6,8 g/l. Vitamins and minerals contained in the fed drug had a positive effect on metabolism in the mother and fetus, as evidenced by changes in blood creatinine, which at 14 weeks before delivery was 127,6 μmol/l and on the first day of delivery in the experimental group increased to 135.8 μmol/l.&#x0D; The addition of a vitamin-mineral preparation to the main diet in some way affected the metabolism and biochemical composition of the blood of the bitches of the experimental group. It was expressed by an increase in the physiological limits of glucose, total protein, albumin, creatinine, cholesterol, total calcium, inorganic phosphorus and a decrease in globulins, bilirubin and AST. The concentration of urea, triglycerides and LF activity almost did not change.&#x0D; Feeding of vitamin-mineral preparation «Biotan 3Z» to bitches before childbirth affected the content in the blood of experimental animals of total calcium and inorganic phosphorus, the concentration of which increased by 0,2 mmol / l compared to baseline, and 0,2 and 0,3 mmol/l – with the control group after the experiment. Vitamin and mineral preparation «Biotan 3Z» is of interest for veterinary medicine and therefore it is advisable to continue its further clinical study.

Vitamin D supplementation on anemia markers and the impact on PTH in patients with chronic kidney disease

Chronic Kidney Disease (CKD) is an injury that causes progressive impairment of exocrine and endocrine renal functions. A very common complication is anemia, caused by reduced erythropoietin production, iron deficiency and inflammation. Evidence demonstrates that vitamin D has effects on anemia of inflammation, through the increase in erythrocytes and decrease in pro-inflammatory cytokines. This study aims to review the effects of vitamin D supplementation on 25(OH)D2 concentrations, on anemia markers and on PTH levels. This is an integrative review carried out through the search and selection of original publications, in english and portuguese, indexed in PubMed, Web of Science and Science Direct databases belonging to the 2010-2020 range. The results pointed to 25(OH)D2 concentrations compatible with normality after vitamin D supplementation. In five studies, there was no change in hemoglobin and PTH levels, and in four studies there was a reduction in the dose of EPO or erythroid stimulating agent, attributing such effect on the role of calcitriol as a substrate for bone marrow erythropoietic cells. The study concluded that vitamin D supplementation had beneficial effects for correction of 25(OH)D2 deficiency, however, it reinforces the controversy about the behavior of vitamin D on the improvement of anemia markers and PTH levels in patients with DRC. Therefore, it is suggested that the beneficial effect of vitamin D on anemia in renal patients may be independent of PTH suppression.

Use Effect of Unconventional Feed and Mineral Additives on Animal and Poultry Productivity

The possibilities of increasing productivity of animals and poultry by using an additive containing selenium, rapeseed cake and complex mineral additive have been studied. An addition of the preparation “Kartsesel” in mixed fodder promoted the increase in digestibility of nutrients, the increase in erythrocytes, hemoglobin in blood, output of conditioned youngsters, profitability of production. Conditioned young growth was obtained more by 19-22 %. Inclusion in mixed fodder at cultivation of goose rapeseed presscake of Siberian selection positively affected the increase of live weight, yield of gutted carcass. Goose of the first experimental group surpassed the control group by live weight by 3.4%, and the second experimental group by 6.1%. Output of gutted carcass of goose receiving rapeseed meal and rapeseed meal in combination with rapeseed oil is 286.13 g and 388.48 g more. Complex mineral additive in the diet of bulls contributed to the increase of live weight of bulls in 15 months of age, slaughter output, profitability of production and improvement of meat quality. The live weight of the control group of bulls not receiving the feed additive made 459.50 kg, and the experimental group made 11.00 kg more. At the same time the level of profitability of production on steers of the experimental group receiving a mineral additive amounted to 13.77% instead of 10.24% in the control group.

Dietary supplementation with curcumin-loaded nanocapsules in lambs: Nanotechnology as a new tool for nutrition.

Curcumin-containing nanocapsule powder formulations have not been used in ruminant feed to date, despite the fact that curcumin is known to be a functional food additive. The objective of this study was to determine whether ethyl polymethacrylate (Eudragit L-100) nanocapsules loaded with curcumin (N-CU) would improve health and growth of lambs. Thirty-two male Lacaune lambs (body weight [BW] = 16 ± 0.99 kg; 45 d of age) were randomly assigned to 1 of 4 treatments: T0, T1, T2 and T4, representing supplementation of curcumin at 0, 1, 2, and 4 mg/kg concentrate, respectively. The animals in each treatment were allocated in 4 pens of 2 lambs each (8 lambs per treatment). The experiment lasted 17 d, with samples and measurements collected on d 0, 7, 12, and 17. The T2 lambs had greater average daily gain than T0 lambs. Regression analysis showed that the ideal dose of N-CU to enhance weight gain was 1.89 mg/kg concentrate. There were significant interactions (P < 0.05) between treatments × time for hematological variables, particularly for increases in erythrocytes (T2) and reductions in counts of leukocytes, neutrophils, and lymphocytes in T1 and T2. There were significant interactions between treatment × time for total protein, globulin, urea, and triglyceride levels. Stimulation of the antioxidant system was also observed. There were increased levels of non-protein thiols (NPSH), as well as increased activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) in the supplemented animals. Levels of reactive oxygen species (ROS) were lower in the serum of supplemented lambs. In general, the 4 mg/kg dose had no positive effects on growth or health. This was an unexpected result, given the known properties of curcumin. Taken together, these findings suggest that addition of low concentrations of nanoencapsulated curcumin (T1 and T2) in lamb feed improves health, minimizing oxidative stress and generates anti-inflammatory effects that may have contributed indirectly to greater weight gain. Nanocapsules potentiate the effects of curcumin and may emerge as a new tool in animal nutrition.

Influence of the prebiotic feed additive “Vetokislinka” the microflora of the feces and hematological parameters of calves of milk period

The results of studying the effect of the prebiotic feed additive “Vetokislinka” on the microflora of feces, hematological parameters and the growth rate of calves of the milk period are presented. Calves of the control group received the basic diet without the inclusion of the studied prebiotic. The calves of the experimental groups were fed with the prebiotic Vetokislinka in a dose of 0.8 in addition to the basic diet; 1.0 and 1.2 ml per 1 liter of drinking water from 6-10 to 90 days of age, daily frequency, 2 times a day (50% of the daily value). According to the research results, it was found that an effective dose of the prebiotic feed additive “Vetokislinka” was a dose of 1 ml per 1 liter of drinking dose, which had a positive effect on the microbial landscape of feces in calves, in which there was a significant increase in the number of normal flora: lactobacilli and bifidobacteria, as well as a decrease in Escherichia. Within the physiological norm, the use of the prebiotic “Vetokislinka” promoted an increase in erythrocytes by 9.1%, hemoglobin by 11.3%, platelets by 15.2% compared to the first control group (p < 0.05). The average daily gain of calves was significantly higher by 9.8%, while reducing feed costs per 1 kg of live weight by 8.8%. The economic efficiency from the introduction of the prebiotic supplement “Vetokislinka” into the diet of calves, at a dose of 1 ml / head per 1 liter of drinking water, per calf was 561.6 rubles.

Increase the productivity of animals and poultry when use non-traditional feed and mineral additives

The possibilities of increasing the productivity of animals and poultry when using an additive containing selenium, rapeseed cake and a complex mineral additive have been studied. Adding to the feed of selenium-containing drug “Carasel” enhanced the digestibility of nutrients, increase of erythrocytes, hemoglobin in the blood, yield of certifi ed goslings, and profi tability. The digestibility of dry and organic matter has increased by 0,46–1,14 %, protein – up to 5,15 %. Depending on the dose of the additive the digestibility of raw fi ber, raw fat, and nitrogen-free extractive substances were higher. Certifi ed goslings have been received 19–22 % more. The inclusion of rapeseed cake of Siberian selection in compound feed when rearing goslings had a positive eff ect on the growth of live weight, the output of the gutted carcass. Goslings of the 1st experimental group exceeded the live weight of goslings of the control group by 3,4 %, and the 2nd experimental group by 6,1 %. The weight of the semi-gutted carcass was higher in the experimental groups receiving rapeseed presscake and rapeseed presscake in combination with rapeseed oil, and exceeded the weight of carcasses of the control group by 298,22 and 388,48 g (P ≤ 0,001), respectively. The weight of gutted carcasses was also significantly higher in the experimental groups. A complex mineral additive in the ration of steers of Hereford breed contributed to the increase in their live weight at the age of 15 months, slaughter yield, profitability of production and improvement of beef quality. The pre-slaughter weight of steers in the experimental group was 465,67 kg and was by 19,0 kg or 4,25 % more than that of steers in the control group (P ≤ 0,001). Studies have shown that the carcass weight was higher in the experimental group than in the control group by 12,09 kg or 4,39 % (P ≤ 0,001). The slaughter yield was 1,08 % higher. Economic evaluation of the use of feed additive in the experiment in the steers of Hereford breed has shown that the use of the developed mineral feed additives contributed to the increase in live weight of steers and the profi tability of production. The level of profitability in the group of steers experimental group receiving comprehensive mineral additive amounted to 13,80 % in comparison with the control group where it was equal to 9,40 %.

Inhibition of TGF\u03b2 improves hematopoietic stem cell niche and ameliorates cancer-related anemia

BackgroundCancer cachexia is a wasting syndrome that is quite common in terminal-stage cancer patients. Cancer-related anemia is one of the main features of cancer cachexia and mostly results in a poor prognosis. The disadvantages of the current therapies are obvious, but few new treatments have been developed because the pathological mechanism remains unclear.MethodsC57BL/6 mice were subcutaneously injected with Lewis lung carcinoma cells to generate a cancer-related anemia model. The treated group received daily intraperitoneal injections of SB505124. Blood parameters were determined with a routine blood counting analyzer. Erythroid cells and hematopoietic stem/progenitor cells were analyzed by flow cytometry. The microarchitecture changes of the femurs were determined by micro-computed tomography scans. Smad2/3 phosphorylation was analyzed by immunofluorescence and Western blotting. The changes in the hematopoietic stem cell niche were revealed by qPCR analysis of both fibrosis-related genes and hematopoietic genes, fibroblastic colony-forming unit assays, and lineage differentiation of mesenchymal stromal cells.ResultsThe mouse model exhibited hematopoietic suppression, marked by a decrease of erythrocytes in the peripheral blood, as well as an increase of immature erythroblasts and reduced differentiation of multipotent progenitors in the bone marrow. The ratio of bone volume/total volume, trabecular number, and cortical wall thickness all appeared to decrease, and the increased osteoclast number has led to the release of latent TGFβ and TGFβ signaling over-activation. Excessive TGFβ deteriorated the hematopoietic stem cell niche, inducing fibrosis of the bone marrow as well as the transition of mesenchymal stromal cells. Treatment with SB505124, a small-molecule inhibitor of TGFβ signaling, significantly attenuated the symptoms of cancer-related anemia in this model, as evidenced by the increase of erythrocytes in the peripheral blood and the normalized proportion of erythroblast cell clusters. Meanwhile, hindered hematopoiesis and deteriorated hematopoietic stem cell niche were also shown to be restored with SB505124 treatment.ConclusionThis study investigated the role of TGFβ released by bone remodeling in the progression of cancer-related anemia and revealed a potential therapeutic approach for relieving defects in hematopoiesis.

High Adenosine Deaminase Level Among Healthy Probands of Diamond Blackfan Anemia (DBA) Cosegregates With the DBA Gene Region on Chromosome 19q13

AbstractPhenotypic characterization of Diamond Blackfan Anemia (DBA) patients and their relatives was performed in 54 families. Complete blood count, fetal hemoglobin level, erythrocyte i antigen expression, and erythrocyte adenosine deaminase (eADA) activities were quantitated in patients and relatives. eADA was elevated in 28 of 34 transfusion-independent DBA patients, whereas persistence of erythrocyte i antigen was noticed in only 10 of 20 DBA patients. High eADA activities were also found in 14 of 149 healthy family members, allowing us to identify an isolated high eADA phenotype in these families. In contrast, increase in erythrocyte i antigen expression, elevated fetal hemoglobin levels, and macrocytosis were much less frequently noted in nonaffected members of the DBA families studied. Importantly, isolated high eADA phenotype was found to be significantly associated with genetic markers on chromosome 19 that segregate with the DBA phenotype. Isolated high eADA phenotype thus seems to reflect a silent phenotype of DBA in affected families. These findings suggest that elevated eADA activity in unaffected individuals needs to be taken into account during genetic assessment of DBA families and could be used for accurate assessment of mode of inheritance.