Inconsistent Changes Research Articles

Brain-derived neurotrophic factor (BDNF) changes in rodent models of schizophrenia induced by ketamine: a systematic review.

Schizophrenia is a severe neuropsychiatric disorder ranking among the top ten global disability causes. In rodents, sub-chronic or chronic ketamine treatment is used for the induction of schizophrenia. Ketamine affects the function of brain-derived neurotrophic factor (BDNF), the most important neurotrophin involved in the pathophysiology of different neuropsychiatric disorders. The present systematic review aimed to investigate BDNF changes in rodent studies used ketamine-induced schizophrenia. PubMed electronic database was searched and 44 articles were found. After removal of unrelated articles, 17 articles were selected. The results showed a wide range of inconsistent changes in BDNF levels. We found that sub-chronic and chronic ketamine treatment in rats decreased BDNF or had no effect. Sub-chronic and chronic ketamine treatment in mice only decreased BDNF. However, increased BDNF was commonly observed following acute ketamine treatment. These results showed the possible role of species and the duration of treatment. Also, sex can also be involved in BDNF changes because one study showed inconsistent BDNF changes in male and female rats. In conclusion, we found that ketamine's effects may depend on factors such as duration of administration, sex, and species. Therefore, the wide range of BDNF changes may be related to high variability in methods. Because of this variability, there is currently no standardized method for using ketamine as a rodent model for schizophrenia. Further research is needed to establish a standardized pharmacological model of schizophrenia using ketamine treatment.

Biomechanical optimization of RGB LED systems: Temperature prediction model and optical compensation algorithm based on built-in constant current source chip

In the realm of modern applications, especially those related to human - involved scenarios such as vehicles and medical or wearable devices, the performance of RGB LED lights is of great significance. Beyond the traditional concerns of electronic - optical properties, the integration of biomechanics - related factors can bring new perspectives and optimizations. In vehicles, during prolonged illumination, the color shifts of various LED lights not only affect driving comfort but also have potential implications for driving safety from a biomechanical perspective. The human body’s visual and psychological responses to these color and luminance changes are complex. For example, sudden or inconsistent color changes can cause visual fatigue and distraction, which may impact a driver’s reaction time and decision - making ability, all of which are related to biomechanical aspects of human - machine interaction. Each LED shows deviations in wavelength and luminous intensity, and the light decay of RGB chips is inconsistent. To address these issues and ensure consistent color and luminance of all RGB ambient lights during use, this paper proposes a temperature prediction model (PT - model) based on constant current source output PWM. This model takes into account the impact of temperature changes on the color coordinates and luminous flux of LED lights. Moreover, considering the potential applications in medical and wearable devices, the model’s performance becomes even more crucial. In medical light - based treatment devices, the precise control of RGB LED temperature and light output is essential for ensuring the effectiveness of treatment while minimizing potential harm to human tissues. Biomechanics research can provide insights into how different tissues respond to light - induced heat and mechanical stress. Similarly, in wearable devices for health monitoring, the stability of RGB LED performance is related to the comfort and accuracy of the device’s operation on the human body. Experimental results show that compared to traditional models, the prediction accuracy of this model is significantly improved, with errors reduced to within 4.3%. The model’s effectiveness is verified under different ambient temperatures ranging from −40 ℃ to 120 ℃, which is crucial for ensuring its reliability in various real - world applications, especially those related to human - centered scenarios influenced by biomechanical factors.

In-Depth Proteomic Analysis of Tissue Interstitial Fluid Reveals Biomarker Candidates Related to Varying Differentiation Statuses in Gastric Adenocarcinoma.

The proteomic heterogeneity of gastric adenocarcinoma (GC) has been extensively investigated at the bulk tissue level, which can only provide an average molecular state. In this study, we collected an in-depth quantitative proteomic dataset of tissues and interstitial fluids (ISFs) from both poorly and non-poorly differentiated GC and presented a comprehensive analysis from several perspectives. Comparison of proteomes between ISFs and tissues revealed that ISF exhibited higher abundances of proteins associated with blood microparticles, protein-lipid complexes, immunoglobulin complexes, and high-density lipoprotein particles. Also, consistent and inconsistent protein abundance changes between them were revealed by a correlation analysis. Interestingly, a more pronounced difference between tumors and normal adjacent tissues was found at the ISF level, which accurately reflected tissue properties compared to those of bulk tissue. Two ISF-derived biomarker candidates, calsyntenin-1 (CLSTN1) and prosaposin (PSAP), were identified by distinguishing patients with different differentiation statuses and were further validated in serum samples. Additionally, the silencing of CLSTN1 and PSAP was demonstrated to suppress cell proliferation, migration, and invasion in poorly differentiated gastric cancer cell lines. In summary, the ISF proteome offers a new perspective on tumor biology. This study provides a valuable resource that significantly enhances the understanding of GC and may ultimately benefit clinical practice.

Non-Negligible Factors Influence Tree-Ring-Based Temperature Reconstruction and Comparison over Mid-Latitude China

Warm-season mean maximum temperature changes over mid-latitude regions have been attracting increasing attention amid the background of global warming. In this study, we present three tree-ring width chronologies: Tongbai Mountain (TBM; 1916–2014), Shimen Mountain (SMM; 1663–2014), and Xinlong (XL; 1541–2014), derived respectively from the eastern Qinling Mountains, north–central China, and the eastern Tibetan Plateau. Therein, TBM and SMM are newly developed, while XL is a reanalysis. Correlation analysis with climatic factors reveals that these three chronologies exhibit the highest correlation with the May–July mean maximum temperature. Based on these chronologies, we conducted reconstructions of the May–July mean maximum temperature. Spatial correlation analysis of each reconstruction with concurrent observed data, as well as comparisons with nearby temperature reconstructions, indicates their large-scale representativeness. However, during the common period of 1916–2014, the three chronologies show weak correlations with each other at the interannual timescale. Furthermore, the 11-year running correlation coefficients among the three reconstructions fluctuated during this common period. Additionally, fluctuations were observed between the reconstructions from SMM and XL during the overlapping period of 1668–2009, suggesting that tree-ring-based temperature reconstructions may be inconsistent when compared over mid-latitude China. These inconsistent changes can be attributed to the regional differences in the May–July mean maximum temperature change, the influence of different precipitation signals on the maximum temperature, and the El Niño–Southern Oscillations.

Feeding nursery pigs diets containing peroxidized soybean oil has minimal effects on oxidative status but dramatically reduces serum vitamin E concentrations.

A subgroup of pigs from two experiments (EXP) were selected to evaluate the impact of pigs fed diets containing peroxidized soybean oil (SO) on plasma-based measures of oxidative stress and vitamin E. Pigs were fed diets containing SO that was either unprocessed (23 °C; peroxide value of 3 meq/kg and an anisidine value of 4) or thermally processed at 135 °C for 42h (peroxide value of 30 meq/kg and an anisidine value of 501). The corn-soybean meal-based diets contained either 10% SO (EXP 1) or 8% SO (EXP 2). Pigs were fed the experimental diets for 22 d (EXP 1, 13.5 to 24.0kg, 2 pigs/pen) or 27 d (EXP 2, 21.3 to 37.5kg, 1 pig/pen), each with 10 replications per dietary treatment. Pigs fed diets containing the peroxidized SO had reduced ADG, ADFI, and GF compared to pigs fed diets containing the unheated SO (P ≤ 0.01). Pigs fed diets containing peroxidized SO had increased plasma concentrations of F2-isoprostanes and reactive oxygen metabolites compared to pigs fed diets containing unheated SO (P ≤ 0.01). In contrast, plasma thiobarbituric acid reactive substances concentrations tended to decrease in pigs fed diets containing peroxidized SO compared to pigs fed diets containing unheated SO (P = 0.10). There was no apparent effect of pigs consuming diets containing peroxidized SO on plasma antioxidant adsorbent capacity or an oxidative stress index (P ≥ 0.19). Pigs fed diets containing peroxidized SO resulted in a reduction in plasma vitamin E compared to pigs fed diets containing unheated SO (P ≤ 0.01). Results indicate that adding SO that has been thermally processed thereby containing high concentrations of aldehydes resulted in inconsistent changes of markers of oxidative stress, but dramatically reduced plasma vitamin E concentrations.

Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities.

Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities.

PERANCANGAN EVALUASI PRODUKTIVITAS KERJA KARYAWAN DENGAN METODE SISTEM PENILAIAN KINERJA TERINTEGRASI (IPMS) DAN ANALYTICAL HIERARCHY PROCESS (AHP) DALAM PENGAMBILAN KEPUTUSAN DI UD. BIMASAKTI TIMUR RAYA SURABAYA

UD. Bimasakti Timur Raya Surabaya, there is an issue of delays in starting work caused by employees not beginning their tasks on time and believing that these delays will not affect their salary or other benefits. The Integrated Performance Measurement System (IPMS) of UD. Bima Sakti Timur Raya focuses solely on financial and sales KPIs, without involving other elements such as internal business processes and development aspects. The percentage of achievement and realization of employee potential improvements in profit enhancement shows a high variation and meets the established target of 15.20%. UD. Bima Sakti Timur Raya demonstrates performance changes by using the Integrated Performance Measurement System (IPMS) and has so far succeeded in meeting the predetermined target of 15.20%. UD. Bima Sakti Timur Raya has successfully achieved its goals, but this effort has still not been able to meet the set target for reducing customer complaints. UD. Bima Sakti Timur Raya experiences inconsistent changes every year, and the company still has not been able to achieve the set targets. UD. Bima Sakti Timur Raya shows growth each year, but the company has yet to meet the established goals.

Postcholecystectomy Gut Microbiome Changes and the Clinical Impact: A Systematic Review With Narrative Synthesis.

Cholecystectomy (CCE) can affect the enterohepatic circulation of bile acids and result in gut microbiome changes. This systematic review aimed to clarify the effect of CCE on gut microbiome composition and its clinical impact. A systematic search was conducted in PubMed, Web of Science, and Scopus, combining keywords such as "cholecystectomy" or "post-cholecystectomy" with "gut microbiome," "stool microbiome," or "gut dysbiosis." Data were extracted and synthesized using narrative review. Study quality was assessed using the Newcastle-Ottawa scale. A total of 1373 articles were screened and 14 studies were selected. Significant but inconsistent microbiome changes were reported. Changes were observed in alpha and beta diversity. At phylum level, an increase in Bacteroides and Ascomycota, decrease in Firmicutes, Actinomycetes, and Basidiomycota, and both increase and decrease in Fusobacteria were reported. At genus level, an increase in Prevotella and a decrease in Faecalibacterium were reported. In post-CCE diarrhea, decreased beta diversity, a decreased F/B ratio, an increase in Prevotella, an increase in Phocaeicola vulgatus, and a decrease in Prevotella copri were noted. For post-CCE syndrome, a higher abundance of Proteobacteria and decreased Firmicutes/Bacteroides (F/B)ratio were reported. A decreased relative abundance of Bifidobacterium longum subsp. longum from controls to CCE without colonic neoplasia to CCE with colonic neoplasia, and an increased abundance of Candida glabrata from controls, to CCE without colonic neoplasia and CCE with colonic neoplasia, were reported. Patients who underwent CCE had significant gut dysbiosis. However, current studies could not clarify the detailed gut microbial structural and functional changes associated with CCE.

Perkembangan Sistem Pendidikan di Inggris

The education system in the United Kingdom has undergone significant development over time, encompassing primary, secondary, and higher education. Primary and secondary education is structured within a framework that ensures universal access and high standards, starting from mandatory education for children aged 5 to 16 years. Higher education in the UK is renowned for its quality, with prestigious universities focusing on research and professional development. Public policies, such as the Education Act of 1944 and the national curriculum introduced in 1988, have played a vital role in shaping and updating the education system. However, modern challenges such as social and economic disparities, inconsistent policy changes, and academic pressure and mental health issues among students remain ongoing concerns. With continued efforts to improve the education system, the UK continues to adapt to changes in time and global challenges.

Identifikasi Faktor Penghambat dalam Pelaksaan Standar Operasional Prosedur Kepegawaian pada Dinas Pendidikan dan Kebudayaan Kabupaten Biak Numfor

This study aims to identify Inhibiting Factors in the Implementation of SOP for Personnel at the Education and Culture Office of Biak Numfor Regency. The research method used is descriptive research using a qualitative approach. Based on the results of the study, it is known that the inhibiting factors for the Standard Operating Procedure (SOP) for Personnel Administration Services at the Education and Culture Office of Biak Numfor Regency are the availability of adequate human, financial, and technological resources that can support the smooth implementation of SOPs are inadequate, Commitment to the monitoring and evaluation system has not been effective in identifying problems and making necessary improvements. Performance Measurement, is significantly less than optimal because frequent or inconsistent policy changes can cause confusion and difficulty in following the established SOPs, limited human resources, facilities and infrastructure can hinder the implementation of SOPs properly, lack of adequate training or understanding of SOPs can hinder staff's ability to follow established procedures.

Cortical structural network characteristics in non-cognitive impairment end-stage renal disease.

Explore alterations in topological features of gray matter volume (GMV) and structural networks in non-cognitive impairment end-stage renal disease (Non-CI ESRD). Utilizing graph theory, we collected structural magnetic resonance imaging (sMRI) data from 38 Non-CI ESRD patients and 50 normal controls (NC). We compared, and extracted the GMV across subject groups, constructed corresponding structural covariance networks (SCNs), and investigated the alterations in SCNs feature parameters between groups. In Non-CI ESRD patients, The GMV were reduced in several brain regions, predominantly on the left side (p < 0.05, FWE correction). The small-world network characteristics of the patient group's brain networks showed a tendency toward regular. In a few densities, global network parameters, transitivity, (p < 0.05) was significantly increased in the ESRD group. Regional network measurements revealed inconsistent changes in regional efficiency across different brain areas. In the analysis of network hubs, the right temporal pole is likely a compensatory hub for Non-CI ESRD patients. The SCNs in Non-CI ESRD patients demonstrated reduced topological stability against targeted attacks. This study reveals that patients with renal failure exhibited subtle changes in brain network characteristics even before a decline in cognitive scores. These changes involve compensatory activation in certain brain regions, which enhances network transitivity to maintain the efficiency of whole-brain network information integration without significant loss. Additionally, the SCNs characteristics can serve as a neuroanatomical marker for brain alterations in Non-CI ESRD patients, offering new insights into the mechanisms of early brain injury in ESRD patients.

Cardiac morphology and function in patients with primary biliary cholangitis (PBC) without cirrhosis

Abstract Background Primary biliary cholangitis (PBC) is a chronic, autoimmune liver disease causing destruction of the small intralobular bile ducts in the liver. PBC patients can also develop extrahepatic manifestations. Though patients with PBC have often elevated levels of serum lipids, data concerning cardiac involvement is inconsistent and cardiovascular changes may be underdiagnosed. Purpose The purpose of the study was to assess cardiac morphology and function in patients with PBC without cirrhosis and comparison with control subjects. Methods We studied 52 females with PBC, and included in the analysis 22 females without cirrhosis, who were compared to 44 healthy female controls matched for age (53.4 ± 9.2 and 51 ± 8.3 yrs., respectively, p =0.312). Transthoracic echocardiography (TTE) was performed with the analysis of the left ventricular (LV) global longitudinal strain (GLS) and myocardial work. Additionally, impedance cardiography was conducted, with the analysis of the autonomic function using BRS (baroreceptor sensitivity), HRV (heart rate variability) and BPV (blood pressure variability). Results Systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR) differed between PBC and control group (128 ± 17 mmHg vs. 116 ± 11 mmHg, p=0.007 and 78± 12 mmHg vs. 75 ± 8 mmHg, p=0.024, and 73.5 ± 12 bpm vs. 71 ± 8 bpm , p=0.024, respectively). PBC group had larger diameter of the cardiac chambers when adjusted for body surface area (BSA), including left atrium (LA/BSA) and right ventricle (RV/BSA), as well as left ventricular mass (LWMI) and relative wall thickness (RWT), with no difference in the left ventricular end diastolic diameter (LVEDD/BSA). LV ejection fraction (EF) measured by bi-plane Simpson method was higher in PBC group. LV diastolic function parameters (E/A, E/e’) differed in patients with PBC when compared with controls, however, without signs of diastolic dysfunction. LV GLS indices (GLSAVG, GLSLAX, GLS4C, GLS2C) were significantly higher in PBC group, as well as global myocardial work indexes: global myocardial work (GWI) and global constructive work (GCW). Non-invasive autonomic assessment showed lower BPV in patients with PBC without cirrhosis, but no differences in baroreceptor sensitivity and HRV. Impedance cardiography did not show differences between both groups. Conclusion Our study shows that PBC patients without cirrhosis are characterized by remodeling and functional changes of the heart, when compared to age-matched controls. These changes could imply hyperkinetic circulation even before the development of cirrhosis in PBC patients.

Kepastian Hukum Syarat Mantan Narapidana Calon Anggota DPD Pasca Putusan Mahkamah Konstitusi Nomor 12/PUU-XXI/2023

General Election Contestation (Election) is one indicator of the implementation of the ideals of a democratic country. A democratic state certainly cannot be separated from the ideal of the rule of law, because in fact the state of law and democracy go together. This is because a state administration must be based on applicable law, and the law is implemented as much as possible for the community. In order to support the implementation of the rule of law in accordance with the wishes of the people, the concept of democracy is a concept that is no less important. In the context of statehood, it is appropriate for the voice of the people to be represented by representatives of the people in parliament, both central and regional. The people's representatives are elected through elections by stating the conditions that must be met. However, the conditions listed seem inconsistent and there are even aggrieved parties so that they do not show legal certainty. The purpose of this study is to see aspects of legal certainty regarding the requirements for ex-prisoner who want to run in election contestations, especially the selection of individual candidates for DPD members. The method used in this research is juridical-normative legal research or commonly called doctrinal legal research with statute approach and conceptual approach. The results showed that the requirements for ex-prisoner who wanted to run in the election contestation had changed several times as a result of material tests in the Constitutional Court. The change in requirements is the result of an inconsistent change in attitude by the Constitutional Court towards the existence of ex-prisoner requirements when they want to run in election contestations, including the selection of individual candidates for DPD members.

Unveiling the dynamics of intraoperative contamination in total hip arthroplasty: the discrepancy between particulate and microbial contamination in surgical site infection risk

BackgroundSurgical site infection (SSI) is a major problem following total hip arthroplasty (THA). This study investigated the impact of a standard intraoperative routine where the surgical team wears full-body exhaust suits (space suits) within a laminar airflow (LAF)-ventilated operating room (OR) on environmental contamination. Our primary objective was to identify potential modifiable intraoperative factors that could be better controlled to minimize SSI risk.MethodsWe implemented an approach involving simultaneous and continuous air sampling throughout actual primary cementless THA procedures. This method concurrently monitored both airborne particle and microbial contamination levels from the time the patient entered the OR for surgery until extubation.ResultsAirborne particulate and microbial contamination significantly increased during the first and second patient repositionings (postural changes) when the surgical team was not wearing space suits. However, their concentration exhibited inconsistent changes during the core surgical procedures, between incision and suturing, when the surgeons wore space suits. The microbial biosensor detected zero median microbes from draping to suturing. In contrast, the particle counter indicated a significant level of airborne particles during head resection and cup press-fitting, suggesting these procedures might generate more non-viable particles.ConclusionsThis study identified a significant portion of airborne particles during the core surgical procedures as non-viable, suggesting that monitoring solely for particle counts might not suffice to estimate SSI risk. Our findings strongly support the use of space suits for surgeons to minimize intraoperative microbial contamination within LAF-ventilated ORs. Therefore, minimizing unnecessary traffic and movement of unsterile personnel is crucial. Additionally, since our data suggest increased contamination during patient repositioning, effectively controlling contamination during the first postural change plays a key role in maintaining low microbial contamination levels throughout the surgery. The use of sterile gowns during this initial maneuver might further reduce SSIs. Further research is warranted to investigate the impact of sterile attire on SSIs.

Differential effects of haloperidol on neural oscillations during wakefulness and sleep

Differential effects of haloperidol on neural oscillations during wakefulness and sleep

A TDOA sequence estimation method of underwater sound source based on hidden Markov model

A TDOA sequence estimation method of underwater sound source based on hidden Markov model

Body mass changes of dabbling and diving ducks wintering in California

AbstractBird body mass is often used as an index of body condition and fluctuates throughout the year in response to environmental conditions and avian life‐history events. We examined the body mass of 59,572 ducks representing 13 species (7 dabbling duck species and 6 diving duck species) harvested within the 3 regions of the Central Valley in California, USA (Sacramento Valley, Suisun Marsh, San Joaquin Valley). Data collection occurred in winter during 5 hunting seasons (2014–2015, 2015–2016, 2017–2018, 2018–2019, 2019–2020). For all species, age, and sex classes, the body mass of dabbling ducks varied temporally and was lowest at the end of the hunting season in late January, declining from the beginning of the hunting season in mid‐October (northern pintail [Anas acuta]: −11.4% to −20.4%; northern shoveler [Spatula clypeata]: −3.5% to −17.3%; cinnamon teal [Anas cyanoptera]: −5.0% to −17.1%; American wigeon [Mareca americana]: −6.9% to −12.2%; American green‐winged teal [Anas carolinensis]: −5.9% to −11.9%; mallard [Anas platyrhynchos]: −1.8% to −9.6%; gadwall [Mareca strepera]: −2.2% to −8.4%). As expected, adults (after hatch‐year) were heavier than immature (hatch‐year) birds (within males: 0.6–8.6%; within females: 0.1–6.6%) and males were heavier than females (within adults: 3.1–38.6%; within immatures: 5.4–33.5%) at the end of the hunting season in all species. Within dabbling duck species, body masses did not differ among regions at the beginning of the hunting season but were heavier in the Sacramento Valley (0.5–14.4%) than other areas by the end of the hunting season. In contrast, body masses of diving ducks did not vary substantially during the hunting season or among regions. Diving ducks demonstrated inconsistent changes in mass from the beginning to the end of the season (lesser scaup [Aythya affinis]: 3.4% to 12.3%; canvasback [Aythya valisineria]: 5.1% to 6.1%; bufflehead [Bucephala albeola]: −3.2% to 1.6%; ring‐necked duck [Aythya collaris]: −4.9% to 2.6%; common goldeneye [Bucephala clangula]: −3.2% to −2.4%; ruddy duck [Oxyura jamaicensis]: −16.5% to 5.5%). The substantial temporal and spatial differences in dabbling duck body masses suggest that habitat quality (as measured by caloric value of the available food) or quantity may decline during winter and varies regionally within California's Central Valley.

Associations of ACE I/D and AGTR1 rs5182 polymorphisms with diabetes and their effects on lipids in an elderly Chinese population

BackgroundDiabetes mellitus is generally accompanied by dyslipidaemia, but inconsistent relationships between lipid profiles and diabetes are noted. Moreover, genetic variations in insertion/deletion (I/D) polymorphisms at angiotensin-converting enzyme gene (ACE) and T/C polymorphisms in the angiotensin type 1 receptor gene (AGTR1) are related to diabetes and lipid levels, but the associations are controversial. Thus, the current research aimed to explore the effects of ACE I/D, AGTR1 rs5182 and diabetes mellitus on serum lipid profiles in 385 Chinese participants with an average age of 75.01 years.MethodsThe ACE I/D variant was identified using the polymerase chain reaction (PCR) method, whereas the AGTR1 rs5182 polymorphism was identified using the PCR-based restriction fragment length polymorphism (PCR-RFLP) method and verified with DNA sequencing. Total cholesterol (TC), triglyceride (TG), apolipoprotein A (ApoA), apolipoprotein B (ApoB), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels were measured using routine methods, and the lipid ratios were calculated.ResultsACE I/D, but not AGTR1 rs5182, was a predictor of TG/HDL-C for the whole study population. Both ACE I/D and AGTR1 rs5182 were predictors of HDL-C and LDL-C levels in females but not in males. Moreover, in females, diabetes mellitus and ACE I/D were identified as predictors of TG and TG/HDL-C, whereas AGTR1 rs5182 and diabetes mellitus were predictors of TG/HDL-C. Moreover, diabetes mellitus and the combination of ACE I/D and AGTR1 rs5182 variations were predictors of TG and TG/HDL-C exclusively in females.ConclusionsThe results demonstrated the potential for gender-dependent interactions of ACE I/D, AGTR1 rs5182, and diabetes on lipid profiles. These findings may serve as an additional explanation for the inconsistent changes of blood lipids in individuals with diabetes mellitus, thereby offering a novel perspective for the clinical management of blood lipid levels in diabetic patients.

Induced pluripotent stem cells derived renal tubular cells from a patient with pseudohypoparathyroidism and its response to parathyroid hormone stimulation.

Creating induced pluripotent stem cells (iPSCs) from somatic cells of patients with genetic diseases offers a pathway to generate disease-specific iPSCs carrying genetic markers. Differentiating these iPSCs into renal tubular cells can aid in understanding the pathophysiology of rare inherited renal tubular diseases through cellular experiments. Two Japanese patients with Pseudohypoparathyroidism (PHP), a 49-year-old woman and a 71-year-old man, were studied. iPSC-derived tubular cells were established from their peripheral blood mononuclear cells (PBMCs). We examined changes in intracellular and extracellular cyclic adenosine monophosphate (cAMP) levels in these cells in response to parathyroid hormone (PTH) stimulation. Renal tubular cells, differentiated from iPSCs of a healthy control (648A1), showed a PTH-dependent increase in both intracellular and extracellular cAMP levels. However, the renal tubular cells derived from the PHP patients' iPSCs showed inconsistent changes in cAMP levels upon PTH exposure. We successfully created disease-specific iPSCs from PHP patients' PBMCs, differentiated them into tubular cells, and replicated the distinctive response of the disease to PTH in vitro. This approach could enhance our understanding of the pathophysiology of inherited renal tubular diseases and contribute to developing effective treatments.

Transient characterization of the mode switching process in the reversible solid oxide cell stack

Transient characterization of the mode switching process in the reversible solid oxide cell stack